LOCUS       DAJSBM010000001       145295 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJSBM010000001 DAJSBM010000000
VERSION     DAJSBM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 145295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 145295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..145295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.001"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(12..1535)
                     /locus_tag="ONT67_000001"
     CDS             complement(12..1535)
                     /locus_tag="ONT67_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2353773.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="ONT67_000002"
     CDS             1787..2968
                     /locus_tag="ONT67_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2353774.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT67_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="ONT67_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR2353775.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="ONT67_000004"
     CDS             complement(5053..7764)
                     /locus_tag="ONT67_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353776.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="ONT67_000005"
     CDS             complement(7774..8475)
                     /locus_tag="ONT67_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353777.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="ONT67_000006"
     CDS             8661..9260
                     /locus_tag="ONT67_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2353778.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="ONT67_000007"
     CDS             9341..10354
                     /locus_tag="ONT67_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2353779.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT67_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="ONT67_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR2353780.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT67_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="ONT67_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR2353781.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="ONT67_000010"
     CDS             11802..13175
                     /locus_tag="ONT67_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2353782.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="ONT67_000011"
     CDS             13532..15676
                     /locus_tag="ONT67_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2353783.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="ONT67_000012"
     CDS             complement(15728..16768)
                     /locus_tag="ONT67_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2353784.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="ONT67_000013"
     CDS             complement(17045..18847)
                     /locus_tag="ONT67_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353785.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="ONT67_000014"
     CDS             complement(18861..19613)
                     /locus_tag="ONT67_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353786.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="ONT67_000015"
     CDS             complement(19622..19984)
                     /locus_tag="ONT67_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2353787.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="ONT67_000016"
     CDS             complement(20160..21032)
                     /locus_tag="ONT67_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2353788.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="ONT67_000017"
     CDS             complement(21058..21570)
                     /locus_tag="ONT67_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCR2353789.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="ONT67_000018"
     CDS             complement(21708..22499)
                     /locus_tag="ONT67_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCR2353790.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="ONT67_000019"
     CDS             complement(22554..23162)
                     /locus_tag="ONT67_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCR2353791.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="ONT67_000020"
     CDS             complement(23155..23472)
                     /locus_tag="ONT67_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2353792.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="ONT67_000021"
     CDS             complement(23628..24356)
                     /locus_tag="ONT67_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR2353793.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="ONT67_000022"
     CDS             24515..25396
                     /locus_tag="ONT67_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2353794.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="ONT67_000023"
     CDS             complement(25633..26676)
                     /locus_tag="ONT67_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2353795.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLYFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="ONT67_000024"
     CDS             complement(26679..27347)
                     /locus_tag="ONT67_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2353796.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="ONT67_000025"
     CDS             27489..29825
                     /locus_tag="ONT67_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2353797.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="ONT67_000026"
     CDS             complement(29940..30251)
                     /locus_tag="ONT67_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353798.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="ONT67_000027"
     CDS             30432..31907
                     /locus_tag="ONT67_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCR2353799.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="ONT67_000028"
     CDS             31954..32538
                     /locus_tag="ONT67_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR2353800.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="ONT67_000029"
     CDS             32540..32959
                     /locus_tag="ONT67_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCR2353801.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="ONT67_000030"
     CDS             complement(33240..33524)
                     /locus_tag="ONT67_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCR2353802.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="ONT67_000031"
     CDS             complement(33876..34586)
                     /locus_tag="ONT67_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCR2353803.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="ONT67_000032"
     CDS             complement(34583..36055)
                     /locus_tag="ONT67_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2353804.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="ONT67_000033"
     CDS             36193..36666
                     /locus_tag="ONT67_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2353805.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="ONT67_000034"
     CDS             complement(36819..37370)
                     /locus_tag="ONT67_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2353806.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="ONT67_000035"
     CDS             37475..38005
                     /locus_tag="ONT67_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCR2353807.1"
                     /translation="MKEFKELEDFIGTRFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="ONT67_000036"
     CDS             complement(38488..38931)
                     /locus_tag="ONT67_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353808.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="ONT67_000037"
     CDS             complement(38947..40107)
                     /locus_tag="ONT67_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2353809.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="ONT67_000038"
     CDS             40614..42314
                     /locus_tag="ONT67_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353810.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPSFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="ONT67_000039"
     CDS             complement(42416..43249)
                     /locus_tag="ONT67_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR2353811.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="ONT67_000040"
     CDS             complement(43553..45718)
                     /locus_tag="ONT67_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2353812.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="ONT67_000041"
     CDS             45978..48272
                     /locus_tag="ONT67_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2353813.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT67_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="ONT67_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2353814.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="ONT67_000043"
     CDS             48923..49930
                     /locus_tag="ONT67_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2353815.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="ONT67_000044"
     CDS             49952..50971
                     /locus_tag="ONT67_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2353816.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT67_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="ONT67_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2353817.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="ONT67_000046"
     CDS             complement(52729..54150)
                     /locus_tag="ONT67_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353818.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT67_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="ONT67_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2353819.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="ONT67_000048"
     CDS             complement(56561..57817)
                     /locus_tag="ONT67_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR2353820.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="ONT67_000049"
     CDS             58397..59698
                     /locus_tag="ONT67_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2353821.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="ONT67_000050"
     CDS             complement(59767..60366)
                     /locus_tag="ONT67_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2353822.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="ONT67_000051"
     CDS             60620..61501
                     /locus_tag="ONT67_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353823.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="ONT67_000052"
     CDS             61514..63124
                     /locus_tag="ONT67_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCR2353824.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="ONT67_000053"
     CDS             complement(63201..63860)
                     /locus_tag="ONT67_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2353825.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="ONT67_000054"
     CDS             complement(64143..64808)
                     /locus_tag="ONT67_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCR2353826.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT67_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="ONT67_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCR2353827.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT67_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="ONT67_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR2353828.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT67_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="ONT67_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCR2353829.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="ONT67_000058"
     CDS             67122..67442
                     /locus_tag="ONT67_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR2353830.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="ONT67_000059"
     CDS             complement(67554..68423)
                     /locus_tag="ONT67_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2353831.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="ONT67_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="ONT67_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="ONT67_000061"
     CDS             complement(69819..70328)
                     /locus_tag="ONT67_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353832.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="ONT67_000062"
     CDS             complement(70503..72638)
                     /locus_tag="ONT67_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353833.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="ONT67_000063"
     CDS             complement(72721..74376)
                     /locus_tag="ONT67_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2353834.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="ONT67_000064"
     CDS             complement(74378..74692)
                     /locus_tag="ONT67_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2353835.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="ONT67_000065"
     CDS             74862..75122
                     /locus_tag="ONT67_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCR2353836.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="ONT67_000066"
     CDS             75136..75450
                     /locus_tag="ONT67_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCR2353837.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="ONT67_000067"
     CDS             complement(76180..76734)
                     /locus_tag="ONT67_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR2353838.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIRLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="ONT67_000068"
     CDS             complement(76860..78788)
                     /locus_tag="ONT67_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2353839.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="ONT67_000069"
     CDS             complement(79094..79810)
                     /locus_tag="ONT67_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2353840.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT67_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="ONT67_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCR2353841.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT67_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="ONT67_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR2353842.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="ONT67_000072"
     CDS             complement(83280..84740)
                     /locus_tag="ONT67_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353843.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="ONT67_000073"
     CDS             complement(84730..86025)
                     /locus_tag="ONT67_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2353844.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="ONT67_000074"
     CDS             complement(86357..87034)
                     /locus_tag="ONT67_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCR2353845.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="ONT67_000075"
     CDS             complement(87064..88155)
                     /locus_tag="ONT67_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCR2353846.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="ONT67_000076"
     CDS             complement(88256..88690)
                     /locus_tag="ONT67_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2353847.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT67_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="ONT67_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2353848.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="ONT67_000078"
     CDS             complement(90728..92623)
                     /locus_tag="ONT67_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353849.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="ONT67_000079"
     CDS             complement(92681..94456)
                     /locus_tag="ONT67_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2353850.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="ONT67_000080"
     CDS             complement(94506..96002)
                     /locus_tag="ONT67_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2353851.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="ONT67_000081"
     CDS             complement(96196..97818)
                     /locus_tag="ONT67_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2353852.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="ONT67_000082"
     CDS             complement(98267..99580)
                     /locus_tag="ONT67_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2353853.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="ONT67_000083"
     CDS             complement(99825..100049)
                     /locus_tag="ONT67_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353854.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="ONT67_000084"
     CDS             complement(100069..100491)
                     /locus_tag="ONT67_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCR2353855.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="ONT67_000085"
     CDS             complement(100635..101345)
                     /locus_tag="ONT67_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353856.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="ONT67_000086"
     CDS             complement(101363..101860)
                     /locus_tag="ONT67_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353857.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="ONT67_000087"
     CDS             complement(101897..102349)
                     /locus_tag="ONT67_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2353858.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="ONT67_000088"
     CDS             complement(102484..103089)
                     /locus_tag="ONT67_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2353859.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="ONT67_000089"
     CDS             complement(103539..104159)
                     /locus_tag="ONT67_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2353860.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="ONT67_000090"
     CDS             complement(104161..105030)
                     /locus_tag="ONT67_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCR2353861.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="ONT67_000091"
     CDS             complement(105053..105460)
                     /locus_tag="ONT67_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2353862.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="ONT67_000092"
     CDS             complement(105479..106768)
                     /locus_tag="ONT67_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2353863.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="ONT67_000093"
     CDS             complement(106795..107097)
                     /locus_tag="ONT67_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2353864.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="ONT67_000094"
     CDS             complement(107122..109215)
                     /locus_tag="ONT67_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2353865.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="ONT67_000095"
     CDS             complement(109704..110468)
                     /locus_tag="ONT67_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HCR2353866.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT67_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="ONT67_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HCR2353867.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="ONT67_000097"
     CDS             complement(111473..112549)
                     /locus_tag="ONT67_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR2353868.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="ONT67_000098"
     CDS             complement(112573..113166)
                     /locus_tag="ONT67_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCR2353869.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT67_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="ONT67_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCR2353870.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="ONT67_000100"
     CDS             complement(114836..115135)
                     /locus_tag="ONT67_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCR2353871.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT67_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="ONT67_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCR2353872.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="ONT67_000102"
     CDS             complement(116890..117801)
                     /locus_tag="ONT67_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2353873.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="ONT67_000103"
     CDS             117921..118778
                     /locus_tag="ONT67_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2353874.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="ONT67_000104"
     CDS             complement(118965..119327)
                     /locus_tag="ONT67_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR2353875.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="ONT67_000105"
     CDS             119673..121112
                     /locus_tag="ONT67_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353876.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="ONT67_000106"
     CDS             complement(121119..123671)
                     /locus_tag="ONT67_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HCR2353877.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="ONT67_000107"
     CDS             complement(123785..125116)
                     /locus_tag="ONT67_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353878.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="ONT67_000108"
     CDS             complement(125082..126560)
                     /locus_tag="ONT67_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353879.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT67_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="ONT67_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCR2353880.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="ONT67_000110"
     CDS             129170..130597
                     /locus_tag="ONT67_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353881.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="ONT67_000111"
     CDS             complement(130689..131054)
                     /locus_tag="ONT67_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2353882.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="ONT67_000112"
     CDS             complement(131051..131416)
                     /locus_tag="ONT67_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HCR2353883.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="ONT67_000113"
     CDS             complement(131608..131856)
                     /locus_tag="ONT67_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353884.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="ONT67_000114"
     CDS             131855..131983
                     /locus_tag="ONT67_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HCR2353885.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="ONT67_000115"
     CDS             132040..133734
                     /locus_tag="ONT67_000115"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCR2353886.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="ONT67_000116"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="ONT67_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            133834..134007
                     /locus_tag="ONT67_000117"
     CDS             133834..134007
                     /locus_tag="ONT67_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353887.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            134385..135518
                     /locus_tag="ONT67_000118"
     CDS             134385..135518
                     /locus_tag="ONT67_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR2353888.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="ONT67_000119"
     CDS             135549..135869
                     /locus_tag="ONT67_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HCR2353889.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="ONT67_000120"
     CDS             complement(135898..136362)
                     /locus_tag="ONT67_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353890.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="ONT67_000121"
     CDS             complement(136494..137747)
                     /locus_tag="ONT67_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2353891.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="ONT67_000122"
     CDS             complement(137776..138093)
                     /locus_tag="ONT67_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2353892.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="ONT67_000123"
     CDS             complement(138119..138439)
                     /locus_tag="ONT67_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2353893.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="ONT67_000124"
     CDS             complement(138903..140318)
                     /locus_tag="ONT67_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2353894.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="ONT67_000125"
     CDS             complement(140335..141651)
                     /locus_tag="ONT67_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2353895.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="ONT67_000126"
     CDS             complement(141801..142499)
                     /locus_tag="ONT67_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR2353896.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="ONT67_000127"
     CDS             complement(142788..143744)
                     /locus_tag="ONT67_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2353897.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT67_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="ONT67_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2353898.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..>145295)
                     /locus_tag="ONT67_000129"
     CDS             complement(144865..>145295)
                     /locus_tag="ONT67_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353899.1"
                     /translation="VVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQ
                     WVNLPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISG
                     VKGVADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT        42832 a        30252 c        25310 g        46901 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaataca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcgcttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatctttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatc tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaa
//
LOCUS       DAJSBM010000002       113045 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJSBM010000002 DAJSBM010000000
VERSION     DAJSBM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 113045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.002"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1458
                     /locus_tag="ONT67_000130"
     CDS             <1..1458
                     /locus_tag="ONT67_000130"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2353900.1"
                     /translation="VLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGY
                     AIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQ
                     QRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMM
                     AQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMN
                     LILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNR
                     YGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYA
                     VIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILE
                     TYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQ
                     QVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1448..2665
                     /locus_tag="ONT67_000131"
     CDS             1448..2665
                     /locus_tag="ONT67_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2353901.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2678..5827
                     /locus_tag="ONT67_000132"
     CDS             2678..5827
                     /locus_tag="ONT67_000132"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HCR2353902.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7762..8427)
                     /locus_tag="ONT67_000133"
     CDS             complement(7762..8427)
                     /locus_tag="ONT67_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353903.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8607..10589
                     /locus_tag="ONT67_000134"
     CDS             8607..10589
                     /locus_tag="ONT67_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCR2353904.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10593..11231
                     /locus_tag="ONT67_000135"
     CDS             10593..11231
                     /locus_tag="ONT67_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353905.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11259..11687
                     /locus_tag="ONT67_000136"
     CDS             11259..11687
                     /locus_tag="ONT67_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353906.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12144..13055
                     /locus_tag="ONT67_000137"
     CDS             12144..13055
                     /locus_tag="ONT67_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2353907.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            13272..13709
                     /locus_tag="ONT67_000138"
     CDS             13272..13709
                     /locus_tag="ONT67_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353908.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            13804..14688
                     /locus_tag="ONT67_000139"
     CDS             13804..14688
                     /locus_tag="ONT67_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2353909.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            14912..18079
                     /locus_tag="ONT67_000140"
     CDS             14912..18079
                     /locus_tag="ONT67_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353910.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            18099..18644
                     /locus_tag="ONT67_000141"
     CDS             18099..18644
                     /locus_tag="ONT67_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HCR2353911.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            18672..19127
                     /locus_tag="ONT67_000142"
     CDS             18672..19127
                     /locus_tag="ONT67_000142"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HCR2353912.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            19124..19882
                     /locus_tag="ONT67_000143"
     CDS             19124..19882
                     /locus_tag="ONT67_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2353913.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(19968..20708)
                     /locus_tag="ONT67_000144"
     CDS             complement(19968..20708)
                     /locus_tag="ONT67_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2353914.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            20769..22022
                     /locus_tag="ONT67_000145"
     CDS             20769..22022
                     /locus_tag="ONT67_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2353915.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            22344..22571
                     /locus_tag="ONT67_000146"
     CDS             22344..22571
                     /locus_tag="ONT67_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2353916.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            22694..23020
                     /locus_tag="ONT67_000147"
     CDS             22694..23020
                     /locus_tag="ONT67_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2353917.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            23088..23801
                     /locus_tag="ONT67_000148"
     CDS             23088..23801
                     /locus_tag="ONT67_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2353918.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            24111..24962
                     /locus_tag="ONT67_000149"
     CDS             24111..24962
                     /locus_tag="ONT67_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2353919.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            25083..26948
                     /locus_tag="ONT67_000150"
     CDS             25083..26948
                     /locus_tag="ONT67_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353920.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            26996..28405
                     /locus_tag="ONT67_000151"
     CDS             26996..28405
                     /locus_tag="ONT67_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR2353921.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            28513..29214
                     /locus_tag="ONT67_000152"
     CDS             28513..29214
                     /locus_tag="ONT67_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2353922.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            29373..29852
                     /locus_tag="ONT67_000153"
                     /pseudo
     CDS             29373..29852
                     /locus_tag="ONT67_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            30700..31275
                     /locus_tag="ONT67_000154"
     CDS             30700..31275
                     /locus_tag="ONT67_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCR2353923.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            31275..31715
                     /locus_tag="ONT67_000155"
     CDS             31275..31715
                     /locus_tag="ONT67_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2353924.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            31731..32432
                     /locus_tag="ONT67_000156"
     CDS             31731..32432
                     /locus_tag="ONT67_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2353925.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(32920..33345)
                     /locus_tag="ONT67_000157"
     CDS             complement(32920..33345)
                     /locus_tag="ONT67_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353926.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            33719..34690
                     /locus_tag="ONT67_000158"
     CDS             33719..34690
                     /locus_tag="ONT67_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2353927.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            34722..36119
                     /locus_tag="ONT67_000159"
     CDS             34722..36119
                     /locus_tag="ONT67_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2353928.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            36278..36901
                     /locus_tag="ONT67_000160"
     CDS             36278..36901
                     /locus_tag="ONT67_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353929.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            37650..39743
                     /locus_tag="ONT67_000161"
     CDS             37650..39743
                     /locus_tag="ONT67_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2353930.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            39903..40769
                     /locus_tag="ONT67_000162"
     CDS             39903..40769
                     /locus_tag="ONT67_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2353931.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40791..41228)
                     /gene="ndk"
                     /locus_tag="ONT67_000163"
     CDS             complement(40791..41228)
                     /gene="ndk"
                     /locus_tag="ONT67_000163"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR2353932.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            41887..44565
                     /gene="mgtA"
                     /locus_tag="ONT67_000164"
     CDS             41887..44565
                     /gene="mgtA"
                     /locus_tag="ONT67_000164"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR2353933.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            44652..45323
                     /locus_tag="ONT67_000165"
     CDS             44652..45323
                     /locus_tag="ONT67_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2353934.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     misc_binding    45400..45633
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    45676..45914
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            45970..47043
                     /locus_tag="ONT67_000166"
     CDS             45970..47043
                     /locus_tag="ONT67_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2353935.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            47045..47725
                     /locus_tag="ONT67_000167"
     CDS             47045..47725
                     /locus_tag="ONT67_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353936.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            47742..48581
                     /locus_tag="ONT67_000168"
     CDS             47742..48581
                     /locus_tag="ONT67_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2353937.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            48612..49835
                     /locus_tag="ONT67_000169"
     CDS             48612..49835
                     /locus_tag="ONT67_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCR2353938.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            50238..51656
                     /gene="nhaC"
                     /locus_tag="ONT67_000170"
     CDS             50238..51656
                     /gene="nhaC"
                     /locus_tag="ONT67_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCR2353939.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(52160..52603)
                     /locus_tag="ONT67_000171"
     CDS             complement(52160..52603)
                     /locus_tag="ONT67_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCR2353940.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(52821..54161)
                     /locus_tag="ONT67_000172"
     CDS             complement(52821..54161)
                     /locus_tag="ONT67_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353941.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            54430..55326
                     /locus_tag="ONT67_000173"
     CDS             54430..55326
                     /locus_tag="ONT67_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCR2353942.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(55476..56417)
                     /locus_tag="ONT67_000174"
     CDS             complement(55476..56417)
                     /locus_tag="ONT67_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2353943.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            56543..57664
                     /locus_tag="ONT67_000175"
     CDS             56543..57664
                     /locus_tag="ONT67_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2353944.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            57922..59424
                     /gene="eat(A)"
                     /locus_tag="ONT67_000176"
     CDS             57922..59424
                     /gene="eat(A)"
                     /locus_tag="ONT67_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2353945.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            59452..60018
                     /locus_tag="ONT67_000177"
     CDS             59452..60018
                     /locus_tag="ONT67_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2353946.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            60229..61290
                     /locus_tag="ONT67_000178"
     CDS             60229..61290
                     /locus_tag="ONT67_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2353947.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            61293..62189
                     /gene="murQ"
                     /locus_tag="ONT67_000179"
     CDS             61293..62189
                     /gene="murQ"
                     /locus_tag="ONT67_000179"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2353948.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            62203..64185
                     /locus_tag="ONT67_000180"
     CDS             62203..64185
                     /locus_tag="ONT67_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2353949.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(64250..64696)
                     /gene="nrdI"
                     /locus_tag="ONT67_000181"
     CDS             complement(64250..64696)
                     /gene="nrdI"
                     /locus_tag="ONT67_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR2353950.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            64907..66331
                     /locus_tag="ONT67_000182"
     CDS             64907..66331
                     /locus_tag="ONT67_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2353951.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(66512..67285)
                     /locus_tag="ONT67_000183"
     CDS             complement(66512..67285)
                     /locus_tag="ONT67_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2353952.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(67282..68187)
                     /locus_tag="ONT67_000184"
     CDS             complement(67282..68187)
                     /locus_tag="ONT67_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2353953.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(68341..68661)
                     /locus_tag="ONT67_000185"
     CDS             complement(68341..68661)
                     /locus_tag="ONT67_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCR2353954.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            69135..69821
                     /locus_tag="ONT67_000186"
     CDS             69135..69821
                     /locus_tag="ONT67_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCR2353955.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGIASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            69823..70767
                     /locus_tag="ONT67_000187"
     CDS             69823..70767
                     /locus_tag="ONT67_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCR2353956.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            70966..71610
                     /gene="pcp"
                     /locus_tag="ONT67_000188"
     CDS             70966..71610
                     /gene="pcp"
                     /locus_tag="ONT67_000188"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCR2353957.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            71689..72966
                     /locus_tag="ONT67_000189"
     CDS             71689..72966
                     /locus_tag="ONT67_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353958.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(73150..73899)
                     /locus_tag="ONT67_000190"
     CDS             complement(73150..73899)
                     /locus_tag="ONT67_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCR2353959.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            74229..74609
                     /locus_tag="ONT67_000191"
     CDS             74229..74609
                     /locus_tag="ONT67_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2353960.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            74705..75214
                     /locus_tag="ONT67_000192"
     CDS             74705..75214
                     /locus_tag="ONT67_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353961.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            75291..75470
                     /locus_tag="ONT67_000193"
     CDS             75291..75470
                     /locus_tag="ONT67_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353962.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            75495..76088
                     /locus_tag="ONT67_000194"
     CDS             75495..76088
                     /locus_tag="ONT67_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353963.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(76135..76650)
                     /locus_tag="ONT67_000195"
     CDS             complement(76135..76650)
                     /locus_tag="ONT67_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353964.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(76676..77545)
                     /locus_tag="ONT67_000196"
     CDS             complement(76676..77545)
                     /locus_tag="ONT67_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2353965.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            77671..78351
                     /locus_tag="ONT67_000197"
     CDS             77671..78351
                     /locus_tag="ONT67_000197"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2353966.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            78504..79484
                     /gene="pta"
                     /locus_tag="ONT67_000198"
     CDS             78504..79484
                     /gene="pta"
                     /locus_tag="ONT67_000198"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR2353967.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            79607..80080
                     /gene="tsaE"
                     /locus_tag="ONT67_000199"
     CDS             79607..80080
                     /gene="tsaE"
                     /locus_tag="ONT67_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR2353968.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            80073..80597
                     /locus_tag="ONT67_000200"
     CDS             80073..80597
                     /locus_tag="ONT67_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353969.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(81087..81620)
                     /locus_tag="ONT67_000201"
     CDS             complement(81087..81620)
                     /locus_tag="ONT67_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR2353970.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(82484..83236)
                     /gene="xth"
                     /locus_tag="ONT67_000202"
     CDS             complement(82484..83236)
                     /gene="xth"
                     /locus_tag="ONT67_000202"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR2353971.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            83349..84269
                     /locus_tag="ONT67_000203"
     CDS             83349..84269
                     /locus_tag="ONT67_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2353972.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            84342..85277
                     /gene="murB"
                     /locus_tag="ONT67_000204"
     CDS             84342..85277
                     /gene="murB"
                     /locus_tag="ONT67_000204"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR2353973.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            85575..87053
                     /locus_tag="ONT67_000205"
     CDS             85575..87053
                     /locus_tag="ONT67_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2353974.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            87330..89390
                     /locus_tag="ONT67_000206"
     CDS             87330..89390
                     /locus_tag="ONT67_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCR2353975.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            89473..90042
                     /locus_tag="ONT67_000207"
     CDS             89473..90042
                     /locus_tag="ONT67_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2353976.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(90217..91023)
                     /locus_tag="ONT67_000208"
     CDS             complement(90217..91023)
                     /locus_tag="ONT67_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2353977.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            91161..92675
                     /locus_tag="ONT67_000209"
     CDS             91161..92675
                     /locus_tag="ONT67_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCR2353978.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(93078..94061)
                     /locus_tag="ONT67_000210"
     CDS             complement(93078..94061)
                     /locus_tag="ONT67_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCR2353979.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(94066..94713)
                     /locus_tag="ONT67_000211"
     CDS             complement(94066..94713)
                     /locus_tag="ONT67_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2353980.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(94798..95430)
                     /locus_tag="ONT67_000212"
     CDS             complement(94798..95430)
                     /locus_tag="ONT67_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2353981.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(95446..95997)
                     /gene="coaC"
                     /locus_tag="ONT67_000213"
     CDS             complement(95446..95997)
                     /gene="coaC"
                     /locus_tag="ONT67_000213"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCR2353982.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(95994..96731)
                     /locus_tag="ONT67_000214"
     CDS             complement(95994..96731)
                     /locus_tag="ONT67_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCR2353983.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(96728..97717)
                     /locus_tag="ONT67_000215"
     CDS             complement(96728..97717)
                     /locus_tag="ONT67_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2353984.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            97975..98517
                     /locus_tag="ONT67_000216"
     CDS             97975..98517
                     /locus_tag="ONT67_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2353985.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            98534..99619
                     /locus_tag="ONT67_000217"
     CDS             98534..99619
                     /locus_tag="ONT67_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2353986.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            99631..100437
                     /locus_tag="ONT67_000218"
     CDS             99631..100437
                     /locus_tag="ONT67_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353987.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            100438..101265
                     /locus_tag="ONT67_000219"
     CDS             100438..101265
                     /locus_tag="ONT67_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2353988.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            101262..102335
                     /locus_tag="ONT67_000220"
     CDS             101262..102335
                     /locus_tag="ONT67_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2353989.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            102763..105459
                     /locus_tag="ONT67_000221"
     CDS             102763..105459
                     /locus_tag="ONT67_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2353990.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            105536..106024
                     /locus_tag="ONT67_000222"
     CDS             105536..106024
                     /locus_tag="ONT67_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2353991.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            106036..106530
                     /locus_tag="ONT67_000223"
     CDS             106036..106530
                     /locus_tag="ONT67_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2353992.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            106551..107360
                     /locus_tag="ONT67_000224"
     CDS             106551..107360
                     /locus_tag="ONT67_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2353993.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            107357..108178
                     /locus_tag="ONT67_000225"
     CDS             107357..108178
                     /locus_tag="ONT67_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2353994.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            108229..110328
                     /locus_tag="ONT67_000226"
     CDS             108229..110328
                     /locus_tag="ONT67_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCR2353995.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            110325..111443
                     /locus_tag="ONT67_000227"
     CDS             110325..111443
                     /locus_tag="ONT67_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2353996.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            111482..112360
                     /locus_tag="ONT67_000228"
     CDS             111482..112360
                     /locus_tag="ONT67_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2353997.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            112551..112703
                     /locus_tag="ONT67_000229"
     CDS             112551..112703
                     /locus_tag="ONT67_000229"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2353998.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVLVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        37233 a        19310 c        23446 g        33056 t
ORIGIN      
        1 gttcttttag cagatgaatc cttagaggaa tatgacacag taagcaaaca aacaatgttg
       61 tatcgtgaat tactttcaga tgaagaatca aaagaagatc tgattgccac aatcgttgat
      121 attttgggat atgcaattgc accagagtat cttttcaacg tcttagcgga tcaagcaaaa
      181 caagcaacgt tccaattgaa cgatctaaac aaagcctttg tgcaattggc atctacctac
      241 aatcaattta atgggttatt tgatgatgtg gatctccaat caaaaaaact aggaacagat
      301 gagcaacaac gaaatgtgac gattaccgaa gtcatcaaga aattgaatga tgtagatgtt
      361 ttagaacacg atggggacgt gatcggggat gcgtatgagt ttctgatcag tcaatttgct
      421 tcagaagcag ggaagaaagc cggagaattt tacacacctc atatggtttc tgatatgatg
      481 gcgcaaatcg taacgctcga tcaaaaggaa cgacgattct ttagtgtttt tgatccaacg
      541 atgggttcag gttcattgat gttaaacgta cgtaactact taacacaccc agataacgtt
      601 aaatatcatg ggcaagaact aaatacaacg acatacaatc tagcaaaaat gaacttgatt
      661 ttacatggtg tcgacgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggac
      721 tggccaacag atgaacctta cacatttgat gcagtagtaa tgaacccacc ttattcggct
      781 aattggtcag cagatacgac tttcttagat gattctcggt ttaatcgtta tgggaaatta
      841 gcgccaaaat ccaaagcaga ttttgcgttt cttcttcatg ggttctatca tttgaaggaa
      901 acaggtacca tggccatcgt cttaccacac ggagttttat tccgtggcgc tgcagaaggg
      961 gtcatccgtc aaaaattact agaagatggc agtatttatg ctgtgatcgg tatgcctgcg
     1021 aacttgttct ttgggacatc gattccaacg acggtgattg tgttgaagaa gaatcgtcaa
     1081 actcgagatg tattattcat tgatgctagt cgtgaatttg ttaaagggaa aaatcaaaat
     1141 aaactatcgg aagaaaacat tcaaaaaatc ctcgaaacct acgctgaaag aaaagatgtc
     1201 gagaaatacg ctcatttggc aacctttgat gaaatcaaag aaaatgacta taacttaaat
     1261 atcccacgct atgtagatac tttcgaagaa gaagaaccaa ttgatatggt tcatgtcgga
     1321 aatgacatca agaagatacg acaagaacag caagtgctag aaaaaaaact tttagaagct
     1381 atctcttccc tgcaaacaac acctgaaaat gaagcgtggt tacaaggtgc gctggaggtg
     1441 ttcaaacatg agcaatgata cacaaccaga aatccgtttc ccaggattta cagatgattg
     1501 ggaacagagg aagttggggg acttcgttgt agattatgtt gaaaagacaa gtgtacaaaa
     1561 tcaatttcct atgctaactt catctcaaca aaaagggata gttttgcaag aagactattt
     1621 tgcaaatcgg caagtaacta cagaaaataa tattggttac tttgtactac ctagaggata
     1681 tttcacgttc cgtagtcgaa gtgataatga tgtgtttgtg tttaaccgta atgacattat
     1741 tgatagaggg attataagct atttttatcc tgtatttact ttaaaatctg ctgattccga
     1801 cttcttctta agacgtatta acaatggaat ccaacgacaa ttatctattc aagcagaggg
     1861 aacaggtcaa catgttctgt ctctaaaaaa gtttaaaaat atagtagcaa tgtttcctag
     1921 tgaggaagaa caacaaaaaa tcggcacctt cttcaaacaa cttgacgaca cgatcgctct
     1981 tcatcagcgt aagttagatt tgctgaagga aacgaaaaaa ggattcttac aaaaaatgtt
     2041 ccctgaaaac ggagcaaaag tgccagaaat ccggtttcct ggttttacgg aagattggga
     2101 agagcgtaag ttgggagaat taggtaaaac tcaatcggga attggatttc ctgatgccga
     2161 gcaaggtggt tctgaaggta ctccattttt taaagtttcc gacatgaata acattggtaa
     2221 tgagtatgaa atgagaaatg caaatcatta tgtatctaat gagcaaattg agcgtaaaaa
     2281 atggaagcca ataaaagatg tgccagcgat tatatttgct aaagttggcg cagcaataat
     2341 gcttaataga aaacgtttgg tcacaagccc tttcttaata gataacaata ccatggctta
     2401 tttatttaat aatacttggg atatctattt tggtaaaata ttgtttgaaa ctataaattt
     2461 gccaagatac tcccaagtag gggcattgcc ttcttataac agttctgata ttgagaatat
     2521 cagtgttaag gttcctgtaa aggatgaaca acaaaaaatc ggtactttct tcaaacagct
     2581 agatgatacg atcgctcttc atcaacgtaa gttagattta ctaaaagaaa cgaaaaaaag
     2641 cttcttacaa aagatgtttg tctaggaggg aagaagaatg aaaattagtc accgtgagga
     2701 agcagaagtc gaagaacagt tgattcgtgt acttggagaa gggcataatc agtggacgta
     2761 tcgtccggat ctaaaatcag aagaagacct ttgggtcaat ttacgccaaa aaattatctc
     2821 aaataatcaa gcagaactga atgattttcc tttgaccgat aaagaatttg aaacgatcaa
     2881 aacggaatta ctgctgcgaa cgaaaacccc atttgatgct gcgaaatggt taaaaggtga
     2941 aaatgggatg gctcggatca cgattgaacg ggaagattcg caattgggtt ctgtctcact
     3001 tatcctttat tccaatcaag acattggtgg aggaatctct acttatgaag tggtccatca
     3061 aattgcaaaa cgtggaagta acatagaagc acgggatcga cgttttgacg tcaccttatt
     3121 aatcaatgga ttaccgattg tccaaatcga gctgaaacaa gtaactgcaa aagatggtgt
     3181 ttatcaggca tttaaccaaa tcaaaaaata tgcagaagaa ggtatgtttc ggaacaatat
     3241 cttttcgacc ttacagttgt ttgtggtttc caatgaacaa accactcggt attttgcaaa
     3301 tgccttaccg aaagacttgc accctaagtt tttatttagt tggcgaacga aagacaatga
     3361 aaaagtagaa aacttatatg agttttgtaa acaagtgtta aatatcccgg atgctcatcg
     3421 tttgattgcg gattatacaa tcgtcagtga agatcaagac aataaaaccc tcatggtgtt
     3481 acatccttat caagtacatg cgattcaagc cttgtttatt gccgcaaata aacatcaatc
     3541 agggtatgtg tggcatgcga ctggttcagg aaagacgttg actagttttg tatctactaa
     3601 gcttttagca cgaaaatcgg ggatcgatcg aacgatcatg ttagtggacc gcaaagattt
     3661 agataatcaa accaccacag agtttacaaa atttgcttcg gaatttaata ctgggatttc
     3721 ttccgggaac gccaaagcga atagcttgat cgttggaacg ggaagtgcca aagaattaag
     3781 tgaaaccctg ttggcagatg ccaatgcaaa tgttgtaatc attacaaccc gtcaaaaact
     3841 agatgcggcg ttgaaatatg caaagaaaca agaagagaaa aaaggaacca atcgcttcca
     3901 aaaattaatg ggccaacata tcgtgtttgt agtagatgag tgtcatcgag cgttaagtgc
     3961 tgaaaatatg gaagaaatca aaaaaatgtt tccgaaatcc acttggtttg gctttacagg
     4021 tacaccgatt tttcctgaga atcggaaaca agccaaaggg caattggcac gtacaaccca
     4081 tgatcagtat ggagaggtct tgcataccta taccattaag aatgcgctag aagatggctc
     4141 tgtcttagga tttcaagtag agcatgaaaa caccgtggag ccgacttcac tagagaataa
     4201 aatctatcgg aaattaaaag aagtagaaac atatgcagag tattcatcag aacagatcaa
     4261 tcgtatgatt gatcaaatgg aacctgtcaa aaaagaaagt tatttggacc cagctgtttt
     4321 tgaagcagat gaacatatcc aaaaagtcat tcataaaatg ttccgtccag ataatgccta
     4381 tacaaaattt gattttcgaa atggcaggcc aacgaaatct gcaatcttaa ccacgagttc
     4441 cattgacatg gccaaaaagt attatcgggc catcaaagag atgacgaaag agcctgattg
     4501 gttaacaaaa gagtttccca atcagctcat tcgtgaagga cgaacgatgg aggatcctga
     4561 ttttccacga atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca
     4621 acaagaagaa atgcaaaaaa ttattgagga atataatcag tattatgata cttcctggtc
     4681 attagcagac attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc
     4741 ggaattcaaa caatttggga aacaaatcga tttagcgatt gtagtggatc gtctattaac
     4801 aggttttgac gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg
     4861 attgatccaa gctttttcac gtacgaatcg tacgtatcct gataagacaa aaggattgat
     4921 tgtgacgttt cgtaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta
     4981 ttcaaaagca aaagaagaat caggtttgat ttatccaagt tatcaggaat ccaaaaaaag
     5041 attcaaacaa gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca
     5101 tacgccactt gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgctta
     5161 tgaatcgtta gtgacttatg acgattacaa tgatgacatg gtgaaatctc caactttgaa
     5221 caatcaaatt caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt
     5281 aatagaggaa gaaccagaaa aagaaggaga agatttttca acgatcgaat tttatagtga
     5341 aaataccact aagttgtatg atattgattc aacttatatt gatcaattac taggaactta
     5401 tgctgcgaat agttcagatg cacgagaaga aattgaaaaa gctttggcta aattgaataa
     5461 aacagaagga gtcaaagaag tttaccgaca aattttaaat gcatttgatg tggggacttt
     5521 agatagtaat gaagatatct ttgtcattaa acgtcgctat tttacacaag cgagtgatga
     5581 tttgattcat gtcttttcca atgagtggtt tgtttctgag aaagaattac atgcttctaa
     5641 tctgcaatat ctaccaggtg aagatccaat tcctaatatg aaagcaatta tcaacagtaa
     5701 ggattacgaa ggatataaag ccaaacatcc agaagcgaag ccatttaaat acccacaaga
     5761 aatgaagcga gcatggcgta aaatgttaga tgatgagttg attccacttg agaatgaatt
     5821 gaggtaaacg agaagagtta agaaaattgt acgattcaaa gatggtagtc aagtggaagt
     5881 gtagaaaata gggatagatg tcttttattt gttcacattt tataacgaag tatttaagac
     5941 agctattgaa taatgtttcc tccacctttt tcattcattt accccacccc gttgttatat
     6001 gaccgtgttt tgagaggaac caataaaaag cataggctaa ctaggggttc ctttttcttt
     6061 tgtttttttg gaatccgctt aagtgaaatg tatctgaaat gaaacggaga taaagtgatt
     6121 ttttgctata atgacaaggt tttagctgtt tcagatacca tgtaacccaa aatagtttat
     6181 tgagaaaata ggagagtatt tgatactaac tagtaggaaa aaatgagata aaaattaaat
     6241 ggaatcattt caggtaatct caaataaaaa aatatacagt cctgtggcac tttacctatg
     6301 ggactgtata ttttttactc tcagcgggct tgttttgact ccgtgtctga ctccgtcgta
     6361 ttgattttaa atgatattcg atgaccattt aacaggtcat ttttgaaatt tttgtctcta
     6421 tatgactcag tttgataatt attgatagat caatttgggt ctatatcgaa caagggaata
     6481 atccaataaa aaatgattta gaatatcaaa ataacaaaaa agaggactac ctgtattcgt
     6541 cctctttttt gaccagattt tgaccagtct aatttacatt ctaatatatt catatgagct
     6601 aattttttga ttaaatggta agtttttcct tgtttgtttt agtttaaact agtgtgtaat
     6661 ttaaaaatct atatggaagg gtctgtgggg taatacttaa ataaaggcta ggaacctgat
     6721 aacatcaagg ttcctagcct ttttataata aaatgaagcg aaaataattt cttttaaaga
     6781 gccgagatta tcagctatat aagagtgaat ttgatgcaaa aaacagagcc gaaacagtct
     6841 tggtgttcgt agctctgctt atatgtttcg aatttaagac aaagagttgg caaaagatgt
     6901 tgataaggaa gataagataa ctgtgttata taaactaggt aactaactgt aacaagctaa
     6961 ttttaagaga ttcttattag aagacgttta ggaatataac taagaaaatg attatacagg
     7021 taattgtcat ttcaataatg ttttctaatt ttcgcgtctt tgttttaaga aaaaggccag
     7081 aatcagttgg aaaaaagttc gtacgatagt gaagaggggc aaaatttcct actaatattc
     7141 ccactaacaa gagataaaac ggccaggttg gtgtgattcc tctataaaaa aatgcccatg
     7201 ctaccgaaat cccatagatt aacaactgaa caatttttac aatgaaaaaa ttgttctgcc
     7261 attactcaaa gtctcttcct ccttgtatcg tttatttgtt tcattttatc attatgatag
     7321 gcgcttgaac aatccatttt gtattttatt gagtactctt ggataaacta ccagcgatta
     7381 tcatatttat ttatgttact gtaagccatc atataaaagt aaatgagcag atcatagaaa
     7441 tataaaaaag aactaaaatt gatctaaatt atgtgcagct tgactacgta ccaatctata
     7501 ttacttacta gattggtcgt taatctattc ggatcgaatg agcaaaatat ttttgtgaat
     7561 gtccatcctt gattatgatt ccaaaaagaa tcattgggag gctgtggcgt aaatattata
     7621 ctataagcca gaaataccta tcattctgaa cttttttatt taggaacttt catctcaagg
     7681 ctttttcaat taatttgtac ccctgttttt tggataagat catgtgcatt gattttttct
     7741 atctctacgt tcatctccta cttaatgcct atctggctcc tgagggcttc tttttaaggc
     7801 gtcgtcatag tcttggctga ggatttcttg gtaatctgag ggaatggctt cccaagtgag
     7861 caggcgaacg ttcatgacgt gttttccttt gtgtttgatt acagcatggt ttttctcttt
     7921 ttctacctct ccagcgtgaa aagacagcca tgtaccatgg ccaaagatat cattacatac
     7981 ccaacgttcg atggaattgt ccacgtatcc tacagggagt gtggtatagc cgatttgtgg
     8041 tttcaaaaaa ggattgaatc gatcgatcgt ttcatttttg tattgataat tgataagaaa
     8101 aagatagacc aaacaaaaga cgagcagaaa gaaagatagt gtctttttga gtgagaaggg
     8161 ttgttttgat gaataagatt tcttgttaac aagaggttca tctggcaatg ttggttctat
     8221 tgattcggta gctgattcaa tagaaggaaa atcaatcggc agacctaaag tatgatgtaa
     8281 aagtgacagc atttcaaggt ctggcattgt acgagaattt tcccaactag aaatcgtttt
     8341 atcggaaaca ccaatttttt cgcctaattc tttttgcgta agcttacaat ttctgcggta
     8401 agttcgtatt ttttctccgg tgttcatctt cttctccttt cgattctaca gtatatagat
     8461 ttattctaca tgacctatac acttgataga tacgtttttt tgatgaaaaa cggtatgatt
     8521 ttatcaaaga atcatttaag taagggagag tggtgataat caaaaaaaag ttcacagttt
     8581 tactcacaat catagtgacc ggtatcatga gtggttttgc cttggttagt ataaataaac
     8641 aggtactcta tcggacaaat gaatttttcc atcaaacaga tacttatact ccgattcgat
     8701 tgccacaaca ttttattggg caagaagaat atgagcagct aatcatggta tttgaaaatg
     8761 tctcatcaga aactgagctt tcttatatga aaaaaacagt aaatgaatcc aatgaagtag
     8821 atacatcttg gaacttcaag atacagccaa agcttgaaat gacattgtat tatcaagggg
     8881 atgtcaaatc ttctcacgag aaggtaccct attctaatac ctcctttcgc gtaacgtcca
     8941 ttgaagaatc gatcaaaata aaagattttg aaggtgaatt tttcttgatg acgagagaaa
     9001 aagaagccta tgaacggttc atttcactat tcactgatcg attcaatcaa gcatttcaca
     9061 ctgattatgc cactaatatt tttgaagact ttgaaaatag ctattcgtat tcggttattt
     9121 caaatacaga cgtatataat tatcgagtgt atttaactat tggattgatt cttttattcc
     9181 ttttgttatc atcctttttc ttattgatga ataaagaaat cagtttattg ttgaataatg
     9241 acttttcatt acatcaagta ttgtggtgtt tattggggaa aaagaaaatc attattacat
     9301 tcataattgc aggaatcatt ggtggtttta tttttatgta ttataaggag caatttcttt
     9361 atttgttcca gatgtacgtt ttcttgttca gcggaatata tggtatctct ttgatttctc
     9421 ttttattagt tgaaaaaatc gcttcgttaa gagaagcgtt caactatatc caatacattc
     9481 tttttacatt tggattgttg ttttctcttc ttttgaccgt tgccaatgtc gaattagcaa
     9541 ctatcgtttt ttcttcttca cagttagctg tttcccatga accaccaaaa gaaatcgctg
     9601 gtgaggacta tcatgtattt tatccagtaa ccatcgggaa aaaccacgtg gattttattt
     9661 attccaatcg ttttgcatca gcagcagatc aggaattata tgaaactttg aataaaaacg
     9721 gaagtatatt agtcaatgta tcagattact tgaatgaaga aaatgaacag tttggcagag
     9781 ggataaaaat aaatctgaat tatcttaaga aatttccttt gattgatgtc tctggaaggt
     9841 tgatccaaat aaccgaaaaa gaaacgcacc ctgttatatt gatccctgaa cgatatagac
     9901 atacagattt aaaaaatgac ctggcgcaaa taacagaata ttatcaagaa atcagtgaaa
     9961 aagaaccgaa atttctattt ataaaaaaca atcaacccat ttatacattt attccttcta
    10021 tcccctggat cgaacattat ccggttgttg aaatactgac attgaaaaac agtatgtatt
    10081 tggaaagaaa tattctcagc ggagaaatct atccaccgtt aaagatcaag ataggctctt
    10141 tgtcgaaaga aaggttattt gagctgattg aagaaagtca gttacgtgat aacttgcagt
    10201 tatcatttcc gtatactcag actgaagaga tagctgtcaa agtgttaagc agatcatttc
    10261 attacttgat acagatattt ttactcactt tcttttcttt cttcttgttg agctatgtca
    10321 tgttatgcat atattttgtc tacaattgtc ggaaaatcgc tatctttcgt tcaagtggtt
    10381 attcgctctt tgaaacgtat aaagatttct tcatgatgaa cctcatcaaa tggggaacga
    10441 caagtgtaat cttccttttt cttatagaaa gagagcctaa atatcttttt aatatttttt
    10501 tctttagctt cattgatttt attctttcag ttgtttttat tttgtctaca gaaaagaaaa
    10561 gtcagttatt gctgatgaat ggaggttaac aattggagcc gttgatagtg ataaaaggtg
    10621 cgaaaaaaat cattcgaaac aaaacgattc ttcatttgga tcgtttcact gcggatgctg
    10681 gggatcttgt gactgttgtt ggagaaagtg gagcaggtaa aagtacgtta ttgaatatat
    10741 taggattgaa tgatcagttc agttcaggac aattctattt gttcgatcaa gagacgaaac
    10801 aattaactgg gagagagaag cgcttgataa aaagaaatac catatctttt ctttttcaag
    10861 attttgggtt gattgaatat gaaacaatcg attttaattt agatattgga ttgaagtttt
    10921 cgaatttaaa tgtttcagat aagcgaacag caaagtatga ggcattgaaa aaagtaaata
    10981 taaacaagaa gctaagtacg cctatctaca cattatctgg tggtgagaag caaagaatcg
    11041 cctgtgcgag agtcttattg aagaatagca aaatcatttt agccgatgaa ccgactgggt
    11101 cattggataa aagaaataag gataaagtag ttgaaatctt atcgcaacaa gcttcccaag
    11161 gtacgttagt tatcgtagtc acgcatgatc catatctgac caaaatagga aacaagaaga
    11221 tcatgcttta aaaatcaaaa aggaaaggat gtcgttttat gaaaaaagca gttattattt
    11281 gtgcactagg gataggtttt gttggaggaa acgctatgtc atctgaagtg gaagcaaatt
    11341 cagttcacgg tgaaatctat tacaaatctt taaagccagg agagattcat tttgatgcta
    11401 aaggtaatat tattaagtca aatttaccag tagaccggga aatttctaga acagcaatta
    11461 atcatagctc tggaagatgg gtttattata gtgatacaac ttgtacgaga aaaacaggtc
    11521 attctaacca ctatagtcgt gtctataatc gtcacggtgc aaaagcgaaa gttggaaact
    11581 ctattcgatc agcaaatgca acaaaaggag tatgggccta tgcaagtgcg acaggaaata
    11641 gaactgacac attctcatgt tggtacaatc caaccggttg gtattaagta tccaaaagtt
    11701 tatataaaat agaaaaaaca tataatgaaa cagcgttaaa agttattttt ggtagtttta
    11761 atatattcat ttttagttgt ataaaaatat atcataataa aaacaacaaa aaaatgtctg
    11821 gaaaatcgaa cataaaagtg tctgatttga atatcccagc agcggaagaa gaaaaacaaa
    11881 atcgataaca tttgttctaa gaattagtta cgttaaatac ggaacgcaag atagacttga
    11941 aaaacggtaa caaaagaagt agtatattaa ggtgtgatgt gctacttgac atttattctg
    12001 ctgagcgaac tttctggatt gtgtagcctt atatctattc aggacgtatc gagcagatta
    12061 gtaaaggtgt tcttgatgat tttcatcttg aacacctttg tttttagcaa ttcgcaaaaa
    12121 acgaaaagta ggagaattga taaatgaaaa aaagattttt agcacttgcc atcgtactag
    12181 gcactggctt attgtcaggc tgtacgaatg ccggtgaaaa aacagccgtt agttataaag
    12241 gcggtacaat cagcgaacaa gaagtaatgg atagcttgaa aaaaatgcaa ggagcagatt
    12301 ccgctgtgca acagttgatc gtttatcaag tatttgaaga taaatatgga gatgacgtat
    12361 caaccaaaga aatcgattct caatatgatc aaacaaagaa acaattgggt gattcatttg
    12421 attcacaatt gaaatctgca ggttacacag aacaaacatt caaagacagt atcaaacaaa
    12481 gtctagcctt tcaagaaggt ctaaagaaac atatcaaatt gaccgatgaa gatttaaaaa
    12541 cggcttggga atctttccat ccagaagtgg aagctcaaat tatccaagta gcaagtgaag
    12601 atgatgcaaa agatgtgaag aaagctgctg ataagggaga cgatttctct aaattagcca
    12661 aagacaagtc aactgatact acgacaaaag aagatggcgg aaaagtgaaa tttgattcca
    12721 ctactacaac ggttccggca gaagtcaaag aagcagcctt taagttgaaa gatggtcaag
    12781 tttctgatgt gatcacttct accaatgctt caacttatac tacggaatat tatgtggtta
    12841 aaatggtgaa aaaccaaaac aaaggcaacg acatggataa atacaaaaaa gaattgaaag
    12901 aaattgcaac agacacaaaa ttaagtgaca gtaccttcca gaataaagta attggcgaag
    12961 tattgaaaga cgctaatgta aaaatcaaag acaaagactt tgaaaatgtt ttgagtacat
    13021 tcacttcaga ttctagtaca gcaagtagta aataagcggt ataaataaaa aatatgaact
    13081 atttaaatca ataaaactgt tcatctaaat ctttttatta gaactaaatc aacagtagtt
    13141 taattactac ttgatttagt tctttttatt ttaaaaattt tttttactta gaatccagcg
    13201 accctagata tttttctgta taatcttaaa taaagaaaac aatatcaaaa aatgcacgta
    13261 gaagtagata aatggagtgg ttttgcaaat ttcttttatt tctagaagtt attcacgtat
    13321 acggatttgt ttttctcaca aataaaaagg caggaagtac aatggatcat ttgtatataa
    13381 aatatattca tcaattttcc gttttggtgt taggcacact aattatagcg gggattgtcg
    13441 catttattat tcaaggccgt gacatattaa cagtacatca agagtttttc agttatgcat
    13501 ttggtattcc tctgatcgct ataaggtctg ataacaaaaa gtggaagaaa cgctattaca
    13561 ttctcttgct atttttgtgt ttttatctgc cacctaagat ttttacttta ttaggtatac
    13621 aatgcctatc attttctttt atttcctata tcattgtttt aattttcctc agtttcatag
    13681 tatggatgtt ttatcttgtc aaaaaataaa tgaatgtttc atgaacagta cttcatcaca
    13741 gcctaatttt tttctttatg ctatcgttat attaaagaaa tcaatcagaa gggaaagaaa
    13801 gtgatgaaaa aagttttatt agtggtaaac cccagttcag gcggtgaaca agcaaaggaa
    13861 tttgagcaat tagcgatagc aaaattggaa tcggtgtttg atgaagtggt cgtcttgcat
    13921 acaaaaaaag caggggatgc aaaaaacttt actcgcgaag cggctacgga agggtatcat
    13981 agtgtgtttg tcatgggcgg agatggaaca gtcaacgaag gaatcagtgg aatcgctgaa
    14041 caagaacatc ggccaaattt cggatttttc ccattgggta cggtgaatga cttggctcga
    14101 gcattaggga taccgctaga acctgaggaa gcgatcaatc atttttcgat tgaatcagtc
    14161 aagccattgg atattggaaa aataaatgac gactatttta tgaatgtcgt cgcaattggc
    14221 tctatccctg aatcaatcaa tgatgtagat gcggagaaga agacgaaatt tggaaaacta
    14281 gcttatttca tgtcaggaat caaacagtta gcttccacac aatcctactc gtttcacgta
    14341 gaagttgatg gaaagaagga agaaattgag agcagtacct tgctgatagg actaactaac
    14401 tcagtcggcg gttttgaaac attacttcca aatgcaaaag tggatgatgg taagctccat
    14461 ttggtttatt taaaagattc ttctttgatc gatacgctta aaacattgcc agacctatta
    14521 aaaggagtag atgaatctac gaaaaatcta gcctacctta cttgtgagca attagttgtg
    14581 tcattagctg atgaggaaga attgacgacg aatgttgatg gagatgaagg ggataaatta
    14641 cctgtgacaa tcaaagtatt gccctcacat ttacaggttt attgttaaaa tggaggttgt
    14701 gaccgaggag ttggcgggag aacaccgctt gttcgggata agggactgtg acaagacttt
    14761 tgtcacagcc ttttttttgt acaaaaaaga aatttacacc taatagaaaa atttttccat
    14821 aaaaatacat attttaaata tgaaagtaca attggaaata gattaaaacg ataaaataaa
    14881 attgtcaaaa cgtcagagag gatggtggag aatgaatcaa tgtgtaaaaa agctgattgc
    14941 gtcgattttg tttgtcttcc ctctcttgat ggtgacatcc gtttttgccg atgtagagaa
    15001 aacaaacgaa tcaacgcaat cagagaatca aagttttgac aagactactt tttcagaaga
    15061 atcgattact tctgaaacta cagaatctca aagtgaggct tctactaata aaaggcaaga
    15121 agaaaataag acaagtgaga cctcagaagt agctgaacaa acgtctgata gcagtgaagc
    15181 ggcactggca agagctgttt cagcggatgg gtacagcgaa gctgccacaa cagaagaact
    15241 ggcgcagctt ctcgcagacg aatcagtaac taagatccga ctgattcagc ctttgacttt
    15301 agacagggaa ttagagatca aacgagatat cgttattgat tttggtggat tcgctcacaa
    15361 tttcggcaca caccatattt atatcaatga gatacctcag tcgatcgagt tccagaattt
    15421 taaaggaaca gctgcacatc caggtggatt gacaccaaat atcgatggaa atgccattat
    15481 cattgcttat atgaccgatt tttggggaag cagatatcat tttacgggag aaataaaatt
    15541 taccgggaca cttgatttgt atgatgggtc caaattagga ctgatttacg ctcctcgtgc
    15601 aactgtcaca ttagatggtg tttccggtgt gttggatgtc caaccagtga aggaaggact
    15661 gaatgccgct ccgggtaaag cctatttttg tagaagctat caattgaatg tgatcaatgg
    15721 tagtcaactt tatggaccgt atctagggaa attctacggt tttctagatt caggagatga
    15781 gactggtagt ggaaaaacag cgccaggaat ccatatctta agcggttcaa aggtctctct
    15841 tgattatgat cgggcagatg gcgttagcga tgaaggagag gcagtcgata cgctacctgg
    15901 taatgtcatg tttgaagtca gtggttcggg aagcgaattt tcggtaaata caaagatcaa
    15961 tattaccaat gacaacaatc gcggaatcat ccaaatgaga ggttcaggat cgaaagtcac
    16021 tgtatcgaat ggtgggaaaa tcaccatcaa tacagcaact acgggcggat ttcgcttgca
    16081 aggagattct agtcaaatcc atgtatcaag tggtggacaa atctcggtca aaatggctgg
    16141 agatgacgat caacccggga acaatgggat gcgttttgta ggaaaaggcc tttctttgat
    16201 cgtagaagga gccggatcag ttatcgacat cgataagcag agtgggcgtt ccagcgcagt
    16261 tcgttttgag aatggtacgc aaaagctgac agtaaccaat ggaggctcat tgagggtaag
    16321 aaatacaggt gatggcaaat cttacgtgaa cagactcaat caaggaaatc aagcgatcca
    16381 attttatgac gcttctggtt ttctagaaag tccaggttca gctgatttta cagtcactgg
    16441 agaaaaatca aatatcgata tacgcgcaga ttccggagca actgtagata caactggaga
    16501 tattgattta aattttacgg caggtgaaaa aacttattta gtcatggtcg ggaaaacagg
    16561 ggaagataag accggcatca tcagtgggga tgtgttgaat gtggacttag aatctcctac
    16621 ctattttgat ttccaaaaca aacggattgg aagcgagacc aatggcatag gcggctgggt
    16681 attccaagga aatggagatt cttctttaaa gatcgcccat tcagaaatcg ctttatggcg
    16741 aaaaacaggc gcaacattcg ataactttga aaaagacccg gattattttt ctgatatgac
    16801 agacttatcg atcaatggga ccgacttagg caatttcgta tcaagcccta gtgcaggttt
    16861 aacaaatgaa tttgctcaga caggtgccgg aatgaaaaat tataatcgaa tcagtgccaa
    16921 taatcagctc ccgatgattg aatccttaag agtgccaacg aatgcagata aaaaaatcta
    16981 tgggcatgtc gttgttcccg aaggtgtcga ggcaaagcca agagatgctt ggacaggcga
    17041 agtagaagtg actttacgta ttacttacgc agatacgacg aaatcatctg tagaaattac
    17101 tgctctcacg caaggacaaa cagatgaaaa tgaaggggca ggatataacc catggggaga
    17161 aggcgaacaa ggcggcttgt tcgaagttca agtacccaat gatgagagat taaaaacgga
    17221 ggatcaagtg caagttgttg ctgctaaaaa agtaagaggt ggccaagaag tgacagaact
    17281 ggactctgcc aaaacgacag tcgatgtgat tcctcctgaa cctgctaatt taagtaccac
    17341 attgatcaat gcagcttctc gcacgatcac aggtacaggt gaagcaggag ccttggtttc
    17401 attgaaaaaa aatcaagctg ttttgtcaga aacaactatt gatgatacag gggaatttgt
    17461 tctttctatc ccagaaaatc aactttttgc aggagatgta ttagaaattg tattaaacga
    17521 ccaagcagga gaagctactg ctaagggtgt gcagaacaag ccaagtacca acgatgaaat
    17581 tgggaatcac aatccaagtg atacgccagt tgtttatcat gatgccccgc cttttgcacc
    17641 tgctacgaag atcgtagtag aaggtggtct ctcatttctt gccccttcca aactcgattt
    17701 tggtcagatc aaagcaactg gtttgacaca agaagcattt ggccaaatgg atatacagga
    17761 aaaattatgg atttatgatc agcgagaaac gaaaaatccg tggatcttaa cgataaaatt
    17821 atctcagcct ttcacaaatg atgcaggatt ttctatgcag gatgtcctat atttcaagaa
    17881 aggagaggag tatcaattag tgaatgaaga agaactggaa attgtcagag gacaaaattc
    17941 aacgaatgtg caagcagatt attcagaata tttaaataac ggtcgggcgt ttaaacttgt
    18001 cttaaccaaa gaaaatcaga tagctggtaa ttactcagca gagataacat ggacattagc
    18061 agatgcgccg acagaataaa gatcaaggag gatgattgat gaatcgaata tggaataaaa
    18121 gtcatcgaaa caactgctct ttacttatga tccttctcct tggattcatc ctttggcaga
    18181 cacctgtcca tgcctccgaa ttcaattttg cggtagattt tgaattaccg gctaatcaga
    18241 ttgataagaa caaaacatat ataaatgtaa ggatggagcc tgggaaaaca caggaaatcc
    18301 cctacacggt ctttaaccga acagataaat cagtaacagt tgagacattg atccactccg
    18361 ctacgacgaa ttcaaacggt gtgatcgaat atggagaatc aaaaagtaaa tttgatgaaa
    18421 cattaagtta tgatatgaaa aacttagcaa aaacagtggg agaaataact gttcctcctc
    18481 aaagtgcggt cactgagaca ctttcactga aagcgccaga aaaagagctg gctggtgtgg
    18541 tcgcaggagg gatcacgttt caagaaaaaa aagagaacgt ttctaacaaa agtacagttg
    18601 gtgtcgtcaa tcagttttct caagtgaaag caatcttgat ttgaaatagc gaaaaagagt
    18661 cttccccaga tttgaaatta aaaaaagctt atgcggatca aagcgatgga agaaatgtga
    18721 ttaaggcgaa tcttcaaaat ccaaatgctt cttatatgtt cggcttgact acatcggttg
    18781 aaatctacaa aaaagaaaca aaagagcctt acctttcaat aaaaaaagaa aaaatagatg
    18841 tagcacccaa tacaaatttt gatttacttg tcccattaga aggaaaaaaa ttagctccgg
    18901 gacattatca agcggttgtg aagggtgctt ggaaagaaaa acgctggaat ctccataccg
    18961 attttgaaat aactgatgat gcagcggacc aattgaatga aaaggatatt gatttaaaaa
    19021 atcagccaga aacatttgac tggcgatggc tgatacttgt tggagtcatt cttattggct
    19081 tatttgctgg attatttatt ttaaaagaaa gaaggaaaaa gaaatgaaaa aaacaattgc
    19141 attaagtacc gttctagctg gaagtctatt attatgtaca caaagtgttt ttgctgagaa
    19201 taagagctca gccactacag aaggaacgat ttctgtgacc aaaccagatg ctgcaaatcc
    19261 tgaagacgtt gttccagttc ctcctgttga tccaaatgat cctgatactc cttttacacc
    19321 tgacgaacca aatcagggaa catccggatt actgacgatt gaccaagctc cagatcttga
    19381 ttttggaaca atcaagttag gtacagcaaa aacagtatat gctgcattac aaagcggaac
    19441 ggacagtact ggagcacaaa cacaagtaca gaatttcgta caagtaacgg ataaaagcgg
    19501 aaattatgcc ggttggaaat tatcagtcaa acgaacagaa tttattggta caacatctga
    19561 tacgaataaa ctaactggtt caaaggtgaa attcaaaaac gccgtgattc gaaatagcgc
    19621 aactaatagc agtggaatgc catctaccgt ttctggaact ggcgctgaag gaatcgaaat
    19681 cccagcagat caggcagtag atctagtaac tgcaggagca aatgaaggga ttggcacatg
    19741 gatttattca ttaggagaga atgtggaaca agggagacaa tcagtggaat tatttgttcc
    19801 tgtatccaat tatgctgtag acaactacgt ttcaacattc acatggtcgt taacagatgc
    19861 tcctacagaa gaggaagcgt aaaatcatat caacgatttc agggagtgga gccgattcaa
    19921 ggcccactcc ctgaaatctg ctatgcttac gtctcagctc ttattcatca actcgaagca
    19981 gttttttgga agtcatggaa acgttccttt gtcttttcaa agatctttcg accaacgagt
    20041 aaggagtcac cgttgaaaaa ttctaacaca taatgagcag aatcaatttt ctgcaattgc
    20101 gataagttga tacagtaaga ttttaaaagt aaaaagtaag catacttttc taactctgtc
    20161 ttcaattgtt ttaaagaagt attcactaat ttatcttctg tagttgtttt gaccataact
    20221 tggtttttca aacctttgat actttcaata tataaaattt cccgaggata caggacgaga
    20281 cttccacctt tttgatcaat aaagatttta tcgacattta cgatctgctg ttctttccaa
    20341 ttgatctgtt ctagcacttg acgaatttgt tcagataaat tcctgtcttt tctgatatat
    20401 ccaatcggaa ggattttccg attgatgatc tctactgcaa aattcgtata agcggtgaaa
    20461 aaaacaatat tactatgtgc atttttttta cgtatttgtt cggcgacatc aatacctgtg
    20521 agtgattgct tcaagtcgat atcgataaag aaaacatcgt gattatcaat agtgcccaac
    20581 tggatcaact gattgatttg agagatagaa gtgagctgca cgatattctc gatatgatga
    20641 ttgaccaatt ctctgatgat ccattcgctg tgaaactggt tatcttctat gataaaatac
    20701 tttttcatat gcaggctcct attaaaaaaa tatgtgtaca ttttgaaaca aaacaatgga
    20761 ggaataagat ggtcgcaaca gtgacacttt atttgacctt tactttgatt ttagatttag
    20821 gtatctattt attcttttcg aaacaactgc tagtaaaaaa cagctttggt tgtatagctt
    20881 tgatggtggt gacactttta cttcctatcc ctaaagtgag ctttccttac ctagttggct
    20941 tattattgat gtttcttttt tattttacta cgaaaaacct gtttatcagc tgtttgatca
    21001 gtaatctttt ttttgctatc actggactca gcttttttct ggtttttgat ctgatggatt
    21061 atttaggaaa gagacatctt ggaacagcaa gtttttttgc actacttatc gcatttattt
    21121 tctatctatt agtaaaaagt atagacaaac agacgagatt tctcaacttg ctttttactt
    21181 atccaactga acactatttt ggaggtattt gtattttatt aagctggggc gtgatttact
    21241 tggtattggg aagtgttcac ttccgctcat ctaattatct gcttctatct cttttgtcga
    21301 tagtcatcag ttttttttct ttgttttttt ccattatgtt gatcagtttc caagtaaaag
    21361 aaagacaaca tcaggaatcg cttcaactgt atcaaataaa tgaagaatat taccatcatc
    21421 tagaggaatt caaacatgat tataaaagtc tactgttttc attaaaaatt actcttcagg
    21481 aaaataaaaa agaaacgttg gaatttttag aaatattaga agatcattct gcagctattt
    21541 ttgaaaaacc gcaaatacaa cagctaacaa atattgtatc cccagctgtc aggggtgtct
    21601 ttttaaaatt tctagaaaaa gcagaatcgg agaaaatccc gatccaagta gttattcctg
    21661 tcgaagtgaa aacaatcccg attgatctgg ttgtttttat tcgctgttta tctattttta
    21721 tttctaacgc attcgatcat cgtgtagccg atcagagtcc gattacgatc aatctgctgg
    21781 aagaaaaaga gggatcacgt tttagtatag cgaacaaggc aaatccaacg aatttgacgc
    21841 tatcggagat ggtcaaaaga ggaaaaacct ctaaaaaaaa tggcgggtta ggtctataca
    21901 gtgcgaaaaa aatgttggat gtttacgaaa atgcagaact aaaaatcgaa ttttcaaaac
    21961 agaatcacta tttttttgta gaaatgtatc tggcatctag aaagagcaaa tcagcctact
    22021 gacttactgt caaaggataa gtttgaggaa agtctacacg gagatagaga caacaacaca
    22081 gcatattcaa aagaaacaga agcgtgatga ccgttgtcac ccttctgttt cttttgctat
    22141 gtagttaaac aaagtagttg acacttccgc tacttagtat tagtacgaaa gtatacttca
    22201 tgatactaag tagataagaa gtgaaaagcg gaacccacga tcgtcaaaat gctaactgct
    22261 ttgcttacac cagataaagg gaagagcaat atcgcttatt ttaggcgtgg agaagcaagc
    22321 aaatccctac gatcacaaaa aaaatgctga cccagcagtt aagagagtta gaacaagatg
    22381 aaagtatcca tcgaaaagcc tataaccaag tcccgcctaa agtagagtac tcgctcaccg
    22441 aagaaggaaa atcattaaga gagattctag tagcgttgtc tgtttgggaa gaagaacgta
    22501 tcgaaaaaca aaaagcctca agggaagaag acacgctact gcatacacat ggtggctacc
    22561 taaattattg attttctatg acatttattt taaataaaag aaaacaggag tttttttaaa
    22621 agcatcatct attttttatg taaaaaaatg gtatgattaa tcaagtaaga agtgattgga
    22681 agaaggcaag caaatgaata gtatggaaaa agtcaaccag tttagagatg aacgtaattg
    22741 gcgacaattt cataacgaaa aagatttggc tatctctatt tctttggaag catctgaatt
    22801 gttagaactt tttcaatgga aaacgccgga agaagtaaaa gaaacacaat tagaacgaat
    22861 caaagaagaa ctggcagatg tattgatcta ttcatacatg atggcagaca accttcacct
    22921 ggatctagat gagatcattg aagaaaaatt gatcaaaaat aatttgaaat atcctgtaga
    22981 aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag aaatgtgtca ttcggctgtt
    23041 ggaaagagca aaagaaaaac agtaaaatga gtctggaggg agagaaaatg aaaaaaaaag
    23101 gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct cttgttatta gtaggactag
    23161 ctttgatttt caatgaacag atcaaggatt attttgtcag agagacagga gataaatatg
    23221 ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa cgataaagat gtcagttttg
    23281 actttgatgc ggtagaacca atgacgacag aaggggtcat gaggtcgcaa atgagaggaa
    23341 cagatctacc agtgattgct agtatcgcag tcccttcagt ttcaatcaat ttaccggttt
    23401 tcaaaggctt ggataatacg tcactacttt acggagcagg aacattatct ccagatcaag
    23461 aaatgggaaa aggaaattat gccttagcaa gccatcgtgc aacgaaccct gaattgctgt
    23521 ttacaccgct agaaaattta gagatgggtg ccaagatcta tttgacagat ttagaaaatg
    23581 tgtatacata caaaacattt tttaaagaaa aagttgctcc tacagatacc caattactga
    23641 atgaagtcga gggaaaagaa attgtcacac taattacttg tggcgacatg gacgccgtca
    23701 cgcgactagt tgtacaagga gaactggaaa gtgttacatc gataaaagat gcgacagatg
    23761 atatgagatc tgctttcaat ctagagacaa aaacatttta ggatactggg acataagtcg
    23821 ttacagatta aaaacagcca aaggatatga atcgataagg aaaaaccaat ttgctgaaaa
    23881 aaagagaaac gcaaagaggt gcatctctga tttggttgtt ttctagatag acttgtgttc
    23941 cagttttttt attttttata tgaaaaggct tgcataaata aaaattcgat ggtatgatga
    24001 ttttgaataa gaaatggatt gttactgtac ggcaggcaaa accagaattc agctaagaaa
    24061 cagtaggcga attctaggtt tttttatttt tcttgagagg gcgaagcaaa atgaaaattg
    24121 ataaaatttt gaataacaat gtcgtcattt cgaaaaatgg attcggggaa gaagtcgttt
    24181 gtatgggaaa aggactagct tttcaaaaaa agattgggga cgagatttcg ccagaagcgg
    24241 tccaaaaaga atttgtttta agagattctt ttgctaaaaa ccaattccag cagctgatgg
    24301 ctgatattcc ggcagaggag atcgaatggg tcaaacaagt agtcgaactg gctgaagata
    24361 aactaaaagt tgaattttcg cctaatattt atctgacatt gaccgatcat cttcattatg
    24421 cgatcacacg agcaaaagaa aatatccaac tgcctaatcc attattattt gaaacaaaaa
    24481 aattttatcc gaaagaatat caagcagcga aagaggctct tttgttcgtg aaacaaaaga
    24541 cgggtgtgga actttctgag gatgaagccg gttttattgc ctttcatttt gtgaatagcc
    24601 aacaaggaaa cgaagacatg caagtgacga tgaccgctac cacaatggta agagatatac
    24661 tgagtatcat cagtaaattc ttcggcatcg tgttcgatga agaatctttg aattatcagc
    24721 gtatcattac tcatctgcag ttttttgccc agcgttatct caaaggggaa ggatctgacg
    24781 agcaagatga atttctctac gcattagttc aaggaaagta tccaaaagcg tttcattgtg
    24841 tcgaacggat caatgagtat cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc
    24901 agatttattt aacgattcat attcaaagag tagtaagtga gaaaaaaagc atccaatcat
    24961 aaggattgtt actgtatggc aggcaaaacc tggattgaaa aaataggttc acgaatgttt
    25021 gtgactgttt ttttcaatct gggtttttat tttttaaata aaaaagaaag aaggaaaaaa
    25081 caatggataa tcaagcagtt ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg
    25141 tcaaaagttt agtacattgt gctacgcggt tgcgattcag attaaaagat gaatctttag
    25201 cagatacgca aaagttaaaa gaagacccgg acgtgatcca agtcgtacaa agtggcggtc
    25261 agtatcaagt agtgattggc agtaatgtag cagatgttta tcaatcgatc gtggatgagg
    25321 aaggtcttgc agtttcggag gatggaaaag aagagtccaa aaatattttg aatcgtctga
    25381 tcgatattat ctctagtata tttacacctt ttctaggggc gatggcagcc gccggggtat
    25441 tgaaaggatt cttatcattg gcaacggtga tgggctggct gacagctgat agcggtactt
    25501 atcaaatatt gtttgctgca gcagatggtg tctttacctt cttacctgtc atgcttgctt
    25561 ttacagcagc taaaaagttc aaagccaacc agtttttagc agtagcaatt gctatggcac
    25621 ttgtgtatcc agctatcaca gcagttgctg gtgcgggaga agctatcagt ttctttggga
    25681 tcccagtgat tctttcacca tctggttata cttcttctgt cataccaatc attctagccg
    25741 tatgggttca aagtaaatta gagccattcg taaaaaaagt gattccgcaa tttctgcaaa
    25801 tgattttagt tccgcttgtc gttttagtag tgatggttcc attaacattt cttgctttag
    25861 gaccaatcgg gacggttgcc ggtaatgctt taggcggtct cttcaactcg atttatggat
    25921 ttagtccaat cgtagcaggt ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg
    25981 gcatgcattg gggattcgta ccgatcatgt ttttgaatat cgaacaatat gggtttgatg
    26041 tattgatgcc aatgcttctt ccagccatct tggcacaagg tggggctgct ttagctgtag
    26101 cattgcgaac aaaagatacg aaacttcgag cattagggat ttcttctaca gtgacttcgt
    26161 tatttggaat cactgaacca actgtgtatg gtgtgacatt acctttgaag aaaccattca
    26221 ttgccgcttg tatttctgga ggtattggtg gagctattat cggattttcc ggagtaaaag
    26281 ctttctccag cagtttggtc agcttattga cgattccgac tttcattaat acggtggatg
    26341 gtgtagagtc aaatgtgaca gtagcagtaa tagctacagg aattgctttt gttcttgcat
    26401 tcgtaggtac attgatttta ggatttgacg aacaaacaca agacaatcaa ttagaaaaca
    26461 aacatgcgaa tgcaggaaaa ccgattactt cagcacggca taccttaaaa agtcctttaa
    26521 cgggtaaagt actgccattg agtgaagtac cagaccaagt tttttcttct ggcgtgatgg
    26581 gaaaaggtat cgctattgat ccagaagtag gagaactagt tgcacctgca gacggagaga
    26641 tcacgactat ttttccaact ggacatgctg tagggatcac aacgactgac ggagcggaga
    26701 ttttgattca tatcgggatg gatacagtag aattaaatgg gaatggcttt gagatcttag
    26761 tcaaacaagg ggatctcgtc aaagcaggag atttattgat tcgttttgac atcgaagcaa
    26821 ttagagcagc tggctatagc gtaatcactc cagttgtcat tacgaatact gatgcatttg
    26881 ccgatatact tgaattagac caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg
    26941 taaaataagg aggaaatcag gatgcaatca cgtttttcag aaaaattttt atggggtgga
    27001 gcaactgctg ccaaatcaat tagaaggggc ttatgatcga gacggaaaag gactatccgt
    27061 tgcagatgct atgcctggag gtaagcagcg ctttgcgatt atcggcagtg aagaatttga
    27121 ctggacgatt gatcaagaaa aatatattta cccgaatcat cgaggaatcg atcattacga
    27181 tcgtttcaaa gaagatcttg ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc
    27241 gattgcctgg acacgaattt ttccaaatgg agacgaaggt actcctaacg aagcgggatt
    27301 agaattttat gatcagctga ttgatgagtg cttaaaatat gatattgaac ctgtgatcac
    27361 gatttctcat tacgaaatgc ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg
    27421 aaaactgatc gacttctatg aacgatttgc tcagacagta ttagagagat acagcagtaa
    27481 agtcaaatat tggatgactt tcaatgaaat caattcggcc tttcactttc cagctttaag
    27541 tcaaggatta gtaaaaagca atggagcagg agagtaccaa aatattttcc aagcatggca
    27601 taatcaattc gttgcaagta gcaaagccgt aaaaatcggc cacgaattgc gttcggatat
    27661 ccaaatcggc tgcatgatta tctatgcaac aacttacagc atcgatgcca atccagtcaa
    27721 tcaggcagca acaatgatcc aaaaccaaga attcaatttt ttctgcacgg atgtccaagt
    27781 acgtggagaa taccctgcat acacagcgcg tacccataaa aaatatggag tcgatccgga
    27841 aaaattagag caaacggaaa aagacttcca gttgctaaaa gaatatccag tggactacat
    27901 cggatttagt tattatatgt ctacggcaat caatgagact gatccacaag cagcaacttc
    27961 agaagggaac ttgctgggag gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg
    28021 gcagatcgat ccagaagggc tgagaatcgc attgaatgaa ttatacaatc gttaccagaa
    28081 accattattc attgttgaaa acggtcttgg agccattgat catatggaag aggatggcac
    28141 gattattgat gattatcgga tcgattattt acgtcgtcat atcgaagcga tggctaatgc
    28201 agtagcagac ggtgtagatt tgatgggcta tacgccttgg ggatgtatcg atcttgtcag
    28261 tgcatcaaca ggagaaatga gtaaacgtta cggctttatc tatgtggatc tagacgatga
    28321 aggaaatggg acactgaatc gatcaaagaa aaaatctttt gattggtata aagaagtaat
    28381 tgcctcaaat ggcgtaaact tgtaattaaa gcagctgaat gaaaaagaac acacttctca
    28441 catgtgattt gtgttctttt tttatttttt atgtgctatt cttttagtaa tagtaaagca
    28501 aagggtgacg ttatgaaaag gacacgtgta ctatacttgg aagtcgcaga taaaattaaa
    28561 gaagatattt tctcaggaaa atatccagtc ggttcgatgt tgccgacaga aagtgaatta
    28621 gaagaactgt ttcaagtcag caaaatcact gtccgaaagg caatcgaact gctggcatca
    28681 gatgaatatg tcgcaaagaa aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac
    28741 aatcgattat ccaaagcggc aagctttacg caaatcttgg agaactcccg atatgaagtc
    28801 cggaaagaaa cgctggattt gcaattggtc catataaaga aggaagatcc tttgttttcc
    28861 tttttcggtg agcaagctgt ttgttttaag cgcttatatt atttgaacaa tcagccttat
    28921 atttattttg ttcattatct tccacaagaa atgatgcaat tcagcagaga agaatttggc
    28981 aaagaatctt tgtatcgttt gctgacgaaa aaaggatata ctattgactc attttctgat
    29041 gatttctcgg cagtattttt aacagaagaa gaacaaggaa ttctgaaaac agacgcacag
    29101 atcggcatca aacggatacg gaaatcgtct acgcaaattg gggaaatgat tgaatattcg
    29161 gaggcggtct accatacgga gctgcatcct taccatattg attatgaaac ataaataaaa
    29221 aaagaggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    29281 ttctcatatt tttcgtttgg ttaggcttat taccgcagat acacggctgt ttctggatta
    29341 ccatttattc tggataaggg ggtgtggcac gattttcaga aggaatctat gaaccaatca
    29401 ctgtattgat tttagcttcg atggtaaccc cattcggctt gacgatcgct tatttcttag
    29461 ggaaaatcat tcgtaaaaat atactcaatc gtcaagaaat cgatacattg aagaccgctt
    29521 ttcctatggg gatctgtcag atcacagagg gatgtttccc tatcgtattg aacgacttgg
    29581 cacgcaatgt catagcaacg ggagtcggtg ggacagtagg tggcggatta agcatgtttt
    29641 ggggcgcaga tagccgtatt ccggcatcgg gaatgtttgc agtcgctacg atgaccagac
    29701 catgggcatt cattggtgca ttgttagctg gttcttttgt cacaggaata accattttat
    29761 tattgaaaaa accagtagat ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag
    29821 acatatcttg ggatgatttg acaattagtt aggagaggca gaatggatta caagaaaaaa
    29881 gtacaataga tgtaccttga gacaggaatg gtatgaaaga atcagagtta gaaagaatcg
    29941 aatatacaga ttttggttta ggagcgatcg aaactgtcca gagcatcggc accctccttg
    30001 aaatgacgat gaaggaaaaa tgagaacctt tcgttgtcga aagggtctgg tataccttat
    30061 gtcgaaggaa aggaaacaaa aaaactttcc tgtactattc caaaaaggaa agaaagttat
    30121 tatacagtaa aaaaagaaat tattcttcat gctagagatc agtacacgtt cgagcccaat
    30181 atcaaacact agttccaggc aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc
    30241 agtgacgatt caagtgatat ttttacaaat ccagctattt atcgttaaca aaaagatttt
    30301 taggagaagc tgatgaacaa aaatgatcaa attttgatcg tttagttcgt cggctttttt
    30361 atttagagaa acatcattta tcagtgaaac ataacacgag aacaaaatga ttttttcgtt
    30421 aataaaagaa acaagtattt gttgtttaaa ataatacacg ttattggcat ataggacgaa
    30481 atcccagtat cttctataag taagtcattt catatcgatg aaaacacgtt atttattttt
    30541 gttttatcta taaaatggct gaaaaatgat ttttttctaa atactatata tagtggcgtc
    30601 tatcctatat accactaaaa aactacaaaa tctagtcttt tattttaaaa aaacacgtgt
    30661 agaatgaaga tagacttttg agttagggag tgttttttta tgaaacttac tgttttttgc
    30721 ggttcccgtt ttggtaataa agaaagttac aaatttattg cgcaaacgtt aggaaaatac
    30781 atggcacaag aagaaatcga actggtttac ggtggttcat actccgggat tatgggagtg
    30841 attagtaaaa cagtactgga aaataacgga aaagtcacag gtatctatcc gaatgggctt
    30901 tttgaaactg aattgcctgg aaaagatgtt actacattta ttcctacaga aacgatcgat
    30961 gaaagaaagg tattattatt tgaaaaggga gatgctgtcc tcgttttccc tggaggtcta
    31021 ggtacattgg aggaattctc acaacttctt tcttggatag caatcggtct gacgcccgac
    31081 aagccaatcg gaatattgaa tattggcggt tattacagcg gcttgcaaca attattagaa
    31141 acttttgcaa aagaggattt tatggataaa aaatggttgg atcaagtaat cttttccaac
    31201 aatccattgg aactggtcaa tgaattacga gcagtatcta ttaaaaatca gttattattg
    31261 aaggaggcaa aataatgagt caaatatatt tagcaggacc atttttttca gaggagcaaa
    31321 tcgatcgagt aagtcgtatc gaaaaagcgt tggaggaaaa caaaacagtc acaagtttct
    31381 actctccacg tcatcaccaa gaaagcaatt atgaactatt cagcgctgga tgggcgcagg
    31441 aagtatacga aaaagatatg gaagaactga caaatgcaga atttgttgtt gctatccttg
    31501 actttgagca tcaaacaatc gatccaggaa cggcttacga attaggtgtg gcaactatgt
    31561 tgaaaaaacc gatgatcatt gtacaagaag agacggtacc tacgaacttg atgattacac
    31621 aaagtctcca tacgtattta aaatcagacc aagctgtacg tgaatatgat tttgaaacat
    31681 tgcctgttga aacttacgta ggcgagtact tatagaagag gaagggttgt atgtcagtga
    31741 ttttgatttc aggaacgatt ggtgcaggta agagcagttt gacggatatg cttgcaaaag
    31801 agattgattc taaaccattt tatgaaaatg tcgaagataa cgaagtattg ccgctctttt
    31861 attcaaatcc tgagcagtac gcttttttat tgcaaatctt ctttttgaac aagcgatttt
    31921 tagcaatgaa aaatgcttta gtgaacgatg acaatgtgct agaccgatcc atctatgaag
    31981 atagcctgtt gttccatcta aacgctgatt taggtcgggt aacggatatc gaagtacagc
    32041 agtacgatag cttattagat acgatgctaa acgagttaga tgatgttgct ccaaaaaaac
    32101 ggcctgattt gatggtccat atcaaagttt ctttggacac gatgctagaa cgtatcaaaa
    32161 aacgtgggcg tgactatgaa cagctagaaa gcgatgagac gttatatacc tattatgaaa
    32221 cgttgaacac ccgctataat caatggtatg aagattttga tgtatgtcct aaaatacaag
    32281 tggatgggga taaattcgat tttgtagaaa atccagaaga tgcaaaaaag gtgatccgtc
    32341 agatccaaga aaaattagct gaattggaag gaaagcctgc tggaaaatat tttacggata
    32401 accagcatgc aaaaggtcaa gaattaggct aaaaataaaa aaccaaacga cgggaaagaa
    32461 aaattactac gtgatgtaga ataagccttt ctatcgtttg gttattttat attcaaatat
    32521 ttgaaataaa taaatcttta ctctttgatt tgtaaactga aatgatccac tgtttgtttt
    32581 actgatgaag cttgatcaaa ggaagaagct gagtttgaag ttcataaaaa tcagccatag
    32641 tgagttttgc ttggatttgt ttatcttctt ctacttttta cataattttg aacaggataa
    32701 gattcaaatg ttttaaatga atctttgact agtttgtgac agttgtcgca tatgctcaag
    32761 cttccttggt cattaaattc tgtatggata tttttgttgt tattacatgt acaataatca
    32821 ggactatcga tcatccattt cgtctccttt acaatcatct tgcttcttta ctatagcaga
    32881 aaagaagggg agaaacaaaa gaagatccaa cagacggcac tatagactgt agatgttgcc
    32941 atcctcatcc ttcaaacggt aattaccgat ttttgtaaat atgactgttg tttctttttt
    33001 attttctttt cctgaaataa tatattcgtc atctttggta gtgatcttga tatctgtcag
    33061 cactgttttt tcttcagatg attcagaaga atcagttcct tgtgtctgat acgtgcgtgt
    33121 ccattccatt tttttatctg taaatgtcaa ctgtcccgcg gataaatcca tatctttacg
    33181 tacttttagc gtatatacac tgttggtggt atctgctttt tccgctttta agacagaaga
    33241 atcggttgtt tcgtccgagg attggtcaga gccgccaaac aaatcacagc cagtcaatgt
    33301 caaaagtccc gcagacaaaa taaatagtac gattaatttc ttcaaaatac ttcccccatt
    33361 tgataggata aagaaattat accattggaa tgtaaaactt caaacggatg gaagagaaaa
    33421 acgcacaaaa cacccacaaa tagagtaaat tttagaataa aattttaaaa aggcaaaaaa
    33481 tccgccactt tcagaaagcg ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat
    33541 taaaaatgaa ccggttcaaa tacttgttta agtataatta atagtgagag gaagttgctc
    33601 tactgaagac tttagttata tggatattga tgacaagcaa cactatgcaa agaatgcggt
    33661 catcataatc gaacaacaag aaagagaaat caaatgtaac tggcaggagt gagaatgtat
    33721 ggcatcgatc cgtgatgtgg ctaagatggc tggtgtttca gtgggaacag tttcccgtta
    33781 cttgaatggt cagcaactga aggaaaagaa catgaaaaaa atagcagaag cgatttcagc
    33841 gttggactat aaagaaaaca tcattgctaa agggttgaag aataatcgaa gcttctctat
    33901 tggacttttg ataaacggga tttccagccg ttttgggtcg gaagttgtct cagggatcga
    33961 aagagtagct gaagaaaatg ggtatagctt attattaagc gggtttgccg atcagccaga
    34021 aaaaattgaa caaaaaattg aatacctgat gaagcatgtg atcgatgggc tgattgtctt
    34081 cttatccgaa gaagagtggt ctggatttga aatgctcaca aaaatgagta ttccagttat
    34141 cgcattgaat tgtccagaag gaccagcagg tattgataca atattagtga atgatcgaga
    34201 aagtgtagca aaagtgatcg ctcatcatgg tgaacaagga cataaaaaaa tcggatttat
    34261 tgctgccact caaacagact acgtagcaag agaacgttta gcaggagcca aagaagcggt
    34321 ccaaaatgat ccatcgatcc aattagaggt ctttacagga gactattcta gaaaaagcgg
    34381 ctattacggg gcaaaagcat tattagaaaa aggggcaact gctatttttg tcagcaatta
    34441 caatatgtcg attggtgcca tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga
    34501 tattgcgttc agtcactatg attatttaga taaaaacgat ctgagtatcc ttccgaaaat
    34561 cacgatcacg ccaccaacag ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg
    34621 aatcaaaaaa gaagaggaag aaaatgaaaa agtgattgtg atggaaaata tcatacatgg
    34681 aatcaactaa aacgaactaa aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc
    34741 cagcaaattt ttggtggggt tctgcagcaa gtggcccaca aacagaagga aggatttcag
    34801 gcgatggaaa aggagagaac atttttgatt attggtacaa aaaagaaccg caaaagttct
    34861 ttgatcaagt aggacctgaa aaaacctcgc aagtttatac gaagtatcaa gaggatgtac
    34921 agttgatgaa acagactggg cataattcat ttcgaacatc tatccaatgg agccgtttga
    34981 tacctgatga atctggaaca atcaacgaag aagccgttcg tttctatcat cgttattttg
    35041 atgagttgat tgaaaacggt atcgaaccct ttgtgaatct gtatcatttc gatatgccga
    35101 tgtatctgca agaaaaaggc ggatggttga accgagaaac ggttgaagcc tatgaaaaat
    35161 atgccaaaag ttgttttgaa ttgtttggtt cgaaagtaaa aaaatggttt actcataatg
    35221 aaccaatcgt tccagtcgaa gcaggttatt tatatcgatg gcattatcca gaagagtcag
    35281 atatgaaaaa agcgatccaa gttggctatc atgaagcgtt ggcttcagcg ttagccatca
    35341 aatcttacca tgaaatggag caaggtggtg ttattggtat catcttgaat ctgacaccaa
    35401 gctatccaag agacgaatca aaccctgaag atgttaaagc agcgcaaata gcagatgcgt
    35461 tcttcaatcg ttcattcctt gatccagctg taaaaggcga gtttccaagt gaattgattg
    35521 agatagtaaa agaacttgat ctcatgcctg tgatccatgc agaggatttg acggttatca
    35581 aagaaaatac agtagatctt ttaggtatca attattacca gccacgccgg gtaaaagcaa
    35641 aagaaacacc aatcgatact aaaaacggtc caatgcctga ggattatttc gacaattatg
    35701 aaatgcctgg tcgtaaaatg aatccttatc gcggatggga gatctatgaa caaggaatct
    35761 atgatatttt gatcaatgta cgagatcatt atggaaatat cgactgcttt atttctgaaa
    35821 acggtatggg tgtagaaaac gaagcacgat ttatcaaaga agatggcatg atccacgatg
    35881 attatcggat cgaatttgtc caatctcatc tgaaatatgt ccatcaagcc attcaagaag
    35941 gtgccaattg tttaggctat catatgtgga catgtatgga taactggtcg tggaccaatg
    36001 catataagaa tcgatatggc tttatttcag tagacttagc acaagatgga aaaagaacaa
    36061 tcaaaaaatc gggtcattgg ttcaaagacg tatcagataa aaacggattt gaggcttaag
    36121 aaagaatagt tgagagcaaa tgcgtgaaat tctccattct taccattttg tgaaaaagaa
    36181 gttcaattat ttacgatctt tttcaacttt tttgtctttc tatttcattt tttgtatata
    36241 atagaatagt caaaattaaa taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct
    36301 tctcttttgg ttattcggtt ttttcttact gcttatttta tccgcagcat tttcaggaaa
    36361 aatgacagca gatcttgctc agaaagaaac agaaaaaatc gtagcaagca aagaatcagt
    36421 agataatttc aaaaaagaat tctcaactta ttggtgggcg gagtataaaa taactgccaa
    36481 ttacgaaaaa ggcgaattgg acatttattt gccaaaagga aaagaagaaa atcaatttat
    36541 taatatcgat gacgtactta gtgcgatttc tctcagacaa tatcaattta aagacaagca
    36601 tttgaccgtt ttgcttttga ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa
    36661 agtgatcacc gcttattctg aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga
    36721 atggaaaaag acaaatgaac aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc
    36781 gacaggaaca agtccttcag attctggcag tggacaaacg ataacgggaa gtgaagaaaa
    36841 tcaagcctca attattacga atgagtcatc acaaggacaa tatcaatcct atggacaatg
    36901 aaaaacagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca aacggcaaaa
    36961 tagttcctca tattttgccg ttttttgagc taataccgaa ggtcagctga ttgaacaccg
    37021 tgtatcaaga ggtcttgagc ctgccgttta actctgagcc acaaggagca atttcaaaaa
    37081 atagctcctt atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
    37141 ccgtttacca agaggttgta aaattagcgt cctgacataa tctaataccg agggtcagct
    37201 gattgaacac cgtgtatcaa gaggtcttga gcctgccgtt taactctgag ccacaaggaa
    37261 caatttcaaa aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg
    37321 gcaggagaac accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag
    37381 gagctgcgac aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct
    37441 tttgaatacc gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc
    37501 gcttacatcg cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata
    37561 aaaaaatctt aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga
    37621 aaaaatgtga atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc
    37681 gtttagcaat cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact
    37741 atgcagattt ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc
    37801 caattgcgac taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag
    37861 cgttagctat tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta
    37921 ttttttatcg ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag
    37981 tgtcgcaagg attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact
    38041 ggctggatat cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta
    38101 tcaaaagcaa ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta
    38161 ttttttcagg aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat
    38221 ttgaccgttc atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa
    38281 tcaaaacagg aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag
    38341 ttttagatta tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta
    38401 aagggaaaaa tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga
    38461 aggtagacgg acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga
    38521 tcagctttga caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata
    38581 tgttggaaac tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag
    38641 acaatgtctt tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg
    38701 ttttccatgg gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt
    38761 atgacaattt ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga
    38821 tattagataa agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt
    38881 tttacacgaa atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt
    38941 tcgactttcc atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac
    39001 attatctgga tcaatcattg gaacaatttt ttacgtattt gaaaaaatct ggtatttacc
    39061 aaaattcaat ctttgtgatt tacggggatc attttgggat ctcgaataca gataataaag
    39121 atttagcaag cgctcttggt aaagatccag atacatggga tgaatttgac aacgcacaga
    39181 tgcaacgagt gccattgatg atccatatgc ctggttatac taaagggaca gtcaatcatg
    39241 aatacggtgg agaaatcgat gttttgccga cattactaca tctattggga atcgatgata
    39301 aagattatct tcattttggt acagacttat tatcttctca gcatgatcaa gtagttgctt
    39361 ttcgaaatgg gaactttgtg acacccaaat acaccgtcct aggaggaaaa gcttacaata
    39421 atcagacagg agaaatcatc gatacgaaag cagcaggaat cgatgaggaa atctccaaag
    39481 atcaggaaaa agtcaagact gcgttaagtc tttcagataa gctgaatcag gaaaatctct
    39541 tgcggtttta tgtgccaaac ggatttgaga cgattgatcc gaaaaaatat gactataaaa
    39601 atgaagcgga acgtaatgaa gcaatcgaaa agcaaaaagg cgaacattcg acaagtgtct
    39661 tttcaaaaaa tggtaataaa acaacaacca acttatatcc agtaacaaga acagacgttg
    39721 aacagcaaaa aaataatcaa taaacaatca aaccaacctt cagtagttag acaacattct
    39781 aatgcttgaa ggttggtatt ttttatgaaa aaaacatttt tttatttttt tcgtgtattt
    39841 ttccttttta acgtatctat ttattgaatg tggaaaaatt taaattaaaa aggagaacta
    39901 ttatgtatga tggtgaattg atcaagaaac ttcgagtcaa caagaagttg acacagtccc
    39961 aattagcgga aggaatctgt tttaagactt ctctagtcgg gattgagaca aattcagtga
    40021 aaaaaatgtc atttgtaaca ttgagatcat ttttagagcg aatgaacatg acacttgcag
    40081 aatatgaatg gatgcgaaac cagctggatg aaccaataaa gttaaagaaa aaaagacgaa
    40141 tgctagatcg ggtacaagaa gacagctttg atccttataa agagatcagc aataaccgta
    40201 ggcattttaa gaaaacagaa gatctgtatt atttggtttt gaatcttcac atggtcgaaa
    40261 aaacagaagt atctgagaca gaatttgaat ttttacgtta tgaatgccga atcattgaag
    40321 aatacttgat gcagatacca gactatggtc aatttgaact atctattcta gctgattttc
    40381 cttggatatt cagtgatcgg ttcattgaga acaattattt gaaaatcaaa aaaagaatgc
    40441 gacgattagc acagttgaac aatcatgagc agtatctctt tactttttta atgaacctga
    40501 cttcgtttta tatagaaaat ggtcggttga aaaaggcacg tagtgtgaat gaagatatga
    40561 aacggagctt agcacataaa gaaagaagta cagtgatcta tgaaacattg atggcagatt
    40621 attatcaaag attgatttct gctgctttag gggaagaaaa gacgaaagac taccaagaat
    40681 tcttcacagt gctcgaatat atgttaggaa agaatcaacg gaggtcctta gagaaaaaac
    40741 tgctggccat tgctgaaccc agcaactaga ccagcagttt agaatttgta tcacttcgcc
    40801 gctttttcag acggtttgtt tatatcgaat ggcgggaaaa agcgggcgat ctcttttacc
    40861 gcagcatcac gggaatcaga agcgtgaatg atgttttcag tccccggcag cgcgtagtct
    40921 cctcggatcg tacctggaac agcatcggca gcttttgttg ctccaaccat cttacggacg
    40981 atatcgatca cttcctcgcc gaccagcaca agataaacga caggaccgga agtcatgtaa
    41041 tcgatcagtt catcaaaaaa actgcgctca gtcaagtgct gataatgttc ttttgctaat
    41101 tcaggagtca tagtttcaaa cttcatttca gcaatggcca gtccctttgc ttcaaaacgt
    41161 tggatgatgc ttccgactaa atggcgtctc acgccgtctg gcttgataat aacaagtgtt
    41221 ctttccataa cagcacctcg tttcctacta tatttctatt atagcgggag gtggaaaatg
    41281 agacaaataa aaccgcgtga gacaggaata taacgtgtgt attttaaaaa aagtatgaaa
    41341 aacatggaaa attgatttga catcctcttt tttagttgtt atattaacga aggaaaaccg
    41401 aataagattt gttaggtgag gctcctatac aaacataggc tactgcccaa aaacgtcgag
    41461 agacgccaat gggtagaaca ggaattgtcg agataaggct tttcataagg tagctaaaag
    41521 caggtatctg ctttttacgt tgtacagtgt caaaactcaa cgatgagatc tgagaagctt
    41581 tttttaatat gctttttcaa acctttcgct gagtatttgg cgagaggttt ttttgttgcc
    41641 gcaaatcccc atcggtgaaa aataaaaagg aggtggtcta attggaaaaa taactattcg
    41701 aaaaatgatt ggaagtcatg aattaaaatt acttagtaaa ggagatgaga acaaatggat
    41761 gacagtcatt cgaaaaacaa ggatctagag gaaaggccag ggctattatc tatctgttct
    41821 cagccccttt cagttttttc tctatcgatc cgatgaatag ctaaagacca aaagcagcga
    41881 gaacagctgt cttttggaca caaaggagag attgagatgg aaaaaaagaa agatacacaa
    41941 aaaatctatg cagcttttgc tggaaaaacg aaacaagaat tatttgaact attccatgca
    42001 tcagcaaatg gattgacaga agaacaaatc gaacgttcaa gagaaaatta cggtgaaaat
    42061 gcgatttcat atggcaaaaa aacaccattc attgtagaaa tattgaaagc atacatcacg
    42121 ccatttacat tagtgttgat cgcattagga attatttcat tcatcacgga atatgtatta
    42181 gcagctcctg gagataaaga tcttttcggt gtagccatta tcttcactat ggttatcgtc
    42241 agcgggacaa tgacgttgat tcagtccgta cgttccaatc aagcggctga aaaactgaaa
    42301 tcacttgtga aagtaacggc agctgtaaaa cgtaatggaa agtacacaga gattcctatg
    42361 gaagaaatcg tctgtggaga tcttgttcgg ctttcagcag gggatatgat accagcggat
    42421 atgcgtttat tgtccgcaaa ggatttattc atttcccaag cagcattgac aggggaaagc
    42481 tatccagtag aaaagcatgc gaaagtatgt gaagacgatc agcaaagcga aacaagttac
    42541 gaaaatcttg tctttatggg aagtaatgtc atcagtggaa gtgcggaagg aatcattgtt
    42601 tctgttggaa atgaaacact atttggtcat gtagcagaaa cgctatctga aaaaccaatt
    42661 caatcaagct ttgaattagg catccataaa acatccatgc tactgatcaa atttatgtca
    42721 ttgatggctc cagcagttat cgtcatcaat gggcttacaa aaggggattg gctagaagct
    42781 tttctttttg gactatctgt agcagttggt ctaacacctg aaatgcttcc aatgatcgtt
    42841 acgacgaatt tggtcaaagg agcaagtgtg atggccaaaa aaggaacagt gatcaaaaat
    42901 ttgaatgcca tccagaactt tggtgcaatc gatgtgttat gtacggataa aacggggaca
    42961 ttgacacaag ataaaatcat tcttgaatat catatggatt gtgaaggaaa tgaagatgat
    43021 cgtgttttgc gccatgcgta tctaaatagc tactatcaaa cagggctgaa aaatttgtta
    43081 gatgtggcta tcattgatga agcaacagaa actttagata cagataagat caattatcaa
    43141 aaagtggatg aaattccttt tgattttgaa cgtcgtcgta tgagtgttgt ggttgctgat
    43201 ccagctggta aaacacaaat gatcacaaaa ggcgcgattg aagaaatgct gaacatttct
    43261 aaatttattg atcttggcgg tactgttaca ccattaacaa aagaacgaaa agaagccgta
    43321 cttgcaaaag tacagtcact taatgaagat ggcttacgag tgattggtgt cgcacaaaag
    43381 accaatccaa gcgtcgttgg agaattttca gtcaaagatg agtcagacat ggttttgatt
    43441 ggttatcttg ctttccttga tccaccaaaa gaaacaacga aacaagcgtt gaaagctctg
    43501 aaagaacatg gtgtagctgt aaaagttctg actggggaca acgaattagt tacacgttca
    43561 gtgtgccgtc aagtaggtct agagatcaat gaattggtta ccggtgagaa aatctcggaa
    43621 atgagtgatc atgaattagc acaagtagct gaagaccatg aagtattcgt aaaattgaat
    43681 cctcagcaaa aagcacgttt gacgacagct ttacgtaaaa atggtcatac tgtcggattt
    43741 ttaggagatg gtatcaatga cgctcctgca atgaaagtgg ctgatatcgg tatttctgta
    43801 gatacagctg tggatattgc gaaagaatct gcagacgtga tccttttaga aaaagatttg
    43861 acgattttgg aaagaggcct tctttctgga cgagaaactt ttgggaatat catgaaatat
    43921 atcaaggcta cagcaagttc taattttggc aatatgtttt ccgtgctgat tgcaagtacg
    43981 ttcctgccat ttttaccaat gctgccttta caacttttgt ttttgaatct gatctatgat
    44041 gtttcctgca tttccttgcc atgggatcgg atggataaag aatatttgga cgagccaaaa
    44101 aaatggaatg catctagtat tggggaattc atggtttact ttggtccaac aagttcgata
    44161 tttgatatta ctacttactt gttgatgtat tttgttattt gtcctgctgt agtgggcgga
    44221 agtttccata cattggatag ttcacagcga gtgattttca tcgcattatt ccatgcagga
    44281 tggtttgtcg aatcactatg gacacaaacc ttagtgctcc atgcactgcg tacaccgaaa
    44341 gtaccattta tccaaagcaa cgcaacattt gccatgtttg ttatcactac attaggaatt
    44401 attgtcggtt ctgtccttcc ttttacaagt tttggtgctg aattaggctt gatgccactt
    44461 cctggcaatt actggttatg gttatttgct acagtcatcg cttatctggc gcttgtgaca
    44521 gctgtgaaga aaatctatat ccgtcgattc caagaactct tataaacgag aaggctggtt
    44581 ctttgcttct gttctttttg gaccagtcgt gctaaactat gtgtattgtt ttggaaagga
    44641 ttaatgtata gatgaaaggt gcaatatttg atttagatgg tttgttagtt gacacagaaa
    44701 aaacgtatcg tgatggctgg ctttggggat ttcagcaatt tgatatccat atcccaaaag
    44761 aaatcgttga cgcttggggc ggaaaaaact ggaaacaaag ttttgatatt ttagtgaaag
    44821 cagcaggtag ccctgaaaaa gtacaggaag tccgagcgaa gcgtgaagaa tatttttatc
    44881 aacagctgac aagcggagcc atccacttaa aaccatatgc aaaagaaata ttggcagact
    44941 taaaagaacg tcatatgaaa ctaggcattg ctacgaccac tgttacgaaa agagcaacag
    45001 atatcttgga tcactttaaa ttagctgatt acttggacac gtttacattc ggagacgaag
    45061 tgtcggagaa caagccttca ccgattcctt atctgacagc tttggaacga acaggattag
    45121 aagctagttc agcctttgca gtggaagatt ctttagtagg agcaacagct gcttcacgag
    45181 caggattagg cgttgttttg atacctgatt ccagttttgt ccgaaattat accgtcgaag
    45241 aaaaggaaaa attgaaactt cttgcggaag gaaaagatct tcgaactgtt attgaaatgt
    45301 taaataaaaa aacagaaaaa taaatataga aaaaataatt tgaatttcgt acttgacttt
    45361 tcagttttcc tcatgtagat taatcccaac aacaaaatat cgatttcgtt gaaaaggaaa
    45421 gtaagtttgg aaactttgca gagaggctct ggctggtgag aagagtcaag taagaaaagc
    45481 tgaaaatggc cttggagaat aatttgtcga taagtaggca aattcggaac tgttcgaccg
    45541 ttaaaacgaa cgccaatctc tctttttgag atgttgggct tgagggaagg aaacttccga
    45601 aaaaaggtgg taccgcgata cgtcgtcctt ttactgacat agtttatgtt agtaaaagga
    45661 cttttttttt gaatattatt tgcattgaaa tggtaagtac gatagaagaa cctttgacag
    45721 agagcttcgg tagctgagaa gaagcaagga acgactattg gaaaatggcc atcgagcaat
    45781 tcatcccgag catagatgta aggcgatgac ggctgcgacc gatatcttgc tagagtatga
    45841 tcgtacttga agaggagttg tgtgtgaaca cagcttgaac atgaggtggt aacacgcaaa
    45901 aaagcgtcct caagaaaatg acattttcat tttcttgagg acgctttttt tagataagga
    45961 ggaattttaa tgattgaatt gcgagatgtt tctgtatgtt tcaaacaaaa agaaaaagag
    46021 atacaggcag tagattctgt agatttaacg attgaaaaag gcgatgttta tggaatcgtc
    46081 ggttactcag gtgcagggaa aagtacgtta gttcgtgtga tgaatttgct gcagaaacca
    46141 acagcaggag aagtcattgt caatcagacg aatctgagtc agctctctcc caaagaattg
    46201 cgaaaagaaa gaaaggccat cggtatgatc tttcagcatt tcaatctaat ggaaagccga
    46261 actatttttg acaacgttga tttttcttta aagtattctg gaaaatcgaa acaggaacgt
    46321 cgtcaaaaag tgaatgaact actggaactt gtcggtttgg aagaaaaagc atctgcttat
    46381 cctaaacaac tgtcgggggg acaaaaacaa cgagtagcaa tcgcacgagc tttggcgaat
    46441 gaacctaagg tattattatg tgatgaagca accagtgcac ttgatccgaa aacgaccctt
    46501 cagatccttg cattgttaaa aaaattgaat cgccagttag gtttaacaat cgtcctgatc
    46561 actcatgaga tgcaagttgt caaagaaatc tgcaacaagg tagcggttat ggaagatgga
    46621 agaatcgttg aaaaaggtag cagcatccag atcttcagca atcctgaaga agaattgacc
    46681 aaagatttta ttcgaacagc tactcatctt gaccaagcat tagaaacgat cattgcccat
    46741 tctgcttttg ccgatcagat cgccaataaa tggctggtgg aattaagtta tattggaaat
    46801 cagacgaacg aacctctaat cgctcatctg tacagcaaat atcaagtaac agcgaatatt
    46861 ttatatggca atgtagagtt attacaagaa acgccgttag ggagtttgat cgttacttta
    46921 gctggagaaa ccaaacagcg gaaaaaagca ttggattatt tgatcaaatc tggtgtaaaa
    46981 ataaatattt tacaaaaaaa cgatttagat agtcgtatca aagtgatcga aggaggaaga
    47041 taaaatgact gatttcttta aaacatattt gcccaatgtt tatttgattc cggatgaatt
    47101 tgtcgaagcg acgaagcaaa cactatatat gtctttttgg acagcattca ttggcgggat
    47161 gattggaatc atcttaggtg tgacgttagt agtgacacgc cctaatggat tactagctaa
    47221 ccgtctgcta tttgaaatac ttgataagct gatcaatatc attcgttcca tccctttcat
    47281 tatcttgctt tctcttttag cattgactac tcgtttctta gttggaacga cgattggtgc
    47341 gaaagcagct ttagtgccat tgatttttgg agttgttccg ttttttgctc gtcaaatcga
    47401 gaacgcactg ctagaagttg atcctggggt gatcgaagcg gcagaagcga tgggaaccag
    47461 tccaatcggt atcattttac gtgtgtattt gttagaaggt ttgcctggta tcattcgggt
    47521 ctcggcgttg actatcatca acgtgatcgg actgactacc atggctggag cagtaggtgc
    47581 aggcggatta gggaatcttg ctatatcgag agggtataat cgattccaaa cagatgtaac
    47641 gattgtggct acattattga ttctaatctt agttttttta agtcaattta tcagtaatca
    47701 actgattaaa aaaattacac attgaaagtg gaggagaaaa aatgaaaaaa attattcgtt
    47761 ttgtagtcat tacatttgta gcaattattt ggttggcagg gtgtagccaa ggaaaagcag
    47821 atacaactga caaaaaagaa actatcgtaa aactagggat tattggagaa gatacagatg
    47881 tatgggacaa cgtgaaagac cgtttgaaaa aggagaacat taatttggaa tacgtgaaat
    47941 ttacagatta caatcaacca aatgtggctt tagcagatgg atcgattgat ttgaattctt
    48001 tccaacatca attcttttta gacaattaca ataaagaaca cggaactgat ttagtatcga
    48061 tcggaaatac tgtcaatgca ccgctgggaa tttattctaa cactttgaag gatgtagctg
    48121 atatcaaaga cggagcaaaa gtagcaatcc ctaatgatgt tacaaatggc ggcagagctc
    48181 ttctgttatt gcaaactgct ggattgatca aagtggataa ggcaaaagga caagcgccca
    48241 ctgtttctga tattaccgag aatagaaaaa atctagacat ttctgaatta gatgcttccc
    48301 aaacagcacg tgctttgtca gatgtagaca tttcagtgat caatagcggt gtagctgtag
    48361 atgctggatt taatcctgct tctgatgcca tcttcttaga accagtggat gaccatgcac
    48421 gtccttatgt gaatatcatc gttgcaagaa aagaagatga aaacaataaa acgtatcaaa
    48481 aaatagtaga cgcttatcaa acagaagaca cagtcaaagt aatcgagaaa acatcaaaag
    48541 gatcaagcgt gcctgcatgg gaaacattcg ggagaaaata agaaaaatga ctagcatagg
    48601 aggaattttt tatgacaaca acaatctcaa caaaccaatt gaaggatcta atccaatatc
    48661 gtttaccaga atatcaagca cttgctctag atatccacga acatcctgaa gtaagtaact
    48721 atgagtttta ttcctcagca gcgttgatta aacaattaga aaaagaaagg ttttgggtag
    48781 aaaaagaagt agctggacac cgaacagcct ttgatgcacg ttatatttcc aataaacccg
    48841 gtcccacaat tgcctttttg gcagaattcg atgctttacc aggtattggt catgcatgcg
    48901 ggcataattt gtttggtact tactccgtat tagccgccag catagtgaag caattcattg
    48961 atgaaactgg tggagaaatc cgtgtatatg gcaccccagg ggaagaaggg ggcgagaacg
    49021 gatcagctaa aggcagcttt gttcgagaag gtttttttga ggatgtcgat gtagcgttat
    49081 gcgtccatcc agcttttcgt tatgggaaaa cgacagaatc tttagcgaat gatccagtcg
    49141 acatcaaatt ttacggtgtc gcttcccatg cagcagcagc acctgaaaaa ggagtaaatg
    49201 ctttggaagc attgatccaa gtgtttaatg gaatcaacgc tctacgcttg caattgccaa
    49261 aagatgtcaa tatccatggg atcattacag acggaggagt agctgccaac gtcattccag
    49321 attacgctgc tggacgtttc tatttacgtg caggaaatcg gcaaacatta gataaagtat
    49381 atgaaaaagt ggaaaatatc gtacgaggtg ctgcgataag tacgggcacg acttttgagt
    49441 ttggattgtt ccaaaatgca gttgatgatg tcatcgttac tccttctttt gatgacttat
    49501 tcttccagca tgcaaaagaa gcaggtgttc cagatgaaga aatagaaacg gaacaaagaa
    49561 caagtctcgg ttcttctgat gtaggaaatg tcagccaagt cataccaaca atccagccta
    49621 cggtagcaat ctctgagaca tatattgccg gacacacaga agaattcaaa gctgctgcta
    49681 aaagtgacaa aggccttgct tctattgcga ttgcagcaga actactcgcc aatacagctc
    49741 ttgatctgtt gaaagagcca aagctattag aaagaatcaa gcaagaacat caagaaatag
    49801 tagcaagaaa aaaacaagag ggcgctactt tctagtaaaa aataaacttg tgtgtgaaag
    49861 ttataaaaat aattttcaga aaaatgaaaa aatattttct ttttcattga caaatgatct
    49921 tatggaaggt aaacttcgta caagtcaact tttcacgcag tagcggttag gtgttttgtt
    49981 cagaaagtcg gtggtcgctg tgaaccgatc aagcctaatt gacgaattac atggaaagtc
    50041 catacatttg aataaaagtt tgaaagtgaa aaacagatct gtttttaatt aaggtggtac
    50101 cgcggagtca ttcgtcctta gtttaaaaca ggtcttttta tttttataaa ggaggaatcg
    50161 cggatgaatt gcggcggagt aatccgatat cttaactttt ttagaacata agaacaacta
    50221 aaaaaggaga gattatcatg ggaaaaaaag gaacaccaag ttttttagaa gcaacacttg
    50281 tattaatggt tattgtttta ggcattgcga ttggcgttgt cggattaaag gtctcaccta
    50341 atatcacgat cttagcagcg atcggaatgg tcatgttgta tgcagtgata aaaagatacc
    50401 cgacagattg gctgcacgaa ggaatcatca atggcatcaa accaggaatc atcccaatct
    50461 ttatttttat tttagtcggt tcattgattg ctgtatggat acaagcaggg atcattccaa
    50521 ctctaatggt cattgggttc aaattgatca gtgtccagtg gtttgtccct tctgtatttg
    50581 ttgtgtgtgc aatcgtaggt agcgctgtag ggagtgcctt tactgttatg tcaacgattg
    50641 ggattgcatt tttcggtatt gggacaacac taggcttaaa tccagcgctg gttgttggag
    50701 ctattgtttc aggtgccgtt tttggagata agatgtctcc attatccgaa tcaacaaatc
    50761 tagcagcagc tattgttgat gccgatttgt tcaaacacat caagcatatg atgtggtcaa
    50821 ctgtcccggc atttgttgtt tctttctttt tattcatgat tttaggacac acaagtaaag
    50881 ccgcaagtct ttcatctatt cgtgaagtga ctgatgttct agaagcaaac tttactattt
    50941 caatctggtc actgattcct ttatttttaa tgttactgtg tgcatggaaa aaggtgccgg
    51001 caattttaac gattttgttg aatatcgctg tagcagtagg aatgattttt attcaaaacc
    51061 cacaaacttc cttaacgaag ttggctgggg tcatcgaatc agggtttgtt tcaaatactg
    51121 gaaaccaaca aatcgattca ctgcttagcc gcggaggaat cgaaagtatg atgcctactg
    51181 tgtcattgat tattctaact ttgtcattag gtggattgtt gatcgaattt ggcttgatct
    51241 caaccgtgat ggaacgagta tcaagaaaga tgacgaacac accaaatttg atttttacaa
    51301 cattgatgac aagtatcgga gtaaatattt ttattggtga acaattccta tctgttattc
    51361 tgcctggtaa tgcatttaaa gaaacttatg aaaaagcagg atttgatcca actgtattag
    51421 gtagaacgct agaagatggt ggaacagtaa tcaactactt agtgccttgg ggaattgccg
    51481 gaagtttcgt agcaagtaca tttggtattc ctacactcac ttacttgcca ttcgtatttt
    51541 tcagtttgct ttctcctgtg ttttcgatgg tcagtgcctt cacaggttgg ggaatctcaa
    51601 aaaaatttgt gaaaccgcaa gaagcagtaa gcaagacagc gcaattagaa caataaaaac
    51661 ataaaaaggg attgtgacaa gacttttgtc acaatccctt ttacgaataa acggtattca
    51721 aaagccagtt tctcggtatt aagccaaatt tatacaaaaa tgtggaaagc catttcacaa
    51781 aatttttctt aatacgcgaa actaagcgac tttttcacaa cctctcgata aacggtgttc
    51841 aatcagctga ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tatttttcca
    51901 tttgtctctc taatactcag gtcagaacgc tgattttaca acctcttaat aaacggtgtt
    51961 caaaagcttg catctgcggt aataagccta accaaacgaa aaataggaga agctattttt
    52021 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacccctt attcacggcg
    52081 ttccagaagt agcttcttct tatttcttga agctgataac ttctggaaca tcctcttttt
    52141 ttatttggac ggataatggt cattccattt ctttgatcat ttttttcatt ttgtcgatct
    52201 cttcttcttg tgcctgaatg atttcttccg ctaatttttt tacttcagga tcttttaaat
    52261 cagctcgttt acttgccagg atagcgatag aatgatgagg aatcatggcc ttcatccaag
    52321 ctgtgtcgtt gacagtagtt tggcttcgta ccataaacaa ggaaccggca aaaacaagaa
    52381 gactagcggc taagatcata acatttatct ttttgttttc atacattttc cacatgaaca
    52441 acagcatgat gactgccatg actgcaccca tcatcaacgc catgaatact cgtgtacgac
    52501 tgaagaagat atgatcgagc gcatagacat tcaagtacat cataccaaac ataactaccg
    52561 ttgaaacacc gatcatcaac aaaaacttcc aatacttttt catttaaaac aaactccttt
    52621 ttttcattcc tttcttctat catgtcatga gcatggagaa caatcaatta ttttgcacag
    52681 agggtttttg aacaccgttt accaagaggt catgagactg tttattcgga aaaaggagtt
    52741 gcaacaagac ttttgtcaca actcctttct tcaatagctc catctattcc acataaataa
    52801 aataaaattt tctatcgatt tcattcattc gtcaatacga acgcatcatt ctttttttga
    52861 taactgaaat aaatgaacag gtaaagcagg ccagtcaata cagttacccc aatggagttc
    52921 gtgaacagga taccaaggaa aatcaatcct aagacagcta atgcaaaaaa gctgattcct
    52981 ttatcccata atgttccttt tcttagtgcc agtacaaagg atgccgcact gataatgatc
    53041 cagatcaata atactgtata cgaaagggaa cctgccagat agccaaataa tttgtcaccg
    53101 acaaaataag aaagagctac accaagataa agaacactgg tacaaaataa aacagcacgt
    53161 tgcggtactt gatgcttgtt taaaacagct agttttctag aggaaccttt tcgatccttc
    53221 aaacggaaga acaaaattct tgaagatgca tacacacccg aattgataga tgagaacaaa
    53281 gcaaggacaa tcacaaaatt gacgatatcc gctgcaaatg ggatattcat tttttcaaat
    53341 accatcacaa aaggactgac attcgtgcct gctaaagtat tccaaggaaa aagaatcaaa
    53401 agcaaaaaca aaggaatgat gtaaaaggaa atgattcttc ccatgacacc tttgattgct
    53461 ttaggaatcg cacgtttagg atcatctgct tcgcttaccg tgatagcaat cagttcagaa
    53521 ccgccgtaag aatagataac tacaagcaaa gaactgatga ttcccttcat accatgagga
    53581 gtaaacccac catggtcagt caattgctga agggtaggga aaacgcctgt gcccaacatt
    53641 tgctgaccga caagataagt agcaaaaatg atcagtaaga taatgacact gattttagca
    53701 aaagccagcc aatattctgt ttctgcgaat aaccgtaccg aataaagatt gatgatcgta
    53761 gttaaaacag ccaacataaa aacaaatacc caggctggaa tgtgcgggaa ccacaactgc
    53821 aaaaaggaag cagctgcaac agcttcagcg atgatattga taatccacat agaccagtag
    53881 acccaatcag taaaatcagc agaatgagca cctagataag ggcgtatcaa accagataat
    53941 ccgtgatgcg tgtcgccacc gcttaaaacg atttttccca gacctttcat cacgatatat
    54001 aaaatcagcc caccaatcaa aaaagcaatc aatacagaag ggccggcgat cccaatcgct
    54061 tcgccacttc ctttaaatag acctgcaccg attgctccac ctaaagctaa catggtgata
    54121 tgtctggagc taagttgttt tttcaatttt ttatcttcca taaatgacct ctttctttta
    54181 aattcacaat caaaatttat attaagaata aatagcaata tttaacaatg actaatgata
    54241 acaaattttc agacaattac aactattcag aaaactttta ggcgaatttt aatagaagaa
    54301 tgatttattt catcatattt tcatacaaat gttaaatttt catacaaata taggaaacgt
    54361 tttactggct ttttactatt taaacaggct atcataggaa aaagtgacag agaaagtgag
    54421 gcggaaaaga tgaacaaaaa tgacgtactg ctagttgcaa aaaataaatc actttatttt
    54481 ttgccttcca tccgctcttt agatgtttgg tccggaaaag atttactaca gtcggaattc
    54541 attaaatcag ctagacaaca gttggcaaat tatcagctga tcgttttttt agattatgga
    54601 tttgatatgg agatggctcg acgcgtgcgc ccatttacca atgcaaaaat tgtgttattt
    54661 ttttggaatc acttcaaagc agaacacgag gcgatgctga agcaggcaca agatgaaccg
    54721 gcaatcgatg agatttatca ttttgatttt ttggaagcaa aagaacttgg gctgaaacat
    54781 aatagttcat tttattcgaa acatatgaat ttttcttcta gcgaaccaaa gacggatcta
    54841 ttttttggtg caacgaataa tgggagaaaa gaacgagcag aaagctataa gcaggaattt
    54901 ataaaaagag atgttacgac caactatttc attcttccac atcgagggaa tgaacaacct
    54961 ggatatctta cttatgaaga gtacctgaaa aaaacagggg aatcgcgcgg gattctggaa
    55021 ctgttgcggg agggtcagca aggagtgact ttgcggacat ttgaatccat gtattttcaa
    55081 aagaaactag tgacggataa tcaagcgatt gctcattatc atttttatcg tcctgaaaat
    55141 atttttttgc ttcgagaacg tgatttagat gaattaccgg aatttctgca tacaccattt
    55201 aagccaatcc tatcagagct attggaattt tatgatgcag aaaattgggc aaaacgattt
    55261 atcactaacg attccactat ttatgagcaa tacgaatatg acctctcctc tgttgaaaac
    55321 aaataaaaaa cctacatttt gaagagatta tgtactgaaa caaacaagca caattcttat
    55381 ggcatcatga gagttgtgtt ttctttttga tcttttattg ggcttaaaaa aagagaacca
    55441 gctgctgatt cagctgattc tcatcagaga gccttttatt ctgcgccaag gattccttct
    55501 tttgcatgga ctaattgggt caaaaaggca caatatggtg tagcaatatc atatttttgt
    55561 ccttttcgcc aaatggcacc attgatgtag tcgatttcag tcaaacgatg gtttttgatc
    55621 aggtcttggt acatagatgg gtaatgcaac ccgatgttgt caggatcata agtagaagca
    55681 atatgttggt acacttcttc ttgatctagt atgattcctt cttttgctgc gatagaagca
    55741 aattcagata cgattgttcg aaccatgctc tctgccgctt tttgcgcgcc tagttcagca
    55801 atattacaat ccaaaatcgt gcataaacca ttcaatgttc catttacaca agcttttcgc
    55861 caaatggaat aacgaacgtt atggctatag atcggattta aatttgcttc ttgaaagact
    55921 tctacaactt tttttgtaaa tgcttcgcct tctggttcca agttctctag ttcgatttct
    55981 ccatcaccta acaatgtgac ttttccggga ccagctagac cagctgtcca catcgtgatc
    56041 ccaaataaga tatttttctt tggcacatat ttttccagta catcttcatg acctaatcca
    56101 tttagtaaac acaaaacata agtattgtca gtaataatgg attggatcga acaaaacata
    56161 tcatctaatt gattggcttt tgttaatgct acgatcaagt ccacgtgttc gttgctttcg
    56221 atgatttctt ctggtgaata gattggcagt tttgcgacca cttctttacc gttaaagtca
    56281 gcaatcaacc catttgtacg aatggcttca atgtgagcag gccaacgatc gatcaagagg
    56341 acctcatttc cgctttgatg tagcatcaat ccgattcggc tgcccattgc tccggcccca
    56401 gctattgcga ttttcatgtt ttttctctcc tttattcaat gagttccctt ttagtatata
    56461 gaaaaatgga agaaaatagc attgttttgt ttgaaaaaaa tgaaaaaaag tgtcttactt
    56521 aagaagaaag tgagggatac agatggatat tttggcggca attgattcat ttaaaggaag
    56581 tgcttcttca tttgaactaa atcaggcagt tctaaatggc tgggaagatc gtaaaggaag
    56641 aaaaatcaat gtgccgattg cagatggcgg agaaaatact gcagaagcaa tctattacgc
    56701 gctcagggga acatggcgta ccattgatgg gatggatctc ttattccgtc agcgaaaaat
    56761 cagctattta ttgacaactt ggcaaggaaa aaacaccgca gtcatcgaat ctgcacaagc
    56821 attggggtta gatctacttg ataaaccaac ggatcaaact atccgacttg cttccagtta
    56881 tgccttagga gaacttatta aagatgcact gcttcaagaa gtagaacaaa ttattgtcac
    56941 tttaggagga agcgggtgta gcgatggtgg tctaggatta ctccaatcct tgggtgcttc
    57001 tttagaaggg tacacagaag gaaatccttt attatctact aaaaaacttc attttgatcg
    57061 aagaaacgtt cttgtttcta aagtaaaatt gattatcgct gcagatgtta ccaatccata
    57121 tacaggtcaa caaggagctg cttggcaatt tggaagacaa aaaggaggaa tagatacgac
    57181 gctagctttc ttagataaac aagcaggaaa gattgctcag caaataaaag atttctggga
    57241 ggtggattta gatgatattc caggaagtgg agcagcaggc ggtattggtg gagccctttt
    57301 tttgttggga gcgaagatgg tttctggttt cgatttagtc agccaaatca ctggattgga
    57361 aaaaaagatc gaacaagcag atgtcatcat tacaggagaa gggcggatcg atcagcaaac
    57421 gatccacgga aaagttccat acggtgtagc agtgcttgca aaaaaatatc gaaaaccaat
    57481 aatcgctatt tgcggaagta gagaccaaga gctagaagga ctttctgatt ttctcccaat
    57541 tgtcttaagt attcatttgg gtccaatagg tttagaagaa gcaatggaac acaggcaaac
    57601 attagaaaaa acaaaaatct taggacagac acttagcaga tttttcacca tcctcccgaa
    57661 gtagttgaca aaatcatttt ctttatgcta tcgtaatttc aaatatagga caaagtgagg
    57721 gatccgtgca ttgaaaaatt aagtcgattg atgaaagctg cattcagact gtaccttgtg
    57781 gagacaagga agcagtgtgc agattttcgg gattggcttg gttttttcga tgtatgggtc
    57841 tttttgtgtg tatccaaccc ttgcatcctc tttagccagt tctgaaaata ggactggtct
    57901 ttttttacga ggtgatggaa aatgtctaaa atcgaaataa aaaatctgac attcggctac
    57961 gacagccaag gcacattatt atttgaacaa gcaaatctaa attttgacac acaatggaaa
    58021 ctaggactta tcggacgaaa cggtcgagga aagacaactt tactgaatat tctacaaaac
    58081 aaactacctt atcaagggca agtaatccat cagcaagaat ttgcctattt cccgcaacag
    58141 acaaaagata aagaacgttt aacctattac gtgttaaatg atattacgga ttttgagata
    58201 tgggaaatcg aaagagagct ccaattgatg caaacagatc ctgaaatctt atggagagaa
    58261 ttcagcacac tatcgggggg agagaagaca aaagtcctac tggcactttt atttgtggat
    58321 gacactcatt tcccgttaat cgatgaacca acgaatcatt tggatatctc tggtagaaaa
    58381 caagtagcgg cttatttgaa aaagaaaaaa caaggcttca tcgtggtcag ccatgaccgg
    58441 ggatttatcg atgaagtagt ggaccatgtt ttagcaatcg aaaaaagtca actggaactt
    58501 tatcaaggga atttctctat ctatgaagaa cagaaaaaac ttcgtgatga atttgaaatg
    58561 gctcaaaatg aaaaattgaa aaaagaagtc agtaggctaa agaaaacagc agctgaaaaa
    58621 gccgaatggt ctcgttcccg agaaggagat aaaacaaaga aacaagtcgg attcatcgat
    58681 actgaatcta gacgagtgaa taaaggagca gtgggtgctg atgctgcacg gacgatgaaa
    58741 cgatccaaag caatcgtgaa tcggatggag acccagatca gcgagaaaga aaaactatta
    58801 aaagatatcg aatatatcga ttctttgacg atgaatagcc aagcgtctca ccataagcga
    58861 cttttaagcg tagaagatct tcaattaggg tatgaaaatc tgttattcga gccaattcat
    58921 tttacaatcg agcctcatca gcgggtggcg atttcaggtc ctaacggtgc aggaaagtca
    58981 tccattatcc attatcttct gggggcattc aacggcaagg ttataggaga aaaataccag
    59041 ccaaaacatc tgagcattag ttatgcaagc caaaattatg aagacaatcg aggaacgttg
    59101 gcggaatttg cagagaaaaa ccaagtagac taccaagcat ttttgaacaa cctccgaaag
    59161 cttgggatgg aaagagatgt ttttcataac aagatcgagc agatgagtat gggccaacgg
    59221 aaaaaagtgg aattggctaa atctttatca cagccagctg aactatatac atgggatgaa
    59281 ccattgaatt atttggatgt cttcaatcaa gaacaattag aacaactgat cttgaacgtg
    59341 aaacctgcca tgttactagt ggaacatgat caaaccttcc tggataaagt atctactgag
    59401 attatttctc ttgagagaat ctaacccttg taatctcaag aacaacaagg tatggttaag
    59461 gggaactttg ccaacgaagg agagaatcac atgctttatg taacgagaca cggagaaaca
    59521 acttggaacg cacaaggatt agtttgcgga cgggcagatg tgccgctgac tgaaaaagga
    59581 cagatgcagg cacagaaact agcagaaaag gtagtagatt tgcctgttcc aatcacgaaa
    59641 atcatccatt cccctcttca acgagcaaga gatacagcac aagcagttgc tgatcgtctc
    59701 agcttgccgc tgaccgttga tgaacgattg gtagaaatgg attttgggga ttatgatgga
    59761 ctgccaagca aagatgagaa ctttcaaaaa gcacgtttag cgttcgctgt acgattccca
    59821 aatggggagt cagtcttaga tgtgtatgca cggatcgttc ctttattgaa agaatgcatc
    59881 gaagacgaag aaaatgtcta tctgttagtt tgccataatg ccttgataag agtgatcaat
    59941 gcttattttc atccgatgcc taatgagggg ttctttacat tcatggtaga taatacagaa
    60001 ttggtttctt atgagtaatt atgtttttgt gttatttaaa taaaatattt caaggcactg
    60061 cttttgctta gcagtgcctt ttttaggtta tgacaattct tttgataata taaactcaac
    60121 ctttagaaat gaaatttaat tattttaata aaagtattga aattttgttt ctttagatat
    60181 atactctagt catagaaatg aagacggata cagaaaaaga gggataatat gtttggcttt
    60241 tcaatattta tgaacgattc tttgtcagaa gagaagaaac agtatatccg ccaaatggca
    60301 tccaatggct ttgtcggtat tttcacttcc atgcatatac cagaagatga tgtttcggca
    60361 tatcaaaagc gattgatcga actaggaagc gaagcgaaaa agaataaact agaattgatg
    60421 gtcgatattt caggagaggc attggaacga gcgggttttt cattagaaga tctaacgcct
    60481 ttgaaagcaa ttggtgtaac aggtttaaga atggattatc atatttccaa tcagcagatt
    60541 gccgactggt cacatcaatt gaaaatcagt ttgaatgcta gcacgatcac gcctaaagat
    60601 atcgatgaat tgaaagaagc agaagctgat ttctcacaga ttgaagcttg gcataattat
    60661 tatccacgtc ccgaaacagg gctggacaaa gaatggtatc agaaaaaaaa tcaatggctc
    60721 aaaattcaag gtctccttgt ccaaggtttt gtaccaggag atacagagtt gagaggccca
    60781 ctatatcaag gattgccaac tttggaagaa catcgaggga tccatcctct tgcaggcgct
    60841 ctagatctat tagcaagtaa cacggatatt gtttacatcg gagatgcagg tctgtcagaa
    60901 aacgttcagg agcaatttgc ttctttccaa aaagaacaga cagtcttgct ccatacggaa
    60961 cctgtagatg aagaatttta cgaatatata ttagggaagc atacgaatcg gcaagacgat
    61021 gcaagagatg tcatacgaag cgtagatgca cgttttcgag agattccgcc tatcccagca
    61081 agaaatacag ctacgcgtat gaaagggtct attactttag ataacgaaaa gtatttgcgc
    61141 tatatgggtg agatccaatt aaccaaatat gatttgccag cggatgaaaa agtgaatgta
    61201 gtagctaaag ttatcaaaga agagctacca ttgatcaacc aaatcaaagc tggtatgaac
    61261 tatcaattta taagaaaaga aggaagataa aaatgagcaa aatcaattta gagaacctaa
    61321 caacggaacg cagaaaccaa gaaacttttg gattagatga gatgagtatt tctcaagccg
    61381 ttcaactaat gaacaaagaa gatcagaaag tggccgctgc ggttgaaaaa gaactgacaa
    61441 gtatcgaacc agtgattgct aacacgattg agtcctttaa aaaaggtgga cgtctgattt
    61501 atatgggagc cggaacaagt ggaagactag gtgtgttaga tgctgctgaa tgcgtaccga
    61561 cattcggtgt agaaccggaa atggtcatag gtctaattgc tggtggagaa gaagcaatga
    61621 ctgtagctgt cgaaggggcc gaagattctt atgagctagg gcaagaagat ctaatcaagc
    61681 tgaatttgac aaaaaatgat atagttattg gtatcgcagc tagcggtcgt acaccatatg
    61741 tgatcggtgg attagattac gcaaaatcga tcggtgctgt cacaggaacg atttcttgca
    61801 ataaacaagc cgaaatcagt aaacacgcgg atttcccaat cgaagtcgat gcaggtccag
    61861 aatttttgac aggctctact cgattgaagt caggaacagc acaaaaactt atcttgaata
    61921 tgatttctac tatcagtatg attggtatag gtaaagtatt caataacttg atggtcgatg
    61981 tcaaaccaac aaatgaaaaa ctaatcgaac gctcaaaacg aatcatcatg caagcgacag
    62041 aagctgatta cgaaacagct gaaaagtact ttgaagaagc agatcaaaat gttaagctgg
    62101 ctatcgtcat gattttgaca aatagtgata aagaggaagc tcaagcaaaa ttagctgaag
    62161 caaacggatt tgtaaaaggg accttatgag ggggaaaaga ccatggcaaa aaacgaactg
    62221 actttggaag aactggcaaa acaaatttat aaaggggcag gcggcatggg caatgtagaa
    62281 agcattgtcc actgcatgac tcgtgtgcgt atgaagatca tcaatgatga tcttgtaaat
    62341 gtctctgaat taaaagcgat tccaggagtg cttggtgtag tagaagatga acagcttcaa
    62401 gtcatcatcg gacctgggaa agtgaacaaa gtagcacagc aaatggttga tttggcaggc
    62461 gtgaaattag gagaaaaact ttcttctgta agtacagcta cagcaaattc atcagcatct
    62521 ggaaaagacc gtgtaaacga acgggcacaa gaaatgaaag cggcacaaaa atcgaaacaa
    62581 aaaccatcta aaatcaaatc ggtattaaaa gacatttcta atatctttgt gccaatgatt
    62641 cctgcatttg tcggtactgg gatcgtggca gggattgccg cagttttagc aaacttagtc
    62701 acagccggaa cacttgatgc agcaacatgg caacaatata tcgatgtgat gaacatcttg
    62761 aaaaacggga tgtttagcta cctagttatc tatacaggta tcaatgctgc acaagttttt
    62821 ggtgcaacac caactcttgg tggagttatc ggggccgttg tgatgctgac agggatgaat
    62881 ccggaagcac cactgaaaaa cttatttact ggtgaagctc ttgcagctgg acaaggtggg
    62941 atcctaggtg tcatcttcgc tgtttggcta ttgtcaatcg ttgagaaaaa actgcataaa
    63001 gtagtacctg atgcggtaga tatcatcgtt acaccaactc tttcattgat tgccatcggt
    63061 ctgattgaaa ttttcttcat catgccttta gcaggtttca tttctgatgg catggtcggt
    63121 gggatcaact gggtacttgg cgttggtgga gcatttgccg gcttcgttct aggcacatta
    63181 ttcttgccga tggttatgtt cggactgcat caaattttga ctccaatcca tgtgcaaatg
    63241 attgatgaaa ctggtcgtac gctattgctt ccaattcttg cgatggctgg tgcaggacaa
    63301 gtgggtgcag cattggcatt atggattcgc tgcaaaaaag ataaagaatt aacagaaatg
    63361 atcaaaggag ctttaccagt cgggatccta ggaatcggcg aaccattgat ttacggagta
    63421 acattacctc taggacgccc atttattact gcttgtatcg gtggaggtat cggcggggca
    63481 gtgatcggaa tgatcggaaa tgtcggggct atcgctattg gaccttcagg agccgctttg
    63541 atcccattga tctctgacgg taaatggtta ggctacatcc ttggattatt ggcagcttat
    63601 gctggtggct tcgttgctac attcttcttc ggtattccta aagaccgttt agaaaaagaa
    63661 gaattagcga ctgaaacagc cacagtaaat gaagtgcctg aaaaagtagt acctcaaact
    63721 tcagctgaaa gtgtgactat cctctcatca gttgctgacg gaacagttgt ttctttagat
    63781 aaagcaagtg atccagtttt tgcacaaaag atgatgggtg aaggctactt tgtagaacca
    63841 gaaaacggac atatctattc tccagttact ggggtagtca gctctgtctt cccaacgaaa
    63901 cacgctattg ggatcacaac gccaaatggt ttagaaatct tgcttcatat gggaatcaac
    63961 acagttgact taggcggtac gccatttgat gtgaaagtaa cagaagggca gcaagtgaca
    64021 cctgatacgc ttgtagcaga agctgactta gaagcgatca aagcagcagg caaagaaaca
    64081 tccatgatcg tcttggtaac caatatggat cgtgtaaaaa actttgtctt ggaaaaaaca
    64141 ggtaaagcaa aagcaaaagc acctgtgatg gatgtcaaag cttaatatga tagaaacaat
    64201 agaaacgaaa gaagaaactg aaatctattt ttcagcttct tcttttttat tatttattgt
    64261 tttctgctaa aatctgataa attcgtgtca catcagatgg tgttccccgt aattcataat
    64321 ctgccaagaa aggaaagcca aatgtttgtg catattgttt ggctgttaga caatactgat
    64381 tattgaagtt tttatttccg ctaccgacta caccaagaca aagtttatga ttgtcttcat
    64441 gttccagata ttcacgcatc gtttcggtca agatttctgt atcaccgtta tctaaaccat
    64501 ttccgccgtc taaataagta ggaacgaaag taaaaaatgg ttctgtttct ttcgtaaaat
    64561 cactattttc atggatttct tttaaagtaa cttcaggatt gacaggatct tctgcatgca
    64621 ttttttcagc atattctgct agatgtttgg caaaggcacg cgtatttcct gaaatcgata
    64681 tatataaaat gttcatggat catcctccaa ttctttcctt ttagtatagc atctttttta
    64741 agttttttgg tggtgtaatt tggaaaggga ctaggcgctt tgcttgtata atgagaaaaa
    64801 agccggtaga attatatttc tattaaagtt tttattaatt aagtaaaaat aaaaaaggtt
    64861 tgttatactt tttgataaaa gcggttttat tttggaggat gcttacatgg atacagagca
    64921 acagaaaaaa ggaattggtt taattccact tgctgcctta gtaattggtt cagcgattgg
    64981 aggtggcgta tttggtatta tgacggacat ctccggatcg gctggcggcc cagctatttt
    65041 cagttgggta ctcgtaggaa cagcaatgtt gatgctctcc ttgtcgatca ataatatcaa
    65101 taaaaaacgt ccagatttaa aaggcgggat ctttagttac gcagaagaag gttttggccg
    65161 tttcgctggt tttatcagcg gttggggtta ctggctcaca tgctggttag gcaacgtagc
    65221 ttttgcaaca ttgttgatga gcgcattagg atatttcttc ccagtatttg aaggtgggca
    65281 aaatctggca tcgatcgtga tgagtacagt ctttttatgg ggatatgcat ggctagtgaa
    65341 tcggggagtc gaaaatgcta gtttcgtcaa tgctatcgtc acattctgta aattagtgcc
    65401 attattttta tttattgtag tagctatcag cacgttttcg atccaacaat tcattgataa
    65461 cttttctagc gacattatat tgaatgcgaa tggacagccg atcccgtttt ccagtcaagt
    65521 attgagttgc atcatggtga tgctttgggt atttgttggt attgaaggag cctctgtcgt
    65581 aggttcacga gcaaaaaaac aatcagatgt tgggaaagca acgattctag ggatattcgg
    65641 cttgattatt atttacgtct tggtttccat gcttccatat gggacaatga cgatgaatca
    65701 gctgcaaacg atcaaggcgc agcctgctat gggctatgtg tttgaaatga tggttggtaa
    65761 atggggagca gtcgtgatca acggcggttt gatcatttct ttagttggtg tttggctctc
    65821 atggacgatc ttaccgattg aaacaatggg aaacatggct gaagacggac ttttgcctaa
    65881 agcatgggga aaactcaaca aaaatggggc accaacttat gctatcgtat tgacgacgtt
    65941 atgtacgaat gtctttttac tttcattatt attcactgac agtgcttata actttgctta
    66001 cacacttggt acagcagcta ttttcttcac gtggcttttc ttaggattgt accaaatgaa
    66061 actttcttat cagcggaaag aatgggtcca atttttagta gggcttttag caagtgcctt
    66121 tcaagtatgg gctatgctag tcgtagcctt taatgaagta atgcttgttc ttgttctttt
    66181 cttgccaggc atttattttt acctcaaagc acgtaaggag caaaacgagc ctgtacaaca
    66241 tatccactta gataagattt tacttatagg tgtgacgatg gtcggagtga ttgcaatggt
    66301 tttatttgcc actgggatgc ttcacgtata gaagtataaa agaagtataa agagaggttg
    66361 tgagcctgtc gtttattcaa aaacatacta ggaaaggagc tgtgacacaa atgttgtcac
    66421 agctcctttt tcagaataaa cggtgtttaa gagacagcca atcggcaata aggctgaacg
    66481 tctctttcac agcctctttt ttgatgagtc atcatacatt ttttcgctta aatacgagat
    66541 atcctaatgc taagaacaaa acgatgtaag ctaaattacc gaatagcatt tgattagtag
    66601 aaagatgagt caattgcttc atgatctctt cgtttccaat ttggttttgc agattcaaca
    66661 tactgatcgg attccatttg atccactccc atttttggat gagtgcaata aggataccag
    66721 atatgattga cgaagcaaaa taaaagacga ttcctgctga gatggatgcg ccggaactat
    66781 tgataaagca agcaatcatc aacactaaac ttaaaatcag ccataaaccg atataactac
    66841 ccaaagtata agtgaacaag ctttggatca gtgtttgacc agtagaaacc ttctccgtga
    66901 aggaaatcga agggaaaaga atcagtttca tcaacacagt gacaataata gctaaaaggt
    66961 ataaataaac agaataaata actaatgtga tccatttgct tactaatatt tctcctcgag
    67021 aatactttcg ataaaggaga tttttcaatg taccatattg tgcttccatc gaaatgatcg
    67081 tgcttgcagc tgcaatcatg ataaagacaa tccaagatgt ggcactaaaa agctgagcag
    67141 tcatttcaac tggatcaatg atcgtcgtgt attttttggc tagaagtgct gttcctatga
    67201 gcaaaacaac taatagaaca gataaaacag cagttgactt tttcttgatc aatttaaata
    67261 attcttgttt tacaagcaat gtcatttttc ttcttccccc tttactgaat ccagaatttc
    67321 caatagagaa gattccaaat cattcgtcag atgctggacg tctgtcacat ggatattttg
    67381 tgcagacaat agctggatca cggttcctaa taattcttcg ttttctttta aaagcttgat
    67441 tttatcagga tcttccaaag tatatccagc ttctgtcaag atcgttcgag cagctagatc
    67501 atttgttgta gagataacga tgaaatcttg cgctgtttgg ttcaattcag ccattgtaca
    67561 ttgtttgatg atctttcctt gattgatcag gatcagctca tcgaccattt tttcaagctc
    67621 gctgagtaag tgactggaga tcaagaaaga ggtgccctga gtagccaagt cggtaatgat
    67681 ttttcgcaca tctcttgttg cttgtggatc caaaccgttc atcggctcat ctaaaataac
    67741 gatagacgga tggttcagta aggcaagagc aattcccaat ttttgtttca ttcccaacga
    67801 gtaggatttc gcttttttgt gaatgtactt ttccatcttg agctgattca cgatagtgtc
    67861 gatagcagat acatcgttgg tttcagagaa taacttcaga tgatcatagc ctgttaagaa
    67921 tggatagata cccgggtatt cgatcaatgc tcctacttgt tccaaccctt tatgactaga
    67981 gggagaaatc tcattttcac caatacagat tgttccatct gagtaatgga tcagtcctaa
    68041 aatagctttc atgatcgttg tttttccagc gccattagga cctactaatc caacgatttt
    68101 gccaggggat aagctaaaac tgacatcgct gagggcttgt ttgtgaccaa agttcttgga
    68161 taattcttgg accgaaagac ttttcaaaaa tattcactcc ttttctatag atagtacaag
    68221 catacacctg atgagtggag aagtcgctag gtcattggac ttatttctta ttttttcata
    68281 aaaaaattga atattaaata aaaaggctta ttttttctga tcagtactta ttccaagtgt
    68341 ctagtggccg ctagtaatct tgatgccaat gatactgatg agtagcatcc ccacaaagaa
    68401 ccaagtggaa agattcagct tatcatgaaa caagaccaca ccaattatga ccgaaccaac
    68461 tgcgccaatc cctgtccaga taggataaga gagactcatc ggcaaacttt tgattgcctt
    68521 tgacaaaaag taaaaactag caatcatgcc aatcactgtg taaaaagaat aattgatttt
    68581 agaaaaacca tcactccatt tcatcatcgt tgaccagaat acttccaaaa ttcctgctat
    68641 gattaattcc atccatgcca tacttacatt cctccttaac tataaaaaaa tacacaaaaa
    68701 gaggaaaatc atcccttcaa agacctaatg gaatgatcat cctctttatc ctacccggtg
    68761 gcagattgta tatctttttc tgtagtatag cacacctatc cagatatcgg caatctcata
    68821 ctttttagta aagtgtacca agcactgacg gatatatcgt tagcggaaaa gaagatgaat
    68881 gctttgcaat ttcatagaaa gaagttgtta tttattaatc atttaaaaaa aacagtaact
    68941 agaccttatt tctatatatt atcgggcaat aaattaaaaa aagctttacc ttacaataag
    69001 taagataatt tttgtgataa aaaaataacg aaaaatattt aaaaatagtg tctatttcgt
    69061 gagaaaacaa ctatattttt tatgtgtttt gctttataat taaaaaaata ttgtcatttt
    69121 atggaggaaa atatatggaa aattatctta gccttttagg cgtcttgatt gttattctcg
    69181 ggtttgcatt taaattagac tcgatattga tcgtaatggt tgctttagtt gttacagctt
    69241 tgactggcgg tcttggcatt caaggaatgt tagaactttt aggtacaagt tttgtaaaca
    69301 atcgcgggat ggcgattttt atcatcatta tgttggcaac aggaacgctt gaaagaaatg
    69361 gtctgaaaga atcagcggct tccttgatca agcgattcaa gaaagtatct gctggtatga
    69421 ttgtagatat ttatgggatc tttcgaatga tctttgcggc ttttaacgtg agttttggtg
    69481 gagttgctgg atttgttcgt ccaatcattt taccgatggc attggggacc atcgaatcaa
    69541 aagggttaga agtccatcct aagcatgaag aagaactaaa aggtatagct tcagctatgg
    69601 aaaatgtctg ctggttcttt ggccaagtac tattcatcgg aggagcaggg gcgttgctag
    69661 tccaatcaac actgaaagat ctaggatatg acgtaacatt gggacagctt gctttagtgg
    69721 aagttccagt ggctatcacg gctttagttg tggcaagtgt ctacttctat cttaaagaaa
    69781 agaaattagc gaagaaatat tatccaggaa aggggcaata agatggactt ttttacaaat
    69841 cctgatattt tattaggcga taaattactg gagatccttt atatcgtgat gggactcgta
    69901 ttgatttaca ctggagtaag aaatttaaca gataaaacca atcctcatcg ttatgggtca
    69961 gcctttttct ggacggtctt aggggtagtt attgcaggag gaagatggct tccggcaatc
    70021 gtgaacggtg gcttgatctt tgcgatgacc ataccagcaa ttgctaagaa agtcagcaaa
    70081 ggagaaagcc ggcttccatc aaaagaatat atggaaaaaa tgtcagacaa actaggaatg
    70141 aaaattttta ttccagcttt gagtatcggt gtctttgcga tcttgtttgc tctatttacc
    70201 aatttaggtg ctttagtcgg cgtgggcgtc ggtgtttttg cagccatgct aatcttgatg
    70261 ttcttttctc gagataatcg accaagtaca tttttagatg atgcagcaga tatgctgggc
    70321 acagtagggc cgttaagcat gttgccaatg cttttagctt ctctaggtgc ggtatttaca
    70381 agcgctggag ttgggacagt gatttccaat gcagtaggta cgatcgttcc agaaggaaat
    70441 gtgaacatcg gcatcatcat ttacgctctt ggcatggtga tatttacggt aatcatgggg
    70501 aatgcatttg cggccatcac cgtgatgacg gttgggatcg gtgcaccatt cgtatttagt
    70561 catggagcga atccagcgtt aataggaatg gttgcattga cttgcggttt ttgtgggacc
    70621 ctgctgacac cgatggctgc taacttcaat atcgtgcctg tagcgatgct ggagatgaaa
    70681 gacaaatacg gagtgatcaa aaaccagttg tttattgcgt tatttatgct agttttccaa
    70741 attgcttata tgattctatt taaataatag aagagagggg ttgtgacaga ataattgtcg
    70801 caacctcttc taacattgta gtggaacact atttttttat aagaatatat taagtttgat
    70861 ctgttatgtt atttaaacta ttgtttcctt tatttacgct gttcaaggaa aatggtatga
    70921 taaaagataa ccatatgtta aatcaatcgg acggaggaac aacagatgaa agtactagta
    70981 acaggttttg atccatttgg tggggacaaa gtgaatccag cctatgaagc agtaaaaaaa
    71041 atgccagatg aaatcagcgg tgctgaaatc atcaaagtgg aagtaccgac cgtattcgaa
    71101 aaaagcagtc aagtagtcaa agaagccatc caacagcacc aaccagatgt cgtgatctgt
    71161 gtcggacaag caggtgggcg gtcagcagtc tcgttcgaac gtgtcgcaat caatctagca
    71221 gaagcaagaa tccctgataa tgaaggaaat cagccattcg atacggcttt agaagaaaac
    71281 ggtccagccg cttactttac ctcattgcca atcaaagcaa tgacaaaaaa tgtacaagat
    71341 catggtctac cagcgtacat ctcttataca gcaggaacat ttgtctgcaa tgatatcatg
    71401 tatcgtttgc ttcatttgat cgaaacacaa ttcccggcaa ttcgaggtgg gttcatccac
    71461 gtgccattct cacccgatca agtgatcgat cgtccagtgg gtacgccttc tatgtcttta
    71521 gaagacattg cttcttcttt gacttatgca gttgaagcag cagtagaaaa taaagaagat
    71581 atttccggaa atgccggaac aactcactaa tttagcaaga aacactggct ggttgctaac
    71641 gttatgttag tagccagctt tttgtaatta gaaaggaaag aaacagccgt ggaagaatgg
    71701 caatccgtct ttgaagaatg gtttccaaaa gaaatcagca aaagctatcc catcaaaatt
    71761 tctaaacaat acacaagcag tcaacgttgg gagatctatg caaaattaac gaaaaaacag
    71821 cgggaactag tcgataagca ccgccgatat ctaatcagct ctcgttttat ggaagaacat
    71881 tatttagctg caacagattg ggtgttttct gacttcaaga tcaatccgtt ttttcgaaca
    71941 aaacgaagcc agcaaaaatt gtattgtgag tgtggtagag aattaaaagt gcaatacatc
    72001 gttaaatcgc caaaaaccgg aaagatccta aaactaggca tcaaccactt tgccgatcac
    72061 ctgcatgtct ctccgacagt ggcagcttcg atccatcagg gaatgacaaa agttgatctg
    72121 gccttagacg aattattatg gcttaagcag aagaatatcg attttcctga aggactatgg
    72181 caaaaatact gttttgtctt gtaccaaaat cgtcggatga aacagccata tttaccggat
    72241 ataaaattag ctcagcggct cgcagaattt cgccaagtag aaatgccgat ttacatagcg
    72301 gattatcaag cattggaaaa tgaaatcaag aaaataagtg aacacatcaa tgggcagtca
    72361 aaaaagcgac agataaaaaa agaactattt gacgactttg cagaagaatt agtaaaggat
    72421 gtagaagaat tcttgatcaa ctatcgcgca tttttacgta aagattggca atccatcgta
    72481 tatgaagaag tgccagtaca tcctaatgct tattttgaaa catttatttc cgtgttgaga
    72541 aagacaaaac gtcaacgaac accagaagtc actgctcaga tggaatattt tgcgaaaaac
    72601 caacgattca tccaaccaaa aatctatcta tttatttgga agcaatattg tcgctatggt
    72661 tttacagaag ggttcttcga cagcattcct cggatagtca gaaatggttt tttaaaagta
    72721 ttgcgtaaag aacgagaagc tatacaatct gctgataaga aagaccgtac agtctcaaaa
    72781 gaaaaatggc aactagtagt gaaagatatc caatctggaa atgtgcaaga gacaatcgac
    72841 aaatggaaag gaaaacatta tcgttttact gaagctcaga aacaagcgtt ggaatattat
    72901 cagaagttag aagaatcact gcgtttcaat gatgaggcaa gaaagtatct gaaggaatta
    72961 ctataaaaaa tagaggctgg gacagaagcg tttaactcca agaaataaga agaaattcac
    73021 gaaaattgct tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttctgat
    73081 tccgccgttt atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc
    73141 tcagtttttt tatgcgtgtt gttgtgcgtt gtagtattct tcttccattt tttgcatacg
    73201 ttcacgttct ttttggttat accaaggtga aacggtaaat gcaaggacat cattcagcaa
    73261 gtaaacagag ctgttcacga acatcgccaa acttgcagat ccatgcgtaa atgaaatata
    73321 ccaaaggacc atttgagcaa gccctgaagc taaccaccag taatattggt tgttgtaacg
    73381 caagaaactg atgataccag cagtcaagct aatagaaaaa gcaatggcat ctacccacgg
    73441 ccgaggatca tctgtataac gaccaatcag gaatcctgaa acaagataga caaggaaagt
    73501 actgatgatt gcgataaacc agctttttcc agtgaatttt cggatttttg aagccatatt
    73561 atgattccat tctttactta acaatacggg gatatccaat gtgatcatat aagcaatctg
    73621 ttcaccgata cttaaatagt tctttgctga aaacccaacg ataataaaac aaatcgcaga
    73681 caaaatacct aaaataccgt tgactgattt tgccgcattg atggataaaa tacacaatac
    73741 accaagcgtg gtaccgataa atgtaataac ggacagccaa gtgatgggac tattgacaag
    73801 agtcatgacc tggcagccaa gactgaaaaa gaaaagataa taattttgtt gcggccagcc
    73861 ttttagctga tgagctaaaa actgaaaata tgacttcatg tatgaggact ccttaagaca
    73921 ctaaatgtag tgtgttctgt atttactaac acaatctatt gtatgctttt cttgaaaaaa
    73981 aactagtctt tcaattataa ataatttgtg ttatgataat tttctgtttg ttttcttgaa
    74041 aatataattt cttactttca tttattatat tgggaacagg aagagtgtct tggaggacct
    74101 actttctgtt ttttttgcct tttttcttat aaaaaggaaa aacagctatt tttttcaatc
    74161 tgacacattt acaatggaac tggagattaa tatagatccg agcttaaacg aagaatagga
    74221 gtgctcatat gaaccttaca acgatccatc atatagccat cattgtttca gattatcaga
    74281 aatcacgtga tttttatgtt aataaattag gatttgaagt aatcagagaa aattaccgca
    74341 aagaacgaga tgactataaa ttagatctga aactaggaag tgctgaattg gagatttttg
    74401 gaattgctaa tgcgccacaa cgaccaaact atccagaagc ttgtggcttg cgtcatcttg
    74461 ctttcaaagt agacgatatt gaagaaacaa ttgcagaatt gcagtcctta gggatcgaat
    74521 cagaaccgat tagagaagat acctttacag gaaaaaaaat gacgtttttc tttgatccag
    74581 acggattgcc attagaactg cacgaataaa gataacaaaa ttgtcacttt tttttcgttt
    74641 cattcgattt ttcattcgtt catttcttga tattctttaa aaaaaagaaa caggagaatg
    74701 aagaatgaaa aaaatgatgt ttggtgttgc tgccctcatc gtaataggga ttaccggtat
    74761 gacgggactg ctcaatgccg cttttcacat ggatgatatt agtgaccagg aatatcggat
    74821 ctcattgaaa aaagcagcag caatcgtaaa aaacgaaaca aaagaacata acatatctgc
    74881 tattcatttt gcagttgaaa aagatttgga gaatcctacg aatgcaacat atgaatacca
    74941 gtttttttgt gaaggacaag tcatagcagt caatccatcc actgggaaaa caaccgtacg
    75001 aaatgacacg gaaaaggccg cagaacatct ttttgatata gaaaaaacgg aagagttcaa
    75061 acaaccgcaa gcagcgatga aggaagcagt cagctactac gggaaaaaag caaaagtgaa
    75121 agagtggaca cttctagtca aagatgaacg tccttattat aaagtccgat taatagatgg
    75181 agaacaggac cgtcaggtat ggatatctgc atagaagaag gatattgctt ctctttttta
    75241 caatataaga gtaagctaag agagtaaaga tgaaaggaag ttgagataac atggaaaaaa
    75301 tgaaatgccc taattgcgga aaaaaatttg catacgaaga agtcaataat gtagtcgaac
    75361 ataatgataa agaaatgcca gtcgtctgcc cttattgccg aactgaagcg gcaagaatcg
    75421 ttactcatgg ttatttcatt actgaaaaga ttgaagattt tttaaaataa cgattttgcg
    75481 atttcttttt ctgcatgtta gaatggagaa aaggagagga aactatgatt cgttttgcga
    75541 gaaaagaaga tgctccagaa atcgccccat tgatcttagt gattctaaaa gatatggaac
    75601 tgccattttt gaagcagaca ggggaacaaa agactttaga gattttagaa gaagcaatca
    75661 ctgatccaaa ttatcgttac agttattcac gaggtatcgt agaagaaata gacggtaaag
    75721 ttgcaggagt tgcttacggt tatacagatg aagaagaatc aatcatcgat cagccactgg
    75781 tacctatctt agagaaatat cagctagatg cgtctacacg tttgttcatt gataaagaaa
    75841 cgtttcccaa cgaatggtat ttggactcta tcagtgtatc agaagatttt agaggacaag
    75901 gcatcggttc gcgattatta gaagcattac ctaaattggc gaagaaagct aatcgctccg
    75961 tcatcggtct aagtgtggat gaaaaaaatc caaaggctaa aaaattatac gaacgccatg
    76021 acttcaaagt tgtcggtcag cggatgatca gtggtcatct ttatgatcat atgcaaaaga
    76081 atatctaaaa agatgtacct gaaatgcgta tttccgcatt tcaggtacat ctttttaact
    76141 taagttaagt cggtaagaaa caggcttatc gcgacttttg tctttacggt agagccagta
    76201 atctaaaacc gcagcaatac aaatacaaac tggagcatct tcatcatttg agacatccaa
    76261 tacatagtaa tctcctgaaa aaagtgttgc tttatccatt ttcatgattt gctgattgaa
    76321 atgatggatt tgatattgat gattatagat atcaccttgg actgtccagt gaagtctacg
    76381 aatataataa aagtcaccgg gccaattgaa tattttctgc atggttccca ccttttcgaa
    76441 ctctttatac agctcaaaac gcgtaccgaa tgtcaggctt atctgcttga tgtgagcaac
    76501 gagatcgcca ttcatggcat aaagagacaa tgcatcgccc tttgttcccc agcgaccaac
    76561 catcaaaaac agggaatgtc ctttttcgtt tttgacaatt gtccgagttg cacgtccaag
    76621 ctgcttttcc tgaatgaaaa attctgacat ctatatccct cttttctaga taaaactaca
    76681 agtcttctgc aatcgcgcgc aatatggctt ttcctacgcc agattcaagg tttgtgtccg
    76741 ttgtatattt ggcataatct tttacttcga tttctgcatt tcccattgcg aaactgactc
    76801 ctgctacttg cagcatgctg acatcattga agttatcgcc aatcgtcatg acttcttcta
    76861 aagaaatccc acgctcgtga gcgacatggg caacagcaat tcctttttga gcgtttttat
    76921 ggttcacttc gatattgttt tgaccagagg aagtaacggc tagatcgcct aaaagagaaa
    76981 tctgctttcc aactggacct aatacagctg gcccttctgt atgaaaacaa ataattttta
    77041 aaacttccag attcttttct tccaaaatag cacgaatact gtccacgtat gtgatgtgca
    77101 ataactccag cttagctgag gccatggcaa tcgccatttt atacgtaagg tgaggtaaat
    77161 gggtagccac catggaagca aaactttcaa ttcgtttcgc ttgattttca gaataaagac
    77221 catcgttggt tgctacttca tagtaaatac cgttagcatc tagaatatct aaaacagaga
    77281 ttgcttgatt atttggaatc gacacggtaa acaatgaatt tccttcttta tcaaatacct
    77341 gtgccccgtt caaagtgatc atcgcacaat cgattcctac ctcatctaaa gctgcgcgag
    77401 cttcttgata gtttcgacca gtagcgacca tgaactcaac accggcatca tttgcaaaac
    77461 gtatcgcctc ggcattttca ggagaaacac tcatatgggc atctaataat gtgccgtcca
    77521 tatctgatgc aattaatttg atcataatga ttatcgttcc tttctgcttc ttgttcctag
    77581 tttaccatat taatcaagtg ctgagggtga agattgtccc ttgtgattga agaaaatgtg
    77641 agttattctt tttattagga ggtggagttt ttgaaagaaa tcattcataa cagttggcaa
    77701 gaagttttgt cctcagaatt ttcaaaagat tattatctgc atctaagaga atttttaaaa
    77761 aaagaatatg cgtcccaaaa gatccatcca gatatgtatc atatctatga agcattggaa
    77821 ttgactcctt atgaagaagt taaagtggtg attttaggac aagatcctta tcatggagaa
    77881 aatcaagctc atggcttatc tttttctgtg caacctggcg taaagatccc gccatcgtta
    77941 agaaatatct ataaagagct ttatgatgat cttggaatcg cacctgttca acatggaaat
    78001 ctagtgtcat gggctaaaca gggagtcctt ttactcaaca ccgttcttac cgttcgggaa
    78061 gggcaagcgt attctcatcg cgggaaaggt tgggaaagat taacagacac gatcattgaa
    78121 aaattaaatg aacgggaaaa accaatcgtg tttatcttgt ggggaaaacc ggcacaagaa
    78181 aaaatcaaaa tgatcgataa gagtcgccat atcatcataa catctccaca tcccagtccg
    78241 ttatctgcaa gtcgtggctt ttttggttcc aaacccttct cgaaaacgaa tgatgcgctc
    78301 cttgcgttag gagaagaacc gattgattgg caactgcctg aaacagtgta ataaaacagc
    78361 gctttcctgt tatagtgcag ttcgctttgt atgtgaaaaa cttaattttt ccagcatttt
    78421 tgactgatac agataaaata aactgttctt tttttctgga atagtgtatg ataatacata
    78481 taaaaacgtc tggaggtcat cttgtggaat tgtttgatag tttaagattt aaaattgttc
    78541 gccgtgggat caaaatcgtt ttcccggaag ctactgatgc tcgtatttta ggcgcagctg
    78601 cccggttgaa agcagaagaa cttgtggaac ccattttagt agggaacgaa gaagaaataa
    78661 gaaatgcagc gcatgccaga ggaatcaaaa catctagctt tacgatcatc agccctgata
    78721 attacgataa gtgggatgaa atggtggata cttttgttga acgtagaaat ggaaaggcat
    78781 ccaaagaaca agcagaaaag attttaaaag atgtgaatta ctttggtacg atgcttacgt
    78841 atatggggat tgcagacgga atggtctctg gagcgatcca ttcgactggc gacactgttc
    78901 gcccagcact tcagatcatc aaaacaaagc ctggcatcag tcggacaagc ggagcgatga
    78961 tcatggtacg tggaagagat caagaaaaat atgtcttttc ggattgtgcg atcaacgtga
    79021 atccaactgc gcaagaattg gctgaaatcg ccgtagattc tgcaaaaaca gcagaattgt
    79081 tcgatatcga accaaaagta gcaatgatga gcttctcgac aaaaggctca gcgaaatcgc
    79141 cagaagtaga caaagtagtt gaagcaacta aaatcgctaa aaaactagcg ccacaataca
    79201 tgatcgacgg cgaattgcaa tttgatgctt cttatgttcc ttcagttgca caactgaaag
    79261 cgccagattc agaagtagct ggtcaagcaa cagtctttgt atttccagaa ttgcagtcag
    79321 gaaatatcgg ctataaaatt gctcagcgtt tcggtaactt tgaagcaatc ggaccaattc
    79381 tacaaggctt gaacaaaccg gtttctgatt tgtctcgtgg agcaaatgaa gaagatgtat
    79441 ataaattatc gattatcaca gcagcacaaa cattgattga ttaacaaata cacgcgaact
    79501 agccaaaaac agcagctgct gttcttgact agttcatttt ttctttaaaa aaacttcatt
    79561 acgcgatata ctggcaaaaa gaaagaagag cgggagaaga tgaaaaatga tcgaactatc
    79621 aggattaaca atgacagaac aattcgcaga ggcaattggt cattccgcaa tgccaggtga
    79681 caatctgata ttgactggtg atctaggtgc gggtaaaaca acgttgacga aaggaattgc
    79741 ccaaggatta gggataacgc aaatgatcaa aagtccgaca tacacgatta ttagagaata
    79801 tagtcaaggt cggattccgt tgtatcatat ggatatttat cgggtagcag ctagcggagc
    79861 agatttaggg ttagatgaat attttgaagg tgatgggctt tccgttgttg aatggggaaa
    79921 tctattagaa gaagcactgc cggaggatta tttggaactg attttagaaa aaagtgatac
    79981 agatttagaa aagcggtatg tgaaactaat ctcatacggg gagcaaagtg aaaccttcaa
    80041 acagcgaatt ctagaaaaat gggctgaaaa tcatgagtga agaagttggg atcatcttgc
    80101 gggaagcagt gccaggagat gcaaaagaca ttcttttgat gatgggacaa gttaataagg
    80161 agacagaatt tttagttttg gatgaagcag aactcctgtt accaccagaa accttagaag
    80221 aagagttgga ttatatatac gaaagcaata ataatttatt gctgcttgca atctatgaag
    80281 ggacgattat tggaacagca tctgtcaagg cagacagtca atttcgtttg tctcacgtgg
    80341 gagaagtcgg tatcagtatt ctgcaggaat attggggaat gggactaggc actcttatgc
    80401 tagaagaaat catcagttgg gcgaaggaaa tgggtgtact ttttcgattg gagcttgacg
    80461 ttcaagttcg taatgaaaga gccgtacacc tttaccgaaa aatgggcttc caaatcgaag
    80521 cggtcatgcc tagaggggca agaacagatc taggtgaatt tttagatgta tataaaatga
    80581 gttatttgat tgaatagaag aaataggaaa agagtgagaa actgtcatcc ttttgagaag
    80641 gcagaaaaac acacgaaggg actgtgacaa aagtcgtgtc gcagtccatt ttttttgaat
    80701 aaacggtgtt caatcagctg accttcggta ttagctcaaa aaacggcaaa atatgaggaa
    80761 ctattttgcc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttgg
    80821 taaacggtgt ccaaaagctt gcatctgcgg taataagtcc aaccaaatga aaaatatgag
    80881 aagctatttt tgtttggctg ttcttattac ttgatgcaga acagcttttt cacaacctct
    80941 ccaataaacg gtgttctcct gccagctcct cggtcaccag tcacaatatt taataaacat
    81001 gagaagctat tttttgaaat tgctccttgt gactcggagt gaaacggcag gctcacgacc
    81061 tctttgtgtg tttcataagt attcttttac tttatagtca caagtttttt caatggatct
    81121 gtcccgaatt ttttttcttg ataaagcagg atttccgcac atgcacgact atcttccaat
    81181 gcatcatggt gatgatctaa ttggatgttc aaattttcgc acacagtgtt caatcgatga
    81241 tttggaaatt caggaaacag gcgacggcta cttttcactg tacataaaga aagataatta
    81301 ggctgttcaa gatgataata atctaagcat cccgctaaga cctttgtatc aaaagcagca
    81361 ttatgtgcaa cgactaaacg atttttttga aaatatggtt ggatagtttg ccagacttct
    81421 gggaatttcg gcgcattgcg tacgtcttct tgatggattc cgtgaatttg gacatttcgc
    81481 cagaaaaaag gagtctcagg ctgtatcaat gtatagaatt catcaacgat ctggctattt
    81541 tttaccatga ctagcgcaag agaacaagca ctataaggct gatgattggc agtttcaaaa
    81601 tccatggcaa caaaattcat ttctgtaaaa cctccttggg aaattactgt aacatacttt
    81661 tgaaaaaagc aaataaattt aacaaaaaac atagaatttt ttatgtaata tttgttgaga
    81721 aaagctagtt tttgaactca ttccttgtga tttggagtta gatggggatc atgacttctc
    81781 ggtattaagt caaatttatg caaagttgtg aggaaccatt ttcaaaaaaa tttcttacga
    81841 ctcgaaagta ggcgactttt tcacaacttc tccactaaac ggtgttcaat cagctgaccc
    81901 tcggtattag ctcaaaaaat agcaaaatat gaggaactat tttgccattt gtttctctaa
    81961 tactcaggtc aggacgctga tttcacaact tctcaataaa cggtgttcaa aagcttgcat
    82021 ctgcggtaat aagtccaacc aaacgaaaaa tatgaggaac tatttttgtt tggctgttct
    82081 tattacttga tgcagtgcag ctttttcaca acctcttaat aaacggtgtt caaaagcttg
    82141 catctgcggt aataagccca accaaacgaa aaatatgagg aactattttt gtttggctgt
    82201 tcttattact tgatgcagtg cagctttttc acaacctctt aataaacggt gttcaaaagc
    82261 ttgtatctgc ggtaataagc ccaaccaaac gaaaaatatg aggaactatt tttgtttggc
    82321 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctcgataaac agtgttcaaa
    82381 agccagtttc tcggtattat gccaaattta tgcaaaatgt ggaaagccat tttcacaaaa
    82441 tttttcttaa tactcgaaac taaacgactt tttcacaacc tctttatttc aaatctaatt
    82501 ctactggaca atgatcactg cccataatgt ccgtatggat ctttgcatca gctagtcctg
    82561 gctctaaatc tttgctcgtc aaaaagtaat cgatccgcca accagcattg tttttccttg
    82621 aattgaatcg gtagttccac caagagtaga caccttcctg ggttggataa aaataacgga
    82681 atgtatcgat aaacccgtta tccaacagtc gagaaagagc cgctctttct tctggagtga
    82741 atccagcatt tttttgattc gttttccagt ttttcaaatc aatcttttga tgagcgacat
    82801 ttagatcccc acacacgatg actggttttt gtttttttag attttccaaa taattataaa
    82861 aggcttcttc ccattctaat cggtaatcta aacgttttaa ttcgctttga gaatttggcg
    82921 tgtaacaggt tacaagaaag aaatcggaat attccaaagt aatcagacgg ccttctgtat
    82981 catggatatc gatacccatt ccgtaagtgg cattcaatgc tggtttttta gcaaaaatcg
    83041 ctgtgccgga atagcctttt cgttccgcgt aattccaata ttgataataa cctggaagat
    83101 ctaaatcaat ttgtcctgct tggagtttcg tttcctgcag acagaaaaaa tcggcgtcta
    83161 actcatgaaa aacttctaaa aaatttttat ttacgatggc acgaagcccg tttacgttcc
    83221 aagagataaa tttcaataaa agcacactcc ttcgaattaa tttccttacc tttgcgatac
    83281 tattgtacct cattttagta aaaaatggtg taagatgaaa gtaactaaat gatccaggag
    83341 gaaagtagat gaagattggt gtaatcggtg tagggaatat cgcagaaaaa gcatatttac
    83401 caacttatgc gaaaaaacaa ggaacacttg atttttattt tgcaacaaga aataaagaaa
    83461 caaaagaaag aattcaaaaa gagtacgggt tccaacatct atatgaaacg atcgatgagc
    83521 ttttagaaga aaaaatcgaa gcatgtatga tccacgcagc aacaaaagta cattatgaat
    83581 tagccaaaaa atgtctagaa aatggtatcc atgtttatat tgacaaacca ctgagtgtcc
    83641 atctaaatga agttcgtgaa ttgcaagaat tagctgataa gaaccaaata attttgatga
    83701 tcggtttcaa ccggcgcttt gctcctatgg tagaagaatt gaaacgtatc ccagataagc
    83761 ggttgatcca cctgcaaaaa aatcgaatag cggcaaaaga aacgacagag ttcgtccttt
    83821 atgatttgtt tttacatctt attgatacag ctgtgtattt attagatgaa cccattttga
    83881 gaacaaaata ccagataaga gaaacaaagg aatttttgga atatgccatt ttacaattag
    83941 aaacacaaaa tcaaacggct attttaacga tggatttagc aagtggcgct aatatagaaa
    84001 aatatcaagt aacaagtaaa aatggtactt atgaagtaaa cgatctgacc cagcttgtca
    84061 ttcaagaggc tgatggaaaa aaagtgaaag aatttggcga ctggacaaac acgctagtca
    84121 aacgcgggtt tgagccaatg gtagaggcct tcttttctca gatccgttta ggtaaatcgg
    84181 aaacagaatt gcttaaacaa aaagaagttg taagaagtca tgaattatgt gaggaagtgc
    84241 ttaaagatca ctaccgtcat caactttgaa caagactggc aagattgtat taagcgtgat
    84301 agaataatga ggacgtttga gaaaagaaag gaaaatcctc agtgaataaa gaagaaatag
    84361 ttgaaagctt agccacgatt actatattgt ttgatgaacc attgatgaac tatacgttta
    84421 cgaaaacagg tggaccagct gatgtccttg cattcccgaa aaaacaagaa gaagtaaagc
    84481 aaatcatcga ttattgccga atccatgata taccttggat ggtattagga aatgcaagca
    84541 atctgatcgt tcaagatggc gggattcgcg gtgtagtcat catgctgact gaaatgaaac
    84601 agattcatgt caaaggaacg atggtcattg cggaagcagg tgcctcactg attgatacga
    84661 cttatgcagc tttggcagaa tcattgactg gatttgaatt tgcttgcggc atcccgggaa
    84721 gtgtcggtgg agcggtttat atgaatgcag gtgcgtatgg cggagagatc aaagatgttt
    84781 ttgctgaagt ggatcttttg cttgaagatg gaacattgaa aactttgaca aaagacgaaa
    84841 tggtattttc ttatcgtcac agtaaagtcc aagaattacg tgctatcgta ttggaagctc
    84901 gattcagttt gcaaacaggg gattatgaag caatcaaagc aagaatggat gagttgaccg
    84961 aattgcgtca atcaaaacag ccattagaat acccttcatg cggaagtgtc tttaagcgcc
    85021 cagtaggaca ctatacaggt cagctgatcc aacaagcagg attgcaaggc ctgaaatggg
    85081 gcggtgcaca agtctctgaa aaacatgctg gattcatcgt caatatcgat catgcgacag
    85141 cgacagatta tattgaattg attgcgcaca tccaagaagt aatcaaagaa aaatttgatg
    85201 tgtctttaga aacagaagtg cggatcattg gagaaaaaca agcaatatca gaaacaagca
    85261 gaagtttagc caattaataa gtaatggaac ttaaaataag taaaagagca ggacggtggc
    85321 cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca aatcttgtac attttaacac
    85381 aataaaacaa aaaatataga atatttcctc ataaattttt ccattttttg caatattctc
    85441 aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca agcataatcc atcataaaat
    85501 cagtataggt gaaaagatga actttcatct gcatatcaag taattggtat gaggcatttt
    85561 ttgaggtgta ggatatgaag ttggattacc ttatagaaga ggaagataga gaaaaactgc
    85621 tgctagctag atatttagaa tttgagaaaa agaattatgt agtgctgcct aagttatatg
    85681 aaacattagg tttgtctaaa tttaaaacaa gaaattatat catagaatta aataaagaat
    85741 ttactgaatt caaaacaaag ccagagatcc aaatttcaga agaagcagag atccatgtcg
    85801 aacgtctgaa tttgacggat atcaaaaatt taagaagaat ctactttcaa cgatcaaaaa
    85861 ttgctcgact gttaacggag atcgtggatt caaaagtaac gatcgaaaaa tttgcagaga
    85921 atcatttttt aagcatatca agagcctatg taaaaagaag agaattagtc tcttttcttg
    85981 aacagcatca aataaagtta aagaaaaaca tattagttgg ggaagaaata aacatacgga
    86041 acgtattatt tggggtatat ttcggggtct ttaatggttt ttctatgcca tttagagaaa
    86101 gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga atatctgttc aatttaaaac
    86161 tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac tattagtttg ttgagggcct
    86221 atgggaatca accaatcgga aaacgtttct tttcagatga atactttaag agctcagaag
    86281 gaatgaagat catcaaggat ttatcacata tttcttttct tcatatccct aaagagcaga
    86341 tcattgatga gatttcttat atcatgttgt ttttgatcac ggaagaagaa tttgaattag
    86401 atggcgcatt tggacttgcg gaagctcatt ttcatgatat ccacatgatt agtcaaaaaa
    86461 tgtcagtgga attgattgat caattaccat tttcgattga aatctctgaa gaagaaagac
    86521 tatatatacg aaaagaactt cgtatgggta tgatgcatgt caatcgaaaa aattggatat
    86581 tcgactttga gatgactagc tttacgtcta aaaagcagct acagtttttc ttcgagacgt
    86641 atccaatatt cagtacagca atccaaaagg tcatacaaga gaataaacgc tatttcttgg
    86701 gaaaagatga acgtttcatg attcgcttat tttatgatta tctcttttta ttggtcggta
    86761 tttgtccggt atcatatttt gaagcaccga ttcatgtctg tattgatttt tcgcaaggat
    86821 ctgcttatac cgattacatt atttctcaag tgaaaggatt taaaaattac aatattgtga
    86881 ttgagagtcg aatcacttct cgtacgaata tttatttatc tgattgttta cttgaaaaat
    86941 ttaataaaga acagataatt tggaaaaatc caccgactcc agatgactgg gaatattttg
    87001 gaaataccat catcagagaa aaaaatagac atttaactga aaaaatcagc tagtatttat
    87061 ggatggcagc tttgtctcac atatttatta gctgatttat gtttttttca aagaaaaggt
    87121 aactagatat aactagatat aactagatat attacttaac aaaattaaaa agtacataag
    87181 ttattattcc gattttcttg aaacaataaa aaatcaaaaa aaatactctc ctaatagtcc
    87241 atcttctttt gattttattt atataatgaa atcatcttat gaaaattact acttcatgtt
    87301 tagacaagag tagggaggac taagaataaa tgtgttcaaa aagacgttca aaacaactaa
    87361 ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc tgaaaaacac tacgttaagt
    87421 ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt ctatatgcat gtacccgttc
    87481 aggcaacgac acaaaccatc aaggtcgtac gtacctctgt ttctgagacc actgattccc
    87541 ctatcactat tactcatact ttcactgatg caaatggaaa acagctactt cctgcattga
    87601 cttcctataa taaagtcagt tatcctccaa cgataaacca ctactggtta attcctaaca
    87661 aaacattatg gacatcggaa accggaagca gtgtgccgct ttctatcttt tccgatatga
    87721 ctgatcctac aaaaggtgat cagttaaaac agatcactga tattttgaat catgctttga
    87781 cagatataaa ggacggtgga agcgcacaag tttcttatgt atatgaaaaa gattattcac
    87841 agtttgaaag aaagaaaatc gatgttctgg cagggacaaa agtttcgata tctgatctgg
    87901 tagaaaggct gaagaacgca gatggaacac aaggagatat gtccaaagta atttcaaaca
    87961 atgggaatat cgtggatacg actaatacag gaacatctac tatttccttg tcctattttg
    88021 atccaattgc tttaaaaaca atgactgcat ctgctgtgat ccatgttcgt gctgatcaaa
    88081 cagctatcaa aggaaaagat cttcacttaa gaacaggaga gagttacgcc cctaaagatc
    88141 ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa taaagaagaa atcaaaatga
    88201 acgggacagt agatactaca actccgggag aatatcccgt tgtattaact tatatagact
    88261 cgaaaacatc aaatgtggca gaaacaacag ctgtagttta tgtagaagca tctgctgctt
    88321 catcgactgt catcactcat tttatagata gtcaaacaaa taaagaactt caagcagaaa
    88381 cacatatgag tagtgtaaat cgtattcctg atttgcgcct acctgctgaa ttttctgatc
    88441 gagtgccaaa tatagattta tcgacttatg aacaaaatgg gaaagtcact tctatttctg
    88501 attggatgca agcaaacgga ttagacagaa aaagtcactg gtcggagatc cttgaagtac
    88561 ttcactcaga actgatgaat aataggaagc aatcaggagg ggaaattacc tttacttatg
    88621 tttatatacc agacaatcgt tcattagaag gtcatgatct tattgttcat ccaaacgaaa
    88681 aaataaacaa agaagagttg atccaatcag caacaaattc cttaggtaaa cccgtaccgt
    88741 taaaggatgt atcggttaca atcaatgaag taccattgga ggatggttat ttgaccaaac
    88801 aaacaggtga ttttacgatt atgtatagtt atcatgaccc gtattcactc caagaactcc
    88861 atgctcagtc actattacat gttttaccat taaacaaaca gatagatcaa aaagaacaaa
    88921 aaagacgaga agaaagcttg tcagaaaaaa aacaacaaat ggctcatgca gtttctccaa
    88981 cagggaaagt aaaacaaaca agaagcctag agttagcaag aatatctaga aaaatctcag
    89041 aatctaataa aacgataagg caagaagctc atttggcccc acaagtttct ggattggaaa
    89101 atgaaatttt agagcctaaa ataaaacaac aatcagaaaa agaatttgtt gctaaacagt
    89161 cggaagatca gaaggaatca gatgaccaga aggaaacgaa gaaaaaacgc ttgtcaacta
    89221 ttgattccac cgatgagcat ctgcagccgc aagtaccact tcctagtccg ggaacacccg
    89281 cacctggtgg aggtattcct tcttctgctt taggagattt catgcgaggt atggcaggaa
    89341 catgggtata cgctaatcga aatgaatttg acgcagatga ctggttctga tttatatctc
    89401 tatctgttat agtcaaataa gtagctgagt catatgttat ttggcataat ggagacgagg
    89461 agggatagaa aaatgttaga taaaacgtta ccttacgcag aaatctggat gacaagacca
    89521 cttcaacaaa atgttccaat cacgccttta gcggaaggtt ttgaatttcg attttatcaa
    89581 aaagaagatg aatctgcttg ggcggagatt gagacttccg ttggggaatt tcaaacgaca
    89641 gatgaagcac ttgagtattt tcaacaaaac tttgctcctt atcctgaaga acttgaaaga
    89701 aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag caacctgtac cgcttggcga
    89761 aaaaaatcaa gagagcaagt gtatccggtt tttcattggt tagcagtgaa accagattac
    89821 caaggaaaag ggctcgcaaa agcattgact gccagagtct tgcaagaatt tcctaccctc
    89881 catacggacg gaccaatcta tcttcacaca cagacatgga gccatcaggc gattgctctt
    89941 tataaaaaaa tgggttttac ttttattgct gaaaatctag acggcacacc taatccagat
    90001 tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat aacgaaatgg tgaaataagt
    90061 cctcagtatt gggataaaaa acgaaatagt cattcctcat atcttacttt tttgagttaa
    90121 tactatcgga taactaatgg catgccatac attcaaaaaa gaaggggcag tgacaaaagt
    90181 attgtcactg ccccttctct tgttttttag catgttttat cgtttgattt ttttaggaat
    90241 aaaattcttg atgaacgtaa atttttggta atcgtttcga ctatagaggt aaccaaatac
    90301 actgaaaaca actgctccaa taaaattaac aaataaatcc ttcatagtat ccatgattcc
    90361 cacgtctaag taaccatttt ggatgattgt tttatctgtt ttaccatctg cattttgact
    90421 ttcaatgatt gtccgagtga tatctggaac atgatagaca gtgtttccgt ctttacttaa
    90481 ctccacacta ttgatttgct ggatgatcgt atctttttgc atatcgaatt gcagtaattt
    90541 atctgctgaa aattccacaa actcccacat aacaccaact gtcatcgaaa aacagaagga
    90601 aacaactgct agtaataaag gagtcaagtt catggttttt gtatttttat tgagcaaata
    90661 aacaagggaa aagccgacac ccccagctaa aaatccgctg aatgcatgta gaaaagtatc
    90721 ccacattgaa aaatagccat aaaaatcact taactctcct aaaactgttg aactaaaaat
    90781 aaaagataac aaaatgattt ctagcatatt tggaatctgt atctttaata tcttttccag
    90841 aaaataaggg atttcaaata aaattaatgt cagtaataaa agcattgtat ttccccagtg
    90901 ttggttccag acctgatcca ctagcataat aacgacgatg atacgtaata aaatatagat
    90961 tccgtttgtg attctacctg cagtttttgt actgttcgta aaaaaggttt tcaaatgatt
    91021 cataaaataa ctccttcatg tatatcctta attatactaa gaagcgtgtg tttaccaaag
    91081 ctatatgact taaagaaaaa cagagttgat attgtgaaaa aaatcactta atatagtata
    91141 aggagaaaag ggggatacaa atgaaaaaat tagttgctgg tttattaaca gccgttgcac
    91201 ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac agcgcagaca agttctagta
    91261 cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca agtatataca gatccaaaag
    91321 aaatgaaaga atcttatgat gtcgtgatta ttggagctgg tggtgcaggg atggcagcag
    91381 ctatcgaagc aaaagatgct gggttgaatc cagtaatttt ggaaaagatg cctgttgccg
    91441 gaggaaacac atctaaatca tcaagcggta tgaatgcttc agaaacaaaa tttcaaaaag
    91501 aagaaggaat caaagactcg aatgatgcct tctatgaaga aacgctaaaa ggtggtggag
    91561 gaacgaatga taaagaaatg cttcgatact ttgtcgatca ctctgctgat gcggtcgatt
    91621 ggctagatca aaacggtatc aaactggaca acttaacgat cactggaggg atgagtgaaa
    91681 agaggacaca tcgtccgtct gatggctcgg ctattggtgg atacttagtg gaggggttat
    91741 tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa cgcagatgtg acagacatta
    91801 agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt tcaaggagaa gaaccaaaag
    91861 aggtcaccgg aaaagcaatc gtagttacca caggcggttt tggtgccagc aaagaattta
    91921 ttgaaaaata tcgtcccgat ttagctgatt atattacgac aaatcaagaa ggatcaactg
    91981 gagatggtat caccatgatt gaaaaattag gtggacaaac cgttgatatg gataaaatac
    92041 aaattcatcc aactgtgcag caggatgagg gggtcttgat tggtgaggtt gttcgcggag
    92101 aaggagcaat cttggtcgat caagagggga aaagatttgt caatgaaatg gatacaaggg
    92161 ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc agcttatttg atttttgacc
    92221 aaggcgtgcg tgatcgtgcc acagctattg aattttatga ccaaaaagga tatgtcacaa
    92281 ctggaaagac aatggaagaa ttagcagaaa aaatcgattt accaaaagaa acactagcaa
    92341 aaacagttga tggatggaat acggatgttg cggagaaaaa agatgctcaa ttcaaccgtg
    92401 ccacagccat ggaacatgac ttatcgtctc cgaattatta tgcgatcaaa atcgcaccag
    92461 gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa cactgaagta ttagataaag
    92521 aaaaccagcc aattaggggg ttatatgctg ctggtgaagt cacaggtggc ttacatggaa
    92581 acaaccggat cggtggaaat tcagttgcgg agatcattat ttttgggcga caagcgggca
    92641 tacaagcagc tcgatttgct tcggaaaatg aatgaaatag aacgtaaata aaaagaggtt
    92701 gtgaaatcag cgttttgagc taataccgag ggtcagctga ttgaacactg tttagtgaag
    92761 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct
    92821 tatgttttta acttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    92881 cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    92941 aaacggcgga agcagaagaa attccttcgg aaataagctg aaattcacaa aaatttgaaa
    93001 agcaattttc gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    93061 ttttgatcag catgatttta ttcagttatc ttcaaaatga tttttccgat gtttttgttt
    93121 gcttccatat attcatgcgc ttgtttgact gcgctgaatt ggaatgtacg gtcaatgatt
    93181 ggccgaatct tgccattttc tagaaaaggc ccagcttttt gcataaaagt ttgtgcgagc
    93241 tctgctttat actgatcact tctaggtgtc aataatgtac caagcagttg gacacgtttc
    93301 ccaatcaata caccaaggtc tatctcaggc acgatggtcc cacctagcat accaatcaag
    93361 acccaacgtc catcaacggc tatagaagct aaattctttt cccagtaaga agcgccgatg
    93421 aaatccaaaa taacatcgac tccccgacct tcagtgattt ttcgtacctc ttcttgaaag
    93481 tcttgttttt tatagttgat caattcatct gcaccaagtt ctttacaaaa ggcgagtttt
    93541 tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa gctgtctagc taattggatc
    93601 gctgctgttc ccacaccact tgctcctgca tggatcaaga cagtttcgcc ttcttgtaat
    93661 tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa cttctgagat tccagcagca
    93721 gaaacatagt ctagcgtatc agggagaagg attgcacgat cggcaggcat cacagcatat
    93781 tcggcataac cacctagatt gactaatccg tatacttttg tcccaggagt aaaagtggag
    93841 atatcacttt tattctcaac tacctctcca gctacttcga ctcccaaaat agggtaggga
    93901 ggtttcaaag agactgattc tcttttcata atatctgttc gattgacagc tgctgtatgg
    93961 actttgatca gtaattcgcc ttgttttggt ttaggaacag gcacttcaat ttgttctaat
    94021 acatctacag aaccaggatg cttgattgaa atggcacgca tggatctatt cctcctttgc
    94081 ccattcttgg aatgctgtat tcaactcttt gctaccttca gaaagtacta gagtcgtacg
    94141 agaaatatcc gtttcttcca ctagagcggc aagtacagca gcaactgtag agcgagtgat
    94201 cgtttttgat gccttctcat taggcttagt taattgaatg gaagtaactt cttcatcgtt
    94261 agtcaatgtg actggttgaa gaatgacaaa atctaagtta gttcgtttca gccattcatc
    94321 tgcataatgt ttagtaatat aataatctgt catggactca ggccattttg ctggttcatc
    94381 tgcataaaca gcactgacca tcagatagcg agagacatgc gcattttcag ctgcgatcac
    94441 tgttttgatt gcgccatcca agtctacttg cagaagattc tttccttgag agccggcagc
    94501 aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt ttttccacgg accaagttaa
    94561 atcaaaagag acatacgaaa cattctcttt ttttactaat gtttgggaag caacatctct
    94621 cacccctgca aaaatttcat gggaacttgg agctaaatct tttatcaggt gtcggccaat
    94681 ttgtccgttt gcacctacaa caaaaatttt catttggatc atcctttcga cgtcaaatct
    94741 ttccattcaa tagtaacacc aaaagagaaa aaaatcagag atcggctacg atgactatca
    94801 gttagtaaga gaagggtgct tgaagtatct taacaagaca ttgcatactg ctgcagtcgc
    94861 aagacctgcg acgattcctt caggtatacc acttgtaaaa ataaccgcta gaatagctga
    94921 ataaacagca tttaaatttg ttccgatgac tttggcataa tcttgctgga ataaaaagta
    94981 gatcatattc atcactaaaa tcgtattggt caatgaaccg gaaagtccag ctaagaataa
    95041 agaaacggaa tctgcttttt gtttcattag tttgcgcaga cctttataaa cgaaataagg
    95101 aaccacccca atcaagatgc gaggaataaa cgcgatcagc aaggctttcc agcttccatg
    95161 ttctgttccg atgactggga taaacggaga aaagacaaaa gacaaaggtg tttggataac
    95221 tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca agaaaagcac ctagtcttgg
    95281 tcctaaaacg atcgaagcaa tgattacagg aatatgcatg gttgttgcat tgattggacc
    95341 gatagggata aaacctaaag gcgtaacagc taataaaatc aaaatagcta aaaacatcgc
    95401 agttaatgta aagttttttg tatttttcat aagtactcct tttttctaag catttctttc
    95461 attaagagtc agcatgacct gttcgatgat cgtctgaata tcagctaagg caccttttcc
    95521 aaaatctcca caagctaaaa gagattctct tggttcaatt tcctgatagc cgacagatct
    95581 caatgtttcc agattacgct gagtgatcgg gttttgatac atattcgtgt tcatggcagg
    95641 ggcaatcaat tttggtgttc ccactggaag cgccattgcg actgttgaaa gaagatcgtc
    95701 tgcaatgccg tttgctagct tgccgataat gtttgctgta gcaggcgcga tgagaaacaa
    95761 gtcagcttgt tttgctagtt cgatatgatt gattacctgc ggatttgatt cttgcatgac
    95821 gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt aatggcgtga taaaatgtgt
    95881 gctgctttta gacataatca catctacctg ggcacctaat ttgacgaatt gacttgtaag
    95941 atcagcgctt ttgtaagcag aaatacttcc agtaacgcct agtaagatct ttttcatttt
    96001 gattcctcca gtctaatttt ctcagtaatc aatgcagcaa tctcagattt actttgagct
    96061 tcttccactg ttccatcttt agataagaga tagccgtggt gtgtctcgcc atggatttcc
    96121 gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta tcagactatt ttttgcgact
    96181 gccagaagag attcctttgg tacatctact aatagtttga aaccaatgat ttttgctttt
    96241 ggctgccatt cacgtagata agaaatgatt tttggtgttt tcttcaaaac gagcacaaga
    96301 tgctcggtgt tagaagagat ctttgaaaca gtggtatctg tctgttcatt ttccgcaaac
    96361 caattgtcca gttcttcttg cgttgaagaa gtgggtaggt tttttgccag ctgttcttct
    96421 gaaaaactaa aagcaggagt aaaatcactg actgccatac tgtggataat cgcatcgtag
    96481 tgttcttttt tcgttagttc ttctaattgt aaaaataagt ccagtgcaga ttcgatcgta
    96541 taaatcgtta tattggagtg tcttttcggt ttcagtgctt gtttcgtagt cacgtaatcg
    96601 attgtggtat ccggattggc agctaaataa tcagctaatg cttgccctaa tcgtcctgtc
    96661 gaatgatttg taatagcacg tacttgatcg attttttcag tcgttcctcc agctgtgatt
    96721 agaatcttca tttatggtca cctacattta ctagtgttct tccttggtgt gttccagcaa
    96781 gtagtttaga tgctgtttca ggcaattgat ccagagtaat ctcttggacg accagttcat
    96841 ctgcaatctg caaatccgcc atctgttgcc ataaagaaag acgttgatct attggtacat
    96901 tgacagaatc aattccaatc aactggatgt ttcgcagaat aaaaggcaag acagtcgcgt
    96961 ttagtttgat tcccccagca ttgccacata aagctactgc accgttataa gagatcaatg
    97021 gaagaagact gcttagctgc tcgccaccta ctgtgtcgat gacatagtcg atttgctgtt
    97081 ttcctaaagc ttttgttgtc tctggcagaa attcatcagg tcgtacgact tgcaaagcac
    97141 ctagactttt tagccatgtg acagactctt cttttctgga aaaggcaatg attgactgga
    97201 atcctagttt atgtaacagg gctatcgctg tactgcctac accgccagaa gcaccagtaa
    97261 cgacaacttt tttatcggag gtcatacctt gacggattaa agcgttcaca cacaacatag
    97321 cagtaaaccc tgctgtacca aggatcatcg cttgacgcag cgtcatattt tttggtagag
    97381 gaactaacca gtctcctggt actttttgat attggctata accgcctgga tgggtgacgc
    97441 caagcccaaa accagtagca agtacaagat cttcttttga aaaacgtgga ttgttacttt
    97501 ggacgacagt tccggataaa tcaatccctg gagtcatcgg atatgttcgt atcacctgac
    97561 cagactcgct cattgccaat gcatctttat aattgacatc agaataggca atttttacca
    97621 agacttcatt ttcagccaac tcagtaaatg gcagatcact gattgctgta tggaaaacag
    97681 gttcctttat tacctgaaaa gatcgaaaag cgttcattcg tgagacactc cttttaaaat
    97741 tttcttttat cataccataa agcctactct tttattcttg ttcttccgta aatttagaca
    97801 aaaattaggt gaaaaataaa gaatcaaaca actagaattt attccaaaag ataaagaaat
    97861 attcagcaaa aaaaatttta caaagggttg cctttttcaa taaatttcgt atactaatac
    97921 gagtggtagt aaacaggaag tttactatta gaaaacttcg attcggagga aattatggaa
    97981 attggtgaga agttaagaaa tttgcgcatc caaaagaatt tgacacaaga agaactaggc
    98041 gaacgcacag atttgtcaaa agggtatatt tcccagttgg aacgagattt aagttcacca
    98101 tcaatggaaa cattcttttc tattcttgaa gtcttaggtg tgactccaga agaatttttt
    98161 catcaagaag cagcgaatct gcaagtggtt tattcaaaag aagatcatac ggtttatttc
    98221 gatgaagaaa acgggtatga attggaatgg ctcgtggctg attcgaatga aaaagaaatg
    98281 gagcctgtct tgctgacttt tgaaggtgca ggagaatata agacgtttga accttcatta
    98341 tctgaaacgt ttatttttgt attagaaggg gaattagagt taagcttagg agagacggtc
    98401 tatgtggcaa aagcaggaca gtcgatctat tatcaagcga caaaacaaca tcaattaagg
    98461 aatcatctca aaaaacgaac aaaagtattg attgttgcaa cgcaatcata tttgtaatat
    98521 agggggtaaa aacgtgggga aaccaatcat ttcatttgat caagtagtga aacgctatga
    98581 tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa caaggaaaat tttatacact
    98641 gcttggtcct tccggctgtg gtaaaacaac gatcctacgt attatagctg gattcaccga
    98701 agctacagaa ggggacatct attttgaagg gaaacggttg aatgatttgc cagctaataa
    98761 gcgacaagta aatacagtct tccaagatta tgcacttttt cctcatatga atgtttttga
    98821 taacgttgct tttggattga agatcaagaa aatgtccaaa tcagatatcg aaaagaaggt
    98881 aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa aaacgggaga ttagtgaaat
    98941 gtctggtggt caaagacagc gtgtagcgat tgcacgagca attgtaaatg aaccgaaagt
    99001 attgctatta gatgaaccgt tatcggcgct tgatttgaaa ctacgtacag atatgcagta
    99061 tgaactgcgg gatctgcaac aacgattagg aatcacgttt atttttgtta ctcatgacca
    99121 agaagaagcg ttggcaatga gtgatgagat ttttgtcatg aataaaggga aaatcgtcca
    99181 aagtggtaca cccgtagata tttatgatga gccaatcaat catttcgttg ctgattttgt
    99241 tggggagagc aatatcgtgg acggtgtaat gatcgaagat aatctagttg agtttgttgg
    99301 taagcaattt gaatgtgtcg atggcgggat gcgtctaaat gaaccagttg aagttgtttt
    99361 acgcccagag gatttgacga tcacgacacc agataaagga aaactagtcg taacggtcga
    99421 tactcagctg tttcgtggtg tccattatga aatcatctgt tatgatgaac agcaaaatga
    99481 atggatggtc cattcaacga aaaaagctaa agaaggaagc aaagtaggat tggcctttga
    99541 gccagaagat atccatgtca tgcgtttcaa cgagtctgaa gaagaattcg atgcacgtct
    99601 tgacagctac gaagaatagg ggggacaaga atgaaaacaa tgcgcaggat ctattccgtc
    99661 ccttatattt tgtggctcgg tttattcgtg atcgcgcctg tattaatgat catttatcaa
    99721 tctttttttg atatgaatgg gcaattcacg ctgaataatt atctatctta ttttacgtcg
    99781 gggacatcgc tttctatgac gatcaattct gtatggtatg catttttgat cacgtttttc
    99841 acgctgatca tcagttaccc gactgcttat tttttaacaa aactgaagca caagcaatta
    99901 tggctgatgc tgattatttt gcctacatgg gtgaatctgc tgcttaaagc ttacgcattt
    99961 attgggattt tcagtgtgca cggcagcgtg aatcatttct ttgattttat cgggatcggg
   100021 ccgaaacaga tattattcac agacttcagt tttctgttcg tggcaacgta tatcgagatt
   100081 ccatttatga ttatgccaat tttcaatgca ttggaagaga tgaatccttc tttgatcagt
   100141 gctagtagag atttaggcgc gaatagcttt gagaccttcc gacgagtgat ttttccgtta
   100201 tcattaaatg gtgtgaaaag tggtgtccaa gcagtattta ttccttctct ttctttattc
   100261 atgctgactc gtctgattgg cggaaatcgg gtgatcactt taggaacggc tatcgaacag
   100321 catttcttgg ttacacaaaa ctgggggatg ggttctacaa tcggtgtgat tttaatcatt
   100381 gctatgttca tcgtgatgct cctgacaggc gagaagaaaa aaggaggtcg ctcataaatg
   100441 aaaaaattta aatggtcgta tctttattta gggatcgtct ttttattctt atatgcaccg
   100501 atcttctatc tgatttttta ttccttcaat gaaggaggaa cgatgaacag ctttacagga
   100561 tttacttggg aaaattatga agctgttttt gaagatacac gtttgatcat tatcgtattg
   100621 aatacgttca tgctggcttt cttatctgca ttgatcgcca cgatcatcgg gacatttggg
   100681 gctatgggaa tctattacac gaaaaaacga cgtgcacgga cagcgttgtt gagcatgaat
   100741 aacatcttat tagtctctcc tgatgttatt atcggtgcta gtttcttgat tttctttaca
   100801 ctactaggat tcggattagg ttttcagagt gtattgcttt cccatattgc tttcagcatt
   100861 ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa tgaacgattc aatggtcgat
   100921 gcggctagag accttggtgc taataatttc caagtagtca aaaatatcat tttgccttat
   100981 ctttctccag gaatcatagc tggatatttt atggcattta cctattctct tgatgatttt
   101041 gcggttacat ttttcgtcac aggaaatggc tttacgacgc tctcagtaga gatctattct
   101101 cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa gtgcgttagt tttcttgttc
   101161 tccatgatat tggtcattgg atactacttt attagtaaag aaaaccgtcc aaaacggctg
   101221 aagaaaaaca gaagagtacg cgagggggtg gcacggctgc gatgaaaaaa ttacaatctc
   101281 tgtttttagg tattctcatc attattgcgg tgctgctgct tggtgttcgt caacttgaaa
   101341 aaacaagtgg catgagtgga gcaaaggtac ttaccatcta taactgggga gattatatcg
   101401 atcctagttt gatcacgaaa tttgaaaaag aatatggata caaagtgaat tatgaaacat
   101461 ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg tgggacaaac tacgacatcg
   101521 ctataccaag tgaatatatg atccaaaaaa tgatcaaaga aaaactagta cttccattag
   101581 atcattcgaa gatcaaagga ttaaataata ttgatccacg atttttagac ttggattttg
   101641 atccagataa tcagtattcg attccatatt tttggggaac tttagggatc gtatataacg
   101701 ataaattctt tgatgcaaat caaattaagc attgggatga tttgtggaag ccggaattaa
   101761 aagacagttt gatgctgatt gacggtgcac gagaagttat ggggctttct ttgaatagtt
   101821 taggctattc tttgaatagt aagaatatgg cacagttgac agaagcagca aataaattga
   101881 atcatttaac accaaatgtc aaagcaatcg tggcagatga gatcaaaatg tatatgatca
   101941 atgaagaagc ctccgtggca gtgacatttt ctggcgaagc tgccgatatg atgtgggaaa
   102001 atgaacattt acattatgtg atcccaccag aaggatccaa tctttggttt gataatatcg
   102061 ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga tttcatcaac tttatgctag
   102121 aaccggaaaa tgcggcacaa aatgcagagt atattggtta ttctacacca aatgaagaag
   102181 caaaagcgtt attgccaaaa gacatttcgt cggacgaaca attctatcca tctgatgaca
   102241 cgatctccca tcttgaagtc tacaaagatc taggatcgaa atacctaggc atctataatg
   102301 acctattctt agaattcaag atgtatagga gataatggca aaactcagta agaatgggga
   102361 cttatcaacg ttctaaagag ctaaaaatcg tcctttttct atctgagttt tccaatcttc
   102421 taccaaaagc ggatgaaacg ccgctttttt tgtgttattt tattttttct accacgtttt
   102481 tctaccagag aaaagtggac aaaaccattg taaaataggg cccgagacgg gctagtaaat
   102541 ctaagattga aaaagttaca gaaggataaa gtaattggaa actatgcaaa tatacttaac
   102601 ccttctgtaa tctattaata aaactaatat atgaatccag ttcaatattt gtagctatga
   102661 aaaaacataa aaatcatatt agataaaaac taaattggta gataaattat tttgtcagat
   102721 atctgaggca aatattcgac tatttgtgtt tcaaagtaaa aattggaata caaatataga
   102781 atagacatat tgcagccgat agtttattat gcttttagac acattaaact ggaggaaaaa
   102841 atgaaaaaga gcgctaaaga tcgagttttt gatgagctga tacgactaac agggaatatg
   102901 tcttctgtca ctgccgagaa aatttcgaag aatctaggta tctctcgtca gaatgccagc
   102961 cattatttga cgagattagt ggaagataaa aaggtcgaga aattaccggg aaagcctgtt
   103021 ttgtggaagc ctttagatga atatgcggtt gtagataata cggagcaaat aaacgaagcc
   103081 tttcattctg tagttggtca tgacggaagt ctgagagagg tcattcaaaa atgtatagcc
   103141 gctgtcaaat atccgcctaa cggactcagt gtattaataa atggagccac gggtgttgga
   103201 aaaagttttt tagccacaaa aatttttgaa tacgctatcc atgagcaaat tattgaaaaa
   103261 gatgcaccat ttgctatctt aaactgtgcc gattatgcgg acaatcccga actgctttcg
   103321 gcaacactgt ttggatacaa gaagggggcc tttacggggg ctgaaaagga taccgatggt
   103381 ttattggcaa cggccaacaa cggctatctt tttctcgatg aggtccatcg cctatcgaaa
   103441 gaaaatcaag aaaagctctt tctatttatt gatactggaa actatcgtcc agtcggtgaa
   103501 aatgtaaatt ggcattctgc taaggttcgt tttattttcg ccacgacgga aaaaggtgaa
   103561 aattatttat tagacacctt tgatcggcgt attcaaatta gtgttatgtt gccaactttt
   103621 gaagatcgcc ccattcgaga acgattagag ctgattcaat tattttttca aaatgaagcg
   103681 gatgttttac aaaaagatat tattgtttca agagaagcct tgtctttaat gttatctcat
   103741 ccctttagtg ggaatattgg taagctgaag aatatcatta aaatcagttg tgcggatgtg
   103801 tatagtcgaa cacaagaaga accattaacg attgggaaaa atgagatctt gattcagtta
   103861 aatatgctgg aatccgaagt agaaagcttg ccgattgcta gtttattgat aaagaaaaat
   103921 caaaagataa aaacggatcg aatcaaaccg tttgattatg attcgatttt ggaaaaaatt
   103981 actcagctga taaatgaagg taatttcaat gaaaattttt cttcgataaa gaatgaagta
   104041 cagcatttgg tgcatcacat tgataaggat tcttttttat caatgagtag aaaattgatg
   104101 attcagatgt ttagaaaggt ctgggtatcc gttttaggaa aaacgtacgg attgacgacg
   104161 agtggacctg ttgcagaaat tattagcaga tcctatgtca tttccccaag ctataagaca
   104221 gacttaaccg ggataaatcg tacattagct gcaaatctac ctcggacgac ttacttatgt
   104281 aatcagttct tggagcattt accaccgctc tcagaaaagg acgagaactt tctaagactg
   104341 cttattgtcg tgactttatc tgattatgta gatgaatcca tcgagctgaa gggcctgttg
   104401 cttgctcacg gagatagtac ggctagcagc attcaagcag tagtgaatca attatgcggc
   104461 aattatgttt ttgaagcgtt ggatatgcca gtagagagta atgtgtcaga gattgtcgaa
   104521 tcgacaagga atttcgtgaa acggcaagtg aatacagata gtctagtgct gatcgtagat
   104581 atgggctctt taaatcaaat ttacacacag atcaagaacg atttaaaagg ggagttactt
   104641 cttctgaata atttgacgac ttccattgca cttgatattg gcttgaagat gacgatgaat
   104701 gcgccattta aacagattgc ggaggatgca gcagacaagt attcaatcaa tgttcaatac
   104761 tttgaaggct tttctcaaag tagcaacatt atcatttcct gtatgtcggg tttggggatt
   104821 tcggacaaat tgaaagatgt ttttcgtcat catttatcgg atgaatccat cgaagttttc
   104881 acgaaggact acagagaact acgtgaatta atcgaccaaa atgatgaaac ttattttgac
   104941 aagacaaaat tggtcatcac gacttcggat cttccacgtt ccttttcaat tcctaatatt
   105001 aatgtttatg acatcttaga cggtgatgga gcagccaatt tggagcatat tttgagtcct
   105061 gaaatgaagc cggcagaatt taaccgcttg attcaggagc tagtaaaatt cttttcgttg
   105121 gaaggaattg cggaccgttt gaactttctg aacccaacga tcgtgatgag tgaagtggaa
   105181 acagtcattt ctaaatatga aaattactat catttcactt taaatggccg tgtaaaacta
   105241 aatctctata tgcatacggc attgatgatg gaacgtttat tcttggctcg gacagaaaaa
   105301 tctgaagaac cgaattatga tttgaaacca gatgaagatg aattttatta tgtttctaga
   105361 agtatttttc aatcaatgga gatgaaatat aattttaaag tcaatgctta tgagctttcg
   105421 ctgctctatg aattattagg accgtatatt caaaaataaa gtgtctaaaa aagtgtctaa
   105481 tctgtttttt ttaggttaga cacttttttt aatgtgaatt aacgtgatta aaccaatgtt
   105541 tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac agaaagaaag gaattaacca
   105601 tatgaagaaa attctaatag cgactcacgg tcacttagct tcaggctttc ttagcagtat
   105661 tcaattactg acggggaagg ttcaagagat tacggtgatt aatgcttatg tggatgagtg
   105721 tgattttgaa caggagttga atgtatttgt aaaccaggtg aacgagcagg atcagatttt
   105781 tgtttttacc gatttatttg gcggaagtgt caatcagaag attaccaaaa cctttttgga
   105841 gaaagagatt gctgtaacta ttttgtctgg ctttaatctg ccaattctgc tcgaaatagt
   105901 attggcaacc aaggaattaa caaaggagga tattcaggaa ctggtaaata aatgccaaca
   105961 ggaattaaag atgaccattc ttgatgaggt acatgtagta aatgaagagg aagatttttt
   106021 ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg aacgattaat tcatggacaa
   106081 gtagcgatga cttggacaaa gagtctaaat ttaaccgggt tagtagtagc aagcgatgag
   106141 gcggctacca acgatattca aaagatgaca ttgaagatgg ctgcaccatc gaatgtcaaa
   106201 gtcattatta aaacagttga tgacgcgatt catttgttaa aagatccgcg ggcgaaggac
   106261 atgcgcttga tggttttagt ccctaatgtc aaagatgcgg taaaagttgc gaaagctttc
   106321 ccaacggaaa tcgaattaat gaacgtgggg aatgctggga aaatgacacc gccatcggaa
   106381 gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt cagaaattca agacttaaaa
   106441 gaattagttg aactttatcc agataccgtt tttcagccta caccggcgat ggaaaaacgt
   106501 tcagctatct ctattttgag tgatttttag aaaaaggaag gagaaaaatt atgtatgatg
   106561 catttgtagt cgctttggcc gtttttattg gaattgctgg acatgaaatg ttcgggatgt
   106621 ccatgctgag tcgtccgatt gttgttgctc cgttagcagg agcgttgatg ggggatttgc
   106681 agacggggtt gattgtcggg gcgtcactag aagccatctt tatgggtgtc gtcaatatcg
   106741 gaatctctag tacggcggag ccttctttgg ctgctgggct ggctacagcg ttcgcaatca
   106801 aaatgggagg aaacgtggat gcgattattc ccattgtctt tccactagca gtattaggat
   106861 tacagttaat gaatatgatt ttttcttttg tatgtggacc gatggctgct aaatttttca
   106921 aatttgcaaa atctggtgat caaaaaggaa tcatcaatct gcacttcttt atgtggtttg
   106981 tccactatgc gctttatgct cttattccat tctttgccgt tttatttgga gcagatgttg
   107041 tcaaaagcgt cttggataat attccagagg tcatcatgaa cggattaaca gtcgctggaa
   107101 atctgctgcc ggcagttggg atggcgatgc tgttacggat gctttggagt aaaaatattg
   107161 ctgtgtggtt tttctttggg gccgttgtta tggcgtatct cgagttacca ttgattgcaa
   107221 ttgccgcagt tggaacgatt attgcaattg ttatagccca aagagatcta caactaaaaa
   107281 atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa tgcaacgctt gaccaagaag
   107341 aggaggattt ctttcaatga aattagctga tggaatttct aaagatgaga aaaaattggt
   107401 tcggaagatg ttttggcgct cgatgactat gtatgcttcg gtgaatccgg tgacgatggg
   107461 cggcggtggt ttcgcctatt ctatgatgcc cttcattaat catttctaca aaagtgaaga
   107521 agatcgagct gcggcgctgg aacgtcatac acagtatttt tcgacgacca ttccttgcgc
   107581 gtcctttgtc atggggattg cggcatcgat ggagaaggaa aattctgaaa aagctggttt
   107641 tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg ggaccattgg ctggaattgg
   107701 cgattcattg ttctggggag tttggcgtgt cgttaccgcg ggactagcga tcaacttagc
   107761 caatgccgga aatattttgg caccaattat ctttttagtt atgttcaata tacctaatta
   107821 cttgatgcga tactatggcg gcgttttagg ctattcatta ggctctaaat atattgaaaa
   107881 gctgtatgcg aacgggttaa ttgaaattct gacaaaagct gctagtatct tgggtctgat
   107941 catggtaggt gcaatgacga tcagtatggt caaattcaat accgttttga gcttttcaat
   108001 gaagggcgaa gaactaatga atgtccaaga aatcctcgat agtattttta aaggaattgt
   108061 accgttattg gtaacattga gctgtttcaa attactagat aaaaaagtcg gcgtcatcaa
   108121 aattattttt ggaatggtcg tcttgggaat tttattgtca ttacttggaa tcgcatagta
   108181 ggaagctgca gctactaggc tgcagttttt ttagcaagga gggaaaagat gactattaat
   108241 gaagtaattt attcacccaa acacccattt tttaatgctg agttaaacga cggaaatgtg
   108301 actacttcaa acgaatacag ctactatcgg aatattcctg atgtaaaggg attgaccttc
   108361 gatgtaagta atcaaaagac attagcaaac cttgatgttt gcgggacgat tcaaacgttg
   108421 accttttatc gagaaaattt actaactgag gaaaaaccag gtgtttgggt caataaacag
   108481 ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa acgggattgt ctaccatttg
   108541 tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag actgtttgcc tagatatatt
   108601 catcattatt cgacgtttaa ggtcattgta gtatcctttg ctcccatact ggctaataag
   108661 cgtttaagta tgcttgttca acaggtttac attgtgaatg aaacacacga aagccttcaa
   108721 gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt cggatcagca aaatgtgttg
   108781 atttctcaaa aaggaaataa ggacacgatc gcacaacagg aagtggcaag cttcgccatt
   108841 gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt ttcaggaatc tgatatcgaa
   108901 aaatggctga aggaaacatt gaactatttt gctcagctat ttgggcagct gtcaatgcca
   108961 aacgatagag ttgtccatct ctatcgtcgt gcactttatc aaagcttttc tagtttcggt
   109021 atgaatcgac agggacaggt cgtcggcagc aattggggca gctatcctgc tactaatcga
   109081 atttggaata aggatatgta ttacgcatca ttgccattca ccatgtttga accggagtta
   109141 tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta agtttcctgg ttcaaaattt
   109201 acaggaggta tcaatcattc tctgagtaat tcgttatcca gtgcgttact ggctagtctt
   109261 tattttgaac atactgggga tacaaaattc tttgagcggt gtccgcaggt tctactgaat
   109321 gcttcaagaa ttattgagga catattagct caacgtcggt caggggaacc aatgcttttt
   109381 ccatctgttt ggttatcaga cgcctttgca cttggaaagt atcataccgg cagcaatctt
   109441 tgtctttgga aggcctgtat tggacttgca gaaatttttg aagccctaga acaactgagt
   109501 cttagcaagc gatacaaaaa cattgcctgt aaactaaagg aatcaatttg taaacaaatg
   109561 actatcgatg gtccttttgg tgagcaattt ttggagggaa tcggtgatga ggagaaaaca
   109621 acctattcag tgaagcacta tcaaaaaccg attcttgagc aagggttaat ctttttaagt
   109681 gatgtgattg tggatggaaa aattaattta ttgatgcatg atggagaaga atcagacact
   109741 acgttgatgc ctttttatca atttttagat aataaagatc atttatatca gaacaccatg
   109801 gccttttctg ctagttcttt caatccaacc tatagcgagg aaatcaaagg catcacttgg
   109861 ggattagaat ccggcgccac ttttcctggc tttatcactg ttttaatgag cgagctgcac
   109921 aaccctcaag ctttcgtaac ccgattagaa gaactaattt gtttggcaga tttagacggt
   109981 tcgtggtggt ggtggccgta tcgcttagag gctcaacaag gagaagtcgt gcgagacttt
   110041 ggctgcggca aatgcggctg ggcatcgggt ctttttgtca gcctaatgat cagtcaatat
   110101 ttcggattga aattcaaaaa aggagtattg caaattgatc ctgttgataa tttaacgttt
   110161 tcttggaaga atttgaagtt cggtcaagcc ttcatttcga ttgaatgtac gcaatccgag
   110221 ttgagtattt caaatttgtc agaaaagcca ttgaaaataa gtgatacgaa aaaaattctt
   110281 cacgtagaaa agggaaaaac gattcacatt caaaggagaa aaagatgagt atactaaaaa
   110341 atattaccca atttaaaaaa atccagagtc gagcaatcac tcccgaaaat ccaacaggcg
   110401 agaaaggtaa agctgcgatg ttgccaagcg agctaggacc aagccgcaaa ggtcgtggcg
   110461 caattccgct gacttctggt gaagaaactg tgattgcgga tattactgga acgggaataa
   110521 ttcgtcatat gtggatgacg attcgtgatc gaacagaatt tggcagtttt gttttacgtg
   110581 atttaatctt aagaatttat tgggatgggt cggatagccc tgctgttgaa agtcctttag
   110641 gggatttctt ttgtaatggg tttggagagc gttgcgaggt gaactcgttg ccgatagtgg
   110701 ttaatcctac agggggcttt aattcctatt tcgaaatgcc tttccaaaca tccgctaaaa
   110761 ttacgatcga gaatttacat ccgaaggata tgtcctcatt tttttatacg ataaattacg
   110821 ctttggtgga cgaattggaa gaaaatagcc tatattttca cgcaaattgg catcgggaac
   110881 gcattaccaa attacaggag gattataagt taattgatcg attaaagggt caaggctatt
   110941 acgtgggtac ttaccttgcg cttacctgcc tagaacgtta ttggtggggc gaaggcgagt
   111001 ttaaattcta cattgatggg gatagtgagt atccgaccgt aacctcaaca ggttcagaag
   111061 attattttgg cggggcgtgg gcctttcatg agtacgatga gttcggacgt ccacatgcga
   111121 aaaactattc aacagccttt ttagggtatc cttaccaatc aaatagagat gccacaagag
   111181 atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt tggtgacgac gcaatgccac
   111241 gacatggtct ttatcgttgg catcttcaag accccatctg ttttaccgaa gatttatcgg
   111301 tcactttaca acaaatcggc aacgatgatg ttcgcttatt tgagcggcaa gacgatgttg
   111361 ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa tactaaacgt catttcactg
   111421 atcgagaacg cttgccgcgt taatgaagtg tttttcacat attcgaaggg aggaacctat
   111481 gatgatatat ggggcaattg aagctggagg gactaaattt gtctgtgcag tcggtactga
   111541 ggaattaacg attttggaaa gagtcagttt tcccacaaca actccagttg aaacaatgaa
   111601 gcaggtcatt gattttttta aaccttttaa ggaacaattg gcaggcatcg gggtcggctg
   111661 ctttggtcca attgatattc aatgcaaatc aacgagtttc ggtcatatca catcaacacc
   111721 aaaacttgct tggcaaaatt tcaattttgt aggaacgcta aagcaatatt tcaatattcc
   111781 gattgaatgg acaacagatg tcaatgcggc ttgctatggt gaatatgttg ctggttctgg
   111841 caaagggtta gcaggtgttg catacttcac tgtgggaaca ggtatcggag gtggtgcctt
   111901 gttcaatggg actttcgtgg aagggttcag tcatccggaa atggggcata tgttgatcaa
   111961 gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc caccaagatt gtttagaggg
   112021 gatggcagca ggaccagcga ttgaaaaacg gttgggtgta aaaggccaga atttattggc
   112081 tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc caatgtgcat acaatacaac
   112141 actgatgttt tcgccagata gaattatttt tggcggtggt gtaatgaaac aagaacacat
   112201 gaagaaaaaa gttcaggata aatttgttga gcttataaat ggatatgttg aaataccacc
   112261 aattgatagc tacattatta cgcccgaatt aggtgataat gccggaataa tcggcggatt
   112321 agcattggcc agaaaagcgg taagaaataa gcaaccataa ttaatgaatg tttcattgat
   112381 actcatgtca aaagtattta accgagtaat caaatgatca aatgaagttg gataaagcga
   112441 aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt gttttcatta aatcactgaa
   112501 aggaaatgct tctgcgtttc cttttttctt tttgttgtgc gctgtatacc ttgaggatat
   112561 tcactggtgg cgtacactta aatagtaata tattgtgtta ctttatgtct gtggtattag
   112621 ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt tgacttcata atccttggct
   112681 taatgtttga atttttttgg tgatagattt tttcagccgt ataaaataaa tcgtaattct
   112741 attatagaga gttgtgatta taaaaagata gcaagaaaaa aaaagaaagt ttctatacat
   112801 tttaaatatt ttattcctat ttctattgct aaaacagtag aaattctata tttaccttta
   112861 actctcgctt ctcttgcaaa tgtagttagc attttaatcg ataataaact caggtagtta
   112921 tattaccttg aaatgattga taattttgtc acttctattt tttttgcaat aaatttttta
   112981 ttttagtgaa aaataagaaa ctatttcttt gaaggcgata agttagaaaa atattattgt
   113041 agtct
//
LOCUS       DAJSBM010000003       106207 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJSBM010000003 DAJSBM010000000
VERSION     DAJSBM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.003"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            211..603
                     /locus_tag="ONT67_000230"
     CDS             211..603
                     /locus_tag="ONT67_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2353999.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="ONT67_000231"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="ONT67_000231"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCR2354000.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="ONT67_000232"
     CDS             3164..4774
                     /locus_tag="ONT67_000232"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCR2354001.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="ONT67_000233"
     CDS             5066..5935
                     /locus_tag="ONT67_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCR2354002.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ONT67_000234"
     CDS             6100..7371
                     /locus_tag="ONT67_000234"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2354003.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ONT67_000235"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ONT67_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR2354004.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ONT67_000236"
     CDS             8762..9025
                     /locus_tag="ONT67_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR2354005.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ONT67_000237"
     CDS             9470..9919
                     /locus_tag="ONT67_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2354006.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ONT67_000238"
     CDS             9976..11394
                     /locus_tag="ONT67_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2354007.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ONT67_000239"
     CDS             11394..12254
                     /locus_tag="ONT67_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR2354008.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ONT67_000240"
     CDS             12428..13201
                     /locus_tag="ONT67_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2354009.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ONT67_000241"
     CDS             13348..13713
                     /locus_tag="ONT67_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCR2354010.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ONT67_000242"
     CDS             complement(13861..15069)
                     /locus_tag="ONT67_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2354011.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ONT67_000243"
     CDS             complement(15088..15366)
                     /locus_tag="ONT67_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354012.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="ONT67_000244"
     CDS             15520..16215
                     /locus_tag="ONT67_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2354013.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="ONT67_000245"
     CDS             complement(16657..17454)
                     /locus_tag="ONT67_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCR2354014.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ONT67_000246"
     CDS             complement(17451..18635)
                     /locus_tag="ONT67_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2354015.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ONT67_000247"
     CDS             complement(18660..19907)
                     /locus_tag="ONT67_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCR2354016.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT67_000248"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ONT67_000248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR2354017.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ONT67_000249"
     CDS             21887..22201
                     /locus_tag="ONT67_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR2354018.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ONT67_000250"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ONT67_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR2354019.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="ONT67_000251"
     CDS             22886..23530
                     /locus_tag="ONT67_000251"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR2354020.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ONT67_000252"
     CDS             23545..23874
                     /locus_tag="ONT67_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCR2354021.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ONT67_000253"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ONT67_000253"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR2354022.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ONT67_000254"
     CDS             24862..25686
                     /locus_tag="ONT67_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCR2354023.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ONT67_000255"
     CDS             25679..26026
                     /locus_tag="ONT67_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCR2354024.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT67_000256"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ONT67_000256"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR2354025.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ONT67_000257"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ONT67_000257"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR2354026.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT67_000258"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ONT67_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCR2354027.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT67_000259"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ONT67_000259"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR2354028.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKNQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31656..32450
                     /locus_tag="ONT67_000260"
     CDS             31656..32450
                     /locus_tag="ONT67_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354029.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ONT67_000261"
     CDS             32440..34482
                     /locus_tag="ONT67_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354030.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="ONT67_000262"
     CDS             34763..34912
                     /locus_tag="ONT67_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCR2354031.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="ONT67_000263"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="ONT67_000263"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCR2354032.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="ONT67_000264"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="ONT67_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCR2354033.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="ONT67_000265"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="ONT67_000265"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCR2354034.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="ONT67_000266"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="ONT67_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCR2354035.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="ONT67_000267"
     CDS             39329..40411
                     /locus_tag="ONT67_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCR2354036.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40411..41214
                     /locus_tag="ONT67_000268"
     CDS             40411..41214
                     /locus_tag="ONT67_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2354037.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41284..42024
                     /locus_tag="ONT67_000269"
     CDS             41284..42024
                     /locus_tag="ONT67_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2354038.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42035..43039
                     /locus_tag="ONT67_000270"
     CDS             42035..43039
                     /locus_tag="ONT67_000270"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCR2354039.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43084..44928)
                     /locus_tag="ONT67_000271"
     CDS             complement(43084..44928)
                     /locus_tag="ONT67_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354040.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="ONT67_000272"
     CDS             45140..45658
                     /locus_tag="ONT67_000272"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2354041.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="ONT67_000273"
     CDS             45786..46469
                     /locus_tag="ONT67_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCR2354042.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="ONT67_000274"
     tRNA            46778..46851
                     /locus_tag="ONT67_000274"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="ONT67_000275"
     CDS             47010..47369
                     /locus_tag="ONT67_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCR2354043.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="ONT67_000276"
     CDS             47492..49123
                     /locus_tag="ONT67_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCR2354044.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="ONT67_000277"
     CDS             complement(49185..49826)
                     /locus_tag="ONT67_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR2354045.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="ONT67_000278"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="ONT67_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR2354046.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="ONT67_000279"
     CDS             50443..50787
                     /locus_tag="ONT67_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCR2354047.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="ONT67_000280"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="ONT67_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR2354048.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="ONT67_000281"
     CDS             51527..54334
                     /locus_tag="ONT67_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2354049.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="ONT67_000282"
     CDS             54525..55016
                     /locus_tag="ONT67_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2354050.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="ONT67_000283"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="ONT67_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR2354051.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="ONT67_000284"
     CDS             56141..56944
                     /locus_tag="ONT67_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR2354052.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="ONT67_000285"
     CDS             56965..57879
                     /locus_tag="ONT67_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR2354053.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="ONT67_000286"
     CDS             57962..58336
                     /locus_tag="ONT67_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCR2354054.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="ONT67_000287"
     CDS             58475..58720
                     /locus_tag="ONT67_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2354055.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="ONT67_000288"
     CDS             58892..59956
                     /locus_tag="ONT67_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2354056.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="ONT67_000289"
     CDS             60202..60627
                     /locus_tag="ONT67_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2354057.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="ONT67_000290"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="ONT67_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR2354058.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="ONT67_000291"
     CDS             61166..62020
                     /locus_tag="ONT67_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCR2354059.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="ONT67_000292"
     CDS             62021..63358
                     /locus_tag="ONT67_000292"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR2354060.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="ONT67_000293"
     CDS             63363..63587
                     /locus_tag="ONT67_000293"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR2354061.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="ONT67_000294"
     CDS             63589..64479
                     /locus_tag="ONT67_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2354062.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="ONT67_000295"
     CDS             64488..65306
                     /locus_tag="ONT67_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCR2354063.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="ONT67_000296"
     CDS             65413..65862
                     /locus_tag="ONT67_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2354064.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="ONT67_000297"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="ONT67_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR2354065.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="ONT67_000298"
     CDS             complement(67617..67733)
                     /locus_tag="ONT67_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCR2354066.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="ONT67_000299"
     CDS             complement(67806..68762)
                     /locus_tag="ONT67_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2354067.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="ONT67_000300"
     CDS             68933..69301
                     /locus_tag="ONT67_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCR2354068.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69418..69560
                     /locus_tag="ONT67_000301"
                     /pseudo
     CDS             69418..69560
                     /locus_tag="ONT67_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719784.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
     gene            69584..70015
                     /gene="mraZ"
                     /locus_tag="ONT67_000302"
     CDS             69584..70015
                     /gene="mraZ"
                     /locus_tag="ONT67_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR2354069.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            70036..70995
                     /gene="rsmH"
                     /locus_tag="ONT67_000303"
     CDS             70036..70995
                     /gene="rsmH"
                     /locus_tag="ONT67_000303"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR2354070.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            71000..71401
                     /gene="ftsL"
                     /locus_tag="ONT67_000304"
     CDS             71000..71401
                     /gene="ftsL"
                     /locus_tag="ONT67_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR2354071.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71402..73594
                     /locus_tag="ONT67_000305"
     CDS             71402..73594
                     /locus_tag="ONT67_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2354072.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73620..74582
                     /locus_tag="ONT67_000306"
     CDS             73620..74582
                     /locus_tag="ONT67_000306"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR2354073.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74596..75957
                     /locus_tag="ONT67_000307"
     CDS             74596..75957
                     /locus_tag="ONT67_000307"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR2354074.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75983..77071
                     /gene="murG"
                     /locus_tag="ONT67_000308"
     CDS             75983..77071
                     /gene="murG"
                     /locus_tag="ONT67_000308"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR2354075.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            77086..78306
                     /locus_tag="ONT67_000309"
     CDS             77086..78306
                     /locus_tag="ONT67_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCR2354076.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78462..79787
                     /gene="ftsA"
                     /locus_tag="ONT67_000310"
     CDS             78462..79787
                     /gene="ftsA"
                     /locus_tag="ONT67_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR2354077.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79810..81051
                     /gene="ftsZ"
                     /locus_tag="ONT67_000311"
     CDS             79810..81051
                     /gene="ftsZ"
                     /locus_tag="ONT67_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR2354078.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            81068..81745
                     /locus_tag="ONT67_000312"
     CDS             81068..81745
                     /locus_tag="ONT67_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCR2354079.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81759..82358
                     /locus_tag="ONT67_000313"
     CDS             81759..82358
                     /locus_tag="ONT67_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCR2354080.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82373..82660
                     /locus_tag="ONT67_000314"
     CDS             82373..82660
                     /locus_tag="ONT67_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCR2354081.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82709..83491
                     /locus_tag="ONT67_000315"
     CDS             82709..83491
                     /locus_tag="ONT67_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR2354082.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83512..84219
                     /locus_tag="ONT67_000316"
     CDS             83512..84219
                     /locus_tag="ONT67_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCR2354083.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84481..87279
                     /gene="ileS"
                     /locus_tag="ONT67_000317"
     CDS             84481..87279
                     /gene="ileS"
                     /locus_tag="ONT67_000317"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR2354084.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87328..88854)
                     /locus_tag="ONT67_000318"
     CDS             complement(87328..88854)
                     /locus_tag="ONT67_000318"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR2354085.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88869..89516)
                     /locus_tag="ONT67_000319"
     CDS             complement(88869..89516)
                     /locus_tag="ONT67_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCR2354086.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89700..90029)
                     /locus_tag="ONT67_000320"
     CDS             complement(89700..90029)
                     /locus_tag="ONT67_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCR2354087.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90206..90934
                     /gene="sfsA"
                     /locus_tag="ONT67_000321"
     CDS             90206..90934
                     /gene="sfsA"
                     /locus_tag="ONT67_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR2354088.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90950..91963
                     /locus_tag="ONT67_000322"
     CDS             90950..91963
                     /locus_tag="ONT67_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2354089.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91963..93240
                     /locus_tag="ONT67_000323"
     CDS             91963..93240
                     /locus_tag="ONT67_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2354090.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93303..96005
                     /locus_tag="ONT67_000324"
     CDS             93303..96005
                     /locus_tag="ONT67_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2354091.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96157..96474
                     /locus_tag="ONT67_000325"
     CDS             96157..96474
                     /locus_tag="ONT67_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2354092.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96504..96824
                     /locus_tag="ONT67_000326"
     CDS             96504..96824
                     /locus_tag="ONT67_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2354093.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96932..98392
                     /locus_tag="ONT67_000327"
     CDS             96932..98392
                     /locus_tag="ONT67_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2354094.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98460..98681
                     /locus_tag="ONT67_000328"
     CDS             98460..98681
                     /locus_tag="ONT67_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354095.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98712..98894
                     /locus_tag="ONT67_000329"
     CDS             98712..98894
                     /locus_tag="ONT67_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCR2354096.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98894..99307
                     /locus_tag="ONT67_000330"
     CDS             98894..99307
                     /locus_tag="ONT67_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCR2354097.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99430..100611
                     /locus_tag="ONT67_000331"
     CDS             99430..100611
                     /locus_tag="ONT67_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2354098.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100682..100846)
                     /locus_tag="ONT67_000332"
     CDS             complement(100682..100846)
                     /locus_tag="ONT67_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354099.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            101000..101073
                     /locus_tag="ONT67_000333"
     tRNA            101000..101073
                     /locus_tag="ONT67_000333"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:101034..101036,aa:Arg,seq:cct)
     gene            complement(101110..101310)
                     /locus_tag="ONT67_000334"
     CDS             complement(101110..101310)
                     /locus_tag="ONT67_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2354100.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101615..102847)
                     /locus_tag="ONT67_000335"
     CDS             complement(101615..102847)
                     /locus_tag="ONT67_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR2354101.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            103101..103670
                     /locus_tag="ONT67_000336"
     CDS             103101..103670
                     /locus_tag="ONT67_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2354102.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103848..104288)
                     /locus_tag="ONT67_000337"
     CDS             complement(103848..104288)
                     /locus_tag="ONT67_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR2354103.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104446..105210
                     /gene="map"
                     /locus_tag="ONT67_000338"
     CDS             104446..105210
                     /gene="map"
                     /locus_tag="ONT67_000338"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR2354104.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105242..106165
                     /locus_tag="ONT67_000339"
     CDS             105242..106165
                     /locus_tag="ONT67_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCR2354105.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT        34591 a        18239 c        22460 g        30917 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aactttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aattttttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggcatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagaacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgatt tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaatacctat ttctgaatat
    40441 cggaaaaaag cacgagtttc tttggaaaat caatggggaa tcaatacttg gcttgttttc
    40501 ttatctataa tatttacgag cgtcatccag aacttttttg acggtttcag tccttttccg
    40561 gaagaaagca tgcagcagca cttattgagt tttctagtcg ataatttact tttattcgca
    40621 ttcaactatg cattgtttta cattgcgcta gtagttgtac ggggtggcag accaaaaagt
    40681 aaccttttat tttcagtttt ccaaagaaag tattttggtt cgatacttgg catcaatttg
    40741 atcaattcag tcatcaactg gctactcaac agtttaatct ttctgcccat gtttttaatc
    40801 agcggagtga gtacttatat gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc
    40861 atgccagaag attttcttga tatcagcttt gttttatcga tgatagggat gatttttgtt
    40921 tcagcactca tcatgagtat tgttggcggt ttattccaat ttgctgcatg gacaaagttc
    40981 gactatcctg atttgtcggt tattcaatgt ttgaaatatg gctggttttt attgaaagat
    41041 agaattggta cgtatatttt gttgcagctg tcttttatcg gctggtattt gctaggagca
    41101 atcgctttgt tcatcggtct tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa
    41161 ttttatgaac aagcacggat tgaaaaagga ggtccagacg cctattttac ttagcatgta
    41221 agcagtttat tcatcaaaaa aataactttt gtggtaggat aaatctttga gggggaatgt
    41281 acgatgaaat taactgtttt aggatgctta ggagcgtatc cttataaaaa tcaagggaca
    41341 acaggttatt tgcttcaatc tgacgggttc aatttgttga tcgatgcagg tagtgctacg
    41401 ctgatcaaat tacaagaaca ccttgatcca cttgatctag atgctgtgat ccttagccat
    41461 tatcatcatg atcatattgc tgaccttggt gtattacagt attactggca acttcatcct
    41521 aagagaaatc cggagacatt gctttcaatt tatggacaca caaaagattc cattcacttt
    41581 gaagaattga cgttggaaaa tgtctcaaaa ggacatcctt attttgaagc tgaagaatta
    41641 aaaattggtc cttttctgat tacttttatg gaaacaatcc atccagtagt atgttacgcc
    41701 atgcgtatcg tagaagaaac aacggggaaa gtattcgtat ttacaggtga ttcaggttat
    41761 ttggaaagtt tcgctgattt tgcgaaagat gctgatttgt ttctagcaga tacctatctt
    41821 tttgaaggaa atgaacatca tcatgcccat ttcacttcaa aagaagctgg agaacttgcg
    41881 aaatcagcta acgtaaagaa acttgtactt acacacctac ctcagtttgg tgatttagaa
    41941 caattgaaaa atgaagcgca aaaggcagca ggcaaaggga ttgatataga acttgctgca
    42001 gttgacaaag tgtttaacat ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca
    42061 cgacccaaga atcaatttag caattgaaca atatttgctg caagaaatgc ctttagatga
    42121 acctattttg ctcttttata tcaatgaacc ttcaatcatc atcggcagaa accaaaatac
    42181 gcttgaagaa atcaatcgag attatgtgga agaaaatggc attcatgttg tccgtcgctt
    42241 aagtggaggc ggagctgtct accatgattt tggaaacctg aactttagtt ttatcatgcc
    42301 tgatgatggg gattcattcc gtgactttgc gaaagtaaca cagccaatca tccaagcact
    42361 tcatgaattg ggtgttgctg gggcggaatt gaaaggccga aatgatctag tcattgatgg
    42421 catgaaattt tctggcaacg cgatgtatgc taccaacggt cggatgtttg ctcatggaac
    42481 aattatgttc gactctgata tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa
    42541 aatcgaatca aaaggaatca aatcgatccg ttctcgtgta acgaatatca aaccatttct
    42601 tccagaagaa aagcaaaaaa tgacaacaga agaatttcgt caagaaatct tgttgaaaat
    42661 tttcggcgta gagtcagtag aagaagtaaa aacgtacgaa ttgactgatg aagattggaa
    42721 aaaaatcaat cgaatctctg aggaatatta tcgaaattgg gattggaatt acgggaagtc
    42781 tccagatttc aatttctctc gccaaaaacg tttttctatc ggttcgattg aagtccattt
    42841 gaatgtctca gaaggaatga tcaaagaagc aaaaatcttt ggcgatttct tcggactagg
    42901 agatatctct gacgtggaaa aaaaattggt tggacagaaa tatgataaag catctctatc
    42961 aaaaatagtc agccaaattg atgtgaagaa atattttggt gcgatagaac cagaagaatt
    43021 gcttacctta ctttattgaa aataaaaaat agcggaatcc acatatatgg attccgctat
    43081 tttttataca ggattcgggt aatttcgaga gagttgaaca gtgatccgat caatgaaagt
    43141 atctttttcc tgcgttagtg ttcctgtgta acgatccgct acgtctttca tcatcattaa
    43201 gtcaaaacgt tcctgatcat ctaattcttc tgtgatgcct cgactaaata ggtttgtatc
    43261 gtaatgtgca ctatgttcgc aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa
    43321 ataaattttt cgatacagtg gattttcgat ggcatgagtt tctctaaagg aatgctctaa
    43381 agcgtggatc aaaagttgag ccagatcgtc atcgttgata ggaagttctt cgggaagatc
    43441 tgcttggaag gttatctgaa tattccgttt ggcagctaac ttctggtaac gcgctaagat
    43501 aagatttgtc acctggtggt tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc
    43561 taatatcttt tccacatagt ttttagcttt tgtttggtcc gtatgcatta aatcttgcag
    43621 attctccatc tcttggacaa attccaggcg aagtgtattt acttcttgga tatgctgtcg
    43681 aagctgctca taatgttgct ccaacaaatc atttttgatt tttaagaaat ctacttgtct
    43741 ccgatctttg tcggtattta agatgtgatc gattagattg tagctgataa taacgattag
    43801 aatgataata tataaaatcg tagaccaatt gatcgttgtt tgatagatac ctaatgccgt
    43861 ttggatgtaa atcaagcaat ggataatggc taccaagacg atccaaggtg tacaagctac
    43921 ataaaaacgg ttattcttat atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct
    43981 tataacacta gcaatattag caatcggcat taattcagga agttctgcag gagtaaaagt
    44041 tgcataaagc agaacgccga taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca
    44101 ttttttatag taaaacattc ggctccaata gtagcagatg atgaaagctg acgttaagat
    44161 aataaataaa tgagataaaa aagaatgaac gaatggttca aaaagaatac cacttaaaat
    44221 atcttctgat acagcttcaa atcctaatgc caaagcaaag caacttaaga gaataagtga
    44281 gtattcaaga cgtttttgtt tatacatcaa gtagatcgta tagaaaaagg tccctatagc
    44341 gataaaaaca gcgattatca tcgtcaatac ttgcggcact gattgtgaat aaataaaact
    44401 aatcaatggt ccagttgttc ctagataaac tttcggtggc agtccagcat agttctgata
    44461 aggagatgag actgtaaggg tcaatttttt tccgatataa tttgctggta aatctatcac
    44521 agccaatgag agtccgggat tgctttcttc ctgataatct ttccgttggt ataaaatctt
    44581 ttcgcctaga tatacagtaa tccattgatg gtttcctttg attagtaaat ttgcttcgtc
    44641 caacggtttt tcaagtgtcg tttccataat gaatgtttcg tctttatcca cggtaggaat
    44701 cgtacgagtg tacttgctgt caaacttaaa atcaggatca tcttttgtat agaaaaccca
    44761 cgaatctttt atttctttca tttgattgct gtccgcagga gaaaaaataa aataaaagaa
    44821 ataagcaatt cctgttaaaa ataacaatga taatataatg attatgctcc actttatacg
    44881 taaataaaaa gattcttttt tattatatat gttattaaaa aaagacataa aggtattcct
    44941 ttctaaaact tgtaatgtta gggtaaacgt tttttaaact aacgtcaatt atttataata
    45001 aaaaatgaga taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat
    45061 ttttacagct ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt agtggaggaa tggggggctt tcaatgttga tgggtgaatt
    69601 ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact
    69661 tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct atccgatgag
    69721 cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg
    69781 tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata aacaaggacg
    69841 aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt gcgtagtcat
    69901 cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt tttctgcagc
    69961 agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac tataagaata
    70021 ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt gaaagaaacg
    70081 gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact cggcggtgcc
    70141 ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta tgcgtttgac
    70201 caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt
    70261 atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag tgagcatgta
    70321 gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct agatgaagca
    70381 gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga tcagtcagct
    70441 cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt aaagattttc
    70501 ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga acgagtacga
    70561 aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca agtcatccca
    70621 gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt
    70681 gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat ccggttattg
    70741 aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag aattgtcaaa
    70801 agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc tgtcatacct
    70861 gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc ttcagaggaa
    70921 gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga aaaaataaga
    70981 atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg aatatgattt
    71041 acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga atcaagaaat
    71101 cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg aaaaaattgc
    71161 tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac
    71221 aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca atgataaaaa
    71281 gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg aacggattaa
    71341 acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga aagtgaagta
    71401 aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa
    71461 caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt tctttttatt
    71521 tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct cattagctga
    71581 aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag gaacgattta
    71641 tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta aagcgatact
    71701 ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga attttgataa
    71761 aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac tgaatatttt
    71821 agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg ggaaaaatat
    71881 ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct
    71941 ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt ctcattttat
    72001 tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag gtttggagtc
    72061 tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa aagataattt
    72121 tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg gacaagatat
    72181 ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg atcaagtaaa
    72241 tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa ccggagaaat
    72301 cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat tgactggaaa
    72361 agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat caaccatgaa
    72421 agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg aaaccttcca
    72481 atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg gtgaaaaagg
    72541 tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg tcatactgga
    72601 acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg gacaaagtac
    72661 tcattctgga ttagatgatg aagtaaacgg tgctttacct acattaaata ttgtcgaccg
    72721 agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt
    72781 cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca aagtggtgga
    72841 tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac ctttttcaaa
    72901 agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg
    72961 tagtgcttat ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa acggaacagc
    73021 ccaaatcgct tctgatacag ggggatatca aacaggagat acagcctacc tgtattcgat
    73081 tgtggagatg gttccttctg aagatccaga ctatgtcttg tatctaacaa tgaaacaccc
    73141 taagacatat gatcgaatgg cattggctaa aatcgcaaat ccattgatga aacgggcaat
    73201 ggattttaaa gaaactgaag aagattcaga tacagaaaca aaaacagaaa aagtatccgt
    73261 agccgattat cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa aaagtggact
    73321 gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa caatctactg ccaacggtga
    73381 tcagttgatc tctggagaaa aactgatcct atatactggt ggtgataagc tgatgcctga
    73441 cgtaacagga tggtcaaaag cagatatcat gaagctgggg aaaatcctcg gtattgaagt
    73501 tagttttgat ggagatggtt attgtgtcaa acaagaactt gctccatatg aaaaaatcac
    73561 tgaagacaaa cttagtttta ctttagaaga ataagaagag aaataggaga gaaaagagca
    73621 tggaatggac acaagcattg attcccattg tcagcagttg tgcaatgaca attgccgcaa
    73681 tgccgctatt tattggttat tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg
    73741 gaccaaagtg gcacaatagc aaagccggaa ctcctacaat gggcggctta gtgtttctga
    73801 tcggctctat tttgacagga atttgggttg gagcttggca aaaacagcta actcctactc
    73861 ttttcatctt gctttttgta ttagcgttat acggagtaat cggctttctt gatgatttta
    73921 taaaaatatt caagaaaaga aacatgggac tgaactcgaa gcagaaattg attggacaga
    73981 ttctaggggg aatcatcttt tatcttgttt accgttctga aggttatcca ggtgtattga
    74041 atttcttcgg actagatcta cctttaggaa tcatttatgg tattttcgct ctcttctggc
    74101 ttgttggatt ttcaaatgca gtaaatctga cagatggcat cgatggatta gttgctggct
    74161 taggaagtat ctcttttgca gcttacggtc taattgcttg gcatcagcat cagtatgatg
    74221 tattaatcat ttgcttaagt gttttaggag gtttactggg tttcttcgtc tacaacagaa
    74281 aacctgccaa gatttttatg ggagatgtcg gttcattagc gttgggcgga cttttagcgg
    74341 caatttctat aatgctaaac caagaatgga cattgctttt gattggtttg atctatgtta
    74401 tggaaacagc aagtgtgatg cttcaagtta cttcctttaa gcttacagga aaacgaattt
    74461 ttaaaatgtc tccaattcat catcactttg aaatgtgcgg atggtcggag tggaagattg
    74521 atacagtttt ttggttgatc agcattgtga cttcgttgat cacattatgg atcgtttggt
    74581 aggagatgac gggaaatgaa aaaaatatca acatatgaaa ataagaaagt gcttgtctta
    74641 ggactcgcaa aaagcggagt aagtgcggcc aaattattac atgaattggg cgcacttgtc
    74701 accgttaatg atggaaaacc ttttgacgaa aatccggaag cacaagagct gctttcttta
    74761 gggataaaag taataacagg tagtcatcca atcgaattgt tggatgaaga attttcattg
    74821 atggtcaaaa atccagggat tccctacagt catcctttag tagcaaaagc ccaagaaatg
    74881 ggtattccgg taattacgga agttgagcta gcttacgaag tagcggaatg cccaattatt
    74941 gggattacag gaacgaacgg aaaaacaacg acaacaacga tgacaggact actactgaat
    75001 gcaggagcag atcaaggaat tgcacgttta gcgggtaata ttgggtatcc agcaagtggt
    75061 gtagcgcaag aagcaaaatc agaagataaa attgtgatgg aattgtctag cttccaactc
    75121 atgggaatca cagatttccg ccctcatata gcggtgatta ccaatatcta tgaagcacat
    75181 attgattatc atggtacaag aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac
    75241 atgacagaaa aagattatct gatccttaac tggaatcagt cagaactaca agaattagcg
    75301 caaagaacaa aagcacgcgt actgcctttc tcgacaaaag aagtgttgga agacggtgtg
    75361 tatgctgacg attactcaat atactataaa aaagaaaaaa taatggaaat atctgaactt
    75421 ggcgtaccag gtaagcataa tgtagaaaat gcactagctg ctatttctgt agcaaaattg
    75481 tatggtatat caaatgaagc tatacgagaa acgttgcatt ttttccatgg cgtaccacat
    75541 cgtacgcaat atgtaggaga aatccaaggc agaaaatttt ataatgattc aaaagcaacc
    75601 aatattttag cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg
    75661 gctggcggat tagacagagg aaatacattt gatgaattaa ttccttcatt aaaaggaatc
    75721 aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca
    75781 ggcatcgaga caatagtaac agccgattct gtagaacaag cagttccttt agcattagaa
    75841 aacagtacag atggtgatgt agttttatta tcaccagcga atgccagctg ggatcaatac
    75901 ccaaattttg aaactagagg aaacagattc atggaagctg tgaaccgatt aaaatagaaa
    75961 aagtagaagc gagcgataaa aaatgaaaat tttggttaca ggcggaggaa cagggggaca
    76021 tatttatcca gctcttgcct ttgtcaatta tgtaaagact caagaaccga atgctgaatt
    76081 catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc gtccctgata caggaatgcc
    76141 tttccatacc ttggagattc aaggatttaa acgaaaaatt tccatgcata acatcaagac
    76201 ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg attttcaacc
    76261 agatatcgtt atcggtactg gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa
    76321 aatgggtatt ccaacagtta tccatgaaca aaacagtgta ccaggtgtta ccaataaatt
    76381 tttaagccga tatgtggatc ggatcgctct ttcatttaaa gatgcggctt ccttttttcc
    76441 tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa gaagtagcag atactaaaaa
    76501 atcagatatt ttgaaaacat ttgatttgga tccagaaaag aaaacagtct tgatttttgg
    76561 cggtagtcaa ggagcactta aaatcaatca agcagtatca gtttttttaa gtgaatatat
    76621 tccttcttct taccaagttt tgtatgcttc aggagataga tattatgaag agatcctaaa
    76681 taaaataaat cctgaaatac aaaatattag tgtccagcct tatatcaaaa atatggcaga
    76741 ggtcatggct tccagtgatc tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt
    76801 cacagctctg gggttaccgg cagtgttggt tcccagccca tatgttacga atgatcatca
    76861 gactaaaaat gcaatgagtc ttgttcacgc aggagcagca aaaatgattg cagataatga
    76921 actaacaggt gaatcactaa gtcagacagt caacgaaatc atgggtgatg aagaattgca
    76981 aaagcaaatg tgccgagctt ctaaagaaca aggaatccct gatgcttcaa aacgtcttta
    77041 tgacttagta aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa
    77101 gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca atttgactcc ttggcaaaag
    77161 gctaaccgaa aatatctggc agaacaagag atgaaaaacc aagagccaaa aaaagaagac
    77221 ataacattag atacacccct ttcagattct gaagaagaaa aaacggttca agaggaacag
    77281 caagaagaaa gccagtccga gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa
    77341 aacggtggac catataacgg ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg
    77401 cacaaagtac tatttcgtcg gttagctttg atcatcactg tattgggtat accgctgatt
    77461 tttttgatct attatgtttc tccttatagc aaattacaag cagttgcagt atcaggcaat
    77521 aagacagtca acagccaaga aatcatctct gatacaaaat tgtcattagg agaaaatgtt
    77581 tggggacagt attttcatcg atctacttat atcgatcgcc ttaaaaaagc acagccaaga
    77641 atcgaaacag caaatataca tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa
    77701 tataaagaga ttgctttgat cgcaaaaaac aatcaatact acccagtgat tgaaaatgga
    77761 acagtattag atgaaaaagt agcaaatcct acaaaaaatc tccctatctt ggaagagttc
    77821 aaagactctg caaaaatcaa agaattaaca aagcaataca atcagttatc atcagaatta
    77881 caaaaagcaa tttctgaaat caagtacacg ccaagagcaa gcaataaaaa tctgatccaa
    77941 ttgaatatga atgacggcaa tcaagttatt gtaaatatca ataatcttgc gaaccaaatg
    78001 aaatattatt cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga catggaagtt
    78061 ggtatcttct cgcatcctta cggtagcact caagaaacgg aagaatcggg ccaatcggaa
    78121 gaaacaactg aaagtgaaga aaatgcggat caaacaaacg aatcaagtga agaggcagaa
    78181 acgcaaaaag cacaaacacc tggatcgtct agcacaaacg gatcatctga ttcaaatgat
    78241 tcaagtgaaa caagtcaaat agaagataat ccaagtacaa ctccgtcaag tttaaatgaa
    78301 aattgataaa acaactttaa aaagtaagaa tgtactttct aaaaaccagt aatttatggt
    78361 agaattggaa gcagtgaata tatctattaa taagtatatt ttaaaacgga cgctagttgt
    78421 tcgcttgtat atggaatagt aagtaggagg gaatcccatt catggcaaaa acaggaatgt
    78481 atgtaggcct tgatattgga acgacatctg ttaaagttgt cgtggctgaa tatatcgaca
    78541 gccaaatgaa tattattgga gtaggaaacg caaaatcaga agggattaac cgaggcatta
    78601 tcgttgatat cgataaaacg gtccaagcga tacaacgagc agtacgacaa gcagaagaaa
    78661 aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag
    78721 aaaattgcca gggaatgatt gcagttaacg gagattcaaa ggaaatcacc gatgaagatg
    78781 tcagaaatgt ggcttcagct gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt
    78841 caatattgcc acaagatttt actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa
    78901 tgatcggcgt aagattagaa atgtacggtc tgttgtttac aggacctaaa acgatcatcc
    78961 ataacatacg taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg gtgattactc
    79021 ctttagcgct gactgaatca attctttctg acggagaaaa agattttggt acgatcgtca
    79081 ttgatatggg tggcggccaa acaacaacgg ctgttatgca tgataagcaa ttgaagttta
    79141 ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga tatctctatt gtattaaata
    79201 cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg ggatgcatac ccagaacgga
    79261 catcaccaga tgaagaattt ccagtagatg tgattggtca gtcagaacca gtgaaagtcg
    79321 atgaacgcta cttgtcagaa attatttcag ctcgaatgga acagattttc aacaaagcaa
    79381 aagatgcatt ggaccaaatc gaagcattag agttgccagg tggcgtcgta ttgacaggcg
    79441 gagctgctag tcttcctggt gttgtggact tggcacaaga aatcttcggc gtaaatgtaa
    79501 aattgtatgt tccaaatcac atggggctga gaaacccggt atttacaaat gtaataagta
    79561 tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg
    79621 gtgaaacctc agcagctcgt caaatggtag ttgaacaaga aacaacaaac acatatgaga
    79681 gctatgaagc acctgaagaa acaatctacg atgagccgga accaaaagag tccggagaaa
    79741 atgtaaaaaa taaaatcaag ggtttcttta caaatatttt tgattaattc atcctgggag
    79801 gaataagata tggaattctc aattgataat aatatcaatg acggcgcagt aatcaaagtc
    79861 atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa
    79921 ggtgttgaat tcattacagc caacacagac gtgcaagcat taaaaaattc aaaagctgaa
    79981 acagttatcc aattaggccc caaatatact cgtggtttag gtgctggttc tcagccagaa
    80041 gttggacaaa aagcggcaga agaaagtgaa caatccttac gcgaagcttt agacggtgca
    80101 gatatgattt tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc
    80161 gttgcaggta tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac acgtccattc
    80221 acttttgaag gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa
    80281 aatgtggata cattattaat tatctcaaat aaccgtttat tagaagttgt agataaaaag
    80341 acaccgatgc ttgaagcttt ccgtgaagca gataatgttt tacgacaagg tgtacaaggt
    80401 atctctgatt tgatcacagc gccaggttac gttaaccttg actttgctga cgtgaaaaca
    80461 gtgatggaaa accaaggtac agcattgatg gggatcggtg tggctagtgg agaagatcgc
    80521 gtagtagaag caacgaaaaa agcaatctcc tctccattgc ttgaaacatc tattgatgga
    80581 gctgaacaag tacttcttaa catcacaggc ggtttggata tgacattgtt cgaagcacaa
    80641 gatgcttcag atatcgtagc aaatgcagca actggcgatg taaatatcat tctaggtaca
    80701 tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat
    80761 gaatcaaaaa aggaacgcaa gtctagccga ccagctagac aagcacaaat gcaatcacct
    80821 gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa cttcttcaga agaagaaaac
    80881 agttttggcg attgggatat cagacgtgaa caaaatgttc gtcctagagt tgatgattcc
    80941 aattttgaca atatcgaaaa aaaagaattt gatacattca accgcgaaga aacaaaatct
    81001 catggcgatg atgaattaaa tacgccaccg tttttccgtc gtaaaaaata aaggagagga
    81061 agagcacatg attgctgaca acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc
    81121 tcttgttcat cgtgatccga aagaagttac attagtagct gtaacaaaat ctgttgaatc
    81181 cgctcaagct gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa accgagtaga
    81241 taaattatta gaaaagaaac aagatttatt tgattttcct gaaataaagt ggcatttaat
    81301 tggtaattta cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt actttcatgc
    81361 gttggacagt ttaagattag cagaagaaat ccagaagcgg ttagagaaag aattatcctg
    81421 ttttattgaa gtaaatgttt ctggtgagga atcaaaacat ggaatttcac cagatgagct
    81481 tttaccgttt gtggaatctt tagctgcctg cacaaaaatc aaggttgttg ggttgatgac
    81541 tatggcgcct aaagaagctt ctgatgagca aatccataaa gtgtttggta cgctgaaaga
    81601 attgcgtgat cttgttcaag caaaaggtta tgcccatgca ccatgtagtg aattaagtat
    81661 ggggatgagt caagactttc aaattgcgat tgaagaaggt gcaacattta tccgtgtagg
    81721 aacagcgcta ttcaaagacg aataaaggag gaacaaaaat gccgctttta aacagaggag
    81781 caattgctaa ttttttcgga ttagctgatg aggaagagta cgaataccaa gattatcctg
    81841 aaacaacaaa aaaggctgta cagccaacta aaaaaaatac aacacagaat caatcaactc
    81901 attctgtatc tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa
    81961 cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat tgaactacat caatcaccta
    82021 caaaaactgt agcgaaagaa agaacagctt caagaacctc tacacaacct aaagcaaatc
    82081 ttgctggaaa aatcactgta atggaaccaa gagcttattc tgaagctatg gcgattgcta
    82141 aacgaatcat tgctggagaa gcaaccttag tgaattttca tttagtagaa gaaaaacaag
    82201 cgagaagaat cgttgatttt ctaacaggaa cagtttatgc actagatgga gatatcaaac
    82261 gtgttggaga tgagattttt ctttgtacac cgactgggat ggagattgac agttcaaccg
    82321 ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga ggaggaacta cgatttatac
    82381 tattgcacta ttacttcatt tacttcaacg ggcagcctat ttctatagca ttatcttgat
    82441 ggtatatgca ttgctctctt ggtttccagg cgggtatcaa tcagggctag gacgattatt
    82501 agctaagatc tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga ggattggacc
    82561 cttggatttc acaattttag gggcaatcct gatcttgaac ttagccgtac aaggtttagc
    82621 acagatcgtt atatggataa ctgtccattt agtttattga ttgaagcaac aaaatcttta
    82681 ttctcagaat agataggagg ggaaaatttt ggatgcaaat gtataccagc actttcgggc
    82741 agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc aaggacaata
    82801 tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag aaacactgat
    82861 tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa
    82921 aagatgtttg attttccccg attattacga acctgtagag gaagattttg aaacagcatt
    82981 gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa tccttggaac
    83041 tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg acggtgatcg
    83101 ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag taacaaagat
    83161 tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta tgccgaaaga
    83221 tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg tgatctctga
    83281 gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta aagtaaaagt
    83341 gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata ttatttcagt
    83401 cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa aagaaaagta
    83461 cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact catggcttta
    83521 actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata taatcaagac
    83581 gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac tcaaagaaat
    83641 cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt caatgaatta
    83701 aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa agtaaaaaca
    83761 agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc agatgagtta
    83821 gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga
    83881 aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac tgaagatctt
    83941 aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc tcaattagaa
    84001 caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta cgtgactgac
    84061 agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa tgatactgat
    84121 gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac acaggctatt
    84181 gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc actttgttat
    84241 tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg aaagatcctc
    84301 tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct cgttattggt
    84361 cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg gcatgttcgt
    84421 cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt gtatggttga
    84481 atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg tggtaatttg
    84541 ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga
    84601 caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc atatgccaat
    84661 ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat cattcgttca
    84721 aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca tggattgccg
    84781 attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac
    84841 ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc agatttcaaa
    84901 cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc ttcttatgaa
    84961 gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta caaaggattg
    85021 aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga aattgaatat
    85081 aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg caaagatatt
    85141 cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt gccagcaaac
    85201 caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga tggccgtaag
    85261 tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg ggaaaatgta
    85321 acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag tcatccattc
    85381 tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc tggtactgga
    85441 ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag caaatataaa
    85501 ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc acctggattt
    85561 gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa agaaaaagga
    85621 gctttgctga agctagattt ctttactcat agttatcctc acgattggcg gacgaaaaag
    85681 cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt ccgtcagaat
    85741 attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac acgtttgtat
    85801 aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg gggagtacca
    85861 cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac aatcgaacat
    85921 gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga agcgaaagaa
    85981 cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa
    86041 gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt acgtcagcga
    86101 ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata tcgtggctgg
    86161 ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata caaatcagtt
    86221 atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac
    86281 atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg cttatgggta
    86341 tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag tcaagtggca
    86401 gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac agaagatttc
    86461 gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa atatatgacc
    86521 gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa atataacttc
    86581 atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc attctacctg
    86641 gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg tcgttgcatg
    86701 caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc tatcattccg
    86761 catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt tcagttaagc
    86821 gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac ctggactgct
    86881 ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg
    86941 attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt tcgtgaaatg
    87001 ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga ctactttgaa
    87061 gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga tgttgctatt
    87121 acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg taaagatgta
    87181 ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc
    87241 tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca
    87301 tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt cacgatacca
    87361 gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga aagctgcttt
    87421 aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt cccaagcttt
    87481 tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag agaagttcgt
    87541 tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc tattttcaac
    87601 catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc cttgccctac
    87661 ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga tcgttagaac
    87721 attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga atacattaac
    87781 aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac gtttgcctgt
    87841 acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac
    87901 gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc ccttgaattg
    87961 tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta acacttcttc
    88021 ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga tttctttttc
    88081 ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa tatgattttg
    88141 caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt ctacaccgag
    88201 gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt gagcctcaaa
    88261 aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc ctaaataatg
    88321 atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt tcaattcaaa
    88381 agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga agccattttt
    88441 tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat
    88501 agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa
    88561 ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat agtaaaaatg
    88621 gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg tttctttgac
    88681 aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta cagcaaaatg
    88741 ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc
    88801 taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag acatgtggat
    88861 acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct gacggggatt
    88921 ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc aacgatctca
    88981 tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc tttttcatca
    89041 agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt ttgtcggcgt
    89101 tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca atgttcagga
    89161 tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg ttccaatact
    89221 tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa aaaaacttcc
    89281 cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc agtcaattgt
    89341 acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga aatcatttca
    89401 ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt gactttcgtt
    89461 gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg tgtcatattc
    89521 gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg
    89581 actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa aaaacgaaag
    89641 atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt gggagggatc
    89701 tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag agtttgttcg
    89761 ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg atattcatct
    89821 atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta atcagatgta
    89881 atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg atactttgag
    89941 agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact tgagagaacg
    90001 gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact cctttatgag
    90061 aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat aaaagctaaa
    90121 atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat gataggatac
    90181 atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag ctttttttat
    90241 tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa taattattgc
    90301 gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg aagtggctat
    90361 tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg taaaaaagaa
    90421 gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg atggtatttt
    90481 agacggcacg atccttttac caggattaac tggtccattt acggtataca agcgtgaagt
    90541 aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac aaaaagcatt
    90601 tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc
    90661 cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag atggttatgc
    90721 cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga tccatgaaga
    90781 aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag ttcaaatcct
    90841 cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca ttccatttga
    90901 tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa tgatcaactt
    90961 aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt taagcacaaa
    91021 aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg aatttggttc
    91081 tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca atattactca
    91141 tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag ctaagcaagc
    91201 aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc caaaagaaat
    91261 ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag cagcaagaaa
    91321 tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag acgagataat
    91381 cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat tagatggaga
    91441 agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt taggtgttta
    91501 ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt atttcccaag
    91561 aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg agaattttga
    91621 tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga tttattttga
    91681 ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat ttcatgatcg
    91741 cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa tggcagatga
    91801 agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg gattgttgta
    91861 tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa
    91921 tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga atttgtacaa
    91981 gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc accttctctt
    92041 attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc ggtttctcct
    92101 acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt ggaaccaggt
    92161 gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca agtagcagat
    92221 gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt gggcggagcc
    92281 aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat tggtacacca
    92341 ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg
    92401 gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat tgatcaaatt
    92461 cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc agatcgttcc
    92521 cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt gacagcagag
    92581 gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg aaagaaagat
    92641 gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt ctttaatcaa
    92701 ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt
    92761 ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt caaaaagaaa
    92821 atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat tacaggtctt
    92881 ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca tcgttcggga
    92941 agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga taataatcta
    93001 agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat ttttttatac
    93061 ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc agtaaataaa
    93121 aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa agaaaaaata
    93181 agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa
    93241 gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt
    93301 atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga
    93361 cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa
    93421 gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt
    93481 tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt
    93541 ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg
    93601 cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga
    93661 agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag
    93721 atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg
    93781 gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc
    93841 taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg
    93901 aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa
    93961 gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa
    94021 gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt
    94081 cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc
    94141 gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg
    94201 cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt
    94261 tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc
    94321 aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg
    94381 acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag
    94441 atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg
    94501 aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt
    94561 tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg
    94621 aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt
    94681 ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga
    94741 gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg
    94801 aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc
    94861 cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc
    94921 ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa
    94981 gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc
    95041 cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc
    95101 aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca
    95161 gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca
    95221 tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc
    95281 aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg
    95341 aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg
    95401 ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga
    95461 tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg
    95521 agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag
    95581 cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgacgaga
    95641 tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg
    95701 atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta
    95761 tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat
    95821 tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc
    95881 gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa
    95941 aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa
    96001 aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag
    96061 agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt
    96121 atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta
    96181 gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa
    96241 gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta
    96301 gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa
    96361 tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat
    96421 ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag
    96481 tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt
    96541 taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa
    96601 tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag
    96661 cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga
    96721 cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag
    96781 caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt
    96841 gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt
    96901 tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa
    96961 atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga
    97021 tgcttttatc ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc
    97081 aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga
    97141 caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg
    97201 tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc
    97261 ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat
    97321 ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga
    97381 tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt
    97441 atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat
    97501 gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat
    97561 gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat
    97621 cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact
    97681 gatcattgat ttggtgcaaa aatatattgc agagccattc ctaggattat ctcaagggtt
    97741 gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg
    97801 accaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat
    97861 tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat
    97921 cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg
    97981 cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga
    98041 ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt
    98101 gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc
    98161 agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc
    98221 gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga
    98281 ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc
    98341 atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg
    98401 tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa
    98461 tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca
    98521 atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa
    98581 caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc
    98641 cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata
    98701 ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt
    98761 tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt
    98821 ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg
    98881 agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga
    98941 taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag
    99001 aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat
    99061 aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa
    99121 tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta
    99181 cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac
    99241 aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac
    99301 gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg
    99361 aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg
    99421 ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt
    99481 gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt
    99541 acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt
    99601 caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc
    99661 attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg
    99721 gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc
    99781 tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa
    99841 ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat
    99901 tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga
    99961 gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac
   100021 cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt
   100081 gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc
   100141 cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc
   100201 tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca
   100261 ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa
   100321 aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa
   100381 ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca
   100441 aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt
   100501 aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg
   100561 ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg
   100621 ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt gaaggaaaat
   100681 gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt gttttagggt
   100741 tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt tttttagatg
   100801 aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga aaggacctcc
   100861 ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg aaacgttttg
   100921 cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac aaacaagcta
   100981 caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg cctcctaagc
   101041 gataggtcgc tggttcaatt ccagtcagga acgtacaaat aacccctgta tcctttgcgg
   101101 tacagggggt tattttgtat aagtagacat tgtagtttct aaactgctat gtcctaaacg
   101161 tttcgatacg ctaagtatat ttactccttg ataaagtaaa atagatgcat gcgtatgtct
   101221 tagtccatca ataaacagtc gaagagaaaa tagggaaaaa ttgattacta aagaaaaact
   101281 tactaggtcg acggtgtagt tgattttcat acttcaatag attcgaatta aacgatttcg
   101341 attggcttat accagtccta tccaacgaaa gatatatcgt tcttaactct agcggtataa
   101401 cttgaagctt tacgtaaagc caatgggaat gaactggaga tgacttttta ttatttttag
   101461 taatagctga tgggttaata cttacaactt tccttttcaa agtaccatat aaaaaaacgg
   101521 tggggttgtt tagcgaaata aaggatctaa gccaatagct tagatccttt attctatgct
   101581 ttatattatt ttttgcatgt gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa
   101641 tcggtactgt agagccatct tcacggattc cgtcgatatc catcttgtca gatcctacca
   101701 tgaaatctac gtgggtttga cttctattca atccagcttc tgctaattct tcatcggaca
   101761 tttctgtacc gcctttcaca ctgaaggcat aagcagaacc tagtgctaag tgattcgatg
   101821 cgttttcatc aaataatgta ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt
   101881 caggtacaag tgcgacttct cctaaacgac gcgcaccttc atctgtatca agcagttttg
   101941 ctaagacatc ttctccttgt tcggcagaaa aatcaacaac ttttccatct ttaaaagtaa
   102001 atttcatacc cgagatgatg gtgccagcat aactcagtgg ctttgtactt gaaatgtaac
   102061 catctactcg atgactgtca ggtgctgtga acacttcttc cgtcggcata tttgccatga
   102121 atttttctcc acgagcatta tagcttccag cgccttccca aagatggttt ttaggcaatc
   102181 cgataatgat atctgtaccg ggagcagtat agtgaagggc agaaaattgt tctcgattca
   102241 attcttctgc tttttttgct aatttttcat catgcttctt ccatgctaag acagggtctt
   102301 cttcatagac acgagtcgtt ttgaaaatct gatcccataa tgcctcaact tgtttttctt
   102361 ctggcagttc tgggaagact tttgctgccc attgtttacc agccgcagcc acgacagtcc
   102421 aactgacttt atttgcttga gttgccttgc gtaaattcat tagtgctttg ccattcgctg
   102481 attgataaga cgctactcga tggctatcta caccagcaaa tgcatcggga tcggcagaaa
   102541 caacgctgat cctgcttgct cctttttcaa gccattcatc cgcttgatcg attttagatt
   102601 gtggtacatt ctcaatacga tccgttgcag catgtaaaag aaattctctt tggatctggt
   102661 cgtcagtcca ttgaacgata acctcagcgg ctcctagttt ataagcttct tgtgtgatca
   102721 atcgagcaag gggtgcctgt tccacgctga tctgtaaaac aactgtgtgt cctttttctg
   102781 tggctacgcc agtttctgca attaagcgtg catatttttt tagaagttca ttaaaatcag
   102841 ataacataat aaaaattcct cctcaccatt ttttctggta actattagca tgataccatt
   102901 taattatgat tcaaacaatg aggctttaaa taaaaaaagg aggttatttt tttaaggtta
   102961 agtttcatga tatatgttac tacttgctat ttcagcttgt gaagaaaatc aaaaactaat
   103021 tttgcatatc aaaaaaacat gttacaatga aattaatcaa tggtggaagc gttataaaaa
   103081 aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa cagattttgg
   103141 cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg agaaacatcg
   103201 cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat atggatgact
   103261 tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg ttcactgttg
   103321 agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt gcaagaagag
   103381 agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa gagatgcaga
   103441 atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat gtgaatcttt
   103501 cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact gggatcgcag
   103561 ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag atgatccaag
   103621 acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga atatactcaa
   103681 aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc
   103741 ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat gaataaaaga
   103801 gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta aatttttgcg
   103861 actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc attcagatcc
   103921 actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc atcgaaatca
   103981 tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac accgtaaatt
   104041 tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc atcaggcaga
   104101 tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc gaattcatct
   104161 gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc aaatgcttct
   104221 gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac gatttttgct
   104281 aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta taccaaaaca
   104341 tcattgaaaa gaaaataaaa cctaaagaca cctggggaat gtcatttggc taaatgtatg
   104401 ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat tactttaaaa
   104461 tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga tgtacataaa
   104521 aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt atttgtcaga
   104581 aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg gtatgaatat
   104641 gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg
   104701 aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg catttctgat
   104761 tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt gatggaagta
   104821 acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg gattggtgac
   104881 atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt
   104941 attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca ttacggagaa
   105001 gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc tatggttaat
   105061 accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac aagagacggc
   105121 ggactttctt gccaatatga acatacattg gctattacta aagatggtcc aagaattttg
   105181 acatcacaag gagaagaagg cacttattga tctatccagt caataggtac gggagaggta
   105241 catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac
   105301 tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg cttattattt
   105361 actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt ttctccacat
   105421 cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg tttaccagtt
   105481 tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt tatccgtttc
   105541 tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa
   105601 taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct tctcactaat
   105661 tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg ggttgggaca
   105721 gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta tcgatcagtt
   105781 ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt tcttgattta
   105841 tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca cgatcttcgc
   105901 tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt attttgcaac
   105961 gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt ggcttaattt
   106021 tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag aatacgtgac
   106081 gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata aattgaaaac
   106141 taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt atatcagtaa
   106201 tttataa
//
LOCUS       DAJSBM010000004        87927 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJSBM010000004 DAJSBM010000000
VERSION     DAJSBM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.004"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(552..2165)
                     /locus_tag="ONT67_000340"
     CDS             complement(552..2165)
                     /locus_tag="ONT67_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2354106.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="ONT67_000341"
     CDS             complement(2306..3214)
                     /locus_tag="ONT67_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354107.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="ONT67_000342"
     CDS             3397..4521
                     /locus_tag="ONT67_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2354108.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4546..4725
                     /locus_tag="ONT67_000343"
     CDS             4546..4725
                     /locus_tag="ONT67_000343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354109.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            4748..5665
                     /locus_tag="ONT67_000344"
     CDS             4748..5665
                     /locus_tag="ONT67_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354110.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="ONT67_000345"
     CDS             5658..6491
                     /locus_tag="ONT67_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2354111.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="ONT67_000346"
     CDS             6574..7692
                     /locus_tag="ONT67_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2354112.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="ONT67_000347"
     CDS             7686..9584
                     /locus_tag="ONT67_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCR2354113.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQISEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="ONT67_000348"
     CDS             9578..10801
                     /locus_tag="ONT67_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2354114.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="ONT67_000349"
     CDS             complement(10887..12821)
                     /locus_tag="ONT67_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2354115.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="ONT67_000350"
     CDS             13047..13703
                     /locus_tag="ONT67_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2354116.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="ONT67_000351"
     CDS             complement(13777..14130)
                     /locus_tag="ONT67_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2354117.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="ONT67_000352"
     CDS             14322..15251
                     /locus_tag="ONT67_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2354118.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="ONT67_000353"
     CDS             15262..16098
                     /locus_tag="ONT67_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCR2354119.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="ONT67_000354"
     CDS             16346..17122
                     /locus_tag="ONT67_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2354120.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="ONT67_000355"
     CDS             17298..18041
                     /locus_tag="ONT67_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354121.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="ONT67_000356"
     CDS             18034..19614
                     /locus_tag="ONT67_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR2354122.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="ONT67_000357"
     CDS             complement(19865..20344)
                     /locus_tag="ONT67_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2354123.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="ONT67_000358"
     CDS             complement(20360..21112)
                     /locus_tag="ONT67_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCR2354124.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="ONT67_000359"
     tRNA            21229..21299
                     /locus_tag="ONT67_000359"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="ONT67_000360"
     CDS             21660..22385
                     /locus_tag="ONT67_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2354125.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="ONT67_000361"
     CDS             22640..22906
                     /locus_tag="ONT67_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCR2354126.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="ONT67_000362"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="ONT67_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR2354127.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="ONT67_000363"
     CDS             23359..23664
                     /locus_tag="ONT67_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCR2354128.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            24233..25070
                     /locus_tag="ONT67_000364"
                     /pseudo
     CDS             24233..25070
                     /locus_tag="ONT67_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            25135..25569
                     /locus_tag="ONT67_000365"
     CDS             25135..25569
                     /locus_tag="ONT67_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2354129.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(25637..26959)
                     /locus_tag="ONT67_000366"
     CDS             complement(25637..26959)
                     /locus_tag="ONT67_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCR2354130.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27113..28753)
                     /locus_tag="ONT67_000367"
     CDS             complement(27113..28753)
                     /locus_tag="ONT67_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCR2354131.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            29083..29553
                     /locus_tag="ONT67_000368"
     CDS             29083..29553
                     /locus_tag="ONT67_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCR2354132.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            30182..31285
                     /locus_tag="ONT67_000369"
     CDS             30182..31285
                     /locus_tag="ONT67_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2354133.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            31569..32249
                     /locus_tag="ONT67_000370"
     CDS             31569..32249
                     /locus_tag="ONT67_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2354134.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            32339..33373
                     /locus_tag="ONT67_000371"
     CDS             32339..33373
                     /locus_tag="ONT67_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2354135.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            33387..33722
                     /locus_tag="ONT67_000372"
     CDS             33387..33722
                     /locus_tag="ONT67_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354136.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            33706..36186
                     /locus_tag="ONT67_000373"
     CDS             33706..36186
                     /locus_tag="ONT67_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354137.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            36242..37801
                     /locus_tag="ONT67_000374"
     CDS             36242..37801
                     /locus_tag="ONT67_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354138.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            37798..39162
                     /locus_tag="ONT67_000375"
     CDS             37798..39162
                     /locus_tag="ONT67_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2354139.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            39333..40403
                     /locus_tag="ONT67_000376"
     CDS             39333..40403
                     /locus_tag="ONT67_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354140.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            40418..41086
                     /locus_tag="ONT67_000377"
     CDS             40418..41086
                     /locus_tag="ONT67_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354141.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            41106..41684
                     /locus_tag="ONT67_000378"
     CDS             41106..41684
                     /locus_tag="ONT67_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2354142.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            42087..42998
                     /locus_tag="ONT67_000379"
     CDS             42087..42998
                     /locus_tag="ONT67_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2354143.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43254..44171)
                     /locus_tag="ONT67_000380"
     CDS             complement(43254..44171)
                     /locus_tag="ONT67_000380"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCR2354144.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            44373..44810
                     /gene="zapA"
                     /locus_tag="ONT67_000381"
     CDS             44373..44810
                     /gene="zapA"
                     /locus_tag="ONT67_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR2354145.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            44868..45398
                     /locus_tag="ONT67_000382"
     CDS             44868..45398
                     /locus_tag="ONT67_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCR2354146.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            45530..47890
                     /locus_tag="ONT67_000383"
     CDS             45530..47890
                     /locus_tag="ONT67_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCR2354147.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            47977..48291
                     /gene="trxA"
                     /locus_tag="ONT67_000384"
     CDS             47977..48291
                     /gene="trxA"
                     /locus_tag="ONT67_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2354148.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            48473..50251
                     /gene="uvrC"
                     /locus_tag="ONT67_000385"
     CDS             48473..50251
                     /gene="uvrC"
                     /locus_tag="ONT67_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR2354149.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            50396..51295
                     /locus_tag="ONT67_000386"
     CDS             50396..51295
                     /locus_tag="ONT67_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354150.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            51288..52550
                     /locus_tag="ONT67_000387"
     CDS             51288..52550
                     /locus_tag="ONT67_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354151.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(52644..53936)
                     /locus_tag="ONT67_000388"
     CDS             complement(52644..53936)
                     /locus_tag="ONT67_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2354152.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            54220..56118
                     /locus_tag="ONT67_000389"
     CDS             54220..56118
                     /locus_tag="ONT67_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCR2354153.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(56417..57136)
                     /locus_tag="ONT67_000390"
     CDS             complement(56417..57136)
                     /locus_tag="ONT67_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCR2354154.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            57473..57805
                     /locus_tag="ONT67_000391"
     CDS             57473..57805
                     /locus_tag="ONT67_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354155.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            57792..59795
                     /locus_tag="ONT67_000392"
     CDS             57792..59795
                     /locus_tag="ONT67_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCR2354156.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            59814..60284
                     /locus_tag="ONT67_000393"
     CDS             59814..60284
                     /locus_tag="ONT67_000393"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCR2354157.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            60305..60889
                     /locus_tag="ONT67_000394"
     CDS             60305..60889
                     /locus_tag="ONT67_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCR2354158.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            60904..61890
                     /locus_tag="ONT67_000395"
     CDS             60904..61890
                     /locus_tag="ONT67_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCR2354159.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            61880..62191
                     /locus_tag="ONT67_000396"
     CDS             61880..62191
                     /locus_tag="ONT67_000396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCR2354160.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            62213..63994
                     /locus_tag="ONT67_000397"
     CDS             62213..63994
                     /locus_tag="ONT67_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCR2354161.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            63987..65363
                     /locus_tag="ONT67_000398"
     CDS             63987..65363
                     /locus_tag="ONT67_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCR2354162.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            65370..66005
                     /locus_tag="ONT67_000399"
     CDS             65370..66005
                     /locus_tag="ONT67_000399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCR2354163.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            66035..67390
                     /locus_tag="ONT67_000400"
     CDS             66035..67390
                     /locus_tag="ONT67_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCR2354164.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(67582..68976)
                     /locus_tag="ONT67_000401"
     CDS             complement(67582..68976)
                     /locus_tag="ONT67_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2354165.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            69068..70039
                     /locus_tag="ONT67_000402"
     CDS             69068..70039
                     /locus_tag="ONT67_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCR2354166.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            70036..70818
                     /locus_tag="ONT67_000403"
     CDS             70036..70818
                     /locus_tag="ONT67_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCR2354167.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(70987..71358)
                     /locus_tag="ONT67_000404"
     CDS             complement(70987..71358)
                     /locus_tag="ONT67_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2354168.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            71492..71935
                     /locus_tag="ONT67_000405"
     CDS             71492..71935
                     /locus_tag="ONT67_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCR2354169.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(71978..72787)
                     /gene="yidA"
                     /locus_tag="ONT67_000406"
     CDS             complement(71978..72787)
                     /gene="yidA"
                     /locus_tag="ONT67_000406"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR2354170.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(72806..74173)
                     /locus_tag="ONT67_000407"
     CDS             complement(72806..74173)
                     /locus_tag="ONT67_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2354171.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(74157..74987)
                     /locus_tag="ONT67_000408"
     CDS             complement(74157..74987)
                     /locus_tag="ONT67_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCR2354172.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            75150..75587
                     /locus_tag="ONT67_000409"
     CDS             75150..75587
                     /locus_tag="ONT67_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCR2354173.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            75663..76283
                     /locus_tag="ONT67_000410"
     CDS             75663..76283
                     /locus_tag="ONT67_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCR2354174.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            76472..76978
                     /locus_tag="ONT67_000411"
     CDS             76472..76978
                     /locus_tag="ONT67_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCR2354175.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            76995..77894
                     /locus_tag="ONT67_000412"
     CDS             76995..77894
                     /locus_tag="ONT67_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCR2354176.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            77981..78865
                     /locus_tag="ONT67_000413"
     CDS             77981..78865
                     /locus_tag="ONT67_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR2354177.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(79057..79128)
                     /locus_tag="ONT67_000414"
     tRNA            complement(79057..79128)
                     /locus_tag="ONT67_000414"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79093..79095),aa:Arg,seq:ccg)
     gene            79188..80522
                     /gene="rpoN"
                     /locus_tag="ONT67_000415"
     CDS             79188..80522
                     /gene="rpoN"
                     /locus_tag="ONT67_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR2354178.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            80671..81762
                     /locus_tag="ONT67_000416"
     CDS             80671..81762
                     /locus_tag="ONT67_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCR2354179.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            81803..82804
                     /gene="gap"
                     /locus_tag="ONT67_000417"
     CDS             81803..82804
                     /gene="gap"
                     /locus_tag="ONT67_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2354180.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            82914..84104
                     /locus_tag="ONT67_000418"
     CDS             82914..84104
                     /locus_tag="ONT67_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR2354181.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            84208..84963
                     /locus_tag="ONT67_000419"
     CDS             84208..84963
                     /locus_tag="ONT67_000419"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR2354182.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            85079..86377
                     /gene="eno"
                     /locus_tag="ONT67_000420"
     CDS             85079..86377
                     /gene="eno"
                     /locus_tag="ONT67_000420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR2354183.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            86776..87915
                     /locus_tag="ONT67_000421"
     CDS             86776..87915
                     /locus_tag="ONT67_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCR2354184.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        28900 a        15226 c        18141 g        25660 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgccaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ctgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cggcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg
    23701 attgtgacaa acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc
    23761 ccctcggcat tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc
    23821 taataatcag gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca
    23881 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt
    23941 ccttgtgact cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
    24001 cttgcatctg cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg
    24061 ctgttcttat tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa
    24121 tttccttaaa aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa
    24181 agaaaagagt aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg
    24241 tctgatttat tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg
    24301 tggaggcgtg ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat
    24361 ttctttttac tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt
    24421 atcaagcggg aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt
    24481 tggaggagta gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa
    24541 gcatgtgcag ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta
    24601 caccaagcat catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg
    24661 ggggggttct gggcttttta gctagttttt taggtattgg tggtggacca gtcaatgttt
    24721 ctttgttgat gctgatgttt gcattgccaa taaaagaagc aacactatat tctctatcta
    24781 cgattttctt ttctcaactg gcaaaattgg taacgattgc tttaacaagt agctttatgc
    24841 gctttgattt atctatgttg ttttatgtta tccccgctgc tgttattgga ggtttgtggg
    24901 gagcaagatt tagcagggtt ttgtctccaa aaaaagtgac ctttattttt caagtaattg
    24961 tcctcgtggt tcttttggtc aattgttaca atgcctttgt catcttacaa acattaggag
    25021 aatctagact aacgcaagtc aaaatcactg aaacatattg ttctaaatga aaaaaagagt
    25081 atactatgat aaaaaaattc attttaaaaa atcagaaatt ggctggtgat ttggatgtta
    25141 cgggaaatgc gtttagaaga tgcagaagca ctacgtgaaa tcaatgcgga tcaattaggg
    25201 tatgatatac cattaaccgt tacggtgaga caaatgaaga aactgctgaa ggagccagag
    25261 aaaaattttt tcttggttta tgaagatcct acttctaaaa aagcagctgg ctatgtacaa
    25321 gcggagtcct atgaatcgat ttttagcgat ccaatgttca atattatggc acttgctgtg
    25381 tctaaatatg ctgaaaaaag aggcatcgga aaggctttga tgactggcgt agaagaagaa
    25441 gcgaaaaaac gaggaataac gacaataagg cttaattctg cagaatacag agtagaagca
    25501 caccaatttt atgaacacat cggttatcat tccgataaaa tgcaaaaaag atttttgaaa
    25561 atcctgtaaa tctttatcaa aaaaataccc gatcaccaag ccgatacttg atgatcaggt
    25621 atttttgttt ttttgactaa gaaagtaatt gcatcaagaa accagagatg atcaaaacga
    25681 tggcaccacc aagtcgattg cccatttgag caaaagcaat aagttccatc cgattagctg
    25741 cagataagac ggctacattt cctgttccgc ccatcgagtt gttgcataag ccagcagtaa
    25801 tagttgattc aattggatat aaaccaaaca aacgaccgac aaatcctgaa acaactgaaa
    25861 tcgcgataac acttgtcaga cataagacaa caaattgcca tgtcaaagca gaagctaata
    25921 cattcagatt cagcaacgca ataccgatac cggcaagaac agctgctgtc aagtttttga
    25981 caatcaagtt attgaacatg atagctgcat cttcataata ctctggtaat aggttggcga
    26041 ttttacagat gacaacgata ataatcataa atgcgtaagc atggacttta ggaacaaaat
    26101 agttaccaat ttgtcctaag atgaagaaag tcaatgatac catcaaacca acacctaact
    26161 gtggaatgga aggtttcaca gttcgaggtt ttccatcgcc aagatcgcct ttttccatta
    26221 atttaccatt gccgttgaat tttggcattg attgtccagc acgagcaact aatgctgcac
    26281 cgatgatagc taagacattt gtcattgctg tcgcaggaat caggcgggaa atgatagaag
    26341 aagcatccgt acctaaaaca tttgcataaa tcgttgataa aggaacggct ccggctccaa
    26401 caccaccagc catcgcaggt aatgagatgt ataaaacgga atttgcaaaa ccatttccga
    26461 taagcatgcc cacgagtcct acggcaaaaa aggagacgac cattgaaata agtgcaacgg
    26521 gaataaaacg aacagaagct ttcatcaata gattacggtt cataccaagg atgctaccag
    26581 taatcaaggc agcaatataa aagtcccaaa agcccatatt gctgacaaag ttttctgtgg
    26641 atttcactac atattcaggt aagatcccaa atgtagcaat cgcagcagaa gcgaagatcg
    26701 caaaaacgga accaccaccc aaataacttt tgacgatagg gatttttgtc ccgataaaat
    26761 gaaataaatt accgaaaata accaaaactg cgattggtcc aatcatattc gtcggtaatt
    26821 tccctaaagc cattgcaatg atcaggacaa ggaccatgat caaataaagt ggcaaactta
    26881 cgccggtgat ttttgtggac caaaaacctt cttttttctt ttcctcaact gcagttgcag
    26941 ttgttgtttt ttgtgccatt tttacagatc ctttcttatt ctgtatcata tgaaaaaagc
    27001 caaagaaaag cgacaaaaga gcaggagttg gctggccgct gttttgccgc agtcgctttt
    27061 ttctttttag acgtcataca aagtttctgt atgttctttt actataacga tcttaatctt
    27121 ctaaagattt atattcagga acccatttag cagattcaac tgcttctttg atatccgtga
    27181 tttcttcacg attcaagttt tgtgcgacaa ctgattttgc gacagtttca gcaatcgttt
    27241 gagaaaattc agtgattttt gctactggag gcaagatagc tgctcctggt tttgttacat
    27301 caacgattcc gcctaatgct cgactagctt gagaaatgat ttcagcattt actcgtgtag
    27361 ctgttgaagc aatcaaacca agtcctaaac ctgggtacat caaagcatta tttgcttgac
    27421 cgatttggta agttacacca ttatattcaa catcagcagc agggatacct gttccaacta
    27481 atgcttttcc gtccgtccat tctattaaat cttttgcttt tgcttctgcc aattttgttg
    27541 gattagacaa aggcatgatg atcggacgtg gtgtatgagc agccatttct ttcacgattt
    27601 cttcagtgaa tgctccaggt tgggtagaag taccaatcat gattgtcgga tggatcgctt
    27661 ttacaactgc ttctaagttt gtcaattcat cagcattagt gaaatctgag cgtttgcgag
    27721 cgaatgggat ttgaccagga gtcaatcctt ctgtatcttc aaacaataaa ccttgtttat
    27781 ctacttgata gaaatgttgg cgtgcttctt cttcaggtac accttgacgg atcatctcat
    27841 ctaaaaggat gtttgcgata cccacaccag cagtaccagc accaaatgtc aaaattattt
    27901 gatctttcaa ttgttcaccg gatatattca atccgcctaa tacacctgct aggacaacaa
    27961 ttcctgtacc ttggatatcg tcgttgaaag tagtgatttg atcttcgtat ttttctaaga
    28021 tcgctgcagc atttcctcgt ccaaaatctt cccaatgcaa taacaattct gggaataatt
    28081 cggtagctga ttgaacgaat tgatcgatga attcatagta agtttcgcct tctacacgag
    28141 catgacgatt gcctaagtat aagggattat tcaataattc ttcattatta gtaccagcgt
    28201 cgatcgatac aggcaaaact tgtgctggat taataccagc agcagcagta tagaccatca
    28261 atttgccgat agcgatgtca acaccgttca caccccagtc gcccatacct aagattcctt
    28321 ctgcatcagt cacaacaatc aaacgaatgt ctcgtccatc tgccgcgttc ttcaaacttg
    28381 ctttgatgtt ttctggttcg tcgattgaaa gaaatgcagc atcttgcggt ttcaagaaaa
    28441 tttcgttgta ttgttcgatt gaatccgcaa cgacaggatc ataaacgaca ggcatgaatt
    28501 caactaaatg ttcgcccatc aatttataaa acaatgtccg attcgtattg aatagattca
    28561 ttaagaagat acgtttttcc aaatctgttt gtttgcttaa gtattgacca taagcttgaa
    28621 cagattgttg ttccagtgtt tggacagtac ttggaagcat accagtgatt ccatattttg
    28681 cccgttcttc ttttgtaaaa gctgttcctt tatttagaaa aggattattt aataagttta
    28741 accctgtaag catgaatgat tcctcctaag ttttaataca aacgaagtat agaagtcaga
    28801 tgatgatata gtcaaaaaga gcaggtatga taaattttat ttatatgtaa tatagccaaa
    28861 agaaatcaaa atagcagtat ttgttctttt tatatcgaca atccttgctt gaatactatg
    28921 cgtatctctt atatgctaga gaaagaaaag tagaagaata agggatagat ggaatggata
    28981 aataggattt ctgaggaaaa aagcacatga tagcgttgat aggagtcacc tatgtcaggt
    29041 gataaaaata cataagtaga aaattaagag gaggaagaga aaatgaaaga agttcaattg
    29101 attagaaaaa gcgaactatc agaaggcggt tgcaatgcat gtggcgtagt agaagcgaca
    29161 agctatacat taaaattaga ttccaatcaa gcaataatct ctgaactgac agtaggcggt
    29221 cttgtggaca gtctggcgct tgctgaaggt tttactggag aagatattta tgagatgttt
    29281 tcagaagtcc gtcagttaaa aaaaggagaa aaatgcatag aggttcatca tgagagcccg
    29341 aatgtgaggt tcaaacgtgg tgataatgag atgatcttca agaatcatgt gtctgatcac
    29401 actgaactat atgaaatcgt gaatcaaata ctgtctggga tttttgaatt aggaccttat
    29461 gagttcaagg aagaaaacgg taatccaaaa ttaaatgaag aatggcaaga aacgattgaa
    29521 acacaaagaa acaatccaca cttatttcaa tagtcttcca cagtatttcg taaaaatgtt
    29581 gatgaaaaag aaacaccgtg aatcagaggt cgtgaaaaac ctgccctgca tcaagtaata
    29641 agaacagcca aacaaaaata gctcctccta tttttcgttt ggttgggctt attaccgcag
    29701 atgcaagttt ttgaacaccg tttaaggaag agactgtgaa gtcaacgttc tgacctgagt
    29761 attagagaga caaatggcaa aatagttcct catattttgc cattttttga gctaataccg
    29821 agggtcagct gattgaacac cgttttatcg agaggttgtg aaaaagtcgc ttagtttcga
    29881 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttaata
    29941 ccgagaaaat ggcttttgaa caccgtttat tcaaaaaaag ggactttgac actacttttg
    30001 tcaaagtccc atcggttttt tatctatttt tattacaatc atttcttcca gagtagtgac
    30061 atctggaatg gctgaaatgt aaacagatta tttgcctgta tcaatgtcga agagtaatat
    30121 aaaaagtgta aacgctatca tattgttcat cattcaaatt ttatagagag aaggcattga
    30181 gatggaaaaa acatatcaag caggtacaaa tgaaggaata gtagatttta tcaatatgga
    30241 agatttagaa atcgcggctt cacaagtgat tccagctggt ggttatggtt acatcagcag
    30301 tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc atcgtttgat
    30361 tattcctcat gtattaagag atgtagaatt acccgataca actacacatt ttgacgaaga
    30421 aatgctgact gcaccaatca tcatggcacc agttgctgct catggattag ctcatgtgaa
    30481 ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc
    30541 ttacgcttct tgtactttag aagagataag agaagcaggc ggggagaaag caccgcaatg
    30601 gtttcaattt tatatgagca aagataatgg aatcaatttg gatattcttg aagtggccaa
    30661 gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga
    30721 aacagaccgc agaaacgggt ttaccttccc gctgccaatg ccgatcgttc aggcttatca
    30781 atcaggagta ggccaaacga tggatgctgt atataaatct tccaaacaaa aacttagtcc
    30841 aaaagatgta gaatttatcg ctgctcattc tgatttgcca gtctacgtga aaggtgttca
    30901 atcagaagaa gatgtctatc gatcattaga atctggtgct ggtggaattt gggtatctaa
    30961 ccatggtggc cgtcaattag atggggggcc tgctgcgttt gattcgttgc aatatgtggc
    31021 agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gtggacaaca
    31081 tgttttcaaa gcaatcgcat caggtgccga tcttgttgct attggcagac ccgttattta
    31141 tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga
    31201 attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa
    31261 attgcgtgag aatcgtttta tttaagaaat aaatgacgaa aaaaatagga gtgcggcaga
    31321 tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga
    31381 aaaaataaaa ttgaaatatt ttttcatact taataaaaaa ggaagaatat tcatgtaaac
    31441 gattttattg tttttttctc ttttatacaa aaagggtcat aatttcaagt aagcggatga
    31501 attcatgagg attttttgca tactgtctct actatactta ggtcactaag aaaaaggagt
    31561 gatttattat gaaaaagaac atgattggga cattattact ttcaacactt cttctaggcg
    31621 gcttggcaac accagctttt gctgagggtc aagcgacttc aaaaggagat attaccttta
    31681 ctgaaccaac aaataccgtt gagcctctta accctactga tccttcaaaa ccagtagagc
    31741 cagcagatcc ggaaaatcca gcaactggcc agacaggttc tttaacattg gatgtagttc
    31801 ctgaattgcc atttggtaca cacgagattg aaagcggaac aaaaacgtat caagtagacg
    31861 cttcaaaaaa cgatacacct tatctccaag tatcagatcg tcgaggtgta ggtgcagatg
    31921 gccaagcaca agggtggaac gtgacagttt cggtttcaga ttttgtaaac ggttcacaag
    31981 ttttgcaagg cgctgaatta gattttggaa catccactgt taaaagtacg tcagataacg
    32041 aatccactgg accaactagt caaactgtaa ctgggttgag taaagcaagt gcagcgacac
    32101 caatatttac agcagcaaaa gatcaaggtc tgggaacatg gctttctgtt tatgatccag
    32161 caaatatcac actgaaagtg ccgaaagcag cagctggaac attcacggct gatttgactt
    32221 ggaacctcgt ggctggccca gtagcataga gtatgaaata aattcaacaa actgcggtga
    32281 aaatactata ggtgaaggat tttttaccgc agttgttgtt gaaaggatgt ggtggacaat
    32341 gaatcaaagt aaacgactgt ttgtttcgtt tttttctatt ttaagtctat ttttcatttt
    32401 tccgtcaatc agccaagcaa aggattcagc aggagacttt ggaatcaaac cggtatttcc
    32461 agagaaccaa atagataaag caatcggtta ttttgattta ttagtggcac cggaacaaaa
    32521 tcagacacta gaagtaatta tcagcaattc ttcagatgaa gaacgaacat ttgaggtctc
    32581 tgtcaatccg gcggtaacga gtgatggtgg aacgatcgat tatagtcaaa aaaacccaac
    32641 gttagatgag acattgccgt ttgatgtccg tgacgttttg ctcattgcga aaaaggaaat
    32701 caatgtatca gcacatgcag aaacaactat acctatagaa gtgaaaatcc cagcaaaaag
    32761 ctttaaggga cgtgtattag cgggtattca tgtttcacct aaagaagaag ctgaaacaga
    32821 aaatgctaca gaaggagcac aaatcaaaaa tcgtattgct tataatctcg cagtcgtttt
    32881 acaagaaagt caagagacga tagaaccgga tctgaaactt ttatcaggag atttggacga
    32941 ggtcaacgca aagccaactg ttcagcttcg tttccaaaat cctcagccta ggattatcag
    33001 caatttgata tttacgagca agatctttta tgaaaatcag ctttatattg aaaatacgtc
    33061 taatgaattt ttagttgcac caaattcgaa ttttcacttg aatcttgatt tagctggtga
    33121 taaagcaaaa gccggcgatt accgcgcaga aatcatagca aaatcaggcg acagtaatga
    33181 atggcgattt acacaaaact ttacgatcaa aaaagaaaaa gcacaaaaag taaatgaaaa
    33241 ttccgttttt gcagtacaag aacaatcttt cccatggctt tatgcaggat tggttgctgc
    33301 agttctagtg ttatggttga ttttgctgtt tttgtgggtc cacaagaaaa agaaaaaagg
    33361 agaaacaaca tgatgaggtg atttcgttga aaacatcgat cttattggga gggattattg
    33421 caagtttgct gttcttcagc tggcaagagc cagtccaagc agataccaaa agttcaggta
    33481 cgattacatt ttatgatcta agtccagttc cgcatccaag tggttcagtc aatggcgcat
    33541 caaaacagca gacacagcta ccgatttata aagaatctaa tcaacatggc gtattcccgg
    33601 agacgaatga agaaaagatc cgttttcttt atttgatcat tggtatagaa ttatggcttc
    33661 ttgcgtggtt gttgttatgg agaagaaagg aaaaggagga tgaacatgtt gctacaaaat
    33721 aaattttcat tttttcgctc cttctttttt acttcgcttt ttgttttaag tatatttatc
    33781 ttttcacctt cggcagaagc tgttggagca acgcctcctg ccatttctta tgatccatac
    33841 acgtatgaaa atatcgtacc tccaaatggt ggacgtagtg cagtcaatgg cgggaagctt
    33901 gtccaactaa ctggggctaa ccaattgcct aaattgactg ctacttattt gaacgatggt
    33961 tcaaatgatc cttggaaagg tggcacggtc cgttttcatt gggaaagcca tgtgaaacta
    34021 acaaatgtac ttgatttggt gaatatcact gcacaaggat ttgcatttgt ggcgatcatc
    34081 aatttgccgg aacatgtatc aagcagtgat gtattaaaag caattgactg gaacaacgcc
    34141 tacttgtata ttgccggtgc ccccatcaag gttactagcg gtagtttatt atcagcagga
    34201 agccatacct tgcgttttgg gatggggtcg tgggatacaa atactctatt ggcagatatt
    34261 attgctatta ttacgcgagg cgaattcaac gtggacgata tcccgcttca atttgatgtt
    34321 acagctaatg tcgcgaagat gacagaaaac ggagataaat atgatacgag tccaaacaaa
    34381 attttgacca agggtaaatt acaaccttct atgtctaaaa aggccgtttt ttctgttgat
    34441 ttttatgatt cgaataatat tatccaagat cgtatgttca attcgatttt agctccagct
    34501 gcaggtagat tttatcctaa attaactggc ggaaacttga atccaggagc atatgcattt
    34561 tctgcaagtg cagagataaa tacgtggaat agttacatct caacaagtga ttcaacagga
    34621 aaatataata catttgctga taccacagaa tacgtggatg gaaataacca gaaccttcca
    34681 ggctctacca atagtcggac attgacgatg gggctgactg aaggacgttt tgatcagttg
    34741 ccatttagtc gctttgatcg cgtagtcaac tattttacag gaaaaaacgt tacacaagga
    34801 gcaatactga ctcatactcc agcaagcaca agtggattga atcagacagc caatatcgtg
    34861 tacagtggga aagatgcgca aggcggattg ctttctcctg ttgctttaaa ggtattggag
    34921 cagtacaagc tagacggaac ggttttacca acaaacttaa cagccgatag tcagtgggga
    34981 aagctccttt cttctgcatc gtaccaagtc acggccaatt ggtcagcaac tgaactaaaa
    35041 aagggttcga tccattacag gctttattcg aaaggaaccc aaaaattagc gacaggatgg
    35101 acagatcaac ttttccaaac gatccaatca aataacgggg gtgtaaatac agctacttct
    35161 acactacctg cattggcttc agggcaatat tattttgatt atcgcttggt ggacgatgta
    35221 cttactaaca tgtatccgtc ttttgcgtac aaatggcagt ctgaacaagc tggaatcacc
    35281 actcttcctc aaataacagt ggctaatttt ccatctgtga gtgcagatag tcagctaaaa
    35341 aaccttagca gacaacctga gacaggagat cccctgtctg ctctatcagg agatacgatt
    35401 caagaaaatg tgacattcac gttaacaaaa tctggggatg cgataaccga caaaaaaatc
    35461 acgatctcac ttcctgaaaa tactacttat atcaatggtt ccttaaaatt aaatggtaca
    35521 actatcgcag ataccggcat tcaacaagga gtttctgtcc cagcagatct attgagcaaa
    35581 ataggagata ccattcatct tacttacaac tatcaattaa atactgtaga tacttctgta
    35641 caatccgtca gcatactgac aaaggcagct gtactttcca gcaatattac attagcagat
    35701 ggtgctaagc ttcctaaccc agttgtccaa acatctgcta aaacaattct tgtccccaaa
    35761 caagaattaa cattagttaa tgtaccagat gattttacat ttggtaacga tctccccaaa
    35821 ccgttgaaga caagttatta tgaagcaaaa ggcgattttt cttttgatgt gcgggatact
    35881 cggcttccaa gcacgtctcc ttggcaactg acagggacat tgacttcttt atttaagaat
    35941 gatcaaggtc aagaattaag cggaacaaaa ttatacttca atcattctgg atcaaaacaa
    36001 ttgattcagc aaggtcaaaa cacgttgatt tatgagagtg atggaacagc taaaggagag
    36061 gttctcgttg actttcccga taccgatggt ttactcttag aggtaaatag cagtacgaat
    36121 gctcaacctg gcgcaacgta tcaaggcatg gtcacatggg aactgacagc tgggccaacc
    36181 agctaagtga ttagaatttg tatggatctg tgataactgt cacagatcct ctttttcaag
    36241 gatgagaagg aaacttctgc tgttaaaatc agaaatgctt ggaggagtgg acttggaact
    36301 tttcagtttg cttgaaaaaa atgaacaacg gcaaatcaaa cttttccaac ttttattaga
    36361 ggaaaaagga gagatttccg ttaataacgt acttacccgc ttatcagttg atcgggctac
    36421 cttgaaagaa gatctgtttt atttacaagc taacttggaa aatatcaaac aattggaaat
    36481 tgttatccaa gaagggaata ttttattaat cagacatgga aatatagcag cagtcgatgt
    36541 ttattatcat tgctacttgc gctattctac aaaatatcag atacttcttt atctgttgga
    36601 aaatggctca tttgatcggc agaaacttgt catggaactt aatatcagtt cggctacttt
    36661 gtctcgcaga gtgaaggaat taaatgattc tttaaatgag tttcatctga aaataaaaag
    36721 cgggcggctg accggtccag aaagtcaaat ttgttatttt tatttccaat tattttggtt
    36781 tggacgtcct tatcttgaaa acaaaaaaga atacagtgat ttatcaggaa acactttttt
    36841 agatctgctg caaaaagagt ttagttttac tattacagaa gaaggacgat tgaaatttga
    36901 aatatggctt tccattatga agaagcgaat gcgctgtacg aaaaaatcca aaccagatta
    36961 tagcaatctt cctgttggat tgtttcaaac gacacaattt ttattggacc tccatacatt
    37021 tttgacccgt tacttctttc aatatgcctt tccttggaat caaaatgaaa cgttggtttt
    37081 ttatcttttc atgatcagta actttacatt agatacacat aactcgcatg tccaggagtt
    37141 tttgataagc gaaaaataca aagacgacgt agtagctcaa ttgaatcgaa cgtttaaaca
    37201 atcgatagaa gaatatttct ctttttatgt ttttccagaa gagatttcgc aaaagattgc
    37261 gttgacagtg atgcaaaatc acgctcattt tgcctatttt agaggatgga tgaatatttt
    37321 tggccaagaa ggattaaaac agcggttaac tgaagtagaa gataaacaat tcctggtttt
    37381 atgtgaaaga ttggtcagac aaacatttgc tattgttgat tttactgaag tagacgagga
    37441 aaatgcccgc tttgaattat ttgctcgcta tgcatctatt ttgcggacgc tgatcaagca
    37501 gttagaatta cgtctgcaaa ttgcttatga tcttccttat gaaaggctaa tggcggatat
    37561 cgtgaaagaa atcatccagg aacaattgct gaatacgatc aaatatgatt tagtcgaata
    37621 cgagaaagaa aagcagtatg atatcatttt gactagccaa ttgaaagagt acccgtcaca
    37681 gaagtccgct aaagtttttg tattcactag caacgaatat aattatgatt gcccctattt
    37741 gaaccaattt ttaaaagaat gttatttaga aaaattgaat ttaaggatga ataaaacatg
    37801 aaaatcgtga tcattggtgc ttcccatgca ggcatcacag ctgccctaaa tctaagaaaa
    37861 cttcaaccga aagcagaagt tttactgatc gatgaagatc ataaagatgg actaggctat
    37921 gtttcaaatg gaatcaacct ttatttaaaa ggaaagatac gtagtttagc tgaagttgcc
    37981 cataacatgc ggactcttca agaaagtggg gcaaaactga tcaccgaatg gagagtaact
    38041 gaacttgatc cagacaaaca tcagctcatc ctcgcatcca aagaaggaaa aaacgagacc
    38101 ataacctatg ataaattaat cgtagcaaca ggttcctctc ctgtcacctt gtataaacaa
    38161 attgaagctg aaaatgtcta cacctataaa aatctagtgc aatctaagca agtacttgcc
    38221 gctttaaaag aagcaaaaga agtagtgatc ttcggtgctg gttatattgg attggaatta
    38281 gcagatgctt tgagaaataa aggatacatt attcatctag ttgattatat gccgaatgtt
    38341 ttatcaagat atttcgataa ggatatgatc aacagttttc aaaatcagct gcaaactaag
    38401 caaatcaatt tttatccaaa tgaattcttg atcgattgga aaaaatcaga agagaaagta
    38461 gtatccgtac aacttctatc tcaagcaata aaggcagata tggtcatttt ttctgcccaa
    38521 actcgtccta atacgacatt gttaaaagaa aaagtggctt tgtatgagga tgagactgtg
    38581 atagtcaatg aatatctgca gacaagtgat ccggacatct atgcgattgg agatatcgtc
    38641 ccagtcagtt ttgacaaaaa taaacggcat ctctttttac ctttagttac tcgagctgta
    38701 catatggcaa gagcagtagc gctaactttg agtggtcagc caaccgctta cgacctcaga
    38761 caaaagataa cagcagcagt cattatagac tactttttag gaactgtagg gctgacagaa
    38821 gatgaagcgc cattcttgga acaaaatacc ggttcgtgtt caggagaatt tgatctattt
    38881 ccacaatacg atgaagaaaa caaaacagta aatgcaaagc tgatctacca tccggataca
    38941 ttagaaataa ttggcggaca gctgataagc caagaattct tattatcaga tttgaacctg
    39001 cttgcggata tcgtgaaaca caaaactacg attccacagc tagcagttga ggattttgga
    39061 tttctgtctg aacacacctc tcgttttcac tacctcaatg agttagcttt taaagtacta
    39121 gctaagagag caaatagcaa tagagtagat aaaagactgt gagaaatatc acggtctttt
    39181 attgtgtggg gatataataa atattaatca atcgagaata aaaaattcat ataatgaaat
    39241 agcggaagaa ttttttgagc ataatattcg ccaaaaattt atcaagtgat aaaattgttg
    39301 tataaaatga ttatttcagg aggaggagtt aaatgttttt agcattaaat gaaattatgc
    39361 attcaaagtt acgttatgcg ttagttgcag gggtgatgtt tttgatcgcc tacctggtat
    39421 tttttttgac tgggttagct tatggcttag ctcaagataa tcgaacagca gtcgacaagt
    39481 gggaggcaga ttcgattgtg ctttcgaaag atgcaaattc aaatctaggg atgtccatga
    39541 ttactaagaa gatagcagaa gaagtcgaag gtgggaaagt ggcgtatctt gcccagacac
    39601 ctggagtcgt gactagtaaa gacagtacag aagaaggaaa aatcaatgtt agcttcttcg
    39661 gtatcgataa aaatcaattc atcatgccca atcttgttga aggaaaagct tttgataacg
    39721 atgatgaggc agtaggcgat attagtttga aagaagaata tggtctagcc gtaggagata
    39781 cagttaagtt atctggtagt gacaagacgt ttaaactaac tggctttacg gatcatgcca
    39841 aattcaatgt ctcaccagtt ctctatacaa ccatcaatgc ttaccaagag atccgcttcg
    39901 aaaaagaaga tacaagtgaa aatgcacgga ttaacgcaat agttgtcaga ggtaaaatca
    39961 gtaatttacc tgaagattta gaacagatca aaatttctaa attcatcaat gaattacctg
    40021 gatataatgc gcaagtattg acttttggat ttatgatcgg atttttgatc gttattgcgg
    40081 ctattgtgat cgggattttc atttatgtac tgacgatgca aaagattaat atctttggtg
    40141 taatgaaagc acaagggatc acaggaggat ttattgcgcg ttctgtagtg gcacaaacat
    40201 tcattttatc atttgttggt attcttcttg gtctattagg aacagtggga acatcccttg
    40261 ttctacctga tgcggttcca ttccaaagca attggctgtt ttttggcgtc attagcttgt
    40321 tgatgctggt agtagctgtg ttaggcgcac tattttctgt tcggacaatc gtaaaaatcg
    40381 atccgttgaa ggcaattgga taagaaggag gaagagcatg aaagcaatag aatttaaaga
    40441 agtagaaaaa agtttccaag atggcgacca agtagttaaa gcattgaaag aaacaaccat
    40501 gagtatcgat aaaggggaat ttgttgcaat catcggtcct tctggttcag gtaaaagtac
    40561 ttttttgact ttggcaggcg gattgcagac gcctagcagt ggctctgtat ttatcaatga
    40621 tcaagaattc agtaatcaaa aagaaagtga acgcgttaaa ctacgtttta aagaaattgg
    40681 ctttattttg caagcttcca atctggttcc ttttctgaca gtaaaaaagc agttattatt
    40741 agtagataag atcaaacatg aacatcgtca aaaagaagca aaagaattat ttgagcagct
    40801 tggcgtggat aagttgatca acaagtatcc agaagaacta tctggtggag aacggcaaag
    40861 ggtcgcaatc gctcgtgcat tgtacaatga tccgtccatt attctagcag atgaaccaac
    40921 tgctagtttg gattcagaaa aggcacgaga agtagtcgat atcttggcaa aagaatcaaa
    40981 agacaaaaat aaagcaacga tcatggttac tcacgataca cggctgattg acaaatgcga
    41041 tagaatattt atgatggaag atggtgtatt gaaagaaaaa gaataaccaa aaagcaggtg
    41101 gaaaaatggt ccatcgatac aacaaagata cacagcaaaa aattttgaaa gaagcgactg
    41161 acttgtttat gtccaaggga tatctcggca cttcaacaag agagatcgca caaaaagttg
    41221 ggatcaccca acccaatctc tatcattatt tcggtgataa agaaaaacta tatacggcag
    41281 ttttagaaaa ccacttaaaa gatgttggaa aagctttgag agaaatcgtt caaacaagcg
    41341 aaacgggatt tcaagtaacc ttgacaaaaa tggcgcagtt tttgattgag acacatctgg
    41401 tcgatttgtt tctgatgctt cacgatcttg aaagcaatct ttcaaaggaa acaagagatc
    41461 atcttttctt tctatggaag aaaaattatc gtgaaccttt tgaagaaatc ttttcagcaa
    41521 accaatctgt gctaagaaat gggatttcaa aagaaatcgc agcaaggcat tttttcttag
    41581 tgttagcacc ttacatcact cggtcgaaaa gacctgtgga agaaccgcta aatgtggcgc
    41641 aattgatcga tttatatttg aatggtgtat taggagaagc gtaaagctgt tatataggga
    41701 gaaaaatagt taggtgaaga agcaagatat gacgaagatc gttatatctt gtttttttta
    41761 tataggtagt ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt
    41821 ttatttatta tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt
    41881 ttgtagaatg aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag
    41941 atttcaggat atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt
    42001 tatatctttt acagcccctt ttttgcgtac ttaagctgat tttaagtttt gacccttagt
    42061 ctggatctac gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa
    42121 tcattatcat tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac
    42181 aaacggtcag cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag
    42241 aacaagagac attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc
    42301 taactacccc acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa
    42361 aaaatgctta tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg
    42421 gaaccaaagt cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg
    42481 tattagatcc tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag
    42541 aagaaaaaga tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg
    42601 gagcaacggt ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg
    42661 actacagtga agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat
    42721 atgcgaatga agcaactgga atcacgacat attattacta tggacaagag gaaacattag
    42781 cacagacaat tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg
    42841 ggaactatca agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata
    42901 tgaataatga tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg
    42961 caagtttgac acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa
    43021 aatagctcct catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttgaac
    43081 accgtttaag gaagaggttg tgagcctgcc gtttaactcc gcgtcacaag gagcaatttc
    43141 aaaaaacagc ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag
    43201 aacaccgttt attcggaaca agggactgtg acatgacttt tgtcacagtt ccattatttt
    43261 tttagcagct tcaaagcttt ttctgtattg gcaaaatgaa gtttggcata tttttcaagc
    43321 ataggcatgc cgcctttctc caatagtttt gccgctactt ggtcagattt cgttccagct
    43381 cctgatggaa gggtgattcc agcttcagct gatgctttgt gtaactcttc caaaaagctg
    43441 gcacgactga tgatagaagc agcagcaaca gctaaatgat attgttctcc tttagtaacg
    43501 aagtacagtt tttcttgtaa ttgatttttt tctaaacgcg catatttttt aaaatttgct
    43561 tccggcgtaa actgatcgat caaaatcgct tctggttttt ctggctcgat tttttttagc
    43621 agcaaatgga tcgcttgatt gtgcaaagcg actttcatcc gaacggcatt gtattttggt
    43681 tggatctcat tgtatttttc tggagataag acaagcagtt tataggggat ggtttttttt
    43741 aagatgtcag caagctggat gatttggcta tcttttaact cttttgaatc tctaacacca
    43801 agtttacgta attctgcaag ctgatcacgt ttcacataag cagcacatac tgttagcgga
    43861 ccaaaatagc tgccatttcc gacctcatca ctacctaaaa cggataattg actgatattt
    43921 tttggcaaag aagaggctgt ttttgctggc ttggcttgtt tatcctgtgc ctccccccat
    43981 ttatcagctt ctattgctgc attcgtacct tgaaacatca cttttccgga tgtataagcg
    44041 gtaatggtcg tcccgtcttt ttttgcagca aagctagtat aaggaatctg tttatttaac
    44101 aaataagagc gatagtatgc tttcatttga ctaagttggt cgtttgttgc tttgataatg
    44161 acgttgctca taaaattctc ctcgttttta tacataaaac tataccgcaa ctcacgactt
    44221 ttcgttttct aagagattgc gagacatagc atttttttaa agtttatcat ataatagtta
    44281 agtatgtgaa aaatatttgt ggagaacggt gaaagaacca acttcatggt acactaaata
    44341 aaactttcat caaataggag gaaatgattc gtatggcgca cgaaaaaaca cgctacaaag
    44401 cagtgattgc caaccaaaca tatactatta tcggacggga aaccaagcat catatggata
    44461 tcgtaacgaa gctgatcaat gaacagttag cagaattgaa acaattatca ccacaaatgg
    44521 ataatgaaca agcagcaatc ttgatggctg tcaatgcgct ttcggatcaa ttgaagaaac
    44581 aagaaagaat cttggagctg gaagaagaaa cagcagaatt aaagaaaaaa atgatcaaat
    44641 ttacagagct tgaaaatcgt gtgaaacgca ttgaagcaat cgagaacgaa gctcgggaag
    44701 tattgaaaga aaatggtcaa gcagatcata ccattaaaaa tcatgtggaa gcacaacaga
    44761 ttttaaacga gaaacgaaaa ggacaaatta aacaaaaatc ttctaattaa gatcctttta
    44821 tcaaaataag aaaaatttaa aaaagttaga agagaaagga acgtagcatg ctgagtttgc
    44881 tgattttatt tttattgcta attgcgtttt tttctggagc acgtcgcggg tttgctttgc
    44941 aaatcatatt cgcgattgga tatgtattgt catttattgc tgcccagcat ttttacaaac
    45001 cattagcaag tcatttggaa ttatacattc catacccagc tgtcacccca acatcaaagt
    45061 tagcattttt tgatcaggta tttgcttttc acctagatga agctttttat gcaggaactg
    45121 cttttctgat tattttgcta attggctggc tgcttacccg atttgtgggg gtttttgtcc
    45181 atggattgac gtatgtgccg atcttgagac aggtagactg gattgcagga ggcatcctgt
    45241 cactaatcat ggcttacgtt actatatttt tgattttgca gttacttgcc tttgttccat
    45301 tagatgtggt ccaaaatcaa ttcggcgagc atagtttcgc tcgttttatc gttgagagaa
    45361 cgccatttct taccaacaag atgtatgagc tttggtaacg aatattttga attaaggaaa
    45421 atagtgctgg aaaatatttc gttttttgta cagagtagtt tatttaagaa ggctgcgtag
    45481 cgtatgtccg gcacagctct tttttcatag tgaaagataa ggtgataaaa tgaataaccg
    45541 aatccttgaa acattggaat ttgaaaaagt caaacagatg gtcagacaat ttgtagtgac
    45601 tgcacaagga aaagaagaac tggcagaatt ggtaccagtg agtgagaaac aaacgatcac
    45661 aaactggctt caagaaactg aggatggatt gaaagtgcaa cgtctgcgag gcgggatccc
    45721 tatacctaaa cttgaaaata tccgtcccca tatgaaacgg atcgagatcg gagcagactt
    45781 gaatggagtg gagcttgcac aagtttccag agtattgtct acgacaagcg aattgaatcg
    45841 tttcattgat gatctttctg acagtgaaat cgaatttgca cgtttgtata tgtgggcaga
    45901 tcagttagtc acgatccctg ttttgagccg ccgtttaaag gaagcaatcg atgaagacgg
    45961 acgcgtgact gatgacgctt ctcctgagtt aaagagcata agacagaaca ttcggcgaag
    46021 tgaacaagct gttcgtgaac agttagatgg gatcgtccgt gggaaaaatg caaagtattt
    46081 aagtgatgcg atcatcacca tgcgaaatga ccgctacgtt atcccagtca aacaagaata
    46141 ccgcggtgta tttggcggtg tagtccacga tcaaagtgct tcaggacaga ctttatttat
    46201 tgaaccaaaa caagtggtgg atttgaataa tcgtttgcgt caataccaaa tcgctgaacg
    46261 aaatgagatt caacgtattt taagtgaact ttctgctgaa cttgttcccc atcgtcaaga
    46321 aatcatccac aatgcttatg tgattggtaa aatggatctg atgaatgcaa aagcacgatt
    46381 tggtaaagaa gtaaaagcca tcgtgccggg aatcagtgaa gacaatcatg tcgtcttgaa
    46441 acaagcaaga catccactga tcgatcagga aaaagtagtt tctaatgaca tcacaatcgg
    46501 aaaagactat caagccatcg tcattacagg acctaacaca ggtgggaaaa cgatcacgtt
    46561 gaagacttta ggtctgttac aactgatggg gcaagcagga ttaccgattc ctgcaggtga
    46621 agaaagtcaa atcggtattt ttgaggaagt atttgcggat ataggagacg aacagtcgat
    46681 tgagcaaagt ctatcgacct tttcttctca tatgacgaac actgtagata ttttatcaaa
    46741 agtcaatgaa aaaagtctgg tattatttga cgagctcggt gcaggaacag atccgcaaga
    46801 aggtgctgct ctagcaatcg caatcttaga tgatcttggt aaaaagtctg cttatgtgat
    46861 ggcaacaacg cattatcctg aattgaaagt ttatggatac aatcgggcaa atacgattaa
    46921 tgccagcatg gagttcgatg tggatacact aagtccaacg tatcgtttgc tgattggcgt
    46981 tcctggacga agcaatgcgt ttgaaatctc tagccgatta ggtcttgata cagaagttat
    47041 tgatgaagcg aagcagttga tgaatgatga gagccaagat ttaaacgaaa tgatcacaga
    47101 tttagagaat cgccggaaaa tggcagagac cgaatatctg gaaatgcgtc attttgtttc
    47161 tgaggcacaa gaacttcacg atgatttgaa agaagcttac agctatttct ttgaagaacg
    47221 tgaaaaagaa atggaaaaag caaagaaaaa agcaaacgaa gtcgtgtcag aggcggaaga
    47281 aaaagcagaa aaaatcattg ctgatattcg taaaatgcaa cagcagattg ggcaaggaaa
    47341 tgtgaaagag catcagttga tcgatgcaaa gacccaatta gcaaatctac atcaagaaga
    47401 aactttgaag aaaaataaag tattgaagaa ggcgaaagaa caaaaaacgt tgaaaccggg
    47461 agacgaagtg ttggttacga cttacggcca aagagggacg cttttacgta aaaacgggaa
    47521 tcagtggcaa gtagaaatcg gtatattaaa aatgaatgtg tccgaagacg agctgacgcc
    47581 tgttgctcct caaaaagagc cgacacaacg agttatccat gctgtacgat ctgaatctag
    47641 tagtcacgtg ccgaatcaat tggaccttcg aggaaaacga tacgaagaag cgctaagtga
    47701 ggtagatcaa tatttggatt ctgccattct agctggttat cctcaagtga ctattgtcca
    47761 cggaaaagga acaggcgcac ttcgcaaggg aattactgat tatcttaaaa accatcggag
    47821 cgtcaaaagc tttgagtttg caccggcaaa ccaaggcgga aatggtgcga caattgttaa
    47881 atttaagtaa agcttactaa aggtaagcag atggttcgct atccaagtat agaaatgcta
    47941 tactaatcat agattttaaa aatggaggtc tttgatatgt cacaagtaat tacagataag
    48001 acatttaatg aagaaacaga taaaggactt gtattgattg atttttgggc aacttggtgt
    48061 ggtccatgtc gtatgcaagc accgattttg gatcagttgg aacaagaata tgatgaagaa
    48121 gaattcagaa ttgctaaaat ggatgtggat gaaaatccag aaactcctca acaattcggt
    48181 atcatgagca ttccaacatt aatgttgaaa aaagatggtc aagtagtaga aaaagctgtt
    48241 ggtgttcatt caaaagaaca actacgtcaa atgatcgatc aatatttata aaaacttaca
    48301 gcaaaaataa gctgagggtg ggactgaatc cagttccgtc ctcggttttt ttgcggttga
    48361 gttaaggtta ttcaaattaa tattttttga gtataattac gttgtcaggc tggattgttt
    48421 ttttgacaga catgctataa taactttaat tgaatatgaa ggggagtacc agatgaacga
    48481 acgaatcaaa aacaaattag ctttacttcc tgatcagccc ggctgttact tgatgaaaga
    48541 taaaaatgga acgatcattt atgtcggcaa agccaaaata ttaaaaaatc gtgtccgctc
    48601 atattttact ggcagccata atacgaaaac ggaacggctt gttagtgaaa tcgaagactt
    48661 tgaatacatc gttacagaat cgaatatcga agcattgctc ttagagatca atctgataaa
    48721 gaaaaatgat cctaaataca acattatgtt aaaggatgat aaaacctatc ctttccttaa
    48781 gataacgaat gaaaaatatc cccgtttggt gattactcgt aaagtactaa aagacaaagc
    48841 actgtatttt ggaccatatc ccgatgtcgg tgcggcgaat gagacaaaga agatcctcga
    48901 tcgattattt cctctgagaa aatgcaaacc tagtcagacg aaggagcctt gtctttacta
    48961 tcatttggga caatgtctgt gtccttatta tttcgacgta gatccgaaag tctatacgaa
    49021 aatcgttgat gaagtgaaaa agtttttaaa cggaggacac gaaaaaatcg agtcagagat
    49081 ccatcagaaa atggaaaaag ctgctgaaaa catggagttt gaaaaagctg cagaataccg
    49141 agaccagatc cgtgccattg agacaatcat gactcgccaa aaaatgacaa atactgattt
    49201 actggatcga gatgtatttg gttatgcgat tgacaaagga tggatgtgtg tccaagtgtt
    49261 tttcgtaaga caaggaaagc taattgaacg agatgtttca atgtttcctt tctataacga
    49321 agcagaagaa gatttcttga cttacctcgg tcagttttat caggaaaatg agcatttcat
    49381 tcctaaagaa gtcttgattc ctgatacgat cgacaagccc agtgtagaag ctttgttagc
    49441 tacgaaagtg ctgcaaccaa aacgaggaga aaagaaaaaa ttagttgaat tggcgaataa
    49501 gaatgcctca gttgctttga atgaacggtt tgatctgatt gccagaaaac aggaacggac
    49561 gatcggagca gttgaacgtt taggaaacgc gatgaatatc ccgaccccaa tacgtatcga
    49621 gtcttttgat aactctaata tcatggggac gaatcctgtg gctgcgatgg tcgtattcat
    49681 tgatggaaaa ccggcaagaa aagaatatcg gaaatataaa ataaaaacag tcaacggacc
    49741 ggatgattat gcatcaatga gagaagtcat ctatcgcaga tattcacgag tgattcgcga
    49801 agggttgcct ttacctgatt tgatcttgat cgatggagga aaaggacaag tagatgtggc
    49861 aaaagatgtc ttagaaaatc aattaggaat cgatattcct attgctggca tggcaaaaaa
    49921 cgataaacat aaaacaaatg agttgctatt cggtatagat ttggctgtag taccgcttga
    49981 gcgaaattca ccagagtttt tcttactcca gcgaatccaa gatgaagtcc atcgattcgc
    50041 aatcactttc catcggcagc tacgcagtaa aaacagtttt gcttcccgat tagatgaaat
    50101 tgaagggtta ggaccaaaac gaaaaaaaat gctgcttaaa gagttcaaat cgttgaaaaa
    50161 tatcacagca gccacgacag aagaattaca agaaatcgga ctgccgaaaa atgtcgcaca
    50221 aaacgtattt gataaattac atcaggaatg aggaaagggc actttacaaa gaaagtaaag
    50281 tgtcctatca ttttatgtac aacaaaaaga gcgtttacgt tttgttggta aataataaat
    50341 cgcttacgtc tggtgtctaa ggatatatgt gatttcttga agaaggagtg aagaaatgtt
    50401 agaagtcaag gatcttgtta aaacttttgg gaactacaca gctgtcgatc atgtctcttt
    50461 ccaaatcccc gatggaaaaa tcatggggct aattggccaa aacggggctg ggaagacaac
    50521 gacttttcgt ttgatcttgg attttttgac acaagaccaa ggagaagtgt tatggaatgg
    50581 ccatcatctt ggggagaaag attatgatgt catcggttat cttccggagg aacggggact
    50641 ttatccgaaa gtaacgattc aagaccaact gctttatttt gcagaactta gagggaaatc
    50701 aagaaaagag attgaaccaa aaatcgattt ttggatggaa aaatttcagg taaaagggaa
    50761 aaagacggat aaagtaaaat ctttgtctaa aggaaatcag caaaaagtcc agctgatcgc
    50821 cacgttaatc catgaaccta aattgatcat tttggatgag ccattcagcg ggttagatcc
    50881 ggtaaatgct gaattgctaa aagatggaat attggaattg aaaaaacatg gttcatgtgt
    50941 cattttttct agccacaata tggataatgt agaaaaaatc tgcgatcatt tgatcatgtt
    51001 gcgaaacgga aaaatggttt tgaatggaaa agtacatgag attcgtgaat catttggccg
    51061 tacgaagtta ttcttggaat ctggtttaag ccaacaggaa gtagaagaaa tcgaaggcgt
    51121 gaaaaaagtg atccttcatg aagacaatac gttagagatt accttagaag atcctgaagc
    51181 tgggaaagaa atctttactc gtgcgactca gttcggctac attcctatgt ttaaccagca
    51241 accgccaact ttggaagaga ttttcaaatt gaaagcaggt gaaatcgatg cgtaagtttt
    51301 ggattattac caaagatgtc tatttaaaaa atgttaagtc gatttctttc ttgatcatga
    51361 tattagtccc ttttatcatg ctgggtatca tttatgtagc aggaaacttt gctcaacaaa
    51421 atagtgatgt caataaaatc ggcgtattgg ctgataatga gcaaatcagt cagcaaatga
    51481 gccaagtcaa aacagatgat tttgagttca aagcagttga ttctgaagaa aaagcacaaa
    51541 aagagctttc ggaagaaaaa atcgatgcgt atttagtagt ctcaactgag aatgataaga
    51601 taaatgggat gctttatagc gaaaattcat taggacaaag tacacaacta ctgatacaac
    51661 aacaactgac tggcttgcaa tcgatgcttc gagcaagtca attaggcatt tcaccagaag
    51721 aagtagctag cttgagccaa caagcaagtt tgactcgaca aaaagtcaat tttgattcaa
    51781 aaggaaaaat gacagttgga gaagataact cgacagtcca atatgtcgta agttatgtag
    51841 caacgattgt cttgttcatt attattttga cgtatgcgca gatcattgca caagagatcg
    51901 cttctgaaaa aggcacgcgg atcatggaag tgatcctgtc cagtacgact gcacaaaagc
    51961 atttttacgg aaaactaaca ggtgttttac ttgttgcatt aacgcaaatg gcattatacg
    52021 gtattatttt tggtatagga tacagtcaat tcaaagatat ggaaatggtc aaaaatatat
    52081 tagacggtat ctcattggat aacttgattg gaccattctt gtggtattct cttctattca
    52141 tgatctttgg cattttgatt ttttctgttt tagcagcttt atgtggctct ttggtcaaca
    52201 aagcagaaga tacagcaaaa gcaattttgc cagtgactta tctatctcta ggcggatata
    52261 tgcttggatt gatactagga gcttcagatc caaacaatat cgttattcgg atcacttcgt
    52321 atattccatt cttatcttca tatattatgc cggttcgctt agcaaatgag acagtcggta
    52381 tgggtggagc attgatttcc ttgttggtac ttgtagccgt aacattctta ttgatgatct
    52441 tgtctgcaaa catgtacaaa tcgaatgtac tcgtgtatag cgaaggcgga ttatggagtt
    52501 cattgaaaca atcgatttca attatgagaa atgaacgaaa aaaagcataa aatatagaaa
    52561 aatggctctt tgtcaaatga tgttggtaaa gagaaatctt agaataattg gagatcaagt
    52621 ggatcagttc ctcttgatct tttttagttg aaaatcaatg cgttgccatg acgaatcatg
    52681 atgcgcaatt tgaaattata aaagctacga aagccatatg catttcgttt taacactttg
    52741 atatggttgt tcagacattc aagtgggcca tttgaataac tttgttgtaa agcatttttg
    52801 atttgtggca gaaatttctg gaacgttctc aaggtcgtct gatagccttc gggaagcatg
    52861 atattattgg taagtagttc ttcaaacgat acgtagtctc gttcctttac agcgtaaaga
    52921 aagtcttgat ataactgata gcctacacgc aaagcagggc tccctttcag taagcgatcc
    52981 acaatatcgg tctctgttaa atgagctttg aagccaggtc gccaaaggcg tcgagtataa
    53041 tcaagcttgc gttcatcttt ttgaagaagt ttccagtatc tttttaattg cttacctaat
    53101 tgatagtgtt tttgttcttt gctttttaat agttggttcg tttctttgat tcgatgattg
    53161 cgaaaggtac gaccaatatg ttggacgatg tgaaaccggt caatgatcag ctgtgctttt
    53221 ggaaacagct ttttaacgag cgatacataa ggagcgtaca tatcaatcac gatccattcg
    53281 accgcttcac gaatttctcg tgaaaaatga ctaaaataac gttttaaaaa aggcagttgt
    53341 cggttctcga caatgtccaa caagcgctgt gtttgtccat ccatcatgat gaaactcata
    53401 gcacctgaaa caccccgaac tgatttgaat tcatcaaaac aaagtacctt aggtaacggt
    53461 gtccgaaaag gattcaacgg acgataaaat cgcttcataa tacggtaaac agaagagacc
    53521 gatagatttt tctgagcggc gatctctgtc attgaggtat tttttttcag acgagtcacg
    53581 acagaccagc ggactttctc actgatgaaa cagtgttttt tcgcaacaga agtatctgcg
    53641 acaaaggtcc gctgacaagc tttgcatttg aagcgttgct ttttcaattc gaggtaggta
    53701 cggcattcag aaacatcgtt cattaacaac cggacggttg ttgtacccca acgcacaata
    53761 tctgaagcaa tctggtgttt acagtggatg cattcttggg ctgtataaat caagtttcca
    53821 cgataaatat gacagatttg ccctttgtat ttttcttttg tgagacaatt ttcgttaaaa
    53881 gtaactgata agtctagaat atctaatgtt tctttgataa ggtgagtgta agacatcttg
    53941 attcctcctt ttgggttggt tcgccaaatt aattctaaag gatttcaagg tgtcttttct
    54001 atatgttttg gaaaataaaa aagatgctgg tgtcatccac caacatcaaa aagtatagag
    54061 ctagaaaaat atagaaggaa gctggacatt tattgttccc agcttctttt ttgtatagac
    54121 ctatttctat ttttttatta tattggatat atatattcat tgaaaataga gcataacaac
    54181 tttataatgt tgagtatacc aatttttgga ggcgttttaa tggaccgcta tttagattta
    54241 ctttcagaga agttccctac aacggaagat gtcatcacgg aaatcatcaa tctcgaagca
    54301 attttgcagc ttccaaaagg aacagagtta tttttgagcg atatacatgg cgaatttcct
    54361 gcttttgatc atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt
    54421 ttctctgaac aaatgacgga aacggagatc agccaattca ctatttttac ggcttatcca
    54481 gaatatgtgt tgacgactga ttggtacaaa aaacaagata aatcccagct gatccatcgt
    54541 ttgatcgatc ttttacggtt tacgacagtc aaatacactc gatcaaaagt tcgaaaagga
    54601 ttgccgaaag aatatagcta tatcattgag gaactcttgt atatcgatga tcgtatcaac
    54661 gggaaaaaag actatgtaaa aaaaatcatt gaacaacttg tcttgatgaa ggaagaagaa
    54721 aaatttttaa caaaacttgc gcagacgatc caaaaatctg tcatcgatca tttgcatatc
    54781 gtgggtgata tttttgatcg aggaactcaa gcagccaaag taatggatcg aatcatggat
    54841 ttttcctctg tagatatcca gtgggggaat catgatattt tgtggattgg tgcgtattgc
    54901 ggctctgagg catgtttatt gacgctttta cggattgccg cacgctacaa ctatttattt
    54961 gaattagaga aagaatatgg attaaattta cgtccattat tttcttttgc ccatcaaacc
    55021 tatcagaaga atccagtatt tacccctaaa aacaggaaag atctatctga aagagagcaa
    55081 gaagaattag aaaagatcca tcaagcactt tctatcctcc aattcaaatc agaacatcag
    55141 ttgttgaatc gacgacctga atttaaaatg gacgatcgta aattgttgga aaaaatcaac
    55201 tatgaggaat caacgatcgt tttagaaggt cagctctatg agttgaaagg gacttgtttt
    55261 caaacgatcc agccagatga tcctaaaaaa ttcttggaag aggaacaaaa agtgatcgat
    55321 agtttgatgt attcattcca gcactcgtta agaatgcaaa aacacatgac gtttttgatc
    55381 gaaaaagggg gaatgtatct cacgaacaat cagcatgtac tgttccatgg ctgtatacct
    55441 gtagatgaaa aggggcagct cttagcattt gaattagacc aatcttatcg cggaaaagaa
    55501 ctgctgggat tttttgaaaa acagataact gaaagcgcaa aagatttgac tgcaaatgaa
    55561 gatttggcga ccgatttgat ttggtatgcg tggagcgggc cattttcgcc tttattcggc
    55621 aagagtaaaa tggcaacttt tgagcgctat tttctcacag agcctcatac gcacgtcgaa
    55681 aaaagcaatc cctattatca cttgcgagat gaagagtggt tcagtgaaaa aattttggca
    55741 gaattcgatg caaaagaaga aggttcagca atcatcaatg gacacacgcc agtaaaagta
    55801 aaaaaaggag agtcaccgat caaagcaaac ggaaaagcat ttgttattga cggaggtcta
    55861 tccaaagctt atcaaaaaac aaccgggatt gctggctact cattactatg caattcttac
    55921 ggtttccaaa tcgtgacaca ttatccgttc ttgcctattg aacaacaatt tgcaaaaaaa
    55981 tctgatcaaa cgaatgtaaa aaaagtgatc gagcagcagc tcccacgcaa acttaatcgg
    56041 aatacgacaa aaggaatgga attacagcaa cagattactc aactccaaag gctgttggat
    56101 tatcgaaagg acgattgaga aaaatgctag ccaacattgc tcaatagact aaaaagcgac
    56161 tgcaacaaaa ggcgtgtcgc agtctccttc ggaattagct caaaaaacgg taaaatatta
    56221 gaagctattt ttctgtttat ttctctaata ctcaggtcag ggcgctgatt tcacgacctc
    56281 ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaacaaaca
    56341 tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac
    56401 ctaatcagaa aaacgattaa aattcattca caaagaaatc gaataaacgc tgttcagatg
    56461 aatctacacg atgagaaagt tccggatgcc attggacacc aaggattttg tgcatattat
    56521 ctttactttg gactgcttca atgagtccat cgttggaaaa ggcgatggct tttaacaaag
    56581 gagaaagatc tttgactgcc tgatgatgat aggaattcac aaagtatgaa tcagggagaa
    56641 ggtcacttaa taaactgtcc gaaacgattt tgacttcgtg agttgcaaac tgcggggcag
    56701 tgggctgttg ttcgtgtttt acggaccatt ttggatataa tgaaagatct tgataaagtg
    56761 ttccctccaa cacgacattc aatagttgaa gtcctcggca gactgcaaaa atcggttttc
    56821 tttgtttcaa tgcttccaag atcaatgcct gttcgaattg gtctcgttga atatttgttt
    56881 ccgtcagttt tggatgaggg tcttccatgt aaagttgcgg actaacatcc tgaccgcctg
    56941 caagaatgag tttgtcgatt ttctcaatta gttgaggcgc tgaatctgga gacatgactg
    57001 gcagaataag aggtaatcct tgagcatcta aaactgcatc cacaaatccc tgcggtgtat
    57061 agcttacttg atttccttgg aaagtatctg tcgctcgaat cagttgattc ccggcaatgc
    57121 cgatgattgg tttcattttt attcccccta aagtagacta attgtaacaa ggatttaaaa
    57181 aaatttaaac tatgtaagac ttgcccctat acttatctgt tcttgctgtg ttactatctg
    57241 taaagagaat tttcgtgtga aattcatgac gatagttgaa aatattgctt aatttgttat
    57301 aataaaaagt aagcagaaat aaaaaggttg ggcgtaagct tcaaggatct agttcttcaa
    57361 ggggtgagaa ggacatttta ttgaaggggc ctgcctatac taagttatct ttagaggtac
    57421 atactttcaa tgaaaaaaga aagcatgtac ctctttttta aatcgaaagg aagtgaagca
    57481 agtgttagaa gctttggaac aaatccgaaa agcagaagaa gaaaatgaga agcgtgctcg
    57541 aaccctacgg cagcagttga agacctatgc agaagaaaaa gagcaagaat tggagataat
    57601 ccaaacgaac agtcggcaac agctgaaaca gttggtagaa gaaaaagaac aattcgaaaa
    57661 gcaagaatta gaaaaagaaa aaatgaaatt gaaagcagaa gcagacaagg tgcaagcaac
    57721 tttgaaagac caatatcaaa agcatcagag tcaagcaatc ggtgcaatca ttgaaagggt
    57781 gaaagaaact tatggccgtc actaagatgg aaaaagtgac cctcatttca gataaaagaa
    57841 atcaggagag gattttacaa gcagtacaag gaatccaaaa tgttgaaatc cgggatctgt
    57901 atcaaaatag aacaaacaac cagtgggtag aaaaatattt tccagatacc acgattattg
    57961 ataaagaagc aaaattaagc gctttaggca atcggctgaa agacatccaa gaagctatcc
    58021 aattcataca acaccacggc gatagccgtc aaaagaaact ccatttgaaa cgtcaagaac
    58081 tttccttaca agacttggaa gcaaattatt ccgaagaaaa tttcatgaat aaattagaag
    58141 aagtccttga tttaaaagaa aagtgggaac agctaactga aaaacgtgat cgtttagaag
    58201 aagaggaaga atggcttctt cattggcaac agctggatat tgctccaagt atgtatgcaa
    58261 gtaagacgac catttttgag atggggactg tgagtgcagc aaattttgaa agtttcaagg
    58321 aagaactaag taagcttgat gacgtttata tggaagaaat agattatacg ccaaaacaag
    58381 tttatctttc gtatgttgct ttaaaaaaat cagttgctgc tatagaagaa attgctggga
    58441 aatacgggtt caataaagaa acgtatcctt atgaacaaaa tcctaaagaa aaattgcaag
    58501 aaaccaaaca gcaattatcc attgttcacg agcagcaaaa aaagataacg acagcgttag
    58561 gtcagtgcag cggctatatc caagaatttg aatgggtaga agaagtcaca cttgccgttg
    58621 cagaacggga aaaagtaaaa gaacggttta tccatgccac ttatctgatc gttttgcaag
    58681 gttgggtaga tgcagaagaa aaacagaatt tgatgcatgt gttaaacgaa actgtatcag
    58741 aagaagatat atacgttagt ttcgaaacac caaactcaga agagattcag caggaagtcc
    58801 ctactaaatt gaaaaaccat ccgcttatcg aaccttttga attgctgact gaaatgtaca
    58861 gtctgccaaa atatgaagaa gtagatccaa cgccttggat gacaccattt tatcttgtct
    58921 tcttcgggat gatggtcgca gatgttggtt acggattgct gatgctcatt gggacaatac
    58981 tagctcaaaa actatttgtt ttaccccgcg ggatgaaacg tttcgtaaaa ttctttgaga
    59041 ttctctcggt accatcgatc atctggggat tgatctacag ttcatttttc ggacaggcat
    59101 taccaaaaga actatttggt atccgcttgc ctttcccaat cctatctacg acggatgacg
    59161 tcaatacgat tttgatttta tccgtgattt tcggtctgat acagatcttg gttggattat
    59221 tcgtggcagc aaaggaaaat atcaggagga aagattactt gagtgctgtc agtgatggtt
    59281 ttgcctggca agggatcctg attgggatcg ttattgcatt gattggtgtc atgttgataa
    59341 aaaatacagc atttgtttat cttggcgcgg ggctatcgat cattgctgct ttatgtatct
    59401 taatcgtgcc gattatccaa tcgccgtcta aagtcaaggg tgctgccaaa ggagcgtata
    59461 atctttacgg attgactggt tatatcggtg atttagtcag ttatacacgt ttgatggcac
    59521 taggtatttc aggcggaagt attggcgcgg cattcaatat gctagttgct ttcatgccgc
    59581 cagccgctcg attcagtgta gggatccttt tgatcatcgc tttacacgca ctgaatatgt
    59641 ttttgacact gttaagtgcc tatgtccatg gtgcacgatt gcagtatgtc gaattttttg
    59701 gaaaatttta tacaggaggc ggacgtgcct ttgacccgct gaaaacggcg gaaaaatacg
    59761 tcaacatcaa tcataagaaa aaaaacaata aataattgga ggatttttat acaatgatgg
    59821 attacttaat tagtcaaaat ggtggaatgg tatttgcagt attagcgatg gcaacagcga
    59881 cgattttttc agggattggt tcagctaaag gtgttggaat gacaggtgag gcagcagcag
    59941 cgttaacaac aagtcaaccc gaaaaattcg gacaggcttt gattctgcaa ttgcttccag
    60001 gtacgcaagg gttgtatgga ttcgttatcg cctttttgat ttttatcaac ttaggaaatg
    60061 atatgtcagt tgtacaagga ttgaactttc taggcgcttc tctgccgatt gcttttacag
    60121 gtttgttttc aggtatcgcc caagggaaag ttgcagcagc aggtatccaa attttagcga
    60181 aaaaacctga acatgctaca aaaggaatca tttttgctgc gatggttgaa acatatgcga
    60241 ttttaggttt cgttatttcc ttcctattag tattgaatgc ctaagcataa aggaggaaaa
    60301 gcagatgaac gccattgaaa aaatcatctc tcagatgaat gaagcagcag agcaagaaag
    60361 agcggcgtta gagcaagaag agcgaatgaa gatcgatcaa aactttgaac aaaaacgtac
    60421 gcaaatcgaa acagaacatc aaaaacaaaa agaaaaacaa atcgaattac ttgaaaaaaa
    60481 ataccgtcag cttaggaatc gccaacaagt agaagtacga caagaaaatt tgaatgcgaa
    60541 gcaggaattt ttacgacgat tgtttgcaga tgcaatagct gaaatggaga attgggatga
    60601 atctgaacaa atccagttta tcaaaaattc attgtattca ttacctttga caggcaaagt
    60661 aacattcatt gctggagaaa aatcagcagc ttatctttct cagacattgc tggatgaatg
    60721 gaacaatgaa cttcccttta tgatggtttt aagtgatgaa actgttgctg atcaagcggg
    60781 ttttctgata aacgatcaag gagttcagta taattttctt ttcagcagcc ttgtacaaga
    60841 tatccaagga acgatgagtt ttgagattgc caatcaatta ttcgaataag gagggatcag
    60901 tgtatggaat accacgaact aaatcccttg attcgcggaa gagagcttga acttttgtcc
    60961 aatgagacat ttgaacggat gattcaaaca aattctgtcg aagcacttgg tgaaatattg
    61021 aaatccacga tttacagtcc atatatttat gacggatatg aatatgactt cgaacagaat
    61081 cttttaaagg agcagagccg tctatttcgc tggttaaagg aatcagcacc ggagcctgaa
    61141 atcgtttgga tctatacgat gcgctatact ttccataatc tgaaggtttt gacaaaagcc
    61201 gagatgacaa atcaaaatct ggaccatttg tatatcgacg atggatttta ttcattagaa
    61261 accttgaagg atgccattca tacgcaagtt tcaaatgaac tccctgaaag cattatggac
    61321 agtatacgag aagtacgtga ttattttgaa gagtctacca ttcttcaggg aattgacgtg
    61381 atttatgatc gacagttttt gacagagcag cgacggcttg ccgataagct aggatatcaa
    61441 gaattgctga cagaagtcat tgcttttatt gacttgacca atatcacaac gatggctcga
    61501 ggaatattgc agaaaagaac atatgggttc atgacgacgg tactttccag ctcgggaagt
    61561 atcgaaaaag aagtattttt gaactttgta gataaagata tggaagatta cacgaatttt
    61621 ctattagaga cagattatgc tgattttctg cgccctgtca tccgtgaggg aaatcttgat
    61681 ctcgtaaagc ttgaacagtt gaaagatgat tatctaagtt cattgtatca agaagcacaa
    61741 acccaggctt tcggaccact gccactacta gcctttttga acgcaaaaga ggtagaaagc
    61801 aaaaaccttc gtttactggt tatcggcaaa agaagccatt tcgaaccaga aacaatcaga
    61861 aagcgggtga gacaagtcta tgacgcataa gatcggtgta gttggcgaca aagattctgt
    61921 tttgccattc aaattgtttg gctttgatgt tcgttatggt acaaccaagc aaaccgttcg
    61981 tcaggcaatt gaagaaatgg caaagaatga gtatggcgtg atttatgtca cagagcaatg
    62041 tgcagcattg agcccagaaa cgatcgaacg ttacaaggaa caaatgatac ctgctatcgt
    62101 attgattccc aatcatcaag gatcattagg gatagggctg gaagaaattc aaaaaaacgt
    62161 agaaaaagca gtaggtcaaa atatcttata gtatgaaaag gagatagaaa ttttgcaaat
    62221 tggaaaaatc ataaaagtct ccggacctct tgtaatggct gagaacatgt ctgatgccag
    62281 tatccaagac atgtgtctag tgggagatct aggagttatc ggagaaatta tcgagatgcg
    62341 tggcgatgtg gcgtctattc aagtatatga agagacttct ggaattggtc caggcgaacc
    62401 ggttcgctct actggtgaag ctttatctgt tgaattagga ccagggatca tttcacaaat
    62461 gtttgacgga atccaacggc cattagatac ctttatggaa atcacacaaa gtaatttctt
    62521 agggcgcgga gttcaattgc ccgcattgga ccatgagaaa aaatggtggt tcgaaccaac
    62581 agtagaagcc ggagaaacgg tgagtgctgg agatgttatc gggatcgtag aagaaacaaa
    62641 agttataaaa cataaaatca tggtgccaaa tgggataaaa ggaacgatca aaacaatcga
    62701 aaaaggttca ttcactattg atgaaacagt atgtgtcatt gaaaccgaaa atggtgacaa
    62761 aaatttgacg atgcttcaaa aatggccagt aagacgtagt cggccaatca aagaaaaatt
    62821 aaacccggat gcacccatga tcactggaca gcgagtgatt gatacattct tcccagtgac
    62881 aaaaggagga gcagcagctg tacctggtcc ttttggtgca gggaaaacag tcgttcagca
    62941 tcaaattgca aaatgggccg atgtggacat ggttgtctat gtgggatgtg gcgagcgtgg
    63001 aaatgagatg acggatgttt taaatgaatt ccctgaattg attgacccta atacaggaga
    63061 atcgttgatg gaacgtacgg tattgattgc taatacatca aacatgccag tagcagctcg
    63121 agaagcttct atctatacgg gaatcacaat tgcggaatat ttccgtgaca tgggttatga
    63181 cgttgctatc atggcagact ccacttcacg atgggcagaa gcacttcgtg agatgagcgg
    63241 acgtttggaa gaaatgcctg gtgatgaagg ttatccagct tatcttggtt cacggcttgc
    63301 ggaatactat gaacgatctg gaagagtcat tgcattagga tcagaaggtc gcgaaggaag
    63361 tataaccgca atcagtgctg tatccccgtc tggaggggat atttcggaac cagtcacaca
    63421 aaatacattg cgggtcgtaa aagtattctg gggtcttgat tccagtttag cccaaaaaag
    63481 acattttcct tcgatcgatt ggatacaaag ctattctctt tattcaacag aagtagggaa
    63541 gtatatggat catatccttc aagctgactg ggcatccatg gtcaccgagg gcatgcgtat
    63601 tttgcaggaa gaacagcagc tgaatgaaat cgtgcgtttg gtagggattg attccctttc
    63661 agataatgac cgtctgacat tagaagtggc aaaatccatt cgtgaagatt atttgcagca
    63721 aaatgctttt gatgatgtcg atacattcac ttcaagagag aaacaattca aaatgttgaa
    63781 gatgatcttg acctttggtg aagaaggacg ccgtgcgttg tcgttaggtg cttatttaag
    63841 tgagatcatg gacggtaccg tatcgattcg tgaaaggatc ggacgaagca aatatatccc
    63901 ggaaacagaa ttagaaaaac tagatcaaat tattggtgaa atcaagcaaa cgatgcaaac
    63961 gatcgtttca gaaggaggca tgagcaatga ttaaagaata tcgtacgatc ggtgaagtcg
    64021 tcggtccatt gatggcggtt gaaaaagttg ctggtgtcaa atatgaagaa ttaatcgaag
    64081 tccgtatgca aaatggcgag attcgtcgtg gacaagtatt ggaagtccaa gaagataaag
    64141 caatggtcca aatattcgaa ggaacaagtg gtatcaattt acgtgactct tctgtacgat
    64201 ttttaggcca tcctttagaa ctaggtgtat cggaagacat gatcggtcga gtattcgatg
    64261 gtttgggacg accgaaagat aacggaccag agatcctgcc tgaaaagcat ttggatatta
    64321 acggagaagt aatcaatcca gtagctcggg attatccaga tgagtttatc caaacaggta
    64381 tctctgcaat cgatcatttg aatacattag ttcgtgggca aaaattacca gtattttctg
    64441 gctctggtct tcctcataaa gaattagctg ctcagatcgc gcgacaagca acggtactcg
    64501 attcatcaga tgatttcgcc gtagtctttg ctgctattgg aatcactttt gaagaagctg
    64561 aatttttcat ggaagatttt cgtcaaacgg gcgcaatcga ccgttctgtc atgtttatga
    64621 atttagcaaa cgaccctgca atcgaacgta tcgctactcc tcgtatggct ttgactgctg
    64681 cagaatatct tgcttatgaa aagggaatgc atgttttggt tatcatgaca gacatgacca
    64741 actatgcaga ggcattgcga gagatttctg cagcacggcg agaagttcca ggaagaagag
    64801 gatatcctgg gtatctttac acgaacttag ctactttatt cgaacgagca ggaagaatcc
    64861 gcggactaaa aggatcggtt actcaaattc cgattctaac aatgccagaa gacgataaaa
    64921 ctcatccaat ccctgacttg actggttata ttacagaagg acagatcatc ttgactagag
    64981 agctttacaa gagtgggatt cagccaccaa ttgatgtact cccgtctctt tcgcggttaa
    65041 aagataaggg aactggaccc ggaaagacaa gagaagacca tgcagctacg atgaatcagc
    65101 tttttgcagc ctatgcacaa ggaaaacagg caaaagaatt agccgtagtt ttaggtgaat
    65161 ctgctttatc tgatgtagac aagatctatg cgaagtttgc tgagcggttt gaaaaagaat
    65221 atgtcaatca aggattctac acgaaccgtt cgattactga aacacttgat ctagggtggg
    65281 aactgctatc gatgttgcca agaaccgaat taaaacgaat caaagacgac atgctggaca
    65341 aatatctgcc agaagggaag tgagttctga tggcacgatt gaatgtgaat ccgacccgca
    65401 tggaattgac tcgattgaaa aaacagttga caactgccac aagagggcat aaattattaa
    65461 aagataagca agatgaactg atgcgtcaat tcatcctgtt gattcgaaag aacaaccagc
    65521 tgcgtcaaga aatggaagag tctatgacag aagcaatgtc tgattttgta ttggcaaatg
    65581 cttcgatcac ggaacctttc atcgaagaat tatttgtttt gccggcggaa aacgttgatt
    65641 tgtctatcgt agacaaaaat atcatgagtg taaaagtacc tgtgatgaat tttgaatatg
    65701 atgaacagct gacagacaca cctttggaat acgggtattt gaattccagt gccgaactgg
    65761 atcggtcaat cgatcggttt actaccctgc ttccaaaact tttggaactg acagaaatcg
    65821 aaaaaacttg tcaactgatg gcgagtgaaa tcgaaaagac acgtcggcga gtcaatgcat
    65881 tagaatatat gacgatccct caattagaag aaacgatcta ttatattcgt atgaagctgg
    65941 aagaaaacga gcgcgcagaa gtaacaaggt tgatcaaagt aaaaaatatg ggtaccactg
    66001 actaagtggt atcttttttt aaaagaggaa aacgatgatt aaacaaagca aacaaataaa
    66061 gaaacgtttg ccggctgttc agcttctcgc attaggattt tttctcctga ttttagttgg
    66121 cggcggccta cttaccctac cattttttag taatagtggg catgggacaa attttatcga
    66181 tgcgcttttc actgcaactt ctgctgtctg tgtaacggga ttgacaacat tgaatacagc
    66241 agagcattgg aacagtgctg gtcaattttt gatcatgctt ttgattgaga tcggcggtct
    66301 tggttttatg atggttccga ttctatgctt tgcattgata aagaagaaag tcagtctcag
    66361 cacacgtatt atattaaaag aagcgctgaa tctagaagaa gtatcaggtg tgatgaagct
    66421 catgctttac atcctaaaat tggcctttgt tatccaattg attggtacgg ttgctttatc
    66481 ttttgtattt gtgcctgaat ttggctggtt gaaaggtatc tggtacagtt tgttccatgc
    66541 aatctctagt ttttgtaatg cagggtttga tttgttagga gacagtttag ccaatcacca
    66601 gcaaaatgtt tatttgatca tggtggtatc tgctttgatc atttctggtg ggttaggctt
    66661 catcgtatgg cgtgatttac tggaatatca tcagaaaaag aagatgacga ttcattcaaa
    66721 aatcgcatta accgttacag gtaccctttt attaggcgga ttcgttgtgt tctttttcac
    66781 agagcgcaat ggggcaacgc tagtggaagg gaatatgttt caacgattgg caaacacctt
    66841 ttttatgagt gtgacaccta gaacggctgg atattattca gttgattacc tccatatgag
    66901 ccatgcaggt ctgattttaa cgatgttttt gatgtacatc ggcggaacat ctgggtcgac
    66961 ggccggcgga ttgaaaacaa ctacgttagg tgtgttgctg atccagatgc gttcgatgtt
    67021 gaaaggacgt acgagagcgg aagcatttgg ccgtaccatc cgacaaggcg cagtgttgcg
    67081 ggcacttact ttattttttg ttacgttgac gttgtgtgta gtcgccatca tgattttatc
    67141 ggtgactgaa acgataccta aaacttctgg catcgaatat attgcttttg aggtcttctc
    67201 tgcgtttggt acagtaggtc ttacgatggg attgacgcca gacttgactg caatagggaa
    67261 attgatcatt atagcgctta tgtacattgg acgagttggt gtattgactg tggtcttctc
    67321 tctgatgaca aaagcacaaa gaacacaatc aaaatacaaa tatccagaag aaagtgtcat
    67381 gattggataa tttcatcttc tgacagaaac ctagtgattt agtaaaataa gaaaataaaa
    67441 taatttttct tgcttcttgg atactttaag tgcaaaatgt tcgtttattt tttgaggcac
    67501 tacccgttgt tgaacctcgg gtagtgcctc ttttttattg aaaggaatca ataagatttg
    67561 attcctttat ttatcaatag gttataattc atattttttt attttttctt ttacgatact
    67621 tagtccagaa gctaaaattt caaggttatt agaggatagg gaaattcgaa cgaatgcatc
    67681 tggttgcgca cgtgtagtaa agcgtctgga atgataaagt cggataccgt tagcaagaaa
    67741 gtcattctca attatcttag ggtcaatctc aggatcaact ggaagaattc gatagtaagg
    67801 gacacgattc gttggttttt ctactttgaa aagagaatca aaaagctgat tggctctttt
    67861 tgtaagactt aatttagttt ctaagatcct atttgcttca tttgaatgga ggatttgagt
    67921 gatgacttcc acatctaatc cagaagtttt cacatttata ttaaaaattg cttgttctat
    67981 cttgctttgg acatgtactg gaactacaag ataagcaact cgcaatccag aacaaataaa
    68041 ttttgtcatt ccagctaagt agattgtttg gttaggtaat aaattttgga aaggaggaat
    68101 gatcgtatgt tgatagtaag ttgttaaaaa tgaatggata tcatcttcaa taacccatag
    68161 accttctttt tcaatcactt cggctaattg ctctcgtctt ttgtgagaga tttgaaaccc
    68221 tataggatta ttacaagaag gcataagaaa gacaccatga atcttctttt ttctacattc
    68281 ctgaatcaat atatcaggtc gcattccttg ctcgtcaaaa tcaattggaa cgatctctaa
    68341 atgatataac tgagccaatt caatgaaatt agcgtatgta tatcggtctg ttgctattct
    68401 atcacctgga gagaaaagtg cagaaagtgt gatagccaat ccattttgaa caccggaaac
    68461 aatagataga gattctaagg acggaatgac tccttgagtt tttaaccaat ttgctgcagt
    68521 agtcaattga aaatttgtgc ctttgggatc ttggtaatta agcaaaccaa caagatttgg
    68581 gttattacta gttgattgaa taaacgggac aacgaattta ttacattctt caaaagaact
    68641 aaccagacca aaatcaatga tatgttcggt tacttcattg gtagagatgg tgatggactc
    68701 aaagacattg ggagaaataa atgtgccact tccaatattc gtatacagca aacctttctc
    68761 aattccatat ttataagcct tgccaacggt agtaaaattt aaatctaaat aatcagctag
    68821 ctctctttgg gagggtaact gagtgttttt ctgtaattta ttctttaaaa tatcttgttc
    68881 taattgttga atcaacgatc gataataagg acgcttcaat ttttgtttgt ctggtcgcca
    68941 agttaacggg taattgtcat aagaattaat aggcattttc tccactcctt aaaattgtat
    69001 accatacaat tttaccattg attgtattga ttttagaagt taatcttttg tgaggaggga
    69061 attgtctatg aaaattatta attttgagga agtagtagct tcttttagtt ttgaagaagc
    69121 gattcaagta atgaaaaaat gtttttttga ttatgaaagt ggtaaaatca gccaaaatcc
    69181 tcgattggtg gaaattttac ctgacggaaa aggcaaaaat atgtttgcga tgatgcctgc
    69241 ttatttgggg gagaacagat attttggcac aaaagtcatt accgcttttc cggataatca
    69301 tcagtatcat ttaccttctc atttaggaga gattttcttg tttgatgctc aaaacggact
    69361 acccaaagca ttgataaacg caaatgcagt cacctggatt cgaacagctg ctgtatctgc
    69421 gctggcaaca gattacttag caaatccgca tgcagaaaca cttgctttaa ttggagctgg
    69481 tcaacaagca ttgtcgcatt tgactgcgat ttctaccatt cgtcccatca ctacagtgtt
    69541 tgtttacgat attttagaag aaagacgaaa tacttttatc gctaaagcta aaaaacagta
    69601 tcctaaaata acgtttataa atgcaatatc agtagaagat gcagtacgtc cagcggaaat
    69661 catttgtacg ttgacctcta gtaaagaggc atttcttcaa aaagagtggg taagggaaga
    69721 cgcacatatc aatgctgtgg gcacatttac tcctgataca agagagataa gtacatcgtt
    69781 gatggaaact tctcaaatct atgtagatga ttactcagct gccttaacag aaagcgggga
    69841 cctgcttata gccatggcag aaggagattt agaggaatct tgtattgttg gtacattagg
    69901 cgaattagtt tctgagaaaa aaacatttta taaaggagaa tcgaggccga cgattttcga
    69961 cgcagttggt ctagctattg aagatctttg ttgtgcagaa tatatttata acaaattaga
    70021 gaaaggggct ttaaaatgaa cttagattat tacgtagttg atgcatttgc agatgaggtg
    70081 ttcaaaggaa atccagcagc ggtttatgtg ctagaagaat ggctgccaga aggtacgatg
    70141 caaaaaattg ccattgaaaa caatttatct gaaacagcat ttacagtgaa gaaaaaccaa
    70201 gaatttgagc ttagatggtt tacgccagat cgagaaatcg atctttgtgg tcacgcgaca
    70261 ttagcaacag catttgtttt atttaactat tacaaaatac ctgatgaaac gattaaattt
    70321 tctactcaaa gcggtaatct atttgtgaca agaattaaag attattatta catggatttt
    70381 cctagtatta tgccgaaaaa agtgccgata ttgtctgaat atgaagaagc gattggggct
    70441 aagattaaag aagcctactt agcacgtgat ttatttttcg tattagaaga tgaaaaaacg
    70501 gtggctaaat tgcagccaga ttttacagct atcaaaaatt ttgaattagg aataggagtc
    70561 atcgtgacag cagaaagtgt gcaaaaagac tttgtttcta gaaccttctt tccaaaatta
    70621 acaatcaatg aagatccagt atgtggatca gcacattcta atcttattcc ttattgggca
    70681 gaaagaatga ataaaacaaa attgtcagct tatcaaatgt catcaagggg cggggatcta
    70741 gaatgtgaat taaaagaaga tcgtgtgata ataggaggaa aagcagtttt gttttctaaa
    70801 ggtacctcgt ttatttgatt tctttcaaat gaattaaaaa taaattggtg gagagtggca
    70861 accttatagc tacaatggtt gttaaaaaaa gacgcaaact ttttcaattt aatgaaaagt
    70921 ttgcgtctca ttttaattat tggtgtttgt tttttattca atcaagagta aaatgcccca
    70981 tactaactat cctcggatga cttcgatttt ataaccatca gggtcaatga caaagtagta
    71041 agaaggcgca gtgccaggta atccttttaa gtcagtaatc tcaagaccgg cagctttatg
    71101 ttgctcgtgt aatttttcta gatcatcagc tgctatagcg atatgaccat agccgtctcc
    71161 tagatcatac gcaccatgat catagttgta agtcaactct aattcatagt catccccagg
    71221 aagtgtcaaa taaacaagtg taaatttgtt ttctggaaaa tcacggcggc ggctttcttc
    71281 aaaaccaaat gccttttgat aaaattcgat tgatgcttca agatctttca ctcgaacaca
    71341 tgtgtgagcc attttcatag attcatctct ccttttcctt ttactcaatc ataacaaaaa
    71401 gaagtaagga aatagttgat ttgtgcttgt tcatgagaaa tttctcatgt aaaatggata
    71461 gaaaataaaa ctgttaaggt ggaaactatt catggataaa ccaatttttg gtgaaaaaaa
    71521 gcaatttgct gattatcaaa cccgttatgc agcatatatt gtcatagaaa gagatcagca
    71581 gattgcagta atcgaggctc caaatggtgc tttttttctt cctggcgggg aaattgaagg
    71641 agaagagacg aaagaagaag cgattgtccg tgagctgata gaagaaatgg gaattgtggc
    71701 agaaatttct tcttatctgg gagaagctga tgagtatttt tactcgaatt atcgtgcaac
    71761 ttattattat aatccaggat atttctttgt tgctgattct tggaagcgta taggagaacc
    71821 aactgaaaag accaataaaa tttggtgggt gagccctcaa gaagcgcttg tgaaattgaa
    71881 acgtggtagc caccgatggg cagtcgagag atggatcgaa aaaaataaaa actgaaagaa
    71941 agcgagtaga tcagctttct ctcagttttt ttatttatta cagtacatat tttttgattg
    72001 cttcagctac accgtgctcg tcgtttgatg aagtgatgac atttgctgct tctttgacaa
    72061 gagaaacggc atttcccatt gctactccta gaccagcata ttcaatcatg gataagtcgt
    72121 tctcgttatc tccgatagcc atgatttcat cttgatcaat accaaggtgc tgtgcgagat
    72181 tcgcaactgc cgcaccttta cttgcttctt tatttagtac ttcgaagtaa tagggcgcac
    72241 ttttgaccgt tgtgtattta tcacggaaag tttgcggtaa acgcgcaatt gctgcatcta
    72301 aaatttctgg ttcgtcgatc atcatcattt tgacgatgtt catgtctggt gtcatttctt
    72361 caggtgttcg atattttagc ggcatcttga ctaaacttgc ttcgtggatc gtataagcgc
    72421 tgatatctcg attaggcgtg tagatcgctt tgtcatcgat cgaatggaga tgactgccga
    72481 cacggcgtgc catcacttcg atctctagga aatcttcatg tgtcagtccg tgacgggcga
    72541 tgattttacc tgatttcgtt tgttgtacga gtgccccgtt ataagtaata acgtaatcat
    72601 tatcttgaaa aaggttcaac tcgtttaact gttcctggac acctggtaag ggacggccag
    72661 tgcataaaac gatcgaaaca ccttttgcaa cagcagcggc aatcgcttct tttacttctt
    72721 ttgttacttc tcttttctca tttaataaag taccatcgat gtcgatagcg actagtttga
    72781 tagacataat ttcctccaat atattttaat ttatttcaat caatgcgcca ttacggatat
    72841 ggctggcaaa ttcttgataa gtttcgtcaa ataaatcata atgatcacgc aaggacggat
    72901 caaccatttc ttttggaaag aaaaatcgct catctccttg ttcttgtccc gctaatgcag
    72961 caacaagctg actaacctgt gataattcta tcaaagttcc gtcatttcgc aaaatctcga
    73021 tttgtgtgcg atgtctgcct tgttctgggc gataaaaatc ataaggtaga tcgtagctag
    73081 aattcacagc agtgtaataa acaggattat atccgacctt ttctactaac aaagtgagtt
    73141 cttggactaa ttttgaatcc cggttgccag aaaaagtagc agatttcagt ggtttacgat
    73201 tcaaaaaacg tttagctaaa tcatttaaaa taggatcagc agattcagac cattgagtaa
    73261 agtaagtgcc caagacccca tcatcaagtt tgaggtattc ttctaaagta aagcttccgt
    73321 caagaaatgg cagaagtaat tgagaatgga gttcaaaact gctggcatct tcttgataca
    73381 actcgtgtgc gcgatgaagc agatgctcca aaattacttc cataccacgg gaaacaggat
    73441 ggaagtacac ttgcacatac atttgatatc ggctaacgat ataatcttcc actgcatgca
    73501 tgccactcat ggaaaaagcg atcccgcctt cataaggacg aatcacacgt aatattcggg
    73561 taagatcaaa tgttccatat tctgttccag tgaaataagc atcccgaagc aaatagtcca
    73621 ttcgatctgc atcgatctga ctagaaatca tttggacaac ttgtgggttg gggtaagttt
    73681 tttggatcac actagctacc ttttcaggaa aaccttcttc tactcggtta agaatctgat
    73741 acacttctgt ttctggagaa gtgatgatct ctacagtgat ggcttcatga ttcgtatgga
    73801 agatatgttc aaatgtatga gagtaaggac catgaccgac atcatgcaac agtgccgcac
    73861 ataaagtcac cagccgttcc tgatcatccc agcctccgtt gcctattttt tcttttgaaa
    73921 aatttcgaga aaagatatcg cagattcgtc ttgaaatctc ataaacgcct aatgaatgag
    73981 taaaacggct atgttctgct ccgtgaaaag tgaaagaaga agtgcctaat tgtttaatgc
    74041 gtcgtagacg ttggacttct ttcgaattga tcaaatctaa gattacttga tgctggacat
    74101 ggatatagtt atggactgga tcacgaaaaa ctttttccat aggcagcatt tgatatttat
    74161 aaggggttgt cataatgtag ctcctttatg ttattacgtt gttccattct ctggatttgg
    74221 ttgttccatt gctccagctg gatttctttt tttagccagt cggtagaatc ctgtaaatca
    74281 cttgcagaat acagcttttc aaaagctttt gcaaatgctt ctttgacttc ttttacaaaa
    74341 aggggttggt caagcagttc ttcaagtgtt gccattgtag caggatccac tgcaggatag
    74401 ccatttgtac caaagtcttc ttttaaagca gattgataaa atcgcttcat gacttgcccg
    74461 cgtttttcct gattaccgtt gacacttaag tacatcataa cagaaatccc atttttgatc
    74521 cgacgctgag cgataccggc aaattttttg ccatggatgc ttaaatcata agttccagga
    74581 caataagaat gggtgatttc gaatgcttgt atcggtgcct ttggaaaagc gtatttagtc
    74641 aataggagca tttgctcgta agcgtcatct atggttattt tttttgtgtc actttggggg
    74701 aaaatcaaag aaatattcaa aattccttca tctgatacga tccctaatcc acctgaattt
    74761 ctgatcactg gagcgtaacc tgcatctttc agtgtttgga tcccgtcact tagaaaagga
    74821 gttcgaacat ccttcatacc taagatgact gtgcagtcat attgccagaa atgtaaaaag
    74881 atttgctggt gttctttaga atattccgtt aatatatctg ttaatgcaaa aggggaaaag
    74941 tcgttttttt gataaatgtg ctgatctagt cgaatcgcag ggttcattca accggctcct
    75001 ttgtataaat tcatattttc tacaatatta tactctaaaa tcaaagagtt gttttggaaa
    75061 aaaggttttc tgtaaaaatg gacaagttgg ttgacaaaac aaaagaaact aggaacaatg
    75121 tagctatgag aagtaaagga gaatgaataa tggaattatc acaaggaaca ccgatttcaa
    75181 tcaaattgcg tacaaaagta aaacagcaag gggaagtgca agatttttat tttgatttga
    75241 aagggcaaat ggtgacaatt ggcgatacgc tgtacatacg ttataaagag attcaggagg
    75301 agacgggcga tgagattcct gtaacgatca aattagttcc agatggtcat gtacagctga
    75361 tccgtgcagg agaaatgcgg atgcgtttga aatttggcta taaagaacgg ttagaaacat
    75421 cctatcgaac accatacggt atgatccaga ttgctacttt tacgaaagaa ctgcatgtta
    75481 gtttgaaaga ccgtcccaca ggagggaagg ttcgaatcga ttatgatctg ttcatgggac
    75541 cggaaaaaat cggcgaatat tatttgacct tggattttac agcgtaaata taaaagaaaa
    75601 gtttgatttt ttgtgtctga ttttgtatga taaggagtag actgtgaaag gacgtgtcct
    75661 gattggaaat tagtgtattt gaaggtgcaa acaaaagcga attatcaatg atcgaagtag
    75721 cacacgcgat cttagaacaa cgtggcgatg tcatggattt ttctgattta gctaaccaga
    75781 tccaaaacta tcttgggaaa tcagacagtg aaattcgtga ttcactcgca caattttata
    75841 ctgacttgaa tattgacggc agctttatct cattagggga taatcgttgg ggcttgcgtt
    75901 catggtatgc tatcgattcg atcgatgaag aagtaaacca tggaatggac gaagaggatg
    75961 aagatactcc acgtcgccgt aaacgcaaga aagtcaacgc ctttatcaat gatgatgaag
    76021 acgcgatcga ttacaatgac gatgacccag aagatacaga tctgacagaa gaagatgacg
    76081 atgatctgtt tgatgacgat gatgatgaag atgaagaaat cgctgcttat aattctgact
    76141 tgcaagaaat cggagcagat gacccgacag atgatgaaga agacttgcca ggaaatatcg
    76201 aagaagattt aagtatcata gacgaagacg atgatgatga agattatgaa gaggaagaat
    76261 tttctgactc tgaaaaagaa taaacattat tttcttacga gaggttgtga cagagacgat
    76321 cagcctatca gaataaggag gtgtgacacg atttttgtca caccttcttt tttgtatata
    76381 aaaatcccaa gtaaatacat aaaaaataaa aaaattgaaa cttttagcgc accttgtacg
    76441 tcatattaag tgaggcagaa aggagtggag aatgattttt actggatata agaaaaaacg
    76501 gcagatcgaa aaataccgtc acctgctgaa tggactcatc gaaactgatt atcaaaaaat
    76561 gttccgtatc acgatgaatt acgtacatga taaagaagag gccttagatg tgatgcaaga
    76621 tagttttcat aaagccctga actattttga aaaagaaaaa gagatcgagc attttagtgc
    76681 atggttttat cggatattga tccgcacggc attagatagt tggcgcagac aaaaaaggac
    76741 gcctgtggac ttatttgaaa ttcaagatat cccccaacag ttacctttag aaacttccgt
    76801 tgcagagctc catatgattt tagataaaat agacagtccg gaaaaagaga ttctgatcct
    76861 ctactttttt gaaggatttc gtttgaaaga aattgcgact atcttggata tgaatgaaaa
    76921 cacagttaaa acgaaaatgt accgctcatt gaatgcttta cgtcaaatcc tagaatgaag
    76981 gagagaagag tgccatgtat tcccaaaaag aaaatttaaa aaaactaaag aaaacttata
    77041 aagaacaagc aattcccaaa gggcttaaag aggagatccg ccagcgtttt attatcgaag
    77101 agcaatcatt tataaaagca aagcggagaa agagacgtct ccaagggttc ggagtatctt
    77161 tggctatcgt aatgattaca acaagtagcc ttctttttaa tagtcaagtt cgaagctttg
    77221 cagaagattt acccatctta ggatcagtca ttgaattgat cttaggcgaa cgctttactg
    77281 atcgatctga aaaaatcgat attcaggtac cgaagatcaa tacacaaaat gagaaagaaa
    77341 ataaaacaat tcatggatta aatcaaaaat actttcgcga aggacaagca gattttgaaa
    77401 aagctgaaaa agaatatgga aattttgaaa cggatcatta ccaagttttg ggagattatc
    77461 agaaaatcgt agacgataac cggtttctcg tgattgaaag gcagatcacc caaacagcag
    77521 cagatagcca tgttgaaaaa agatacgaca caatcgacaa gaaaaacagt gtccagctct
    77581 cacttccact tttattcaaa gatgacacct atctatctgt tttgacaaaa gaagtcaaac
    77641 ggcagatggc cgaacaagtc aaggaagatc catctaagta ctattggact gagcaagata
    77701 ttcaagaagg gacgatagaa aaaccaacac ttgttacgcc tacacgaagt ttttatttga
    77761 acaaagagca tcaattagtt ttgacttttt ctcaatacga aattgctcct ggttatatgg
    77821 gtacaccaga atttgtcatt cctaagtccg tgacaaaaac gattttggca tcggaagatt
    77881 atttggatca ttagacgaga agtagttgcg actagcagtt tcaattttca agtaagtaca
    77941 agtaaataat aagaagagaa aaagagaaag aagagatata gtgaaaagaa attatttagt
    78001 tatcagtatt attgctttat ccgtgctttt gtttttcgaa attacttcgg tggcctcctt
    78061 gatcaccgaa gtaaaaggac aaacacactc ttcggagcat tcgtcacaat ctttcaaaca
    78121 atcagaacaa tcaaccatta aaagccaatc aactgaaaca gctaacgtca aagaagccat
    78181 tcaagaatca gcggaaaagg tacaaagtca gtcatcacaa tcaacacaag acagtacaaa
    78241 aacgagccga gatccggctg tcaaaagcgt cgcaatcagc tttgatgatg gaccaggagc
    78301 gaccacaaca ccgcagcttc tgcgaatctt aaaagaaaaa aacgtgcatg ccactttttt
    78361 cgtcttagga gaaaatacgg cgcagcatcc tgaaatcgtg aagcagacag ctgaagcagg
    78421 gcacgagatt ggcaattaca catacgatca tcagaatctg gccactcttt ctgcccagtc
    78481 gatgacggag gaagtgacga aagcagacac tgagatcaaa aaagtcactg gcaaaacccc
    78541 cacttttgtt cgtccgccct atggttctgt cacgaatgta ggagcaacga tcatccagcg
    78601 accgatcatc gaatggtctg ttgattcaga agattggaag acaagaaatc ctgatttgat
    78661 tttgcagaag atccaagcaa cagtatacga tggggcaatt attttattcc atgatatcta
    78721 tcctgaaacg attcgtgccg ttcctcaagt gatcgattat ctgaaagagc aagggtatcg
    78781 catcacgaca gttggggatt tattagggca tccaacggct gtcgagaact attatggcag
    78841 aaacgatcat cggccagttc agtaagtgag acaagtatct ttaataaata acacatttta
    78901 ataagtagtt gaacacataa ctaccttact tgcaaaggct taacaatatc tatacaggta
    78961 caacaaaaaa ctgtccacca attttctttg gcggacagtt ttcacttaaa aataaagaag
    79021 caactattcg taaattatgc taaaatagca gtagaacgcg tcctgaggga atcgaacccc
    79081 cgtctcaaga accggaatct tacgtgatat ccactacact aaggacgcaa gcactgggat
    79141 aattgtaact aaacagataa aaaattacaa ggggattgga caaaaaaatg aaatttgagc
    79201 aaaatttttc acaaaaacag cagcagacac agaaactagc catgacgcaa cagttgcaac
    79261 agtccatcca agttttacaa tataacagtg aggagctgtt ggcattcgtt gagaatcaag
    79321 caatggaaaa tccattggtg gaagttgttg agccggaatg gcagccagat tacataaaag
    79381 cttcttcatc ttcttatgaa ggagaagaga caaattacct aaatcagatt cctgatacaa
    79441 aagggtcttt atttgattca ttgatcgaac aagtccattt gaattaccga gatacttttt
    79501 tgaggaaaat cgttctttat cttgtagaat acatcgattt gaacggattt ttgacgatta
    79561 gtttagaaga agccggaaaa gagattggag ctacgcctat ccaaatgctc gatgcgttga
    79621 ctctgattca gcaattagaa ccagctggag taggtgcacg tagtttgcaa gaatgtctca
    79681 tgcttcaaac agaacgagat gattatgcgc cggaattagc ttatatcgtg ttggaagaat
    79741 gttttgagga actggtggag cgaaaatgga aagaaatcgc acaacgtttt tctgttgatc
    79801 tacatgctgt tcagcagata ttcgactatt tacaaacgtt gagtccaagc cctggccgga
    79861 ttttcgaccg tagcagcgaa ttgtacatta ttcctgatgt ccgggtatta gtggacgacg
    79921 ataaaaatgt acaagttatc tcaaaccgaa aaaatcagcc aaatatacgc tttcaagaga
    79981 gttacttcaa acaaatgtca caacaagcag ataaagaaac tgaaaattat ttaaaagaac
    80041 ggaaacagga attcgaatgg ttgaagaaaa cgatcctaca acgtggggat acaattttgc
    80101 gggttgctca agtcattgtt tcccggcaaa aagctttctt tatcgacaag gaacgtccaa
    80161 tcaagcctct tacgttaaag gaagttgcaa ctgagataga tgtgcatgaa tccacggtca
    80221 gtcgtgcagt caacgggaaa tatttggaaa cagatttcgg tgtatttgaa ttaaaaaaat
    80281 ttttcactac acgaattcct acgaatagta cagaacagac agaagacctt tcagcagata
    80341 cagcgaagaa aaagctgcag gaattagtcg atcaagaaga caaaaacaag ccgttgtcgg
    80401 accagaaatt ggttgaattg ctgaaaaaag atgagatagc gatctcaaga cgaacagtag
    80461 caaaatatcg tgatttatta ggaatcccga gttcatcaaa aagaaaacgt tatgataact
    80521 aatcagtcaa aagactagaa aaagtatttc actttttcta gtctttttag ttgaaaaacg
    80581 agagcgaaac tgttagactt acagtcgtga ggttgagatt tccctttatt gatgtgaaat
    80641 tcgaatagtt aatttctttt tttatcgtat atgggtcgtt ttaagtctac attggacgta
    80701 aagtgtccaa ctaaggagat cgctatgctg gatgaaataa aaatgattga atctgttgca
    80761 ccagatatta tcgaagtgtt gcaagaacgc tataaaattt tacgaaatat ctattggatg
    80821 cagccgattg ggcgtcgaag tttatcggaa agtatgggga tcacagaacg agtcttacga
    80881 acagaaacag accttttgaa aaatctgaat ttgatcgaca cctcaaaaag cggtatgacg
    80941 ctgacaacta agggcgaaga agtttatcaa agccttgaaa acttcatgga tcagctttta
    81001 ggtacgcatc aaaccgaaca acagttggca gaatattttg gtaaccaacg ctgtatcgtt
    81061 gtttcaggaa acagcgaaga gcagaccaaa gtggctgatg cattcggcga agcgctgagc
    81121 gaagccttag accgactact tccagaaggc gagaatatca tcgctgtgat gggaggaact
    81181 actatggctg tggtagccga acaattgtct aatctggaaa ataagaaacg gcataacctg
    81241 tttgtccctg caagaggcgg gattggtgaa gcgataactg tacaggcaaa ttctgtcagt
    81301 gcaagaatgg ctgctaaagc aaacggtaac caccgcgcac tttatgttcc ggaacaatta
    81361 agtctggcaa cttacaattc tttactcaat gagccatcta tccaagaagt cttaaacttg
    81421 attagtgaag cgaattgtgt tattcatagt attgggcgtg cattacacat ggcagcacgt
    81481 cgtaagatga ctgaaaaaga attagtcatg ctgaaacaag caaatgctgt tgcggaatct
    81541 tttggctact ttttcaatga aaaaggtgaa gtcgtttata aggtccctag aatcggcata
    81601 gaattgcaag atttggaaaa agtcccgatc atcatggcaa tcgcgggcgg aaaatcaaaa
    81661 gctaaagcaa ttcgagctta tatgaaaaat gcaccaaaac aaacgtggct catcactgat
    81721 gaagctgctg caaatgagat tttaaaaggg gtaacccttt aaaataaata tttttttgat
    81781 tttcataagg aggaaatctt aaatgacagt taaagtaggt attaatggtt ttggacgtat
    81841 tggacgtcta gcattccgtc gtatccaaga tgtagatgga atcgaagtag tagcaatcaa
    81901 cgacttgaca gatgctaaaa tgttggcaca cttgttaaaa tacgacacaa ctcaaggacg
    81961 tttcaacgga acagttgaag ttcatgaagg ttcattcaac gtaaacggaa aagaagttaa
    82021 agtattagct aaccgcaacc cagaagaatt accatggggc gaactaggcg tagatatcgt
    82081 tctagaatgt actggtttct tcacttctaa atcagcagct gaaaaacatt taacagctgg
    82141 tgctaaacgc gttgttatct ctgctcctgg cggaaacgat gttccaacaa tcgtttataa
    82201 cacaaaccac gaaacattaa ctggtaaaga aacagttatc tcaggtgctt catgtactac
    82261 taactgttta gctcctatgg ctaaaacatt gaacgacaaa tttggtgttg ttgaaggatt
    82321 aatgacaact atccacgctt acacaggtga ccaaatgact ctagacggac ctcatcctaa
    82381 aggtgacttc cgccgtgcac gcgctgctgc tgcaaacatc gttcctaact caacaggtgc
    82441 tgctaaagct atcggtttag taattcccga attgaacggt aaattagacg gagctgctca
    82501 acgtgttcct gtaccaacag gttcattaac agaattagta acagttcttg aaaaagaagt
    82561 aactgttgac gaaatcaatg cagcaatgaa agaagcttca aacgaatctt acggatacaa
    82621 cacagacgaa atcgtttctt cagatatcgt tggtatgact tacggttcat tatttgatgc
    82681 tacacaaact aaagtaatga cagttggcga caaacaatta gttaaaactg ttgcttggta
    82741 cgacaacgaa atgtcataca ctgcacaatt agtacgtact ttagaatact tcgctaactt
    82801 ataagataga caaacgtaca agtgtaaatt atagacatgt acatttaaaa gcggggaagc
    82861 aatcgcgctt cttccgcttt ttttgataat aagtttattc aggaggaaat aaaatggcta
    82921 agaaaacagt gaaagatatc gatttaaaag acaaaaaagt tcttgtccgt gttgacttca
    82981 acgttccttt gaaagacggc gtgatcactg acgacactcg tatcaaagca gcattgccaa
    83041 caatcaacta cgttctagaa caaggcggaa aagcaatcct tttctctcac cttggacgtg
    83101 taaaaacaga agaagataaa gaaggaaaat ctttagctcc agttgcaaaa cgtctaggcg
    83161 aattattagg taaagaagta acattcgtac ctgaaactcg cggagaacaa ttagaagaag
    83221 caatccgcaa catgaatgac ggcgacgttg tcgtattcga aaatacacga tttgaagata
    83281 ttgacggtaa aaaagaaagc aaaaatgata cagaactagg taaatactgg gcttcattag
    83341 gcgatgtatt tgttaacgat gctttcggta cagctcaccg tgcacatgct tctaacgtag
    83401 gtatcgcttc aactggtatc ccaacagttg ctggattctt gatggaaaaa gaaatcaagt
    83461 tcatcggtga agcagtagaa gaaccaaaac gaccaatgat tgcaatcctt ggtggcgcaa
    83521 aagtttctga taaaatcggc gtaatcgaaa acttgattcc taaagcagat aaaatcttga
    83581 tcggtggcgg aatgacttac acattctacg aagcaaaagg aatcaagatc ggtaactctc
    83641 tagtagaagc tgataaagtg gaactagcaa aagaattaat cgaaaaagca ggcgacaaac
    83701 ttgtcttgcc aatcgacaat gtctgtgcac cagaattttc aaatgatgtt gaaacacaag
    83761 taatcgaagg agacattcct gatggtttga tggctttaga catcggccca gcatcagtaa
    83821 aattatttgc tgatacatta caaggtgcaa aaacagttgt ttggaacgga cctatgggtg
    83881 tatttgaaat gagtaacttt gctaaaggta caatcggtgt ttgtgaagca atcgctaact
    83941 tagaaggcgc aacaacaatc atcggtggcg gagactcagc cgcagcagca gaacaattag
    84001 gatttgctga caaattcaca cacatctcaa caggtggcgg cgcaagcttg gaattgcttg
    84061 aaggtaaaga attaccagga ctagcagcaa tcaacgataa ataaacgacc aagtttgctc
    84121 aattctgacg agtaatcgaa caaaaggtga agggagtttt catgactccc tagccatttt
    84181 tcaataaatt aaggagtgtg ttcctcaatg cgtaaaccta ttatcgcagg taactggaaa
    84241 atgaacaaaa ctgcatcaga agcaaaagaa tttgctgaag cagtaaaaaa caaaatccct
    84301 tcaaatgaca aagttgattc agtcatcgga tctcctgcat tattcttaca agaattaatg
    84361 acatcagctg aaggtacaga actaaaaatc tcagcacaaa actgctactg ggaaaactca
    84421 ggtgcgttca ctggtgaaac ttcaccagca gctcttgctg atctaggtgt tcattatgtg
    84481 atcatcggac attcagaacg tcgtgaatat ttccatgaaa cagacgaaga catcaataaa
    84541 aaagcgaaag ctatctttgc aaatggtatg acaccaatcc tatgctgcgg cgaatcttta
    84601 gaaacctatg aagcaggcaa aactgctgaa tggatcgaag gacaaattac agctggttta
    84661 gctgatcttt cagctgaaca ggtgtcaaac atggttattg cttatgaacc aatctgggca
    84721 atcggtactg gtaaatcagc tgacgcacaa atcgctgacg aaatttgtgg agttgtacgt
    84781 caaacagttg aaaaacttta tggaaaagaa gtatcagaag cagttcgtat ccaatacggc
    84841 ggttctgtaa aaccagaaaa tattgctgaa tacatggcaa aagaaaatgt tgacggtgct
    84901 ttagtcggtg gcgcaagctt gcaagcagat tcattcttgt cattgttaga cgctgtaaaa
    84961 taattgttga aaatttgcga aaaatgcaag aaatgtttgc actaatgagc aaaattaact
    85021 aaaatcgtat atgagggaaa tcccttagga aaaaacaaac tcaaaggaga gacaaaacat
    85081 gtcaattatt actgatgttt acgctcgcga gatcttagac tcacgtggta acccaacaat
    85141 cgaagtagaa gtatacacag aaagcggagc ttttggccgc ggaatggttc cttcaggtgc
    85201 ttcaactggt gaatacgaag cagttgaatt acgtgacgga gacaaagctc gttatggcgg
    85261 taaaggtgta actaaagctg tcgacaacgt aaacaacatc attgctgaag caatcattgg
    85321 ctacgatgtt cgtgaccaaa tggctatcga caaagctatg atcgctttag acggaactcc
    85381 taacaaaggt aaattaggtg cgaacgctat tttaggtgtt tctatcgctg ttgcacgtgc
    85441 agctgctgac tacctagaag tacctttata ccactaccta ggcggattca acacaaaagt
    85501 attgccaaca ccaatgatga acatcatcaa cggcggatca catgctgata actcaatcga
    85561 cttccaagaa ttcatgatca tgcctgttgg cgctccaaca ttcaaagaag ctctacgtat
    85621 gggtgctgaa gtattccacg cattagcttc aatcttgaaa gctcgcggat tagcaacttc
    85681 tgttggtgac gaaggtggat ttgctccaaa ccttggttca aacgaagaag gttttgaagt
    85741 aatcatcgaa gcaatcgaaa aagctggcta tgtacctggt aaagacgttg ttcttgctat
    85801 ggatgctgct tcttcagaat tctacgacaa agaaaaaggt gtttacgtac tagctgactc
    85861 aggcgaaggc gaaaaaacaa ctgacgaaat gatcaaattc tacgaagaat tagtttctaa
    85921 atatccaatc atctcaatcg aagatggatt agacgaaaac gactgggacg gattcaagaa
    85981 attaactgac gtattaggcg acaaagttca attagttggt gacgacttgt tcgtaacaaa
    86041 cactcaaaaa ttatctgaag gtatcgaaaa aggaattgct aactcaatcc ttatcaaagt
    86101 gaaccaaatc ggtacattaa cagaaacttt cgaagctatc gaaatggcta aagaagctgg
    86161 ctacacagca gttgtatctc accgttctgg tgaaacagaa gattcaacaa tctctgatat
    86221 cgctgttgca acaaacgctg gccaaatcaa aactggttct ctatcacgta ctgaccgtat
    86281 tgctaaatac aaccaattac tacgtatcga agaccaactt ggtgaagttg cagaatacaa
    86341 aggtttgaaa tctttctaca acttaaaaaa caaataattc ttttctaatg aagatacatg
    86401 atcgactatt gaaagatagt cgagtatgat cacttagtta agtaatcgaa ctctctactt
    86461 gctgaaatat cagcaggtag agagtttttt tgcaactagc ttctgtatat gatgtttaca
    86521 aagaatggta caagaaaaga tcatccttga aaaaggaaag ctaacaataa ctagcatcat
    86581 ataagagctt ttaaggatag aacagtagaa taggaatcta tgggcaagtc ctctcgtata
    86641 aataaaatag gagggaagta gcagcatatt tttcataaag tggctctctt tcttcatttt
    86701 tactttggtt gctcctattt gaaaattcga gcacactcaa atacaataaa aagaaatggc
    86761 aagggagagg attagatgaa aaaagaagaa aaaatcgcga ttttacaaga gattatccga
    86821 attaaatcag tgaatggaaa cgaaggtgaa gtagcagcct atttgaataa gcttttggca
    86881 aagcatgaca taacaggaga gattgtttct tacagagatg gtagagataa tttgattgct
    86941 cgttaccaaa agggccaatc aggaaaagtg ttaggcctat ctggacatat ggatgtcgtg
    87001 gcggctggtg atgaatcgtc ttggacctat gcaccctttg cagcagaaat ccatgggaac
    87061 cgtttgtacg gacgtggtgc tacagatatg aagagtggct tggcagcaat ggtgattgcc
    87121 atgatcgaac tgaaagagtc aggaaaacca tttaacggaa cagtcaaatt attagccact
    87181 gtcggagaag aagtgggtga actaggtggg gaacagctca cgaaagcagg ctatgtagat
    87241 gacttagatg ccttgatcat cggagagcca accaattaca gtttgatgta tacgcacatg
    87301 ggatcaatca actatacagt cacctcgcac ggaaaagaag cgcatagctc tatgccagac
    87361 caaggttata atgcaatcaa tcacctaaat gaattcatta cgaaagcaaa tgctgaaatg
    87421 aatcacctcg ctgaaacaat cgagaatcct gtcctaggaa aaacaatcca taatgttaca
    87481 ttgatcagtg gcgggaatca agtcaacagc atcccaagcc atgcgcagct ccaagggaac
    87541 attcgatcga ttccagaata cccaaatgat aaaatcattg cattgctgca atcaatcgtc
    87601 aatgaattga atcaagagac agactatcat ttggaattga tgatcgatta caacaaaata
    87661 cctgtcaaag ctgatccaga ttctcctttg attcattgca ttcagcaaca attcagccag
    87721 ccattacctt tagttggagc ggcagcaaca acggatgcag ccgaatttac gaaagctaac
    87781 cattccttcg actttgtcgt ctttgggcct ggtgtcgtta ctttgcctca tcaagtagat
    87841 gagtatgttg aaatcgataa ttatttggat atgatcgaaa aatatcaagg aattatttta
    87901 tcctacttag cgtaagattt ttttaat
//
LOCUS       DAJSBM010000005        80438 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJSBM010000005 DAJSBM010000000
VERSION     DAJSBM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.005"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..235
                     /locus_tag="ONT67_000422"
     CDS             <1..235
                     /locus_tag="ONT67_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2354185.1"
                     /translation="SGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVI
                     TNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            470..706
                     /locus_tag="ONT67_000423"
     CDS             470..706
                     /locus_tag="ONT67_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354186.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            713..2665
                     /locus_tag="ONT67_000424"
     CDS             713..2665
                     /locus_tag="ONT67_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2354187.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT67_000425"
     CDS             complement(2737..3234)
                     /gene="dfrG"
                     /locus_tag="ONT67_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2354188.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3596..4636
                     /locus_tag="ONT67_000426"
     CDS             3596..4636
                     /locus_tag="ONT67_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2354189.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4734..4922
                     /locus_tag="ONT67_000427"
     CDS             4734..4922
                     /locus_tag="ONT67_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2354190.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(4980..5333)
                     /locus_tag="ONT67_000428"
     CDS             complement(4980..5333)
                     /locus_tag="ONT67_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354191.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5864..6286
                     /locus_tag="ONT67_000429"
     CDS             5864..6286
                     /locus_tag="ONT67_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2354192.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6283..6513
                     /locus_tag="ONT67_000430"
     CDS             6283..6513
                     /locus_tag="ONT67_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354193.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7011..7211
                     /locus_tag="ONT67_000431"
     CDS             7011..7211
                     /locus_tag="ONT67_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2354194.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7238..8431
                     /locus_tag="ONT67_000432"
     CDS             7238..8431
                     /locus_tag="ONT67_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2354195.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT67_000433"
     CDS             complement(8499..10064)
                     /gene="guaA"
                     /locus_tag="ONT67_000433"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2354196.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10123..11046)
                     /locus_tag="ONT67_000434"
     CDS             complement(10123..11046)
                     /locus_tag="ONT67_000434"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR2354197.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11242..11847
                     /locus_tag="ONT67_000435"
     CDS             11242..11847
                     /locus_tag="ONT67_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2354198.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12155..13171
                     /locus_tag="ONT67_000436"
     CDS             12155..13171
                     /locus_tag="ONT67_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2354199.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13312..14613
                     /locus_tag="ONT67_000437"
     CDS             13312..14613
                     /locus_tag="ONT67_000437"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCR2354200.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14659..15321
                     /gene="deoC"
                     /locus_tag="ONT67_000438"
     CDS             14659..15321
                     /gene="deoC"
                     /locus_tag="ONT67_000438"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR2354201.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15342..15740
                     /locus_tag="ONT67_000439"
     CDS             15342..15740
                     /locus_tag="ONT67_000439"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR2354202.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15806..16888
                     /locus_tag="ONT67_000440"
     CDS             15806..16888
                     /locus_tag="ONT67_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2354203.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17100..18662
                     /locus_tag="ONT67_000441"
     CDS             17100..18662
                     /locus_tag="ONT67_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354204.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18655..19788
                     /locus_tag="ONT67_000442"
     CDS             18655..19788
                     /locus_tag="ONT67_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354205.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19788..20744
                     /locus_tag="ONT67_000443"
     CDS             19788..20744
                     /locus_tag="ONT67_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354206.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20843..22009
                     /gene="deoB"
                     /locus_tag="ONT67_000444"
     CDS             20843..22009
                     /gene="deoB"
                     /locus_tag="ONT67_000444"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR2354207.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22033..22851
                     /locus_tag="ONT67_000445"
     CDS             22033..22851
                     /locus_tag="ONT67_000445"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2354208.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22868..23575
                     /gene="deoD"
                     /locus_tag="ONT67_000446"
     CDS             22868..23575
                     /gene="deoD"
                     /locus_tag="ONT67_000446"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR2354209.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT67_000447"
     CDS             complement(23873..24559)
                     /gene="gpmA"
                     /locus_tag="ONT67_000447"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2354210.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24731..25312)
                     /locus_tag="ONT67_000448"
     CDS             complement(24731..25312)
                     /locus_tag="ONT67_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2354211.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT67_000449"
     CDS             complement(25445..26128)
                     /gene="rpiA"
                     /locus_tag="ONT67_000449"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR2354212.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT67_000450"
     CDS             26447..26860
                     /gene="rpsL"
                     /locus_tag="ONT67_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR2354213.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT67_000451"
     CDS             26944..27414
                     /gene="rpsG"
                     /locus_tag="ONT67_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR2354214.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27508..29595
                     /gene="fusA"
                     /locus_tag="ONT67_000452"
     CDS             27508..29595
                     /gene="fusA"
                     /locus_tag="ONT67_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2354215.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29767..30954
                     /gene="tuf"
                     /locus_tag="ONT67_000453"
     CDS             29767..30954
                     /gene="tuf"
                     /locus_tag="ONT67_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2354216.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT67_000454"
     CDS             31361..31669
                     /gene="rpsJ"
                     /locus_tag="ONT67_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR2354217.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31700..32329
                     /gene="rplC"
                     /locus_tag="ONT67_000455"
     CDS             31700..32329
                     /gene="rplC"
                     /locus_tag="ONT67_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR2354218.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32357..32980
                     /gene="rplD"
                     /locus_tag="ONT67_000456"
     CDS             32357..32980
                     /gene="rplD"
                     /locus_tag="ONT67_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR2354219.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32980..33270
                     /gene="rplW"
                     /locus_tag="ONT67_000457"
     CDS             32980..33270
                     /gene="rplW"
                     /locus_tag="ONT67_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR2354220.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33309..34142
                     /gene="rplB"
                     /locus_tag="ONT67_000458"
     CDS             33309..34142
                     /gene="rplB"
                     /locus_tag="ONT67_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR2354221.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT67_000459"
     CDS             34185..34463
                     /gene="rpsS"
                     /locus_tag="ONT67_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR2354222.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34486..34833
                     /gene="rplV"
                     /locus_tag="ONT67_000460"
     CDS             34486..34833
                     /gene="rplV"
                     /locus_tag="ONT67_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR2354223.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT67_000461"
     CDS             34847..35503
                     /gene="rpsC"
                     /locus_tag="ONT67_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR2354224.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35506..35940
                     /gene="rplP"
                     /locus_tag="ONT67_000462"
     CDS             35506..35940
                     /gene="rplP"
                     /locus_tag="ONT67_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR2354225.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT67_000463"
     CDS             35930..36118
                     /gene="rpmC"
                     /locus_tag="ONT67_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR2354226.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT67_000464"
     CDS             36143..36409
                     /gene="rpsQ"
                     /locus_tag="ONT67_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR2354227.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36467..36835
                     /gene="rplN"
                     /locus_tag="ONT67_000465"
     CDS             36467..36835
                     /gene="rplN"
                     /locus_tag="ONT67_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR2354228.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36872..37180
                     /gene="rplX"
                     /locus_tag="ONT67_000466"
     CDS             36872..37180
                     /gene="rplX"
                     /locus_tag="ONT67_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR2354229.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            37208..37747
                     /gene="rplE"
                     /locus_tag="ONT67_000467"
     CDS             37208..37747
                     /gene="rplE"
                     /locus_tag="ONT67_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR2354230.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37766..37951
                     /locus_tag="ONT67_000468"
     CDS             37766..37951
                     /locus_tag="ONT67_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCR2354231.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT67_000469"
     CDS             37987..38385
                     /gene="rpsH"
                     /locus_tag="ONT67_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR2354232.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38417..38953
                     /gene="rplF"
                     /locus_tag="ONT67_000470"
     CDS             38417..38953
                     /gene="rplF"
                     /locus_tag="ONT67_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR2354233.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            39114..39470
                     /gene="rplR"
                     /locus_tag="ONT67_000471"
     CDS             39114..39470
                     /gene="rplR"
                     /locus_tag="ONT67_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR2354234.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT67_000472"
     CDS             39491..39991
                     /gene="rpsE"
                     /locus_tag="ONT67_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR2354235.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT67_000473"
     CDS             40006..40185
                     /gene="rpmD"
                     /locus_tag="ONT67_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR2354236.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            40229..40669
                     /gene="rplO"
                     /locus_tag="ONT67_000474"
     CDS             40229..40669
                     /gene="rplO"
                     /locus_tag="ONT67_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR2354237.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40669..41964
                     /gene="secY"
                     /locus_tag="ONT67_000475"
     CDS             40669..41964
                     /gene="secY"
                     /locus_tag="ONT67_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR2354238.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            42026..42673
                     /locus_tag="ONT67_000476"
     CDS             42026..42673
                     /locus_tag="ONT67_000476"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR2354239.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42864..43082
                     /gene="infA"
                     /locus_tag="ONT67_000477"
     CDS             42864..43082
                     /gene="infA"
                     /locus_tag="ONT67_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR2354240.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT67_000478"
     CDS             43115..43231
                     /gene="rpmJ"
                     /locus_tag="ONT67_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR2354241.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT67_000479"
     CDS             43249..43614
                     /gene="rpsM"
                     /locus_tag="ONT67_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR2354242.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT67_000480"
     CDS             43642..44031
                     /gene="rpsK"
                     /locus_tag="ONT67_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR2354243.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            44111..45049
                     /locus_tag="ONT67_000481"
     CDS             44111..45049
                     /locus_tag="ONT67_000481"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR2354244.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT67_000482"
     CDS             45077..45457
                     /gene="rplQ"
                     /locus_tag="ONT67_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR2354245.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45729..46568
                     /locus_tag="ONT67_000483"
     CDS             45729..46568
                     /locus_tag="ONT67_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2354246.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46544..47413
                     /locus_tag="ONT67_000484"
     CDS             46544..47413
                     /locus_tag="ONT67_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2354247.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47403..48200
                     /locus_tag="ONT67_000485"
     CDS             47403..48200
                     /locus_tag="ONT67_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2354248.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48403..49149
                     /gene="truA"
                     /locus_tag="ONT67_000486"
     CDS             48403..49149
                     /gene="truA"
                     /locus_tag="ONT67_000486"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2354249.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49482..52151)
                     /locus_tag="ONT67_000487"
     CDS             complement(49482..52151)
                     /locus_tag="ONT67_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCR2354250.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52389..52577)
                     /locus_tag="ONT67_000488"
     CDS             complement(52389..52577)
                     /locus_tag="ONT67_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR2354251.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52847..53209
                     /locus_tag="ONT67_000489"
     CDS             52847..53209
                     /locus_tag="ONT67_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2354252.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53261..54943
                     /locus_tag="ONT67_000490"
     CDS             53261..54943
                     /locus_tag="ONT67_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCR2354253.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            55060..57096
                     /gene="recG"
                     /locus_tag="ONT67_000491"
     CDS             55060..57096
                     /gene="recG"
                     /locus_tag="ONT67_000491"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR2354254.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            57145..58146
                     /gene="plsX"
                     /locus_tag="ONT67_000492"
     CDS             57145..58146
                     /gene="plsX"
                     /locus_tag="ONT67_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR2354255.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58268..58510
                     /gene="acpP"
                     /locus_tag="ONT67_000493"
     CDS             58268..58510
                     /gene="acpP"
                     /locus_tag="ONT67_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2354256.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58778..59782
                     /locus_tag="ONT67_000494"
     CDS             58778..59782
                     /locus_tag="ONT67_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354257.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59783..60727
                     /locus_tag="ONT67_000495"
     CDS             59783..60727
                     /locus_tag="ONT67_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354258.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60727..61689
                     /locus_tag="ONT67_000496"
     CDS             60727..61689
                     /locus_tag="ONT67_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354259.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61716..62630
                     /locus_tag="ONT67_000497"
     CDS             61716..62630
                     /locus_tag="ONT67_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354260.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62656..64437
                     /locus_tag="ONT67_000498"
     CDS             62656..64437
                     /locus_tag="ONT67_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2354261.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64785..65471
                     /locus_tag="ONT67_000499"
     CDS             64785..65471
                     /locus_tag="ONT67_000499"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR2354262.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65491..69072
                     /gene="smc"
                     /locus_tag="ONT67_000500"
     CDS             65491..69072
                     /gene="smc"
                     /locus_tag="ONT67_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCR2354263.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            69081..69890
                     /locus_tag="ONT67_000501"
     CDS             69081..69890
                     /locus_tag="ONT67_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2354264.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT67_000502"
     CDS             69901..70899
                     /gene="ftsY"
                     /locus_tag="ONT67_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR2354265.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT67_000503"
     CDS             71086..73353
                     /gene="gshAB"
                     /locus_tag="ONT67_000503"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCR2354266.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73442..73894)
                     /locus_tag="ONT67_000504"
     CDS             complement(73442..73894)
                     /locus_tag="ONT67_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCR2354267.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            74044..74994
                     /locus_tag="ONT67_000505"
     CDS             74044..74994
                     /locus_tag="ONT67_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2354268.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74987..75946
                     /locus_tag="ONT67_000506"
     CDS             74987..75946
                     /locus_tag="ONT67_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCR2354269.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75943..76698
                     /locus_tag="ONT67_000507"
     CDS             75943..76698
                     /locus_tag="ONT67_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2354270.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76721..77674
                     /locus_tag="ONT67_000508"
     CDS             76721..77674
                     /locus_tag="ONT67_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2354271.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78318..78614)
                     /locus_tag="ONT67_000509"
     CDS             complement(78318..78614)
                     /locus_tag="ONT67_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCR2354272.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78969..79325
                     /locus_tag="ONT67_000510"
     CDS             78969..79325
                     /locus_tag="ONT67_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2354273.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79335..80234
                     /locus_tag="ONT67_000511"
     CDS             79335..80234
                     /locus_tag="ONT67_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR2354274.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        26318 a        14464 c        17098 g        22558 t
ORIGIN      
        1 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
       61 ttgttctctg ttccctcttt atcatggaag tgcaaaaagt aatataggga ttgataacct
      121 tatagaagtt attactaata aattttattc atcaacacat cgaggtccgt ctgaactttg
      181 cggaaatgtt ttcaaaattg aatatacaaa aaaaagacaa cgtcttgcat attaagtagt
      241 tcagttttgg agtacaaaaa gtgaaaatgt aaacctaaat gagtatcaaa ttgcaactga
      301 atttgatact cgttttttat tgttaaatca acgtttgttg ttaatttaca ccacaaaatg
      361 taagttaaaa tgtatataaa aatgcgaacg tgaaataatt attgtaggaa tgcaaattgg
      421 tgtgttaaaa tggttacaat ttactaagca aaaaggggtg cattttccta tgcaatttct
      481 tgagccaaaa aataagaacg ctaagtctgt ggattgggaa atatcagagc aagtaagggt
      541 gatagttaag caatacgctg aatatgcaga gcgtactgaa agcgaagcag tggatgaatt
      601 cttattgaac attttagacg ataagaagtt tattgaatgg attgccaaca agcgaagtaa
      661 taagcggatt gttgaaaaaa tgggtatcaa agatagagtg ggttgataga caatgaccaa
      721 gcttaaaaga ttagccacga aggaagatga aattgtagat gttaaaattc cgatttcaaa
      781 cgaagaatta aaagataggg ctaaacaata tgatttatta actccaaaac gatttgcaac
      841 aaggtataac aaaatgttgt tcttgccaac gtcttttaaa tggaacggtt ctgaatatcc
      901 cattcaatat aattactgca tcaatccgtt ttgttgtaat tttgggaaag aacagcacaa
      961 attcaaagat gtaaaaggga aaccaagtcg atataaaatg actggtagtt ccaaagataa
     1021 aggtcataaa ggtatgtact gcaatgacaa tcctattggc agaggtgtat cgcaaaactg
     1081 tacagtaacc cctttatcta attggtctgt tgttgaagaa attaaacgcc ttatagaaat
     1141 caatagcatc caagatgttg agccagatta tcaatttcat aaagaaggct gctcggaaga
     1201 agaatcaacc ccattcaatg agccgaagca attttacaaa agggggaaaa gtaggggcaa
     1261 atctcaacgc tatcaatgta aggcgtgtaa gaagttcact aatgttctac caaaaagaga
     1321 agaaaccact acttatcacc aacaaaagaa caccattctt ccaatgttcg ctaagatggt
     1381 tgttggaagg gtttcagtca gtcgtacttg tgatatttta ggaattggag ttggaaccta
     1441 ctatcataag ctggagtggc tgtataggcg ttgtttggag tttttagaac gatatgagac
     1501 acaacccctt caaacaaaga aatttaacga gatgtggctt aatacggaca agatgcacta
     1561 ctacctaaat aacgtccgta agaaaggaca aggttcaaag aaatatacgg ggtttgaaga
     1621 ccttaatatg caaacgtata tagttgtatc tgctgaggtc ttatcaagat atgtgttccg
     1681 ttctgatgtt gcctatgatt ggaatatttc aatggatgaa ttaaatgaag atacaagaaa
     1741 attcaaagaa gaccatctca atacttttag tcgtaaaaat gataggttag attggtctta
     1801 ttatccacaa gaaccatccg ctaacgatag cgaaaatagg aatgcttatc ttcacgaatt
     1861 agggaaaatt actaatagga gtagatttgt cgatggttta aatgttgatg ctccttatac
     1921 tacaactgct cactattggt taattaaaca aatggttaat gccgacgaat ggcgaatgat
     1981 tagtgatgat gacttctcta ttagaaacgc cttttataga gtattcacga aagaattaag
     2041 gttgagcgat gcacatcatt ttatttgcca agttaacaag acgaaaagca gaaaacaatg
     2101 cttaaaagaa ttcggacaag caaaagcaga actgctcgat tggggagata tcagaggatt
     2161 caaaacaaag tttctacgga ctttggcatc tcattatcta actgaattac taacaagtca
     2221 tcaattccac gaagaggcta ttagtaaaga tggagaaaga tatcgcaaat atgccgataa
     2281 ccctatcaaa catccattag ccactaagga taaaggattt tattcagttg attgtagaac
     2341 ggatttatct gctctggaac caaacgaaat cgcaaagatg ctattaaatg ttaatgacca
     2401 ttcgaccaat agctttattc aacagataag acgttatata tcatctcttg aaagaccatt
     2461 aacaacagct cgtggtgata agaagagcta tatttacgct aacttcaatc cgaagtatgc
     2521 tcaatttgcg attacaattc taagaactta ctacaacttt tgtcgccctt ttaaatcagc
     2581 ggataaaaag gttttgacac ctgctcaaag attagggatt acagataaac aatttgattg
     2641 gaaagatata atttatttca aatgacaaaa agagcttgga gtaatcccaa gctcttgtgc
     2701 tttatcttta ctgttcttct tcaattaacg cacccgttaa ctcaataagt tttttctttc
     2761 atatacatga aaatagtagt tatacggatt tttatcattc tttatccctt tttgggcaaa
     2821 tacctcattc cattcctcat aattcacttc tggaaaaaaa gtatctcctt cgaattcatg
     2881 atgtattttt gtgatgtaca ttttctcaac ataagggaaa aacaaattat aaatctgttc
     2941 tcctccgaaa ataaaaattt cttcttcgtt tttacataac tcaaaaacat cttctattga
     3001 atgaacaatt tcacaaccat taaaggtaaa ccccttatct ctcgtcagaa taatatttct
     3061 tctgtcaggt aaggctcttc cgattgattc aaggttcttc ctacctaata ttatcggatg
     3121 tccctttgta gtatttttaa catattccca gtccttggga atcctccaag gaatgtcatt
     3181 ctctttgcca atcactctat tcttatccat cgcagcaatc aaagaaactt tcatttataa
     3241 cacagccttt ttaactaatt taccatctaa tttcttattt ctatcaaaaa accttttctc
     3301 attatcctgc ccgttagttg aataagcaat tttttctata agttatatta tcataatacc
     3361 acaaaatcac aaattttcat tcaattatca ttttcaattg cattttataa atcattttgt
     3421 atgtaatttg gtatgtaagt taaaatgaaa aaagtagtcc attttgaagt acattttgtc
     3481 attttgtact ccaaaactga actacttact tgcatatata cgcctttata gtggagtact
     3541 acatttacga gattcggtta gagtatcaga aaaagaaaaa ataaaagtta cagaaatgta
     3601 tacttcaata aatggtgaat tatgtaagat tgatagagct tattctggag aaattgttat
     3661 tttgcaaaat gagtttttga agttaaatag tgttcttgga gatacaaaac tattgccaca
     3721 gagaaaaaag attgaaaatc cgcaccctct actacaaaca actgttgaac cgagtaaacc
     3781 tgaacagaga gaaatgttgc ttgatgccct tttggaaatc tcagatagtg atccgcttct
     3841 acgatattac gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca
     3901 aatggaagtg attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga
     3961 gcctacagtc atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga
     4021 agtgccgcca aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg
     4081 aagtggaatg cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa
     4141 tgcagttatg gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac
     4201 ggattgtaaa atctgtttta agtacggttt atactatagc cctgttagta ctccagcaga
     4261 ttttcggatg cttactccta ttgtactgga gcaagccttt agaaaagctg gaacagaatt
     4321 gttagagcca tatcttagtt ttaaagttta tgcaccacag gaatatcttt cacgggcata
     4381 taacgatgct cccaaatatt gtgcaaatat cgtaaatact caactgaaaa ataatgaggt
     4441 cattattatt ggagaaattc ctgctcgatg tattcaagat tatcgcaatg atttaacttt
     4501 ttttacaaat gggcttagtg tttgtttagc agagctaaaa ggatatcagg ttaccactgg
     4561 cgaacctgtt tgccagaccc gtcgtctaaa tagtcggata gataaagtaa gatatatgtt
     4621 caataaaata acttagtgcg ttttatgttg ttatataaat atggtttctt attaaataag
     4681 atgaaatatt ctttaatata gatttgaatt aaagtggaaa ggaggagatt gttattataa
     4741 actacaagtg gatattgtgt cctatttgtg gaaataaaac aagactacga atacgagtgg
     4801 atactatact taaaaatttc cctttatact gccccaaatg taagaacgaa actttaatta
     4861 atgttcaaaa aatgaatata ataacaatca aagagccaga cgccaagacg cagagccgat
     4921 aatttgagaa atgaaactct catcttatcg gctctttttg tttatctgaa ttttactgac
     4981 tagccttcaa tatttcttgc ttcattgata ccttttgcta gtgcttccat taaggatagt
     5041 tctttgtctg taaagctatc catgtatttt tctacctgta accttcgagt gcttttaatc
     5101 aaattatctg taggtaagaa aaattcatca atagatacat ggagtaatga tacaagatca
     5161 tacagcactt gtgtacttgg gtgttgtcct ttgttttcta tatttgttaa ataacgtggg
     5221 tcaatttcaa tcattgttcc cacttgttca cgagtgagac cccgtttcat tcgagcctct
     5281 ttaatcgcta aaccaacggc tctaaaatcg tatttatctt cttttttacg catactaaat
     5341 cacctctata cattttactg ttcctatata accataaaca ggtatagaaa aacatgttat
     5401 atagtttatg agttcctata gaacactgtg gaatttatta atgcgatgaa tccaatttca
     5461 aatagaaagg tcgctgattt tatttaccta tacaacttta taaaatttag tcatactcct
     5521 agaaagtaga taaaattggc gacttaatgg agggaaaagt cgccatgaga aagataatct
     5581 gtcatctagt tctcttaatt aagttcttat caaaaaaact tatccacctg tactggatag
     5641 atatgaccat caatgtggtt ttgcagatta catagataga aaaatcaaag catatgaaca
     5701 gagaaaacaa tctgtttatg tgcttttttg gtcacttaat aaatatcttt taggatttct
     5761 cttcatttat gcaacaaatc ccccgttcgt gtcggggttt agtatgaagg gagataaaga
     5821 agcacatgcc actccctttc acgacagaaa gggggtgaaa agcatgaaac catcttcttt
     5881 tcaagaaacc atagaaaatc agtttgacta tatctgcaaa caagttattg aagatgaacg
     5941 aaaagattac ttcaaacatc tagacagact atcagcaaaa gaagtctcgt tttctgatat
     6001 agataactac cttgtcaact ctttttctac tgttgatacg tacgtcactg atttccaact
     6061 atttaaatta tttggattaa gaatcaatgt tgaaagtgat ttattaagtg aagccctgcg
     6121 tcatctttca gagaaaaaac ggaacattat tttacttcat tactttatgg atatgagtga
     6181 tgtggagatt gcggagttac taaatttgaa tcgttcgact gtttatcgtc atcgaataag
     6241 tggactagcc atgattaaag aattcatgaa ggagtgtaaa gaatgaaaac agcttatcca
     6301 agggttccct tccccctcat tgtaaaggcg acagatggcg acgtggaagc aattaatcag
     6361 attgttaaac attatcgagg gtatacctca aaacgttcat tacgtcgcat gacagacgaa
     6421 tatggaaatt ctcatatggt tattgatgaa accctacgtg gcagaatgga aacgaaactc
     6481 atctctaaga ttttatcgtt cgaaattaaa tagtcccatt ttttcgtgga agcgtgtcga
     6541 atcgtatcca cgcttcccga tttgggaggt ttgttattcc atcaaagcat attgtgtgtt
     6601 cagtaggttt tgataggcta acaagccaaa cgttctttga caaccaaata aaggcaatcg
     6661 aatacgtttc ggtaagaaaa gagctgatga actaacacgc catgacctac aaaattagtg
     6721 agcgattcat gttagtaatt cgaaagtaat ctttagcagg attgctgcca tgactttctt
     6781 atcgtgataa tgatactccc gtatagccat agtccgagcg ttagaagcgt cacaggcaat
     6841 gggtacggct acatgagaac catgcagaag tgaaattcct gtgagctttg ctagagctgt
     6901 tcgattgcta agatattctt ctcttgtcag tgtattatta taaaactatt tgatataatt
     6961 ataggtgaat attggagttt tcctagccta taattgggaa aggagtaata atgataacgg
     7021 tcacaaaaaa ggatttagtt gagctaggat atggaacttc tttttcagca gacatcataa
     7081 aaaaagcaaa agaattgatg attacaaaag ggcatatcta ctatcaatct cgaaagctag
     7141 atagagtccc tcgtgaagct gttgaagaaa ttctaggaat tcggttttca aatgaaaaca
     7201 ggtctgagta gtttttgcac agtaaggagt aaaaactatg gttaaagacc caataaaaaa
     7261 agcaaagaat ggaacctatt attttcgtgc aaatctaggt tatgattcta aagggaaaaa
     7321 aattcaaaaa tatcgtagtg gattcactac taaaaaagaa gcaagagaag aatattcgaa
     7381 actgctattg atgaaaccag aagaattaag tgagaataag gataaaatga cgtttaaaca
     7441 ttatatcttt gaaattttcc ttccatggta taagacacag gtaaagctca gaacatatga
     7501 aaatcgctta ccaactatta agaagcattt ctcttatttt gataaaatgg ctgtttccga
     7561 tattgagcct attgatgtgc aaaattggca actaaaatta tcaaagaagt gtaagtcttc
     7621 ttatgtaaga gctgttcaag gactattttc agttgctatg gatcgagcaa ttgtccttgg
     7681 gataaccacc acaaatccat ctaagattat tggcaatgtg aaaaagcaaa agtctaaaat
     7741 tgaattttgg acaaaagaag aatttgaaaa agtaatctct ctaatttaca aagaagatta
     7801 ctatcaacac tttttattta tctctctgtg gtttttattt atgactggaa tgcgaattgg
     7861 tgaagctact gccattcaat gggaagatat tgattttgac tcaggagtac taaccatcaa
     7921 caaaacactc tattacaaga atcaatcgaa ttatagattt gttgaaccaa aaacaaaggc
     7981 gagcgttcgt catattgtat tagataaatg cacattaact tatttatccg aatggaagga
     8041 agtacaacaa aatctaattc agacagactt tgtaatgagc tataacggta ttcctaccca
     8101 aaaacataca ctatcatatg ctattgaacg ctatgctaag ctggctggta tccatcgaat
     8161 cagaatccat gggttacggc attctcatgc ttccttgtta atcagtatgg gagaaaatcc
     8221 acttatcatt aaagatcgtc taggacacga agatattgag acgactttag gaacgtatgg
     8281 acatctttat cctaacagta atttcgaggt agctaacaaa ttaaatggag ccatttcctt
     8341 taaagaagca aaacaaaatt atgatacttc accaaagaat caatttactg tagactactt
     8401 gcgaaacaaa gaaatgaaaa aagtgcaata aaagtgcagt aaaaagataa gaagagccag
     8461 aaacccttgg tataagagga tttctagctc tcctacaact attcccactc cactgttgct
     8521 ggtggtttag aagtgatgtc atacacgata cggttgacgt gggctacttc gtttacgata
     8581 cgtacagaga ttttttgcaa gacttcccat gggatacggg caaagtcagc ggtcattccg
     8641 tcgattgaag ttacagcacg gataccgact gtgtagtcgt aagtacgtcc gtcacccata
     8701 acaccgacag agcggatccc tggaagaaca gtgaaatatt gccagatatc acggtcaagt
     8761 ccagcagctg cgatttcttc acgcaagata gcatcagatt cgcgtacgat ttctagcttg
     8821 tcttcagtga tttcgccaag aacacgaata ccaagaccag gaccagggaa tggctgacgc
     8881 caaacgattg aatcaggcat gcctaactga gtccctaatt cacggacttc gtctttgaat
     8941 aatgtgttta atggttcgat caattcaaat tgcatatctt ctggcagtcc acctacatta
     9001 tggtgagatt tgatcgtctg tgctgtttct gtaccagatt cgataacgtc agtatacaac
     9061 gttccttgtg ctaggaaaga gacgccttct tcaccagcaa gttttgttgc ttcgtcatca
     9121 aaaacatata cgaattcatt cccaatgatt ttacgttttt gttctgggtc agatacacct
     9181 ttcaacttgt ctaagaagcg atctcttgca tcgactttga tgatgttcaa gccgaatttt
     9241 ccgcctagac tttccattac ttgttccgct tcgcctttgc gtagcagacc gtggtcaaca
     9301 aagatagatg tcaattggtc accgatcgct ttttgtaaaa gaaccccaac gacactagaa
     9361 tcaacgccac ctgaaagacc aagcaagact tttttgtcac caacttgttc acggattttt
     9421 tctacttgca tatcgataaa gttatccatt gtccagttac cttcacagtg acaaacatca
     9481 aaggcaaagt ggcgcagcaa ttcgattccg tattcggaat gacgtacttc tgcatggaat
     9541 tggataccat aaaaattacg tgttgtgtct tggatagaag caatcggaca atctttgctt
     9601 gttccgactg tttcaaagcc ttcaggaact ttagttacca agtcaccatg gctcatccat
     9661 actgtttgtt gttttggtgt ttctttaaac aagacagctt gatcgcttgt tacttctaac
     9721 aaggctttcc catattcgcg gtttttagca ggctctacgg aaccgccaag tttgtacgtg
     9781 atcaattgca taccatagca aatccctaat acaggaatac ctaaattgaa gatctcagga
     9841 tcgatcccaa atgcattctc atcatataca ctgtttggtc cgccagaaag aatgattccc
     9901 tttggattca tcgcttttac ttcttctgca gtaatgcggt ggctcaataa ttcggaaaag
     9961 actccaagat cacgaatacg tcttgtgatc aattggttat actgactacc gtagtctaat
    10021 acgataatct tttcaacgtt cgtcatatcg gcaacgtttg tcaaatttat tacccctatc
    10081 cctttaaatt ttatctcaaa atagtcaaac tattttaaaa tgctaatatt ttcgcatgaa
    10141 aatcgaatcg atgatatgat tttcagtttt atggagaatt atatcggctc ttcctcgtgt
    10201 aggcaagata tattcctgta aatttttcaa attcacattt ttccatacat cttttgccat
    10261 tttaaaggct tcttcacgat ctccgatagc aaaacgataa taataattct ctggattttg
    10321 gaaagccgta tccagtaacg caccaaaacg atccaaatac catttttcga tcagcacggg
    10381 atcggcatcg acatagacag aaaaatcaaa aaagtcgctg acgtagattt gctgattcgc
    10441 aggtaattga agcgtattga tcccctcaac gatcaagata tcaggctgct ggatcaattc
    10501 gtacttgtct ggaatgatat catacacatc atgtgagtaa acaggtactt ttatctcttc
    10561 ctgaccagat tttacacggt tcaagaaatc gatcagcatc tccatatcat aactttcagg
    10621 aaatccttta cggtccatga ttccacgctc ttttaatata cgatttggat aaagaaatcc
    10681 gtcagtcgtg atcagctgaa cgtttcttcg tttgaaggta cgagacaaaa tcgtctgcag
    10741 cagtctggct gttgtacttt ttccaacggc tacacttccg gcgatcccga taataaaagg
    10801 cggtacttct acgtatttat gaagaaataa acctttactc aagaacaaag attcaaattc
    10861 tttcatataa agataaatca gatgagcaag aggtacatag atatctcgaa catcctgcat
    10921 ggaaatgcgg tcattcaaac ttttgatatt atctagttca tcttgtgtca aaggtgcttg
    10981 cccattacga taaaattctc gccattcttc tcttgccaca cggtagtagt tcattggatc
    11041 attcatatta gcctcctgcg ttctcccatc aatggaacta ttttaacaca aaataaacgc
    11101 aaaccccacc acttaagaga gattgccgat ctttttgtat aatctttata atagaaagaa
    11161 acggagtctt attcttgact tgcgcacgga tacataaaag gatagactgt tgtcatgaaa
    11221 gaaatgagaa ggatggaaga catgacaaat cattactatt ctgaacgccc agatacggcg
    11281 cacgattttg aacaatgggc ttttgagtta aaaggcagaa cctttcaatt tgtaacagat
    11341 tctggtgtat tttcacgtga aacagtggat tatggttcac gtgtcctgat tgatgctttc
    11401 gattgggaag agttacctga tgaaggaaag atcttagatg tgggatgcgg gtatggaccg
    11461 attgggttag ctttggcatt tgcgacacag cggtttgttg aaatggtcga cattaatgaa
    11521 cgagcggtaa gtctagcgca aggaaacgca aagcgtaacc aaatcgaaca tgttgatatc
    11581 catcaatcga atatctatga agcagttcat gaagaaacct atgcagctat cgtaagcaat
    11641 ccgccgatcc gtgctggaaa aaaagttgta catgaaattt tgacaggcgc atatcctcgg
    11701 ttgaagaagg gcggtacact gacaattgtg atccagaaga aacaaggagc gccgagtgcg
    11761 caaaagaaaa tggaagaaac atttggtaat gcagaaatcg tcacaaaaga taaaggatat
    11821 tatatcatca aaagtgtgaa agaataacta gctgaaaaat agtggagcga cgaaagagtg
    11881 gaactgggcg tgcatgtacg ggtacgtatc agttcttttt ttgttgagaa gtttttaaaa
    11941 acaaagaact attttttctt caaaaagaaa tagtaggaac agagtcgttg ttttttacga
    12001 taaaataaat aagatacaga acttgtatag aaggctgcca agtcggtaga taaaggaggt
    12061 ttttatcaaa aaacatactt tccttttttc tctattcttt tcttgggggt tttggtacac
    12121 tgatagggaa tttagataga agggagccaa taggatgaag ctgaccatca gagatatcgc
    12181 agaaatggca ggcgtatcag taacaactgt ctcacagatt ttgaataaca aaggaagcag
    12241 atttagtgat aagacaagaa aaaaagtgtt gtcgatcgtg aatgaacatc attataagcc
    12301 cgattatttc gcttcaaatg tgatcaatcg acattcaaag accatcggaa tgatcgtccc
    12361 tgatgtcact gatttcttct tctccaaact gatcgaaggt gtagagagct atttgaatcc
    12421 tttgggctat gtgatcatgt tatgcaattc tcgtcatagc caagaaaatg agatcaagta
    12481 tttacaagag ctttcccacc gctcagtgga cggaatcttg ctggccacac caaatatttt
    12541 acctgagaaa tatgcattag acagcggttt ttatcaaaaa atgccaatca ttttaatcga
    12601 tcgcggattg aatcgtcggg ataacggacg gcttatcgta aaggaatatg agggcgccta
    12661 tcaagctgtt tcgctgttta ttgaaaatgg ccatacgaag atcggcatgc tgaaggaaac
    12721 cactggatac tatcaattag aagaacgttt caacggatat cgtcatgcgc tgaaagatca
    12781 tggcctgcct tttaatggga aatttgttga acatggagag ttgactgtcc aaggtggata
    12841 tactgcatca aaaaaattac tcaaacataa agatatcacg gctatttttt gcgggaatga
    12901 tgccatggcg atcggatgct atcaggcgat cgatgaattg ggcaaaaaaa tcccagaaga
    12961 tatctcagtc ataggattcg atggattaaa gctgtcagaa tacatgattc cgcaattgac
    13021 gacagtgcag cagccaagtt ttgatattgg attttatgct gcacgtttcc tgatcgatac
    13081 gatcgaattt cctcaaagaa aagttccgaa caaagtgttc gaaacaaaat taattattcg
    13141 tgagagcgta aaagtattga caaagggcta actcggcggt atattgctta tgtaagcatt
    13201 tacaaaggct agttagtgct ttttttattt ttagtaaacc agtttactaa agtggtttac
    13261 ctttcctaaa aataatgtat aataccgaat gaagacaagg aggatcaggc aatgcgaatg
    13321 gtggatctga ttgaaaaaaa gagggatgga aacgagctgt cgaaagagga aatcgagtac
    13381 atcgtcacga actatacgaa tggaaagatt ccagattatc aagtcagtgc attattgatg
    13441 gcaatttttt atcaagacat gacaaatgaa gaaatcacga acttaacatt ggcgattgct
    13501 aattctggtg atgtcatcga tttgagttct cttgaaggaa taaaggtaga taagcattcg
    13561 acaggtggcg ttggtgacac gaccacattg attttagccc ccctagtagc tagtgtcgga
    13621 gtgacagtgg ctaaaatgtc tggacgcggt ctaggttaca caggtggtac actagacaag
    13681 ctagaagcaa ttcccggatt tcagatcgag ctttcggatg aagaatttgt acgcattgtt
    13741 aatgaaagta aagtagcggt aattggtcaa tcaggaaact tggcaccggc ggacaaaaaa
    13801 ttgtatgcct tgcgtgacgt cacagcgacc gttgattctc ttccgctgat tgccagttcg
    13861 attatgagta aaaaaatcgc ggcaggagca gatgcaatcg ttttggatgt gaccactgga
    13921 gacggtgctt tcatgaagaa catcgaagat gcaagacgtc tggcaaagac catgacgagc
    13981 attgggaaac tggctaaccg agaaacagtg gcggttatct cagatatgtc agaacctctt
    14041 ggcgaagcta tcggaaatag tctagaagtt gtcgaggcaa tcgaaacatt acaaggaaat
    14101 ggtccagaag atctagtaga aatgtgctac gccttaggaa gccaaatggt tgttcttgct
    14161 ggcaaagcta agacaaccga tgaagcaaga acgttgcttc aagaagctct ggaatcaggt
    14221 aaagcgttag caaaattcaa agaaatgatc caaaatcaag ggggagaccc agcgatcgtg
    14281 gaacatccag aacgaatttt gactgcacgc tacacaatgg agcttcctgc taaacaaagc
    14341 ggagttgtgt caaaaatcgt ggcaaatgaa ttaggaattg ctgcaatgat gctaggtgct
    14401 gggcgtaaaa ccaaagaaga agatattgat cacgctgttg gattgaaact gcataaaaaa
    14461 attggtgaca ctgtgacaaa aggtgagtca ctattgacga tctatagtaa cgacgaagaa
    14521 atcacttctg tcattgaatt attatataaa aatattgaaa ttggtgaatc ggcaatgaag
    14581 ccaacgttga ttcatgacat catcacagaa taaaggaaaa aagcggactt taccgtttta
    14641 aagaaaaggg gaaaagaaat ggaactaaac cgaatgatcg accatactat tttgaaagca
    14701 gacgcatcta aagaagctgt gatgcaaatt atcgaagaag caaaaaaata ccatttttat
    14761 tctgtctgca tcaatccagc atgggttgcg ctagcgaaag aacaattaca aggtgagcca
    14821 gtagccgtat gtacagtaat cgggtttcct ctaggagcaa acacacccga gacaaaagca
    14881 tttgaaacaa cagatgccat caataacggt gcagatgaag tcgatatggt catcaatatc
    14941 ggtgctttga aatcaaagga tgatcaacaa gtccaaaaag atatcgaagc agttgttgaa
    15001 gcagcaaaag atcgtgcgct tgtcaaagtt attattgaaa catcgttatt agatagagaa
    15061 gaaatcatcc gagcttgcga aatagcaaaa gctgcaggcg ctgattttgt caaaacatca
    15121 actggtttct caacaggcgg agcaaaggta gaggatgtcc gtttgatgcg tgaaactgta
    15181 ggaccagaaa tgggtgtcaa agcttccggc ggtatccata acgaagaaga agcaatggca
    15241 atgatcgaag ctggtgctac aagaatcgga acaagcgcag gagtagcaat cgtatctggt
    15301 tcaactggtg aaggctatta attttttcaa aggagaagct catggcagaa ctcaaacaag
    15361 aatggatcaa cacagctatt gaagcgttag ataaagccta tgttccttat tcccattttc
    15421 cggtaggcgc ttgtctcgta acggaatcag gcaagattta tcaaggcatc aacattgaga
    15481 atgcatcatt tggattaacg aattgtgccg agcgcacagc ttttttcaaa gcagtatctg
    15541 aaggagaaaa aacatttacg catctagtcg tagcggggca cacccccgat cctatctctc
    15601 cttgtggtgc atgtcgacaa gtaatggcag aattctgttc ccctgatatg ccggttacgc
    15661 tagtaggaga caatggtgtg acgaaggcaa cgaccgttcg tgcactgctg ccttatgcat
    15721 ttactgaaaa agatctataa ctcatgttca ttcagtcagc tgactgttga aaaataaaac
    15781 aattatttta ggaggcttta cagaaatgaa aaaagcaaaa ttatttggtt taggtgctgt
    15841 cgcactagca gcagggttat ttttaggtgc atgcggcaac aacggctcta ctgattcatc
    15901 cggtggcaaa agctcatcag acacaacaac tgcagcattg atcacagaca caggtggtgt
    15961 agacgaccgt tcattcaacc agtcagcttg ggaaggacta gaaaaatggg ggaaagatca
    16021 aggactttca cgaggaaatg atggcttcca atatttccaa tcttcaaatg aatcagatta
    16081 catcccaaat attgaccaag cattgaatgc tggattcaaa acgatttttg gtattggtta
    16141 taaactaaaa ccagcgatcc aagaacaagc aacaaacaat acaggtacga actttgttat
    16201 tatcgatgat gtcatcgatg gattagataa cgttgtatct gcaactttca aagacaacga
    16261 agcttcttat cttgcaggtg ttgcagcagc ttatacgact gaaacaaacg tagtcggctt
    16321 tatcggcggt gtaaaaggtg aagtaatcga tcgttttgat gctggattca aagccggtgt
    16381 tgatgcaggt gcgaaagaat taggaaaaga aatcaaagta ttgaatcaat atgctggtga
    16441 cttctctgct ccagataaag gacgttcaat tgctcaaggt atgtatgcac aaaacgcaga
    16501 tatcatcttc catgcttcag gcggtacagg taacggtgta ttccaagaag caaaatcatt
    16561 gaacgaatca ggcgacaaaa aagtttgggt tatcggtgtt gaccgcgacc aatcagacga
    16621 aggcgaatac acattaaacg gcgaaaagaa aaacttcaca ttgacatcta cactgaaagc
    16681 agttggaaca gtagtagaag atttggcaca aaaatcagcg gacggtaaat tccctggcgg
    16741 cgaacacact gtttatggat tgaaagaaga cggtgttggt ctaacagaag gacaactttc
    16801 tgacgaagcg aaaaaagcag ttgatgaagc aaaagaaaaa atcatttcag gtgacgtaaa
    16861 agttccagaa actcctgaag aaaactaaaa ccatttctat agccttgtta gacacatgtt
    16921 tgacaaggct atggtttgat tagaacaaga attgttcatt tatccaagct gccttgtgac
    16981 agtttggata agggaacaac tccctgaagt aatgtttgtt tcaaaagaag aacgctcact
    17041 aagcgtgaat cttagtcaat cgttgcccta gatagaaatc gttaggatgt gaaaaaaagg
    17101 tggttcaaga aaatattgtc atagaaatgc agaatattac caaagaattt ggaacattca
    17161 aggcaaatga caacatcaat ttacaagtaa aggcaggaga aattcatgcg ttgcttggag
    17221 aaaacggtgc tggcaaatct acattgatga acgtgctttc cggattatta gagccgacat
    17281 cagggaaaat tttgatgcgt gggaaagaag taaagatcac aagtccgacc aaagccaacc
    17341 aattagggat tgggatggtc catcagcact ttatgcttgt tgatgccttt actgtaacag
    17401 aaaacatcgt gttgggaagc gagcctagtc gcgcagggat gcttgaccat aaaaaagcac
    17461 gaaaagagat ccaaaaagtt tctgaacagt atggattgtc agtcaaccca gatgcttacg
    17521 ttcgtgatat ttcagttgga atggaacaac gggtagaaat tttaaaaaca ctttaccgag
    17581 gagcagatgt actgattttt gatgagccga cagctgtatt gacccctcaa gaaattgatg
    17641 aattaatcgt gatcatgaag gaattagtca aagaaggcaa gtcgatcatt ttgattacgc
    17701 ataagttaga tgaaatcaaa gcagtagctg accgctgtac agttatccgc cgtggaaaag
    17761 gaatcggtac agtcaacgtt aaagacgtaa cctcacaaca attagccgat atgatggttg
    17821 gaagaacggt ttcattcaaa acgatgaaaa aagaagcgaa gcctcaagaa gtcgttttgt
    17881 ctattgaaaa tctagtggtg aaagaaaatc gtggattaga agccgtgaaa aacttgaact
    17941 tagaggttcg tgctggcgaa gtacttggta tcgctggaat cgatggaaac gggcagtcgg
    18001 agttgatcca agctttgact ggtttgcgaa aggcagaaag cggacatatc aagctaaaag
    18061 gggaagacat cactaataaa aaaccccgaa aaatcactga acatggtgta ggacacgtgc
    18121 cagaagaccg tcataaatac gggttagtcc tagatatgac attatctgaa aatatcgcac
    18181 tacaaaccta tcatcaaaaa ccttacagta aaaacggcat gctgaattat tcagtaatga
    18241 atgaacatgc cagagaattg atcgaagaat atgatgttcg aacaacgaat gaacttgttc
    18301 ccgcaaaagc tttatcaggc ggaaatcagc aaaaagcaat tatcgctcgt gaagtcgacc
    18361 gagatcctga tctgttgatt gttgcaaatc caactcgtgg actggatgta ggtgcgatcg
    18421 aatttattca taaacgtctg atcgaacaaa gggacaaata caaagcagtg ttactgatca
    18481 gtttcgaatt agaagaaatt ttaaatgttt cggatcgtat tgctgttatc catgaaggag
    18541 aaattgtcgg tatcgttgat ccgaaagaaa catctgaaaa tgaattaggt ctattgatgg
    18601 cgggctatac cttagaagaa gcacgaaaag agttagaaaa gggggctggc gcacatgaat
    18661 gatcgcagcg aaaaattccg taatttatta gtgcctgtac tttcagttgt tttaggattg
    18721 atcttaggcg cgatattgat ggctgctttt ggttttcagc ccatccaagg ttattcttct
    18781 atgctagatg cagcccttgg ttcgcaaaga gggataggtg aaacgttaag gcaagcaacg
    18841 ccgttgattt ttacagcatt aggtttttct gtagctaact cagctgggtt tttcaatatt
    18901 gggctttctg gacaagcact ttgcgggtgg atcgtcagtg tctgggtggc acttgcgttt
    18961 ccagatctgc caaaagttgt tttgcttccg ttatgcgtga tcttaggtgc cttagctggt
    19021 gcgatggcag cggctgttcc aggtttactt cgagcatttt ttggaacaag tgaagtgatc
    19081 gtaacaatca tgatgaacta tatcttatta tacgtaagca catttgcttt acatgaagtg
    19141 atgcctgaaa gctatcgctc aagtttggac tcttccaata tgatcagtca aaatgcctca
    19201 ctgcggttac catggttaac acaagctttt ggcggctctc gcgtaaactc aggattattt
    19261 ttagcaatca tcgcgcttat tttcgtttgg ctgctgatga agaaaacgac tttaggattt
    19321 gaaatccgtt ctgttggatt gaatcctttt gcttctgagt atgcaggaat gagtagtaaa
    19381 cgtacgatcg ttttgtcgat ggtcatttct ggcgctttgg cagggcttgg cggtgtggtt
    19441 gaaggattag gaacgtatca aaacttcttt gttcaaacga cttcgctatc tattggattt
    19501 gacggaatgg ctgtttcctt gcttggttct ggatcagcaa tcggtattct tttatcagcg
    19561 atcttgttca gtattttgaa gatcggtgga cttggcatgc agacaggagc aggtgttccg
    19621 tttgagatcg tcaatgtatc gattgctttg attatcttct ttgtcgggat caacttcctg
    19681 attcgtttcg tcatggctaa agtcttccaa gggaaaaaac aagaagagat cgttgcgaca
    19741 atagaatcga aaccaacgaa tcaaaaccat gaagggggcg aactataatg tcgactatcg
    19801 aattaattgc cttgatcctt acttctacac tggtttattc aacaccgcta attctaacgt
    19861 cactaggcgg aactttttct gaacgaagcg ggatcgtaaa cgtagggcta gagggaatca
    19921 tggtcatggg tgcttttagt tccatcgttt tcaacttgac gatgagtcca tcacttggtt
    19981 ctatgacacc atggatcgct attttagtgg gtggcgtcgt aggtatccta ttttctctga
    20041 ttcacgcagt agcaacagtc agtttgcgtg ctgaccatat tatcagcggg acagtaatta
    20101 atctgatggc acctgcttta ggtgttttct tgatcaaagc atggtatggt aaaggtcaaa
    20161 ctgataacat cacgcagaat ctggggtatt tttctttccc aggattagca gatattccgg
    20221 tcattggacc aattttcttt aaaaatacat tcttacctgc ttattgtgct attttgattg
    20281 ctgtcctagc ttgggtgatc atcttcaaga cgaaattcgg cttgcgcctc cgttcagttg
    20341 gtgaaaatcc tcaagctgcc gacacattag gtatcaatgt atatgctatg cgttattcag
    20401 gcgtactgat ttctggcttg ttaggcggta tgggcggtgc tgtttttgct cagtcgattt
    20461 ccggtaactt ttcagctggt acgatcgttg gacaagggtt catctccatg gctgctatga
    20521 tctttggtaa gtggaatcct ttaggggcaa tgggtgcctc attgttcttt ggttttgctc
    20581 aaagcttgag tatcattggc gcacaattac cagttatttc ttcgattccg ccagtattgc
    20641 tgcaaattgc tccatatttg ctgacaatca ttgtgctggt tgttttctta gggaaagctt
    20701 ctggtccaaa agcaaacggg aagaactata tcaaatcaaa ataatctttt agcgaataaa
    20761 aagggtgggt tacgggaagt gtttttcgtc tcgccctttt tgttttaaag gcaataaaaa
    20821 taaaaagaaa gaaggaataa tcatgtttaa acgcgtacat ctgatcgtca tggattcagt
    20881 aggaattgga gaagcaccag atgccgataa gttcggtgat gttggttctg atacattagg
    20941 acacattgcg aaagaagcag gattgaacgt tccacattta gagcagcttg gtttaggaac
    21001 gatccgtcct cttgaaggtg tggaatcagt gaaagatcat caaggctatg cgacgaaatt
    21061 agaagaagtt tctgtaggga aagatacgat gacaggccac tgggaaatca tgggattgaa
    21121 tatcaaaact ccatttcgtg tgtttcctaa tggattccct gatgatctga tcaaacaaat
    21181 cgaagaattt tctggccgta aagtcgtatg taacaaacct tatagcggaa cagctgttat
    21241 tgacgattac ggtgaacacc aaatgaaaac aggcgattta attgtatata cttctgccga
    21301 tcctgtcctt cagatcgcgg cacatgaaga aatcattcct ttagaagaat tatatcggat
    21361 ttgccaattc gttcgtgata taacaaaaga tgatccgtat atgattggcc gaatcattgc
    21421 acgtccgtac gtgggtgaac caggagactt cacacgaaca agcaatcgcc atgattatgc
    21481 gttgaatcca ttcggacaca cagtccttga ttctttgaaa gaagcaggaa aggacgtgat
    21541 tgctgttgga aagatcaacg atattttcaa cggacaagga atcactgaat ctgtccgcac
    21601 gaaaagcaat atggatggcg ttgaccagct gctgaaagtt atgaaaaacg atttttcagg
    21661 aatcagcttt accaatttag tagattttga tgcattatac ggtcatcgtc gggatgtagt
    21721 cggctatgcg catgcgattg aagaatttga cttacgaatt ccagaattgc ttgaagcgat
    21781 ggaagaagac gacttgctaa tgattacagc cgatcacgga aacgatccga cgtttactgg
    21841 taccgatcat acgcgagaat atgtaccgtt attggtttac agtaaaaaaa tgaaagaaca
    21901 aggggcattg cctcaaggat attattcaga tatttcagca acgattgctg agaactttag
    21961 tgtttcttcg actgaaaatg gcgaaagctt cttgaaatta ttaaaataat ctttttaaat
    22021 aggagaaagc aaatgaccaa gttaagcgag atgttacaag aaacgaccca gtttatcctt
    22081 gataaaggag taaaagaaat tgattttggt ttgattctag gttcaggatt aggtgaatta
    22141 gcagatgaaa tcgaagatgc tgtaatgatt tcttatgatg agatcccgtt ctttcctgtt
    22201 tcgacagtag ttgggcatgc aggaaagcta gtttatgggt ctctggcagg caagaaagtg
    22261 ttagccatgc aagggagatt ccactactat gaaggtcatt ccatgcaaac agtgacctat
    22321 ccagtacgtg tgatggccgg actaggggcc cattcagtca ttgtgactaa tgcatgtggt
    22381 ggtgtcaacg aatcatttgt accaggagat ttgatgctga ttaccgatca tatcaatttt
    22441 actggtgaaa atcctttgat cggtccgaat gaagatgcaa tgggccctag atttccagat
    22501 atgagtcaag cttatacgcc tgactatcaa gcaacagcaa aagcagcagc agaaaaacta
    22561 ggtttggatc ttaaagaagg cgtatatatg ggctattctg gaccaactta tgaaacgcct
    22621 gcggagatcc gaatgtctcg gacaatagga gccgatgcag tagggatgtc gactgttcca
    22681 gaagtcatcg tagcagctca cagcggatta aaagtgttag gaatctcttg tattaccaac
    22741 ttagcagcag gtatgcaagc gaatctgagc catgaagaag ttgtagaaac aactcagaga
    22801 gtcaaacaat cttttaaagc attggttaaa gaaacattga cattattgta ataggaggaa
    22861 caataaaatg agtgtgcata ttgaagcgaa agaaggcgag atcgccgata agattttact
    22921 tccgggagat cctttgcgag ctaaatatat tgcggagact tttttggaag atccagtttg
    22981 ttacaatcag gtacgcggta tgttaggctt taccggcaaa tataaaggag aacgtatttc
    23041 tgttcaagga acaggaatgg gaatgccatc agccaccatc tatgcccatg aattgatcca
    23101 gtcgtatggt gtaaagaaat tgattcgagt aggtacatgc ggtgcattgt caaaagacgt
    23161 ccatgttcgt gatttagtac tagcacaagg tgcagcgacc agttcttcga tgatcgaaaa
    23221 aaatttccaa gcctttcatt ttcctccaat cagtgatttc aatctattac tgaaagcata
    23281 tgagatagct aaagaaaagg gctatacggt tcatgtagga aatgttcttt cggaagattc
    23341 tttctacaaa gatgatttga cagagacgtt ccaattagct gaacttggcg ttttaggagt
    23401 ggaaatggaa gcagcggctc tttattactt aggcgcgaaa taccatgtgc agacattaag
    23461 tttgatgacc gttagcgacc atttgatcac cggtgaagaa acaacagctg cagaacgtca
    23521 atctacgttc aatgaaatga tcgaagtagg attggaaaca gcgatagcag aataagaagg
    23581 aaatgaaaaa gctgccctgc attaagtaat aagaacatcc aagcaaaaat agctcctcat
    23641 gtttttgact tggctggact tattattgca aatgtaagct tttgaacacc gtttaccaag
    23701 aggttgtgaa tctgccgttt aactctgagt cacaaggaac aatttcaaaa aacagctttt
    23761 catgtttgat gaatattgtg acttgtgacc gagaagttgg caggagaaaa ccgtttatac
    23821 gaaaaaaggg actgtgacaa atcgtgtcac agtccctttt ttatagtgtt ttttataggt
    23881 agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt tgacctgttg
    23941 gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta gctagggcgc
    24001 gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat gctggagcaa
    24061 tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt tctccaccag
    24121 gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa ccttcgtcag
    24181 tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc acttgttcgt
    24241 cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca tagtgacgtt
    24301 cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat tctaagatca
    24361 agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca aattcgattc
    24421 cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct ggtgctagat
    24481 ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga ccgtgacgag
    24541 aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat ttctgaaatt
    24601 ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa attagataag
    24661 attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg caactgttcg
    24721 tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg atatatttat
    24781 aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt tctttttttt
    24841 cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt cttccgccga
    24901 tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg atcaatgcgc
    24961 actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt tcgcgcgtat
    25021 cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat tgcgtgagtt
    25081 ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact agtgcatcgc
    25141 cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca ttttgttcca
    25201 cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga tcaacctgag
    25261 ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc atggtgattc
    25321 ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata aagtacgtca
    25381 aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat caagcagttt
    25441 ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag ccgacaacta
    25501 ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt tcatctaaat
    25561 aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg atgtaatgac
    25621 caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt tggaatcttt
    25681 tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca actgggagtg
    25741 ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc gtttcttttg
    25801 agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg atcccttgaa
    25861 aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg acttcatcaa
    25921 tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt gaagtagtaa
    25981 ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct accatataat
    26041 aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat ttcgcggctt
    26101 caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag tttgagcatc
    26161 gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct tttttattcc
    26221 aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca ttcgtgatat
    26281 tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact gtttatcaaa
    26341 gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa agaaccacct
    26401 ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc ctacaattaa
    26461 ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac ctgctttgaa
    26521 caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc aaaaacgtgg
    26581 ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt tacgtaaata
    26641 tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag ggattggtca
    26701 taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag acttaccagg
    26761 ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg accgtaaaca
    26821 aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat taaacaaatg
    26881 aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa ggaggagtta
    26941 cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc tatttataac
    27001 tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg cgggattgca
    27061 gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa cgatccatta
    27121 gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa agcacgtcgt
    27181 gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg cgttactcta
    27241 ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat ggaagaacgc
    27301 ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa aaaacgtgaa
    27361 gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg gtaagattct
    27421 ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt tagcaactag
    27481 atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca aaacactcgt
    27541 aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga acgtatcttg
    27601 tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc acaaatggac
    27661 tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac tacagcacaa
    27721 tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt cacagttgaa
    27781 gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc acaatcaggt
    27841 gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt acctcgtatc
    27901 gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt atcaacttta
    27961 catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc ggaagacgac
    28021 ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa cgatcttgga
    28081 acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga agaatggcgc
    28141 gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa atacttagaa
    28201 ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac agttaacgta
    28261 gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca attactattg
    28321 gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa agggattgac
    28381 cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt ctcagcttta
    28441 gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg tgtgtactct
    28501 ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg tgaacgtgtt
    28561 ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt ttacgctgga
    28621 gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact atgtgatgaa
    28681 aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca agtcgctatt
    28741 gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa actatctgaa
    28801 gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat catcgctggt
    28861 atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt caaagttgaa
    28921 gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg cactcaagcc
    28981 gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt atggattgaa
    29041 tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt cggtggggtt
    29101 gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat ggaaaatggt
    29161 gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg ttcataccat
    29221 gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt acgtgcagca
    29281 gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt tgttatccct
    29341 gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg tgttgaaggt
    29401 atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact tgctgaaatg
    29461 tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt tacaatgaca
    29521 tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa gaaaaacggc
    29581 ggaaaagctg aataatttct taaaaccgga tagtcctgca aagagattat ccaaagagac
    29641 tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat gtggtgtacc
    29701 ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt aggaggacat
    29761 tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat tggtactatc
    29821 ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt actatctaag
    29881 aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga agaacgcgaa
    29941 cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg ccactacgct
    30001 cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg tgctgctcaa
    30061 atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca aactcgtgaa
    30121 cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt gaacaaagta
    30181 gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg tgacctatta
    30241 acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc tttgaaagct
    30301 ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc agttgacgaa
    30361 tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt tgaagacgtg
    30421 ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg acaagttcgc
    30481 gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac aacagttact
    30541 ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa cattggtgct
    30601 ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc taaaccaggt
    30661 acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa agaagaaggt
    30721 ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg tacaactgac
    30781 gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg cgacaacgta
    30841 acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa attctcaatc
    30901 cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc ttaattcact
    30961 taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga aggtcttagg
    31021 atctttgttc cgagcttttt tatttattgc aggatcagga aaaaagacag gagtattata
    31081 aatagaagaa acacgaaata aaagagtcag tattttctaa atgaaacttt atgaaaacaa
    31141 aatgtttttt acaagaaaaa gccttgcttt gtgagtttat atatattata atgacaaagg
    31201 tgcgtttact ttagtgaatt gcacagacgt aatcagaatg atttcgtcgg ctaagaaacg
    31261 agaggttgcg acacgcccgg acgctttgcc acgaacgagc gtgacggaaa ttttcgtgga
    31321 gctatgtcta cttttcaaaa taggcgaagg agggaacaag atggcaaaac aaaaaattcg
    31381 tatccgttta aaagcgtatg aacaccgtat tttagatcaa tcagcggata aaatcgtgga
    31441 aactgcaaaa agaactggag ctagcgtgtc tggtccaatt ccattaccaa ctgaacgcag
    31501 tctttacaca atcatccgtg cgactcataa atacaaagat tcacgcgaac aattcgaaat
    31561 gcgtacacac aaacgtctta tcgacattgt gaacccaaca ccaaaaactg ttgatgcttt
    31621 gatgaagcta gacttaccat ctggtgtaaa catcgaaatc aaattataaa actaaaacaa
    31681 aaataatgga ggtgtactca tgaccaaagg aatcttaggg aaaaaagtgg gaatgacaca
    31741 aatcttcact gaatctggtg aattaattcc agtaactgtt gtagaagcta ctccaaacgt
    31801 agtactacaa gtgaaaacaa tggaaacgga tggctacgaa gctattcaag ttggttacca
    31861 agatatgcgt gaagttttat caaacaaacc tgcgaaaggt catgttgcaa aagcaaacac
    31921 ggctcctaag cgcttcattc gtgaattcaa aaatgttgag ctaggagaat atgaagtagg
    31981 aaaagaaatt acagtagatg ttttccaagc aggagacatt attgatgtaa caggtactac
    32041 gaaaggtaaa ggattccaag gggttatcaa acgccacgga caaagccgcg gaccaatggc
    32101 tcacggttct cgttaccatc gtcgtcctgg gtcaatgggt ccagttgcac ctaaccgtgt
    32161 atttaaaaac aaacgtcttg ctggccgtat gggtggcaac cgcgtaacta tccaaaactt
    32221 ggaagttgta cgtgtcgatg cagaaagaaa tgttatcttg atcaaaggta atgttcctgg
    32281 agctaaaaaa tctttaatta ctatcaaatc agctgtgaaa gctaaataat tgtaggaaga
    32341 aaggaggaac taaggaatgc cgaatgtagc attattcaaa caagatggaa gccaaaacgg
    32401 tgaaatcaca cttaacgaag aaatcttcgg aattgaacct aatgaaagtg ttgtttacga
    32461 tgcaatcgta atgcaacgtg cgtcattaag acaaggaaca cacgcggtta aaaaccgtag
    32521 cgctgttcgc ggcggcggac gtaaaccatg gcgtcaaaaa ggaacaggtc gtgcacgtca
    32581 aggatctatc cgctcaccac aatggcgtgg aggtggcgta gtctttggac caacaccacg
    32641 ttcttacagc tacaaacttc ctaaaaaagt tcgtcgttta gcaatgaaat ctgtattgtc
    32701 agaaaaagtt gctgaaaaca acttggtagc aatcgaaggc ttgaacttcg acgcaccaaa
    32761 aacaaaagaa ttcaaacaag ttcttgctaa cttatctatc gactctaaag tattggttgt
    32821 tctagaaaaa ggtaacgact ttgctgcttt atcagcacgt aacttaccaa acgtttctgt
    32881 agtaacttct gataatgtga gtgtgctaga cgttgtatca aatacaaaag tgttagcaac
    32941 acaaactgct cttactcaaa ttgaggaggt gcttgcataa tgaacttact agacgtaatc
    33001 aaacgcccag tgatcactga aaaatcaatg cttgccatgg atgacaagaa atacactttt
    33061 gaagtggaca ctcgcgcaaa caaaacttta gtaaaacaag cggtagaagc tgcttttgac
    33121 gttaaagtca aaaacgtgaa catcgtgaac gtacgtgcga aattcaaacg tatgggcaaa
    33181 tatgcaggat atacaaaaaa acgtcgcaaa gcgatcgtta cattgactga agattcaaaa
    33241 gaaatccaat tattcgaagc tgctgaataa gcactaatag ataactcatt aaattaggag
    33301 ggaaaaacgt ggcgattaaa aagtacaaac ctacctcaaa tggtcgtcgt aacatgacag
    33361 gttctgattt tgctgaaatc acaacatcaa cacctgaaaa atcattgtta caaccattga
    33421 aaaaccatgc tggacgtaac aacaacggcc gcatcactgt acgtcatcaa ggtggcggac
    33481 acaaacgcca atatcgcgtg atcgacttca aacgtaacaa agataacgtt gttgcaactg
    33541 ttcaaacgat cgaatatgat ccaaaccgtt cagctaacat cgcgttagta cactatgaag
    33601 atggagtgaa agcttacatc ttagcaccta aaggactaga agtgggtatg aaacttgttt
    33661 caggtccaga agcagatatc aaagtcggaa atgctcttcc attagaaaac attccagttg
    33721 gtactgttat ccacaacatt gaaatgaaac ctggtaaagg tggacaattg atccgttctg
    33781 ctggtacaag tgctcaagta cttggtaaag aaggcaaata cgtattgatc cgcttgaact
    33841 ctggtgaagt tcgtatgatc ttagctgcat gccgtgctac aatcggttct gtaggtaacg
    33901 aacaacacga attaatcaac atcggtaaag ctggacgctc tcgttggatg cgtaaacgcc
    33961 caactgtacg tggtagcgta atgaacccta atgatcaccc acacggtggt ggtgaaggta
    34021 aagctcctat cggacgtaaa gcaccagttt ctccttgggg tcaaccagct cttggttaca
    34081 aaactcgtaa caaaaaagct aaatcagaca aacttattgt tcgtcgtcgc aaaaataaat
    34141 aattactttg accatgtgtc gcacattttg agaggaggtt caccatgggt cgtagtttaa
    34201 agaaaggacc ttttgtcgat gagcatttaa tgaaaaaagt tgaagctcaa gcaggggttg
    34261 aaaagaaaaa agttatcaaa acttggtcac gccgttcaac agttttccca agttttgttg
    34321 gatataccat cgcagtttat gatggacgta aacatgtacc agtttacatc caagaagaca
    34381 tggtaggaca caaattaggt gaatttgcac ctacaagaac ttatcgtgga catgctgctg
    34441 acgataagaa aacgaaacgc taatttgagg ggaggataaa tccaaatggc agaacaaatc
    34501 acatcagcta aagcaactgc aaaaacagtt cgcacttcac ctcgaaaagc ccgtttagta
    34561 attgacctta tcagaggtaa aagtgtcgca gatgcaattt caatcttgaa attcacacca
    34621 aacaaatctg ctggaatcat tgaaaaagta ttgatgtcag cagttgcgaa tgcagaaaat
    34681 aactttgact tagatgttga aaacttggta gtaagcgaag cttttgttaa cgaaggacct
    34741 acaatgaaac gtttccgtcc tcgtgcaaaa ggatcagctt ctccaatcaa caaacgtaca
    34801 agtcatatca cggtagtcgt atcagaaaaa taaggaggga taatcagtgg gtcaaaaagt
    34861 acatccaatt ggaatgcgtg taggcatcat ccgcgactgg gatgctaaat ggtatgctga
    34921 aaaagagtat gctgagttct tacacgaaga tttaagaatc cgtaaattta tttcaactaa
    34981 acttgctgat gctgctgtat caacagttga aatcgaacgc gcaacaggtc gcgtaaacat
    35041 ttcaattcac acagctaaac caggtatggt tattggtaaa ggcggttctg aagtcgaaag
    35101 cctaagaaaa gaattaaaca aattaactgg taaaagaatc catatcaata tcgtggaaat
    35161 caaaaaacca gatctagatg caaaattagt aggtgaagga atcgcacgtc aattagaaaa
    35221 ccgtgttgct ttccgtcgcg ctcaaaaaca agccatccaa cgctcaatgc gtgctggcgc
    35281 tcaagggatc aaaactcaag tatctggtcg tctaaacgga gcagatatcg ctcgttcaga
    35341 aggatactca gaaggaactg ttcctcttca tactttacgt gcggacattg attacgcatg
    35401 ggaagaagca gatacaacat acggaaaact aggagttaaa gtgtggatct atcgtggaga
    35461 aattcttcca actaaaaaaa acactgagaa aggagggaaa taatcatgtt agtacctaaa
    35521 cgtgtaaaac accgtcgtga attccgcgga aaaatgcgcg gggaagctaa aggcggaaaa
    35581 gaagtagcat tcggtgaata cggtttgcaa gctgttgatt cacattggat cacaaaccgc
    35641 caaatcgaag ctgctcgtat cgcaatgact cgttacatga aacgtggtgg gaaagtatgg
    35701 attaaaattt tccctcacaa atcttatact gccaaagcaa ttggggtacg tatgggttct
    35761 ggtaaagggg cacctgaagg atgggttgca ccagtaaaac gtggtaaaat catgtttgaa
    35821 atcgcaggcg ttcctgaaga agtagctcgt gaagcgttac gtctagcttc tcacaaatta
    35881 ccaatgaaaa ctaagatcgt aaaacgtgag gaaatgggtg gtgaatcgaa tgaaggttaa
    35941 agaaatcaga gaattaacca ctgccgaaat gcttgatcaa gaaaaacaat taaaagaaga
    36001 attgtttaat cttagattcc aattagcaac aggtcaatta gaaaacactg cacgtattaa
    36061 agaagtacgt aaatcgattg cacgcatcaa aactgtgttg cgtgaacaag ctaagtaatt
    36121 atggaaggag gccattaagc gtatgactga aggaagaaat caacgcaaag tttatcaagg
    36181 tcgcgtggtt tcagacaaaa tggataaaac aattaccgtt gctgtcgaaa caaagaaaaa
    36241 ccaccctatc tatggcaaac gcatgaacta ttctaagaaa tacaaagtgc atgatgaaaa
    36301 caacacagca aaagttggcg acatcgtgag aattatggaa actcgtccat tatcagctac
    36361 aaaacgtttc cgtttgttgg aagtagtcga agaagcagtt attatctaat aacacgagat
    36421 tttcctatat agattgaatg caaaatctga aaggaggata cacaacgtga tccaagcaga
    36481 aagtcgttta aaagtcgctg ataactcagg cgcacgtgaa attttgacga tcaaagtcct
    36541 tggtggatct ggtcgtaaaa ctgctaacat cggtgatgtg atcgttgcta cggttaaaca
    36601 agcaacgcca ggtggggttg tcaaaaaagg tgacgtcgtt aaagccgtta tcgttcgaac
    36661 aaaatcagga gctcgtcgta cagacggttc atatattaaa tttgatgaaa atgctgcagt
    36721 gattattcgt gatgataaaa gccctcgtgg aacacgtatc tttgggccag ttgcacgtga
    36781 actacgtgaa aacaacttca tgaagatcgt ttcactagca ccagaagttt tataatcttg
    36841 agacacttat caaaggaggt gcgaaacagt tatgtttgtt aaaaaaggcg ataaagttaa
    36901 agttatcact ggaaaagaca aaaacaaaga aggcgttgta ctagcagcgt tcccgaaaaa
    36961 agataaagtt attgtcgaag gtgtaaatgt cgttaaaaaa caccaaaaac cttctcaagc
    37021 tgctcctcaa ggtggaattg ttgagatgga agcgccaatt catgtttcta acgtgatggt
    37081 ggttgactct actggtgtag ctggccgagt tggctacaaa gaagtagacg gcaaaaaagt
    37141 ccgtgtttct aaaaaaaccg gtgaagtttt agataaataa aagtttaagg aaggagggtc
    37201 ttactgaatg aaccgcctaa aagaaaaata tcttaaagaa gtaactccat cattgatgga
    37261 aaaatttaac tatagctctg ttatgcaaac acctaaagtt gaaaaaatcg tcatcaacat
    37321 gggtgttggt gatgcggtat caaacgctaa aaacttagat aaagcagttg aagaattaac
    37381 tttgatcact ggtcaaaaac caatgattac aaaagctaaa aaatcaatcg ctggattccg
    37441 tttacgtgaa ggaatgccaa ttggtgcaaa agttacattg cgcggagaaa gaatgtacga
    37501 attcttagac aaattggttt ctgtttcact accacgtgta cgtgacttcc atggtgtaag
    37561 taaaaaagcc tttgacggtc gtggaaacta tactttaggt attaaagaac aattgatctt
    37621 cccagaagta gattatgatt tagtagataa agtacgtggt atggacatcg ttattgtaac
    37681 aacagcaaac tctgatgaag aatctcgtga gttgttgaca caattaggca tgccattcca
    37741 aaaataatta aaggaggcga actacgtggc taaaaaatca atgattgcta aaaacaaacg
    37801 tcctgcaaaa ttttcaacac aagcttatac tcgttgcgaa cgttgcggac gtccacattc
    37861 agtttatcgt aagttccatc tttgccgtat ttgcttccgc gaacttgcct ataaaggtca
    37921 aattcccggc gtgaagaaag ctagctggta aaaagtaaat tcgcataaga aggaggtaaa
    37981 agatcaatgg tcatgacaga tccaattgca gattttctaa ctcgtatccg taacgcaaac
    38041 atggttaaac atgagtcttt agaagtacct gcatcaaaaa ttaaacgtga catcgctgaa
    38101 atcttgaaac gtgaaggttt cgttcgtgat gttgaataca tcgaagatga caaacaaggc
    38161 gtgatccgtg ttttcctaaa atttggtaaa aacggcgaac gtgttatcac aaacttaaaa
    38221 cgtatctcta aaccaggttt gcgtgcttat gtcaaagctg acgaagtacc aaaagtattg
    38281 aacggtttag gtatcgcaat catttcgact tctgaaggtg ttgtcactga taaagaagct
    38341 cgtgaaaaaa atatcggcgg cgaagttatc gcttacgtat ggtaatcaag tagaatctac
    38401 aaggaggtgt ctttaagtga gccgtatcgg aaataaagtc gttgttatcc ctgaaggagt
    38461 aacagttact caagacggaa acaacgttac agttaaagga ccaaaaggtg aattgactcg
    38521 tactttttca gcagatatca aaatgaatat cgaaggaaac gaagtaactt tcactcgtcc
    38581 aaacgacagt aaagaaatga aaacaattca tggaacaact cgtgcgaact tcaacaacat
    38641 ggttgtaggt gtcagcgaag gtttccaaaa agcgttagaa ctaatcgggg ttgggtaccg
    38701 tgcacaatta caaggatcaa aacttgtatt gaacgttggt tactcacatc cagtagaaat
    38761 cacaccacca gctggagtaa ctgttgaagt accagctaac acacaagtaa ttgttaaagg
    38821 tgcgaacaaa gaagaagttg gcgaattagc tgcaaacatt cgtggaactc gtcctccaga
    38881 accttataaa ggtaaaggta ttcgttatgt tggcgaatat gtacgccgta aagaaggtaa
    38941 aactggtaaa taatcgttta gaaaaattgc atagaagcca gcagcaggaa aaaggcattt
    39001 ggctgaagtt taccttcaaa aaaatacatt tttacttgtg ccgcagcttc tatttttcga
    39061 aacgtattta ctgcttgaaa tagtagtaca ataaaaataa agaggtgaca attgtgatta
    39121 caaaaccaga taagaacaaa acacgtcaaa agagacatcg tcgtgtacgt aacaatatct
    39181 ctggtactgc tgagcgccca cgcttgaacg tttttcgttc taacaaaaac atctacgctc
    39241 aaattattga tgacgtagcg ggtgtaacgc tagcaagtgc ctctaccttg gataaagaaa
    39301 tttcaggtgg aacaaaaaca gaagcagcac aagctgtcgg taaactagtt gctgaacgtg
    39361 cagctgaaaa aggcattaaa gtagtagtct ttgaccgtgg tggatacctt taccatggcc
    39421 gtgtgcaagc tttagctgaa gctgctcgcg aaaatggact agaattttag aagaaggagg
    39481 aataccattc atggtttata tcgatccaaa acatttggaa ttagaagacc gcgttgttgc
    39541 aattaaccgt gtaacaaaag ttgttaaagg tggacgtcgt ctacgttttg ctgctttagt
    39601 tgttgtcggt gacaaaaacg gacacgtagg gtttggtaca ggtaaagctc aagaagttcc
    39661 tgaagcaatc cgtaaagcaa ttgaagatgc gaagaaaaac ttagttgaag tacctatggt
    39721 tggttcaaca atcccacacg aagtaatcgg tgcttttagt ggtggtagaa tcctaatgaa
    39781 acctgctgta gaaggttctg gggttgctgc tggtggacca gttcgtgccg tattagaatt
    39841 agctggggta gcagatatca cttctaaatc tttaggctca aatacaccaa tcaacgttgt
    39901 tcgcgcaaca gttgaaggtt tgaaacaatt gaaacgtgca gaagaagttg cagaacttcg
    39961 cggcaaatca gttgaagaat taatcggata aggaggacaa caataatggc tgaattaaaa
    40021 gttactttaa aacgcagtat tatcggacgt cctcaaaacc aacgcgatac agttaaagcg
    40081 ttaggtctta caaagattaa cagttcagtg gttaagcctt ctaatgaagc aattaaaggc
    40141 atgatcaata ctgtttctca cctagtggac gttgaagaaa tttaatttag aaaaatcata
    40201 actagaacat taaggaggtg ccaaacctat gaaacttcat gaattaaaac ctgctgaagg
    40261 ttcacgccaa gtacgtaacc gtgttggtcg tggtacttca tctggtaacg gaaaaactgc
    40321 cggacgcgga caaaaaggac aaaaagctcg ttcaggcggc ggtgtgcgtt taggatttga
    40381 agggggacaa acaccattgt tccgtcgttt accaaaacga ggattcacaa acgtgaaccg
    40441 taaagactat gcagtcatta atcttgatgt attgaaccgc tttgaagacg gagctgaagt
    40501 aactcctgtt gctttagttg aagctggaat cgtgaaaaac gaaaaagctg gaatcaaagt
    40561 attagctaat ggggaactaa acaagaaatt aactgtgaaa gcagcaaaat tctctaaagc
    40621 agctcaagaa gcaattgaag cggctggtgg atcaatcgag gtgatctaat gtttagacta
    40681 ttaaaagatg cttttaaagt aaaagacatt agatcgaaaa tcctatttac tgtatttgtg
    40741 ttgtttgtat ttcgcctagg tgcacatatt actgtaccag gcgtaaatgc tgcttcattg
    40801 caaaaccttt cttctttgcc atttttcaac atgttgaact tggtaagtgg tagtgcgatg
    40861 caaaactttt ccattttctc aatgggggtt tcaccataca ttactgcttc tatcgtcatt
    40921 cagcttcttc aaatggacat cgtgcctcga tttgttgaat ggtcgaaaca aggggaagtt
    40981 ggtcgtaaaa aactgaatca agcgactcga tatctgacat tagtattggc atttatccaa
    41041 tctgtcggta tcactgctgg attcaatcaa tacgctcaat taggtttcgt gaataatcct
    41101 aacgtagcaa cttacgtaac gatcggtttg attctaacag ccggtacgat gtttgttact
    41161 tggctaggag aacagatcac agaaaaaggt atcggaaatg gcgtatcaat gatcattttt
    41221 gcaggtatca tttctagatt gcctgaaagt atcagagaaa tttatgaaga ttactttata
    41281 aatattgatc cttcagacat atggaaatct gctatttttg tggccatttt actagtagcg
    41341 gtcttgttga tcgtgacatt cgtaacattt ttccaacaag ctgaacgcaa gatcccgatc
    41401 caatatacga aacgcgtagc tggcgcgcct acaagcagct atctgccgtt gaaagtaaat
    41461 gcagctggag ttattccagt tatctttgct agttcgttta ttgctacgcc aaatgctgtt
    41521 ttacaagcat tgagcggtag attcggttct gaaaactggt acaaagtgat gatggagatc
    41581 tttaactata atacagcacc tggtgctacg atttatacga ttctgatcgt tgccttcact
    41641 ttcttctacg cctttgttca agtaaaccct gagaaattag cggaaaactt gcaaaagcaa
    41701 ggaagttata taccaagcgt acgccctggt aaggggactg aagagtatgt ctcaagatta
    41761 ctgatgcgtt taagtacagt tggtgcagtc ttccttggtt tagtggcatt gcttccaatc
    41821 attgctcaaa tggtttggga tcttccacaa tcaatcggat taggcggtac aagcttactg
    41881 atcgttatcg gtgtagcttt agaaactgcg aaacagttag aaggcttaat gctgaaacgt
    41941 aaatatgttg gattcattga gtaagaaagc gtgttgaggt ttcccctcaa cacacgttct
    42001 cacacattta ttaggaggga aaacaatgaa cctcatttta atggggctgc caggcgcagg
    42061 taaaggaaca caagctgaaa aaatcattga tgcttatggt attccgcaca tctcaacagg
    42121 agatatgttc cgcgcagcga tgcaaaacga aacagctctt ggcctggaag caaagtctta
    42181 tatggataaa ggtgcattag ttcctgatga agtaacaaat ggaatcgtga aagagcgact
    42241 agctgaacca gatacagaga aaggcttctt attagatggc tttccacgta cgctagatca
    42301 agcaaaagct ctggacgcaa tgctaaaaga tttgaataaa aaaattgatg ctgtcatcga
    42361 tatccatgtt ggtgaagaaa tcttggtcga acgtctagcc ggccgattta tctgcagcaa
    42421 ttgtggagca acttaccata aaattttcaa ccctacaaaa gttgaagaca catgtgatcg
    42481 ttgcggtggg catgaattct atcaaagaga agatgataaa cctgagacgg ttaaaaatcg
    42541 tctggctatc aacatcaaaa atagtgaacc aatcttggct tattataaag aacaaggttt
    42601 gctaaacact attgatggtg ctcgtgaaat cgatgctgta tttgcagatg tacaaaaaat
    42661 cattgaaaaa tagcacgatt gcccgtagcc gacttgcatt atgcagttac tcttgtcaag
    42721 ttcaataaat tatgatagaa tatgtcagtc cgacttctag agaatcctct agatgatcca
    42781 ttttgagaat tttgaggtgg gaactgcagt ttccgccatt ttatcgtgtg aaaatgcgaa
    42841 acaagtacaa ggaggtactg tgcgtggcaa aagaagatat gattgaagtc gaaggtacag
    42901 tcgtcgaaac tttgccgaat gcaatgttta aagttgaact agaaaacgga caccaagttc
    42961 ttgctactgt ttcaggtaaa attcgtatgc attacatccg tatcttacct ggtgacaaag
    43021 taacggttga attgtctcca tacgatctta accgtggccg tattacttat cgctttaaat
    43081 aattgtactc cgtaaaacta caggaggtat tatcatgaaa gtaagaccat cagtaaaacc
    43141 aatgtgcgaa cattgtaaag taattcgtcg taaaggacgc gttatggtga tttgcccagc
    43201 aaatccaaaa cataaacaac gtcaaggata attggaggtg ctataaatat ggctcgtatt
    43261 gcaggagtag atattcctcg tgataaacgt gtagtagttt cacttactta tatctatggt
    43321 attggtaaca ctactgctaa aaagatctta gctgatgctg gtgtatctga agatgttcgc
    43381 gttcgtgaat taacgaatga acaaacagat gctatccgtg cagaagtaga taaattaaaa
    43441 gttgaagggg atcttcgtcg tgaagtgaac ttgaacatca aacgtttgat ggaaatcggt
    43501 tcttaccgtg gaatccgtca tcgccgtgga ttgcctgttc gtggacaaaa cacgaaaaac
    43561 aatgcacgta ctcgtaaagg cccagctcgc gctatcgctg gcaagaaaaa ataattctta
    43621 agtgaaggag gttaaaactt catggcagca aaaaaagtga atcgtaaacg ccgtgtgaag
    43681 aagaatatag aagcaggtat tgcacatatc cattctacat tcaataacac tatcatcatg
    43741 atcactgata ctcacggaaa tgcattagcg tggtcatcag ctggttcatt aggtttcaaa
    43801 ggaagcaaaa aatcaactcc ttttgccgct caaatggcag cagaaactgc tacaaaagca
    43861 gcaatggaac acggattaaa gactgttgaa gtaacagtta aaggacctgg ttctggacgt
    43921 gaagcagcaa ttcgttcatt acaagctaca ggtttagaag tgactgcaat tcgtgacgtg
    43981 actccagttc ctcataatgg tgcccgccct ccaaaacgcc gtcgtgttta atgagtgccg
    44041 ctcattttga gttttatcag taacgtcatt gaacaagaca cttttcgttt tgaaaggggt
    44101 aaagataaga atgattgagt tcgaaaaacc aagaatcgca aaaattgatg aagaaaaaga
    44161 ttatggcaag ttcatcgttg aacctcttga aagagggtat gggactactt tagggaattc
    44221 cctacgtcgt attttattat cttctttgcc aggtgctgcc atcactaata ttcaaatcga
    44281 tggtgtactg catgaattct ctaccatcaa aggtgttaga gaagacgtca ctcaaatcat
    44341 cttgaacatt aaaggcttag cgctgaaaat gtatggccaa gaagaaaaaa cccttgaaat
    44401 cgatattacc ggacctgcta cagtaactgc tggcgatatt atcgttgata gtgatgtaga
    44461 aattcttaac aaagatatgt acatctgtag tgtctctgaa ggagctacgt tccatgctcg
    44521 cttaacagtg aaacctggac gtggttatgt tcaagcagat gaaaataaaa aagaagatat
    44581 gccaattggc gttcttccag tcgattcaat ctacacacct gttcgtcgtg tgaactatca
    44641 agttgagaac acacgtgttg gacaccgtga agattttgat aaattgacga tggagatttg
    44701 gacagatggt tcaattgaac cgttggaagc aatgagtttg tctgcgaaaa tcatgactga
    44761 acatttggat atttttgtga acttaactga tgaagcaaaa aatgctgaaa tcatggtcga
    44821 aaaagaagaa acacaaaaag aaaaaatgtt ggaaatgacg atcgaagagt tagacttgtc
    44881 tgttcgctct tacaattgtt tgaaacgtgc aggaatcaat actgtacaag aacttacaaa
    44941 taaatctgag cctgaaatga tcaaagtccg taacttagga cgtaaatcat tagaagaagt
    45001 taaagcaaaa ttgcacgact taggtttagg tttacgtaaa gaagattaaa accttttacg
    45061 aaggagggaa accaacgtga gttatcgtaa actaggacgc acatctagcc aacgtaaagc
    45121 gatgttgcgt gatttaacaa ctgatttatt aatcaacgaa cgtattgtga ctactgaagc
    45181 tcgtgcgaaa gaagttcgct caactgcaga aaaaatgatc actttaggta aacgcggaga
    45241 tttgcatgca cgtcgtcaag ctgcggcttt cgtacgtaat gaagttgcca gcgtgcgtga
    45301 agaaaatgag gacatcgtca ttgaatcagc tttacaaaaa ctgttcaacg atattgcacc
    45361 tcgttacgca gaacgtcaag gcggatacac acgcattttg aagacagaac caagacgcgg
    45421 tgatggtgca ccaatggttg tcttagaact tgtctaaaat cacactaata acatcacttt
    45481 atagatgatt cttttagagt gttatgatgt tggaagatat ttttccgagt ctagctcagc
    45541 gctgctcctc aagcgaatat cgcttgggga gtaagcactc atcataaagt gtttttttat
    45601 accttcaatt acctgtcatg acagatatgt ttgaaggcat aaaaaaacat tttaagcgag
    45661 aaataattga aaattttgtc tttttttgat agtgttagta ggaactttag gatgaagaag
    45721 gagtaatcat ggagccaatt attgaattag aaaagataaa ttataaatac cagccagatg
    45781 atcttcgccc cgcattgaaa gatgtctcct ttacaattga taaaggtgag tggatcgcta
    45841 ttattggaca caatggttca ggaaaatcga cacttgccaa aacgatcaat ggattgcttc
    45901 tgccagaatc agggatcgtg aaagtgggaa atcaaatatt ggatgaagaa aatatttgga
    45961 cgatccgaca gatggttgga atgatttttc aaaatccaga caatcagttt gttggttcga
    46021 cagtagaaga tgacgttgct tttggtcttg aaaatcaagg gattcccaga gaagaaatgc
    46081 tagtgcgtgt gaaagatgca cttgagaaag ttagaatggc tgagtttgct tcacgcgagc
    46141 ctgctcgttt atcaggaggg caaaaacaac gtgttgccat tgccggcgtg gttgccttac
    46201 gacctgatat cattatttta gatgaagcaa ccagtatgct agatccagaa ggtcgtgaag
    46261 aagtgatctc tacgatcaag aagatcaaag aagaaagtca attgaccgtt atatctatca
    46321 ctcatgatat tgatgaagca gctaatgcca atcggatact agtgatgaga caaggcgaac
    46381 ttgtccgtga aggtacacca aaagaaattt tttctgctgg tcctgaactg atcgacttag
    46441 gtctagatct accatttcct gaaaaactga agagcgcact gaaagaacgt ggggttgatg
    46501 tgcccagtga atatatgaca gaagaaagga tggtggattg gttatggaca tccgttttga
    46561 acaagtagac ttcacttatc agccgaatac accatttgaa caaagagcct tatttgacat
    46621 caatatgacg atcaaagaaa acagttatac tgcattagta gggcataccg gaagcgggaa
    46681 atcaacctta cttcagcatt tgaatgcgct tgttaagcca acaagcggta cagtccatat
    46741 tggagaacga gatattcagc cggatacgga taataagaat ttaaagccca tccgaaaaaa
    46801 agttggcatc gtctttcagt ttccagaagc acagctattc gaagaaacgg tagcaaaaga
    46861 cattgctttc ggtcctaaaa actttggtgt cagcgaagaa gaagcactag tcctagcaaa
    46921 agaaacatta gaacaagttg ggctggatga aagctatttg gaacgttcgc catttgaact
    46981 ttcaggaggg caaatgcgta gagtagcaat cgctggtgtg cttgccatga ggccggaagt
    47041 acttgtattg gatgaaccaa cggcaggact tgatccccag ggcagaaaag aaatgatgga
    47101 gatgttttgg cgtcttcata aggaacagca gatcactatt gtattagtta cgcatttaat
    47161 ggatgatgta gccaattttg cagattatgt ctatgtgttg gaaaagggac gaatcgttaa
    47221 tagcggggag ccgcaagagg tctttcaaaa cattgaatgg ctaaaagaaa aacaactagg
    47281 cgtacctact gccaccgagt ttgcagaaga attaatggct aaagggatga actttgctac
    47341 cttgcctttg acagctgaag aattagcaga cgctatcgtc cattatgctg gaggaaatgc
    47401 ccatgatgaa taaattgata tttggtcgtt atatgcctgg agattccttg atccatcgat
    47461 tggatccccg agctaaatta ttagccagtt tttattttat cggaatcatt tttttagcaa
    47521 ataattggga aacgtacctt ttgttggcat ttttcacatt actttctgtc ttcttgtcaa
    47581 aagtagattt gaaatttttt atccgaggca ttcgcccatt gatctggcta atcctcttca
    47641 ctgtagcttt gcaggtcttg tttacaagtg gcggagaaac ttattggagt tggggtgttt
    47701 tccatatcac tgaatttggt gtgatgaacg gcctctttat tttctgtcga ttcgtgttga
    47761 tcatttttat gtcgactcta ctcacgctta ctacaccgcc tttggaatta tcggatgcga
    47821 ttgagtactt gcttcgtcca ttgaaagccg tacggttccc agtccacgaa atttcattga
    47881 tgctgtcgat tgctcttcgt tttgtgccta cattgatgga cgaaacagaa aagatcatga
    47941 acgctcagcg cgctcgcggt gttgattttg gtgaaggaaa cctagttcag aaaatgaaag
    48001 cagtcattcc tttgctgatt cctttatttg tcagcagctt caaccgtgcg gaagatcttg
    48061 cgactgctat ggaagcaaga ggctacaaag gtggggaagg aagaacgaaa tatcgtgtat
    48121 tgcattggca taatagagac acgatggtta tcattgcttt tgtattatta actgtgctac
    48181 tgatcttttt aagaggataa acgttcagtg ggccgctatc tattcttttc ttgctgatct
    48241 gtttgcaaga aaggatgcaa gcagtgtatg acaagaaata ttagggtctg tggcagacta
    48301 aggtcacagg cctctttctt ttatctgaaa agaaagaacg aaaaaatttt atagaaaaat
    48361 cattattcca gataaccttt taggaacaaa ggagagaaaa acatggtacg ttacaaagca
    48421 atcattgcgt acgacggaac aaattttaat ggattccaaa aacagccaaa tgggcgtact
    48481 gttcaagaag aagtagaaaa aacattacaa aaaatggcaa atggaaaaga aatcacggtc
    48541 tttggttcgg gaaggacaga tgccggagta catgcgatcg ggcaagtaat ccattttgat
    48601 tatccagagg agcgtccgtt agaacgaatg cgtttcgcgc tagataccca gtcaccagag
    48661 gatattgctg taagacaagt agaaattgtt tcagaagact tccacgcaag atatttggtc
    48721 aaagaaaaga cgtatcaatt tcgagtggat atagggaaac cacgcagtcc ttttcgacgc
    48781 cattatgcaa gttattttcc ttacccactt gatttggaaa aaattcagcg ggcgcttcct
    48841 gatttattag ggactcacga tttcacctct ttttgtgcat caggaagctc gatagaggat
    48901 aaaatacgta cgatctacga ggctaagatg gaggtaaatg aaacaaaaga cgaattgttg
    48961 tttacattta ggggaaatgg cttcttgtat aagatgatac ggatcttagt aggaacgctt
    49021 ttgaaaattg gcaatggccg attgcctgaa gatagtatcc cagatattat tgcgaaaaaa
    49081 gatcgtaatg cagcaggacc tacagcacac cctgaaggac tttatttgta tgaagtgaaa
    49141 tatgaataag aggttatgat agaagtggtc agcttcaaga aataagaagg tatttacgaa
    49201 aattacttct caaaattttg caaaatttcg gcttattttc gaagaagcta cttctggaac
    49261 accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaaa gagcaatttc
    49321 aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg
    49381 aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat
    49441 tgcccttggt gcggacagtc tgaacaggtt ccatcgtttc tttattttct acgtcttctt
    49501 ctgtgtgtgt ttcgtggttg ttcttctatt gctggatcaa tcgtgccata aaaggggcga
    49561 ttgcggtcaa ggtaccagac ataaagaata aacaatgcaa tacataggac aaaaaggatt
    49621 gcacctatcg taaatccttc aggcaagtaa ctgaaagtga cagtatgttt acctgctgga
    49681 acttttatca tcaagaatgc gtctttgaaa ttgctgacct tgactggttt cccatcaatt
    49741 ttagctttcc atcctttgtc ataaggaata gttgtcacca acatggaatc ttcagcggta
    49801 tttacagtcc cagaagcttt tctgccagtg gcttccattt cgacaccttt ttctttgatc
    49861 tgattcattg cggcttgata ggctacaaca tctagaccaa cgactttagg ctccatgaaa
    49921 cttatttctt tcgtcccata aaaacttgca gtaaatgtca ctgttgtctc ttgaggataa
    49981 tagccaagat catagtattg accagaaatg ttgatttgcg tttttcgcga tgtaccattg
    50041 acagtgatcg tcgcactaga actttctaat tgcgcaaaat tggttgggaa caaacttaag
    50101 taagcttgtg tatttgcagg cacatggact tcccaagtga gctcttttgg ctggttatct
    50161 gctgtttctg agtaagtgac gccagtcgtt gtctttgtga tcgtcgtatt caactgattc
    50221 ttcaaagtag gtgtatagaa tgtaaaatac tgttcattta gaccagacaa tgtattgatc
    50281 aagtttgtct gactggtcaa attatctgtc actggctgct caacatcatt aacggacaac
    50341 ggtgcagtaa atgctagtgg caatgcattg acattctgat acagtgtata gtcgccgctt
    50401 tgaccagctt ggcggaagcc atatttactg aacgtgcttt cgtttttaga aatgttatat
    50461 ttgattcctg taaacccatc catcagcaaa gtattattgt tataacggat attcaaattt
    50521 gtccctcttg aacggaaacc taatttatcc aggtaagagg aagaattacg atttcttata
    50581 gaagaaaaca aactgatacc actgtaacca tagttgatcg aatcgtttgc agatactgga
    50641 tccagatttt ccaagcggta aaacgagtcg gaatcttttt ttgtttcatt tacaagagct
    50701 ttcaagtcag gataaggatc agtatataaa ctgcgggaag cataattcca atcatcaaga
    50761 atgccatgga tcatactgtt cgtattgacc agtgcttcag cagacatgat caataataac
    50821 aatatactca gatgattgaa tgaaagtttt ttatgctgat aaaatcccac agcaaaagca
    50881 taaatcccta agaaaaccac tgtcaataca aaggaagtca tcgtcacata ggtgtagctt
    50941 ccttcaggtt tgatgcccca tgccaccgca aagagagcag ctacgaccaa aatgattcct
    51001 aacaacagcc caccatcgtc ttgttctatc ttttcccatc cttttcctgc aagcaggagc
    51061 actaagaaag agaacagata ggcataacgg aataaaaaca tattcggtgc atgcatacca
    51121 tgccagaaca aattcaatgg aacaagataa aaacttgcga taagtatggc gaaaagactg
    51181 ccgtacaaaa atttattttt caacggtact ttccgactgg caaaataaaa cagacagagg
    51241 actaatggta gcagacctgc gtaaacgaac ggaatcgatc cgtattttgt cgtgtcataa
    51301 acaccaatca tatttttcat gattagatcc agataagaag tcgcttctgt tttcaacgta
    51361 gtgatctgac tcagtgcctc gccatttgac cgaagatcca gcactgctgg caatacaatg
    51421 atcattgaag cgccgcctgc cagtaaagaa gtgataccat aaggaacaat cgttttttta
    51481 tattctgacc agtttgtcaa tagacgggcg ataaaatata agaaagaaaa tacgccaatc
    51541 ataaaaccca tataaaaact ggataaaaac aagagtaaat aactgataaa taaaagtttc
    51601 ggccgtttaa gatccataac acgatgaatc cccaaaatca ctaatggaag ataaacaaat
    51661 gcatccagcc acatgatgat ctccgaatgt gcagtgataa atgagatcaa tgcgtaactg
    51721 acgctaagtg caacatgccc ccagcgagga atcttgaacg tacgtgcaag aaaccaaaaa
    51781 gccaatccgg cacagccgac cttaagcaaa gtcagaacat acagtgcatc tggcatcatc
    51841 tggtttggaa aaaagaacac cagtggcgtg aagatcccgc ctagatagta agaaattaaa
    51901 gataaataat tcaatcctag agaagcattc catgtataaa aaatactttg ctttccaaga
    51961 agaacatttc ggaagctggc atgaaaatta gaaaactgtg aaaaggcatc acttgccaaa
    52021 atacttctgc tgcttccagg atagattccg atacttagat aaacaagtgc taaaatcaaa
    52081 aacggaatca ggaaactagc tgctgcgtag cgtccgtttt ctctaaagaa tgactttaaa
    52141 ttttttttca tatttatttt ccaccaaatt tctatgaagt tgtattcagt ttatcatatc
    52201 cttgtttttc tctgcttaaa aagagtctag ttttcacaaa aaaataaaat ttagaataaa
    52261 taacttcata atttgttcgt ataacaaaaa taaacgcaaa aaaagggtag gaatcaaaag
    52321 tcccaccctt aaaaattttt attaaataaa aaaccgttca ctatctgaac ggtccaagta
    52381 tcattcaatt aaacgcgttc aacttttcct gatttcaaag cacgagttga cacccaaact
    52441 tttttaggtt ttccgtcgac cataacgcga actttttgta agttaggttt aacagtacgt
    52501 tttgttgagt tcatagcgtg tgaacggttg tttccacttt ttgtcttgcg acctgtaaag
    52561 taacaaactt ttgccatggt aatttcctcc tttgccttga tttggagcaa tagaataatt
    52621 cagctcatac cagactaaca tatcatatag aatgggaatt tgcaagtaca ttttcataga
    52681 tacaattgac aattgagaag aataattgta aattaaacaa ggttatacct gaaattttgc
    52741 aaggtttttg agtactttgg gtaactgcct tttatttcaa gttgcgctat gataaaatat
    52801 gatagaagaa aaatgggagt tttttacccg ttaaggaggc tcattcatgg ctgtaaaaat
    52861 tcaaacgtca gctggaacca ttgaaattac caatgatgta attgcaacag ttgttggtgg
    52921 cgccgcgaca gatgtgttcg gtgttgtagg aatggccagc aaaaatcaaa ttaaagataa
    52981 tattactgag attttgcgta aagaaaatta ctcaagagga gtagttgtcc gccaagaaga
    53041 taatggtatt gctgtagatg tttatacgat tatcagctat ggtacgaaaa tctctgaagt
    53101 ttcccgcaac gtccaagaaa aagtgaaata caatctagag gcattattag gcgtaactgc
    53161 caattcagtt aatgtctttg tacaaggtgt tcgtgtactg ccagactagg caggagactg
    53221 gctggtaact cagcagtcga tctgaaggag gattatctag gtgagtgtaa cagaaataag
    53281 tgcaagccag ttccaggaaa tggtccaggc aggtgcgaat cgtctgcaag taaatgcaga
    53341 atacgtcaat tcattaaatg tattccctgt gccagatggc gatacgggaa caaatatgaa
    53401 tctatctatg actagtggag ctaaggcagt agtagattca acctcagaaa aagtaggaga
    53461 attagcagca atgctttcaa aaggattgct gatgggtgcg cgcggaaatt ccggcgttat
    53521 tttgtctcaa ttgtttcgtg gattttctaa gcaaattcca gatgttgtct cattaaatgc
    53581 ttctgatctt gctgcagcct tcactcatgg agttgaaact gcgtataaag cagtaatgaa
    53641 accagtagaa ggaacgattt tgacagtagc tagagaagca gcgaaagctg gagaaaagaa
    53701 agcaaaaagt actgatgatg tcgttgaagt aatgacagct gttgtaaaag gagcaaagcg
    53761 tgctttagct aaaactccag acttgcttcc agtcctaaaa gaagtcggtg tcgtagacag
    53821 tggagggcaa ggactgctgt ttgtttatga gggcttttta agtgcattga atggagaatt
    53881 ccaagcggat gaaacatatg aaccttctcc agcagaaatg gatgatatgg tcaatgcaga
    53941 acatcatcgc agtgtccaag gtcaaatggc gacagaagat atccattttg gttactgtac
    54001 agaaatcatg gtaaaaatcg gagaaggacc tacagtcgat agtacattcg attacgaaga
    54061 attccgtaac tatttagatg gtataggaga ttctttactt gtggtcaatg atgacgagat
    54121 catcaaagtc cacgtgcata cagaaaaccc aggagaagtc ttgaattatg gacaaaaatt
    54181 cggttcatta gtcaaaatca aagtcgacaa catgcgcttg cagcatgaga cgattttgga
    54241 acacgatgaa gaagtgaacg agtttgcgaa aaaagaaata ccacgtatcc cgtatggtat
    54301 cgtagcgatc gctgctggta aaggcgtcca agagctgttt gaaagtttag gcgctaatta
    54361 tgttatcagt ggtggacaaa caatgaatcc aagtacggaa gacattgtta aagcgattca
    54421 agaagtaaac gcagataaag tgattgtctt gccaaacaat aagaacatct tcatggcagc
    54481 tgatcaagca gcagaagtag cagatatccc agtagcagtt gttccttcaa gaacgatttc
    54541 ccaaggattg acagctatgc tttcattcaa tgatcaagct tctttagaag aaaacaaaga
    54601 aacgatgaca gaaatgttgt ctagcgttgt gagcggtcaa gtcactcacg cgatcagaga
    54661 tacctcaatt gacggtgtag agatcaaaga aggagacttt ttgggcatga tcgatggaaa
    54721 aatcctgatc tctaacccag atatcacagc aacagcgtta agtacactag aacaaatgat
    54781 caatgaggac acagagatcg taacgatctt ggcaggtgat gaaggaacag ttgaacaagc
    54841 aaatgaattt gctgagaaac taacagaaaa acatcctgat ttggaaatcg agatccacca
    54901 aggagatcag cctgtctatc cttatctatt ttctgctgaa taatttctgt gtcagaatat
    54961 ctttctaaca cagtattcat tcataaaagg ccttcggtta gtgatcaggt atgatcgctg
    55021 accaagtcct ttttttgtta aaggagggag gaaaaattta tgcgttcttt agatgatcca
    55081 atcactatgc tggcaggagt cggagaaaaa agagcagcta gtcttgccag cttgggcatc
    55141 caaacgattg aagacttact gacttattat ccttttcgct acgaagatat tcaggaaaga
    55201 aatctaaacg agatccagga tcaagaaaag gttactttga aaggaatcgt agtctcgcct
    55261 cctgtgatga atcgatttgg ttataaaaaa agccgattgc aatttcgtat gatgcaagaa
    55321 catgatgtgt tcaacgtttc tttttttaat caaccttatc taaaagaaaa agtcatttta
    55381 tcggaggaaa tcgccgtata cggtaaatgg gatgcaaagc gtaaagcctt gaacgggatg
    55441 aagattttag gttctcaaac aatggatgac ttttcgccga tatatcacgt caacaaatca
    55501 attcgtcaaa cgacattagt tgatctaatc aaaaaagggt ttcaagaata cggcacattg
    55561 attgaagaaa atctgccaaa caaccttgtg gagaaatatc gactgcttga ccgagcttct
    55621 gctgtaagaa gtatgcattt tccaaaagac cacgaagaaa accatcaagc caaacgacgt
    55681 gtcgtttttg aagagttctt tttatttcaa atgcgtcttc aaggattgaa aaaagcagaa
    55741 aaagcagaaa cgaatggttt ggagatcctt tatgatgtcc aaaaattgaa acaatttact
    55801 caaaagctac cttttgaact gacaaatgca caaaaacgtg taacgaatga aatctgtcgg
    55861 gatcttcgca gtccgcagca tatgcagcga cttttgcagg gagatgtagg tagcggaaaa
    55921 acagtggttg ccgcgattgc gttatacgct accgttacag caggtttcca aggtgcgttg
    55981 atggtgccta cagaaatctt ggcacaacaa catatggaaa gcttgcaaca gctgtttgat
    56041 cccaatgaag taaccatcgc attgattacg gggtcaacga aagcaaaaga acgacgtgag
    56101 ctccttgaaa aactagagca aggggaaatt aacgtcgtga tcggcacaca tgcattgatc
    56161 caagatggag tcgaatttca gaatttaggt ctagttatta cggatgagca gcaccgtttt
    56221 ggcgtgaacc agcggaaagt acttcgtgaa aaaggctggc gaccagatgt cttatttatg
    56281 acagcgactc cgattcctag aacattagct attacagcat atggtgagat ggatgtatct
    56341 atcattgatg aacttccagc tggacgtatc ccgatcgaaa cacgctgggt aagaacacct
    56401 caattggaca gtgtactgga ttggacgaaa aaagaactgg caagaggaca tcaaatgtat
    56461 gtcatctgtc cgctgattga agaatcagaa gcattagatg taaaaaatgc agtggaaatc
    56521 tatgaaaaac ttcgtgaact atttgcacca aaatatgaag ttggcttgtt acatggtaaa
    56581 atgaagaatc aggaaaaaga agtaatcatg gaagccttca aagacaacca gatgcagata
    56641 ttagtttcga ctactgtaat tgaagtaggg gtaaacgtgc cgaacgcgac cgtcatgctg
    56701 atcatggatg cggatcgatt tggattggca cagctgcacc aacttcgtgg acgtgttgga
    56761 cgcggaagtg atgcttccta ctgtatctta gttgctaatc ctaaaaatga actaggtgtg
    56821 gaacggatga aaatcatgac agagaccacc aatggttttg tactaagcga aaaagatctg
    56881 gaattacgtg gtcctggtga agtatttggc ttccgtcaat ctggtctgcc tcaatttgcg
    56941 attgctgact tggtgacgga tggaaatgtt ttggaggtag ccagagaaga ggctgcagcc
    57001 ctttggcaaa taaaggattg gcagatgctt cctgagtatc agcctttagc gcaaagcctc
    57061 caaatagaaa atcaggataa tcgttatttc gattagcaga ctgtgctata ctttatactg
    57121 atgacggaaa aaggagtgga aacaatgaaa attgccgtag atgcaatggg cggagatcac
    57181 gcacctcaag caattgtaga aggcgtggct ttagcgcagc aagattttcc agaagtagaa
    57241 ttcttacttt acggaaaaga agcagaaatc aagaaatact taacaaatga aaaaaacatc
    57301 acgattatcc atacagacga aaaaatcaat agtgatgatg agccagtaaa agcaattcga
    57361 cgaaaaaaga ctgcatcaat ggtccttgct gcacaagcag tgaaaaacgg tgaagcagat
    57421 gctgtatttt cagcagggaa tacaggcgcg ctactagcag cagggttgtt catcgtagga
    57481 cgtatcaaaa atatcgaacg ccctggcttg atgtctacct tgccagtagt agggaaagaa
    57541 ggtgccggtt tcgatatgct ggatcttggc gctaacgcag aaaacaaacc agaacatttg
    57601 ttgcaatacg gtattttagg ttctttttat gcacgtaaag tccgaggtat cggacgtcct
    57661 cgtgtcgcac tcttaaacaa cggaacagaa gcaacaaaag gtagtgaagt gacgaaaaaa
    57721 gcctatgaac tccttcagaa tgaaacaagc ttgaatttta ttggaaacgt agaagctcgt
    57781 gagctactca atggtgtggc tgatgtagtg gtcacagatg gatttacagg caacgctgtt
    57841 ttgaaatcta ttgaaggaac agcaatgaat atgatgaatc tattaaaatc tgctattcta
    57901 aatgaaggga tcaaaggaaa aatgggcgct atgcttttaa aagacggctt acgttctttg
    57961 aaagcagaaa tggattattc caaacatggc ggtgccgttt tgtttggctt gaaagcacca
    58021 gtcattaaaa ctcatggagc aacaggcccc gatgctgttc gctatacgat ccgccaaatc
    58081 cacacaatgc ttgagacaga tgtagttgga caattagtcg aacaatttga aaaaagcgaa
    58141 gaatagtatt tggatatctg aagcgatccg ttccattgaa aaaaaggtgg acattcctga
    58201 aaaatgccag taaaattagc catggtgtag gtaattgaca agtgtatgat tcttggaggt
    58261 gaaaaagttg actcgcgaag aagtatttaa caaagtagcg aaaattattt caaatcattt
    58321 tgagatagat accgataaag tgacggatga attgaacatc aaagatgatt taaaagctga
    58381 ttcaattagt attatggaat tcgttttaga gcttgaagat gaattcggta cggaaatttc
    58441 agatgaagac gctgaacaaa ttgaaacagt cggtggcgca gtagattata tcagcagcca
    58501 tcttaaataa tttcaaataa aagcaaagaa ggataaaaag ctgcatgcag ctttttatcc
    58561 ttcttttttt aatctttttg acacgtaaaa gtcatttttc tgataattct ggcgataaac
    58621 agaaattatg ttgcaaagta aagcggtttt ctatataatt attaataatt cattacacaa
    58681 agataagaat aacctttgtt ataaaacgat ttattctgtg taatgtcctt ttttattaag
    58741 aaaaacttcc atttaggaag cagagagggg tattcgattg tccgataacc aattattaga
    58801 agttcagaac ctacatacag gattccgact caaagatgac tattacgatg cagtagatga
    58861 cgtttcattt actttagaca aaaacgagat tttagcaatc gttggggaat ctgggtgcgg
    58921 gaaaagtacc ttggctacta cgattatggg attacttgat cctaataaca caaaaattac
    58981 aggtgagatt ttatataacg atctgaattt aatcgacctg aacgaaacgc tctataataa
    59041 aatccgcgga aatgatatcg gaatgatttt ccaagatccg ttgtctgcat taaatcctct
    59101 catgagaatc gaagaccaga tcaaagaagg actttcctat cacacaaaga tgaatgcaga
    59161 acagcgtcaa gctcgtgcac tagaattgct agaacaagta gggattccta atcctgcgag
    59221 agttggacgc caatatcccc atgaactatc aggcggtatg agacaacgtg tcatcatcgc
    59281 aatcgcaatc gcctgcaaac ctccaatcgt cattgcagat gaaccgacga cagccctaga
    59341 tgtaaccatc caagcacaga ttcttgattt actgaaagac cttcaggaag aaacaaaaac
    59401 agggatcatc ttgatcactc atgacttagg agtcgtagca gaaatggctg atcgagtggc
    59461 agtgatgtat ggcggacagt tcgttgaagt agcaggtgta aaagaactgt ttgatcatcc
    59521 taagcatcct tatacacgct ctttactgaa ctctatacca caagaaggaa atcatgatgc
    59581 agaactccat gtgatcgaag gtgtcgttcc ttctctgaaa aacatgcctc gtaaaggttg
    59641 tcggtttgca ccacgtattc catggatacc agcaagcgat catgaagaag agcctatcct
    59701 tcatgaagtc gcaccgaacc acttggtacg ttgctcctgc tataagcatt tccactttag
    59761 agacgaaaaa ggagaggtgt agatgacaga attaatcaca atcaaagacc tgaaagttca
    59821 ttaccctatt cgcagtggat tcttcaatcg agtaacggat catgtatatg ctgtagatgg
    59881 tgtggatttt attatcgaaa aaggaaagac atatggttta gtgggagagt ctggctctgg
    59941 aaaatctact acgggaaaag ccgttgtggg attagaaaag gtaacagccg gacaaatcat
    60001 gtatgaagga aaagatgtaa cgaagcgaag caaccgcaaa aaaatgggtt ataacaagga
    60061 tgtgcagatg atcttccaag attcaatgtc cagtttgaat cctaaaaagc gagtgttgga
    60121 tatcattgct gaacccatcc gaaattttga acggttaagc gatcaagaag aaaagaaaaa
    60181 ggtaaaagga ctgctagata tcgttgggat gccggaagat gcgctatata aatatcctca
    60241 tgaattttca ggcggacaac gtcaacgtct aggagtagcc cgtgctgttg ctaccaatcc
    60301 aaaactgatc gtagccgatg aacctgtatc tgctctggat ctatctgttc aagcgcaagt
    60361 attgaatttc atgaaacgaa tccagcaaga atacggcctc agctatttat tcatttcaca
    60421 tgatttaggt gtagtaaaac acatgtgcga caatatcgca attatgtaca aaggtcgatt
    60481 tgtagaaatc ggtacaagag aagacattta taatgacccg cgacatatct atacgaaacg
    60541 tctgctttca gctattccgc aaatcgatgt agaaaatagg gaaagccaca aagaaaatcg
    60601 acgaagagta gaacaagagt atatccaaaa ccaaaaagaa tactatgatg cgactggtcg
    60661 agtctacgat ttgcgtacga tcacaactac acacaaagtg gcgttgaaag atggaggtgc
    60721 aagctaatgt ggaaaacgat tctgcgacgt gttttattga tgatcccgca agttattatt
    60781 ttaagtgttc tgatcttctt attagcccaa gcgatgccgg gggatccatt taccggtttg
    60841 atcaatccaa atcaggatcc ggcagtgatc gaacagatgc gacaagcagc tggcttgaat
    60901 gacccatggt atgtccagta cttccgttgg atcggaaatg ctttgcaagg cgattttggt
    60961 caaagtttct tatacaaacg ctctgttgct gacttgatcg gcgaacggat catcaacact
    61021 ttatatctat cgatcttgac agttgtcctt acttatttga ttgctgtgcc tctggggatg
    61081 cttgctggcc ggtatcaaaa ttcaatcttg gataagattg tcgtaatcta caacttcttt
    61141 agttttgctg ttcctttatt tatcttcggc ttgatcatgc tatttgtttt tggttatcgt
    61201 ttaggctggt tcccaacaag tggatcacaa acactaggtt tgcaaggtgg atttatcact
    61261 caatggatag accgtttgaa atatattctg ttaccatcaa ttacccaagc atttttggcg
    61321 actgccgtca caatccaata tctgcgaagt gaagtaatcg attcgaaatc gctcgatttt
    61381 gtcagaacag cccgttccaa aggggtaccg acgaataaag tattcagtcg acatattttc
    61441 cgaaatgcag cgttgccgat ggcttctcaa atggggtatg aaatcacttc gttgattgct
    61501 ggttctgtag ttatcgaaaa gatttttggt tatccgggag tcggaaaact atttattgat
    61561 tccatcggcc aacgggacta taccgtgatc acatccttag tattgatttt aggaatcgcg
    61621 acgctagtgg ggacattact ttcagatatc atcatgagta tcgttgaccc gcgtattcgg
    61681 attcaataaa accaatcaga aaggagaatg gatcgatgag tgaaaaaaac aatacagcag
    61741 ttcaagaaag tattcctcct atggggatac gaatgattgc tcgagaattt aaaaaagata
    61801 aaattgctat tttttcatta ggtttgctag tggttttatt attagtgatc tttatcggtg
    61861 cgatgctgac cgatcaagac aaagtgatgt atgttagtat cttcgacaaa tacgctgcac
    61921 caggaacagt ttcgacacaa ggacaaaagt tcattctggg agcagatgaa ggcggacgtg
    61981 atgtattagg acaattgatc ataggtgcta gaaactctgt tacgatcgga tttgcgatca
    62041 cgatccttac ttcgatcatc ggggttggat tagggatcat ctctggctac tacggaggga
    62101 tcattgacaa catcttgatg cggatcgttg attttatcat gattctaccg atcatgctga
    62161 tcattatcgt cttcgtatcg attatttcca gatacaatgt ctggtccttt atattcatca
    62221 tgagtgcatt ttattgggta gctaaagccc gattattccg aagcaagaca ttatctgaag
    62281 caagaagaga ttatgtcagt gcctcaaaaa caatgggaac caatgatttt aaaatcatgt
    62341 tccgcgaaat catgccgaat ctaagttcat taatcattac taatttgacg atgaactttg
    62401 cggcaaacat cggtatcgag acaactttga ctttcttagg tttcggcttg ccggcaaatg
    62461 tacctagttt aggtacattg atcggttatg caagtaacgg agaagtctta gtgaatcggc
    62521 aatggatctg gttacccgca tcaatcctta ttttagtttt gatgttgagt atcaattatg
    62581 ttggacaagc attcaagcgt tctgcagatg cgcgccaacg gttaggctag tatagaagcg
    62641 aagggggaaa taggaatgaa gaaaacgatt tttggatttg cagcgctgat gtctgctgtc
    62701 gttcttgcag catgcggggg cggaggtaca gatacctctg gaaacaactc tggaggcaac
    62761 ggcaataaca aagaggaatt aacttttcca attgcaacaa aaaataatga tgatgcaatc
    62821 gaagacggcg aattagaagc tgcggtcgca acggacagtc agtttaaagg attgttccag
    62881 tgggagtttt accaagattc atatgatgca gcttttatgg ctccttctca tgaaccgcta
    62941 tttgcaaacg atgacgattt cagaatcaca gacgacggag ctgcaacgct tgatctagat
    63001 gaagatacga aaaaagcgac aatcaaagta aaagacaacg tcaaatggtc agatggtgaa
    63061 gatgtaacgg cggacgatgt gattttccct tacgaaatca ttgggaataa agattacact
    63121 ggtatccgtt atgacgacac tttccgtaac atcgtaggta tggaagaata tcatgatgga
    63181 agtgccgaca caatctccgg aatcaaaaaa atagacgaca aaacagttga aatcgaatat
    63241 aaagaagtaa acccaagcat gcttcaatcg ggtggtggta tctggtcata tgcagcacca
    63301 aaacatacgt tagaaggtgt agcaatcaaa gatatggaat caagcgatca agtacgtaaa
    63361 aatccagtga cttttggccc ttattacatg agtaatatcg ttgcaggaga atcagtagaa
    63421 tacttgccaa acgagcacta ctggaatggt acgcctaaat tgaaaaagat cacgatgaaa
    63481 tctgtaccga tggcaagtgc aactgaagca ttgaactcta aattatatga tatgattttc
    63541 caaatgccaa cagatactta cgatacctat aaagaaattg ctggctatac aaacttaggc
    63601 cgtgaacaaa cttcttatac ttatctagga ttcaagttag gtaaatggaa tgcagataca
    63661 aagagtgtgg aatacaatcc agattcaaaa atggctgata aatcattacg tcaagcaatg
    63721 ggctatgcat tagataataa tgcagtaggt gaacgtttct atgcaggttt gagaagcaat
    63781 gcgacttcat tgattcctcc agtattccag tcataccatg atgatgagat gaaaggattc
    63841 acacaagatc tagacaaagc gaaaaaacta ttagatgatg ctggctacaa agatgtggat
    63901 gacgatggtt taagagaaga caaagatgga aataaactaa caatcaactt tgcttcgatg
    63961 tccggtgggg aaacagcgca accattggca gattactata tccaacaatg gaaagaaatt
    64021 ggtttggatg tccaatacac aacaggtcgt ctgatcgaat tcaattcttt ctatgataaa
    64081 ttagaaaacg atgatcctga aattgatgtt taccaagctg cttggaatac aggaactgat
    64141 ccaaatccaa gtggactatg gggaccaaat gctgcgttca actacactcg ttatgaatca
    64201 gaagaaaata caaaattgat caacgatatc agttcaagca aagcatttga tgaagagtac
    64261 caaaaagaag catatcaaaa atggcaacaa tatgcatttg aagaagcatt tgcgattcca
    64321 acgttgttcc gtaatgaagt attgccagtc aacgaccgtg tcaaagactg ggattggtct
    64381 tatgatgtac caaacccatg ggcaaatgtt tctgttacag cagattcacg ttcataatca
    64441 ctaaaaatga ccgaaaaagg gcgatgtcct ttttcggttt tttgatattt ttctgtttat
    64501 ttctctaata ttcaggtcaa ggcgctgatt tcatgacttc tccaataaac ggtgttcaaa
    64561 gtccagtttc tcggtattaa gtcaaattta tgcaaaaata tggaaagcca ttttcacaaa
    64621 attttttctt catactcgaa actaaacgaa tttttcacaa cttctcgtca atcactagga
    64681 ttattaaagc tttacttacc ttttgaccga accatttcaa atttgacaaa aaaccagtat
    64741 aataaggaag tatcgaaaaa atatccataa aaggaatgtg gaaaatggac aaccagctga
    64801 caaaagaact aaaagaaaac tataacatcg tttttcacga tctaaatttg cttgaacaag
    64861 cttttaccca ttcatcctat gtgaatgagc atcgcaatct gcaattatcg gataatgagc
    64921 gacttgaatt tttaggagat gccgttttag aactgatggt ttctcagtat ttattccgtt
    64981 tatatcctca tttgccagaa ggaaaattga caaaaacacg agctgcgatc gttcgcgaag
    65041 acagcttgtc gaaatttgcc aaagaatgtc actttgatca atatgttttg ttgggcaagg
    65101 gagaagagaa ttcaggagga cgtacgcgac cagcattgct ttgcgaccta ttcgaagctt
    65161 ttttaggcgc gttatatctt gaccagggat tcgacacgac ccatgcattc atcgaaaaag
    65221 tgatttttcc taaagtcaaa gcaggtgctt tttcacatga gatggatcat aaaacaaaac
    65281 tacaagaagt cttgcaaaaa tcaggtgatg tatcaatcga ataccgattg atcaatgaag
    65341 aaggtccagc tcatgaacga gtattttgga tcgaagttta tgtagatgat caattgattg
    65401 gcactggaca agggaaatcg aaaaaattag cagaacaagc tgcggcggaa aatgcattgg
    65461 cagcactgta aaagtaagtg agggaaagct ttgtatttga aacgaattga aatagccggt
    65521 tttaaatcat ttgcagacaa gacagtcatt gattttgaaa atagcgtcac agcagtggtt
    65581 ggaccaaatg gcagcggaaa aagcaatatt acagaggcta tccgctgggt attaggagaa
    65641 caatctgcta aaagtctgcg aggcggaaaa atgccggata tcatctttgc cggttccgat
    65701 acccgtaagc agctgaatat tgcagaagtc acagtgattt tagacaatag tgatcattat
    65761 ctgccattgg agtacaatga gatcagtgta acccgccgtt atcgacggac tggggaaagc
    65821 gagtttttca tcaataaaca aagctgcaga ttaaaagata tccaagaatt gttcttagat
    65881 tctggattag gaaaagaatc gttctcaatc atttctcaag gaaaagtaga agcgattttc
    65941 agtagtaaac cggaagaccg tcgtgggatc tttgaagaag cagctggtgt cttgaaatac
    66001 aagcagcgga aaaagaaagc agaacagaag ctttttgaaa cagaagacaa cctcagccgc
    66061 gtccaagaca tcatccacga attggaagaa cagttaactc ctttggccgc ccagagtgag
    66121 gcagcaaagg agtttttacg gctaaaagaa acactgactc aaaccgatgt ctctttgatg
    66181 gttgctgaaa tcaagacagc aaaaaaagat tgggacaaca aacaagcaca gcttgcgaaa
    66241 ttcaatctag aattaggcaa actatctgag tcgatccaag aacaagaatc cattttggcg
    66301 aagcagcgta aagaaaatgc gcaagccgat cgtctgatcg aaaaaaatca gcaagtatta
    66361 ttagatcttt cggaaaaact gaagcagaca gaaggacaaa aagatgtttt gcaggagcgg
    66421 acaaagcata cacaaaaaag ctcgcaagaa tatcaaactt cattagcaga agcacaaaaa
    66481 aaggttaaac attttgaaaa gcttcaagaa agcttgatga aagcagcagc agaaaaagaa
    66541 acagagatcc aaaaagctga agcaaatctc ataaaaacac agcaagaatt agaaaaatac
    66601 caaaaatcaa cgaaagaatt attggcagaa ttaagagatc aatatgttga tttgatgcaa
    66661 gaacaagcag ctgtaggaaa cgaactgaag tatctagaac gtcaatatat acaagaaaca
    66721 gcaaaaagca agcagacgtt agccaagcaa tcggaagtag aagcatcagt agaccgattg
    66781 atcttacaaa aagaagaatt gacacaaaag caggcgcagc taaaatcatc tttaactgaa
    66841 acaaaagaga agttagaaat gatccaacaa aacgggaaaa agttccaaga agcattagca
    66901 aaagaacagc ctaaaatgta tcaattaatg aatcaagtcc agcagctacg tgctcgtaag
    66961 aaaagccttc aagaaatcca agaaaactat tttggttttt accaaggggt tcgattggtc
    67021 ttgcagcata aacagcagct ttcgggtatt gtcggagcag tggctgaact gatcgatgtt
    67081 cctgcagatt tcacgttagc tatcgaaaca gctttaggtg gggcagccca gcatgtcatc
    67141 gttgaaaatg aaaaagatgc tcggcaagcc atcacgtatt tgaaacagca aagaggagga
    67201 agagcaacat tccttccact gacgacaatc aaaccaagac aattaccggc tcatatattg
    67261 acacaagcag cagctgtaga agggtttata gggattgcca gtgaacaagt cagttatccc
    67321 gatcaaatcc aaactgtggt tcaaaacctg ctaggaacga tccttttagc caaagactta
    67381 actagtgcca atgcaatcgc gcaaacgatc cgctatcaat atcgagtggt ttccttagaa
    67441 ggtgatgtga tgaatgccgg aggatccatg acaggtggag cgaataaacg aggaaaccaa
    67501 ggcagtttgt ttgtacaaaa tcaagaattg aaacagttga ctagtgaatt tgaagaagca
    67561 gataaacaac tccaagcaca agaaaaaaaa gttcaggagc tgcaacaaga aacagctcgt
    67621 ttagccgaag aacaagaagt tttgcgtaca cgaggcgaac aattacgttt tgaagaacaa
    67681 gaagcgacaa atcagctaca aaacatcata aatgaacttg aacgttttga aaaagaaaag
    67741 caaatctcaa cttttgagac acgtgaactg cagcagttta tcgaagatta cgaaaagcaa
    67801 acgaacgaat tgaaagacaa acaaacagat cttgaatcgc aacgacaaaa aatcgacgaa
    67861 gaaatcaaat cgttaagtca agaaagcgac cagatggaag ccagaagagc acaagtacaa
    67921 agtcaaaaag cacaagaaca agcagatctt gctgtcttga aagagcagtt taatcatctg
    67981 cagatccagc tccgcggtgc ccgagtccaa aaagcagagg caaccgaacg tcaagaagcg
    68041 atagaaaagc agttggcgac attgacagca gacttttctg atcatgaagt cacagaagaa
    68101 agtttggaaa aacaaattaa cgaattatct gctcagcgag aaacattaaa agccgagttg
    68161 gcaaaagcca aagagcagcg agatagaagg caaaaggaga tcgataagct tgaagcagtc
    68221 ttggcagagc gtaatcgtga gcaaaaagca cgactgtcgg aacaatccaa attagaagtc
    68281 caaaaagacc gagctgaaat gattttagat aatcatttga cctatctgca gtcagaatat
    68341 cagctgacat ttgagaaagc ttcccaagac tatcaagaaa caaccgatat cgaagacagt
    68401 agaacaaagg tttcatcctt aaaagaacag attgaaaaac tgggaccagt aaacttaaat
    68461 tcaatcgaac aatacgaaca agtcagtgaa agacatacat tcttagcaac acagcgagat
    68521 gatctgttag cagcgaaaaa ccaattgttt gaaacgatgg atgagatgga tgatgaagtg
    68581 cggacaagat tcaaagaagt atttgaagct atccgtcaag aattcaaaat tgtctttcca
    68641 aatatgtttg gcggcggtaa agctgaatta gtactgacgg aaccaactga tttgctgaaa
    68701 acaggtattg agattgaagt acaacctcct ggcaaaaaac tgcaaagtct aagtttgctt
    68761 tctggaggag aacgagcact tacagccatc gcgttgctct tttccattat ccgtgtctgt
    68821 cctgttccgt tttgtatctt agatgaggta gaagcagcgt tagatgaagc caacgtgaaa
    68881 cgttttggtc gttacttgag cgatttccaa gatgacacac aatttatcgt agtcacacac
    68941 cgcaaaggaa cgatggtcgc agcagatgta ctgtacggag tgacgatgca ggaatccggt
    69001 gtatcaaaaa tcgtttctgt acggatggaa gatatcaatg aagaggcgaa aatcaacgaa
    69061 acgaaagtgt aggaacgaaa atgatcaaat tagtagcaat cgatttggac ggtacattat
    69121 taaatgaaca aaagctgatt tcagatgaga ataaacaagc attagcacaa gcaaaacagc
    69181 aaggggtcaa gatcgttcta tgtacaggac gccccttagc tgcgatggcg cattatctgc
    69241 aagaattagg actggtagat gaaggtgact acagcatcac tttcaacggc ggtttggtac
    69301 aaaaaaatga tactggtgaa atcatggaaa agaaagtgat ggaagtatca gatatccaac
    69361 gcttatacaa actggctcag caattggatc tgccactaga tgttttgtct gacaatgtgg
    69421 ttttacagct gcctagtgcc ccaaggaagc aatccttgta caatgtgttg aataagctgc
    69481 tgcaattcca accagcggat ataagcgata ttacagatga atttgtgctt aataaggcag
    69541 taatcgctta tgaccaagaa gaattagatc ctaaaatcaa agaaatacca gcagaattcc
    69601 atgaaatgta tgagatcatc aaaacaagaa gtatgctttt ggaatttatg ccaaaaggtg
    69661 ttacaaaagc ttatggtatt tctttattgg caaaagatct tggtttgaaa caagaagaaa
    69721 tcatggcgat cggtgatgag gaaaatgatt taccgatgat ccaatatgct ggactaggcg
    69781 tagcgatgga gaatgctgtg cctttcgtca aagaagcagc agatttcgtg acagccagca
    69841 atgaagaaaa tggcgtagcc aaagtgatcc aaaaatttgt attagagtag ggggagtcaa
    69901 atgggatttt ttgataaaat caagcgtgct tttactggcg aagagaaaac agaagaagtt
    69961 gaagtacagg agaagtacga caagggttta gaaaagacgc gtaaaacatt tggtgaacgg
    70021 atgaatgagt tgtttgccaa ttttcgaaca gtcgatgaag acttttttga agaattagaa
    70081 gaaacactta tcggtgcaga tgtaggtttt gaaacagcga tacgaattac cgaagcatta
    70141 cgtcaagaag tgaaactcag aaatgcgaaa aaatcggcag aagtccaaaa tgcgatcatc
    70201 gagaaacttg tggatctcta tgaagcggaa ggaatcaatg aagtaaatga actaaatatc
    70261 caagaagacg gactaacagt tattttgttt gtcggtgtta atggtgtagg gaaaacaaca
    70321 agtattggta aacttgccca tcagttcaaa aacgaaggta aaaaagtatt gatggcagca
    70381 gcagatacct tccgcgctgg agcaatcgac cagttagttg tctggggcga acgcgctggt
    70441 gttgaagttg ttcgtggcaa tgcaggaggc gatccagcag ctgtcgtatt cgatgctttg
    70501 gaaagagcaa agacagagca ggcagatgtt ttactagttg atactgcagg ccgactgcaa
    70561 aataaagtga acttgatgaa cgaattagaa aaaataaaac gagtaatcca gcgggaatta
    70621 ccagaagcac cccaagaaac acttcttgta ttagatgcaa ccactggtca aaacgcgatg
    70681 gttcaagcaa aacaattcaa agaaacaaca gatgtgaccg gtcttgtttt gacaaaatta
    70741 gatggtacag caaagggcgg tatcgtactt gctatccgta atgaattgca tttaccagtg
    70801 aaattggtcg gtcttggtga aggaatcgat gacttggagc cgtttgatcc aaatgatttt
    70861 gtcgttggat tgttcaaagg attactaaaa gaagaataac ccatgtctaa aaagcaggag
    70921 ctgtgacaaa agttgcagct ccttgtttga atgattgttt ttaacatagc tgtgtttttg
    70981 atgccttcat ttttatactt atttgtaagc ggttctcaaa caatttcgtt ctttttctta
    71041 gactttttga ttataattag attcatatga attggaggtc acttaatgat gaattttaag
    71101 caattattat tgcatgtcaa tgcgcgccct tttatcgacc aagctcgatt tggtatagaa
    71161 cgggaaggac agcgggttga tcttgcagga aatctagcaa aaaccgatca tccagcaatc
    71221 tttggcgatc gatcctatca cccctatatc caaacagatt ttagcgaaac acaaacagag
    71281 atgatcaccc ctgttaccga ttctattccc gaattatttc agtatctcgc tgctgtttat
    71341 gatgtgactg ctcgttctat accgaaagaa gagatgatct ggccattaag tatgccacct
    71401 gccttaccgg aaaaagacga agagatcatt attgcaaaat taaaaaattt cgaagatgtc
    71461 ttgtatcgac gttatttagc aaaagaatac gggaagcgaa aacagatggt aagcggtatt
    71521 catttcaatt ttgaatttgg cgatgagttg ctaagaacat tgttcagcca tcaggaagaa
    71581 tttcaagatt tttccgaatt caaaacagaa ctttatttga aaacagcgag aaactttatg
    71641 cgctatcgct ggatgatcac ttatttattc ggtgcttcac caatgagtga gaagaactac
    71701 tttttagatg aatcacatcc gcaagaacct gttcgcagta ttcgcaacag tgcgctgggc
    71761 tatacgaatc atccaaatgt gaaagtttcc tatgcttcta tgaaacagta tttagcagat
    71821 atcgagcgaa tgatcgaaga agggaaactt tcggaggaga aagaatttta tacaccgctt
    71881 cgtttccgag gcggaaagaa agtcgcagat ttagcaacaa caggtgttcg ttatattgaa
    71941 ttgcggaata tcgatttgaa tccttatgca agattgggta tcaatccaga gcaagttcgg
    72001 ttcttacaat tattcctgat gtacatgtta tggacagaag aaaaagaaga ctgtgaccaa
    72061 tgggtagcag aaggtacgac tcgaaataac aaagtagcac tagaacaacc atcagatcaa
    72121 acagaatttc atcaagaagg tagagaaatt cttgaaggaa tgaagcaaat gctggttgaa
    72181 ttggattggt tggattctct ttatttagta gaggaagcat tgactcaaat ggatcatcct
    72241 gaacaaacgt tagcagcgaa actctatcaa gaagcacagc tatcgagtca gcaagaagtt
    72301 gctgtggcat tgggacatca atattataaa gaaagccatg aacgtcccta tcagttggcc
    72361 ggttttcgcg aaatggaact ttctactcag atatttatgt tcgatgccat ccaaaaaggt
    72421 gtccaagtca aagtattaga tgagtcggat caatttttgc gtctgcaatt ccaagaccat
    72481 gtagaatatg tgaaaaatgc aaacatgaca agtaaggaca gctatatcgt cccacttatc
    72541 atggaaaaca aaacagtaac gaaaaaagtc ttaaaagaag ctgggttccg ggtaccagga
    72601 ggcgcagaat tttcatccat ggaagaagcg gtaaaagctt atccgagatt tgccgagcag
    72661 gcatttgtga tcaaacctaa atcaaccaat tacgggttag gtatcacgat tttcaaggaa
    72721 ggagctagtc tggaagatta tcaagcaggt ttagccattg cttttcgtga agacagttcg
    72781 gttttagtag aagaatttat gccgggaacg gaatatcgtt tctttgtgat cgacggagaa
    72841 gtccaagcca tcatgttgcg agtccctgct aatgttattg gtgacagtat ccgaacagtg
    72901 aaagaacttg ttgaagaaaa aaatagtgac cctttgcgtg gaacgaatca tcgtgcacct
    72961 ttagaattga tccaattagg tgaattagaa caattgatgt tgaaagaaca agggttgacg
    73021 atcgaatcag ttccccaagc caatcaaatt gtttatctaa gggagaactc aaatatcagt
    73081 acaggtggtg attcgatcga tatgactgat gaattttctg aagcttacaa aaaaatcgcc
    73141 gtttctgcgg tagaagcact aggagctaag attagtggta tcgatttgat tataccagat
    73201 aaagaaatcg atcctacaac tgataaaaaa gcatatggga tcatcgaagc aaatttcaat
    73261 ccagccatgc atatgcatgt ttatcctttt gcaggaaaag gcagaagatt gacgatgaat
    73321 gtattgaaat tgttatatcc tgaagtattt taaaagaaaa agcttttgac agattgtcct
    73381 atttgatgtc aatctgatca aaagcttttt ttgcatgtaa aaatttatgt gttttttcgt
    73441 tttaaaataa acgatcccag aagctttttt tcttttttgg tttttctttt tcagctggcg
    73501 aagagttttc ttctgggtat tttttttcga tatcaatcac aggttcattt actgcttctt
    73561 ttgctgttaa gagcaatccg aattcctcag gatttgcagt gacgtaatcg ttgactacag
    73621 tgaaacggct attgtttttc gttgctagct ggatatattg gttttggaca gaaaggggta
    73681 gagccccatt caagagtatg gaagcatctg gatgtttttt gagttctttt aaaaagtttt
    73741 ctttgttttt tggttggatc atttcagcaa cagtcatact aagatagcag cgttccctga
    73801 atgtccctaa atatttatgc tgttcttcag ggttcactaa aggtgtacca taaagtccct
    73861 tatccaaatg gctttttaca tcatcttgtg ccataaaaaa taccctcctt cttctctttg
    73921 tctttcttac gattatagca tattcccaat aatacctaaa acatatgaaa aagcggttgc
    73981 ttttgttcaa cgattcactt aaaataagaa tgaaaatcat tctcaattag aaaggtggat
    74041 tggatgcgct acggacgact agtagcatta ttagttattt taagtagtat ttcattgttc
    74101 atcggagtat cgaatgtaca attgtcagaa ctgctccagt tagatgggca aagcttacaa
    74161 atcttgctag tgactcgttt gccacgaacg atttgtttga tccttgtggg agcgaccagt
    74221 agtatttgtg ggttgatcat gcaacatttg acccaaaata aatttgtttc accaacgact
    74281 gcgggcacga tggatagtgc gcgtttaggt attttagtgg ctatgatttt cctacctggt
    74341 gcatccttga ttgtccgatc cttgacagca ttttgctttg cttttgcagg aacattgctt
    74401 tttttatccc ttacgcgatt gttcctccag aaaaatcaat tgatgcttcc gttgattgga
    74461 gtcatgttag ggaatatcat tggttcagtt gctacttttt ttgcttatca attccagttg
    74521 gtacaaaata tgacttcatg gcttcaaggg aattttgcga ctgtgatacg aggaaattat
    74581 gagttattgt atgctgttgt tccattgttg ctcttggttt ggctatttgc ttaccgattt
    74641 acgatcattg gtctgggcga atcgtttgca gtaaatctgg gaatagatta ccagaagatg
    74701 cagtttttag gcattggctt agtatcattg gctagtgcag tcatgttgat catggtagga
    74761 agcattcctt ttatcggagt gatcattccg aatcttgtct ctagaatata tggagatcaa
    74821 gtgcacaaaa caattggcgt aactgcttta gcaggtagtt tgttcttagt attttgcgat
    74881 cttgtcgctc gcgtgttgat ttttccttat gaaataccag tcagtgtgat cgttggaatc
    74941 atcggtgggg ctatcttcct ttatcttctg ataaggggac gacgatatgc ttaaaaaaaa
    75001 gccatctatt tttattggta tgcttgttct tgttttattt gttacaggaa tgtacttgac
    75061 gatccataca tatggaaact ggcattttgc atgtgcattg aggagtaaga aaatttttgc
    75121 atttatcctt gtctcgctag ctgtcagcag tgccacaatc agctttcaga caattacgca
    75181 taaccagttt ttgactcctt cagtattggg acttgagtcg ttatatgtcc ttgtccaaac
    75241 ggtccttttc ttctttatcg gaggagtgac gatgcttcag cagacaggta cagttttttt
    75301 cttattgaac gttgggctga tggtcgggct gagtctattg ttatttttct ttcttttgaa
    75361 aaaaatgaga gcgaatcttt ttttattact gatgtcaggg atgattttag gtacgctttt
    75421 tggtagtgtc agtacctttt tacaagtcat catggatccg aatgaatatg atttgcttca
    75481 aggcagatta tttgcaagct ttggcaatat ccagagtcag tatcttttgc ctgcttcttt
    75541 gatcattatc ggctgctggt acctgttatt tcgatatcga catgtgctag acgttttcca
    75601 tctcggaaat gaacaagcag taaacctcgg gatagacaca gatcgttttg aatttttgat
    75661 tttatgttgt gtcagtcttt tgacagggac atcaacggct cttgtgggtc cagttacttt
    75721 tttagggttt attgtcgcaa atatcagtta tcgtgtcttt tctacttatc gtcatggtca
    75781 gctatttatc ggaagctttc tggtagctat gctgtttctg acaggaggac agctcgcagt
    75841 agagcagatt ttcggctgga atacaacagt gagtgtcgtc gttgaatttg tcggaggaat
    75901 ttattttgtc acaaaattga ttcgcgaaag gaagagtatc ctatgattga tttacaaaag
    75961 gtcaataaac tgtaccatgg aaaatcagtg gttaaagatg tacagttaac gattcctgaa
    76021 aagaaattga ctgcttttat cggtccaaat ggtgctggaa agtcaacggt gttgtcgatg
    76081 atcagtcgtt tgataccaaa agatacaggt gaaatctatt tagaccataa cgaagtaaag
    76141 gcttggaaat ccaatgaatt agccaaaaaa ttatcgatcc tacgacaatc gaatgaaata
    76201 aatgtccaaa tcaccgtgaa agaattagta gaatttggac gatttcctta tacgaaagga
    76261 aaattgacga caaaagacga ggaaatcgtt caggaatcac tggaacacct tgggttagaa
    76321 gaaatagcgg atcaatcgat tgacactctt tcaggcggtc aactccaacg tgcgtatatt
    76381 gcaatggtgc tagcgcaaga cacggattat attttactgg atgaaccttt gaacaatcta
    76441 gatatgaatt ttgcggttca aatcatgcag attttaaaaa atttagtaca agaaatcggg
    76501 aaaacaattg ttattgttct tcatgatatc aactttgctg caagttatgc agacgaaatt
    76561 gttgcaatga aagatggaca gttatttacg caaggaacga cccaagaaat gataaaaaaa
    76621 gatgtattgg atgaattata cgaaatggac atacggattt gtgagattga aggccgcaga
    76681 ttctgtcttt atttctaaaa ctaacattag gagagaagag atgaaaaaat tagtagtagg
    76741 tatcttgatt ctagcatcat ttggtttagc agcatgtgga aatagcaaca acaccagtca
    76801 ggcagacaca aaaaaaagca gcacacaaac tgaaactaca ttgacgataa cagatagtaa
    76861 tggagaccaa atcgaggtgc caaataatcc agaaaaagtc gtcgtattcg acaatggttc
    76921 tttagatacg atggatgctc ttggtgttgg agataaggta gttggagcag ctacaagcag
    76981 ccttcctgag tatctttcat cctataaaaa agtagaatca gcaggtggaa taaaagaacc
    77041 agacttagaa aaaattaatc agcttcagcc agatctaatc atcatttctg gccgacaaag
    77101 agattttcaa aaagatctaa gtcaaattgc tccgaccatt ttcctttctt tagatgcgaa
    77161 aaatccatgg gaatctttcc agcaaaatgt cactgcttta ggggagatct ttggtaagca
    77221 agaagaagct aaaactcaat tagaggaatt atctagtgca atcgatcaga cgaaaaagaa
    77281 agcagaagct actgacaaaa aagcactagt cacacttgtg aatgaagggc aattatcagc
    77341 ctacggatct ggttctagat tcggtttcat ccatgattta tttggctttg aacaagcaga
    77401 tgatcaaatc gaagcatcca ctcacggtca aagtgtttct tatgaatatg tgctggaaaa
    77461 aaatccggat atcttatttg tagtagacag aacaaaagct attggtggag acgacagcaa
    77521 agatgatatt tctgccaatg aactggtagc acaaacgaat gctggtaaaa atcagcagat
    77581 catctccctt gaaccagatg tctggtattt aagcgggggc ggtttagaat caatgaaact
    77641 gatgatcgaa gatgtgaatc aagcatttaa atagcataaa taacagagga ttaaactctc
    77701 atactatgta acaataatgt ttcttacatt cttaaatagc aagagcctat cctcaaagaa
    77761 gttatcaaaa accatccgta aaatcgtatt gatgatttta cggatggttt ttatttattt
    77821 gagaaatctt ttttcaacgt gaattcatta tctacatcat acgggttgat tctagaatgg
    77881 ggagtggcaa aaaatagtgt gatccatttt ttcagcatca agatcgcctc ctttgatttg
    77941 aatcaaaggt acaaagcgaa acttagataa aacttaaatt tagatatttt tattttgtac
    78001 cgatctcttc tttaaacgat gttccatctg ctgcttctcg gcaataagtc cactagacta
    78061 gaaaatatga gaagctattt tctgtccagc gttcttatta ctcaaagcaa atcgcagttg
    78121 tcacaatctc ttctaaacga tgttccatct gctgcttctc ggcaataagc ccactagact
    78181 agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaagca aatcgcagtt
    78241 gtcacaatct cttctaaaca atgttcaatc agctgacctt cggtattagc tcaaagcact
    78301 gatttcacaa cctctattta ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc
    78361 atttttcgat aaaaatcggc gggaagctta ctccaccgtt ttaataattg atagaaataa
    78421 ctctcttctg ctttgatttt atactcgctt aatatcaatc caaaactatt cgtttttttt
    78481 gaagctaatt ttttataaat atccaatgct ttggttttat cttcattgac aaagagtgtt
    78541 tttacttcaa tataagaacc ctcgatattt tgtgtttctt cgataaaata gatgtttttt
    78601 atatattcct tcatagtgat tcctccaaag aagtctttta tttgataagt ttactctacc
    78661 gaagagttgt taataatccg tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt
    78721 tttttatttt ttatagtatt ttttgttatt taagaaagga ataaggttaa acaaataaca
    78781 ggttgctttt tgggtctttt tccgttcaag aaactgaacg aagaaaaggg aaagttgtct
    78841 cttttgtgat aagagaaatc ataataaacg aatgacttac tttccaataa attgagaaca
    78901 gtcagaacca gacaaattag ttgcatctga tgaataaagt gattattcta tgacatgagg
    78961 tgatacagat gaaattagaa gtgacaaaag aagcacaaga agtattgaag aataaattag
    79021 aagcagacga tcaactttta ttagatatcg aaaacggtga cggtccattt gcagaaagcc
    79081 aagtcagttg ccaattggat acggcatttc gattgatcat tgtcaaaaaa gatacacaaa
    79141 cagatctaag tctttattct gtagtggctg atacaccagt tggaccaatc aaaatcaaag
    79201 acagtgcaat actttacatg gatgatccat ccacacttgc tttagaacca acttataata
    79261 gtttacaatt aaaaggacca agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag
    79321 actagaggga taaaatggga aaattggcga tcatcagcga cctacatgtg gacatcaata
    79381 aattcggcga atcagagctg atgcagcttt ggcaagtttt gcaaaagcac aagatcactc
    79441 ggcttcatct agccggagat acggctaata aagtagaccg ttgcctagct gttgtcgaat
    79501 ttttcgtcaa taaaggatta tctacgacat ttcatttcgg caatcacgag ttggcagatg
    79561 tgcccaatga acagatgatg gaccattttc cagacccaca ttttttaaat gaacggtata
    79621 ttgctttaaa tgagcagacc gttcttttag ggatgaatgg ctggtacgac tatcagtttt
    79681 ccgaattgaa agattacgag gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt
    79741 tcatccagcg tgggaaaaca gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa
    79801 atttgctaga ccagctggaa gcaaaaaaac ttgaggtgat tttatccact catttcgttc
    79861 ctaaacgggc atttatcatg tatcaatctg ctccttatga gcgttggaac aaattaaatg
    79921 catttttagg atcagaatca ttcggagaat tacttgaccg ttattcaaat atcaaacaag
    79981 tagtttttgg ccatacgcat cgtcgctttg aagataaatc catcggtcac acgatttaca
    80041 gctgcagacc ttttggttat tattatgaat ggcagctgac ccgagatttt gtcttgaaag
    80101 aacagttgct agacagctat gatcctatga aattacgtgt cctcttgcgt gcccatcacc
    80161 cagcttttca ggcctatcaa acacaacatt tgcaagagga atttgaacaa gcgatgacaa
    80221 tcgttccgta ttgagacagc attagtaatg tgtaaaagaa aataccttgt aaaacataag
    80281 gataagtaag agcagtagat ccatttatcg tggatatgct gctttttttg cggaaaataa
    80341 tcagcgaatg tttatttgat cggatgattt gtaactcgca gagtactaga tgcaagcgtg
    80401 tattcttttt ttgaacgata atcacaaaaa aaaatata
//
LOCUS       DAJSBM010000006        72629 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJSBM010000006 DAJSBM010000000
VERSION     DAJSBM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.006"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..791)
                     /locus_tag="ONT67_000512"
     CDS             complement(<1..791)
                     /locus_tag="ONT67_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2354275.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYDQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGK"
     gene            966..1490
                     /locus_tag="ONT67_000513"
     CDS             966..1490
                     /locus_tag="ONT67_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR2354276.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            1506..3005
                     /locus_tag="ONT67_000514"
     CDS             1506..3005
                     /locus_tag="ONT67_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR2354277.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3093..3695)
                     /locus_tag="ONT67_000515"
     CDS             complement(3093..3695)
                     /locus_tag="ONT67_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2354278.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            3799..4755
                     /locus_tag="ONT67_000516"
     CDS             3799..4755
                     /locus_tag="ONT67_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCR2354279.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(5060..6016)
                     /locus_tag="ONT67_000517"
     CDS             complement(5060..6016)
                     /locus_tag="ONT67_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2354280.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            6190..6750
                     /locus_tag="ONT67_000518"
     CDS             6190..6750
                     /locus_tag="ONT67_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354281.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            6743..7567
                     /locus_tag="ONT67_000519"
     CDS             6743..7567
                     /locus_tag="ONT67_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR2354282.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            7577..8194
                     /locus_tag="ONT67_000520"
     CDS             7577..8194
                     /locus_tag="ONT67_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2354283.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            8212..8895
                     /locus_tag="ONT67_000521"
     CDS             8212..8895
                     /locus_tag="ONT67_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR2354284.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            9578..10813
                     /locus_tag="ONT67_000522"
                     /pseudo
     CDS             9578..10813
                     /locus_tag="ONT67_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            10821..11708
                     /locus_tag="ONT67_000523"
     CDS             10821..11708
                     /locus_tag="ONT67_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354285.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            11725..12561
                     /locus_tag="ONT67_000524"
     CDS             11725..12561
                     /locus_tag="ONT67_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2354286.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            12564..13187
                     /locus_tag="ONT67_000525"
     CDS             12564..13187
                     /locus_tag="ONT67_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354287.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            13184..14476
                     /locus_tag="ONT67_000526"
     CDS             13184..14476
                     /locus_tag="ONT67_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2354288.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      14835..14932
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            15048..15629
                     /locus_tag="ONT67_000527"
     CDS             15048..15629
                     /locus_tag="ONT67_000527"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR2354289.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            15632..16939
                     /locus_tag="ONT67_000528"
     CDS             15632..16939
                     /locus_tag="ONT67_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCR2354290.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            17232..17720
                     /gene="purE"
                     /locus_tag="ONT67_000529"
     CDS             17232..17720
                     /gene="purE"
                     /locus_tag="ONT67_000529"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR2354291.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            17713..18837
                     /gene="purK"
                     /locus_tag="ONT67_000530"
     CDS             17713..18837
                     /gene="purK"
                     /locus_tag="ONT67_000530"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR2354292.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            18850..20145
                     /gene="purB"
                     /locus_tag="ONT67_000531"
     CDS             18850..20145
                     /gene="purB"
                     /locus_tag="ONT67_000531"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR2354293.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(20325..20702)
                     /locus_tag="ONT67_000532"
     CDS             complement(20325..20702)
                     /locus_tag="ONT67_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354294.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            20958..21686
                     /locus_tag="ONT67_000533"
     CDS             20958..21686
                     /locus_tag="ONT67_000533"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR2354295.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            21686..21940
                     /gene="purS"
                     /locus_tag="ONT67_000534"
     CDS             21686..21940
                     /gene="purS"
                     /locus_tag="ONT67_000534"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCR2354296.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            21942..22613
                     /gene="purQ"
                     /locus_tag="ONT67_000535"
     CDS             21942..22613
                     /gene="purQ"
                     /locus_tag="ONT67_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCR2354297.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            22614..24836
                     /gene="purL"
                     /locus_tag="ONT67_000536"
     CDS             22614..24836
                     /gene="purL"
                     /locus_tag="ONT67_000536"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCR2354298.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            24821..26260
                     /locus_tag="ONT67_000537"
     CDS             24821..26260
                     /locus_tag="ONT67_000537"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR2354299.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            26283..27335
                     /locus_tag="ONT67_000538"
     CDS             26283..27335
                     /locus_tag="ONT67_000538"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR2354300.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            27332..27910
                     /gene="purN"
                     /locus_tag="ONT67_000539"
     CDS             27332..27910
                     /gene="purN"
                     /locus_tag="ONT67_000539"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR2354301.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            27907..29442
                     /gene="purH"
                     /locus_tag="ONT67_000540"
     CDS             27907..29442
                     /gene="purH"
                     /locus_tag="ONT67_000540"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR2354302.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            29458..30732
                     /gene="purD"
                     /locus_tag="ONT67_000541"
     CDS             29458..30732
                     /gene="purD"
                     /locus_tag="ONT67_000541"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR2354303.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            30854..31768
                     /locus_tag="ONT67_000542"
     CDS             30854..31768
                     /locus_tag="ONT67_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2354304.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(31901..33466)
                     /locus_tag="ONT67_000543"
     CDS             complement(31901..33466)
                     /locus_tag="ONT67_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2354305.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(33459..34910)
                     /locus_tag="ONT67_000544"
     CDS             complement(33459..34910)
                     /locus_tag="ONT67_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2354306.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(34924..35940)
                     /locus_tag="ONT67_000545"
     CDS             complement(34924..35940)
                     /locus_tag="ONT67_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2354307.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            36160..37248
                     /locus_tag="ONT67_000546"
     CDS             36160..37248
                     /locus_tag="ONT67_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCR2354308.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            37302..38828
                     /locus_tag="ONT67_000547"
     CDS             37302..38828
                     /locus_tag="ONT67_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR2354309.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            38830..40035
                     /locus_tag="ONT67_000548"
     CDS             38830..40035
                     /locus_tag="ONT67_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354310.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            40292..41269
                     /gene="guaC"
                     /locus_tag="ONT67_000549"
     CDS             40292..41269
                     /gene="guaC"
                     /locus_tag="ONT67_000549"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR2354311.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            41533..42957
                     /locus_tag="ONT67_000550"
     CDS             41533..42957
                     /locus_tag="ONT67_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR2354312.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT67_000551"
     CDS             43418..44527
                     /gene="pdhA"
                     /locus_tag="ONT67_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCR2354313.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            44530..45507
                     /locus_tag="ONT67_000552"
     CDS             44530..45507
                     /locus_tag="ONT67_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCR2354314.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            45532..47175
                     /locus_tag="ONT67_000553"
     CDS             45532..47175
                     /locus_tag="ONT67_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR2354315.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT67_000554"
     CDS             47183..48589
                     /gene="lpdA"
                     /locus_tag="ONT67_000554"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR2354316.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            48751..49026
                     /locus_tag="ONT67_000555"
     CDS             48751..49026
                     /locus_tag="ONT67_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCR2354317.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            49032..49799
                     /locus_tag="ONT67_000556"
     CDS             49032..49799
                     /locus_tag="ONT67_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCR2354318.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            49967..51796
                     /gene="typA"
                     /locus_tag="ONT67_000557"
     CDS             49967..51796
                     /gene="typA"
                     /locus_tag="ONT67_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCR2354319.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            52057..53829
                     /gene="recQ"
                     /locus_tag="ONT67_000558"
     CDS             52057..53829
                     /gene="recQ"
                     /locus_tag="ONT67_000558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCR2354320.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            53943..54245
                     /locus_tag="ONT67_000559"
     CDS             53943..54245
                     /locus_tag="ONT67_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCR2354321.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            54248..55411
                     /locus_tag="ONT67_000560"
     CDS             54248..55411
                     /locus_tag="ONT67_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2354322.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            55437..58865
                     /locus_tag="ONT67_000561"
     CDS             55437..58865
                     /locus_tag="ONT67_000561"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCR2354323.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            58886..60010
                     /locus_tag="ONT67_000562"
     CDS             58886..60010
                     /locus_tag="ONT67_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354324.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            60000..60437
                     /locus_tag="ONT67_000563"
     CDS             60000..60437
                     /locus_tag="ONT67_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2354325.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            60421..60759
                     /locus_tag="ONT67_000564"
     CDS             60421..60759
                     /locus_tag="ONT67_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCR2354326.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT67_000565"
     CDS             60878..61438
                     /gene="rsmD"
                     /locus_tag="ONT67_000565"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCR2354327.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            61435..61926
                     /gene="coaD"
                     /locus_tag="ONT67_000566"
     CDS             61435..61926
                     /gene="coaD"
                     /locus_tag="ONT67_000566"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR2354328.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            61916..62959
                     /locus_tag="ONT67_000567"
     CDS             61916..62959
                     /locus_tag="ONT67_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCR2354329.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            63118..63846
                     /locus_tag="ONT67_000568"
     CDS             63118..63846
                     /locus_tag="ONT67_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR2354330.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            63962..64207
                     /locus_tag="ONT67_000569"
     CDS             63962..64207
                     /locus_tag="ONT67_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2354331.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            64204..64422
                     /locus_tag="ONT67_000570"
     CDS             64204..64422
                     /locus_tag="ONT67_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR2354332.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            64558..65097
                     /locus_tag="ONT67_000571"
     CDS             64558..65097
                     /locus_tag="ONT67_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCR2354333.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            65090..67363
                     /locus_tag="ONT67_000572"
     CDS             65090..67363
                     /locus_tag="ONT67_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCR2354334.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            67401..68447
                     /gene="holA"
                     /locus_tag="ONT67_000573"
     CDS             67401..68447
                     /gene="holA"
                     /locus_tag="ONT67_000573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR2354335.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(68536..68790)
                     /locus_tag="ONT67_000574"
     CDS             complement(68536..68790)
                     /locus_tag="ONT67_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR2354336.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(69190..69459)
                     /locus_tag="ONT67_000575"
     CDS             complement(69190..69459)
                     /locus_tag="ONT67_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354337.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(69929..70384)
                     /locus_tag="ONT67_000576"
     CDS             complement(69929..70384)
                     /locus_tag="ONT67_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR2354338.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(70522..72450)
                     /locus_tag="ONT67_000577"
     CDS             complement(70522..72450)
                     /locus_tag="ONT67_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2354339.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        23651 a        12522 c        15835 g        20621 t
ORIGIN      
        1 ttttttccta cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt
       61 gtattgatca agaggatagt tttatcagaa gagatggcta agattccttc atctacttca
      121 ttttcgataa tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc
      181 agtacttttt tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca
      241 ataatcaatg aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg
      301 tttgttaagc caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa
      361 tagcggaagc cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc
      421 acttttttca catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga
      481 gaatatcgaa tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg
      541 atttcatttg ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg
      601 atgagtttat cattggcaac gattttagca atgctagaga ttttatctga cacactagat
      661 cggttcatcg aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat
      721 gtggagaaac aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa
      781 aaaggaaaca taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa
      841 atttaacaaa ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta
      901 aaagaagcta tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg
      961 tgtatatgat agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg
     1021 caggattggc agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg
     1081 atccgatgct tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc
     1141 gtttaacaga tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt
     1201 tggtattttt gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt
     1261 cgtcttctga aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac
     1321 gaggtatggg agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt
     1381 tttacagccc ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca
     1441 aggaaagaat ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt
     1501 gttaaatgga aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga
     1561 tgttgctgat cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca
     1621 tcagtggaag ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca
     1681 aaattgtgcg tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca
     1741 gtctattatc gagtaaattg gacgtctcca cccctgaagt gtataccgaa caaggcgtac
     1801 cggtgatgtg tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta
     1861 cagcagcaga acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag
     1921 tattagaagg acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa
     1981 atcgcgataa gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg
     2041 ggttgatcat cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact
     2101 ctttagggaa accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag
     2161 ctctaaaaga aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat
     2221 tacagcgaca aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct
     2281 acaaacaaga acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg
     2341 cccgagcaca acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgccaaa
     2401 aacaaatcga actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag
     2461 tgaagaaaaa agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag
     2521 tcaaagaagt gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg
     2581 aagccaataa gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa
     2641 aagcagaagc cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag
     2701 ctgtcttgag tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc
     2761 taggaaatat cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg
     2821 gagcgaaccg agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa
     2881 aagcgacaac aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta
     2941 ctagccaagt aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag
     3001 aataaagtag tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc
     3061 tctccatttt tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt
     3121 tggatcttag cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt
     3181 tcgaccaaat cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa
     3241 ttgataccga ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac
     3301 aaagcggatt cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa
     3361 atgataccgg aattattgtt aataccatct gacacgacga tcacaataat aactagaata
     3421 ggaagcaaga tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa
     3481 atgatagcta atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg
     3541 gttgtagtca agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt
     3601 cctcgttgag tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc
     3661 gtacgcatac ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt
     3721 actatcatac ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa
     3781 taaaaaaggg ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc
     3841 ttcttttcga tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc
     3901 attgcattat taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga
     3961 atcgcagcct ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat
     4021 actatttttg gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa
     4081 ttaaccgaag ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac
     4141 atgtcttggc agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct
     4201 ttgcttgcaa atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag
     4261 atcgaaaaag aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat
     4321 aaagaagatg tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa
     4381 gatgccagag aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac
     4441 ttctctatga gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga
     4501 atccagcaac cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa
     4561 acattttcag agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta
     4621 ttgtatcggc aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg
     4681 ttccagtttt tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa
     4741 aaaatccaaa aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac
     4801 aacctcttct ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa
     4861 caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct
     4921 cacaacctct ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa
     4981 acgaaaaata ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct
     5041 ttttcacaac cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt
     5101 cgatgacatc ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta
     5161 ccatattctt tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct
     5221 ttgtaccatc tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca
     5281 cataaccgtt ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc
     5341 tgacagcagc aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc
     5401 ctgtttcaaa aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac
     5461 gagcgaaaga tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat
     5521 tgaagaaccg gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt
     5581 ttaaagcccc cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt
     5641 tgaagattgt catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt
     5701 cagccagttc ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt
     5761 ccaatgctgc caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt
     5821 cgatttcagc atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac
     5881 ggtgagcgaa gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg
     5941 tctcttgatt gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc
     6001 attgatattt tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct
     6061 attattataa caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat
     6121 gaaagcagcg atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag
     6181 gggataaacg tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt
     6241 gaaactgaaa aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa
     6301 tggaaagatg gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc
     6361 actgattatg aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa
     6421 aaacgaaacg ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg
     6481 cttagcagca aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt
     6541 agataccttg acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg
     6601 aattttcgtc tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc
     6661 gattgggtga atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa
     6721 gaacaacgaa aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat
     6781 gatcgcagca atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa
     6841 tcaagcagtc gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac
     6901 aaccagcagc aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga
     6961 tgttaaaaca gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga
     7021 aattgatgag tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat
     7081 tgctccagaa tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat
     7141 ggtctcagct taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga
     7201 tgtgatgagt cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat
     7261 tacggagcca ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa
     7321 ctggtacaac agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc
     7381 taaatggatc gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca
     7441 cgaagatata actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa
     7501 cgctgagtat atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga
     7561 aaagtaggaa ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg
     7621 ttggccagca gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa
     7681 gaatctgtct tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca
     7741 atttattgaa cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc
     7801 tagtatcatt ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag
     7861 caaatacaat aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga
     7921 aacaaaggaa tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga
     7981 ttcatgggaa cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa
     8041 tcctgcgctg tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt
     8101 gcaagatgca ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga
     8161 gacgtatcaa ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa
     8221 caatttacgc cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc
     8281 attttagaag caagcgggat caagatattt ggaaaattga tccgttcgat catctttact
     8341 acggatattg cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt
     8401 acacctcatc cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc
     8461 ggtgtaggtg gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa
     8521 gcgcaaggtt cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa
     8581 gtttgtcgag tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata
     8641 gatgaaaagt tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca
     8701 gaagcagtac gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt
     8761 ccaacggaag aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg
     8821 ccaccaagta gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa
     8881 aagaatcatg aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc
     8941 tcgtcttttg tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt
     9001 ttttttacct gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa
     9061 caaaaaaatg tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta
     9121 aaaaaggcac ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga
     9181 actgaccaat caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat
     9241 ttcatacaga attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca
     9301 tgcttattta gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt
     9361 aattatcctc ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact
     9421 atctaatttt tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct
     9481 tggtttttat tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt
     9541 atattgaaaa ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat
     9601 cttagcttta agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa
     9661 aatcacgctg aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt
     9721 aatcgagctt tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta
     9781 tgaaggatat tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt
     9841 ccaactgaac aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt
     9901 ggagcaaggg ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta
     9961 cgacgggcaa ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa
    10021 aaatatattt gaacaattaa atatccctga accagcttca aattggacat gggatgaatt
    10081 tgaacaaatc agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga
    10141 agattttata gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta
    10201 taatcgggaa gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt
    10261 tttccagctg cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc
    10321 tgcaagtacg acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt
    10381 tctatcaagt aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca
    10441 gttgattaat ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc
    10501 gcaaatgctg agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga
    10561 tttctttcaa aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat
    10621 catgtcgaat attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt
    10681 tgattattta gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc
    10741 tttgaatccg gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga
    10801 agtaacagca gaaggaaggc atgaaaaaag aaaaactaaa acggttcttg gatcaagaca
    10861 atgtggtcgg ttacgtattt gcttctccat tcatcctagg atttctattg ataacagtga
    10921 taccgatgat cttgtcactt tattattcgt tcaccgatta tcgtttggga aatccagtct
    10981 catggatcgg cttggataat tatataaaga tgctgagtga tgcccgtttt ttgaactcca
    11041 tccgagtcac ttttctctat gtatttgcat ctgtacctgt gaaactgatc tttggtctat
    11101 tgattgctta tcttttaacg aggaatatca aaggaacgac tttctttcgt tctttgtatt
    11161 atgtgcctag tttgatcggt ggcagtattt ctatcgctct agtttggcgg gagttgtttt
    11221 ctaaaaacgg gttagtgaat attttcctgg aacgattagg cgcacctgcg ctttcctggt
    11281 ttagcgatca aaaactggtc atgatccctc ttgttttgat gtctgcttgg caattcggtt
    11341 cggcaatgat catctttgct gcaggtatca aagagatccc aggctcgtat tatgaagcgg
    11401 cagagatcga tggagcgaac aaatggcaga cgttttacaa gatcacgtta cctcttcttt
    11461 cgccgatcat tttgtataac ttggttatgc agacaatcgc ggcgtttatg acttttacgc
    11521 aggcatttat cattacccaa ggtggtccga atgatgccac gaacttttat gctctttatg
    11581 tgtataacca agctttttct tttaattcta tggggtatgc tagtgctatt tcgtggatca
    11641 tgctgttgat tatgggagct gtcacctttc tgattttcaa atcatcaaaa aaatgggtcg
    11701 ttgtgtgaat gagaggagga agaaatgaat aatacagtaa cgaaatggtt tcaatacggt
    11761 tttattttgc tattaggtct agtcatgatt tatccggtct tatggatgat cgcaggttct
    11821 tttaaaacgg atcaagagat tatgagtggt tcgctgaatt tgattccaga gacttttcaa
    11881 tgggaaaatt acgcaaaagg atgggcgggg tttgctggaa tttcattttt tactttcttt
    11941 aggaattcgt ttgtcatctc tgcagtttcg acgataggga cagttctgag ctcgacttgt
    12001 attgcttacg cattatcgag aatcgatttc aaaggaaaaa gattctggtt cgtcgtcatg
    12061 ttagtaacga tgatgattcc tgcacaagtg attttgattc cgcagtttat tatttacaat
    12121 cggctgaatt tggttgggac gtatgtgccg ctgattttac cacatttttt tggacaagca
    12181 tttttcattt accagatcat gcaattcatg gtcagcatcc ccaaagagtt agatgaatca
    12241 gcgattattg acggatgcag caagtatagt gtctttacca aaattatttt tcctttgctg
    12301 aaaccttcga tcatcacgac gatcatcatc cagttttatt ggaaatggga cgacttcatg
    12361 ggaccattga tttatttaaa caagccgaga tcttacacgg tatcgattgc catcaaatta
    12421 tttgcagacg ctggatctag tacgacttac gcgagtatgt ttgcgatgtc tacgttgtcg
    12481 ctgctaccgg tttttctgat tttcttattc ttcaataaat atcttgtcca ggggatcagt
    12541 acaagcggtc tgaagggctg aatatgaaaa agccaataga agagatgttt ctttacaagc
    12601 tcaactacta tgtctgtcgg atttttttaa ccagtcttat cagtaatgct cttttcgctt
    12661 tagttctagg atgtttgttc actttaggat tagtgctgtc tgctcgtgtt gtctatctga
    12721 tttatttttt gatgatgtat ccttcttatt atctatctgt ccattacttg gcagaaaaaa
    12781 aggtatgttg gaggaaaggg aaaatcaaac ggatgtgttt catcattacg ttgtttcagc
    12841 tgtttgtcat tatttttgca gttgattggt atttattagt gggaaagatt ggagcttggt
    12901 atctgattcc ttttctattg tgtctgttgc tttttgtatc ccatagtttt tttagtacgc
    12961 taattgatat ctgcaaagaa gataaagcag atagtcttgt ttatttgatc aaaaggaata
    13021 tctggctgtt gtcaaaaaac gtcaaggggt ttctgtggta tttatgcctt ttgtatggac
    13081 tgttctggtg tatacagcct gattttttac tcactgcatt actgtatcca gggctgccat
    13141 ttgtcacagt attttgctta aaagaaaagg agaatgactt taaatgaaga aaaaatatgt
    13201 acaagtcgga cttggtggga gagcgcgttt tttttatgag gcaattgcct ctacgtatca
    13261 tcaaacttgt gaactagtcg gtttttgtga tgtatctcaa acaaggatgg attatgcaaa
    13321 tcggatactt gtgaacaagt atggacagaa aaaagtaaag acatgtatgc catcacaatt
    13381 tgagcaacta ctggatgacg tacggccggc tgaagtcatt attacgtcag tagatcgaac
    13441 gcatcatgat tatatcattc gtgcaatgaa aaaaggatat gatgttattt gcgaaaaacc
    13501 tctgactatc gatgaggaaa aagctcaaga aataataacg gtccaaaaac agacgggaag
    13561 aagcctgcga gtcactttta attatcgtta tgcaccgtat cacacgaaga taaaggaact
    13621 gatagaaagt gatgtgattg gtgatgtgtt ttctatccac ttcgaatggc tgttaaatac
    13681 gcaacacggg gcagattatt tcagaagatg gcatcgaaat aagcacaata gcggcgggct
    13741 tcttgttcat aaatctaccc atcattttga tctagtgaat ttttggttga acagccgtcc
    13801 ccaaaaggta tttgcattcg gtggattaaa cttttatggg atgcataatg cggagaaacg
    13861 cggagtagaa atattttaca gtcgatgcta tggaagcgaa ctagctaaag ctgatccatt
    13921 tgctatagac ctgtcagcca atgaggaatt gcgagaaatg tatttagaag cagaaaaaga
    13981 cagtggatat attcgtgacc gcagtgtatt ttccgatgat atctctattg aagatacact
    14041 aggcgtaacg gtcaaatatg aaactggtgc gattttgacg tattcgctaa acgcgtatat
    14101 gccgtgggaa ggattcaatg tttctttcaa tggcagcaaa ggacggatcg aaatgaaagt
    14161 gatcgaaaag tcttacatca atgcaggtgg agataaaaag gatgaaggag cagccgttct
    14221 tcagtccatc catgtctatc cgatgttcgg tgagccatat gaggtaatcg tcgaacaaga
    14281 agcgggcgga catggcggag gagatccgcg tttattagat gatctatttg gtgaagaaaa
    14341 agaagaagat ccgtggaatc gggcagcaac tcacgtagat ggtattttgt cgattttgac
    14401 aggaatcgca gcaaatcatt ctattgcttc aggaaaagca gtagtgattg acgagcttgt
    14461 cagcttaaaa gaataagaat agatttacgg aaattacttc ccgtagttta taaaatcttg
    14521 ccttattgcc gataggctgt cttttgaaca ctatttattc ggaaaaaggg gctatgacat
    14581 gctttttgtc atgccttctt tttatgtggg ctgctttttt agataggaga aaataaaaat
    14641 aactatttaa acccgaacaa aaaggaataa tgcattaaaa atcattagat tttacaaaaa
    14701 aataagtaaa ccacgttgtc agtttcagaa agtagtgtta gactgttttc aatatttgaa
    14761 acaacatttt ttattcaaat atggcaaact gccttcacgt gcaaaacagt tgcaagaaaa
    14821 tgaatttaca ctattattag ttttacatac ctacttatat atcgtcataa tatggatgac
    14881 agtttctagc cagtaccgta aatgctggac tataagtaaa agattggcta tttcaccctt
    14941 cttttcttat gataaagtgg agggtgtttt tctttaacag aaacatagta ggtttttgca
    15001 caaaagcggt tatttcggta ttaataaatg ataaaggagc aaataaagtg aaagaattag
    15061 ttgaacgtat tcaaaaagac ggacgtatat taggcgaggg cgtattaaaa gtcgacagct
    15121 tcgtcactca ccaagtagac cctgtattga tggaacaaat gggaaaacgg tttgcagaag
    15181 tatttaaaga acaaggaatc acgaaagttg tgaccattga agcttctggg atcgcaccag
    15241 ctttatttgc tgcaagagaa ttagatgttc caatgatctt cgctagaaaa gcaaaaagtt
    15301 tgacgatgga tgaagaatta ttgacagctt ctgtttattc ttttacgaaa caagtgacaa
    15361 gtacgatttc tatttctcgt aaatttttat cttcagaaga caaagtattg attatcgacg
    15421 atttcttggc aaacggacaa gcggcaaaag gattgatcga attatgccag caagctggag
    15481 cgcaagtaga aggaatcggg atcgtcatcg aaaagtcttt ccaagatgga cgtcagttac
    15541 ttgaagaaat gggattgaga gtcgtttctc ttgcgcgtat tgcttccttg acgaatggac
    15601 aagttgagtt tcttgaggag gacgcataat cgtggaaaaa gaaacacaaa atggaaaagc
    15661 tgcggtttta gggctgcagc atctgctagc gatgtatgca ggagcagtag cggtgcctct
    15721 tttgatcggt acaggactag gctttaatca agaacagatg acttatttga tttcaattga
    15781 tatctttatg tgtggattag cgacattatt gcaattgaca gttaaccgat ttttcggaat
    15841 tggcttgcct gtcgttttag gatgtgcgat ccaagcagta gctccactga tcttgatcgg
    15901 aaccaatgaa ggagtaggag ctatctacgg atctattatc gcttctggga tctttgttgt
    15961 gttagtttct ggtgtctttt ctaaaattaa aaaactattc cctcctatag taacaggaac
    16021 ggttatcacc gttattggtt tgactttgat tcctgtagca atcgagaaaa tgggtggagg
    16081 taatgcaaca gcagctgatt ttggtgacaa aacaaatctg ctacttgcat ttgtgacgat
    16141 tctcctaatt gtaggcgtgc aaatgctggc aaaagggttc attcgttcaa tcgcggtatt
    16201 gattggttta gtcggaggaa gcatcttagc tgctttcatg ggaatgattg atctgagcgc
    16261 gattggagat gcgccgttgt tccatgttcc acaacccttt tattttggta aaccaacttt
    16321 cgacgtatgg tcgattttac taatgatcat catttccatg gtcagcatgg tagaatcaac
    16381 aggtgtatat tttgcacttg gtgacattac tgggaaaaat gttggagaag aagaattgaa
    16441 aaagggatat cgtgcagaag gacttgccgt tatcttaggc ggtattttca atacattccc
    16501 ttatactggc ttttctcaaa atgtcggatt ggttcaactt tcgggaatca aaacaagaaa
    16561 acccatctat ttctctgcat tcttcttgat tgttttagga ttattcccta aaatcggtgc
    16621 gttagcacaa atcatccctg agccggtact tggtggagga atgttagtca tgttcgggat
    16681 ggtggcagtc caaggaatcc gtatgctgtt ggaagttgat ttcagcaatg ataaaaatct
    16741 attgattgtt gctgtttctg ttggatttgg gctgggtttc aatgttacgc cgacattatt
    16801 cagtcaaatg ccgcaaacat tgcaaatgtt cactggaaat ggcatcgtaa tgagtagcat
    16861 tacggctatc atattgaatt tgatctttaa tggattgaaa aaagatgaag aaatattgga
    16921 agaagccttc aagaagtaat agagcaaaag aaaggttgtg aaatcagcgt tttgacctga
    16981 ttgttagaga aacaaacgga aaaatagttc ctcgtatttt gccgtttgtt tgagctaata
    17041 ccgagggtca gctgattgaa caccgtttat ccataaaaag gaatgtgacc taacttttgt
    17101 cacattcctt tttttcatct ttaataaaca cgaacaataa aatgtttttc tttgttaaca
    17161 ttcgaaattt ctttgacatc aaaaaaagtg attgttaaaa tatgaatgta gaaaaactgg
    17221 gaggaaaaac aatgacagca aaaatctctg tgattatggg gagtacttca gattgggaaa
    17281 caatgaaaga agcatgtcaa atattggatg aattcggtgt gtcttatgaa aaaaaggtcg
    17341 tttctgctca tcgaacccca gatttgatgt ttgaatatgc acaaaacgct cgaaaaaaag
    17401 gaatcaaaat cattattgca ggagcagggg gagccgcgca tctgccgggt atggtcgcat
    17461 caaaaacaac tttgccggtt atcggtgtac cggtccaatc acgaacatta aatggtttgg
    17521 attcgcttct ttcgattgtc caaatgcctg gaggagtacc tgtagctact acagcaatcg
    17581 gaaaagcggg agcaattaat gctggactac ttgcgataca gatgctttcc atgtatgatt
    17641 tagaattgga ggctaaattg gctgaaagac gttcaatgtt ggctaaaact gttatagaaa
    17701 gtagtgatca acttggttaa acctttgttg cctggttcaa cgatcgggat cgtcggtggc
    17761 ggtcaattag gtcgtatgat ggcgattagt gcaaaagaaa tgggctttaa agtaggtgtg
    17821 ctggatcctg tgagcgattg tccagccgca caagttgcag attggcacat tgaaagtgtc
    17881 tatgatgaca cctttgcgct gaaagagctt gccagaagaa cagatgtcat tacctatgaa
    17941 ttcgaaaacg tcagtgtaga ggcgttaaat gcgattttac ccatgtcatt tattcctcaa
    18001 gggacagact tgcttgcgat tacgcaagac cgcctgttag aaaaatcttt tttggaaacg
    18061 aataacatcg tgattgctcc ttatgcaacg atcgtcagtc cgacggatat ccaagatgcg
    18121 attgacggga tcggctatcc ttgtgttttg aaaaccactc gaggcggcta tgatggcaag
    18181 gggcagtatg ttttgaaaag tcgggcggat ctagctcctg ccatggacct tttaagagaa
    18241 ggaacctgtg ttttagaagc atggatcccg tttgaaaaag agatttcgat catggtggca
    18301 ggaaatggtc aaggagattt cacgactttt ccggtcgtgg aaaacatcca ccacaacaat
    18361 attcttcatg aaacgattgc tccagccgcg attgatcagg atgtgatcga ggaggctgag
    18421 cgtattgccc gtgtgattgc agaagcagtg agcctttcag gtgttttagg ggtagaaatg
    18481 tttttaacaa aaacaggcgg gctatatgtg aatgaattag ctcctcgtcc tcacaattct
    18541 ggacattatt ctattgaagc ttgttctatg agtcagttcg atgcccatat tcgaggtatt
    18601 tgtggttggc cattaggaga aaaagtacgt ttgataagca atgcagtaat ggtcaatgtt
    18661 ttgggagaac aactgctgga aagctatcgc ttgattgaga ataagccaga ctggcaattc
    18721 catttctatg gaaaaaaaca agcaaaaaaa ggtcgcaaga tgggacatat cactatcttg
    18781 acagaagaca tccagcaaac gctggaagaa atcaaacaaa cgaatatttg ggattagagg
    18841 agaaaaaaca tgttagaacg ttatacacgc cctgaaatgg ggaatatctg gacggaagag
    18901 aacaaatacc gtgcgtggct cgaagtcgag attttagcag atgaagcttg ggcagaactt
    18961 ggtgaaatcc cgcaagaaga tgtgaaaaaa attcgtgagc atgcatcatt tgatatcgat
    19021 cggatcttgg aaatcgaaaa attaacacgc catgatgtcg ttgcgtttac acgagcggtt
    19081 tctgaaacat tgggagaaga aagaaaatgg gtccattatg gtctaacaag tacggatgtc
    19141 gtagatactg cttatggcta tctcatcaag caagcaaatg atattttacg ggaagacttg
    19201 aaacgtttta cggaaatcat tggcgaaaaa gcaaaagaac acaaatatac agtgatgatg
    19261 ggtcggacac acggtgtcca tgctgaacca actacttttg gattgaaact tgccacatgg
    19321 tattcagaaa tgaaacggaa catcgaacga tttgaacatg cggcaaaagg agtagaagca
    19381 ggaaaaatct caggagcagt gggaacgttc gcaaatatcc cgccatttat tgaagagtat
    19441 gtatgtaaac aattggggat ccgtccacaa gaaatctcta ctcaagttct ccctcgggat
    19501 ctacatgctg aatatttctc agcgatggcg cttattgcta caagtattga acgttttgca
    19561 acagagatac gcggattgca aaaatccgaa acacgtgaag tagaagaatt ttttgcgaaa
    19621 ggtcaaaaag gttcttctgc tatgccgcac aaaaggaatc cgatcggttc agaaaatatg
    19681 gctggtctag ccagagtggt gcgcggacac atggtcactg cctatgaaaa tgtcacatta
    19741 tggcatgaaa gagatatttc tcattcttca gcagaacgaa tcatcttgcc tgataccacg
    19801 attttattga attacatgtt aaatcgtttt ggcaatatcg ttaaaaatct aacggtcttt
    19861 cctgaaaaca tgaagcggaa tatgtctgca acatttggtt tgatttatag ccagcgcgtt
    19921 atgctgaaat tgatcgataa gggaatgaca agagaagaag cttatgatct tgttcagcca
    19981 aaaactgcgt acgcttggga caaccaagtg cagttccgcc cgctgttaga agaagatcca
    20041 accatcacat cggtattatc taaagaagaa atagacgatg cctttgatta tcatcatcat
    20101 ttgaaaaacg ttgacgtgat attcaaacga gtaggtttgg aataaagaag agtgagttgc
    20161 aaaattcaat taaaatagaa tagcaaaaat aaccaaaaaa gaagcagatc cgttagcggt
    20221 tctgcttctt ttagtttatt atattttctt atcaataata ggaacctgat tttaaaagcg
    20281 gctacctctt taccgtgcct ctttacatac aaaaaataga ggaattaagc gtaaatggct
    20341 tggctattat aactaaaatg ctggttatgg agatgttgca gcaagttttc gagaaaagcg
    20401 tattccatct cttgtccatt gaataagatc tggatacgat aatgattttt gaaacgcttc
    20461 tgttcatgaa tctgactgat tgaaagttcg cctttgtgtt ctgtctgata aagaaaagtc
    20521 cttttacggc attcagcaca gcgcttggtt gctcggcgga tttttcggaa tgtataaaaa
    20581 ctagtatctt tattgcataa ataaacgtct ttgatcgaca gcaaatcatt atatagacag
    20641 gaacgttctt cagctgttaa ttctgataaa aaaagctgac cttgttttat tgcatcttcc
    20701 atcggcaagt gcctcctcga acccattatc caaattttta gttaattcta atataccatc
    20761 attaagtata aatgtaaacg cttgttgatg gtataagatt ttcgtttctg ataaaacacg
    20821 aacattaaaa gaatctaatg ccttaaatgt acatattttt gttgatttat aaaaagatga
    20881 ttgctaaaat ttaaagtgaa aaaacgaaca aaaaacgatg tatttgcttt taatgtaaat
    20941 aaaaaggaga gacgtacatg gagaagaaag aacttttgta tgaaggaaaa gccaaaaaaa
    21001 tatatcttac ttctgatgag aacttgattt ggatagaata tttagatcaa gcaactgcgt
    21061 tgaatggcgc gaggaaagac cagataagtg gaaaaggaga attgaataat caaatcacaa
    21121 gtgcaatttt ccgttatctg actcaaagag gaataaaaaa tcattttatt gaacaattgt
    21181 ctaaaaccga acaattagca aaaaaagtac aaattatccc tctggaagtg gttatccgaa
    21241 atattgcagc aggaagtttc tcaaaacgtc taggtgttcc cgaaggtaaa gaattcatca
    21301 aaccaatcat tgaattctac tataaggatg accagttgga tgatccattt atcaatgaag
    21361 agcatatcta tttcttacaa cttgcaaaac cggaagaaat cagtgagctg aaagcccaag
    21421 cgttggctat taatgaaatt ttgaccgaat tatttgctaa ggtcggtttg gttttagtag
    21481 atttcaagct agaatttggc cggtcgaatg gacaaatttt gcttgcggat gaagtaacac
    21541 cggatacttg ccgattatgg gatgaacgta cgaatgaaca tatggacaaa gacgtttacc
    21601 gcagagatct aggtgatttg atcccagtct atcaagaagt atataaccgt ctatcggaac
    21661 taactgaaga aaaggagcga gtatgatgta tttcgtaaaa gtatatgtaa cctataaaga
    21721 ttcagtgtta gatcctcaag gggaggcagt aaaaaaagca gtccatcgta tgggatatga
    21781 aacaatcgaa gatatccgaa taggaaaata ctttgagata caacttgctg atgcagaaga
    21841 accaatcgat acagtgattg aaacgatttg tgacaagctg cttgcaaatg taaatatgga
    21901 aacataccga tatgaaatca aaaaaatgga ggaagtataa catgaaattt gcggtcatcg
    21961 tttttccagg ttcaaattgt gacgcagata tgttatgggc agttcgcgag atcatgggcg
    22021 ctgatgccga atttgtacgg catgatgagt cctctttaga aggatttgat ggggttttac
    22081 tgccgggagg attctcctat ggtgactatt tgagatgcgg tgcgattgct cggttttcta
    22141 caatcatgga agaagtgatt cgttttgctg aagaagggaa accagtattc ggtacatgca
    22201 atggattcca gatattgaca gaagcaggac ttttgccagg tgtactgaga cgaaatgagt
    22261 ctttgcattt tatttgcaaa actgtaccat tgaaagtcaa ccccaagacg gcctttactt
    22321 cagagtatga agccgacgaa ataatctatc ttccagttgc tcatggggaa ggaaattatt
    22381 tctgtgacga agaaacactt caagaactaa aaaaaaataa tcagattgcc tttacttata
    22441 atgacaacat caacggcagc gtggaaaaca ttgcagggat tacgaacaaa gcagggaatg
    22501 tgttaggaat gatgcctcat ccagaacgtg cagtagaaga cttgctagga tcagaagatg
    22561 ggaaacggtt ctttgcttcc attttaaaaa acttcggaaa ggtgaccgta tagatgaaaa
    22621 cattgaaaga accgacagct gaagagatca agaaaaaaag aatctatgcc gaatggggat
    22681 tgactgatga agaatatcga atgatcgaag aagatatctt aggaagaatg cctaattata
    22741 cggaaactgg actgttttct gttatgtgga gcgaacattg ttcctataaa aattctaaac
    22801 cggttctacg caaatttcct actagtggac ctcatgtgtt gcaaggccct ggagaaggcg
    22861 caggcatcgt cgacatcggt gatggactag cagttgtctt caaagcagaa agtcacaatc
    22921 atccttcagc cgtggagcct tatgaaggag ctgcgacagg agttggcgga atcatcagag
    22981 atattttcag catgggggct cgtccaattg cgttgctaga ttcattgagg ttcggcgaat
    23041 tagataatcc aagaacgaaa tatttactag aagaagtagt tgcaggtatc ggcggatacg
    23101 gcaattgtat cggcataccg actgtaggcg gcgaaattgc atttgatcct tgttatgaag
    23161 gaaacccatt agtcaatgca atgtgcgtag gtttgattga acacaaacat atccagaaag
    23221 ggcaagccgc tggcgtcggt aattcgatca tgtatgtagg tgcaaaaaca ggtcgcgacg
    23281 gtatccacgg agcaactttt gcctcagaag aatttaatca agaagaagaa cagcaacgtt
    23341 ctgctgtaca agtaggcgat ccttttatgg aaaagctgct gcttgaagcg tgtttggaat
    23401 taatcttgca tcattcggat tctctaatag gtatacagga tatgggagca gcaggacttg
    23461 tttcatctag tgcggaaatg gcttcaaaag ctggctcagg acttatattg acattagatg
    23521 aagtcccaca aagagaaact gggatgacac cttatgaaat gatgttatcg gaatcacaag
    23581 aacggatgtt gatttgtgtt aaatcaggag aggaagaaaa aatccaagaa ctatttcaga
    23641 agtatgatct agatgcagtc actatcggta aagtaacaga cgacgggcaa tatcggctat
    23701 atcatcaagg agaagaagta gcaaatctgc cagtcgatgc attagctgaa gatgcaccag
    23761 tttatcataa agaaatgaaa gaaccagctc gaatcgtaga atttcagcag ttagctcctt
    23821 atcagccagt tatcgaagaa cctggacaag tattgcttga tctgctgcag caaccgacga
    23881 ttgcttcaaa acgttcaatc tacgaaactt atgattcaca agtacaaacc aatacagttg
    23941 tccgtccagg aagcgatgct gctgtgatga gggttagagg gacgaataaa gcgttagcta
    24001 tgaccacaga ctgcaacgcg cgctatctgt atttggatcc aaaaatcgga ggacaaatag
    24061 cagtggcaga agctgctaga aatattattg ctagcggtgg taagcccttg gcgatcaccg
    24121 actgcctgaa ctatggttct cctgataaac cagaagtttt ttgggaatta tcgacttcag
    24181 ctgacggaat tgctgctgcg tgtgaaacgt taggaactcc tgttatttca ggaaatgtct
    24241 ctttatataa tgaaacggat ggtcaagcaa tctatccaac accaatgatt ggtatggtcg
    24301 gattgattga ggatcataaa catatcacta ctcaggaatt caaaaaatca ggggatctaa
    24361 tctatatatt aggtaagacg tttgcagatt ttgatggcag tgaattacaa aagatgcagc
    24421 ttggaagaat cgaaggtgtg atccgaaact ttgatctatc cattgaaaag agaaaccaag
    24481 agttagtatt gacagccatc caaaacggat tgattgaaag tgctcatgac tgttcagaag
    24541 gtggcttggc aattgcactg gcagaatcag ctttcaaaca tcagctaggg atatctgtac
    24601 agtttgaatt gtcctctgct caattatttg cagaaactca atcaagattt gtgttgacag
    24661 tcgcaccgga aaataagaca cgattcgaag aaatgatggg agatgctgct gttttagcag
    24721 gtaaggtgac ggatgaagcg ataatcgaaa tttcagcaac tgacggtcag atcaaaatag
    24781 aaactgcagt agcaagaaag tgctgggagg atgcaatcgt atgtcttatg aagtgaagag
    24841 tttaaatgaa gaatgcggga tcttcggtgt ttggggtcat ccagacgccg ctagtgtggc
    24901 atacttcggg ctccatagtc ttcagcatcg aggacaagaa ggtgcaggta tcgtgtccaa
    24961 tgatcgagga aagcttaagg ggcatcgaga tttggggctg ctttctgagg tattcaaaga
    25021 tcaaagaaaa ttgcagcagt taacaggaga agctgcaatc ggacatgtac gttatgcaac
    25081 agcaggcaat ggaagtgtgg acaatattca gccttttcta ttcaaatttc atgatcagtc
    25141 gatcggctta gcgcataatg gtaacttgat caatgctcgt tcccttagaa aagagttaga
    25201 ggaaaatggg gcgatcttcc attctaactc cgatacggag atattgatgc atttgatccg
    25261 acgaagtgaa gaaacagatt ttttaaataa attaaaaagt gctttgaatg aagtcaaagg
    25321 tggctttgcc tatttattaa tgacagaaac ggcaatgatt gccgctttgg acccaaatgg
    25381 ttttcgtcca ttagcaatcg gacaaatgaa aaacggagca tacgtgattg cctcggaaac
    25441 ttgtgcatta gaagttattg gggcacgttt tataagagat gtccagcctg gtgagatagt
    25501 gatcatcaat gacgaaggaa tccagattga ttgctttacg aaggacacgc agctttctat
    25561 ttgttcgatg gaatatatct attttgccag accagattca aacatcgcag ggatcaatgt
    25621 gcatactgca cggaaaaata tgggacggaa tttagcgaaa gaatcgccag taaaagcaga
    25681 tatggtcgtt ggcgtaccta attcttcttt gtcagccgca agcggttatg cggaagctag
    25741 tggaatccct tatgaaatcg gattagtaaa aaatcaatac attgcccgaa catttatcca
    25801 gccaacgcct gagctaagag agcaaggtgt tcgaatgaaa ttatctgctg tacgaggagt
    25861 agtggaaggt aaaaaggtga ttatggtcga tgattcaatt gttcggggta cgacaagcag
    25921 acggatcgtc catcttttga aagaagcagg ggcaaaagaa gtccatgtga ggattgcttc
    25981 tcctcctttg aaatatccat gtttttacgg gattgatatt caaacgcgta aagaactcat
    26041 tgcggcaaat cacacgattg atgaaatcaa agaatgtatc ggtgcagatt ctttaggttt
    26101 tttaagtgaa gagggtctga tcgaatcgat cggtctggaa aaagatgctc cgtattcagg
    26161 attgtgtatg gcttatttca acggagatta tccgacgccg ttatatgact atgaagaaaa
    26221 atatcaggaa tcactaaaag aaaaagtatc gttcttttaa aagcagacca agaagggagt
    26281 atatgaattc aatggcaaat gcatacgcaa aagctggcgt agatgtcgaa gctggatatg
    26341 aagtagtaga acggatcaag aagcatgtca aaaaaacgga acgtttaggt atgatgggcg
    26401 ctataggagg attcggcggt tgttttgatt tatcggtctt tcaattaaaa gagccagtcc
    26461 ttgtctcggg gaccgatggc gtggggacga aattagcagt ggcaatcgaa gcagacaaac
    26521 acgatacaat cgggatcgac tgcgtagcga tgtgtgtcaa tgatatctta gcacaaggcg
    26581 cagagccgtt atatttctta gactacttag caacggggaa aaatgatccg ttacgtttgg
    26641 aaaatgtagt aaaaggtgta gcagatggat gtgttcaagg aaatatggcg ctgattggcg
    26701 gagaaacagc tgaaatgcct ggtatgtact caggtaatga ttatgatcta gccggatttg
    26761 ctgtagggat agctgaaaaa tctcaattat tgacaggaga aacgatcaac ccaggagatg
    26821 tgttgcttgg aatagcatct agcgggattc attcaaatgg gtactctctt gttcgcaaaa
    26881 ttttctttga gcagcagcag ttttctttat catcaaaact gccagaattg aaaaaaacac
    26941 ttggcgaaga attgcttgag ccaacgaaaa tctatgtttc ttccttgctt ccgctgatca
    27001 aagaaaagaa aataaaagga gctgcacata tcactggtgg agggtttgta gaaaatatcc
    27061 cgcgaatgtt accagaatct atggctgcac aaatcaaact tggctcatgg ccgattcttc
    27121 caatattcgg ctgtttacaa aaatatggtg aaattcctga gatggagatg tatgaaatct
    27181 tcaatatggg catcggtatg gtcctggcag tcgaagaatc gaaagtaaaa gaaatagtac
    27241 acgaaattga gcaagctggc gaaaaagttt atgaaatcgg gaaagtgatc gccaaagaaa
    27301 aacatcgtgt ggtgtttata ggagaagaac gatgagaatc gctgtttttg cttctggaaa
    27361 cggaagcaac tttcaagcgc tagccgatta tctatcgaaa aaaggattgg aatcttcgat
    27421 tgactggttg ttttgtgatc agcctgaagc atatgttttg aaacgtgcaa ctgcgttgag
    27481 tgtaccggca gattgttttt cacctaaaga atttgattca aaaaaagagt atgaagaagc
    27541 aattttgcat aaattgaaag aaaaaaagat cgatttgatc gtattagctg gatatatgcg
    27601 aatcattgga ccggtattgc tgaaaaatta tgataaaaga atcatcaata tccatccgtc
    27661 tcttttacct gccttccctg gtttgcatgg catacgtgat gcctttgaag ctggagtaaa
    27721 agaaacgggt gtcacaattc attatatcga ccaaggtgta gatactggac caatcatccg
    27781 gcaggaaaaa gtgagaattg aacaggagga cacgttcgat tctttagaag aaaaaatcca
    27841 tagagtagag catcgaatct atccagaagt gattagtgaa attattgaaa atggaggcta
    27901 tctgaaatga caagagcatt gatcagtgta tcagataaaa ctgggattgt agcattcgcc
    27961 caaggtttga ttgaacaagg agtagaaatc atttcaacag gaggaacaaa gcatgcgttg
    28021 gataaagctg ggatcaaaac gctttcgatc gaagaagtga cagggtttcc tgaaatgatg
    28081 gatggccgtg tcaaaacact tcatcctaaa atccatggtg gtcttttagg aagacgcgat
    28141 ctccctagcc atatggaggc aatggaaacg gaaaatatcc aaccaatcga ctttgtttgt
    28201 gtcaatttat atcctttcaa agaaacgatt atgaaaccag atgtcgaaat agcagaagct
    28261 attgaaaaca ttgatatcgg tggtcctagt atgctccgta gtgcagcaaa aaaccatgaa
    28321 tttgtcacct ctgttgttga tccatctgac tatgaagaag tactttcaga attacgtcag
    28381 tatgggcata cttcactttc tttcagacaa aaactagcgg caaaagtctt tcgtcatact
    28441 gcggcatatg atgcactgat cgctcaatac ttaacggatt gggtggaaga aaaagaaccg
    28501 gaaaaattga cacttacgta tgaacgaaaa caaacgcttc gttacggtga aaatagccat
    28561 caagccgctg ccttttatca gtccgctctt ctggaaaaat attcgatcgc agcagcaaaa
    28621 caactccatg ggaaagaact ttcttataat aatatccgag atgcagatgc tgcgctacgg
    28681 atcatgaagg aatatgacga gccaacggta gtcgccttga aacacatgaa tccttgtggt
    28741 attgggacag catctacgat tttagaagcg tgggaagcag cgtatgaagc agatcctgtt
    28801 tcaatctttg gtgggatcat cgttttgaat cgagaagtag atctgccaac agcagaacag
    28861 atgcataagc tgtttctaga aatcatcatt gccccttctt atacacaaga agctttggaa
    28921 ttactgcaaa aaaagaaaaa catccgatta ctgacagtag actttgcgca ggaagaaaat
    28981 caaaatcgag aagaaacggt ctcagtctta ggtggacttc ttgtacaaga tcaagatctg
    29041 gcaatagaaa atgaagaaga gtggaaagtc gtgacaaaac gtaagccgac tccagcagaa
    29101 gcaaaagcat tagcgtttgc ttggagagct gtcaaacatg tgaaatctaa tgcgatcgta
    29161 ctggcaaacg aacgtcagac agtaggaatc ggagccgggc agatgaatcg agtcggttca
    29221 gtaaaaattg cgattgaaca agcgcaagag aaaatggaag gtgcagtttt agctagcgat
    29281 gcttattttc caatggatga ttcggttgaa tatgcagcca aacatggtat aaaagcaatc
    29341 attcagccag gaggaagcat caaagaccaa gattctattg atatggcgaa taaatacgat
    29401 ctagcaatgg tctttactga tgttcggcat tttagacatt agagaggagt cggataaatg
    29461 aacattttgg tgatcggtgc aggtggtcgt gaacatgcaa taagtaaaaa acttttagcc
    29521 agtccgaagg tggatacggt gtattgtgcg ccaggaaatc cggggatgac aaaagatggt
    29581 atcaattgtg tcgctatcag tgagcgagat cattcagcgt taatccattt tgttaagaaa
    29641 gaggctattg attggagtat cgttggtcct gaagttcctt tgttaaatgg gatcgtcgat
    29701 gattttatgg cggaaggatt gaaaatcttc ggtccgacaa aagcagcagc tctgatcgaa
    29761 ggttcgaaaa gttttgctaa acaattaatg gatacttatc aaatcccgac agctaaatcc
    29821 agaagttttg aatgttacga aaaagcagca gcgtatatca aagaaaatgg tgcgccaatc
    29881 gtagtgaaag cggacggctt agcagctggg aaaggtgtag tcgtggctac cacagtgaag
    29941 gaagcgctgg atgcggcaga acaaatgttg aaccagcatc gatttgggga tagtagcaaa
    30001 aaaatcgtcg tagaagagtt tttggcagga gaagaattct cccttttggc gtttgtccgt
    30061 aataccaagg tgtatccgat ggtcatttcg caagatcata agcgggcatt tgataacgat
    30121 aaagggccaa atacaggcgg aatgggtgct tattcaccag taccgcaaat ttcaaaaaag
    30181 atgattgaaa aagctgtgga agaagtttta aaaccagcgg ctttgggaat gaaagaacta
    30241 ggacgctctt tcacgggtgt tttatatgcg ggtttgattg ctacgaagga aggaccaaaa
    30301 gtgatcgaat tcaatgcgcg tttcggtgat cctgaaacgc aagtcgttct ttctcggttg
    30361 aagagtgact ttgcgcaagt aatcgatgat ttattagaga atcgtacagt agagttaaag
    30421 tggcagcaag aaggatacaa tgtaggcgtt gtggttgcag ctgccggtta ccccgaagaa
    30481 tatgagacag gaatggttct accagatttt ctggaagaag agctttctgt ttactatgct
    30541 ggagtttcct caaataacgg caaactagtt ggagcaggag gacgtcttta tttagtagaa
    30601 gcatacggtg agacgatcaa agaagcacag aaaaaaattt acaccgcgtt aatggataaa
    30661 acgacagatg gaacatttta tcgtacagat attggagaaa aagctgccaa tagcgaactg
    30721 atcgtgaagt aaatcagctg cttccattta taaaaaatac aacaaacata atcggaagcg
    30781 ccgattttag tgaagccttt tcataaagag tgtggtacac ttttgttgtg tatataaata
    30841 aggaggagtt cagatgaaaa aaattcaatt aggtacttct gatcagaaag cagctgcagt
    30901 gatcttaggc tgtatgcgta tcaacaatgc ggaagatcca gtaaaagtga ttgagacagc
    30961 ttatgaaaac ggcattgatt tttttgacca tgcagatatt tatggaaacg gtgaatgtga
    31021 agaaatcttt gcagatgctc tagcaaaaac atcaatcaaa cgagaagatc tttttattca
    31081 aacaaaatgt ggaatcgttc caggtgtgat gtttgatttt tctaaaaaac atatcattga
    31141 atctgtagaa aaaagtttga cccgtctgaa aatggattat gtggatgctt tacttcttca
    31201 tcgtccagat acattggtag aaccagagga agtagctgaa gcgtttgatg aattggaacg
    31261 gtcaggaaaa gttcgttatt ttggcgtcag caatcaaaaa ccaatgcaaa tcgaattgct
    31321 gaaaaaatat gttcgtcaac cattagtagc taatcagctg caatttggct tgaaacatac
    31381 tggaatgatc gatcaaggga ttcatgtcaa tatgacagat gaagcaagta tcgatcatga
    31441 cggtagtgta cttgattatt cccgattgca tgatatgacg atccaagcat ggtcaccata
    31501 ccagtatgga ttttttgaag gtgtatttat cgggaacgaa aaattccctg aattaaacca
    31561 aacattagaa gaactagcag ctaaatatca aacgacacca actggcttgg cttctgcttg
    31621 gattttacgc catccagcca atatgcaagt gatcgccggt acgatgaacg tagggcgaat
    31681 caaggaaatc gctcaagctt cagaaattaa tatgtctcgg gaagattggt acgaattgta
    31741 ccgtgctgct ggaaacattt taccttaaag attgtcataa gcaggtcaaa agggctataa
    31801 attaaaaaga cagaataact tgaaagctgt tttcagcttc gagatattct gtcttttttt
    31861 attgcaaatg gaaagagtat cattgtacgg atttcgttta ttagttattt ttgtttttaa
    31921 ggaaaagctg tgtccgatat tcttttggag aaatcccttg ttttttctta aataaataac
    31981 tgaaatagtt agggtcatta taaccaattt caaaagcaat atctgaaata cgatagtctg
    32041 attgctgcaa taattttttt gctaattcga ttctttgttc tgtcagataa tcgatgaatg
    32101 tttggttaaa cgattgagaa aaaatcgtac tgaaatgaga gggacttaat gcaacttgtt
    32161 gagcgaccca agttaaggac atatctggat cggtaaaatt ttctttgata aaatccaatg
    32221 catggttcaa aattggctga tatttgttcc ttgaaggctg tgagggcaga gcagttaaga
    32281 aagaaatgaa catataaact gtcgaacggt aaagcgagtc gtcggcgctg attgctaagc
    32341 gttgactggt ggatgtgcaa tgaaccattt cttgcgggat tttcttttct ttcaggcaga
    32401 catgctggag tagcttagtc aagacaaata aacgatataa tcgtgttcga gttggtgtgc
    32461 cttgtggttt cgtcaagtgt tcgatgtact tagatacttc gtttttcgat aatgaagaca
    32521 aatcaagtga gaggtcaaag taaaagacag aatcaactgg aagtttcgct tcttgaatca
    32581 aatcctcata gctgataatt tgttctttaa gggaactggc ggtttcaagt atcatctgcg
    32641 tgatctcaaa agatgcaggt aattcgctga ttctttctgc gacaggacca atggcgacag
    32701 aaaaattatt tttttgtccc ttttctaatt catggaccaa cgtttgggct acctgataac
    32761 tggtctcgat cacattttct tcaagagaat caactattaa aaatttgatg aatcgagagg
    32821 aaacagcaga gaagatcact tgttgattct ccctaaataa aaacttcaaa taatcactga
    32881 gatgagcgta atcttcaaag tctgtactga cttggtttgc tgctaataat acggtcattt
    32941 ttttccctgc taaagagcga ttgaaagatt cagcaagttt caatgtttca ctgatcgtaa
    33001 gtgagccttt gaacaaaccg ttcaagaaat gatttttttt caattcgaaa acaagatcag
    33061 aagaagtgtg cgtttctttt tgctgcaaat ctaaagcagt tactgctttt tctattgctt
    33121 gtttcaattc gctactttta atcggtttaa gcagataatc atctgcctgt acctggattg
    33181 ctcctttcat ataatcgaaa tcgtcgaatc cactaataaa tatgagacga gtccaaggaa
    33241 gtagtttcct tgcttctttt gaaaaagtca acccgtccat gaaaggcatt tggatatcag
    33301 tcagaatgat gtcgggctgt aagtcgataa ttgaagacag tgccatctca ccatctccag
    33361 cttcgccgac ataagtgatt ggataataag aaccaaggac agttatttgt tcccgcaagt
    33421 tattgcgaat gaggtattcg tcttcgatga taaaaacttt atacatgttt gtttcctcca
    33481 attgttttgg gagatagagg gatcatcagt gtgacagttg ttccttctcg gtatttgctg
    33541 tcaatcgtga taggtttttg atcatttccg taataaagaa ggagacgctt gtttacgttg
    33601 taaagtccgt agccgatact gaaatctgaa tcgatccctt tggctaactc tttacgtatt
    33661 tctgccagtt tttctgcagg gatgccaatc ccgttgtcta taactttgaa ttggatcatc
    33721 tcatcaattt tgcatgcggt aatacgaata aatcctttgt gacggacgta cttcgttcca
    33781 tggtatacag cattttcaat caatggctgc aagatcattt ttaaaataga aacaggaaaa
    33841 agtgtttctg gtatatcgat cttatagtca agcatatggc cgtatcttat ttttaagatg
    33901 actagataat cattgatatg gctaatttct ttttcaagag gaatccagtc ttgtcctttg
    33961 cttaaagacg tacgaaaaaa gccggaaagt gcatgggtca tagtcagagc ttcttcaaag
    34021 cagtcttgtt cgatcaaaga aataatggca tccaaactgt tataaataaa atggggagta
    34081 atctgagctt gcaatacacg aacttcactt tgtgctaaat ggtattgttt caaagcgttc
    34141 tcccgtaaag actggtttag atcttccgcc attttattca tgctagcggt aagtgtttgt
    34201 aattccagtg tttccggcag ggttaaacga aagccaaggt tccctgaaga aagttgttct
    34261 gacatctcga tcagctgatt gatcggttct tggacctttc tctctaaaaa gcgacgattc
    34321 tttttcgcat agaaaaaaat ggtactaaga actccaagtt ccactattgt taatagaatg
    34381 agcgagcgga caagtgtcgt attttttttg cttgctaagt cgatctccac acgaacaaaa
    34441 tcttgtaaga tatctgacag cagttgagtg acagaatcta cctgattgat gatctcttca
    34501 ttggtatcca taaaatcttt tgccaagata ttattgatca gctcgttttg ataatcttcc
    34561 agtgtatcga tcgtacgtag cgatacatct aacgtactaa tttctttaag tgtatttgta
    34621 ttatttttga tctctcgaat atcattccta agttcttcta ggatattttc ctcattgaac
    34681 tcatctacgg ttacttggcc agaaatcaag tcccacatac tttccaaaac tttttgatca
    34741 accttcaaag caattgcatc tgctttttgg atatttgcga tcgtctgttg ataaatcaaa
    34801 agctgttgcg aatataacag tatgctcagt agcaaaggga taacagcaaa tagaagcatg
    34861 gctttgtttg cttgggcaaa aatctgtttt atgcttaaat tttttgtcat gatacttctc
    34921 cagtcaataa tttctggttg ggatgttagc tatattctgc tgggaaaaga cagtctcgga
    34981 aataaaaata tctttaggta atgtttttcc tgtttgaata tttccaaaat aacgagtaac
    35041 ggtattggct acataccagc cgacatcagg tttacactcg actacacaat tgacgattcc
    35101 tgagcgtagc ttctcgatca tatcttcttg tccatcaata gttacaatga tcaaatcttc
    35161 tccaggtttg attgatgttt cttttatcgc agctaaagca ccgtgagtca tttcatcact
    35221 atggctgtat aatacatcga tttcggataa atcatttgtt tctaaatatc gtttcatctg
    35281 gtcttttcct ttcatccgaa taaaatcacc gacaattcgg tgaacaatag tcatcggagg
    35341 tttatggtcg atggttccat ctttttttag ggttggttta cgattgattg attccaaaaa
    35401 tccttcggag cgtagcttgg tcggtgtgga gttctctgaa ccagcaagtt ctaggattct
    35461 gatttcctgt ttctttgaat ttgtgaagtg attgctgaca aataaacccg cccgatttcc
    35521 ttctgctttg aaactggggc caatgtgggt gacgaaaagc tcgggatcat tcgtttgaat
    35581 atgtctgtcg acaagtatga cagggatccc tgctcgcttt gcttctttta atacagagtc
    35641 ccacccggat tcttgcaaag gagtaaaaat gatcatatcc actttatagg caatgaacgt
    35701 ccgtatatct tggatttgcc gttcctgatt catgtagcca tttcggtaga gaacttggta
    35761 atcttctttt tcaagcgcag tgataattga ttcagtatgc tttttccgcc agctactctc
    35821 cgttcctgat tgcgaaaaac caacgactag tgggtcaggt gcttcctttg tattaggtgt
    35881 acaccccatc atttggtaag cgattacaac aagggctaaa aaaaataaaa cacgtttcac
    35941 tttaaatcac tccatctgga tttgttagta ctatcttatc agataaaagg gaaaatcaaa
    36001 gtcaaaagga atcaatcgaa aaaaatccaa gaaacttaaa aaaatccaag acaaaaaaag
    36061 tacacctaaa aaatttaaag taaatcccat aaaaaaatca atgttaacgc tttctttatt
    36121 agctatactc ggtgtatcaa ttgaaatggg ggataaaaaa tgaaaaacaa atttagaaag
    36181 attgttggga ctatcgctat tttgaccgca agcattctat taagtgcgtg tggcaattca
    36241 ggatctgcag atgatagtac ggggtatgta ggaatcgcta tgccgaccaa atcagcagaa
    36301 agatggatcg ctgatgggaa caacatggtc tcggaattag aaaaactggg ttataaaaca
    36361 gatcttcaat atggagaaga taaagtagaa aatcaagtag cacaaataga aaatatgatt
    36421 acaaaagggg tagatacact tgtgattgct tctatcgatg gttcggcttt aactgatgtg
    36481 ttagctaaag caaaagaagc agatatcaag gtgatcgcct atgatcgttt actgatgaat
    36541 tcagaaaatg tcgattatta cgcgactttt gataattttg gagtaggtgt atcgcaagca
    36601 gcttatattg aagagcactt aggattgaaa gaaggaaaag gaccattcac gattgaatta
    36661 tttggtggct cgcctgacga caacaacgca ctaatcaatt acaacggagt gatgtctgtt
    36721 ttacaaccat atatggacaa cggtcagttg gtcgtgccat ctggacagac gagtttcagt
    36781 caaatcgcta cgcttcgctg ggatggttct acggcgcaag caaggatgga taatctgtta
    36841 agcgcaaatt atactgacca aacattggat gctgttttgt cgccatatga tccaataagt
    36901 ttaggaatca tttcgtcatt aaaaggcgtg gggtatggga gtgaaagtaa acctttacct
    36961 gtgattactg gacaagatgc gacagttgct ggcgttaaat cgatcattgc cggggaacaa
    37021 acacaaacga tctttaaaga cacacgtatc ttagcaaaaa atacgattga aatgatcaaa
    37081 gcaatcagtg atggagaaga ggttccggtc aatgatactg aaacttatga taatggggtg
    37141 aaaactgtcc ctacgtattt agcaaataca gtatctgtcg ataaagataa ttatcaagca
    37201 gagctgatcg acaccgatta ttataaagaa tccgatctga aaaattaaga ggaaccaaac
    37261 aaggaactaa agagatctaa gtcagagaga ggataagaat tatggcggat tgtattttaa
    37321 aaatggcaga tatcaccaaa aaattttctg gaatccgagc gttagattgc gtgaatctga
    37381 tgattgaacg tggggaaatc catgcattgt gcggagaaaa cggagcggga aaatcaacat
    37441 tgatgaatgt cttgagcggc ttgtatccat atggctccta tgacggggaa atcttttatg
    37501 atggaaagct gtgtcaattt aaagatttga aagatagtga agaaaaaggc atcgtcatca
    37561 ttcatcaaga attagcacta agtccttatc tttcaataaa agagaatatt tttctcggaa
    37621 atgaacaagc agaaagaggc gtgattgatt gggaactgac agaaaagaaa acatatgaac
    37681 tgctgaataa agtcggtttg tctatcgacc cgaatcttct agtatcccag attggcgtag
    37741 ggcaacagca actagttgaa atagctaaag cattatcaaa atccgtccgt ctgttgattt
    37801 tggatgagcc taccgctgca ttgaatgaag aagaaagcgc gaatttgctg agattgatcc
    37861 gagagttgaa agagcaaggt gtaacctcca tcattatttc acataagctg aatgaaatcg
    37921 tggatatcgc agatcgaatc accattttac gggatggaca gacgattgag acactagaaa
    37981 atgaaatgat cagcgaagag catattattc gcgggatggt gggcagagat ttagttgatc
    38041 gttatcctga aagaaaaccg gatattggag aagtttattt taaagtagaa gactggacgg
    38101 tctatcaccc tcttgatcac gagcgaatca taaacgacca catcaacttg catttgaaaa
    38161 aaggagagat tgtaggaata gctggactga tgggagcggg cagaactgaa ttcgctatga
    38221 gtctatttgg acattcttat ggaagcaaga tatccggacg tatattaaag gaaggggcag
    38281 agatatcagt taagaccgtt ccttcagcca ttgcaaacgg actcgcttat gtctcggagg
    38341 accgaaaatc tttaggactg aatctgctga tggatattcg ggaaaacgtg acgctgagca
    38401 gtttggataa aataagtcaa aaaggagtat tagataaaga aaaagaagta actgaggcag
    38461 aaaatttccg gaaaaagatg cgtataaaaa caaactccat ccatcaaatt gtcagtagtc
    38521 ttagtggtgg gaatcaacaa aaagtcgttt tatccaagtg gctgatgaca caacccgata
    38581 ttcttttttt ggatgagcca acccgtggga tagatgttgg ggcaaaatat gagatctaca
    38641 cgattatcga agaaatggca gcaaatggca aagctgtctg tcttatctct tctgaacttc
    38701 ctgaaatatt agggatgtgt gatcgaatct atacgatgaa cgaaggacgt attacaggag
    38761 aagtctcgcg agaagatgca gatcaggaag tcttgatgaa attaatgacg aaagaagagg
    38821 tgggttaagt tggaacaggc aaaaaatctt acggataaaa aagagaaaaa actaaatttg
    38881 aaaagtagag cagctgatct ttttggtcgc tacagcatgg tgatcatttt agcgggacta
    38941 cttattgcgt ttcaattgat gacggatggt attttctggc gtccattgaa tataactaac
    39001 cttgttctgc aaaatagcca tatattagta ctagctgcag ggatgttatt agttgtgctc
    39061 ttaggtcatg tggatttgtc ggtagggtca gtgatggcgt ttgttggtgc aattgcgggc
    39121 atgatgatgg tcaatcgcca aatgaacccg tggatgacag ttatcgcatg tcttgcagta
    39181 ggcgggctga tcggcgcatg gcaaggtttt tgggtagctt atgcagggat accggcgttt
    39241 atcgtgactt tagcaggatt gctgatgttt cgtggactga cacaagttgt tctaggaggc
    39301 caatctctag caccttttcc agctgttttc caaaaaatgt ctacgggtta tttaccagac
    39361 ttaagcgaag gaagcagcct tcacttcttg acattgattt taggaatgat catcacgatc
    39421 gtgttagttg ttggtcaatg gcgaacaaga aatcgtcgga aaaatcattt atttgaagtg
    39481 gaatctccaa cagctttctt ggtaaaggcg gtatttacag cagtcgttgt actaggaata
    39541 acttatatct ttgcctctta tcaagggtat ccgattattt tgatcattct tggtttgatc
    39601 gttgcagcat atgggttttt gacaaataaa acagtagcag gtcgacagat ttatgcgacg
    39661 ggcggaaatc gaaaagcggc tgaactatca gggatcaaaa caaagcgaat cacgttctgg
    39721 gtattcgtca acatgggagt catggcagct ttagctggat tagttttagc tgctcgattg
    39781 aatgcggcaa cacctcaagc aggtacatcg atggaactgg atgcaatggc ggcagtatat
    39841 tttggtggtg cttcgacttc cggcggcatt ggaacaatca tgggtgcgat tgtcggtggc
    39901 ttggtcatgg gtgtattgaa caatggcatg tcgattttag gcgtcggggt agactggcag
    39961 caggcaatca aaggattgat cttgctttta gccgttgtgc tagacttgta taataagaaa
    40021 aaacgaacaa cgtaggagaa cgccggttat tcagaaaaaa gactgtgaca agacatttgt
    40081 cacagtcttt ttttcgctaa aagcgaacat tcattgtttc gtttgtatag actaaaaata
    40141 atatatgttt ttttaccatt cgtataaatt ctctttttgc tcttgatttt tttgcttaat
    40201 tgagttaaat tacatagggc gctaaaaaaa gccttgatta aattaataat gttcgtgttt
    40261 tgctggatgt gaacatcagg aggataatgt aatgaaagta tttgattacg aagatattca
    40321 attaattcct aataaatgta tcgtaaatag ccgttctgaa tgtgatacaa cggtcacatt
    40381 aggtaaacat acattcaaaa tgccagtagt gcctgccaac atgcagacga ttattgatga
    40441 aacaatcgca gaattcttag cggaaaatgg ttatttctat atcatgcatc ggtttgatga
    40501 agcagctcga attccgttta tcaaaaaaat gaaaaagcgt ggattgatta cctcgatcag
    40561 tgttggcgtc aagaaagagg aatattcctt tatagagaag ttggcagaag aatcgctaaa
    40621 tcctgattat atcactatcg acatcgcgca tggacatgct aactcagtta ttgatatgat
    40681 tcagcatatt aaaaaatatt tgcctgaaac ttttgtgatt gccggtaatg ttggtacacc
    40741 ggaagccgtc cgtgaattgg aaaatgcggg cgcagatgca acaaaagtcg ggattggtcc
    40801 aggaaaagta tgtattacaa aaatcaagac aggctttggg actggaggct ggcaactggc
    40861 agctcttaga tggtgtgcga aagcagctcg caagccgatc atcgctgacg gtgggatcag
    40921 aacacatggc gatattgcaa aatctgtccg ttttggtgcg acaatggtca tgattggttc
    40981 attgtttgcc ggtcacgagg aatctcctgg cgaaacaaaa gtcgaaaatg gaatcgtgta
    41041 taaagaatac tttggttctg cttcagaatt ccaaaaaggc gaaaaacgaa atgttgaagg
    41101 aaagaaaatc tggatccaac acaaaggatc tttgaaagat acactagtgg aaatgcaaca
    41161 agaccttcaa tcatctattt cttatgctgg aggaagagat ctggaagcca ttcgaaaagt
    41221 tgactatgtc atcgtgaaaa attcgatttt taatggagac acaatctaaa aaataaataa
    41281 aatattagga tgttgtgaaa aagttgtcct gcattaagta ataagaacag ccaaacaaaa
    41341 atagttcttt atatgttttg tttggctgga attattatcg cagatacaag cttttgaaca
    41401 tcattattca gaataaggga ctgtgataag atttttgtca cagtccctta tgttttgcaa
    41461 aaaaagtttt tcttttttct cgtaaattcg gtaaaatcaa aagaaaaaat agacaataga
    41521 aaagagaagc ttatgtggga aaaagtagag aaaaaacaag gaccgattta tcgtcaaatt
    41581 atggaacaga ttatgagaga aatcgaaaac ggaaggatca atcctggaag tcaacttgaa
    41641 tcagaacgaa aactagggca actttttggt gtgaatcgtt ccaccgttgt ccatgcattg
    41701 gaagagctta gggagcttgg ggtactaacg agcaaacgtg gcagcggatg gtttgttaat
    41761 cagactgaat gggggaaatt cgctgttcca agaatcgatt ggcggcaaat gatctctcct
    41821 cgttatgagc agacggactg gtatgagcaa aaaataagag aagcaaagca gatccaaaaa
    41881 acatcgtttc ttgatttata cgctagcgag atgccagatg agcttttgcc gaattttgag
    41941 tttccttctt attctcttga agaaatctta gcggaagaaa aagaaatgga tcttttaggc
    42001 tatactccgt tacgtaacaa aatcaaacgg tatttggaaa gtaaatttga attggcattt
    42061 tcccccaacc agcttcttgt gactggtggc ggtcagcaag caatcttttt gattttacaa
    42121 acgattcttt cagctggaga ggggatagct gtagagtcac cttctttttt ttaccgatta
    42181 cctcttttca aagcttcagg aacgcgttta tttgggatac cgatggatga agaagggatc
    42241 gacttagaaa aactgaaaga atcgatccat aaaaataaat tgaaagcagt gcttatcaac
    42301 ccgaattttc aaaaccctac aggaaaagtg atgagtcaaa aacgacgaga aaagctagta
    42361 gaattgtgtc gacagtatcg gcttccaatt attgaagacg atgtatttac ggatctttca
    42421 tttctaaacc aagaagaagt ccttcctata cgaacgattg atccggataa tgtgttatac
    42481 gtcggttcgt tatctagggt tttaggaaaa acaacgaaaa tcggttgggt gataggacct
    42541 gagactttta tctcgcaact agctcaagcg caagaaatga tggagttttc gatgaatatc
    42601 cttacacaaa tcacagtggc taatgtattt aatgaagcaa ttgatttgaa aatgcaagat
    42661 gtaagaaaaa agttgaaaga aaaaagccga ctgataagcg cttggggaga ttcacaatcg
    42721 tttttcaagc ttcattcaat tttaggtggt tactatgcct ggattacttg ggatgggaaa
    42781 aaactagacc ccgaactagc agaaaaagta gtagatatgg gtcttggaat tgcaccaagt
    42841 ctactttttg ggcaagatat aaatggaatc agaatcaatt acagtaggct ggatgaaaac
    42901 caaataccgt tgttttattc aaagatgaaa attctagaaa agtggttaac agaataattt
    42961 cacaaagtaa atagattaaa gaatataaca gcttggattt aaatttcaag ctgtttcttt
    43021 tttttcggaa aaagaatacg atataaagaa actaagaaaa aaccattatt tgtacgtccg
    43081 ttcacgatgt gtaaataaaa taaatgtttg caacaaaact gtataacacc aacgttttta
    43141 aatagaaaca gatggttgta agtttgatta gaaaatagaa aaaatgcaga tgaaaaataa
    43201 aacgctttcc ttattgtgat tttattcact aatgctgtta atatgcggat tggcataatt
    43261 tcctttcatc gtgaaaattt gtgcttttag cttgtatttc tcataaatcc atgataattt
    43321 attaatgaag acggttaaat agtgatacag ttttattcac tgtttcaatc gcagaatgaa
    43381 gagattttct tatagatgag agaggaatgt gtgacaaatg gcaaacaaaa aagcaccgat
    43441 cgacttccaa gcactgctag atgaagtcaa tgcagatttc ccagtatatc aagcacttga
    43501 tcaagatgga aacgtagtta acaaagattt agtacctgat ttatccgatg atgaactcgt
    43561 tgaattaatg actcatatgg tatggtctcg tgtattagac caacgttcaa cagcgctgaa
    43621 ccgccaagga cgcctaggct tttttgcacc aactgctgga caagaggcaa gtcagctggc
    43681 tagtgcttac gcatttgata aagaagatgt tcttcttcca ggttatcggg atgtacctca
    43741 actagtaaaa catggtttac ctttatctca agctttttta tggtctcgag gacatgctgc
    43801 aggtaattct tatccggaag aattgaaagc tctaccacca caaattatca tcggtgcaca
    43861 atatgtacaa gcagccggtg ttgcattagg tttgaagaaa cgaaacaaga aaaatgtcgt
    43921 cttcacttat actggtgatg gcggttcatc acaaggtgat ttttacgaag caatcaattt
    43981 cgctggcgct taccatgcaa atgccgtatt ttatatccaa aacaacgggt atgcgatctc
    44041 tacacctcgt gaaaaacaaa ctgcagcaaa aacacttgca caaaaagctg tcgcagctgg
    44101 gattcctggt attcaagtcg atggaatgga tccgcttgca gtatatactg tatctaaaat
    44161 ggcacgggaa tgggcgatca gtggaaatgg accagtccta atcgaaacac tgacttatcg
    44221 ttatggacca catacactat ctggtgacga tccaacccgc taccgtacaa aagacacaga
    44281 tgacgattgg cagaaaaaag atccattgat ccgcttccgt aaatatttga cggaaaaagg
    44341 actttggtct gaagaaaaag aagaacaagt catcgaagca actaaagaag aaatcaaagc
    44401 agctattgct gaagccgaca aagtaccaaa acaaaaggtt tcagatttct tgaaaaacat
    44461 gttcgaagta tctccacaat caatcaaaga acaaattgca atttatgaag cgaaggagtc
    44521 gaaataatca tggcacaaaa aacaatgatc caagcaatca cagatgcctt agcacttgaa
    44581 ctagaaaatg acgagaatgt cgttgttttt ggtgaagatg ttgggaaaaa cggaggagtt
    44641 ttccgggcaa ctgaaggatt gcaggaaaag ttcggagaag accgagtatt cgatactcct
    44701 ttagctgaat ctggaatcgc aggattgagt ttcggtcttg cgttagaagg atttcgtcca
    44761 gttcctgaaa tccaattctt tggttttatt ttcgaagcaa tggacgaagt agtagctcaa
    44821 atggctcgta caagatatcg aatgagcggc actagaaatc tacccatcac aattcgttcg
    44881 ccatttggtg gcggtgttca tacaccagaa ttgcattcgg ataatttaga aggtttgatc
    44941 gctcagtctc ctggtattcg agtagtgatt ccttccaacc cttatgatgc aaaaggacta
    45001 ttgatttctt ctattagaag taacgatccg gttgttttcc tagaacacat gaaactatat
    45061 cgctcattcc gagaagaagt gccggatgaa gcttacgaag ttcctttgga taaagcagca
    45121 gttactcgtg agggaacaga tgtatctatt atcacatacg gagcaatggt gcgtgaagca
    45181 atcaaagcag cagacaatct tgcaaaagaa aatatatctg ttgaaatcat tgatcttcgt
    45241 acagttgcgc cattagatgt agaaacgatc attcaatccg tggaaaaaac tggtcgcgta
    45301 gttgttgtcc aagaagcaca acgacaagcg ggcgttgccg cgcaagtagt ttctgagatc
    45361 tcagaacgcg ctatcctttc attagaagcc ccaatcggac gagtttctgc acctgataca
    45421 gtcttcccat ttggacaagc agaaaatgtc tggttgccaa atgcaaaaga tatcgaagac
    45481 aaagtaaaag aaatcgcgga attttaagaa aagaagggac gaaacaaagt catggcttat
    45541 caatttaaat tgccggatat cggtgaagga atcgcagaag gtgaaatcgt caaatggttc
    45601 gtcaaaccag gagatacgat caatgaagat gatacattac tagaagttca aaatgacaaa
    45661 tcagtagaag aaatcccgtc acctgttaca ggaacggtta aaaatgtgat tgttccagaa
    45721 ggtaccgtag caaatgttgg ggatgtatta gtagaaatcg atgcaccagg acacgaagac
    45781 aatgaagggg acagtggtgt agccgcagaa tcccaaactc cagctaaacc ggctgctgaa
    45841 cccactgttg atacagaatc agctggatcg tcaagcgaag gagtcttcca atttaagtta
    45901 ccggatatcg gtgaaggaat cgcagaaggt gaaatcgtca aatggttcgt caaaccagga
    45961 gatacgatca atgaagatga tacattacta gaagttcaaa atgacaaatc agtagaagaa
    46021 attccgtcac ctgtcacagg gacagttaaa aacgtgattg ttccagaagg cactgtagca
    46081 aatgttgggg atgtattagt agaaatcgat gctcctggac acaatagtgc accgtcaact
    46141 agtgcgccat cagctgaagc accaaaagaa aaagtggaaa catcaggttc agcaagtgtc
    46201 gttgaagctg ccgatccaaa taaacgtgta ttggctatgc catctgttcg tcaatttgcc
    46261 cgtgaaaaag atgtagatat ctcacaagta actgcaactg gaaaaggtgg cagagtaaca
    46321 aaagaggaca tcgaaaactt cttggcaggt ggtccaagca gtgcaccagc gaaatcagaa
    46381 gcgccagaag cggcagcacc gaaagaagca gctcctgcag cagaaagcaa accagctgca
    46441 ccagcaaaac cattcaagtc caacttagga gacttggaag aacgtgtagc aatgacgcca
    46501 acacgtaaag ctatcgctaa agcaatggtc aacagtaagc atacagctcc acacgtgaca
    46561 ttgcatgatg aagtagaagt gtctaaacta tgggataatc ggaaacgatt taaagaagtt
    46621 gctgctgcaa atggtacgaa acttactttc ttgccatatg tcgtgaaagc tttaacagcg
    46681 actgttaaga aatacccagt attgaatgca tctatcgacg atgcaaacca agaaatcgtt
    46741 tataaacatt actacaatat cggtattgca actgatacgg atcacggact atatgtacca
    46801 aatgtgaaag atgcagaccg caaaggaatg tttgctattg cagacgaaat caatgagaaa
    46861 gcaaaattag ctcatgacgg caaactatct gcagaagata tgcgcaatgg aacaattacg
    46921 atcagcaata tcggatctgt aggtggcggt tggtttacac cagtcatcaa ctatccagaa
    46981 gtagctattt taggtgtagg aacgattgca caacaaccaa tcgtaaatgc tgaaggcgaa
    47041 atcgttgtcg gtcgtgtgat gaaactgtca ttaagtttcg atcaccgtat cgtagatgga
    47101 gcaactgcgc aacaagcaat gaataatatc aaacgtcttt tagctgatcc agagctatta
    47161 atgatggaag gatgataaac aaatggtagt tggagatttt gctgtagaat tagatacagt
    47221 tgtaattggt gcgggacctg gtggctatgt agctgccatc cgcgctgctg aaatgggaca
    47281 aaaagtagca atcattgaaa gagagtatat tggcggtgtt tgtttgaatg tcggctgtat
    47341 tccttcaaaa gcactgatag ctgcaggaca ccattatcaa gagtcactag attctgaaat
    47401 gtttggagta actagtgaaa acgtcaaact agattttgca aaaacacaag aatggaaaga
    47461 aaacaaagtt gtccatacgt tgacttctgg tgttggtttc ctattaaaaa aacataaggt
    47521 tgaaacaatc gaaggagaag cgttcttcgt agatgatcac acactacgcg tcattcatcc
    47581 agattcagct caaacttatt cgttcaataa cgcaatcatc gcaacaggtt cccgtccaat
    47641 cgagattcca ggatttaaat tcggaggacg tgtgttagat tctactggcg gattagcatt
    47701 gaaagaagta cctaagaaat ttgtgatcat cggtggcggt gtgattggtg ccgaattggg
    47761 cggagcgtac gcaaacttag gagcagaagt aacgattctt gaaggctcac cacaaatttt
    47821 accaacttac gaaaaagatc tagtaaaatt agtagaagat gatttcaaga aaaaaggcgt
    47881 aacagtcgta acgaatgcaa tggcgaaaga agcagtcgat aacggtgaca gcgtgacagt
    47941 gaaatatgca gtggacggga aagaagaatc cgttactgct gattatgtga tggtcactgt
    48001 aggtcgtcgt ccgaatacag atgatatggg cttagaacaa gcgggtgtcg aagtaggaga
    48061 acgaggattg atcacggtgg ataaacaagg acgtacgaac gtacctaaca tctatgccat
    48121 cggtgatatc gttccagggg cggcattagc acacaaagcc agttatgaag caaaaatcgc
    48181 tgctgaagca atctctggca aaaaagtagc tgtagattat aaagcaatgc cggcagtagc
    48241 cttcactgat cctgaattgg cttctgtagg tatgacgatc aaagaagcaa aagatgctgg
    48301 tatcgaagca aaagcatata aattcccatt ttcagggaac ggtcgggcat tatcgcttgg
    48361 taaaacagaa ggattcatcc gtctagttac gacgatcgaa gataacgtgc ttattggcgc
    48421 gcaaatcggt ggagtcggcg caagtgatat ggtctctgaa ttagctttag caatcgaatc
    48481 aggaatgaat gctgaagata tcgccttgac gattcatcct catccatctc ttggcgaaat
    48541 cgtcatggat gcttcggaat tagcattagg tttaccaatc catatttgat ttgaataata
    48601 caggcgaaac gcaactgaaa aatcagttgc gttttgtttt ttagaattat caataatatt
    48661 attatgtaaa cttaaaaata tgacaacttc ttggtttatt accacttcct atgctacact
    48721 gaagaggaat atgaaatagg agagttgaag atgcaagaat atcagtatcc acttgattta
    48781 gattggacaa cagacgaaat ggtcattgta atgaagatgt gggaagatct ggaacgtgcg
    48841 aatgaaaaag ggataaacaa tgaagctttt ttgaagactt atcagcagtt taaaactgtt
    48901 gtcaaatcca ttggtgagga aaaacgattg ggacgggaat ttgaaaaagc ttccggttat
    48961 tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa acaaaacttt gagaatgaac
    49021 gggtgagata aatggaacag atgattgatc atatcaagtc ctggcttgaa gaagctggtg
    49081 agaaaatcaa atcttctcaa caaacattaa caatctcgca aaaatcaaat cgaacggatc
    49141 tagtaacaaa tgtcgatcga aacatccaga attttctaat cgaaaaaatc cattcctttg
    49201 atccagaggc gaaaatattg gcagaagaag aagggtgtaa tcaattatcg gatctcgatg
    49261 gaagagtctt tatcatcgat cctattgatg gcaccttgaa ttttgtgatt gaaggggaaa
    49321 atttttgtat tatgctcgct gtttatgaat ccggcgttgg aaagcttggt ttcatttatg
    49381 acgtgatgag aaatgaactt ttttggggtg gaaaaggaat aggcgtgtat aaaaatgatg
    49441 agccattgcc tagaccagca aataaatcct tatcggaagg cttacttggt gtgaatagtt
    49501 atcttttcgg tcataataaa tatggaatcc gagaaattgc ggaaagcagc atggggacac
    49561 gtatgtacgg atgcgctggt cttgaactag tggcaatctt gaaaggaaac cacattgctt
    49621 atatttcaaa ccttagccct tgggattatg cagcgggaaa tgtcttattg gaagagtttg
    49681 gtatggtaac aagcggtctt tttggtactc ctttgacatt tgaaaaaagg gaatactatt
    49741 tagcagggac ttcttgtgca tatgcagaaa tcgcagacct tttgaaaaaa gaaggctgaa
    49801 aaaaaggtag aaatcattca caaaaatgat aaaaacgagc aaaagattaa ttttctcgaa
    49861 aaaatgaaaa cattttacta tatttcatga aaaaagtctg ctataattag cagtcgagta
    49921 aaatttgccc tgtcataaag aaccaataaa aaaggagtta gttacattga attatagaaa
    49981 cgatatccgt aacgtcgcaa ttattgccca cgttgaccac ggaaaaacaa cattagttga
    50041 tgaactatta aaacaatcag atacattaga cgcacatacg caattgcaag aacgtgcgat
    50101 ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt ttagccaaaa acacagctgt
    50161 agactataaa ggtattcgcg taaacatcat ggatactcca ggacacgcgg acttcggtgg
    50221 cgaagtagaa cggatcatga aaatggttga cggcgttgtg ttggtcgtag atgcgtatga
    50281 aggaacaatg cctcaaacac gttttgtatt gaaaaaagca ctggaacaac atatcacacc
    50341 aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt cctgagcatg tcgtagatga
    50401 agttcttgaa ctattcatcg aattaggagc agatgatgat cagttagact ttcctgtgat
    50461 ttatgcatct gcactaaatg gtacgtcaag cttgtcagat gatccagcag atcaagaacc
    50521 aaccatggca cctatttttg atacaattat tgaaaaaatc cctgctcctg tcgataacag
    50581 cgacgaacca ttacaattcc aagtttcatt acttgactac aacgattatg taggtcgtat
    50641 cggaatcgga cgtgtattta gaggaacaat caaagtagga gaccaagtag cattaattaa
    50701 attagacgga acagttaaga aattccgtgt aacgaaatta tttggtttct ttggactaaa
    50761 acgtctagag attcaagaag caaaagctgg agacttgatt gctgtctcag gtatggaaga
    50821 tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa gatgcgttac ctatccttca
    50881 cattgacgaa ccaacattgc aaatgacttt cttagttaat aattcaccat ttgccggtcg
    50941 agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt ttgatggcag aacttcaaac
    51001 agatgtatcc ttacgagttg aaccaacaaa ctctccagat gcatggacag tttctggtcg
    51061 tggagaattg catttgtcta tcttgattga aaacatgcgc cgtgagggat acgaattgca
    51121 agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt gtaaaatgcg aaccgtttga
    51181 acgcgtgcaa atcgatacac cagaagaata tatgggtagt gttatcgagt ccttgagctt
    51241 acgtaaaggt gaaatgcaag atatggtcca tacaggaaac ggacaaatcc gtttgacatt
    51301 cttaacacct gcccgcggtt tgattggtta ttcaactgaa ttcctttcaa tgacacgtgg
    51361 ttacggaatc atgaaccata cttttgacca atatctgcca atgcttcctg gtcaaattgg
    51421 cggacgtcac caaggggcgc ttgtttcgat cgatacagga aaagcaacga cttattctat
    51481 catgagtatc gaagaacgcg gtacagtctt cgtagaacca ggtacagaag tatatgaagg
    51541 aatgattatc ggggaaaaca gccgtgataa tgatttgact gtcaacatca cgaaagcaaa
    51601 acaaatgaca aacgtacggt ctgctactaa agaccaaaca tctgtcatca aaaaaccaaa
    51661 acaattgacg ttggaagaat ctttggaatt cttgaatgat gatgaatatt gcgaagtaac
    51721 accagaaagc attcgcttga gaaaacaaat tttagagaaa aatgctcgtg aaaaagcatc
    51781 taaaaagaaa aaataatctg taaaaggatg aagagagaaa ccagcttgtt ataaaaaata
    51841 gctggtttct ttttttgtcc aactcttttc tgtggagaag tcatctttgt caatggacat
    51901 aaattcagaa aatacgccga aagacgttta aaagactttt aaaaaactta tttctagaga
    51961 gtttttgttt cataataaga taatgtttca cgaaaggaga tcaacttgtc cttcgaagaa
    52021 aatcgaagta aaatggcaag agaaaggagc gggaagatga acgacttatt aaaacaatac
    52081 tttggttatg atgagtttag acctggtcaa aaagaaatta tccagaaagt cattgatcag
    52141 gaaaatgtat taggaatcat gcctaccgga agtgggaaat caatctgtta ccagcttcca
    52201 gctttactat tagatgggct tactgtggtt gtgtcccctt taatctcttt gatgaaggat
    52261 caggtggacg cggcgaatca attaggtata cccgccactt ttatcaatag ctcgttagat
    52321 ggttacgaaa cagctagacg cttccaagaa atcgatagac agcagtaccg tttactttat
    52381 atcgctcctg aacgttttat catgccggat tttatccaag caatgaagcg atggaacgtc
    52441 cgtatgatcg ctattgacga ggcacattgt atctctcagt gggggcatga tttccgccca
    52501 agttatttac agatggcgaa ccagctagat caactgccaa atcgtcctgt aattgttgct
    52561 ttgacagcta cagcgactgt gcaagtagct gctgacataa aaagactttt aaaaattcca
    52621 gaaaacaacc atattcagac tggttttgaa cgagaaaatc tacgatttca agtaattaaa
    52681 gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga tcaacaaaaa tcaatctggt
    52741 atcatttatg cagcaacgag aaaagaagta gaccgtctct atcatttgct taaaaaattc
    52801 gatttttccg tgggtagata tcatggtggt ctaaatgaga atgagcgaac agaaatgcaa
    52861 gaagcctttt tatacgatcg cttgcagctg attgtagcga caaatgcatt tggtatgggt
    52921 ataaataaaa gcaatgtccg ttttgttatc cattatcaaa ttcctggctc tctagaagca
    52981 tactatcagg aagctggtcg ggcgggaaga gatggtcttt ccagtgaagc catcctgctt
    53041 ttctctcctc aagatatcca agtccaaaag ttttttatcc agcagtccca aagagaagaa
    53101 gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga cagaatacgt gtatattgag
    53161 tcctgtcttc agcaatacat cctaaactat tttggcgaga caagcagtcc gtgcaaccgt
    53221 tgtggtaact gtttagatga cagagaaata gtagaagtaa cgacagaagc acagatggtt
    53281 ctttcttgct taaaacgtat gggtgagaac tatggtaaac aaatgctgat gaaagtctta
    53341 gcaggatcga aagaacaaaa gctgcaagct ttaggttttg gacatttatc tacctatgga
    53401 ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa ttgaatattt gatatcgaat
    53461 ggctatcttc tgaccatcaa tggtgagtat cctgttttga aagtaacaga aagagggata
    53521 caagtattga aaggacaaga atctgtttat cgaaaggaac ccaagaaagt ccagcagcta
    53581 tcggatgaag aaaccgatac actatttgaa gtgctgcggg aactgcgaac ggatctagca
    53641 tcagaagcag gcgttccccc gtatgtcgta ttttcagatt ccacattgaa agaaatgagc
    53701 cgaatacgtc catcaagcag attggagatg cttcagataa aaggagttgg ccaaagcaag
    53761 cttgataaat atggcgaagc ctttttatct cggataaaaa atgcggactc taacgttctg
    53821 gataagtaga aagaacgaat acgtaaaaat gaataaaaac aactggttaa atctgttttt
    53881 tttgctcctt atgaaaatca aatatgttat aattaaggaa ttagaatggg aggtaagcac
    53941 agatggattc tttgtcgaaa aaacttgcga tccaagtatt aaatgatgaa gcagaacgga
    54001 tcaaacgatt aatccgaaat caaaacaaca gtctctgtat ctcacaatgc aaagccttcg
    54061 aagaagtggt agatacacag atgtacggct tttcccggca agtcggtttt gctgtccgta
    54121 taggagtaat cggaaagaac gagggccagc tgatgttaag tgagctagaa cgagaattga
    54181 atcagttata tagtgaggtt taccaagaaa tttatgataa aaaagaagtt ggcaaggagg
    54241 aatagacttg cctaagaaag tgaaaaaaag gcatctatta gactatagca tcttgattcc
    54301 ctatttaatt ttatgtgtat tgggattgat catggtatat agttctacgt cgtatctatt
    54361 attagaaaac ggacaaaatc cgtccgcatc agtcattaac cagagtattt tttgggtgct
    54421 tagcttgatt gctatcgcac tactttataa aatgaaaaca gatgttttga aaaaccagcg
    54481 tttgatcatg gcagccatcg cagttttgac gattttgctg ctgattgttg tgttttttgg
    54541 taaggagatc aatggggcga aaggttggct gcaaattgca ggattctcga tccaacctgc
    54601 cgaataccta aaaatcattt ctatctggta cctgtctctg actttatcta aaagacaaaa
    54661 cagtgtacaa aaagattttc ttgggactgt gaaacgtcca cttgcaatgg ttatcggact
    54721 tactgcatta gtcgctattc ttcccgattt tggtaatgcg gcagttatct ttttgattat
    54781 tttggttctg cttttagcca gcggagtcaa ttatgtgtat actttgattg taggagtagg
    54841 aggcttttgt ctaagtactt ttacgatctg gctgatcaat atcacaaacg gaaagatctt
    54901 acccggacgt ttacagtata tctataatcg atttgctatt taccaaaatc ctttctcaga
    54961 cgaattaaat aaagggcatc aactagtcaa cggctactat gccatgttca acggaggact
    55021 atttggcagg ggattaggta acagtatcca gaaaaaagga tttttacaag aagcacaaac
    55081 cgattttatt tatgcaatcg tagtggaaga actgggagtg attatgggga ttttgatatt
    55141 agcgttgcta ttctttatga ttgtgcgcat tgtccttgta ggtatccgtt caaaagatcc
    55201 gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt ttgatccaag tatttgtcaa
    55261 tcttggagga attactggcg ttattccttt gacagggatc acgttcccat ttttaagtca
    55321 aggaggctcc agtttgctga tgctttcgat ttgtgtcggc tttgtcctca acatcagtgc
    55381 ggatgaaaaa cgcaagagct taggtttata ataaaaaaag atgtaggagt ggttcgatga
    55441 aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg tgtatttcgt gcttgtacag
    55501 aattaggaat taaaactgtt ggaatctatg cagaagaaga tgaatattcc gtacaccgtt
    55561 tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa accgattgat gcgtatctag
    55621 atattgaagg gatcatttct atcgcgaaag aatgcggagc tgacgctatt catccgggtt
    55681 acggtctttt gtcggaaaac ttgaattttg cgcaacggtg cgaagaagaa ggcatcattt
    55741 ttgttggacc caaactgcat catttagata tttttggaga taaaatcaaa gcaaaagctg
    55801 cagctatcga agcggggatt gcttcgattc caggcacaga tggaccaatc gcttctatag
    55861 acgatgcctt ggagtttgcg aaacaatacg gttatccgat tatgatcaag gcagctctag
    55921 gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa gagtgcaaga gagggctacg
    55981 aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga tgaagtgtat gttgaaaaat
    56041 acatcgctaa tcctaagcat atcgaagtac agattctcgg ggatacgcat ggaaacgtca
    56101 tccatctttt tgaaagagat tgttctgttc agcgccgtca ccaaaaagta gtagaagtag
    56161 cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat ctgccaggca gctgtccaat
    56221 tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga atttcttgtt gaaggagatg
    56281 atttttactt tatcgaagtc aaccctcgcg tccaagtgga acatacgatc acagaaatga
    56341 ttacagatat tgatatcgta acaacgcaac tgttgattgc tcaaggtttg gatctgcata
    56401 aagaaatcgg attgcctcag caagaaggaa ttaaattaaa tggttcagcc attcaatgcc
    56461 gaatcactac agaagacccg ttgaataact ttttaccaga cacagggaaa atcgatacgt
    56521 atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg aaatgcttac gcgggctatg
    56581 tagtgacacc gtattttgat tcattgctag taaaagtatg tacgcatggt gctacctttg
    56641 aaacagccat tcagaaaatg gaacgttgct tgaaagaatt tagaatccgt ggagtgaaaa
    56701 caaacattcc atttatgctc aatgtcatca cacatcctga attccaatca ggaaatgcaa
    56761 aaacaacctt cattgatagt acagcaacat tatttgaatt tcctcgacta cgagaccgcg
    56821 gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa tggttttcca ggtatcgaaa
    56881 gtggcgaaaa accattttat gaagaaccac gtatgcctaa agatctaatc acaagaactg
    56941 attacgtaac ggctaagaat gtattagatg cgaagggtgc cgatgctttg gtcgaatgga
    57001 tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt tagagacgcc catcaaagtc
    57061 tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat tgcacgtttg actggagaag
    57121 gtcttcctga actcttttca agtgagatgt gggggggagc aacatttgat gtggcttacc
    57181 gctttttgaa tgaagatcct tggcaacggt tgagaaaaat tcgttcttta atgcctaata
    57241 cgttgctgca aatgcttttc agaggttcaa atgccgttgg gtattctaat tatcctgaca
    57301 atgtattggt ggaatttgtc aaagaagcag cagcgcaagg aatcgatgta tttcgaatct
    57361 ttgatagttt gaactggact ccacaaatgg aaaaaagtat ccaagctgta cgggatactg
    57421 gtaaaatcgc agaggcagct atttgctata ctggcgatat caacgatcca agtcgggcaa
    57481 aatacaatgt tcagtattat aaagacatgg caaaggaact agaacaactg ggtgcgcata
    57541 tcattgcaat caaagatatg gctgggctgc ttaaaccgca ggcagcttat cgtttgatta
    57601 gtgaattgaa agaaacgaca gatctgccaa tccatctgca tactcatgat accagcggga
    57661 atggaatcat cacttattct gctgcttcaa aagcaggagt agacatcgtt gacgtggcaa
    57721 tgagtgcaat gagtgggaat actagtcagc ctagcatgag cagcttgtat tatgcactgg
    57781 tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc acagaaatta aaccattatt
    57841 gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg cttgaatgct cctgaaacag
    57901 aagtatatat gcatgaaatg ccaggaggac agtattcgaa tctacagcaa caggcaaaag
    57961 ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat gtatcatact gtcaacttga
    58021 tgtttggcga tatcgttaaa gtaactccat catctaaagt agttggagat atggcactat
    58081 ttatggtcca aaatgatctt acagaagaag atatttatga aaaaggcgaa acactaagtt
    58141 tccctgaatc tgttgtgact ttcttccaag gagaactagg gcaacccgtt ggcggatttc
    58201 ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc attgaatgaa cgtccaggtc
    58261 ttttggctga atcagtcgac ttcaacgaag taaaaaaaga attggcagaa aaaattggct
    58321 atgaaccaaa acaagacgaa gtattaagct atttgatgta tccgcaagtc tttttggact
    58381 atcaaaaagc atataatcaa tttggcgatg ttacattact ggacacacca acatttttcc
    58441 aaggtatacg tttgggtgaa acaatcaatg tacaaatcga acgcggaaaa acgcttatca
    58501 ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa tcgcgtactg ttcttcaatt
    58561 tgaatggaca aagacgtgaa atcactatca aagataattc gatcatcagt acggtccaaa
    58621 cccgattgaa agctgaaccg acaaaccgag aacaaattgg agcaacgatg tctggttctg
    58681 ttttggatgt attagtgaaa aaaggcgata acgttaaaaa aggtgatacg ttgatgatta
    58741 ctgaagcgat gaaaatggaa acggccatcg aagcacgttt tgacggagaa gtcgcacatg
    58801 tatatgtgtc ttcaggagac actatttcgt ctggtgacct tttgattgaa gtgactgaga
    58861 aataataagc gaaaggtggg gaaacatgaa aagactgggt ttgttttggc tgattctgct
    58921 attagttatt gtggcattct acatggaacc agtcttgttt ccgccaaaac ttgaagtgaa
    58981 ggaaaactct gatcaacaaa tatcaaacca aatgacaacg tggaaatacc aggagcttaa
    59041 agcaactggt ttttctactt atatcggaaa accgatcgat cagctggaaa aggattttgg
    59101 ccaagcctat gatcgattaa ctagcggatt tggttttgaa acaagatatt atgaaaataa
    59161 agctagtcat ctcacatttg aagcgaacat ccaagacagc agagtcacaa ctgttaaagt
    59221 actgcaaaca gggcctgctg atatcgcgcc gttttattta gggatgacga tgcaagatct
    59281 tacgaaaatc actactatct atactaattt cacttttgag taccatgata cagaagttgg
    59341 aatcgaacta atggaagagg atatgaatta tcgtccgcta attgcttttg ataacgacac
    59401 ttttgctatt ttattttttg atcaaacaac aaacggccta ttttcagttg tgtatttaga
    59461 taaagatagt cttttgaaac tattgccata tcaagtgttt ggtgaaggcc ttccacatta
    59521 tcaagcagat aaatcggctg attgggaatc aatcaatcgc gttaaagaac gaaaaagtac
    59581 gactttatta aatatgctta gaagagaaga cgaacttccc atatacaatc aaacacttgc
    59641 ctttcaaaac cacagtcagc tgctactaca tgattttttg acaaggcctg aggaaatatt
    59701 gtcagaggaa cgtatggctg attggcaaaa atcgctaggc agtgctcaaa cgacagtgaa
    59761 attctcactg acgaatgatg aattggagaa gttaatcagt aaacaaaaat tagaaaatac
    59821 tagtgggcta tttattcatc cggcgattga cgctactttt tcgtttttat attggtacag
    59881 cgatccttac aaccatgacc gtttcatgga cccgggaacg gattctgtag gcattgcttt
    59941 ttctaaagaa aatatgttag tcttattgca agaagaaaac ggagaaagta gtgatagcag
    60001 tgatcgttaa cgaagagctg tttcaactgg aggatcaatg cctccaagtc gctgaaatga
    60061 taaaaaagag taaaacgatg gcttcttatc ttgaacataa acagtcaatg gaagaatctg
    60121 caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa agaagcattt gaaaatgtag
    60181 cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa acgagctgtt cgaaaagcaa
    60241 aaagacagct tgatatggat gagcatgttg cagctttccg atatagtgaa acacagctgc
    60301 aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga agtttctaat gaaatcaaag
    60361 tagatgcagg tagcccgttt tttgaaacga aaaaacactt tggatgcgga ggtaattgtc
    60421 atgcaattag acaatagtga atttgaaata acaaaacgac gcgggctgat cgtatgggtc
    60481 tacagcttaa aacaattaaa gaatctgaaa aagttcggct tgttgcatta tgtctcaaga
    60541 agaatgaaat atgtcgtgct ttacttaaat gaagagacat tggaagaaac agaagaacga
    60601 atccgtaaat tacattttgt tcgaaacgta gaacgttctt accgaccgga tatcgaaatg
    60661 aattttgccg aaaaaatagg aaacaaaaca gtattaaaag acgaaggttt cgaaattgaa
    60721 gagctaaata cagaaatccg attagctgaa catctttaaa aatctcttat gagcagtttt
    60781 taaataaaaa ataacttgga taacgatcga tttttgatca ttgtccaagt atttttttgg
    60841 tatactagta ccagtaccat ttctaaggag tgagtagatg cggataatat caggaaatta
    60901 tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc agaccaacta cagataaagt
    60961 aaaagaatcc atattcaata tgattggacc atattttgat ggagagacag tattggatct
    61021 gtttgcagga agcggtggtt tagctatcga agcagtatct agaggctgca gtcatgcggt
    61081 atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa gaaaacatcg aaattacgaa
    61141 agaaccagag aaatttacca caataaaact tgatgcggac aaagcaatcg aacggctagc
    61201 agcggagaaa caacaattcg actacttgtt tcttgatccg ccatatgcaa aacaaaaaat
    61261 aatcgatcaa atccataaaa tggaagaact gggacttttt tctaaaaccg cagttatcgt
    61321 atgcgaaacg gataaaacag tcgaattgcc agaaactgtg gctggattcc atcaaataag
    61381 aaaacagatt tatggcataa cggccgtaac tatctataga aaagagggaa atcaatgaaa
    61441 cgtgcattat tcccaggaag ttttgatcct tttacaaaag gtcacttaga tacggtggaa
    61501 cgtgcggcta aattattcga tgaggtagta attggtgttt ttatcaatac ttcaaagaaa
    61561 agtttgtttc ccccagaaga aagaatgaca ttgatcacaa aagctgtttc tcatcttcct
    61621 aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag aaacagcaaa agagattggt
    61681 gcagatgcgt tgatcagagg catacgaagt atcaaagact ttgaatatga acgagagatt
    61741 gctcagatga atcatcatct atatgatgaa ttggaaactg tcttcttatt agcaaaacca
    61801 gaatatagtc acgtgagttc cagtatttta aaagaagtac tgcactttgg cggagatgtg
    61861 agcagctact taccgccagt gatcaacgaa gctttagctg gaaagcgtga atcaaatgaa
    61921 agctaaacaa tggttgaaaa cagtactgat tatcatattt ggaatgttga tcattggatt
    61981 tgtagtagtt cccttgccgt attatgtaga atcaccggga gccacgatcg atctgaaaga
    62041 actaattacg gtcaatgata aggaagataa aggttctggg tctttctctc ttacctctgt
    62101 tggtatcagg caagctacag gtttcttagc tttaaaagct aaaatgaccc cttttgaaga
    62161 attgatcagt gaagaagaat tgactggtgg ggcgacaaat gaagaatata atcaaatgca
    62221 acagtattat atggattctt cccaaaatgc agcaatcgaa caagcgttaa aacttgcaga
    62281 caagccttat aaaatggaat tcaaaggcgt atatgtcatg agtgtagaga aaacatcgaa
    62341 tttctttggc aagctttcag ttggagatac agtaacaaaa gtagacggga aaagctttca
    62401 aagtacagaa gcatttatgg attatgtaaa aagccaaaaa gttggacaaa caatcgaaat
    62461 cagttatttg cataatggtg aagaaaaaac agcttctggt gatctgatcg agttaccaac
    62521 agataaaaag gcaggaatag gtatcacgct tactgaccat accgagatcg aatcagatac
    62581 agatgttcgt attaattctg gctctatcgg tgggccatca gcgggattga tgttcacttt
    62641 agaaatctat gaacaagtca ctggtaaaaa cttgcgacat ggtaagcaaa ttgcaggaac
    62701 tggaacgatc aacagtgaag gagaagttgg ccgaatcgga ggaatcgata aaaaagtggc
    62761 aagtgcggat aaagctggcg tagagatttt ctttgcacca gatgatacga tttcagacga
    62821 tgttaagaaa gaatatcctg aaataaaaac caactacgaa gaagcaaaag ctgccgctaa
    62881 aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca gtacaagaag cgcttgacta
    62941 tctagagaaa atgaaatgaa actaaataat taaagagggg ttgtgacacg agttctgtca
    63001 caacccctct ttttcgtcaa aaatcttcac gataaaaata aaaacaaaag aaaaaattga
    63061 cgtaattaga tgttgaaggg acgattttgc cagagctcac tgtttcttca attttaaatg
    63121 aaaggagtaa tgaatgattt ttactggtca ttctttgaaa aaagaacaat gatagaaaaa
    63181 atcagcaaga aaatactagc ttcacccaaa ttgatcattt ggatgtctat gatagttctt
    63241 atcttttgta tcgcaagtgg ttttttatta tcaaatttgc tattttatag agaaacagaa
    63301 gagactgctg caaaagaaat actttcttcg tttagttcca gtggagaaga tccatcagct
    63361 gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca tgtacaccga tatcaaagga
    63421 agtgtgaagg agcctggtat ctattccttt tcttcagaag aacgggtcta tgatgtacta
    63481 aagcgtgcag gtggattatt agaagaagcg gatagcgatc gaatcaactt ttctgcgaag
    63541 atagaagatc agcaagtcct atatatccct gctgtaggtg aagagctgcc agaacatctt
    63601 gatcagttgg catcctcgga aggaaaacag cctacagcag attccgagcc tcccaaaata
    63661 aatatcaata cagccactcc atcagaactt cagcagattc ctgggatcgg atcagtcaaa
    63721 gcgcaagaaa tcattcgatt tagagaagaa aacggatcct tccagaaagt ggaagacctg
    63781 caggagattt ctggtatcgg tgaaaagaca gttgaaaaac taaaaaactt cgttacaatt
    63841 aaatagaatg attcaaaatc gactggcacg agcaataaat ggacgctaat ggatactaag
    63901 aataatccaa ttctaagagc ttggcaggac gaggaggtag tatttttgga taggaaatag
    63961 cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg aatgtctatc tccaaaataa
    64021 aatatctggg attcatatta tgaatacggc tgtcagcggc aataacagtc aagctctacg
    64081 agaacgcttt gccaaagatg tacttagcta tacagcagat aaagtattta ttctcattgg
    64141 gacgaatgat ctagcagagc ataaacaact ttctaaagaa acgtaccaaa aaatctgcag
    64201 tggatgatag ttcagctgaa aaaagactat agttctgatc atgctttctt tatcacgcca
    64261 ccagcagtcg atgagacaaa acaaaaatat cggaataaca agttgatcaa agaatatatc
    64321 tctgttctca cagaagtagc aaaacgccat gattgtgaag tatcgatttt tatacggaat
    64381 ttcttgaaca aatggattcg aagaattttt ataatgggct aatggatgac ggacttcatt
    64441 tcggcagtga agggtaggat cttttgtcag acaggatgat tcgttatatt taaagaatat
    64501 tttgtttaag caatgagtac tttttaatca acttgaaaaa acggaggaat ataaaacatg
    64561 ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa aagttactga taagtcacat
    64621 aatgaacgca ttccttggga ccagtacttt atggctcagg cagtactgtt atctttacgt
    64681 agtacgtgta cacgtttaga agtaggagcg acactcgttc gagaaaaaag gatcattgct
    64741 ggcggctata atggagcagt cagtggagat gtccactgca tagatgaagg gtgttacgtc
    64801 gtggatggac actgcttgcg aacgatccat gcagagatga atgcactcct acagtgtgca
    64861 aaacttggta tcccgacgga aggttcagaa atctatgtca cacattttcc atgtttggcc
    64921 tgtacaaagg ctttgttgca agctggtgtg aaagggatca attatctcca tgactatcga
    64981 aatgatcctt atgcccaagc gttgatcgaa caattaggtg tatctgtaca ccaagttgct
    65041 ttagactcac agcatttttc aaaacttcaa aatgaattaa caggaaaaca tgtctagcgt
    65101 tttagaaagg catgttcgaa atcggttgat ttttccggca attacgtcga tgattatctg
    65161 tttgttgtat tacactgaca attggggtta tcgtttgctt cttgccggaa tttttttatg
    65221 gttttcgcta aaagaaggac gttttatcgc gtacttgtgt attttgtgta gcataacggt
    65281 atccatttct tataggctac aagagtattc atttttagag gaaaaaacaa catctgcgca
    65341 aattactgta tttacagata cgattcgtgc aaatggtaat tttttgacaa tggaaggaag
    65401 gttatcagag aataaacaaa agctccagct ttcttatcaa gtaaacacag aagcagaatt
    65461 gaaaacatgg cttaacttcc gaggaaaggg aatagaattg actgctgaag gcgagttcat
    65521 ctctccagca aaaaagcgga atctttataa ttttgaccaa gaagcttact tcaggaacca
    65581 tagaatttct ggacggttcc aaattcaaaa gattgaacag atcaccatgc aaaatcactg
    65641 gcgaaactgg cttcagagaa aaagaggatc ttttatctcc tttgtccaat ctgaatttcc
    65701 gcaaaaaaca agtgtatata ttaattcttt attatggggc tataaagatg caggattcag
    65761 agaaccagaa gaaattttta gggagagtgg tttgctgcat ctgttcagtc tatcgggtct
    65821 acacatccaa ttttatttag ggtggctgta ttatttattt cgacgtatcg gatggccact
    65881 acacgtcagt attattccgt tgagtctact aactatctgt tatgtttcta ttgcaggaag
    65941 ttctatttct gttttgcgtg ctagtttgct gtttttattg cgattgctag tcaaattgct
    66001 gaataagcat tattccacca tggacttatt tgcagttgtc ttttggcttc ttttactatt
    66061 tgatcctcga tttctttttc atgcgggtgg acagcttagt tttttcatga gttttttgtt
    66121 tatcctgatt tcttttgatg gagattggtt gaaaaaaacg gcctctcacg tcctgtttac
    66181 tatgatcacg atgccgttga ttgtatggca tttttatgag tggcctattt taggtagtct
    66241 ttttacatta ctgattgcac cggtattcaa gtttcttttt cttcctggtg tgttatttct
    66301 cacactcttc aatcgctggc tgccggaaac tttacttttg ctgatagaag agggattaat
    66361 cttatttgag cgaataatcg atttttcttc agggtcgtct ctcattatag gacgtttgcc
    66421 ccttgtgcta tctgggatgt atattttcgg attactgatt tgtatggata ggttgaaaga
    66481 acaacctgtg aaacacttgt tttatgttgg tcttttttct tttatttttt tgactttgcc
    66541 atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc ggtcaaggag acagtatctt
    66601 tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat actggaggtc agttacactt
    66661 tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct gctgaaagaa atatcgttcc
    66721 atatctaaaa ggtcagggga taagtgtaat cgataaactc atcttgacac acgacgatac
    66781 ggatcatgtg ggagaattac ccacgttatc tcagcatttt actatcaaaa aaatttatat
    66841 cggttggggc gctggacgaa atgagccatt acaaaggcat ttatctgaag ctcaaaaaaa
    66901 tggtactgag atcattgaaa tcaaacatgg ggacaggatc agcggttatt atgatcttta
    66961 tgtattgact ccttttgaaa agggagaggg aagaaatgaa gattcgattg gtctatggat
    67021 ggaatataac aataggcgct ttttattttt aggagatttg aatcaagcaa tggaaaaaca
    67081 attgcttttg atttatccta atctgaaagc cgatgtagtc aaattaggtc atcacggtag
    67141 ccgtacgtca agtaacacag attttctttc ccatattgaa gcaaaacatg ggattatatc
    67201 ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa gtattggaga cactagaaga
    67261 aaatcagata aaaacatggc gaacggatca acaaggaatg atcacctatc gctggcatcc
    67321 agtttttcat cctcatgggc tagtggaaac aatgatagac tagtaattct catctgctca
    67381 tggtaggata atttttgaaa gtggtgagta atttgagtat acaagcagag ttacaaaaaa
    67441 tacgtaaaga aaaacttgca tcttgttact tgattttagg aacagaaaag tttctccaag
    67501 atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga gggcgaagat gacttaaatt
    67561 ttttaagttt tgacatggaa aatgcaagtt tagatgaggt agtagctgaa gcagagacgt
    67621 tgccgttttt cggagatcaa cggttagtat tcgtagagaa tccttatttt ttaacaggtg
    67681 aaaaagtaaa caatggaatc gaacaaaata ctgatttatt gacggcttat ttgaaagaac
    67741 cattagaatc gactgtatta gtattctttg cgccatatga aaagttggat gaacggaaaa
    67801 aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa tgtccaacag ttgaatgaaa
    67861 aagaagtacg tcaatatctg atgaatacct tagagaattc ctcaatcaaa atggaacgaa
    67921 gtgcaattga tttattcttg aggttgaccg acttagattt atccaaaatg atgagagagt
    67981 tacagaaatt ggtcctttat ggacaaaatc aacagcaaat cacggtaaga gaagtagaag
    68041 aattagttcc taaaacgtta gaacataata tttttgacat gacccaatat attttaaaag
    68101 ggaagacgga acaagctttg cgtttatacg aagatttagt tatgcaaggg gaagaaacaa
    68161 tcaaaattaa tgcgattttg ctttctcaat tacgtttatt tttacagaca aaatttcttg
    68221 taaagattgg ttatcagcag gcaaatattg ctgaaacatt gaaaatccat ccatatcgtg
    68281 taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca tttgctggta cagttgtttg
    68341 atcaattagt tgagatggat tatcaaatca aaacgggaca aatcgaaaaa gaattaagtt
    68401 ttcaactatt cgttttgcgc acagggcagt tgttaagaaa tcgataagaa gaaagacgag
    68461 ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt ttgacaaata aaaaaatcgg
    68521 ccgaagccga ttcttttatt tagcgatttt tttgcttaaa cgagatttgt cgcggttagc
    68581 tttgtttttg tggattagac cttttgattc agccatatca atagctttaa cagcttcttt
    68641 atataacgca tctacgttgt cagcacctga agctacagca tcttcaaatt ttttgattgc
    68701 agtacgcata gcgcttgttt gagatgaatt tttcacatta gcgttttcgc ttgtacgaac
    68761 acgtttgatt gcagattcaa tgtttggcat tagtttcacc tccgataact taagttctta
    68821 cgcgtgttac cgcataattc aacattcatt attataccta aaggcagcgt gctttgcaat
    68881 atgaacatga caagtttttt tccttaacag tcttttatta ggattttttt tcagtaaaaa
    68941 cggatagtac aagctgaaaa aaatcaaaaa gacgctattg tgggaggcaa tagcgtcaaa
    69001 ccaaactaga tttttattta tctatttaaa caaagtttga atgggaatgt ttggtactaa
    69061 gttattatat aacaaagaga accgtttgca aagttttttt tgaaaaggga tacgattccc
    69121 tgttgttctt tgtttttcta ttaagaaact gcagttattt tataaccagt atcctttgat
    69181 cagtaaaggc tattctggtg taccgattat tttaatgtat tcatcgatat tttttatcaa
    69241 catttttttt atgaccaact ggttccgacg gttccatttg ccacttactt ccagcacata
    69301 tttatttagg ggcaaacgta agatatattt tgccaaatag cgatctttaa gcaggccatt
    69361 gatcacacgg tcctcaagct gcaagttgat cccagtcagc atcattggct gttcttgtaa
    69421 aacgtacgta tcaattaaaa gaccttcaaa agtttccaca agcgtcttcc tttcttttca
    69481 aagtaacctg aaaacaggca ctaattcttc cattttgata ttcgttgaag tggatgcgat
    69541 cattctttct tttgtccact gtaatctagt ataacaaaaa agagcagaag agccaaaaag
    69601 aaaaaagagc atcgaattct ataggaagtc tcattggcac ggcctaaatt attacaaaac
    69661 attgattaaa aaaagcccct gcatcgcagg agcatcccct atatcgtttt aataggtcaa
    69721 tcaaaaagca gtatcaaaca cattttgtat caaatcagga aataatagat aagctaagcc
    69781 cagaatcaaa agtaccgcta gaagaaacca gccgattgtt cctatgaaat actttaattt
    69841 ttcgaacatt gtatcacctc gttgtcagta tatcacggat cagtaaattg ggggtgttaa
    69901 gaatctccta aaatagacaa aatttgttct attttcctaa agcaaatact tgtaagatac
    69961 agtaagtgat accagaagca atgaccggtc cggcggcgat tcctcttaag aagacaacac
    70021 ctaaaatcgt gccgaaaaca agtgcaacag tgatttgtgg atcagaagca atcaatccta
    70081 cacccttgga agataagaca gccactagcc caccgcaaaa aacagctacc cagccaatag
    70141 gggatttaaa ggcttctaat agatcacgta gaccgatttc tcctgttgcg atagggatta
    70201 gaatagccgc tgtgatgatc gtcaccccaa tattgattcc tttgccttga agccaaggga
    70261 aaaaggaacg ggaggctggt atgagcttca taatcaaaac aaaagctgcc gctatcatca
    70321 gactttgatt tttagctaaa attgctacaa gtaaaataat tcctaaaaaa agccaacttt
    70381 ccatgaaata tattcctcac tttcttcgaa acaatcataa catgttttta gaaagttaca
    70441 aaagtaggaa ttcatccttc aagacctatt cataagctgt ttttttcata aaataaacaa
    70501 aatcataaaa aaacactttg cttatttgac agaagacagc cgcgctttgt atagtggaca
    70561 agacttacat ttttttgaag aatcagaaag atcaggtaaa ggtcttagta aacgcaagcc
    70621 attcagaata accaatattg tgcttccttc gtgtcctaca actccaagag gtagatttaa
    70681 aaattgcagg aagttagaag caatcaacaa gagaataaca agaatcgaaa aaataatatt
    70741 ttgtttgatg atccgccgca tttttaaaga tagccgatga ctgtagacaa atttatttaa
    70801 gtcgcttttc attaagacga tgtctgcgat atccatagcg gcatctgtcc cttgccccat
    70861 agcgacacct aaagaagcgg ctgctaaagc aggcgcatca ttgactccat caccaaccat
    70921 cgcatttact tggtaaagtt cattctcttt tttgactagt tgtgtttttt cttccggagt
    70981 acagcttgca tagtaatgtt caatccctac ttgttcagca atggcctttg ctgtgccaga
    71041 atggtcgccg gttatcatac ttgtatggat gttttgggat ttaaagtagg aaattacttg
    71101 ttgggtattt gcttttggaa tatctagcag acctaaaaca gcaattaact gctgatcttt
    71161 agaaagataa atcacggttt tgccttgatt ttccaaacgt tcgatcattt ccgcaatctc
    71221 tttgctgatt atcatcgtct caggatcata tgcgtgcttg ccaactttcc attgcgcacc
    71281 taaataggta gtttgtaatc caaaaccagt aatttcttct attggtaagt attggatttc
    71341 ttcaggtatt gtgcaatcga aatattgcaa aatcgcatga gcaagtggat gcgtcgtttt
    71401 ttgttccata gcaacaagaa gttgttgtgc caaatccttt tcatcaaaga aaaagctgtc
    71461 agttacaact ggttttcctt tagtgagcgt tcctgtttta tcaaaggcaa tcgcttttaa
    71521 ttcagaaagc ttttctaaat ggataccgcc tttgattaaa atcccatttt ttgcagaatt
    71581 tgaaagagca gctagagaag ctggtgtagc agatgcgaca agcgcacaag gagaagccac
    71641 aactaataat accattcctc gataaaagct ttcatcccaa ctccaaccta ggaaaaagtg
    71701 ggggagaagg atcattagag gaacagcaag caaaatcaat tttacataat tattttcgat
    71761 tcgttcgata aatgaagctg tttgagttgg cgtattctgc gcttcttcta ctaattggat
    71821 aatcttagca aagacagttt cggaactcgt ttttgttact cgaatcgtga tgggatttcc
    71881 ttgattcagc gttcctgcat acatctcatc gcctaaatat ttttctacag gcacagattc
    71941 accgcttaat gcagactcat cgatcgttgc ctgtgtgctt tctaaaaagc catctatcgg
    72001 aacagctgct cctttagcga caaataccaa gttatccacc tttagttgag aaacatctac
    72061 ttcttgcatt tgtccatttt cttttaataa ttgggctttt tgtggttgaa gattcatcaa
    72121 agcagtaatt tctttttgac ttttattagt tgtgtattcc tctaatgcac cgcttaaaca
    72181 aaagatgaat gtcaacattg ctccttcaaa gtaattaccg atcaaacagg caccaatagc
    72241 agctaaagcc atcaataaat ccacattcag tgtccgatca ttccacaatt ctttcaagcc
    72301 ttccgatgtt tgtttatatc caccgatgat aatggcactg atataaaaaa gtggggtgat
    72361 ggtaagagag aatcggtcga agataaaacc gaagatcata aataaaaggc agccgattgt
    72421 tgctgcaaaa gcttttttct gattagtcat cgttattaga cctccttgtc aaaacaagga
    72481 tagcataata aaaatgtaat tgataataat aacagctaaa tgagaacgat aatggttatc
    72541 atttggtgat taagcttgat gttgttttta ttatttataa tgattattat taaagaaaat
    72601 aaaaaaccag aacatttctc aaataagag
//
LOCUS       DAJSBM010000007        71080 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJSBM010000007 DAJSBM010000000
VERSION     DAJSBM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.007"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            161..391
                     /locus_tag="ONT67_000578"
     CDS             161..391
                     /locus_tag="ONT67_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCR2354340.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="ONT67_000579"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="ONT67_000579"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR2354341.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="ONT67_000580"
     CDS             1802..1978
                     /locus_tag="ONT67_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2354342.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="ONT67_000581"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="ONT67_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR2354343.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="ONT67_000582"
     CDS             2593..3060
                     /locus_tag="ONT67_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2354344.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="ONT67_000583"
     CDS             3145..4029
                     /locus_tag="ONT67_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2354345.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4143..4379
                     /locus_tag="ONT67_000584"
     CDS             4143..4379
                     /locus_tag="ONT67_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCR2354346.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="ONT67_000585"
     tRNA            4467..4547
                     /locus_tag="ONT67_000585"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="ONT67_000586"
     tRNA            4554..4625
                     /locus_tag="ONT67_000586"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            4750..4920
                     /locus_tag="ONT67_000587"
     CDS             4750..4920
                     /locus_tag="ONT67_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354347.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5140..5847
                     /locus_tag="ONT67_000588"
     CDS             5140..5847
                     /locus_tag="ONT67_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2354348.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="ONT67_000589"
     CDS             complement(5907..6551)
                     /locus_tag="ONT67_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCR2354349.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="ONT67_000590"
     CDS             6678..6965
                     /locus_tag="ONT67_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354350.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="ONT67_000591"
     CDS             7073..7393
                     /locus_tag="ONT67_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2354351.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="ONT67_000592"
     CDS             7474..8787
                     /locus_tag="ONT67_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2354352.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="ONT67_000593"
     CDS             8784..9503
                     /locus_tag="ONT67_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCR2354353.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="ONT67_000594"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="ONT67_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR2354354.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="ONT67_000595"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="ONT67_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR2354355.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="ONT67_000596"
     CDS             join(13064..13135,13137..14165)
                     /gene="prfB"
                     /locus_tag="ONT67_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR2354356.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT67_000597"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="ONT67_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR2354357.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="ONT67_000598"
     CDS             14940..15833
                     /locus_tag="ONT67_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354358.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="ONT67_000599"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="ONT67_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2354359.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="ONT67_000600"
     CDS             17770..18627
                     /locus_tag="ONT67_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR2354360.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="ONT67_000601"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="ONT67_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCR2354361.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="ONT67_000602"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="ONT67_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR2354362.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="ONT67_000603"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="ONT67_000603"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2354363.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="ONT67_000604"
     CDS             21383..22141
                     /locus_tag="ONT67_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCR2354364.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="ONT67_000605"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="ONT67_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR2354365.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="ONT67_000606"
     CDS             23069..24100
                     /locus_tag="ONT67_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2354366.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="ONT67_000607"
     CDS             24097..24864
                     /locus_tag="ONT67_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCR2354367.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="ONT67_000608"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="ONT67_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCR2354368.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26829..27149
                     /locus_tag="ONT67_000609"
     CDS             26829..27149
                     /locus_tag="ONT67_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCR2354369.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27149..27499
                     /locus_tag="ONT67_000610"
     CDS             27149..27499
                     /locus_tag="ONT67_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR2354370.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="ONT67_000611"
     CDS             27666..28604
                     /locus_tag="ONT67_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCR2354371.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="ONT67_000612"
     CDS             28619..29449
                     /locus_tag="ONT67_000612"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR2354372.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="ONT67_000613"
     CDS             29493..30518
                     /locus_tag="ONT67_000613"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2354373.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="ONT67_000614"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="ONT67_000614"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2354374.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="ONT67_000615"
     CDS             31596..32036
                     /locus_tag="ONT67_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCR2354375.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="ONT67_000616"
     CDS             32039..32641
                     /locus_tag="ONT67_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2354376.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="ONT67_000617"
     CDS             complement(32700..33806)
                     /locus_tag="ONT67_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCR2354377.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT67_000618"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="ONT67_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCR2354378.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="ONT67_000619"
     CDS             complement(35354..37699)
                     /locus_tag="ONT67_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCR2354379.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="ONT67_000620"
     CDS             37978..38880
                     /locus_tag="ONT67_000620"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR2354380.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="ONT67_000621"
     CDS             39093..40868
                     /locus_tag="ONT67_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCR2354381.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="ONT67_000622"
     CDS             complement(40934..42520)
                     /locus_tag="ONT67_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR2354382.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="ONT67_000623"
     CDS             42771..42995
                     /locus_tag="ONT67_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCR2354383.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="ONT67_000624"
     CDS             43137..44888
                     /locus_tag="ONT67_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2354384.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="ONT67_000625"
     CDS             44888..46672
                     /locus_tag="ONT67_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354385.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="ONT67_000626"
     CDS             46998..47849
                     /locus_tag="ONT67_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2354386.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="ONT67_000627"
     CDS             47903..49654
                     /locus_tag="ONT67_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2354387.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="ONT67_000628"
     CDS             49658..49828
                     /locus_tag="ONT67_000628"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2354388.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="ONT67_000629"
     CDS             complement(49868..50770)
                     /locus_tag="ONT67_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2354389.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="ONT67_000630"
     CDS             51001..52404
                     /locus_tag="ONT67_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2354390.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /locus_tag="ONT67_000631"
     CDS             52404..53837
                     /locus_tag="ONT67_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR2354391.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="ONT67_000632"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="ONT67_000632"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR2354392.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="ONT67_000633"
     CDS             55122..56849
                     /locus_tag="ONT67_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCR2354393.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56864..57214
                     /locus_tag="ONT67_000634"
     CDS             56864..57214
                     /locus_tag="ONT67_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR2354394.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="ONT67_000635"
     CDS             complement(57306..58166)
                     /locus_tag="ONT67_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCR2354395.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="ONT67_000636"
     CDS             58258..58686
                     /locus_tag="ONT67_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2354396.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="ONT67_000637"
     CDS             58848..59024
                     /locus_tag="ONT67_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR2354397.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="ONT67_000638"
     CDS             59051..59497
                     /locus_tag="ONT67_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCR2354398.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT67_000639"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="ONT67_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2354399.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="ONT67_000640"
     CDS             61736..62707
                     /locus_tag="ONT67_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR2354400.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="ONT67_000641"
     CDS             62731..64923
                     /locus_tag="ONT67_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCR2354401.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT67_000642"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="ONT67_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR2354402.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65400..65795
                     /locus_tag="ONT67_000643"
     CDS             65400..65795
                     /locus_tag="ONT67_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCR2354403.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /gene="era"
                     /locus_tag="ONT67_000644"
     CDS             65810..66709
                     /gene="era"
                     /locus_tag="ONT67_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR2354404.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="ONT67_000645"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="ONT67_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR2354405.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT67_000646"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="ONT67_000646"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR2354406.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="ONT67_000647"
     CDS             68967..71042
                     /locus_tag="ONT67_000647"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR2354407.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DAJSBM010000008        63475 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJSBM010000008 DAJSBM010000000
VERSION     DAJSBM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.008"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(152..550)
                     /locus_tag="ONT67_000648"
     CDS             complement(152..550)
                     /locus_tag="ONT67_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR2354408.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="ONT67_000649"
     CDS             complement(547..1320)
                     /locus_tag="ONT67_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2354409.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="ONT67_000650"
     CDS             complement(1317..2000)
                     /locus_tag="ONT67_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCR2354410.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="ONT67_000651"
     CDS             complement(2167..3054)
                     /locus_tag="ONT67_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2354411.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="ONT67_000652"
     CDS             complement(3051..3920)
                     /locus_tag="ONT67_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354412.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="ONT67_000653"
     CDS             complement(4057..5343)
                     /locus_tag="ONT67_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2354413.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="ONT67_000654"
     CDS             5616..6194
                     /locus_tag="ONT67_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2354414.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="ONT67_000655"
     CDS             6194..7123
                     /locus_tag="ONT67_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354415.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="ONT67_000656"
     CDS             7120..8856
                     /locus_tag="ONT67_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2354416.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT67_000657"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="ONT67_000657"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCR2354417.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="ONT67_000658"
     CDS             complement(9752..10261)
                     /locus_tag="ONT67_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCR2354418.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT67_000659"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="ONT67_000659"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR2354419.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="ONT67_000660"
     CDS             complement(11562..12653)
                     /locus_tag="ONT67_000660"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCR2354420.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT67_000661"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="ONT67_000661"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR2354421.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT67_000662"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="ONT67_000662"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR2354422.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT67_000663"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="ONT67_000663"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCR2354423.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="ONT67_000664"
     CDS             complement(16616..17590)
                     /locus_tag="ONT67_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCR2354424.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="ONT67_000665"
     CDS             complement(17571..19454)
                     /locus_tag="ONT67_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCR2354425.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="ONT67_000666"
     CDS             complement(19472..21697)
                     /locus_tag="ONT67_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2354426.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="ONT67_000667"
     CDS             21812..22846
                     /locus_tag="ONT67_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2354427.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="ONT67_000668"
     CDS             complement(22913..23926)
                     /locus_tag="ONT67_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2354428.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="ONT67_000669"
     CDS             complement(24004..24192)
                     /locus_tag="ONT67_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR2354429.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24632..24760)
                     /locus_tag="ONT67_000670"
     CDS             complement(24632..24760)
                     /locus_tag="ONT67_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2354430.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT67_000671"
     CDS             complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="ONT67_000671"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2354431.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26247..27302)
                     /locus_tag="ONT67_000672"
     CDS             complement(26247..27302)
                     /locus_tag="ONT67_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2354432.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT67_000673"
     CDS             complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="ONT67_000673"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCR2354433.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT67_000674"
     CDS             complement(28739..30211)
                     /gene="gatA"
                     /locus_tag="ONT67_000674"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCR2354434.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT67_000675"
     CDS             complement(30211..30516)
                     /gene="gatC"
                     /locus_tag="ONT67_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCR2354435.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT67_000676"
     CDS             complement(30622..32658)
                     /gene="ligA"
                     /locus_tag="ONT67_000676"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR2354436.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT67_000677"
     CDS             complement(32675..34909)
                     /gene="pcrA"
                     /locus_tag="ONT67_000677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCR2354437.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            35064..36623
                     /locus_tag="ONT67_000678"
     CDS             35064..36623
                     /locus_tag="ONT67_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCR2354438.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(36734..38665)
                     /locus_tag="ONT67_000679"
     CDS             complement(36734..38665)
                     /locus_tag="ONT67_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2354439.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT67_000680"
     CDS             complement(38683..39597)
                     /gene="pfkB"
                     /locus_tag="ONT67_000680"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2354440.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(39594..40346)
                     /locus_tag="ONT67_000681"
     CDS             complement(39594..40346)
                     /locus_tag="ONT67_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2354441.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(40505..40714)
                     /locus_tag="ONT67_000682"
     CDS             complement(40505..40714)
                     /locus_tag="ONT67_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354442.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(40725..42053)
                     /locus_tag="ONT67_000683"
     CDS             complement(40725..42053)
                     /locus_tag="ONT67_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354443.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42189..42344
                     /locus_tag="ONT67_000684"
     CDS             42189..42344
                     /locus_tag="ONT67_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354444.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(42418..43005)
                     /locus_tag="ONT67_000685"
     CDS             complement(42418..43005)
                     /locus_tag="ONT67_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR2354445.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT67_000686"
     CDS             complement(43088..44338)
                     /gene="clpX"
                     /locus_tag="ONT67_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCR2354446.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(44585..45874)
                     /locus_tag="ONT67_000687"
     CDS             complement(44585..45874)
                     /locus_tag="ONT67_000687"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR2354447.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(46028..46960)
                     /locus_tag="ONT67_000688"
     CDS             complement(46028..46960)
                     /locus_tag="ONT67_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354448.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(47117..47776)
                     /locus_tag="ONT67_000689"
     CDS             complement(47117..47776)
                     /locus_tag="ONT67_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2354449.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT67_000690"
     CDS             complement(47779..48804)
                     /gene="sppA"
                     /locus_tag="ONT67_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2354450.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(48817..49107)
                     /locus_tag="ONT67_000691"
     CDS             complement(48817..49107)
                     /locus_tag="ONT67_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354451.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            49516..51321
                     /gene="glmS"
                     /locus_tag="ONT67_000692"
     CDS             49516..51321
                     /gene="glmS"
                     /locus_tag="ONT67_000692"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR2354452.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(51393..52748)
                     /locus_tag="ONT67_000693"
     CDS             complement(51393..52748)
                     /locus_tag="ONT67_000693"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR2354453.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(52772..53941)
                     /locus_tag="ONT67_000694"
     CDS             complement(52772..53941)
                     /locus_tag="ONT67_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354454.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(53938..54825)
                     /locus_tag="ONT67_000695"
     CDS             complement(53938..54825)
                     /locus_tag="ONT67_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCR2354455.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT67_000696"
     CDS             complement(55014..58706)
                     /gene="nifJ"
                     /locus_tag="ONT67_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR2354456.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            58905..59426
                     /locus_tag="ONT67_000697"
     CDS             58905..59426
                     /locus_tag="ONT67_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2354457.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(60125..60682)
                     /locus_tag="ONT67_000698"
     CDS             complement(60125..60682)
                     /locus_tag="ONT67_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354458.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(60861..61472)
                     /locus_tag="ONT67_000699"
     CDS             complement(60861..61472)
                     /locus_tag="ONT67_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354459.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(61585..61842)
                     /locus_tag="ONT67_000700"
     CDS             complement(61585..61842)
                     /locus_tag="ONT67_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354460.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(62439..62717)
                     /locus_tag="ONT67_000701"
     CDS             complement(62439..62717)
                     /locus_tag="ONT67_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354461.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(62751..63002)
                     /locus_tag="ONT67_000702"
     CDS             complement(62751..63002)
                     /locus_tag="ONT67_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354462.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        17688 a        13738 c        11220 g        20829 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattgat tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24601 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24661 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24721 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24781 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24841 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24901 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24961 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    25021 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25081 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25141 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25201 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25261 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg
    25321 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25381 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25441 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25501 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25561 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25621 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25681 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25741 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25801 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25861 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25921 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25981 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    26041 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26101 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26161 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26221 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26281 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26341 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26401 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26461 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26521 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26581 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26641 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26701 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26761 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26821 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26881 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26941 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    27001 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27061 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27121 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27181 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27241 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27301 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27361 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27421 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27481 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27541 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27601 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27661 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27721 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27781 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27841 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27901 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27961 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    28021 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28081 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28141 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28201 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28261 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28321 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28381 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28441 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28501 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28561 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28621 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28681 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28741 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28801 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28861 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28921 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28981 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    29041 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29101 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29161 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29221 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29281 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29341 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29401 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29461 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29521 cgctgatagc tgtcaatgct aatgcattat ctttcaccgt acgggtcatt ggtccgattt
    29581 ggtcaagaga tgaagcaaag gcaatcaaac caaaacgtga gacacggcca taagtaggtt
    29641 tcatccctac gatgccgtta aaagctgctg gttggcggat acttccccca gtatcactac
    29701 ctaaagaaac tggaatttgt ccagctgcaa ctgctgctgc agaaccacca gaagagccac
    29761 caggtacttt tgttgggtcc caagcatttt ttgttttctt gaaataagaa gtttctgtac
    29821 ttccacccat agcaaattca tccatgttta gcttaccaac agggatcata tctgcttgat
    29881 agattttatc catgaccgtt gcatcataaa ttggttcaaa attgtaaagg atcttagatg
    29941 cagcggttgt caagatatct tttgttacga tattatcctt gataccaatt ggaataccag
    30001 caaggacgtt gctttcatca atcccttttt catctaaagc ttttgcctgt tccaatgctt
    30061 tttcttcgtt caatgtaatg aatgcctcga cctcaggctc tgtttctttg attcgttcaa
    30121 atgttgtttt tgtcaaatct tgtacagtga tttctttcga tacaagcata tgatgcagtt
    30181 cttctagaga acgattgtgt aattctgtca ttatgcgcca gcctccccat tatcgatgat
    30241 tgcgggtact ttgataaagc cgtcttctgt ttccggcaca tttttcaata attcatcgcg
    30301 gttccatcca gcttgcgccc gatcttgtcg catcacgttt actgtttcaa ccacattcga
    30361 cgtaaatgga acaccttccg tatctacttc ttccagcatt tcaaccatat cgatgatttt
    30421 acctaattgg tcggtaaaac ttgcaagttc ttcatctgcg aacgcaagtt ttgctaattt
    30481 ggcaacgtgt ttcacttgtt cttcactaat cgccatacta ccccttcttt ctaaacgagt
    30541 taaaaacttt gctctctatt ttaacacaag agtgccgtgt ctgaaattgt ttattgaaat
    30601 ttgtctgaaa aaactcattt ttcattcttc tactgagcga cttttagcca atgcatctgc
    30661 catttgatct tctgtccaaa cttcgattcc taattcttgt gctttggtca gcttgctgcc
    30721 agcatcttct cctgctacca cgatatccgt ttttttcgat acgctaccag tcactttccc
    30781 gccaagattt tcgatcgtct cttttgcttc ttcacgagta aatcgtgtta gtttaccagt
    30841 aaggactact gttttttctt taaaaggaga ctcgacttct tgcaattggg catttcgaag
    30901 ccctttgtat tcgaaattga cgcctgcttg ttttaattca ttcatcaatt catggacttc
    30961 ttcgttactg aaataagtga ctacactgtc ggcaatgata tcaccgatcg tatcaagcgc
    31021 aataatcgac tcatagtcac ttttactcaa tgtttctaga tcaccgaaat gttctgctaa
    31081 aatttttgct gctttcgctc cgacatgtcg gattcctaac ccgaaaatca accgctcgac
    31141 cgaattatct ttgctgttgt cgattgctgt caaaatatta tttgctgatt tttcttttat
    31201 tttgtccaac gtcaataact cttcttctgt caatttgtaa agatcagcaa catctgctac
    31261 tagtttttta tcatacatct gttctagtac gcgcggcccc aacccatcga tattcatcgc
    31321 atttcttgaa acaaagtggt tcaaaccttc tttcatttga gcagggcatt taggattgat
    31381 gcaccgtaag gctacttctt catccaaatg gacaagttca cttccacaaa ctggacaatg
    31441 tgtaggcaat tggtattctt cgctgtcttt aggtcgttta tctaacacga cttgcgaaac
    31501 ttctgggatg atgtctcccg ctttataaat cagaactgta tctttcaagc ggatatcctt
    31561 caacttaata taatcaccgt tatgcaggct ggcacggctt actgtcgttc ctgctactcg
    31621 gactggtgtc atgatagctg ttggcgttac tacccctgtt cgaccgactg tccattcgat
    31681 gttttcgatt agcgtttcaa cttcttctgg cggaaacttg taagctgctg cccatctcgg
    31741 tgctttgata gtaaaaccaa gttgatcttg tagcgagaat tcattcactt tgatgacgat
    31801 tccatcgatt tcatagggca aatccacacg tttgtcatga tattcttcaa tgtatgacca
    31861 cacttcatca atcgaatgac atagacgttt ttcccgattc gtgtggaatc caattttttc
    31921 tagctcttct agcgcatcat actgggtttt tgcttgcata ggaccgaaat cagctactgt
    31981 gtataaaaaa gtgtccagat ttcttttcgc tgtgatttta gaatctaact gacgcaaaga
    32041 accagctgcg gcgttccgag gattcgcaaa gatatctttt ccctctgctt ctctgtcttg
    32101 gttcaattgg acaaatgacc gttttggcat gaaacattcg cctcttactt cgatgttcat
    32161 tggttctttt aatttgattg ggaccgaccg gacagttttt aggttttcag tgatgttttc
    32221 tcctacagtt ccatccccgc gagtcgcacc tctcacgaaa tttccatctt cataacgcaa
    32281 agaaacggaa agtccatcta tttttagttc acagcaatag tcaactacac gtcccactgc
    32341 tttttgtaca cgttgatcaa aagctatcag ctcttctttg ctgaaaacgt cgttcaaact
    32401 gtaaagcgga atatcatgag tgactttttc aaaaccttct aatacttttc cgcctactcg
    32461 ttgagtggga gaatcagacg tgatcaaatc aggatattgt tcctcgattg ctactaattc
    32521 tgcgtattct ttatcataaa catagtcttc cactgttggt ttatcttcta cgtagtattc
    32581 gcgagaccat tggttcagtc ttgtacgaag ttcttctgct cgtttagcag cttcgtcaat
    32641 cgtcatttca tgttccatca tttaccctcc actactaagc tttttcaatt ggcgcaaagg
    32701 cagccaaaag acgttttact ccttgacttg ggaaagccac atctagttct agatccttcg
    32761 acgtcccgct tactcggacg actgttccta gaccccattt tttgtgtttg actttatctc
    32821 caggtttcca gctgttattt tcccctccag tagcactttt agagggaaca accgctgtct
    32881 ctggctgttt atagctatat gctgtttttt tagcgaatac aggcgttttt ttaggcgtag
    32941 gacgcatgcc ttcgatttcc agtaactcct gatcgatttc ttctacaaaa cgagaaggtc
    33001 ggttatattg cgtccgtcca tataatgttc tggagaaagc atttgttaaa tacaacgctt
    33061 cttctgctcg cgtgatcccc acatatgcga gacggcgttc ttcctccagt tcgctttctt
    33121 ccatcaatgc tcgagataaa ggaaaaaccc cctcttctag accaatcagg aatacaactg
    33181 gaaattctaa tcctttggct gcatgcaaag tcatcaatgt cacttgggaa gcatcttctt
    33241 ctaaattgtc aatgtcggaa acaagtgcca gatcatttaa gaagaccgtt agtttttctt
    33301 ctggtgcatc agcgtcttct tcattttgct gttcgaattg tttgtcgaat tcttgtgtga
    33361 ccgttaaaaa ttcctccaag ttttcgatgc gtgcttgtgc ttctaatgta ttttggattt
    33421 ttaagtcttc tagatatccg ctacgatcca aaacctcttt cgtcaattct gtcactgtca
    33481 agtatgggat catctgagtg acttcttgga tcatctctcc aaatgcacct aattgctgtc
    33541 ctgcttttcc accgatattt gccagatcca cattttgcgc tgcttcaagc agtggccatt
    33601 catgaagaga agcaaaactg cgtagttttt cgatagatcc tggtccgatg cctcgtttgg
    33661 gcgaattaac gatacgctca aaactgattg aatcttgagg atttaccagc acattcaaat
    33721 acgctaaaat atctttgatt tctttacgat cgtagaactt gtgccctcca accattttat
    33781 aaggaatatt tgctttcaac agagtttctt ccattacacg agattgtgcg ttcgtccggt
    33841 acaagatggc aaagtcgccg taattccgat gattgcttcg aatctcttct tgcatccggt
    33901 ctacgataaa acgggtttcg tctcgttcat tatctgcacg gtagtaagtg atcttgtttc
    33961 cttctttgtt ttctgtccac aagttttttg gttttcgatt gctattgttt tcaatgactt
    34021 ggtttgctgc gctaaggatg tttttcgtgg agcgatagtt ttgttctagc aaaattactg
    34081 cagcatctgg ataatctttt tcaaaatcca agatgttctg catatctgca ccgcgccagc
    34141 cgtaaatact ttgatctgca tctcccacga cgcacaggtt cctaaatcta cctgccaata
    34201 aattgactag cgtatactgt gcgtgatttg tatcttggta ctcatctaca tgaatataat
    34261 ggaatttgtt ttgataatac gtgagtgaat caggatgttc ttcaaataat cgaatcgtat
    34321 tcatgatcag gtcgtcaaag tccatacatt gattatattg aagctctttt tggtaagcag
    34381 cataacattt tgccgcaatc tcttcgtaat aactgccttg catttttgca aaatcctgag
    34441 gggtctgcag gctgtttttt gcttgagaga ttgttcctaa tatacttcga ggatcatatt
    34501 ttttcggatc aatgttcagt tcttttagta tccgtttcat caaggtcagc tgttcagaag
    34561 aatcgatgat tgtaaagttc cggttatacc cgataaaatc aacatctctt cttaaaatac
    34621 gcacacacat cgagtgaaaa gtcgataccc agacatcttc gcctccagat gcgagaatcg
    34681 cattgacacg ttctttcatt tcttttgcag ccttattggt aaaagtaatc gctaggatgt
    34741 tccaaggatt gacttctttt tcctcaatca aataggcaat gcggtgtgtc agcactctcg
    34801 tctttccact tcctgcacct gccattagca gtaatggtcc gtcagtgtga agaacagctt
    34861 ctttctgacg ggggttcatc ccgtttaata attcagcttt ggatgacatt aacaacatcc
    34921 tttcttttat caatcttgtt aattatagca ttttcttggc tttcattaaa ctagctagtt
    34981 caaaatcatt tgtttctttt tatggatttt tccttgtcaa ttcgttgatt tcgcgctaaa
    35041 ttaaaagaaa gcgaggcgtt tgaatggaaa aaaaaggaaa aatcaagaaa ttagataaga
    35101 ctcagatata ttttattcta aaaggaatat ttgttggact gctgacagga atcgtcgtta
    35161 gcctgtttcg attatctatc gaaaagctaa gcgatttagt tcgaagtatc tatgaaatga
    35221 gtcgggaaca gccgatttac ttaattggcc tagctatttg ctgcatcctt gcagcatttt
    35281 tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg aagcgggatt ccacaagtag
    35341 agggacagct tcgcggggaa ttatcaatga actggttctc tgttttatgg aaaaagttca
    35401 ttggcggtgt actttcagtt ggtgcgggcc tttttctagg tagagaagga ccttctatac
    35461 agcttggcgc atccattgga caaggagctg gacaattgtt tcggagtcca tcttcagaag
    35521 aaaagatttt aatttctagc ggtgcaagta gcggacttgc agcagcattc aatgctccta
    35581 tcgcaggatt actctttgtt ttagaagaaa tccatcactc cttttctcca cttgtttggc
    35641 tgacatcatt tgcttcagca atcactgcta acttcgtctc tctttacttt ttcggcttga
    35701 gacctgtttt atatctaggt gaccttcctt ctttgccgtt gaaatattat ggttccttag
    35761 tgatcttagg agtacttcta ggcatcttag gttttattta tcagaatgtc ttgctggctt
    35821 tgccaaaatt ttacaaccga ttgcctttac cagcttattt tcacgggttc gttcctttct
    35881 tgctgatttt gccaattggt tatttatggc ctagtactct aggcggaggc aatcaaatcg
    35941 ttttagcttt tggacatgct tccttgccaa tcattatgat tatcggtttg tttgttcttc
    36001 gattcgtttt ttccatgatt tcttacggtg ggggtcttcc aggggggatt ttccttccga
    36061 tattgacatt gggggcttta atcggtagct tttatggaaa cattatggtt gatttcggga
    36121 tggatccaat ctacatcaag gattttgtcg ttattgccat ggctggttat ttcacagcga
    36181 ttggaaaagc accgctgaca gcaattattt tagtgacaga aatggttggc tctcttaatc
    36241 atctgatgcc tttaggattg gctgctcttg ttgcttatat cgtcaatgac cttctcggcg
    36301 gtaatccaat ttacgaatca ttattggaac gtctacttgg aaatactcag accgcctcac
    36361 taaaaggtgc aaggacaact tttgattttc cagtaacggc agagagttct ttagacggat
    36421 taatggtacg agacttcaat tggcctaaaa atatgctgct aatctctata cgcagaggaa
    36481 atcaggaaat cttgactcac ggtgatacga tcatgaatgt aggagatatt ttggttatct
    36541 tgactgatga agcaaacttg ccaagaatca agcaggaaat cgaggctaaa tcttctatca
    36601 caaatttgaa gcaatcattt taacgaacat ggaaaaacta ttttacaaca actattcatc
    36661 tacactactt gattctatat gattctataa aaaaagaaga ggctgggaaa acccagcctc
    36721 ttctttttta tttctatgct tctgctactg caggagcttt tctcaataaa ccataaatca
    36781 caccagatac gactgctccg atagcaataa acaccaaata catgaccggg tgactcagta
    36841 agaatacaac gaaaatcccg ccgtgaggag ccagtaattt gattccgaaa gctccgacta
    36901 atccgccagt taaagcagaa ccaataataa aacttggaat ggcacgaatc gggtcagctg
    36961 ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc catcacgatg ttcgtcaaac
    37021 cagcatcttg atctgttttg ctgaatttat tcttaaacaa acgtgtagca acgaaaattg
    37081 ccaatggagg taccattcct ccagccataa cagaagccat cacgacactt ccgccagttg
    37141 caacagtcgt agctaaagtt gctgtaccaa acacataagc ggctttgttg attggaccgc
    37201 ctaaatctgc cgccatcatt ccagctaata atgcaccaag taaagcagca ttcgaaccac
    37261 tcatacttgt caagaaaccg ttcaatgcat cattgattgc tttcataggg acattgacca
    37321 gcaacatcaa gaaaccagta atcagtaagc caaacactgg ataaaagagg atcgttttaa
    37381 ttccatccaa tgattttggt aagttcttga ataatttacg taaaaatacg atgacataac
    37441 cagctaagaa tccaccaatc agtgcaccta agaatcctgc accgcctgta ttagctaatg
    37501 caccagcagc aaatccaacg atcaaaccag ggcggtcacc gatacttgaa gcaataaatc
    37561 ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc gatttggttg aaccagctag
    37621 cagcttgatt gtaattccct agatttgata attgatcatg tggcacgcca ataaattggt
    37681 cgatcatgaa agaaagcgca atagcgattc ctccaccaat aacgaaaggt aacatgtgtg
    37741 atacgccgtt catcaaatct ttgtaaattc tttgtccgat agtaccatcc gcatttcctt
    37801 cttctgaact gctttctttt ccatcaccat ggaatactgg tgcagtacca ctaagggcta
    37861 gattgatcaa ctcttctggt ttacggatac catcactgac aggacgattg atcagttctt
    37921 ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc aacgattaca ccttctgcac
    37981 gggcgatgtc ttcagcagtc aatcggtttt tgattccttc tgaaccgttc gtttcgactt
    38041 tgatctccac acccatttct ttcgcttttt tcttcaaagc atcttctgcc atgtaagtgt
    38101 gagcgatccc tgttggacaa gctgtaacgg caacaacgaa ttttctttct ggatgagcag
    38161 aagcttctgc agcttgtgtt tcttgttctg ctttttcttc tgcttctttt tgctcttcag
    38221 ctgtttggaa caagtcttgg acttcttctg gtgtatttgc ttcttttaat tttcctacga
    38281 aatctgcatc aatcagcaat cgagatagtg ctgctaatgc ttgcaaatgt gtgtcgtttg
    38341 cgccttctgg agctgcgatc atgaaaaaca gataagttgg ctgtccgtct aaggcttcat
    38401 aatctactcc tttttggctt tttgcgaata aaactgttgc ttctttaact gcactgtttt
    38461 tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga agtttgtgct tcgcgggcta
    38521 gaattccttc tttatatgtt tcgatatcgg aaatccggcc ggcatcaaac attttttgga
    38581 ccatttcatc gattgcgcct tttttatcag tcgcttgcaa atccatgatc atcgcttctt
    38641 tgaccattaa atccttgata ttcatgatta tcctccttga aactaatcaa tttttgtgat
    38701 ttcaacttct ggcagcaact catcaatcaa ttcgcgagct gccaagtcat cagaaaaagc
    38761 tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg attggatctt tcgtacggct
    38821 gtaagcccca ataaagccag caatcattga atcccctgca cctactgaat ttttcaatgg
    38881 gcgaactaat acattcgttt tataagttcc ttgttccgtg aagagcaaag caccgtctcc
    38941 agccattgag atcagcacat tctgtgcccc ttcagctagt aattttttcc catacggaat
    39001 gatttcttct acagattgga aggttgtgtt gtaaagctca gctaattcgt gattgtttgg
    39061 tttgatcaac aacggtttct taacaagtgc attttttagg tcatcacctg ttgtatcaat
    39121 cacgaaatct gcaccttttt ctcgaatgat atcaatcagt tcttgataaa atccttttcg
    39181 caggctagct ggcgcgcttc ctgacaatac gacgatatct ccttgtccta ctgttcctaa
    39241 tgcttgcttc aactcatcga tttcttcatc tgaaatagat gggcctaagc cattgatctc
    39301 agtttcagtg tctgatttta gtttgatatt gattcttgta tcttctttta cggttgtgaa
    39361 caaagactgg atttgttctt tttcaagcca cttcgaaata aattcgccag tgaaccctcc
    39421 taaaaaccct agtgctgtag attgagaatc gatccgtttc aaaatacggg aaacattgat
    39481 cccttttcct ccaggtaact tgaagtcttc cttcattcgg ttcaaatcgc ctagttttaa
    39541 cccatctacg cgtacgatat aatcaattga tggattaagt gttactgtgt aaatcattgt
    39601 attgcctcct taattgttgt cttttcacgg aattcattaa cgaattctaa tggacaacgc
    39661 tcggttatga tcgtggctgc ttccaaaggt gcaacagagg tgaaggacaa ctggttgaat
    39721 ttggtatgat caatcaatac aaatgtctct tcggaatttt tcatggctaa acgtttaaca
    39781 gctgcctctt ctggatccgg agtcgtgaat ccttgttcaa gatgggcacc gttcattccc
    39841 ataaatgctt tattaaaatg aaaatttttt agttgttcta tcgcattaga tcctaaaaca
    39901 gcattagttg ccagctttat cgttccacct aaaataatgg tagcaagctc caaatcaact
    39961 aaatttgccg catgttgaac ggaatttgtc actactgtga tctgttttcc ttgcagaaaa
    40021 ggaatcattt ctaacgtagt agagccagcg tctaaataaa tgacatcttc gtcattaatg
    40081 gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa cgtttttgag tgatttttca
    40141 ctcatattct gctcaaaatt caataattgc tctttcttag ctccgccatg aactctttct
    40201 agaaacccac tattctccag ctcttgtaaa tcacgtctta tcgttgattc agaagcattc
    40261 aaaaggttta caagatcttg tgatttcacg attttctgct gttcaagcaa gtctaggatt
    40321 ttttgtctgc gttcctctgt aagcattttc acgatcacct caatacaaat aatagcatat
    40381 tttttttcta ctagcaatat aaatcagtca aaaacgttca taaaaattat aaatcagtca
    40441 aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct atcatcaagc
    40501 cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa gcaggttttc
    40561 tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc aaaaaaccga
    40621 tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat aatgatgccg
    40681 gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat tttttgtaaa
    40741 gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg ctgctcacct
    40801 ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa ggtaacatca
    40861 gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga aggaacatag
    40921 attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac ttctagatat
    40981 tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt cggatcgata
    41041 actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata cgcctgagca
    41101 tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc ataacttttt
    41161 gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt ctgatactgg
    41221 acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt caataaaccg
    41281 acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata ggattgaaac
    41341 atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat acatatgact
    41401 tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat cggtttaggg
    41461 atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa acataaacct
    41521 tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc cccgttttgt
    41581 tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag ccgttgagag
    41641 atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc gaacacccca
    41701 tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc tttgaaaaca
    41761 gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa actacattcg
    41821 ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag cattattttt
    41881 tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg gataaatgtt
    41941 ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt tttttcttct
    42001 tcatttaagt ctgtgagctg gatagtcatc aataatttat cttcgccaat cattttctca
    42061 cctctttcaa atctttttat aatttaatta taaccaatga taaagaaaaa ctaaaacttt
    42121 atgtcttaga aaccaatttg gacgattaca tgatagacta agtaaaaaga aacaaagagg
    42181 tgtttacaat ggggttgaaa tttgaacgta cagataattt agataaactt ttcgaatcat
    42241 ttgctgtcga tccggaaaaa acagttgcac caaaagaaga gatcgatcga tttctaaaac
    42301 cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa gtaagttaag ctcaaaaaaa
    42361 gacttaccag ccaaactttt atgaaagagt ttgactggta agtctttttt attcttttta
    42421 tgattcaatt gcttgttcga ttgctgccca cgcttcttct tttccctgtt ttgtaacgga
    42481 agaaaagaga ataaacgtgt cattgcgatc aaaatccaac gcttttttga tttcgctctc
    42541 atgcttgttc catttaccac gagggatctt atctgcctta gtggcaacaa caattacagg
    42601 gatatcataa tacttcaaaa attgatacat ctggatatct tcttgagaag gcttatgacg
    42661 caaatctact aaagaaacga ccgcacgtag ctggtctcta gtcgttaaat atgtctcgat
    42721 catctttccc cattttgctc gctcagtttt ggatactttt gcatacccat atcccggaac
    42781 atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc aatgtctggg tctttcctgg
    42841 cttaccggaa gtacgggcta aattctttcg atcaatcaat gtgttgataa aagaagattt
    42901 ccctacattt gatcgtcctg ccagagctat ttctggcaat tgtgtatctg gatattgttt
    42961 tggtgccact gcacttatga taatttcagc ttgatggact ttcatggtct ctctccttaa
    43021 caaggtgatg aataaagcaa gcggctgtcg caaaagccaa aatgactttt acggcagcct
    43081 cttttcatca acccgctttt ttctctgaat ctttatagat tactgttggc tcaccagaca
    43141 attcagctgc ttcttttgtg ataatcactt tttcgatgtt ttcatttgaa gggatatcaa
    43201 acatcacatc catcatgatt tcttcaatga tggaacgaag tcctcgagca ccggtattgc
    43261 gttcgattgc ttttttcgca atggctctca acgcttcagg ttcgaattca agttcggtat
    43321 tatctagaga aagaagtttt tgatattgtt tgactagcgc atttttcggc tcagtcaaga
    43381 tccgtaccag atcatctgtt gtcaatttct ctaaagctgc cataacaggt aatcgtccga
    43441 taaactcagg gatcaatccg aattttagta aatcttcagg aatgatatgc tgcatgacac
    43501 tttcgtcttc agaaagtttt tggttttgtg taccaaatcc aatcgtcttt tcgcccatac
    43561 gattcttcac aatcgtttcg atgccatcaa atgcaccacc tacgatgaac aatatgttcg
    43621 tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg tccgccctga ggtggcacac
    43681 ttgcaacagt tccttctaga attttcagca aggcttgttg gacaccttcg ccggaaacgt
    43741 cacgagtaat agatacattt tcgcttttac gcgcaatttt atcgatctca tcaatataga
    43801 tgatcccttt ttctgcaagt tccacattgt aatcagcaga ttgaagcaat ttaagcaaaa
    43861 tgttttctac atcttccccc acataaccag cttctgtcaa acttgtcgca tctgcgattg
    43921 caaaaggtac attcaatgtt tttgctaatg tttgtgctaa aaatgtttta ccagaccctg
    43981 taggtccgat caagcagata ttgctttttt gcagttcaac atcatctgtg ttttcttgtg
    44041 cttgctgatt gacacgttta tagtgattgt atacggcaac agctaacgag cgttttgcgc
    44101 gatcttgtcc aatcacgtag ttattaaata cttcaagaat ttctagtggt ttagggactt
    44161 cagtgaattc gcgtacagct tcttcataaa attcttcatc aatgatttct ttacataggt
    44221 cgatacattc attacagatg tagacccctg gaccagctac gatctttttc acttcttctt
    44281 gggttttccc gcaaaaagaa cagcgtacgg tctcattgct acttggattg tcatacatgg
    44341 tcttcacccc ttaacttgca aaaagtatgc aagaaactat caaagacttt acagtctttt
    44401 actatcataa catagtttag taaaaagaaa aagtgtttaa caaaggatca actgacctat
    44461 acctttgcat tttttactct taacaaaaaa taaggaactg tgacaaaatc gtgtcacagt
    44521 cccttatttc gaataaacaa ttttctagaa gtagcttctt cagaaatgat ttattacttg
    44581 atttctattc tttcgcttct gtcgctgtac ctgtgatcaa ttcgattgct tttttcattg
    44641 tcacatcatg ttccagcata tcttctgtca acacgcgttt gatttgttcg attggcatgt
    44701 tgtatgtttc tgataattct tggatttctg cattgatttc ttcttctgtt gcttgaagat
    44761 tttcagcagc tgcaattgct tcgatcacaa gatttgttct tgtgcgcatt tctgcttcac
    44821 cttcgaattg tttatgcaag tcttcttctg tagaaccagt taattgatag tacatttcag
    44881 gagagatacc ttgacgttgc atgttgttca agaattcgtc cattgaacga tgtacttcat
    44941 catgaaccat cacgtgtggc aattcaacga tttctgcgtt ttcaacagcc atacggatcg
    45001 ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt ttctactaat tctttgcgat
    45061 atttttcttt caattcgtct aatgttgata cttcgtcatc aacatctttt gcgaattcgt
    45121 catctaattc aggcacttct tttgctttta cttcatggat tgtcactttg aagactgctt
    45181 cttttccagc aagatcttct gcttgataat cttctgggaa agttacattt acatctagac
    45241 tttcacctga tttatgacca actaattgat cttcaaaacc tggaatgaat gagcctgaac
    45301 ctaattcaag tgagtaattt tcgccttttc cgccatcaaa cgcttcgcca tctacaaagc
    45361 cttcgaagtc gattactact gtatcgccat tttcagctgc agcatcttct ttgataacta
    45421 attcagcttg tgcttcttgt tcacgtttga tacgtgcatc cacgtcagca tctgttactt
    45481 cacggtcttg tttttcaaca actagatctt tatattgtcc taattttact tctggtttca
    45541 ctgttacttc agcagtgatt acccagtctt gacctttttc catgctttca acatcgattt
    45601 ttggttgaga aacaggatcg atgccagctt ctttgacagc agcttcataa gcttctggta
    45661 atacagcatt caatgcatct tcatataaag cttcttcacc gtacatacgg ttgaatactt
    45721 gacgagaaac tttcccttta cggaatccag gtacgttcaa gctttttttc actttattaa
    45781 acgcagttgt taatcctttt tggatttcgt cttgcgcaat cgtaaatttt aaaacaccat
    45841 cattcgtgcc ttttttttca aatgttgcag tcatttaatt ccctccgagt ttagaaattc
    45901 attttataca tcaaaaacgt ttcatgcata cctttaaata gtacactaac cctttcaaat
    45961 tgtaaacata ttgaatggat agaatccttg tttttcataa catttttatc tttttcactg
    46021 ataaaatcta atggaagaag atcatttgct gcaaaatttt ttctcttttt tttaaaaaat
    46081 cttgtttttc atcttctgta ccttcataca tcgtttttcc tgtacacgcc tctacagtca
    46141 gttgaaacca gcgcttcggg tcttcgatgt atgtatcata gacaggataa acaattgcag
    46201 attgcaacgt gaattcttct tttaacacgg aactaagcag cgcatcttca ttcccgtaac
    46261 gatctgaaaa tacacataat tgctggtaac ttgcctgctg ctgcggcttt ggcagatctt
    46321 ttggtatgac tttgactaac ttttcttcaa tcgtcaaata gaaaacagga tggcattcgt
    46381 tcaattgcct aagtgtctcc agtaattttg cccttgctaa aatatggacc tctggcgata
    46441 caataagttt actcgctgtt tggataaact catgataagg aagctgttcg atctcatcaa
    46501 tagacgcaag ctgacgtgca ggttccaacg aaggaagttc ctccgattta ttgtggagtg
    46561 ccttgatctt ctgcctctcc atttgtccgg ataattcttc tgcaaattgg attttttcaa
    46621 tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc tatcaatttt ctagcatata
    46681 aaaaatcttg tgtactgatt aacaagtcaa aataaaattc agcaaactcc tcattttgaa
    46741 gatactgttt ttcatgcaag agtgcttgct tcaatgcttc ttttttctcg tccaactcgt
    46801 aaagacaaaa aacaagcagg ctattagcct caaagctttc tttcaactcg taagctcgtt
    46861 gaaataattc ttttgcctgc tgatattgct ggtttgctag tgcttgttgt gccactcgca
    46921 ttaaattctc atatttttct ggaaaatcaa tcggcttcac gcagtgtcct cctcttgctc
    46981 atattcagct catgttcaat agtagcaaaa caaaaaaagc atgacaatta aaaataactg
    47041 aagcaccctc taaaagctag attttagatc tgacttttag aaggtgcttt caactgttac
    47101 tgctttttat tgctcatcag ctgttgactt cctggttgaa cgctttgatc agattgatcg
    47161 taacaacact tgtattagtg aaaaaatcgc ccagatgttg ctttttctga ttaaagactg
    47221 ctggaagata ccctaacatc agcaaaggaa attttagaat gaaccggcaa gcaccttctc
    47281 taactaaaac ggttgaccag ctaagttctt cttcatcaaa gctgatgact cgaataccaa
    47341 agatcatttt cccaatcgtt tggccatgat tgaatttcgt caataaaata aaatacgcta
    47401 gataaataag taaggctgtg aatccgtaga gactcagata agaatctgaa aaattaagcc
    47461 aacctagatt tttggcaata ccaagtgtta aacgcgtaat agctccgata cacaaaagat
    47521 cgaccaagaa ggcaaacatt cggataaaaa aacctgcata gaaatactta ggaaaatcat
    47581 ttgcttttgg cggttcattt cttgtgtccg tatacttttt ccatttacgt tgtttctttt
    47641 tgatttcttc gggcgtcaaa ggctctcttt caaaagagct cagtaccttt tgggaaaagt
    47701 tggagtcagg tcctacagca ggctgctgtt gcttatcttt ttcttgttcc ttattttcat
    47761 ttgaaagctc tgacatgctt actcacctcc gtaataatac attgcttttg gtgattccgc
    47821 tgtccctaga ttctcaagaa cggataagat ctgactagtc tcagaaggct tcaatccctg
    47881 gaattctgct agtttacttc ctaaccatgt actagcaaac ccagatggcg tggaagaata
    47941 ttctactagt tccgcatctt caagctgttg ttcctttcgt atagctttta aagcatcttc
    48001 aggaaatccc aattcatcga ctaaacctac ttcttttgct tggacaccat cataaatcct
    48061 tccatcagca atttttttga cttcttcttc agatttattc cgtccctcgc tgacgattgc
    48121 tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc accgcttctt cttcttttgt
    48181 ctctggtcgc gttcctgaac ccatatcttt caacgctccg cttttaactg ttgtatcttc
    48241 cacacccagt ttttctagaa gtcctgaata attcaatcct gacatgatca caccaattga
    48301 gccggtcact gtttcttctg ttgcaaagat tttatctgct tgagcagaaa tatagtagcc
    48361 accgctagct gccatgtttt tcatgctgac atacattggg atatcatgtt ctttacgaat
    48421 cgtatccaat ttcttagcga tttccgcact ttcgtagaca ccgccaccag gagaatttac
    48481 ttctaaaaag atacctttga ctgttggatc ttcttcaatc gccttcagtt gttccataaa
    48541 atttttatga ttgtaacctt cagaagcaaa taaacctgaa gaccctcccg cagcaatcgt
    48601 tccatctact gataatttta cgattttttg cgtactgtct ccctcttcca acacatttgg
    48661 agctagttct tcatccccat ataaccaact gttcaatccc tcaatttgat cgttttcctg
    48721 aggcttagtc aaactagctg aaactaacga aaccacaaat aatcctgcag caatcagaac
    48781 tgctgcccaa cgtcttttat tcattctttc tcctttctaa tacaagtttt ttatgacgat
    48841 cacagtttct gactgctcag aagtgtcgaa ccgctctgct ttttcttccc gcgcataccc
    48901 ggaaaacgac tcttttgtta attcatcttc taccgtaaac tcacgagctt gagcaacttc
    48961 caaggcatca ggtgcgattt cttggatgct atcccagcca gtatctaaaa atattttcgt
    49021 atcatctgat acaccagctg atgcttcgaa tttggacaat tgttttttta acattcccag
    49081 tgtcatgatc tcaatttttg aatccatttt tactcctcct ctatccgatt actttctatt
    49141 gtaccgaact cctgaaaaag atgatagata aaaacccatt attttttgac ataaaaagga
    49201 catttgacaa agaatttgat atataccaat ttatttttta ttcttttttt gttgacaaga
    49261 acattcgcaa aaggttatga ttttaaatgt ttccagataa aaaacaaaaa ggaggatagt
    49321 tatacctttt aggatagcgc caggtctgtg taaacagatg acgaggagag ggtttatcga
    49381 aagattcggc ggatggccct agggactgca ctctacagaa aatccacaaa aaatgtctgc
    49441 aaagataaaa caaaaggatt ttcctgcagc tggaagcatt catacgaatt cattatagaa
    49501 agtaggatgt tttttatgtg cggaattgtt ggaatgattg gattaaaaaa tgtaacacca
    49561 ggattgattg acggtctaga aaaattagaa taccgtggtt atgattcagc tggtatcttt
    49621 gtctcagatg gcacaactga ttatctggtc aaagcacaag gcagaattca aaatttgaaa
    49681 aacaaaataa atattgatac tacaggaaat ataggtattg gtcatacacg atgggcaact
    49741 cacgggcaac cttctgaaga aaacgcccat ccacatactt cccaaagtgg acgcttcgtg
    49801 ctcgttcaca acggcgtaat cgaaaacttt gaagaactta aaattgctta tttagcaaat
    49861 gaccacttta ttggtgagac cgatactgag attatcgctc atctaattga aactttcgcg
    49921 aaggatacta caacgcaaga ggcgttttta aaagctttgc gggtcatcaa aggttcttac
    49981 gctttcgctt tgattgatcg gacagcacct gatgttattt acgtcgctaa aaataaaagt
    50041 ccactattaa tcggactcgg cgatggattc aatgtgattg ccagcgatgc aatggctatg
    50101 cttgctcata cgaaggaatt cgttgagatt gaagatgaag agatggttac cgtcacatca
    50161 gaaaaagtta ttattcaaaa ttttgcaggt gacatcgtag aacgttcttc attcgaagca
    50221 caagtagatg cttcagatat cgagaaaggt acctatcctt tttatatgct gaaagaaatc
    50281 gacgaacagc ctattatcat gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt
    50341 gtctctcttg atcaaaagct ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt
    50401 gcttgcggaa ctagttacca tgcggggtta gcaggtaaac agactttgga aaaattgaca
    50461 cagattcctg ttgaggttca tttagccagt gaatttggct ataacacacc tcttctttca
    50521 aaaaaaccat tctttatttt cttgagtcaa agtggcgaaa cagcagacag ccgccaagtg
    50581 ctggtaaaaa tcaaccgtct cggctaccct tcacttacga ttacaaatgt tgctggttct
    50641 actctttcgc gggaagcatc ctttacactg ctattgcatg ctggacccga aatcgcagta
    50701 gcatcaacga aagcttatac tgcacaaata gctgtactaa ctttattagc caaagctatc
    50761 ggtgataaaa aagaatttga aacttctctt gtattcgata tcactcatga attgagttta
    50821 gttgctaatg cgatggaatc ggtcatcgcc caaaaagatt atttagaaga gttggcagaa
    50881 gtatatctca gcgatacaag aaatgccttt tacattggac gtggtgctga ctacaacgtt
    50941 tctcttgaag ctgctttgaa attgaaagaa atcagttata tccaggcaga aggttttgcc
    51001 gctggcgaat tgaaacatgg aacgattgct ttgatcgaag aaggaacacc agtgatcgga
    51061 atcatcagtg aagaagtgac tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc
    51121 cgtggcgcta aaacactagt tattgtctca gagggattag aaaaggaaag tgaccagctg
    51181 gtgctaccaa ctgtccaccc ttatctaact actctaacca cagtcgtacc gactcaatta
    51241 cttgcatatt acgcaacgct attacgggga ttcgacgttg acaaaccacg taatttagcg
    51301 aaatcagtga cagtagaata ataaaagaac aaggaccagt gtcagacatc tgacactggt
    51361 ccttgttctt ttatttttta tgaaaatatc tcttaatcca gtccgatttc ttctttgact
    51421 actgccgcga ttttatcgac atagtaatta actttctcat cagtaggcgc ttctgccatc
    51481 actcgaagta atggttctgt accggaagga cgaactagga ttcttccttc tgatcccatt
    51541 tcagcttctg cctcttcaat cacttttttg attgccggaa catccatcgc accgttttta
    51601 tcacttacgc ggatattcac taatttttgc ggatagattg ttacctctgc tgcaagctct
    51661 gataatttct tacctgtctg tttcatgata ttcagtaact gaattcctga aagcatccca
    51721 tctccagttg tgttaaaatc taagaaaatc atgtgcccag actgctcgcc gccaaagtta
    51781 taatcgtttt tgcgcatttc ttctacaacg taacgatcac caacttgtgt aacgacatct
    51841 ttcaaaccga ttccttccac cgctttgtgg aatcctaagt tactcatgac agtcgtaacg
    51901 atcgtatctt ttttcaatcg atttttttct gccaagtatt tagcacagat aaacatgatt
    51961 ttatcaccgt cgacaatgtt tccaagttca tccaccgcaa taatacgatc gccatcgcca
    52021 tcgaaagcta gtccagcatc tgctcctttt tcaacgacca tctcagctaa acgttctgga
    52081 tgggtagagc ctacgccgtc attgatattt aacccgtctg gatttgtgcc cattgtataa
    52141 aaatctgttt caagatcggc aaacaaacga ttaacacttg ttgaagttgc tccgtttgct
    52201 gcatcgacac aaatggtcaa accggaaagg tcaccgttga tcgtttgtac aaggaattga
    52261 gaatatttca ataagccttc tggaaattct tctacagttc ccaatccttc agctgatgga
    52321 cgaggcaatt catccacttc agcatctaat aatgcttcaa tttctagctc ttggtcatct
    52381 actagtttaa aaccatctgc accgaaaaat ttgattccat tatcttgtgc tggattatga
    52441 gaagcagaaa tcatgacccc tgcgcttgct ttttgcaaac gtgtcagata agcaacacct
    52501 ggtgtagaga tcacacctag ctggaacact tcgataccca ccgataaaag tcctgaaatc
    52561 agcgcttctt ctaacatttg acctgagatt cttgtatccc gccctactaa cacgcgtggg
    52621 cgttttcctt cttcttcatg ctggcttagg acataacctc cgcaccgtcc cagcttaaat
    52681 gcaagttctg gcgtaagttc cttgttggct tcaccgcgta cgccgtctgt tccaaaatat
    52741 ttacccatag ttgtaaaatt actcctttaa cttaattttc tgaggaattt gttctgctca
    52801 tttgtgttga ttcgcttgtt tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc
    52861 cagattccga actggaagaa atggttgttg tcgatggttc cgttcctgaa tcagcagatg
    52921 aaatacttct actcgtactt gtattttctt gtgtccgact gctcgtcgaa gcagagctac
    52981 tgcttgacgt ttgcaacacg ggtgtaatct ccacactaac tgattcgggc ttgatactta
    53041 acccctcttc tactggtatt tcaattttac gagttgttcg atgattgata cctgagacat
    53101 tgatcggtac agcaatactg tcaccgatcg atgcaagcaa atcgctagag ccgcttactt
    53161 ctgcttccag ttttgaaaaa ctgaaatcat agctttctac cccggcagct ggtgttcctt
    53221 gctgtatccc ataaagtcgt accgacttgc ttggcttgat cgaatcagct ctcactgtta
    53281 cttgcactgg atcagagacg atacttaata cttcccctgc tgcattcagc gcctgtattg
    53341 ggacctcact attaatccca tcatctgtga ccatcgaagc gtccacatca gctactactc
    53401 gtttgatttc tgccaatgtt ttgtcacctg tagtgataga gactttttta ggactaacgg
    53461 aagtatcatc aagttcatat ccagatggtg tgaacgttga aggaatcaac gtttccacat
    53521 cgaaatcttt tattaccttt ttctcaaccg ttactgtaat agtcgatggt tcgatctttg
    53581 ctgtgacagc actgctcaaa ttttgaattt ttagcggaac ttcatgtgtt ccttcgccca
    53641 gcttagtgat atctgctgtg acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca
    53701 actgaactcg gtttgcactg gaaagtttga cagtcactgc attttcatat ccatggatat
    53761 agtagcgctt tgaatcataa gaaatcttga tcggcacccc cgtaactgtt tgattatacg
    53821 cttcatttcc agaaagcgta tttggaacgg actggccatt tgcattgaag tacagcgcaa
    53881 tactgaacag caatgcaata aaactgtaaa gaatgcttgt ccgtttctct tttgagatca
    53941 tttgcgatcc cctcccttag tcataccgtc taggaaagat tgaatcacgt ttttcttgtt
    54001 ttttgttttc tcttcagcaa ttagttctct acgcaagatt gccaaatatt cttgctggga
    54061 taagccagcc atcagttcat tatctaacgt gatgctgacg tccccggttt cttcagagac
    54121 aacgatggta atcgcgtcac tgacttcgct tataccgaca gctgcccggt gtcgagtccc
    54181 gaattcttta gggatcaaca tactttctga tagaggaaga taggcgcttg ctactgcgat
    54241 ctttccttct tttacaataa ctgcaccgtc atgcagcggt gtattaggaa taaagatatt
    54301 gatcagcagc tccccagtga tatctgcatc taaagcaatc cctgtctcaa tatactcatc
    54361 taatccagta tgacgttcga tagtgatcaa cgcaccgatt tttcgcttgg acatatattg
    54421 gatggctttg tcaaaagaca agatcatctt ctcatcttcc tgctgttcgc tttttgattg
    54481 tttgaaaagc gaactgcgtc caagatgttc cagtcctctc cggatttccg gctgaaaaat
    54541 cacgatagca gctatcaccc cgtacgtaat gacttggttc atcagccaag acaaggtgtg
    54601 cagaccaatg atttccgcta cgattcggat aacaataaaa atagctacac ctttcagaag
    54661 ctgaatcgct tttgtgcctc gcagcagttg gatcagtttg tatacaagat accagacaac
    54721 cagaatatca agtatgttca ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg
    54781 ctgccaatag tccaaattaa aaagttcccc gatttgaaat gacataaaaa gcctctcttc
    54841 tcttttgaga tttcctctct agtatatcat atgttttaat tttagaggct gatatgctcg
    54901 tatttttctt gagaaaaacg cataattttc aagaaaattg caagtatgcg ttttattttt
    54961 ctctgcaata aatattttta aacttcagag aattattcat tatatttttt ctgttatttt
    55021 tccttttttt cttttgtagc ttgttccatt tttttacgtc gttctgctct ggcagctctt
    55081 ctagcagctc ttctttcttc tgcttcaagg tccgtcgttt tttcttttct agtcttaacg
    55141 ggtacatcga tagattcctc tggttcaagt cgtttcttga ttttttctaa ttctcccgtc
    55201 agtgcagcat agttgtaaaa acgtgtttta gcgtcgtgga cagtcttttc aaataaagct
    55261 tttgcaagat ttggatttgc gttatctagc gaggaaaagc gaacttgttt cctcatgaaa
    55321 tcaggcatag cagagaaatc aggttttttg tagtccaaag tcattgggtt cttgtttttt
    55381 tcgaccattg tagggttata tcgatataat gaccaataac cagaagaaac agcttctttt
    55441 gcttctttaa gcgattcttt cattccgcca agcaagccat gtgtgataca aggcgtatag
    55501 gcaataatca gagaaggacc aggatacttt tctgcttctt caaatgcctt gatcgtttgc
    55561 atttgattgg cacctgaagc aatttgcgcc acataaacat ttccatatgt catcgccatc
    55621 attcctagat ctttctttga agcatacttt ccactagccg aaaatttagc gattgccgaa
    55681 gctggtgttg cttttgaggt ttgaccgcca gtattggaat agacttcgtt atccaaaaca
    55741 aggatattca cgtctgctcc gctggcaagt acatgatcga tcccgccaaa accaatgtcg
    55801 taagcccatc cgtctccacc aatcatccat tggctgtttt ttacgaatag atcctttctc
    55861 ttgtatatat cctccagttc agggaaaatt gatcgttcgc tttcgatcgc agcttgcagt
    55921 tttgctgctc tttgctgcgt tccttcactc tcaaacatgt gtactaacca gtcttccatc
    55981 aattctcgta gttcttctga tacttttggc aatacttctt tgattgaact tgccaaagcc
    56041 tcgcgacgag tctgtgccgc tagaagcata ccataaccaa attcagcatt atcttccaat
    56101 aaagaatttg accatgcagg cccttgacca cattcattgg tcgtataagg agaagcagga
    56161 gaagcaccgc cccaaataga agaacagcct gtcgcattcg cgatcatcat tcgatcaccg
    56221 aacatttgtg tcaataattt tacataaggt gtttcgccac agcctgagca agcgcccgag
    56281 aattcaagaa gcggttgatt gaattgtgta gacaagacag tattctttcc tttgactgga
    56341 tttgcttttt gtcttaatgt cattgcaaaa gcccaattca cagcttgttc tttctgttct
    56401 tcataaggct tcatcactaa agctttttct tttgcgggac aagcctctac acacagacca
    56461 caacctgtac agtcttccac tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc
    56521 atttctctca cgataaatcc ttctggcgct tcttccattt cctcttcatc tgctaaaaat
    56581 ggacggatgg cagcatgagg acaaacaaag gcacattcat tacacatcgt acagcgatca
    56641 ctaatccact caggtacttc taaagcgacg ccccgctttt ccatagaagc cgttcctaaa
    56701 ggcatttcgc cagttgtcat tccatttgct acaagtgtac caacagagag ttgatttcct
    56761 tcctgtgcat tcactggttc aagtatttct tgtatataac cagtgttcct tttattgatt
    56821 ttttcaggca tctccaacgt tctccaatgg tctgggatcg cgacttgatg aagcagttcg
    56881 gtcgtccgat cgattgcttg tatgtttttt tctacgatag ccattgattt atgtccatag
    56941 cttttcaggg cttcttcttt tagcagcgga agcacctcat caaaaggaat gatgtcagct
    57001 aatttaaaaa aagccgtttc cattgccgta ttgatccggc gtcccaaacc gacttcttgg
    57061 gccaatctca tggcattgat cgtatagaat ctaatgttat tttcagccaa gtaacgtttt
    57121 aattttaagg gcagatgcgt ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa
    57181 atgccacctg gcttcaatcc tttgaccaag tcatagctat gcaggtaagc tggtgtgtga
    57241 caggcaacaa ggtccgcatg ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa
    57301 cgaagatgag agacagtcag accgccagat tttttcgaat cataataaaa atagccttgc
    57361 gcatatttat cggtgtgatc tccaataatc ttgatagctg atttatttgc tccaactgtt
    57421 ccgtcagatc caaatcccca aaacttggcc tggaatgttt gcggttctgt caaatccaga
    57481 ctttcctttg gttccaaaga ttgataagtc acatcatcca cgatccctat ggtaaaccgt
    57541 tttttgcgta tgcttgggtc ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga
    57601 gtcacatctt tagaccccaa tccgtagcga ccaccaataa cagccgggcg aagttcagaa
    57661 tcatataacg cactttgaac gtctaataag agaggttctc cacctgcccc tggttcttta
    57721 cttcgatcaa gaacagcaat tgtttttact gtatgaggca acttttgcac gaatgtttcc
    57781 actggaaaag gacgataaag atggatattc aaaaaacctg ttttccgtcc gtttgcattc
    57841 aagtaatcaa tcgtttgttc aatcgtctgt gcaactgatc ccatagaaac aatgacttct
    57901 tccgcatcgt ctgctccgta ataagtcacc aagtcataag aagttcctcg taatcggttg
    57961 atctctttca tatatttttg aacgatttca ggtaatttat cataataggg gttgatcgtt
    58021 tcccgttgtt gaaaatggat gtctggattc tggttcgtac cactaacagt cggatgattg
    58081 ggattcatcc ctcgtttacg aaatgaagct aatttctcct gattgatcag aggagctaat
    58141 tcttcatatt ccaaaacttc gattttttga atctcgtgac tcgtacggaa tccatcaaag
    58201 aaattcaaaa aagggacttc tccttcaata gaagcaagat gggcaacagg tgctaaatcc
    58261 atcacttctt gtacactgct ttcagctaac atagcaaatc ctgtttgtct tgcagccatc
    58321 acgtctccgt gatctccgaa aatattcaac gcatttgtcg tcactgcccg actagcaacg
    58381 tggaaaacac ttggcagtag ttccccagca atcttgtaca tattcggtat catcaaaagc
    58441 agcccttgtg aagcggtata tgtagttgtc agtgcaccag ttttcaatga tccatggacc
    58501 acaccagctg ctcctgcttc ggattgcatt tctactactt ttactggttg accgaatacg
    58561 ttttcttttc ctgctgctga ccattgatct actaattcag ccatggtcga actgggtgtt
    58621 attggatata ttgcagcaat ctcggtaaat gcataagaaa tatatgcggc tgccgtattg
    58681 ccatccattg ttttcatttt tcgcatgatt catcatcctt gttcatattc tctcttatta
    58741 tctgttccat tataacgtaa aacatgccat ttttttcttt tatttataaa aatattttac
    58801 atgtatctat ttgaattggt catctaattt ttagacaaac agtctaaatc gttgcctttt
    58861 tttgtcagac atgtataaac tataagcgaa agaggtgttt taacatggat atatcagtga
    58921 ttgatgcaac gaaaacaaat acacaaaaag gaatccttta tggttcatcg aatgctccta
    58981 aaaaaatggt cgaattcatc aacttagctt gtccgtattg ccgacaatgg tttgaagaat
    59041 cttatgagct attagaagaa gcagtccaaa gcgggcaatt gcagcgagtg atcaaattgt
    59101 tcgataaaga aaaagaaagc ttacagcgag ggaacgtgat gcaccggtat ctcacaatca
    59161 gcgatggcca aaaagcgatc aaagagatca aacaaatatt cgatactcaa gacgagtgga
    59221 aacatctttc tctccaagaa gtagcggatt ttgcagtaga taaactaaag ctgacagaac
    59281 aaaaagacga acagctgagc caagctgtca tcaacgaagc tgagcaagca catattcgat
    59341 ttgttccaac tgtcatcttg ggtaaagaaa tctttgatga atctatttca atagaggaat
    59401 taaaagaatt gatccaaaca aaataaaaaa ataaggagcg tgggacaaaa gtaaagaaca
    59461 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcttatt
    59521 tcttggagtt aaacgcttct gtcccagcct ccttattccg aataaatggt gtccaaaagc
    59581 ttgcatctgc cagattatct catttcttat gacaagctaa gcaaattatc tccaccaaat
    59641 aatttattca tatttttgac ttgcgcatac taactaattc ttaaattttc atacaaaatt
    59701 tctcaatttc ccttaacgag taatatttat gtacggtgat tctcactagt atcccaatca
    59761 ctttttaagt tatcccgatt tcaattctac ttcaaaaata gttgctttta tgtatgctgt
    59821 tagttgatta gatggtcatt tgttgagcta ggtcgtttat tttaaacaag ttgtttcttt
    59881 agcttatacc tatctcgtct accaaacgca agatggcaaa cacaaatgca tacgcatctg
    59941 tatttgccac tttgattcgt ataaagctaa caactattaa ctcctctcag ctaatagttg
    60001 ctctcttttt tattcagatt catttttgct gatcacagcc tagtatttca atggttttga
    60061 acactaaacc aaattgttcc attacaacat tcactggctg atgaaatgga ttagtagatt
    60121 tcgattattc aaagcgattt tctattggtg gatcgtgttc ccaactaata agacggattc
    60181 caaacctatc gtacagatct tctaaaccac ttataaattt tgcacctatt tcaggtgttt
    60241 tattgcgtgg atattctatt gttacgccat taaatggata ccaattagga ttaggctctc
    60301 tttttttctg tacatctccc atagaatagt tagcactttc tgggacataa tatattcttc
    60361 gaccatcctc taattcatct aataagatat ttcgtgatcg tctatcactt gctaaatgta
    60421 gacgccacgt gcgataccat gccatgctac ctgcatctgc agaaaatcca tcaaataatg
    60481 caacaattgt atcttctatc acaatccttt ccccatctac ttcatattct aatctaaaat
    60541 caaactcccc tcgggtaatt tctggtcttg gtggcttagg acccaatatt attctactta
    60601 aaaccgcatt cgttcccaga tgaaacacag ctataacaac aataatgatt aatattttgt
    60661 ttctatttga taaatttctc attattcgca catcacctct acattctcac atattagttc
    60721 gtaatactcc tttaatatca aaatatctac tggtcgtaat aatattcgct atgaattcct
    60781 actactcgta taagaaaggc caaatcatca gcttttactt gctgatgatt tggcttgttt
    60841 ctatttatcg taattgtttt ctaatcacca aaattgttaa caatcggttc agatatttcc
    60901 cagttgatta aggtaattcc gtattgtgtc aaaacttctt gcgcttctcc gaaattaagt
    60961 acagttctta tgtatccact ttctcctaat ataaagtcta cctctctttt aaaaccttcc
    61021 tcgagatttc taagggctat atgtggtttt aatgacattg attgtacatt ttcccccata
    61081 taatattgcg cacttcctcg actaaaatag attcctagat cgtccccaaa aggtagatct
    61141 tctctaccac tagctaatct acttctccat cttcgttctc ttttcataga acctgcgctg
    61201 aactcacacc cgttaaactc agctatgaca gtatcttcaa ttatgttaat ctgtccatct
    61261 agttcgtatt caatcctaaa tggaaattct ccataggtga tttccggttc gggtatttta
    61321 ggtgagatta atgcaaagaa aggaaatatt aagcttggca acatcaaaaa aactcctcct
    61381 atgatcacaa ttcccgatgt aatccaaaat ataatttgta caattctcat tgatccattg
    61441 tattccattt tattccccct attactattc atatccgcct atctatatcg atcttccaat
    61501 gcagagataa ttgcttccat accttgattt tttactgagt tacctatgcg agatatcatt
    61561 ctccactaag gtgcatcata ttgtctatct aacatgatta attctccaat aggtgtatac
    61621 tcctccaaaa acaacattta atgcatctcc ctcgacaaca taagctacca tgtttgctgt
    61681 atatgtgttt gagtctaatt cataagcaga taaatttact gtcgctggat taaatataat
    61741 cgcattcgta tttatagcta ctgaatttgc tgcttcagca ccaccttttg aatgtccgat
    61801 cattgtgatt tcaatagtag gatttacgtg gacagccctc accgtttacc ctgaccatta
    61861 tagaaatcgc ttactttttt gaactacgca acaaaggata caatgctcct aacttcactg
    61921 tctccatttt tgtccactaa ggaatataaa aaataggcat taattcattt aattcggtca
    61981 caacacgtta ccttttaaat cattatactg tatctcatac attattatca ataaactatt
    62041 ataaaaatgc aaaaaatgct ttatcaatct attttgtaat tggtcataat atcattgaaa
    62101 tcataccctg tatcgatttt tcctatgaat ctcgtctatt tgtgattgac gaaaaagagg
    62161 caagacagac actggaatgg taattttacc cactattttg ctccaactat ttgtaatcgt
    62221 acccactttt gaaataccga gatgatcatt ttaattgata tgctgattcc caagcaaaac
    62281 cccaagaaat taaatgctct atcgcatcta gcttcttggg ttttgcttat ataaattcca
    62341 acatttaaat tttttatgct gttcggtctc agaaatgccg tttttgctta tatcacaata
    62401 tcaagatgtc aaattattcg tgttgccgat gactttgatt aatttttttc aaattgttta
    62461 ttaagactaa tgctgtcttt ttgcagatac caatgttcga actttttaac attatcatat
    62521 gatcctgcaa gttttaactt cttaccatta gatgttatca aagttataat taaccttgcc
    62581 tttttcccat taggatcttt tattttatga attttctgca ggtaatctct cctaatggca
    62641 gctattttta cctcatcagt gctgataaac cattgatcat ccacatacat actgcctgtt
    62701 atgttgtgtt tttccatttc ttccgagaac gaaaagttta aggctcgttc ttacttccaa
    62761 agttgaatga gtccatggat tggacttagt gtgagaattg agaacaacaa taagaatcca
    62821 tatcctcccg caaacatccc taaggctata ctgtctctcc atccataagt aggattagaa
    62881 gagaaaagag tcaccgtttg taataatatg aaaaggaata atccagcaca aacaccaaag
    62941 ataacaactc caaacattga atttttatac aatgaccatt tatttatagg cttttctttc
    63001 attttacttc cccctactgt tctgttcaca cggaatgaat tcaaagttca taagccctcc
    63061 tctttgaaca tatgaatacg acttgctaac tatactttaa aaaagcttgt tttaagcggt
    63121 tataggcgag ttgtaatctc tattcatctc attagcaaat aacttgccga aaagtctact
    63181 gaaattaaag cagatagtgg aagaattatt tagccaggat agacactatt tgtgaaacac
    63241 atgtctttac ttttgaaatt ctatgatgaa aagccttgga atacagtagc tcttaattgg
    63301 taatactttt tccattataa ataacctctt ttcattttat atttatcccc tctaaaaata
    63361 atatctaatc taatatttta aacttatatt tccctcgctg tgctaataga gcattactaa
    63421 ataaatagaa actagaaaaa agcattacgc ctgccagaat aaaccaatta tttgt
//
LOCUS       DAJSBM010000009        62714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJSBM010000009 DAJSBM010000000
VERSION     DAJSBM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.009"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT67_000703"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="ONT67_000703"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR2354463.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT67_000704"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="ONT67_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR2354464.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT67_000705"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="ONT67_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR2354465.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT67_000706"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="ONT67_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR2354466.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT67_000707"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="ONT67_000707"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR2354467.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT67_000708"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="ONT67_000708"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR2354468.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="ONT67_000709"
     CDS             complement(8053..8523)
                     /locus_tag="ONT67_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCR2354469.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="ONT67_000710"
     CDS             complement(8569..8994)
                     /locus_tag="ONT67_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCR2354470.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="ONT67_000711"
     CDS             complement(9061..9330)
                     /locus_tag="ONT67_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCR2354471.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="ONT67_000712"
     CDS             complement(9341..10936)
                     /locus_tag="ONT67_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2354472.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT67_000713"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="ONT67_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR2354473.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="ONT67_000714"
     CDS             complement(14489..15049)
                     /locus_tag="ONT67_000714"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR2354474.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="ONT67_000715"
     CDS             15402..16376
                     /locus_tag="ONT67_000715"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2354475.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="ONT67_000716"
     CDS             16546..17946
                     /locus_tag="ONT67_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCR2354476.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="ONT67_000717"
     CDS             complement(18024..18746)
                     /locus_tag="ONT67_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2354477.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="ONT67_000718"
     CDS             complement(18825..19331)
                     /locus_tag="ONT67_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2354478.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="ONT67_000719"
     CDS             19454..21253
                     /locus_tag="ONT67_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCR2354479.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="ONT67_000720"
     CDS             complement(21321..22895)
                     /locus_tag="ONT67_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR2354480.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT67_000721"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="ONT67_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR2354481.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT67_000722"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="ONT67_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR2354482.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="ONT67_000723"
     CDS             complement(24672..25310)
                     /locus_tag="ONT67_000723"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCR2354483.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="ONT67_000724"
     CDS             complement(25311..25961)
                     /locus_tag="ONT67_000724"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR2354484.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT67_000725"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="ONT67_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCR2354485.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT67_000726"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="ONT67_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCR2354486.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="ONT67_000727"
     CDS             complement(29104..29844)
                     /locus_tag="ONT67_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCR2354487.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT67_000728"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="ONT67_000728"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR2354488.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="ONT67_000729"
     CDS             complement(31215..32153)
                     /locus_tag="ONT67_000729"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR2354489.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT67_000730"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="ONT67_000730"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2354490.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT67_000731"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="ONT67_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR2354491.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="ONT67_000732"
     CDS             complement(35313..35618)
                     /locus_tag="ONT67_000732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2354492.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT67_000733"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="ONT67_000733"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR2354493.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="ONT67_000734"
     CDS             complement(36724..37008)
                     /locus_tag="ONT67_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354494.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="ONT67_000735"
     CDS             complement(37026..37916)
                     /locus_tag="ONT67_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR2354495.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="ONT67_000736"
     CDS             complement(37936..38154)
                     /locus_tag="ONT67_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCR2354496.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="ONT67_000737"
     CDS             complement(38124..39824)
                     /locus_tag="ONT67_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCR2354497.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="ONT67_000738"
     CDS             40091..40864
                     /locus_tag="ONT67_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354498.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT67_000739"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="ONT67_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCR2354499.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="ONT67_000740"
     CDS             complement(41688..42659)
                     /locus_tag="ONT67_000740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2354500.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT67_000741"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="ONT67_000741"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR2354501.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT67_000742"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="ONT67_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCR2354502.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(45614..46438)
                     /locus_tag="ONT67_000743"
     CDS             complement(45614..46438)
                     /locus_tag="ONT67_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2354503.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(46411..47091)
                     /locus_tag="ONT67_000744"
     CDS             complement(46411..47091)
                     /locus_tag="ONT67_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2354504.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(47114..48052)
                     /locus_tag="ONT67_000745"
     CDS             complement(47114..48052)
                     /locus_tag="ONT67_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCR2354505.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(48297..49160)
                     /locus_tag="ONT67_000746"
     CDS             complement(48297..49160)
                     /locus_tag="ONT67_000746"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR2354506.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT67_000747"
     CDS             complement(49302..51875)
                     /gene="mprF"
                     /locus_tag="ONT67_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2354507.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            52016..52822
                     /gene="proC"
                     /locus_tag="ONT67_000748"
     CDS             52016..52822
                     /gene="proC"
                     /locus_tag="ONT67_000748"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR2354508.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT67_000749"
     CDS             complement(52901..54457)
                     /gene="rny"
                     /locus_tag="ONT67_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCR2354509.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT67_000750"
     CDS             complement(54868..55917)
                     /gene="recA"
                     /locus_tag="ONT67_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR2354510.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(56026..57267)
                     /locus_tag="ONT67_000751"
     CDS             complement(56026..57267)
                     /locus_tag="ONT67_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCR2354511.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT67_000752"
     CDS             complement(57507..58085)
                     /gene="pgsA"
                     /locus_tag="ONT67_000752"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR2354512.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(58294..59217)
                     /locus_tag="ONT67_000753"
     CDS             complement(58294..59217)
                     /locus_tag="ONT67_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354513.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(59956..61242)
                     /locus_tag="ONT67_000754"
     CDS             complement(59956..61242)
                     /locus_tag="ONT67_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2354514.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(61235..62503)
                     /locus_tag="ONT67_000755"
     CDS             complement(61235..62503)
                     /locus_tag="ONT67_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR2354515.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
BASE COUNT        17307 a        13635 c        11225 g        20547 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atgaaaaaat aagaaaatag
    45541 ttcggtattt attaaaataa aaagaagcct ataaaaaatt tttcgttttt ataaggcttc
    45601 ttttctttat aatttagtta ttttttcttc tgaaggcaac caatattcgt ttgatgatat
    45661 ttacaatcag gaacaaagca ataaagacta atgtgattgt tgctcctgga ggtgtatcta
    45721 gataaaacga gctagttagt cctgacagca tccctagaaa tcctacacaa acactgatag
    45781 caatcacgac attgaagctt ttgcctaatc gcataccgat tgctgcggga agaaccatga
    45841 ttgcggatat caaaagtgca ccggcaatcg ggatcatcac agcaatagcc acaccagtaa
    45901 tcacattgaa catcatcgac atcaaatgaa cgggtagccc atccacgtgg gcggtatctt
    45961 catcaaaagt cagaacatac atcggccgtt tgaataaaaa gaacaatata ccgatggcaa
    46021 cgaataagat tcctaaaaag atgacttgct cccatgtaat ggtcacgatc gaaccaaaca
    46081 gataggattg gatacttgtt gctgaattcc ctccgctcaa attcatcaat actaacgcta
    46141 gcgccaaacc accagccatt aaaatggcaa tggagatttc tgaataggtt cggtagatcg
    46201 ttcgcagata ctccaatatc acagctgcta atacaacgac tagcatggtc gtaagtgtag
    46261 gattaaggtt caaaaagaat cctaacgcta cccctgctaa agagacatga gaaagcgtat
    46321 cagccatcag cgattgtctt ctgatcacta aaaacacacc tagcatcggg gcgattccgg
    46381 caatgaagat agcggcaaga aacgctcttc tcatgaattc atatgaaagc aacgccattg
    46441 agaatcctcc tttctcacca gtcggatctg tctgtccgca tactgtttga tatcttcatg
    46501 atcatgggtg atcattagaa ctgatttccc atggacatgc gcactatgtc ggagaagacg
    46561 ataaaattca tttctggatt cttcatccat acctgttgtt ggttcatcaa gaacaaataa
    46621 gtcagggtca gtagcaaaaa tacgcgctaa actgattcgc tgcttttgtc cgccagacaa
    46681 ttcacctatt cgccgatttc gcatatccca catccctaca gcttctaaag cacgctgtac
    46741 atgttgatga tctttttcag agagacgctt gaaccagcgg cctcttggat atcttcctga
    46801 acggactaat tcgatcaccg tactaggaaa acctgcattg aaagatgcaa cttgttgagg
    46861 aatataaccg atacttaatt tttttccttc ttgatttttc tcagcaatct ttatcattcc
    46921 ttttgaaggt ttcagtaatc ctaacgatgc tttcaccaag gttgacttgg ctgctccatt
    46981 ttctccagtt aaaatgacaa attcacccgc atctacatga taagagacat cttctagtac
    47041 aggttcatca tcatagtaaa aggtcaaatc agatatttcg atataacgca ccttacaacc
    47101 tccattctaa aacttaattg atactttctt ttaaagcttt cagatttttt tccatgacag
    47161 aaatataatt ttcgcctgct ttgatttgtt cattagtcaa gctttctaaa ggattcaaca
    47221 cttctagctt cactcctgtt tcattcgaca atgtctcagc taccttcgaa gaggcatttt
    47281 cttcaaaata aatcactttg atgttgtttt tcttcacaaa ttcttttagc tcagctaagc
    47341 gacttggtgt gggttcctga tctggtgaaa cgcccgaaat cgctacttgt tttaagtcgt
    47401 attgattcgc taaataagca aaagcagcat gctgagtgac aaatgtgcgg tttgttgcat
    47461 cttttagctc atcggagtat ttttgatcca gtgcttcaag ctttttgata taagcatcgg
    47521 cattcttcgt aaaagtttcc tttttatctg gatatttttc gcctaattct tcagcaatcg
    47581 ttctgacttc ttttatagct aataccggat cgagccaaac atgtggatcg tattcatgtg
    47641 aatgaccatg cccttcttct gattcttcat gttcgtctgc ttcttgcgat tctaaacggt
    47701 cgattccttt agctgcttcg atcatcaagg tctgtttgct atccactgaa tcctttaacg
    47761 aatccacaaa aaattccatg tctgaactat tatacacgaa aacatctgca tcggaaattt
    47821 ctgctctttc ttttgcactt ggttcgaaat catgcggctc cgttccagca gggatcaaaa
    47881 gtttcacgtt tccttcatcc cctacgatct cttttgtaaa atcatacatc ggataaaaag
    47941 tcgtaacgat cgtcaaatcc tcttttttgt ctgcttcctt gttcgtattt ccacatgctg
    48001 caaacagtaa agcacctatc attaccgtag ctgtcaaaag atattttttc atttatatgc
    48061 tcctttcaat gaacaaatcg gaattttacg atttattaac gtgaactaat gtatcaaaat
    48121 aaaaaaacac agtcaataga aatccatcaa tttctgattt aagcatgtat attcttgatt
    48181 aaatcgtaaa atggatgagt tgctgctcat atctagccat ttcactacaa taaaaaactg
    48241 aagaatacca ttttaggatt cctcagtttt tcatcaccta ttttacttct tatattttat
    48301 tgcagggttc gtactaaata aacttcctca caaaaacctt ttaacccatt atatactctc
    48361 tgcgctcttg aataatgttg acacaaggca aaaacggttg gtccgcttcc actcatcaat
    48421 gcagcatctg ctccgtactt gatcatacgc tctttgattt gatggatgac tggatgccgc
    48481 tttcccgtta tatcttctaa actgtttccc atttttttga tcatctgctg ataatcttgc
    48541 tctcggatgg cttcagcaag tccgtggatg tcttgatgtt ggatggcttg cgtatctact
    48601 tctctaaaca ctgtacgtgt agagacactc attcttggtt tgaccaacac aacccagcac
    48661 tgtggcatag aaggtaatac ttggatttct tcccctttgc caccgacaaa tgccgtactt
    48721 ccgtagacac agtaaggaac atctgtaccg acttccaaac cgatttctgc cagttcttct
    48781 aacgaacagc ccagattcca taattggttg atccctctta gagcagctgc acagtctgta
    48841 cttcctccgc ctaatccagc tgctactgga atattcttct taacaaagat cttgacaccg
    48901 gttttgatat tgtatttatt ttttagcaat aaagcagctt gataaacatt gttccgttca
    48961 tctaccggta aaaatgcttt atttgtttct acaatgattt tgtcttctgt tatttcttca
    49021 aaaaatagat gatctgccaa gtctacgctc gacatcacca tttccaactc atgataccca
    49081 tcttgccgtc tatataatac atccagtcct aaatttatct tggctggcgc tttttcaatc
    49141 aattccatcc tctcacccat tcgaaaagtc taagtcgcaa gtattctatc atagggaaaa
    49201 ataaaagtat agcttatgtc tgcctagtgg ttgattttgt catcgtttca acagctttca
    49261 cagaattttc tcttcataat ccaaacaagt tctagtgatt cttataattc tgtgagcact
    49321 tcttctgcca aactcggaac atttcctttt tcttttccta tcagactgag tagctgcatg
    49381 gtagcaaatc cgaaattact tcctttccta taagcaaaat aacgtccttc ccaatgatcg
    49441 acatatttta atttcttttc ataagcagac tggaaggaat agaaaaaatt accataatta
    49501 tatacgatat gcatgacttt ttcttgaaag aaagaaaaat ctgtttctcc aacattagct
    49561 aatggagcta agcctagatt ggcttccgag tagcccattt tctcgtatac aataaacaga
    49621 ttggctaaca gatagtcagc taattcctca gattctttag gtaagacccg caacaaatca
    49681 tatcccgcct gcttcttcgt catcggctga gcggtgatga agccaacgac tttttgatct
    49741 ttacggacaa taccgacacc gctagataat acatatgatt tatcaaatcg tcccacagag
    49801 aagtattttt ctcgtgcacc ttgaagccat ttttgtgaaa cttcatttac agcttgatgt
    49861 aattcttccg gcagttgatc gtaccaagta aaatggtatc cttctttcat caaacgttga
    49921 tattcaagtt gctgactcag ccgagacaag tcttctgttg ctagatcaac aatccctgct
    49981 tcacctactt tcgtgaagtg aaaacccaat tcatgaagca tcactacgta gctttctgat
    50041 atcttataaa acgcaagctg atagcctaaa caatctgctt gtttcataaa ctgattcgtt
    50101 gctgcccgca tcattttagg attgccaact ggatctccta aaacaaagca tttgtttcct
    50161 ttgatctgat aaccaaaaac gacttgatct tggtcgtctt cttggtaata aaaataactg
    50221 taacctgaca agcgaagctg atggcttgta gctgtgcctc cgtatgtttc caataaacgg
    50281 tcaaaacggt ctttttcgta ctcactgcca agcttcgtag tatcttcagc taaatattga
    50341 taaagcgcca ctaaagctag caatgagatg cccaaaccaa taagccctga gaaccacaca
    50401 tcatctgaag gaaacaaaat aaaatgattt cctgttattc gaccgactaa aaagcgtccg
    50461 ctgtgatagc ctgcaattgc gtaaacgata aacaaaaagc cgaacaagct agcatcaaag
    50521 attatagctc cccaagaata aacgaatttt gttcgataaa attcttttct ggataaccac
    50581 acagctaaca gaatcaagac atataccaat atcaactgcc aagataacgt acgtgtgatt
    50641 gtatttaaaa taccaaaaga tagtaaagca atcgtaggca gaaatgcctt tttgactttt
    50701 actgaaagtg ctctagcaag gccgagtaat aaaaagccaa ccaatagatt cactgtctga
    50761 tctaaaaaat tgaatgagaa cggcaataat acttgaaata gccgactgat tgcagacaga
    50821 ttagtgattg ttgatagaag taccatcatg atccccgcaa aatagacagc acttacgagc
    50881 acgatatgcg caaccttttg gaaaaacagt cgcggaatgt tatctaaaaa tcgattgatt
    50941 ttgactcctg tatgtgtgat gaacaataga agtcccgtca taaaaggtag aacataataa
    51001 aacaaccgat aatataatag ccaaactact gctacatctt gccgaacaga caattgactc
    51061 attcctaaga tcatcaaaac gtcaaaagtc cccattccac ctgggaccat cgtcagcatt
    51121 cctatcaatg tagcaatcat gaacatagga tagatcgaaa gcatcgaaat atctattctc
    51181 atcaacttgc cgatgatcaa aaataccgca agggctcccg tccattgtcc aaaggatgcc
    51241 agtagcagac caagcaagcc ttttggataa aactcgaaga acaatgtctt tctctttaga
    51301 taagcaaaaa agaaaatgac tggtgctaaa aaactaccgc cgaaaagcca gatccaatag
    51361 cttcgaaaaa tactttctga ctgaataaaa aagacatcaa taaatgtaag aaacgaccat
    51421 acagacaaac cagtcagcat aaatagtgcg acttttgata cagtggctaa aaccattttt
    51481 ctatttgtct gctttccata aaaactagcc cgcagacttg ctcctaccac accaccaaaa
    51541 ccggcgagat tgttgatcgt gtttgttacc cacgcagcaa gaagaaactc ttttctttcc
    51601 atttttggtt ttccttgttt ttctaagact ctgatcgtca cccaatcata caacaacatc
    51661 ggagtgacac ccagcagtcc agccagaatc atgagcagga tcgtcatgcg gctttgttgt
    51721 cccatccttt gccaaatatc ttgccaactc atgccattag caatatttgc gacttgattt
    51781 gccacaaaga ctaacacgga gccgaaaaaa atcagtttta aaatcagact atgttccttt
    51841 atccattgca gcatcaagta tcgtttattt ttcacatttt cccattccta acttgtatac
    51901 ttttgtgtca taccccaatg ttatcttatt tattttaatt tttccacttt agacctgttc
    51961 tttgcttgcg atgatagaaa aagaaagata tcctaatgct aaaggagagg ataacatgaa
    52021 agtaggattt atcggcacag ggaatatggc acaagctatt attgatggtt tattaaaaaa
    52081 aggaacagtc tcgaaagaag aggtgttcgt ttccagtgga cattttgaaa atgcacaaaa
    52141 atttgctgct gaaactggcg tccatgcatg caaaagtaat caggaagttg catcacaatc
    52201 agatatcgtt cttttagccg tgaaaccaaa aatcattgaa aaagtattgg aagaactaag
    52261 agaatatagc cagaaagtct tattcatttc tattgcttca ggaaaatcat taatagattt
    52321 agaaacagcg cttgaagatc ctcaggcctc catcgtacgt gtgatgccga atgtcaatgt
    52381 cagcgtagga gcaggcgttt ctgcgatttg caaaaatgaa gctgccacgg aggaacagct
    52441 tcagcaagct acgatgattt ttgaagcaat cggcagcgtc tatcctttag cagaatcaga
    52501 tttcagtaca ttcattggcc ttgctggcag ctcacccgca ttcgtttaca tgctgatcga
    52561 tgcgatggga cggactggtg tactgaatgg tttgtccaaa cagacagcca ctgagattgc
    52621 tgcaaaagca attatgggtt cttgtcaaaa attcctttca agtgataaaa atccatggga
    52681 acttgtagac caagtttctt ctcctggcgg tacaactgtt gcaggtgtag ttgccttaga
    52741 agaagcagga ttcatcccag caatcatcaa aggaatcaat acgacgatcg agaaagatct
    52801 tgagatgttg aataaaaaat aatgatagca atgttttttc gtgccttaca aaaaaagaag
    52861 agctgtggct tttgccgcgg ctcttctttt gtatttttta ttatttagca taatcagttg
    52921 cacgagtttc ccgaataacg attactttga tgtgtccagg ataatccagt tcttcttcga
    52981 ttcgtttacg aatatcacgt actaaacgaa cagcgtctaa atccgagatt tcttctggct
    53041 tgaccattac ccgaacttcg cgtccagctt gaactgcgta acttgattct acaccggcga
    53101 agctgttcga aatattttcc agacttctta aacgacggat gtagttttct aatgattcac
    53161 ttcgcgcacc aggtcttgct gctgagagcg catctgctgc agctacaagg acagagataa
    53221 cagaagtagc ttccacatct ccatgatgag aagcgatcgc gttgattact actgggtttt
    53281 ctttgtactt ggcagctaat tccgcaccga tctcaacgtg agatccttca atctcatggt
    53341 caagtgcttt cccaatatca tggagcaacc cggcacgttt tgctaattgg atatcttcgc
    53401 caagctctgc tgctaagata ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt
    53461 tttgtccata actagttctg aatcgcagac gtccgatgat cttgattaga tctggatgaa
    53521 gtgtatgagc accgacttca aacgctgcct cttccccgta ttcgcgaata cgttcatcca
    53581 tttctttacg agatttctcg accatttctt cgatacgcgc tggatggatt cgtccatctt
    53641 gtatcagttt ttccaaggtc atccgtgcga tttctcttcg gattggatcg aatccggaaa
    53701 gaacaactgc ttccggtgta tcatcgatga ttaaatcgat accagtcagc gtctcaagcg
    53761 ttcgaatatt acgaccttct cgtccaatga tacgaccttt catttcatca ttcggaaggg
    53821 taacgactga aacagtcgtt tctgaaactt gatctgctgc gcagcgttga atcgctaatg
    53881 ataataagtt tttcgctttg cgatctgctt cttctttggc acgctgttca gattctttta
    53941 ccattagagt taactcatga ttcagctctt cttcagtcga tttcatgatg acatctcgtg
    54001 cgtcctcttt tgataaacca gcaatttttt ctagttcttt ttgttgatct tcaatcattt
    54061 tttcaacgtc tttttctcgc tcttcaatta attgctgttt tgaactaagt gcttcttctt
    54121 tactttcaag tgagttctcg cgtttttcta acgagtcatc tttacgattc agcgtttgtt
    54181 cgcgttgaat caaacgattc tcttgagatt ttaattctaa cttactttct ctcagctcac
    54241 tttcaacttc agaacggtat ttctgatttt cttccttagc ttctaacaat gcttcttttt
    54301 ttagagtttc tgcttctttt tttgcactat cgatgattcc agcagctgat gattgtgcac
    54361 cagctatttc tttctcgtga cgtgactttg cgaccataaa ccctaaacca agaccgacaa
    54421 ttaaaccgat gatagcgagg agaatgttga ataccataaa atccacctcc atactatctt
    54481 tttcattttt atcatctata cagcttctat gataaattat tctaaactcg tcaatatgca
    54541 tcttcgcata tatgtgtaca aaaaacatgc acaactttat tctaatgttt gtaccccgta
    54601 gtgtcaactt taaataaaca aagaaacggc tttctactta ttcattaaac gatggttttt
    54661 aaaataaatg acactagttt catataggta aacctttaaa ttgagaaact tttctattaa
    54721 attattcttt gtagatgaac agatattttt attttatgta cccactcgta taattaaata
    54781 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
    54841 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
    54901 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
    54961 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
    55021 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
    55081 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
    55141 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
    55201 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
    55261 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
    55321 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
    55381 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
    55441 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
    55501 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
    55561 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
    55621 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
    55681 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
    55741 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
    55801 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
    55861 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
    55921 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
    55981 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
    56041 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
    56101 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
    56161 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
    56221 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
    56281 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
    56341 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
    56401 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
    56461 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
    56521 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
    56581 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
    56641 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
    56701 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
    56761 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
    56821 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
    56881 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
    56941 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
    57001 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
    57061 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
    57121 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
    57181 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
    57241 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
    57301 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
    57361 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
    57421 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
    57481 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
    57541 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
    57601 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
    57661 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
    57721 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
    57781 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
    57841 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
    57901 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
    57961 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
    58021 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
    58081 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
    58141 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
    58201 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
    58261 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
    58321 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
    58381 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
    58441 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
    58501 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
    58561 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
    58621 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
    58681 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
    58741 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
    58801 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
    58861 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
    58921 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
    58981 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
    59041 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
    59101 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
    59161 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
    59221 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
    59281 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
    59341 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
    59401 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
    59461 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
    59521 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
    59581 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
    59641 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
    59701 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
    59761 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
    59821 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
    59881 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
    59941 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
    60001 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
    60061 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
    60121 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
    60181 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
    60241 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
    60301 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
    60361 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
    60421 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
    60481 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
    60541 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
    60601 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
    60661 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
    60721 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
    60781 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
    60841 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
    60901 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
    60961 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
    61021 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
    61081 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
    61141 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
    61201 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
    61261 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
    61321 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
    61381 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
    61441 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
    61501 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
    61561 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
    61621 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
    61681 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
    61741 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
    61801 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
    61861 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
    61921 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
    61981 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
    62041 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
    62101 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
    62161 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
    62221 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
    62281 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
    62341 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
    62401 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
    62461 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
    62521 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
    62581 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
    62641 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
    62701 ttatttttta tttt
//
LOCUS       DAJSBM010000010        61479 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJSBM010000010 DAJSBM010000000
VERSION     DAJSBM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.010"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..563)
                     /locus_tag="ONT67_000756"
     CDS             complement(<1..563)
                     /locus_tag="ONT67_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCR2354516.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ONT67_000757"
     CDS             complement(750..1400)
                     /locus_tag="ONT67_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354517.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ONT67_000758"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ONT67_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCR2354518.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="ONT67_000759"
     CDS             complement(2912..3910)
                     /locus_tag="ONT67_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCR2354519.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ONT67_000760"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ONT67_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR2354520.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ONT67_000761"
     CDS             complement(4926..5657)
                     /locus_tag="ONT67_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCR2354521.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ONT67_000762"
     CDS             complement(5661..6926)
                     /locus_tag="ONT67_000762"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCR2354522.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ONT67_000763"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ONT67_000763"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR2354523.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ONT67_000764"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ONT67_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCR2354524.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ONT67_000765"
     CDS             12268..13770
                     /locus_tag="ONT67_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCR2354525.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ONT67_000766"
     CDS             13772..14509
                     /locus_tag="ONT67_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2354526.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="ONT67_000767"
     CDS             complement(14554..14727)
                     /locus_tag="ONT67_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354527.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="ONT67_000768"
     CDS             14703..15368
                     /locus_tag="ONT67_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354528.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ONT67_000769"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="ONT67_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR2354529.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ONT67_000770"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="ONT67_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR2354530.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ONT67_000771"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="ONT67_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR2354531.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ONT67_000772"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="ONT67_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCR2354532.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="ONT67_000773"
     CDS             complement(20581..21747)
                     /locus_tag="ONT67_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR2354533.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="ONT67_000774"
     CDS             complement(21763..21990)
                     /locus_tag="ONT67_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR2354534.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="ONT67_000775"
     CDS             22474..23064
                     /locus_tag="ONT67_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCR2354535.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="ONT67_000776"
     CDS             23254..23967
                     /locus_tag="ONT67_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354536.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="ONT67_000777"
     CDS             complement(24082..25092)
                     /locus_tag="ONT67_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCR2354537.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="ONT67_000778"
     CDS             complement(25129..25611)
                     /locus_tag="ONT67_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCR2354538.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="ONT67_000779"
     CDS             25761..26129
                     /locus_tag="ONT67_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCR2354539.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="ONT67_000780"
     CDS             26594..27046
                     /locus_tag="ONT67_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354540.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="ONT67_000781"
     CDS             complement(27170..28021)
                     /locus_tag="ONT67_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCR2354541.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="ONT67_000782"
     CDS             complement(28034..28819)
                     /locus_tag="ONT67_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2354542.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ONT67_000783"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="ONT67_000783"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCR2354543.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ONT67_000784"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="ONT67_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR2354544.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="ONT67_000785"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="ONT67_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="ONT67_000786"
     CDS             34217..34390
                     /locus_tag="ONT67_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354545.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ONT67_000787"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="ONT67_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR2354546.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ONT67_000788"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="ONT67_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR2354547.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="ONT67_000789"
     CDS             complement(37240..37551)
                     /locus_tag="ONT67_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCR2354548.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="ONT67_000790"
     CDS             complement(37548..37841)
                     /locus_tag="ONT67_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCR2354549.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ONT67_000791"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="ONT67_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR2354550.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ONT67_000792"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="ONT67_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR2354551.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="ONT67_000793"
     CDS             complement(39679..44037)
                     /locus_tag="ONT67_000793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCR2354552.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="ONT67_000794"
     CDS             complement(44249..45958)
                     /locus_tag="ONT67_000794"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR2354553.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ONT67_000795"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="ONT67_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCR2354554.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="ONT67_000796"
     CDS             complement(47455..48255)
                     /locus_tag="ONT67_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR2354555.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="ONT67_000797"
     CDS             complement(48252..49064)
                     /locus_tag="ONT67_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCR2354556.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ONT67_000798"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="ONT67_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR2354557.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="ONT67_000799"
     CDS             complement(49819..50541)
                     /locus_tag="ONT67_000799"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR2354558.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="ONT67_000800"
     CDS             complement(50677..51558)
                     /locus_tag="ONT67_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR2354559.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ONT67_000801"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="ONT67_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR2354560.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="ONT67_000802"
     CDS             52798..53277
                     /locus_tag="ONT67_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCR2354561.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="ONT67_000803"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="ONT67_000803"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCR2354562.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="ONT67_000804"
     CDS             54578..54706
                     /locus_tag="ONT67_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2354563.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="ONT67_000805"
     CDS             54710..55255
                     /locus_tag="ONT67_000805"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCR2354564.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="ONT67_000806"
     CDS             complement(55709..57400)
                     /locus_tag="ONT67_000806"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR2354565.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ONT67_000807"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="ONT67_000807"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR2354566.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ONT67_000808"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="ONT67_000808"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR2354567.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ONT67_000809"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="ONT67_000809"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCR2354568.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17102 a        13112 c        10631 g        20634 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    61441 attaaaaaaa taaattatat tatcgttgtt agcgtttac
//
LOCUS       DAJSBM010000011        60193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJSBM010000011 DAJSBM010000000
VERSION     DAJSBM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.011"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT67_000810"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="ONT67_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR2354569.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT67_000811"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="ONT67_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR2354570.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT67_000812"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="ONT67_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR2354571.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="ONT67_000813"
     CDS             1642..2274
                     /locus_tag="ONT67_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2354572.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="ONT67_000814"
     CDS             complement(2276..3181)
                     /locus_tag="ONT67_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCR2354573.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="ONT67_000815"
     CDS             complement(3266..3925)
                     /locus_tag="ONT67_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2354574.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="ONT67_000816"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="ONT67_000816"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCR2354575.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT67_000817"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="ONT67_000817"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCR2354576.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="ONT67_000818"
     CDS             6168..7253
                     /locus_tag="ONT67_000818"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCR2354577.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="ONT67_000819"
     CDS             7250..8314
                     /locus_tag="ONT67_000819"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR2354578.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="ONT67_000820"
     CDS             complement(8563..9366)
                     /locus_tag="ONT67_000820"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR2354579.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT67_000821"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="ONT67_000821"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR2354580.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="ONT67_000822"
     CDS             complement(10244..11251)
                     /locus_tag="ONT67_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2354581.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="ONT67_000823"
     CDS             complement(11258..11902)
                     /locus_tag="ONT67_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2354582.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="ONT67_000824"
     CDS             complement(12002..12760)
                     /locus_tag="ONT67_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR2354583.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT67_000825"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="ONT67_000825"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR2354584.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT67_000826"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="ONT67_000826"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR2354585.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT67_000827"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="ONT67_000827"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR2354586.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT67_000828"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="ONT67_000828"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR2354587.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT67_000829"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="ONT67_000829"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR2354588.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="ONT67_000830"
     CDS             18158..19027
                     /locus_tag="ONT67_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HCR2354589.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="ONT67_000831"
     CDS             complement(19153..20052)
                     /locus_tag="ONT67_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354590.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="ONT67_000832"
     CDS             complement(20217..21041)
                     /locus_tag="ONT67_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2354591.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="ONT67_000833"
     CDS             complement(21052..22347)
                     /locus_tag="ONT67_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR2354592.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="ONT67_000834"
     CDS             complement(22363..22842)
                     /locus_tag="ONT67_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR2354593.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="ONT67_000835"
     CDS             complement(22848..23876)
                     /locus_tag="ONT67_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR2354594.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="ONT67_000836"
     CDS             complement(23893..24999)
                     /locus_tag="ONT67_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2354595.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT67_000837"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="ONT67_000837"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR2354596.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT67_000838"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="ONT67_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR2354597.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT67_000839"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="ONT67_000839"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR2354598.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="ONT67_000840"
     CDS             29970..30668
                     /locus_tag="ONT67_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCR2354599.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="ONT67_000841"
     CDS             complement(30836..33475)
                     /locus_tag="ONT67_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2354600.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT67_000842"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="ONT67_000842"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR2354601.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="ONT67_000843"
     CDS             35318..36493
                     /locus_tag="ONT67_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2354602.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="ONT67_000844"
     CDS             36497..37138
                     /locus_tag="ONT67_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354603.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="ONT67_000845"
     CDS             37135..38052
                     /locus_tag="ONT67_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2354604.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="ONT67_000846"
     CDS             38049..38720
                     /locus_tag="ONT67_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354605.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="ONT67_000847"
     CDS             complement(39261..39749)
                     /locus_tag="ONT67_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCR2354606.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="ONT67_000848"
     CDS             complement(39950..40216)
                     /locus_tag="ONT67_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2354607.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="ONT67_000849"
     CDS             complement(40216..40479)
                     /locus_tag="ONT67_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2354608.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="ONT67_000850"
     CDS             40955..41140
                     /locus_tag="ONT67_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2354609.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="ONT67_000851"
     CDS             complement(41282..43105)
                     /locus_tag="ONT67_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR2354610.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43703..46408)
                     /locus_tag="ONT67_000852"
     CDS             complement(43703..46408)
                     /locus_tag="ONT67_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCR2354611.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46714..47661)
                     /locus_tag="ONT67_000853"
     CDS             complement(46714..47661)
                     /locus_tag="ONT67_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR2354612.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT67_000854"
     CDS             47851..48120
                     /gene="rpsN"
                     /locus_tag="ONT67_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2354613.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48296..48658)
                     /locus_tag="ONT67_000855"
     CDS             complement(48296..48658)
                     /locus_tag="ONT67_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2354614.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48669..49790)
                     /locus_tag="ONT67_000856"
     CDS             complement(48669..49790)
                     /locus_tag="ONT67_000856"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR2354615.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49806..50156)
                     /locus_tag="ONT67_000857"
     CDS             complement(49806..50156)
                     /locus_tag="ONT67_000857"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR2354616.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50286..51797)
                     /locus_tag="ONT67_000858"
     CDS             complement(50286..51797)
                     /locus_tag="ONT67_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2354617.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52119..53471)
                     /locus_tag="ONT67_000859"
     CDS             complement(52119..53471)
                     /locus_tag="ONT67_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR2354618.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53624..54700)
                     /locus_tag="ONT67_000860"
     CDS             complement(53624..54700)
                     /locus_tag="ONT67_000860"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR2354619.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54896..56641
                     /locus_tag="ONT67_000861"
     CDS             54896..56641
                     /locus_tag="ONT67_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCR2354620.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56921..57358)
                     /locus_tag="ONT67_000862"
     CDS             complement(56921..57358)
                     /locus_tag="ONT67_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR2354621.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57342..58715)
                     /locus_tag="ONT67_000863"
     CDS             complement(57342..58715)
                     /locus_tag="ONT67_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354622.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59037..59525)
                     /locus_tag="ONT67_000864"
     CDS             complement(59037..59525)
                     /locus_tag="ONT67_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2354623.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59547..60149)
                     /locus_tag="ONT67_000865"
     CDS             complement(59547..60149)
                     /locus_tag="ONT67_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2354624.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17104 a        13016 c        10989 g        19084 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact ttaagaaaat aattggaaaa aaacagaaaa atatcaagcg ctttttcaaa
    43261 cgctttaata cagcaaaaat ctaacatttt tttatgggct ttttctttga attctataat
    43321 aaaaggatag aacttgactt agaaaatcaa gttctatcct ttttgatatc ttaacttttg
    43381 gaagagagat gtaaggctgc tttctaccca tcattcttac tactcgaagc atagcgcagt
    43441 tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat ctgcggtaat aagcccaacc
    43501 aaattaaaaa cataagaagc tgtttttatt tggtcgttct tattacttga tgcagagcag
    43561 cttttttaca acctcttggt acacggtgtt caatcagctg accctcggta ttagctcaaa
    43621 aaaacggcaa aatatgagga actatttttc catttttttc tctaatactc aggtcaaaac
    43681 actgatttca caacctcttt ttttaatgaa aaatatggct ctcatgcgcc gcttctttga
    43741 atttcgtcat tttttcttcg atgaacggat agattgccgt tcctacgatt ccaaagacaa
    43801 caaacaaacc gatcatgatc cagattgctt tcgtagcatt tggccaataa attcctcctg
    43861 cagattcccg taacaagttt accgcatgag taaatggcaa gaacggattg atcatttgga
    43921 agaatttccc tgagacttgg atcggatagt tcccgcctcc gccagagatc gacagtacta
    43981 agatgataat agcgatccct ttaccgacat tatcgaataa tgcagccaag acatagacga
    44041 tcatcatgaa cgctaaggcg atcaagacag caaatgacac gctgtagacc ggattttgga
    44101 catctactcc tagtaagaaa atattcccta atgtaacgat caagctttgc gcaatcgcca
    44161 ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg tttcgtaaaa ttgtttcggt
    44221 ctttctttcc taaatagtat tctgtcgtag caacacttga aagaagaaca gcgccgaccc
    44281 acagacatag tgcagtataa aacggggtgc tagctgaacc gttatttgcg attggataaa
    44341 gcgcattggt ttgtagctca acaggttgtg taaagaagtc actttctttt gttgcgtcta
    44401 gtttcaataa tttgatgact tcgcctagat cgacttcttg ttcgcctttt cggatcgcgt
    44461 ctgctgcttt ttggatacca gctttgatat ttggccagtc attgttgatc aagtccgcag
    44521 cagcgttcac agctgtttcg acttctggca ttttttcatt tactgttgtc aatgtaccgg
    44581 tgatttcatt ttttagtgct ggccaatcat tttggataaa atcagcggcc aatcctaatt
    44641 tttgctgaat gatcggtaat tcattgttgt ataggtctgc cccttcattg atgccattga
    44701 cgatggtatc catatttcca ttcagcattg tattggcatc atggatttct tgtccaattg
    44761 caggcatttc tttttgatat ttttctaaaa ttgccactgc attcgttaca gttgctttcg
    44821 ttgagctgag aagcgaatca aagtcgatct ggtccgcttt gctcagtgcg ccttgtgcag
    44881 tcccaatcgt atcgatgact tggtctagta gagaagtgac agtggaactg atttgacctg
    44941 tattgatacc agcaatgatg ccattgatat tattaacaac ggcatcaatt tgatctagca
    45001 attcatcaat agccttcgtg tcttcatttt gaacagctgt gttcaaagca gataggcggt
    45061 tttgcagatt gcttagcaag ggttttaatt gatttagctg atcaataatt ggctgtaatt
    45121 tcgtatttcc agcacttgtt tgcaatttag tcaggaactc gaccagttta tcgattgcat
    45181 tttgttgttg agatattccg tcaataaagc cgttcaatac attgctgatg tttttctttt
    45241 catcatcagt cagcttacca tcttcaatag ctgttcttac agtatctatt aacgaacctg
    45301 tcgttgttgc cacttgtcca attgacttca atgtaacatc tacactgttt gtgatgcttg
    45361 gtatcgcatc tttcagctga gaggcacctg attttgtttg gtttgctaga tcacttgcct
    45421 gattttctaa atttttcaca tctggcaaga tatcttgtac ttgttggatg atcgtcaagc
    45481 cttgttttgc ttcatcgatt ccttggttca ttgtctcttg gatcgaagca aaatcgccgt
    45541 cgatctctgc tagttgctta ccggcatttt ggatctctgg gattttttct tgcaaatcaa
    45601 ggatgatttt tgcttgatct ttcaactcag gcattttgtc attcaatgca acgacttttt
    45661 cacccatctc atctacttta gggatgtaat ccacgaattc atttgcttta ttcagttttt
    45721 ccttgatttc aggtagttga ctttgtaatt cgaggacttg ttttgtgtag ccgtcgatcg
    45781 tgtccaaatt ttcatctgtt gataaaatca tatctttaac tttattgata cttactaggt
    45841 tcgtatcgat gtcgtaacct atctcattga atactttcaa taaagtcgaa ctggctgttt
    45901 tgataaattc atttgtgatt tgcgattgga tcgaacttgc tcctttatcg gtgattttcg
    45961 gtgcaatcgc attgattttt tcatttaccg tatattcgat ttttggtttc ttgatatcac
    46021 cactagtaaa actcagcaga tcttctgaga aatcctttgg caagtaaata cctgcgtaat
    46081 actttccgga acggactccg tcttctaatt gttcttttga atcaacgaat tgccatccta
    46141 attgtttatt tttatgaagc gattcgatga cttgctcccc aatggccact tctttacctt
    46201 gaaactctgc tggcttgtct gcactgtaaa cagcaattgg cagctctcca gtatttccat
    46261 atggatccca taatgctttg atgttaaacc acgcatatag cgaagggatg atcatgatcg
    46321 cgatgatcag aagtgttgca atcgggtttt tgaaaatccg tttccagtca agcaaatata
    46381 atcgaagcgt atttttgatt gactgcatag ttctccccct tcttcttttt tcagctgaat
    46441 tactttacca tgtatttcat acttttttca atcggctttt ttcaaaaaag tcgaattttt
    46501 ggacatatca cggcaattgt ctcattgtaa tctcttacat attgagaaat aattaaggat
    46561 tacagccttt actttatcat gaaataaaag ggaaagataa ttttatgcca aagtttttat
    46621 ctattatacg tatacgcaaa aaagctaaaa cattttttgt ctttgcccct acaaaaatgt
    46681 ttcagctttt ttcgtttttt attcctgtat ttcttatttt tcgaatgaca ggatgatttt
    46741 cccagcagga ggatgtcctt caactagctg atgtgcttgg atgacatgct taagtgaagc
    46801 agacagattc attgaaataa aaacatgaaa ggctcctttt tggtaggctt tgattatgcc
    46861 agcaaaggct tctgcatcta gatattcctt tcttggaaca aacgattcat aaaagccttc
    46921 ctttttttgc cgcaaatcta gatctgggag gtcatttaat gcaacatacc tgccgcctgg
    46981 tttcatgatc tggatgccgg tttctccttt gatacttcct ttcgtcgcat cgattacaag
    47041 gtctgcctga tcagcaaatt gcagacctgg atttgtttta tcataagcag caaatgctga
    47101 agctcctaat ttttttacct tttcctcatt tttactggat gcactggtta agattcggat
    47161 tcccttttca tggagaagct ggatcagact tgacccgacc gcacctgatg ctcctagtat
    47221 cattacagta tctgtcggct ggatttccgt caaatggttg attagattat aagccgtaat
    47281 tcctggcgtg accattccgg ctgcttcttc ccaactcatt ttatctggaa tttttgctac
    47341 ttttgctgat ggcaacacga cttcttctcc gtaagtcccg ccgactgcat ggacagctac
    47401 tcgatcacct acatgaacag ttgtgacatc tgaaccaatc tctgtcacaa taccagcgcc
    47461 atcattacct ggtacataag ggaatttcaa tgttcgtatc tctttcattt ctcctaaacg
    47521 caaggcaaca tcataaggat tcacactgaa tgcttttatt tctacacgaa catgtccaga
    47581 accgacttct ctgggatgag catcgatttc ttcaaaaaca tcttgtgctt ctccgtaacc
    47641 gttaattgca aatcttcgca tggtcaccat ctcccttttt ctttaagttt acgtttatgc
    47701 ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca tgattcaaaa caaaatagtt
    47761 gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag cgtaaagatt acgatttacg
    47821 ctaataaaaa ataattggag gaatttgttg atggctaaaa aatcaaagat tgccaaagca
    47881 aaaaaacagc aagaaacgat tgaacgctat gctgctttac gccaggaatt aaaagcgcaa
    47941 aaagattacg aaggcttgcg taaacttcct ttagatgctc gtccagaacg attgaaaaat
    48001 cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta aattcgggat gtcccgtata
    48061 aaattccgtg aattagcaca taaaggtttg attccaggag taaaaaaagc cagctggtaa
    48121 tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga ccactccttg gatcatcaaa
    48181 aaagatacgt tcgcttcttc tagcagaaat gctggtacta gaagcagacg tatctttttt
    48241 tacgcacagc tggtcctttt ttatatgtaa aggctgcatg gttacttcgc tatatttatt
    48301 tagtaacagg agtcagtgct tcgataccca cactaatttt caatgctcgg tcaacttctg
    48361 ccataatggg tcgatccaaa tggcagacct tttctttcaa tcttgattta tcgatcgttc
    48421 tgatctgctc caacaggata acggagtctc tttctattcc tgttccagta gctttgatcc
    48481 caatgtgagt cggaagctta ggtttagcca tttttgccgt tatggccgca acaatgatcg
    48541 tcgggctaaa atggttacct agattatttt gaataaccaa aactgggcgt gttccgcctt
    48601 gctccgatcc cacgactgga gaaagatcag caaaataaat atctcctctt tttaccatag
    48661 gatcctcctt attcttgata ttctcttggt atccgctgtc ctaatccgca agccacttca
    48721 tagtggatcg tacctaattg atctgctata tcctgcatag tgatttcttt gtctgcattt
    48781 ttgccgataa gcgtcacttt tgttccgaca ggaaattctt gaggcaatcg gatcatcagt
    48841 tgatccatac atacacgccc aatcgtttca cagtagttcc cttctaccaa tagagaaaaa
    48901 ccctgcatct ttcgtggcca gccatctgca taaccgatcg gtatcgttcc gatccactct
    48961 gcttcaggag tgatatatgt ttctccatag ccgattcctt cacctgctgg gagttttttg
    49021 acttgaatca attcagaaac cagctcaagt gctggctgca atggataaac ttccggtagt
    49081 gcgtgcccag agggattaag tccgtacatt gctactccat aacgaatcat attgccgatt
    49141 gtttcatcat gccataaagc cgttgcacta ttgctgacat gtacatatcg aggaagttcg
    49201 ggaagttttt tcagcacttc tataaagcgt tctttttgca gattccaata tgtatcatct
    49261 gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc attctaatac acgactttct
    49321 ttgatgaatg cagctgcctc ttttacttct tcaggtgtac aaaatccgat acgacccatc
    49381 ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta gcttactttt ctctaaaaca
    49441 gcagctgctg cttgcaacca ttcccgtgta cctgcagtta ctgataaatc atgagcaaca
    49501 gctacagtca gtgcgtcgta aggaaccata ttcaagataa ggatgggctg agtaaatcca
    49561 gcttctctca actcgactcc ttcatctaaa tttgaaacac aaaagcccga agctccacct
    49621 gcaactgctg cctcagctgt ttcaatagct ccatgaccgt atccgttcgc ttttactact
    49681 gcaaacaatt cttttccttg cggaagtctt gcaacttcat ttgctacatt ttcagttata
    49741 gcttttttat gtatcactgc tttcgttggg cggtgccaag cgacaaccat cgtttttcct
    49801 tctttctagt cttccaaaat aatttgtgca aaagctgttt catttgtatg agtaatactg
    49861 acccatacat tgccttcatg aggtgaacgc gtcacaacag gctgaccatt ttcatttttt
    49921 aaaatctcga catcttgaaa ggtaactttt ccgatcccag ttccccaagc tttagagaaa
    49981 gcctctttgc aagcgtaacg cccgcctaaa aattctactt ggcgatgaaa aggcaaggac
    50041 tggaatagtt tcatttcatc agaggtcaag attcgtgcaa tgaattttgg tttttcttcg
    50101 atcagccgag tgatcctagg aagttcgact gcatcgatcc caattccttt tatcatatgc
    50161 ttttctcttt tcaataaatt tagaacactc tcattctacc aaattataac cattttgcca
    50221 caaataagtc cacaaataac aaaaatgaaa gtttctctta aaaaagagaa actttcattt
    50281 tatgtttaat cgttatttga acggattaca aatccgcgtt ttttatcatt gtgacgttta
    50341 ccgccatctt tttggctgtt attatttttg ctataaccgc cttttgattt gtttccacga
    50401 taattaccgc cgtcacgacg attacggttg tttccgccac cacgtttgcc atttttattg
    50461 tatccttttt tctgcattgg caatgggcgt tctggtgtga tcttcactgg cactgcatca
    50521 gctggatctt ttgctgtcgt ttttagcaaa agtgcaacta aatcttgtgc agaatattct
    50581 tctaataatt tatctgctgt ttgcaggtat ttatctagac cattttctgc aagttttgtt
    50641 tcgatttgtt caactgcagc acctaattga cctttgaatg cttctttttc ggttggtgga
    50701 cgtaaagtcg tcatccgttt tttcgtcaag ttttcaatca catgcaagta gtccatttca
    50761 tttggtgtaa cgaaagtcac agacataccg ccttttccgg cacggcctgt acgtccgata
    50821 cggtgaacgt agctttctgg atcttgaggg atatcatagt tgtacacatg agtgacacct
    50881 gaaatatcta atccacgtgc agcaacgtct gtcgctacaa ggatatctaa atgaccgctc
    50941 ttaaatgaac gcaatacgct cattcgtttt tgttgagaca aatcgccatg gattccttct
    51001 gcacggtaac cgcgtgcttc taatccgcgt gccaattcgt ctacacggcg ttttgtacgt
    51061 ccgaagacga ttgttaattc aggtgtttga acatcaaaca agcgagtcat gatatcgaat
    51121 ttttcgtatt cttttgcgcg gacatagtat tgatcgatca agtcagctgt catttctttc
    51181 gctttgatct tcacatgatg tggatttttc atgaatttaa cgccaatatt cttgatagct
    51241 ggcggcatcg ttgctgagaa aagtaatgtt tgtctttgat caggaacttg tgaaatgatt
    51301 ttttcgatat cttccaagaa gcccatattc aacatttcat ctgcttcatc caaaactaat
    51361 gtttgaacgg ttccaagttt caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt
    51421 gtacccacaa cgatatgtgg acgatctttc aagccacgga tctgacggcc aatatctgct
    51481 ccgccgtaaa cggcttgtac acgtactttt ttgtcgcgcc ctaaacgata taattcttct
    51541 tgtgtttgga tagctaattc acgtgttggt gcgataacta aaccttgtaa ttcgtgacga
    51601 tctggatcga ttttttctaa cattggtaat ccaaatgcag ctgttttccc tgtacctgtc
    51661 tgtgcttgtc caataacgtc ttttccagct aatgctagtg gaatcgtttc tgcttggata
    51721 ggcgttgctt cttcaaaacc agcgcgttcg actgatttca acaattctgg tgataattct
    51781 agttctttaa atttcaaatg aatcctccta tatggttgtt aactgaggat gtagcttttt
    51841 tgctaccccg tcgatttgtt cggtcaaagt gtattgtttc tttttgacag tggccacgca
    51901 cgtttttatc cattcttagc ccctgtggca ccaccgaaaa atcactttgc gaagtttagg
    51961 cattagaaaa gccaaacggg tcgcgttcag ctttatcatt attatgacat ctggcttcaa
    52021 caagttgttt ctatggcaaa accagtccaa tttctcttca tctttcggca ataggttatt
    52081 ctaacatagt ttttcgtttt ctgcaagttt acagattttt atttcatttg ctgaagttgt
    52141 tctaccactt caaataagcc catgctgttg ctgcctttta gcataatact atctgttgct
    52201 tcaagatttt gtttgatagc atcaatcaat ttattctttt ctttttcaaa atagaacaca
    52261 ggtagttggg ggtatttttg gttcaattct tcttttagtg catgcatttg ttccccataa
    52321 aggaaaatca tggcgaattt ctcaggatcg atatgttcac tcatttgcgc atgcatattt
    52381 aaagactctt tccctaattc cagcatatct gctaacacag cgattctgcg tccttttgtt
    52441 ggcagatttt taaaagtatc taataccagt cccatggctg ttggatttgc attgtagaca
    52501 tcgctcaaaa tagctgcacc atttcctgcc gttagccatt cggttctgtt ttgagtgata
    52561 gaaacatggg cgagcccttg gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc
    52621 gtatacgcaa tcaaagcatt cgttacatta taacttccag gcagcgggat cgtaaatgtt
    52681 tctccttcta ctacaaaatc ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca
    52741 tcgccatttc caataccgaa tgttgtgact gtttgtacta atgttgccgt taaaggtgtc
    52801 agcaacggtt catctgcggg cacaacgagt aatccgtctg atacaagtcc gtcagtgatt
    52861 tccattttgg ctttagcgat tccttctcgc gacccaagat tttctatgtg tgcttcaccg
    52921 atcattgtga ttgctgcagc ttttggttga gccataagtg aaagttccgt gatttcattg
    52981 gcgtgatcca tgcccatttc caaaacgatc ttttctgtat cttggggcat gtgcaaaatc
    53041 gtgtaaggaa gaccaatatt attgttgtaa tttccttgcg tcttatatgt tttgtatttt
    53101 tcagacaaga cagacgcggt tagatctttc gtggtggttt taccattgct tcctgtaatt
    53161 gcaacgactt ctgcgcctac tttttccaag taataggttg ccaattgctg catggctttc
    53221 aaaggatctg ctacaggaat aactggaaaa ttttttggag cttgttcaac aggcaagctc
    53281 caaaatgaag cagatgctcc gtttgtgatc gcttgctgga taaattcatg tccatctcgc
    53341 gtaccagcaa gaggaacgaa taaacttcct tcggttatct tccggctgtc aaattcgaca
    53401 ttggtaatct ttgtcagtgc agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt
    53461 gttagattca ttgagatctc tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg
    53521 cgggttcgca tgactctcac ttccaagtaa atttctctat tcgtatttag aagctagaat
    53581 gaacgcggtt acgcaatcac tccagcctct tctttatttc tctttattca tttttttcaa
    53641 aaaaagattg acgctgatgg tatcgattca ttcctaactg gatcagttct tcaatcaaat
    53701 caccgtattt caagcccata ttttcccata agagtgggta catactgaac tccgtaaatc
    53761 ctggcataga gtttaattca ttcaggaata attcattttt atttgtcaaa aagaaatcgc
    53821 accggctcaa tccgcttcca cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt
    53881 tttgataaac ttcttctggc acttcggctg gaatctgcat ttcgatttta ttattgatat
    53941 attttgcttc ataatcatag aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc
    54001 gaacgtcttc atttcctaat acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa
    54061 cgattgctcg agaatcatac tgataggctg ttgctaaagc attttgcagc tcttctcggt
    54121 tttctgcttt tgtaatgccg acactagaac ccatattcgc cggtttgaca aacatcggat
    54181 aaagcaaaga accttcacat tgatcaaata cttttttagg attttctttc cattgattct
    54241 taagtactgg tacataagga acttgcggca caccagcagc ttgtaaaata tacttggtca
    54301 tgattttatc catggcacat gcactggtca atacgcctgc gccgacataa ggcatattca
    54361 atgtctctaa gaagccttgg atcgttccat cttccccgtt tggcccatgt aaaactggaa
    54421 aaacgatggc tccttcttct ttgatttcgc ccggattgat cacttttcct gtaaagcctt
    54481 cctctgtctg tccacttggg tcccatgaaa gatgcaagac atctttgctg gcaggttttt
    54541 ctgttagtaa tggacctttg acccattgtc cttcttttgt aataaatacg agttgaactt
    54601 ggtaataatt ataataaatg gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat
    54661 gctctgcgct gcgtccgcca tatagtaaag taatcttcaa aagattgtcc tcctgtattc
    54721 taaatcgttc agtgatttac tctcatattt tagcacaaaa caaaaataaa cgaataactt
    54781 atttataaaa aaacgagcca gcttctaaaa gaacgagcgt catttatttt ttgtcagcta
    54841 gattatttgt cccgtttttt ataaaaagtt atgataaaga aaaggaggga tgatgatggc
    54901 gcaaacaaat ccacgcagcc gcttggcaga aatcattcga gtgttcatca attacaatgt
    54961 agtgccaaac tttgtacagc aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact
    55021 aggccccacc ttcatcaaga taggacagat gctttctgtt cgagaagatt tgctttcttc
    55081 tgccttcact caaacattca aaacattaca agatagtgta cctagcgaca cattttcaac
    55141 ggtcaaaaag acgatcgaaa cagaactttc tctttcatta tcagatatat tcgatgattt
    55201 ttcaaaatcg ccttttgcct ctgcttctat ggggcaggct catcgtgcca agctgaagaa
    55261 cggtgacgcg gtagtcgtta agatccaaca ccctaacatc gccgaagaga tccgacttga
    55321 tcttcaacta tttgaacgag cgatcccttt gatcaaatac attcctgaaa ctagcgtagt
    55381 tgatctaaaa ggggtactcc aagaagtaaa acgttccttg atcaatgaaa tggacttctt
    55441 aaaagaaagt caaaatggcg aacaattcta tcaaaagaac aatggctgga aagagatccg
    55501 cagccccaag atttatgacg ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc
    55561 tggtaaaaac ttgaatcatt taatgaacgc cgaaaacaaa actgaaactt ttatcactgg
    55621 aattcaaaac aagcagctga aacaagaagt tgcaaagctt ttagttgaga actttatgaa
    55681 acaggtgttt gatgatggtt ttttccatgc tgatcctcat ccagggaatc tgctattcca
    55741 tgtactaaca aaagaagaac agacacaagc tagccgaaaa actgaaacag tccatgaaaa
    55801 agagttcggt tcctttgctt ttcgagcctc cacttctgca gaagaccctg ttgcgccata
    55861 taccatcaac tacatcgatt ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa
    55921 actcacacaa gccgttttag cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt
    55981 ccttcgactt tgtcagcaag aaggctcgtt tgatgaaagt cggttccacc aagaactgac
    56041 aagttttctt gaacagtatt atgattctcc tattgatgaa atcaacttgc aagaagtatt
    56101 cacacatgtc gtgacgattt gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct
    56161 tttgctgaaa gcatttggga cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc
    56221 cctcatggaa gtcgcttcgc cttttgctca acattatttt ctcactcatc tggatgtaga
    56281 agatacattg aaacaaagcg gattagactt attggaaggg atgaaggctg cgcctaagat
    56341 ccctcaacag ctccaccatc tattagaaat gtggacttcg ggacaaggaa aagtcaattt
    56401 ggaattgaaa aagcaagaca aattattgag caggatcgaa agtatgatca atcggcttgt
    56461 tttcggtatg attttagctg cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc
    56521 tgtagaaaac gcagaggtcg tttccttatt agggatattt acgtatgcta ttgccgcatt
    56581 cgtcattatt tttttggcaa tcgatgcatt gatccaaatg tataagaaga ggaaaaagta
    56641 gaaaaaggtt gtgacagaaa gaatcagctt caagaaataa gaaggggttt tacataaatt
    56701 gtttctcaaa tttttgtgaa aattcggctt atttttcgaa gaggctactt ctgaaacacc
    56761 gagaataaaa aatgtgaaaa agctgttctg cacccagtaa taagaacaat caaaaaggga
    56821 acatggaaca catttatcct gtttccctgt tccctttttg atgtattcga gcataaataa
    56881 aataccttcg ttacctacta tcttctgtac ttggtaaacc ttagtcaatt gcctttttta
    56941 acgcttgacg aaaagacata tgatctttct ccgtaaaggg ttttggaccc tttgtcctta
    57001 tgccagcttt tcttaacttt ccactatgat aacgttgttc tagaagcgtg ccgatattcg
    57061 cttcgtcaaa ttcttttcca gagtgatgga acactcgcac acccttcggc aatacaagtg
    57121 aagctaaacc ataatcttgc gtgatcaaaa agtcattctt cttaaccata ccgacgattt
    57181 tataatccgc actatcagct ccgcggtcta cgtagacgat ttggacatga ttagcgtatt
    57241 ctttggtcgt ataatggtcc acactggtca cgataaccac gtcaagacca taggattctc
    57301 ctaattttat gacatcttct tttactggag aaccatctcc atcaatgaat agcctcattc
    57361 tttttcgcct tcttccataa atgataaaca gttactaatc cgctcagtaa aagaccaaca
    57421 ttgatccatt ttgccaagat aagcagttgt acactttcta tcggcactga ttcatggttc
    57481 agcgtgatca ataatagatg gattggctct cccatttctt ttgctaaaac aaacaaaagc
    57541 gtcatcaata aaaactggtg gatcacactc cctttatttt gagtagtata cttgacaacg
    57601 atccggcgat ccattggaat agctgtatca gtttgataat attccttatt tagccagctg
    57661 cctgttacta cgtgccagaa actaagcaga aagacaccta tcgcaaaacc atttggaatc
    57721 aagaggacca ataaagaaga actcagtcca agcaattgca tagccaatgg agaccatgtt
    57781 aaacgtttca aaatccatgg tccgactatt tttgctgcaa tcaagcctgc tgcataaggc
    57841 aaatacataa aaatcgaaag tttttctaat ccgtacagta tgccaacata aaaactgcta
    57901 aataaaaaaa ggaaattccc tagcattcca tttagaaaag taaacagatt tagccagctt
    57961 ggcaacttcc attccttctt ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc
    58021 aagaaaccat aaatagcaag atcaaaaagc ctttcatcga ataacaatcg ggcatttctc
    58081 aaaagaaaca ataagccaaa gaatatcaaa aataaaacaa gctctcgcgt agctaccaga
    58141 tttcttttga tgtctttgaa atccagttcg taacgcggat aatgactaac cgtatggtag
    58201 gccatgacgt aaaaaagtgt ataaatagta aagcttgctg caatctgagt cggtgcaggc
    58261 aactcaatcg cttgatacag gagaaatagc cagatagctg caaataagta ctttttccca
    58321 tccatattga taaaaccttg cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca
    58381 agccatgctg cactcagccc tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat
    58441 acgcctaata ttccaagcaa tgaaccagca ccaccaatca gcagagcgat catcagcaac
    58501 gtgtaactat ctagaccaaa tcgtagactg ttgactaaaa atagtccagt catccgaaag
    58561 gcataaaaca gcgtaaatgg caaaatcgtc agccaattat gcgagtctgt tagtcccaaa
    58621 aataaaagat aaggaataaa aataaccgtg ttacttaaga taaagggcgt tgtttgtgta
    58681 aaattacgcc caaatcgttt gagtttttcg aacatgacaa tggctcctta aacagtctta
    58741 taactaatct gctcttcgat tttagcatta aaggggaaat aaaccaattt tacagaatat
    58801 attcttgtaa aaaaaaatac agaaagaagc ataagcttct ttctgcattt ttacggtact
    58861 tgattactca agcagacaag cccttctcca caaaagtctt gcccgacacc cctgcatccg
    58921 gcaggtgata aagatagaaa tcaattatcc tgtgcctcca aaatcaatta ggagctctgt
    58981 aaaccagagt ccttctcgac ccgtcgcata aatttacttt aacacagttc ctatgtttat
    59041 gcgagagctt ttttcatttt ataatcgacc atttgaaatc cttgaccgat atctgaaata
    59101 agttcttctg taatctcaaa tccttcatgc ttataaacct cgattgcttg atgattcccc
    59161 tgattcactc gcaaaaagat ctctctttcc tttgtttgcg cagcaagttc ttcataccag
    59221 cgaaaaactg aagaagtcaa tccttgacca cgtactttat ttagcaaata taatttactt
    59281 aaataaattt ttccatttat ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt
    59341 tttaataaaa agtaggacac accttctgtc agttcttttt gaatctgttt ttttgactga
    59401 taagttttca gcatgtaatc cacttgtttt tgaccaatga tcggcacaaa aacttccggc
    59461 catacttctt gtatgactgc catcagctca tccagttcat tttctgttac ttttattttt
    59521 tccatacacc cacgtccttt tttacattat aaatgacctt gcgcttgatg gaataaaaag
    59581 agcatgatca tgctatacac acccaccgac cacggtttca agaaaatcac aatctgcata
    59641 aattccctaa aagataattt cgtcaaacca gctactagac agatcaagtc gtctggcgca
    59701 aagggaagca gcaaggtaac aactagtact tttttaatat ctttcgtttt tcttgtcagc
    59761 aattcatcaa atttctgata cttttttggc gataaaataa gctggacaaa ggctacgcca
    59821 tagtaacgga caagcaaaaa tccgattgct tccccaatca ctaggccaat acaactatat
    59881 aaaagcccaa atccattgcc aaacatcagc atgcccgcta ctgaggagat ccctcccggc
    59941 aaaataggaa tgatcacttg gatcacttgc aacagaacaa atatcatcac tgcatacttg
    60001 ccaaattgct ggataaacgc ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa
    60061 gcataaagaa tcaacacaaa gaaaaaaaac agaccaataa tgggcatgat ctgaagacat
    60121 ttttgtaaaa ctgatccttt tttagacatc tatattcccc acatcttttt tcatttatta
    60181 aaactgcctc ctt
//
LOCUS       DAJSBM010000012        58961 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJSBM010000012 DAJSBM010000000
VERSION     DAJSBM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.012"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            591..1280
                     /locus_tag="ONT67_000866"
     CDS             591..1280
                     /locus_tag="ONT67_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2354625.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ONT67_000867"
     CDS             1267..2475
                     /locus_tag="ONT67_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2354626.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ONT67_000868"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ONT67_000868"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR2354627.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT67_000869"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ONT67_000869"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR2354628.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ONT67_000870"
     CDS             complement(6578..7273)
                     /locus_tag="ONT67_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCR2354629.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT67_000871"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ONT67_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR2354630.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ONT67_000872"
     CDS             complement(8472..8663)
                     /locus_tag="ONT67_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCR2354631.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ONT67_000873"
     CDS             complement(8684..9574)
                     /locus_tag="ONT67_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR2354632.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ONT67_000874"
     CDS             complement(9561..10328)
                     /locus_tag="ONT67_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2354633.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT67_000875"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ONT67_000875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR2354634.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT67_000876"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ONT67_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR2354635.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT67_000877"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ONT67_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR2354636.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ONT67_000878"
     CDS             14899..15282
                     /locus_tag="ONT67_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354637.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="ONT67_000879"
     CDS             complement(15450..16208)
                     /locus_tag="ONT67_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCR2354638.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ONT67_000880"
     CDS             complement(16226..17044)
                     /locus_tag="ONT67_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2354639.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT67_000881"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ONT67_000881"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR2354640.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT67_000882"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ONT67_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR2354641.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT67_000883"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ONT67_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR2354642.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT67_000884"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ONT67_000884"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR2354643.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT67_000885"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ONT67_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCR2354644.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ONT67_000886"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ONT67_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR2354645.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT67_000887"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ONT67_000887"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR2354646.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT67_000888"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ONT67_000888"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR2354647.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="ONT67_000889"
     CDS             27086..27385
                     /locus_tag="ONT67_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR2354648.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ONT67_000890"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ONT67_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2354649.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="ONT67_000891"
     CDS             27973..28209
                     /locus_tag="ONT67_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR2354650.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ONT67_000892"
     CDS             28352..30325
                     /locus_tag="ONT67_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCR2354651.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ONT67_000893"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ONT67_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR2354652.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT67_000894"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ONT67_000894"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR2354653.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ONT67_000895"
     CDS             33204..34496
                     /locus_tag="ONT67_000895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR2354654.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="ONT67_000896"
     CDS             35232..36095
                     /locus_tag="ONT67_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2354655.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="ONT67_000897"
     CDS             complement(36224..37846)
                     /locus_tag="ONT67_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2354656.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="ONT67_000898"
     CDS             38095..38616
                     /locus_tag="ONT67_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2354657.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT67_000899"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="ONT67_000899"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCR2354658.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="ONT67_000900"
     CDS             40697..41515
                     /locus_tag="ONT67_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCR2354659.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="ONT67_000901"
     CDS             complement(41618..41815)
                     /locus_tag="ONT67_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354660.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="ONT67_000902"
     CDS             41984..42562
                     /locus_tag="ONT67_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2354661.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42657..42794
                     /locus_tag="ONT67_000903"
     CDS             42657..42794
                     /locus_tag="ONT67_000903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354662.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43607..44110)
                     /locus_tag="ONT67_000904"
     CDS             complement(43607..44110)
                     /locus_tag="ONT67_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCR2354663.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="ONT67_000905"
     CDS             44469..45494
                     /locus_tag="ONT67_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2354664.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="ONT67_000906"
     CDS             45817..46695
                     /locus_tag="ONT67_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCR2354665.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="ONT67_000907"
     CDS             47171..47287
                     /locus_tag="ONT67_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2354666.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="ONT67_000908"
     CDS             48215..48553
                     /locus_tag="ONT67_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354667.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="ONT67_000909"
     CDS             48647..48877
                     /locus_tag="ONT67_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354668.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="ONT67_000910"
     CDS             49277..49648
                     /locus_tag="ONT67_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2354669.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="ONT67_000911"
     CDS             complement(49922..50614)
                     /locus_tag="ONT67_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2354670.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="ONT67_000912"
     CDS             complement(50617..51402)
                     /locus_tag="ONT67_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2354671.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="ONT67_000913"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="ONT67_000913"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCR2354672.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="ONT67_000914"
     CDS             53064..53381
                     /locus_tag="ONT67_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCR2354673.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="ONT67_000915"
     CDS             53394..55067
                     /locus_tag="ONT67_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCR2354674.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="ONT67_000916"
     CDS             55081..56712
                     /locus_tag="ONT67_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCR2354675.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="ONT67_000917"
     CDS             57584..58774
                     /locus_tag="ONT67_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HCR2354676.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
BASE COUNT        18156 a        10872 c        11742 g        18191 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaaa c
//
LOCUS       DAJSBM010000013        56761 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJSBM010000013 DAJSBM010000000
VERSION     DAJSBM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.013"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            446..832
                     /locus_tag="ONT67_000918"
     CDS             446..832
                     /locus_tag="ONT67_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2354677.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            926..1117
                     /locus_tag="ONT67_000919"
     CDS             926..1117
                     /locus_tag="ONT67_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354678.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1631..1963
                     /locus_tag="ONT67_000920"
     CDS             1631..1963
                     /locus_tag="ONT67_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2354679.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2259..2414
                     /locus_tag="ONT67_000921"
     CDS             2259..2414
                     /locus_tag="ONT67_000921"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCR2354680.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2690..4030
                     /locus_tag="ONT67_000922"
                     /pseudo
     CDS             2690..4030
                     /locus_tag="ONT67_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4271..5887)
                     /locus_tag="ONT67_000923"
     CDS             complement(4271..5887)
                     /locus_tag="ONT67_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2354681.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6005..6223
                     /locus_tag="ONT67_000924"
     CDS             6005..6223
                     /locus_tag="ONT67_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354682.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6418..6879
                     /locus_tag="ONT67_000925"
     CDS             6418..6879
                     /locus_tag="ONT67_000925"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2354683.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7072..7311)
                     /locus_tag="ONT67_000926"
     CDS             complement(7072..7311)
                     /locus_tag="ONT67_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354684.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7335..8858)
                     /locus_tag="ONT67_000927"
     CDS             complement(7335..8858)
                     /locus_tag="ONT67_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCR2354685.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8876..8998)
                     /locus_tag="ONT67_000928"
     CDS             complement(8876..8998)
                     /locus_tag="ONT67_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCR2354686.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9028..10011)
                     /locus_tag="ONT67_000929"
     CDS             complement(9028..10011)
                     /locus_tag="ONT67_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCR2354687.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10035..10184)
                     /gene="rpmG"
                     /locus_tag="ONT67_000930"
     CDS             complement(10035..10184)
                     /gene="rpmG"
                     /locus_tag="ONT67_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2354688.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10205..10582)
                     /locus_tag="ONT67_000931"
     CDS             complement(10205..10582)
                     /locus_tag="ONT67_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354689.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10614..10793)
                     /gene="rpmF"
                     /locus_tag="ONT67_000932"
     CDS             complement(10614..10793)
                     /gene="rpmF"
                     /locus_tag="ONT67_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2354690.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10808..11077)
                     /gene="rpsN"
                     /locus_tag="ONT67_000933"
     CDS             complement(10808..11077)
                     /gene="rpsN"
                     /locus_tag="ONT67_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR2354691.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11600..13366)
                     /locus_tag="ONT67_000934"
     CDS             complement(11600..13366)
                     /locus_tag="ONT67_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354692.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13326..15080)
                     /locus_tag="ONT67_000935"
     CDS             complement(13326..15080)
                     /locus_tag="ONT67_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354693.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15190..15759)
                     /locus_tag="ONT67_000936"
     CDS             complement(15190..15759)
                     /locus_tag="ONT67_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCR2354694.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15777..17192)
                     /locus_tag="ONT67_000937"
     CDS             complement(15777..17192)
                     /locus_tag="ONT67_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2354695.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17203..17871)
                     /locus_tag="ONT67_000938"
     CDS             complement(17203..17871)
                     /locus_tag="ONT67_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR2354696.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18001..18585)
                     /locus_tag="ONT67_000939"
     CDS             complement(18001..18585)
                     /locus_tag="ONT67_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2354697.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18916..19149)
                     /locus_tag="ONT67_000940"
     CDS             complement(18916..19149)
                     /locus_tag="ONT67_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCR2354698.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19164..20114)
                     /locus_tag="ONT67_000941"
     CDS             complement(19164..20114)
                     /locus_tag="ONT67_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2354699.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20111..20953)
                     /locus_tag="ONT67_000942"
     CDS             complement(20111..20953)
                     /locus_tag="ONT67_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2354700.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(20955..21674)
                     /locus_tag="ONT67_000943"
     CDS             complement(20955..21674)
                     /locus_tag="ONT67_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2354701.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22366..22524
                     /locus_tag="ONT67_000944"
     CDS             22366..22524
                     /locus_tag="ONT67_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354702.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            22596..23822
                     /locus_tag="ONT67_000945"
     CDS             22596..23822
                     /locus_tag="ONT67_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2354703.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(23910..24302)
                     /gene="rpsI"
                     /locus_tag="ONT67_000946"
     CDS             complement(23910..24302)
                     /gene="rpsI"
                     /locus_tag="ONT67_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR2354704.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24318..24761)
                     /gene="rplM"
                     /locus_tag="ONT67_000947"
     CDS             complement(24318..24761)
                     /gene="rplM"
                     /locus_tag="ONT67_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR2354705.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25280..25747)
                     /locus_tag="ONT67_000948"
     CDS             complement(25280..25747)
                     /locus_tag="ONT67_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCR2354706.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25867..27405)
                     /locus_tag="ONT67_000949"
     CDS             complement(25867..27405)
                     /locus_tag="ONT67_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR2354707.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27485..28171
                     /locus_tag="ONT67_000950"
     CDS             27485..28171
                     /locus_tag="ONT67_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR2354708.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28372..28899)
                     /gene="msrA"
                     /locus_tag="ONT67_000951"
     CDS             complement(28372..28899)
                     /gene="msrA"
                     /locus_tag="ONT67_000951"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2354709.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(28983..29660)
                     /gene="pgmB"
                     /locus_tag="ONT67_000952"
     CDS             complement(28983..29660)
                     /gene="pgmB"
                     /locus_tag="ONT67_000952"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR2354710.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29657..30787)
                     /locus_tag="ONT67_000953"
     CDS             complement(29657..30787)
                     /locus_tag="ONT67_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2354711.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30787..32970)
                     /locus_tag="ONT67_000954"
     CDS             complement(30787..32970)
                     /locus_tag="ONT67_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCR2354712.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32967..33992)
                     /locus_tag="ONT67_000955"
     CDS             complement(32967..33992)
                     /locus_tag="ONT67_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2354713.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34037..34864)
                     /locus_tag="ONT67_000956"
     CDS             complement(34037..34864)
                     /locus_tag="ONT67_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2354714.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34861..35928)
                     /locus_tag="ONT67_000957"
     CDS             complement(34861..35928)
                     /locus_tag="ONT67_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR2354715.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35954..36790)
                     /locus_tag="ONT67_000958"
     CDS             complement(35954..36790)
                     /locus_tag="ONT67_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2354716.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36787..37656)
                     /locus_tag="ONT67_000959"
     CDS             complement(36787..37656)
                     /locus_tag="ONT67_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354717.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37726..39009)
                     /locus_tag="ONT67_000960"
     CDS             complement(37726..39009)
                     /locus_tag="ONT67_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2354718.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39023..40708)
                     /locus_tag="ONT67_000961"
     CDS             complement(39023..40708)
                     /locus_tag="ONT67_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR2354719.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            40983..42008
                     /locus_tag="ONT67_000962"
     CDS             40983..42008
                     /locus_tag="ONT67_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2354720.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42603..46277)
                     /gene="rpoC"
                     /locus_tag="ONT67_000963"
     CDS             complement(42603..46277)
                     /gene="rpoC"
                     /locus_tag="ONT67_000963"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR2354721.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46335..49952)
                     /gene="rpoB"
                     /locus_tag="ONT67_000964"
     CDS             complement(46335..49952)
                     /gene="rpoB"
                     /locus_tag="ONT67_000964"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR2354722.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50680..51648
                     /locus_tag="ONT67_000965"
     CDS             50680..51648
                     /locus_tag="ONT67_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2354723.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51666..52571
                     /locus_tag="ONT67_000966"
     CDS             51666..52571
                     /locus_tag="ONT67_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354724.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52568..53374
                     /locus_tag="ONT67_000967"
     CDS             52568..53374
                     /locus_tag="ONT67_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2354725.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53510..54499
                     /locus_tag="ONT67_000968"
     CDS             53510..54499
                     /locus_tag="ONT67_000968"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCR2354726.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54816..55340)
                     /locus_tag="ONT67_000969"
     CDS             complement(54816..55340)
                     /locus_tag="ONT67_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCR2354727.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55627..>56761
                     /gene="scm"
                     /locus_tag="ONT67_000970"
     CDS             55627..>56761
                     /gene="scm"
                     /locus_tag="ONT67_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2354728.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSD"
BASE COUNT        17174 a        11474 c         9669 g        18444 t
ORIGIN      
        1 atacagataa taggaatttt tcgtttctaa atattccaat ttgggaatcc aataaaaatt
       61 ttcgttcaaa tacgtgtcta ttcgaactct tttctagttg ttaattatga agggggaaga
      121 aataaaaaat acaatctata cacgaataag aatattatcc aaaaggaggt tttagaaaag
      181 atttattata cgtaaaaagc tcaagacatt ttcttagcca taaggacatg tttgatattt
      241 aagaagatat atgaattttc ttttctaagt ataaagataa ctatccatta tcaactaaaa
      301 aaacattttt gaaaataaac atcagcagtc ctatcaatgc atgtgagctt atagaagtta
      361 ttcgcagcgc agcataattg cattagcttc ttttcgtgtt ttttgcctct tttcccttta
      421 aatcttaatg gaggaggtgt gcaatatgtc agataacgac gaacgcgtaa atgcccagtt
      481 gattcgattc tttgaaaagg tcattttgaa caccgctaat acttattata aaaaacaaga
      541 agaaattcaa aaacatgaac aacttgatca actcccacca tatttgttta ctaaagatac
      601 tataaaattt aaacaaccga ttaatgtttt aaatgtgact ctccttgttg aagatataaa
      661 attagcaaaa atccttcctt tgttgaaaga acctgaacaa atttttctta tcgaaaaatt
      721 tatctttgat aaaactgata aagaaattgg aaaggttctg gggattacaa gacagggtgc
      781 gacaaattta aaacatcgac tttacaaaaa aattcaaaaa atgctggttt aatatctgca
      841 taataactaa ttttgtatgc ttctttgctc gttctcctcc ttcccaaaga aatagattat
      901 aagtccatgg gaagaaggta gatttatgaa ttctttaaaa gaagatttca ttttagcaaa
      961 ggctggaaat gaagaagctg ttgaggcaat tctcaaaaga tttagctctt tgattcataa
     1021 gcaatcctgg agaactggaa aatatgatca agattgttat caagaatgta tgcttgcaat
     1081 atatctagct atctcgaaat tcgaaattaa agaataactc aactaaggga gtctgtgaca
     1141 taagtaaaaa gatagtttca aatttcgaac aataaatttg aaactatctt ctttataatt
     1201 ataaagtatc gattttacag cgttctataa tgattcatag aatctaaaac gaatgatttt
     1261 aattatcaac gttttttaat tcgtttttag cttaaaaata acttttgtca cagtctcact
     1321 cctaaaatta ctttatttac gtatgaagtg ggcaggaggt cgaatccaga actgggctat
     1381 ggtacttcaa cgaggacttc tcctctagaa ttgaaaagca tgcgatttac cttccgtaac
     1441 gacggtagat ttattcaact atgtgtccaa tcaaaatttg tctgatttca aaaatagtca
     1501 agagaatttt ggttgttaaa aactcttatc tatcaacttc cacagtttag ttgacaaacc
     1561 ctgatactaa gttgggctat gatacacttt tcttgactat cagttacgtt accggaagga
     1621 atgaactcta ttgagaaaaa catatgatcg taaatttaaa ctaaagattt cacaggatat
     1681 ccttgagaaa aaagtaacta ccaagaaaat tgctgaagaa tacaatatct ctcgtccaac
     1741 aatttcccga tgggtttcag aatatcgtcg atacgggaaa aatgcttttg ctggacaagg
     1801 aaaaagatta cctgataaag cagatttcta cattcttgaa caggaaaata aacgactaaa
     1861 agaggaagac gagtgttcta cgaacacaaa gatcgatacg gatctccagg gatcactcaa
     1921 aaactttacg atgaaggaat cgaaatcaat aaacgtgttg tagctgttct tatgcgtgaa
     1981 atgaatttat gcacaagagg atttcatcga cgcaaatctt ctcatggtag agaaaaaaca
     2041 atcaaagaaa tggtaaaaaa atgttgaatc gataatttga acagaaccaa attgatgctg
     2101 tttgggtgat ggatattact tacattccat gtaatgacgg tcgcctctat ttgtccacct
     2161 atattgactt agcaactcgt attctacgtt actatatggt agattcacac atgaaaaaag
     2221 agattgtcgt ccgtccatta caaatttatt aaggagaatt gcctacggta attcattccg
     2281 atagaggtag ccagcatcgg tcttttattt atcaagagct gttgaacgac caccacatca
     2341 cacattcagt gagccaaact gggactcctg ttgacaatgc agtaattgaa gcgtttcatc
     2401 gatcaatcaa atgagaatta atcgactcca acaaatataa aagtaaaata gagatgaagg
     2461 tgttaatcca agattattta gaagactact acccaaacaa aagaatccat acaaaattta
     2521 tgatgactcc ttgacaattt gaagcgaaga aaataaggga tctataatat ctagtgttgg
     2581 tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttaa
     2641 aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc
     2701 agagtttacc aaagagttac tagatattct agacctaaat cttatctttc atgaagatgc
     2761 ctttcgcaaa gagcgaatca atgacgaaac atgttttgtt tttgatggca cattgaccta
     2821 tcaaccggag gaatgctttc atcgccacta tcaaaatgaa caaacgatca ttaaataggg
     2881 ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca ttctgcggat
     2941 taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt
     3001 tagtgatcgt cgctgctcca ttgctcgcag agtcaaacaa gccattcttg agctattaag
     3061 cgaacctctt tctatgtctt tgatcgcacg tatgaagcac atttctccga ccactgtcat
     3121 tcgaatactt cgcagccttc ggcctaaaac catttctttg agccagctgt taccagaagt
     3181 ggtgtgtttc gatgaattca aatcagtgaa aaatgtctcg ggagccatga gctttgtcat
     3241 gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt
     3301 acgcgactat ttcttgcgct accctataaa agtccgtgaa caagtgcgtc tagtggtttc
     3361 agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt
     3421 cgccgatcgc tttcatatta gccaacacat tggtcgggct tttaccaacc accgaattca
     3481 agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggaa
     3541 actacttcaa aagaatgcgt gggaattgaa aggtcagcat cgctactggc gtccgtcatt
     3601 tcgagatcat ctgacagaag cagagatagt cgatcggcta ttatcttatg atgattcctt
     3661 aaagcgaggc tacgaagtct accaggattt tctttccgtt atcagaagac aagatgttcc
     3721 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact accaaccagt
     3781 gttcacgacc ttcaagaaat accaaacaga aatcaaacga gcgttgcgcg ttccctattc
     3841 caacggccct atagaatgtt tgaataacca tatcaaagta ctaaaacgaa ttgcctatgg
     3901 ctatagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa aatattttaa
     3961 gaaaactaag aatactaccc aactgactaa agccagaaca acaacacggc tagacaagat
     4021 agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt
     4081 gacgaagagc caaaataagt aatctaaccg actgttaact aaaccattgc aggtcccgtt
     4141 aaagatactt gtgcctgttg tcaccgcttc tttttcttgt ccacaagcgc ttaacacact
     4201 taataaagcc cctgctatta ttaataataa tatctttttc ttattaaaaa cctcctcatc
     4261 tatttccaga ttagtaaatg tagtaatctg tgctttcttt tttctcaata ctaataacta
     4321 acttatgggg taaacaaata ctcgtttgac cgggttttga gatccatcca gttctaaccg
     4381 cgatttgatc aggactatta tcttctttaa ctcgaattct tccatcttta atctcaacaa
     4441 tattatattg tcccttcgca ggatgataat tcacgagttt atgattgatc ttatccaaat
     4501 cgaagcgatc catttccttg ccatcaattc gaacgatggc atatttgata gcatcttcag
     4561 ttgtttcatt ctccttttct atagagaata caaataaggg aataaaagag gtaataatca
     4621 aaatgccaat aattatgaca tcgcctatct tgacaccagc ttttcttaaa aatttcatta
     4681 acgatatctg gctaaacata ttagactttt tccatggaaa tggtgaaatt tttcggaaat
     4741 tatctgcatt aagagaatca gttaaacaac cctcaagttg ttccttaata cattgttgat
     4801 ccatcttcat accaataaaa actagtttat taatgcgttc accaaacaac ggatgccagg
     4861 cttccttcaa ttcgggattt ttttgtataa tcatttgaca ctcaatttta ggtaaactag
     4921 ctacccaata atttaatgat tcaatacttt tatagctacc cgatgtttca aagatcgtcg
     4981 aaaaatcact atgttgggca aaccaaagaa acccttttgc tcttgtaata ttgccaggaa
     5041 aatttttatc cgtaagatgg gcaaattttt gtgcgtcaag gagtaaccga tttcgataga
     5101 taaaacttga gataccatat tcttctgttt caggaatatg attttgatag ccaagctcta
     5161 actctttgaa ccagcccact gaataggaag tctcatctat attaaaaaga tttgtattta
     5221 gaattttcga taagtctact tcacctttaa ttgtcctaat tattttggca ttggactgta
     5281 acttagcaat tagaccgtta attttgtaaa tcgtcttggt tgacactaag tcactattat
     5341 ttaaaagtaa tatgttacaa cactcgaatt gatcaattaa caagccacga atatcctgtt
     5401 cttcttcaac tacattacct tcagcttttc tttcctgaat cttttcaccc gtttcgaaat
     5461 tatcccaaaa acgttttaca tccactacta taataatggc accaatctct aatcgttggg
     5521 tcaaatcaat gtctaaaggg tttttagcta atataattgc ttgaataatt ggaattggtt
     5581 tgctgattcc tgaagcttca ataacaattt gattcaagtc agaaaattgc actaattgat
     5641 ctagttcaac aatcaaatct tcgcgtaatg tacaacaaat gcatccattc ggcatcgaaa
     5701 caagtttttc tccagttctt ttaaaacctt tcttgtcgac taatacccca tcaacattta
     5761 cttcccctat gtcatttaca atgacagcaa tttcctttcc gtgatcaccg tacagaatat
     5821 gatttagaag tatcgttttt cctgaaccaa gatagccagt taagattgta ataggtattt
     5881 ttttcatagg gaacctcttt ctataccttg aatttacact ttaagtgcaa cacataaaaa
     5941 tgagtcataa tcgatatcca actagtataa gtttgtcgag aacactccta gaaggaaaat
     6001 aattatgagc cagtatcgtc atcttactat actcgaacga gaaagaatct ttctgcttca
     6061 cgaagaagga ttttctatta gtataattgg caacgacatt cagcatagcc cgtttactat
     6121 tttacatgaa ctgagaagaa acaagaaaaa aactattcac cttccaaagc ggaaaaaaat
     6181 tatgcttatc gaagaaaaaa atgcggacgt tatcacattc taaatgatcc gtgagtttgg
     6241 tcaattgttc gtcatttatt tgtcgatttt cagtggtcgc cagaggaaat ctcgaatcgt
     6301 ttgaagcatg aacatatgga cttttcaatt agttttatca cgatctaacg tgcaatttgc
     6361 aatgatcttt tcgatacaga acccctctca cacggcaata aaggcctgat ccgttcattg
     6421 caccatcgag gaaaaacaag acatacaaaa aatcatgtcg agtctcgtgg aaagatttgc
     6481 cttactcatt cgatccatgg ccgtccaaaa tccgcagatg atagaagtga ttttggtcat
     6541 tggaaagccg atattgtagc tggcaaaact ggtggtactt gtttagtgac tttgacagac
     6601 agaaaatcat gcttcctttt agcaggaaag aaggtcccca gaaagaaagc cgcttatgtg
     6661 agcgagaaaa tgattgaact tttaagcagt attccaaatg aggcggtcaa aacgatcact
     6721 ccagatagag gaaaagaatt ctccaatcat ttcgcagtta cagaagcatt gaataatatg
     6781 cccttttatt ttccagatcc tcatgccctt ggcaacaatg aacaaattaa aatacgaata
     6841 gactcattcg agaatacttg ccaaaatcga aagagatagc tacggtagat aactcaacga
     6901 taaatcagat gattgaaaaa ctgaataaca gacctagaaa gtgtttagat tggagaacac
     6961 cttatgaagt cttctttgat aaagtgttgc gcttgatttg acaattcaag acataataaa
     7021 aagcagatca aatccaaata agggaaaaat gatctactct ggtattatca attaattggc
     7081 caagctatta tgccgttttg ctaaacgtct acttcttcca gtattgacct tgataaaaca
     7141 acaatcagga tcccgtttta atactctatg aactgtgtca atactaacat gtaaagttgc
     7201 agcaatatca gctaatgtac tcccatttaa tctcatttgc tttgcaaatc gataacgccg
     7261 ataagcatca atattcttgg aatccataga caataatact tttcttacca tgtatttcct
     7321 ccaaaaattg aattttaatg agccatcatt tcttgagcaa tttcatgttt agacaatgaa
     7381 gctggataga aagtaggcca attatgcaat tcattataga gtttttcttg actttctcca
     7441 ccaaagaaaa tatggaaatg ctctgcctta cttggtgcaa ttccatgatc actaaattga
     7501 atatatttaa aacgaccagc acctgcatca tcagtttcaa aattaaaacg aacaccacgg
     7561 tttccttttt cataattcaa aattttgtat ccaacatatt tgtatgtgta ttgatgatgt
     7621 tcaccgttga ctacaaaatc aatggtgttg tctttaatgt tgatagtatc aatatctgtc
     7681 ttatagccag tagtataata atctttgtac tcggcagctg tcatgtcttt attgattttt
     7741 gctttgtaat cgaaaacttc atccaaaatt ccatctttta acaacggata aacagattgc
     7801 cattcgcctg tataatcact caattcgcga tccttcacat cggcatcttt aaagtaccca
     7861 ttagcaactg tcttttcatc cttcgcttct ttttctggaa ctacctcttc accagcaacc
     7921 atagttgttt tttctaacgc ttttagattg tcttccatta ctgaaacata atcttcacca
     7981 ttatccattt gttcttttgt aagactttca agaggattca gtacttccaa ttttatccca
     8041 gcttcgttgg ccaaagtttt tgcaatttta tcattagcat tcttttcaaa gtaaatataa
     8101 ttgatcttat tttttttaac atactctttt aattcagcta atcgaccaga agatggctct
     8161 tcttcaggac ttagtccagc aataggaact tgtttcaatc cataatccaa agctaaatat
     8221 ccaaaggctg cgtgttgcgt cacaaaactt ttttgtttcg cttctttaag actagtggta
     8281 tagtcagcat ccaaccgttt cagtttagtc aaatactttt cagcatttgt ctcaaatact
     8341 tttgcttttt tcggatacaa tttaactaac tgatccttga tatttgaaac ttccttaatt
     8401 gccattttgg gtgaaaccca cgtatgagga tctagttcat gatggtgatc ttcttctccg
     8461 tgatcatgat cttcttcttc actacctggc aataaaatca tgtcttttgt tccttctact
     8521 acattaggcc cagctttctt ccaactttct ttagcttttg gtacccatga ttccatgttt
     8581 tcgttatgat atacaaaaac atcagcatca tgagcttttg ccatatcctt agctgagggc
     8641 tcatagtcat gaggttctga tcccgctgga accattaaat ccacgttcgc ttcatcgccg
     8701 acgatgtttt tggtgaaatc atacattgga tagaatgttg ttacaatttc taattttcca
     8761 tctttatcat tcgatacttt tgcatcatta gtgctggagc aagctcccaa taatcctaga
     8821 gctgctatcc caataagtcc tacactgatt ttcttcattc tttttccttc cttaattatg
     8881 ctaatttctt cattttattt cgtttatgtt ctttgataat ttttaatgcc ctttttctag
     8941 ttttaatatt cgggctttta agtcgtcgat atgcactaga tatatccgtt ttctccatta
     9001 gaaattaccc ccaatcacta cttatcatta tttgctttcc gccagtaatt tatcaaaact
     9061 ctgtttgatc ctttcttcat ccaacatttt tccgattaga ataatagtgg tttggtcttt
     9121 ttgtcgagtg gtgtattgaa aattaacctg ttcgtttacc tcttgcatgg caactataaa
     9181 atcctgctct tgtaaagcaa tcaatccttt aaaacgatac aggctccctt ggttggttaa
     9241 aatcagccag tcaatccatt ggagtaaaaa atcttttttt atgtcttgat ctgaagttaa
     9301 gagaattgat ttaaactcat gtacttgatg gtgttgatga tagtatttgc cattatagtg
     9361 attttctgat gaatcacttt caaaaactaa gggatgatta aattttttca aattataaaa
     9421 atcctttttt tgaatcgttt cactatgtgt gaacattcga atatcggata aagggttaat
     9481 acttttgatt tctctctgta atttttttag tgcagataaa ttcaagtttt gttttctaga
     9541 cacaaaaatg cgatctgcca tgacaagttg cttaagggct tgtggttctc tttcaataag
     9601 ttcaaaactg tcactatcaa taacagtcaa taaactatcg atatatactc ttcctcttag
     9661 ttgaggcgct gttaaaagtg tttgaatgat tggttgggga tctgctatac ctgtagtttc
     9721 aatgatgatt tgtgaaattg gttgttgatg ttcttctatt gtttccagta aagaaatcaa
     9781 tattgctaat aaatcactgc gaagactgca acacatacat ccattattta gttgtaacac
     9841 ccgttcctcg ctatgaacta ataatttgtg atcaatccca acttcaccaa actcattttc
     9901 aattataata atttcttccc tggggtaggg tgagacttgc aaggcttgat tgattagcgt
     9961 tgtttttcca gcccctaaaa acccagaaac gattgtaact ggtatgccca ttttgtcacc
    10021 tttttaccac ttagctattt cacttcttta aaaattgccc gtctgcgcaa ttttggcgaa
    10081 tactttttta attctaaccg ttcagggtta ttccgtttat ttttgctggt gagatacaga
    10141 cgttctccag tttcgacaca ttccaaaata atattttgac gcataaatct tcctccttca
    10201 atacttatcg atcttcaaga tatgcttgaa tttgttgtgc atcttcaaaa gttaactcgc
    10261 cagattccaa ttgtgaaact aaattttctt tcatatcaac aggtatctcc caataaccat
    10321 cgatacattg aaataactcc tcgtcatact cttcaaaaat tggatagatt cttaaaaaat
    10381 catcaaatga taagccttgg tcctcatagt aatctttgac taatccattt tcaattgcct
    10441 gttgggtcgg taaacccgtt tcagtaataa ttcctctctc taccatcgaa cgataaaaag
    10501 tgcatttatc tagcgattgc tgggtcgttt caagataata ctttaatcct tcttcaacta
    10561 aaggtgggta aataatttcc attttgattc cctcctaaaa gttatatatt aaactatttt
    10621 gaagctcctt taattacttt ctttccatta tagtaccctt ttaaagatac atgatggcta
    10681 cgtctataat caccattcgc ttcgtcaaat gaaatttctg gttttactac agtttgatga
    10741 gttcttctta accgtttctt cgccttagat gtttttcttg ctggtactgc catgatattt
    10801 cctcctttta ccaacttgct ttttttactc caggaatcaa cccttgatgg gcaagttctc
    10861 taaacttaat acgggacata ccgaactttc gcatataccc tcttggacga ccatccgtct
    10921 gatcacgaag cctcagacga ttcggatttg aatctcttgg caatttagcg agagcggtgc
    10981 gatctcctgc ggcttttaat tcctgacgtt tatctgcata cttttcaatc atcgccattt
    11041 gtttctttgc tttagcaatt ttcgactttt ttgccatata cctttatact ccttttattt
    11101 tgatttagac acctcaaatc gtaatcgtta cgttttgaca ctgatacttt accaacaaga
    11161 gttgctcttg tcaacaataa ataacatgaa ataaaactca tatgaataga taactatttg
    11221 aaaacgaatt tatcggtaca tagagataat caaatcattt aaaagaatat gcagtttagg
    11281 ctctgaaaaa taagaattca cttttattag tccaatcaaa cacaaatacc gttttaagta
    11341 gcttttcctc tacttaaaac ggcaatatga aagaaactat aactttttat tcaatctttt
    11401 atgtttaact ggtttatcgc caatttttta aaactttgat tattccttat attataagaa
    11461 tagacattaa gtgaactaat tcaatcatac tgattctttc gtgtattcaa aattagatgc
    11521 tgtttaataa aattattctt tttgaaaaag aattataact atatcccaaa aattgtaggt
    11581 taatttaaag cctatttcat catcgtgatt gagcttcttt ttcatagacg aatttctcag
    11641 atagtttagt tttctttatg agatttttgt atatttctga ccttcgatac aactcattat
    11701 gagtaccttg atcaactagt tttccttgat ccatcactac aattttattt accttttcaa
    11761 tagacttcaa gcgatgcgat ataattagta ctgtcttatc ttgtattaaa cgatttaaac
    11821 tttcctgaat acgtttttca ttttccgcat ctaaactggc agtaatttca tctaaaatta
    11881 aaattggcgc atttttcaaa aaagcccgtg ctatggatag gcgttgtcgc tctcctccag
    11941 aaagtgaact tccgttttct ccgaccaatg tatgataacc gtcaggtagt ttttcaataa
    12001 agtcttcgca gttagctaaa cgagctgctt ttttcacttc ctcatctgac gcttgactat
    12061 ttccaatacg tatattttcc aaaatacttg tattaaacaa agttacctct tgaaaaacaa
    12121 tagcgatttt gctaaacaaa gaagctggag atacacgttt aatatcatac ccatcaatta
    12181 aaatacagcc ttcatcataa tcatacagtc tagacactaa tttcaagata ctagttttcc
    12241 cggaaccact agcacctact aacgcagtca cttctccttg tttagccgta aaagagatat
    12301 gatctaaaat tggggtattg ttatcatatg agaaagagac atctcgcaac tcaatgtcaa
    12361 aagatttcag aggactatct gatccctctt ggatactggt ttccttcata gctctaattc
    12421 gctgtatttt aggagctaaa taaaatattt caagcactgc ttctttcata gaatcgaagg
    12481 aatcctttat ttttattgcg gctagtaagt aaccaacgac gtagagtata gtcacttcac
    12541 ccgttaacag taagtgaaca cctactagaa tgactactgc taaactaaca tatgagaaaa
    12601 tagaggataa ggccatgact gaaaaagtac ttaattctac ctttaaatga atcctttcac
    12661 tctcttccat ttttttgaaa agtctgtctt gaactttttt agatagatta aagctattaa
    12721 tctcttgctg caattctata gtttcttgaa attcttccga gttttctctc aaagtatcgt
    12781 aatatttagt attggctttc gtttgggatt tctttgataa caagattaag acaaaactaa
    12841 ataacgttgg caaaataaca gctaatccca ttttgacatt accaaccaaa agcatcactg
    12901 aaataaaagg gaaaaacaga gccataccgc cggctttagg tattgcatga ctcatcgcat
    12961 gctcaatacc ttcaacgtca gacatgatag tttgagataa atctgataaa ttatgttttg
    13021 aaaaatagga taatggtaaa tttgacaaat cgtctgcaat ttgcactctt aaatgcgcac
    13081 tttctttata agtactatta tataagtttt catactccct atctaacaac caatacatga
    13141 aaagcaaagt aacaaacgat acggcaaaat aaagccaacg gggctttgta ttttctaaaa
    13201 ctaattcgtc tatcagcatc attataatga atgctggtac catattaatg cagtatacaa
    13261 aaaaactaga aatagaagct ttagttaaag cttctgcgcc ctgatcactt aaagcaaatt
    13321 tgtttttata aaaacttttc attgtcaagc ctccaatcat ttgcattctc ataattagtg
    13381 accaattgtt tatacacttg atgtttagcc attaattcat tatgatttcc acgttctact
    13441 attttccctt ctgacaagac aagaatttca tctacagatt taatgctgga taatcgatga
    13501 gctatcataa taactgtttt ttctttcatc agatgttgaa aagccttttg tagttcatac
    13561 tcattatctg gatcaataga tgcactggct tcatccataa taacaatctt tgcatctttt
    13621 aaaattgctc tggcaatagc tatacgttgt ttttctcctc ctgacagata aactccttta
    13681 ctaccaatta aggtatgctc tctttcggga aatttttcta aaatcgattc gcaaccggct
    13741 aatcttagcg cgtcaaatac tttacttgct ggcgcttgag gatttgctat agaaacgtta
    13801 tcgtaaatac tctgattgaa tagtttcgta tcttgaaaga caaaagcaat tgtgttaata
    13861 attgtgtctt ttgaatagtg atttaaaggc ttaccaccaa ttttaatctg tcccttatcc
    13921 acattgtaga aacctgaaat tagtttagct atcgtactct ttccgcttcc tgattttcct
    13981 actaatgcat aaattctttt ttgttctaaa actaagttga aattttctaa aacacactct
    14041 tctttgtatg aaaagctgac attttcaaat gcaatgtcac aatttttaat ctgtgtctcg
    14101 ttcccatatt ttagttggtc ttgcttcatt tctaaataaa gggagtctag tgtttcgact
    14161 gcatatcttg cttcaaacaa gtgcatactt acatacatta ttctcatgaa agaaacaaaa
    14221 ataattccac ttaaaaaaaa caacatcaac aagtcaacag ctaatatctt gggagtggta
    14281 agagaagtta taaaaaaggt tataggaact atcaaaatgc aaactatgcc aaaaaatagc
    14341 cattgaaaaa gaacaaaagg tgttttccca ctcaaagaat acttatatgc aaacttagca
    14401 taatcgttaa ttgctttatt caaggcttta aatgatgtta ctttagcacc aaatatcttt
    14461 ataacttgaa ttccgcggat atattctacc gtttcagagc ttaacacatc taacgattct
    14521 tgatattttt gaataaaatt tgtattctct gtcattttct ttaacaagaa tacaccaatt
    14581 actaacatgg cgattagaac caatccaatt ttaaagttaa tgaaaaagcc taaggcaact
    14641 gataaaacag gtgttactat ggcttgagca ctatctggaa tcaagtgagc aattgccata
    14701 tgtgttttag ccgcgttgtc atcaattgtc tttcgaatat atcccgaaga atttaaatca
    14761 aaaaaacgaa aacttgcttc tgttaaaccg ctaattccct tttttctcaa attagtctct
    14821 aagcgaaacc caaggatatg agaaaataag ccagaaaaaa tataaaataa agctcctaaa
    14881 gttaaccaag caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata
    14941 attagagact gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa
    15001 aaatcagaaa ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat
    15061 aattttttgt atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt
    15121 acaactgtta atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta
    15181 aactacgttc tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat
    15241 agataaactt gctacaacta acactgaaat gattccccat atacctaccg catagtggat
    15301 cattgcatcc attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc
    15361 tgtaaagaac catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca
    15421 agacgcaact tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa
    15481 gatcgccata ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa
    15541 aaataacaat aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct
    15601 aatataaaca aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc
    15661 aaacgcccca ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac
    15721 tgtagctaaa ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat
    15781 ttcgttatgt cattaaaaat ctgttctatc aaagttgaat cttctactct aagttttata
    15841 tcttttttta ttccaccatt ttttaaataa ataattctat tgcatgtttt ataaagaaat
    15901 tccggatcat gagtagctat aactactgca cccttttctt ttaagtcttt aataaggtca
    15961 gcaatccttt ccatattaat ataatccaac ccacttgtag gttcatcaaa tagaaataaa
    16021 ttagtttttg acaatgaagc tatagaaacc aataatcttt gtttttctcc accggataaa
    16081 tcaaacggta attgatcttt cattttataa agttccattt tttttaaaat tttttcttgc
    16141 tgatctttac taactatttc attccccagg gctaactcgc tcctcactga ttctgtgaaa
    16201 aattggtaat ccatttcttg cattactaaa aacggcgatg acaaattttt tttattttcg
    16261 attatctttc ctttggtagg gcgaataatc ttcatgagta ttcttagtaa ggttgttttc
    16321 cccacaccgt ttttccctac caaaccaata acatctccaa ctttgacatc aaaatttatg
    16381 tcttttagaa tattttcata agtaatttct tcaactttta atacatctgc acttaccttt
    16441 tcgatatagg gaacttcaat cgaactatta aaaatgtcaa atttccttaa tgcgttattg
    16501 ttttcagttt ttaacaactg aatagttgta gtttttacag ttctattttt aacaaataga
    16561 acttgatcaa ttaattcttt caaatagtaa aaacgatgtt cagctataac aatagtaaat
    16621 cctttatttt ttaaatcaca aagtaagtca gcaagtttct tagtcgcttt gaagtccaaa
    16681 tttgaagacg gttcatccaa tataattaat ttagagttta gcgataaact acatccaata
    16741 gaaattttct gccgttcacc acttgatagc gaaaaaatgt ttttatctaa tatatttgaa
    16801 agatctaata attcagctaa ttcattgatt cttttatcca tatgctcttt tgtaaatcca
    16861 aagttttcca tcgaaaatac taactcactc gttgtatttg ttgtaaagaa ttgtcctcta
    16921 ggatcttgac ttacgtagcc aatatttttt gatatacttt cttgtgttaa ggattgtagc
    16981 gagttatttt caaataaaat ttccccaacc aattttccgt cataaagtct tggaataagt
    17041 ccattcatta actttaatag tgtggatttc ccactcccag aagaaccagt aatcaatgta
    17101 agactgcctt tttttattga aaaattaagg tcaacaagcg cttgttcctt aacatcctta
    17161 tatgaaaaag aaacattgtt aaattcaaac aattctttca cctcaaatca aaatacttaa
    17221 gaaaaaaata gtaacggaaa acaggatgat atagtctaat aagctcatgt ttgttttata
    17281 gtaagaactt cttttcacag gagattctat cccctttgtt attgcattag cggtcatttc
    17341 ttcagcaatc atagttgcac gtataagaac gggaactaac agaaattcca aatattgcag
    17401 aggctgaaaa aaagacaagc gaatgtttct catttttaaa gattctttaa tcattcttat
    17461 ttcagaacga taagtcggaa agaatctgat cactactgta atacttaaca ttattttttt
    17521 cggtaaatgt attttttcta aagaggataa taattcattg ctagtataga cactaaccaa
    17581 caaccatgag atcattacga ccggcaacat tcgaaataca atatataagg atgtcgcaaa
    17641 aaatatagta aaaaactcta tcttgatagc atcaataatt ttcattacta aaaacagaac
    17701 tgaataaatc aagatgaaat aaatacatgt ctttatttta cgaaacatca atactaaaac
    17761 tgtagcaata atgaaacacc ccacttgaaa tatatttccc tttcctaaaa aaataatgac
    17821 cggaaaaaaa atatttaaag taatcaaggt taacggattg atttttgaca ctctttcacc
    17881 tccaaaaaat aagatcgcac tgttatctta agctgataaa aatgcaccgt tattcagata
    17941 acagtgcgat cttatcaaaa agcataactg agaacaattc tcaatgcaag ctttttttaa
    18001 ttaactcagt aattcaatcc acccaggaac agaaaattga tacaatcttc tggttaatag
    18061 catagtcttt tcttttgaat agctatgctt caatatatca tagaatgtag cgtagaatga
    18121 gtaaatgtag aagtggattt cttcttcatt aaagactaaa ttttgattga tcattccgat
    18181 agtctttaac ttatctagac aacagactgt ctttttcgtc acaaatgatg taattttttc
    18241 aacaatatct tgatatttgg ttccagctga ttggaataaa atcaaatcga atatacgttt
    18301 attctcaaaa atataattaa taaatggatc taattcattg aagttctctc tccaactttt
    18361 ctctatatct atattttcta agtttaagag gtatttggta aactcttcat tatacatatt
    18421 aataagcgca ttaatatgcg gctgaataat ttcttcaaat aacgcttcct tagaagtaaa
    18481 atgtttataa aaagcacccg tagttacgcc tgcggtcagg cacagttgcc ttatcttagt
    18541 ttgctgaaat ccttcttttt caaaatggct tagtccactt tctattaact tttggtgagt
    18601 tataatataa tctctggttt ctatgggtac ttcctcctaa taagatgaat taactttatt
    18661 atcttacaca ggacttgaag tttcaatcgt taacgtttat tactgaactc attccttaga
    18721 gctaaactaa tgagaaagag cagaataact gatacaaaaa gcaatcctac aagaattaat
    18781 agactcatct aaatcattca ccttttctaa aataatccga gaaagattta aaaaaattat
    18841 atccagctat taggataagt cctatcataa tcactccaat agccattttc ttatcctccc
    18901 taaaaataaa gtcttttact taaaaccttt tttgtaatcg tcaataaatt ttttcccttt
    18961 atcagttaat tgaaatccta tatctaaata gtcctcatat ctaagtttag ccagcatctc
    19021 acttacagat gctggactaa cacttagaac ttttgctaaa tcattatttt ttaatttctt
    19081 ttttcttttt gataattcct gaattgctag taaataaatt agtatagtag tcgttttaat
    19141 ttgtaccata cctaccctcc aaattatttc gaaaggcctt cagcaatttt atccaggttc
    19201 catttcatca tcgcatagta actatcacca tcttgtcctt tttctgcaat tgaatcagta
    19261 aaaatcgttg aatagatagg aatattggta tcttttgata ctgttttcat tggtctatca
    19321 tccacactgc tttctacgaa taaggaggga acttttgttg tgcgtaattt ttcaactaag
    19381 tgttttattt gatctggtgt tccttcttct tcagtgttga tttcccaaat gtaagcagaa
    19441 ggcacattat acgctttcga gaaatattta aagcatcctt cacttgtaac aatcattttt
    19501 ttatcattcg gaattgaagc aaatttagat ttagcttctt tgtctagaga atccaatttt
    19561 tcaatatact tatctagatt ttctttatag aattttttat tatcaggatc tttttccgct
    19621 aactgttttt caatattttt agcgtaaata ataccgtttt ctaaatttaa ccaagcatga
    19681 ggatcttcct tccctttctc actctgaccc tctaagtaaa taacatctat gccatcactt
    19741 gctgcaaaat agtctttgtt ttcctctttg ttcgcatttt taactaattt tgtaaaccaa
    19801 gcatttcctc cagtttccaa gttaacaccg ttataaaaaa tcaaatctgc ttttgaagtt
    19861 ttttgaacat cttcaggcaa aggttcatat tcgtggggat ctttcccaat aggtacgata
    19921 ctgtgtaaat caattttatc ttttgctata ttttcggtaa tatctgctaa aatcgaatta
    19981 gtaactacta cttttaattt ttcgttcgaa gccactttat ctttgtcttt actatttatt
    20041 gaacatgccc cgaatataaa aagagcaatc aaaatcccca gacttcccag tactgctttc
    20101 cattttctca tcataacgat ctctccttct ttcttaaaaa tttttgtttt ggtgagatta
    20161 gaaaactaat tacaaataag aatgccgctg ttaaaacaat acttgatcct gctgctatgt
    20221 taaaactata accaataaac aatccaagca atgatgaact tgctccgact gttgcagaaa
    20281 taaatatcat agttttcaag ctatttgcat ataaatatgc tgtagctgct ggtgtaatta
    20341 acattgcaac aattaatata gttcccacac tttgcatagc tgttactgct actaaagtta
    20401 atagaaccat caatagataa tgataaaaac tgacattcat ccctattgct tttgccatta
    20461 atggatcaaa agaagtgatt aataattctt taaaaaatag aactactata accaacacta
    20521 aaaccgaaac gccaattgta atccacttat caatatcttg tacagctaaa atatttccaa
    20581 ataaaatatg aaataaatcc gttgagctat ttgcaactcc tataaggata actcctagtg
    20641 ctaaaaaaga actgaaagta ataccaattg ctgtatctcc ttttattaca ctattgcttt
    20701 ttataaaagt aataatgata gaagctaata gaccaaatac aatcgcacca ataaaaaaat
    20761 taatacctaa aatataagaa attgctactc ctggcaaaac agcatgagaa atagcatcac
    20821 ccataagaga cattcctctt aaaataataa aacaacctac tgttccagaa acaatcccta
    20881 taactacaga tgtaattaga gcattctgta aaaagtgaaa gtcatacaga ccattaataa
    20941 aattttgaag catattattc cccctctcca ataaatatag tatctccata ggcttttttt
    21001 aaattttctt cattaaatac agattctaca cttccgtgag caattagatt tcgattcaaa
    21061 agaataatat tatcaaaata cttctttacc ttgcttaaat catgatgcac aattagcact
    21121 gtttttccct gatgtttcaa tttttttaac gtatccataa ttattcgttc actaactaag
    21181 tctatcccta caaatggttc atctaaaaat atgaaatccg cattttgagc aagacatctt
    21241 gctaaaagta cacgttgaaa ttgtcctcca gataattctc ctatttgatg attagaatat
    21301 tttcccatat cgaccttttc caaagccttt gaaactcttt gccattctgc atttttaatt
    21361 ctttgaaaaa ctttcattcc ggcatatgtt cccatagata cacattcttt tacttttatt
    21421 ggaaaagtaa aatcaatatc agctttttgc tctacatatg caatcttcac cagtttttta
    21481 ttgatatctt cattattaat ggagacctct ccctgatgag ggataatatt taacattgct
    21541 ttaagcaatg tggattttcc agcaccattt ggaccaataa tacctgttaa agcaccttct
    21601 tgtattttta aagaaatctc gttcaatgct aaaaaatcgt tataacaaac attaagcttt
    21661 ttcacttcta acataacttt ctccttctaa atgataatga ttatcacgat catatggtat
    21721 acaaaaatta tattttagtc aaccctaaat tttatagttt agattttttt ctgttgctga
    21781 agattagaat gacaaaacta tttgaaacta cgcgtatagt aagtagcttt caaaatatat
    21841 aaaaaaggag cgttctcaaa tgagaacatg ctcctaaaac tactttattg gcgtatggaa
    21901 aaattatact tgaagtggat agcgggttga ataagttgat aagcatcaaa acatttataa
    21961 aaattattca agactatgtc tgttccatat acaaatatga aaatcagtaa actccattta
    22021 acttttttcg tagtgtgtct ttttatacat gtttatcgtt attaatagtg aataggaaaa
    22081 aaactatttt tgatcattga caattaaata caatttttga ataacgatcc ctgctattcg
    22141 agctagaaac tcaagtacgc catgacctga ttagcgacaa atacttagag atttcttttt
    22201 gttctgggaa aacaaaatgc catgacaata gcagcaaata atgatacttc cgtaaggctc
    22261 gccgtaaact ggggagaggt gaaattccta tgtgcttacc caaagcaatt caaaaatttc
    22321 ataactacta ttttaggaga tgattttata gaatccagag tctacctgaa taaaaaacaa
    22381 acctgtgcat ttctaggaat cgataataat actctagata agtggattcg tatatataat
    22441 ttcccagtaa taagagttgg tggtgtagtt cgctttgacg tagaagatat taaagacttt
    22501 atgaatacat ttaaaataac ttgaaatttt cttctcgccg catatactaa aattagtgaa
    22561 atatactgcc ttactttttt taaggagtga ggattttgcc tacaaagctt tcaaatggga
    22621 aatataaaac taatctgaga tatccaaaaa ggtttaaaga aataacagga attgcatcag
    22681 aaaagtatca aaaaacattt ccaaataggc aattggcgat taaagccgaa aatgatatga
    22741 agaagaaaat agaaaaagtt ttgagagagg agaatgcaaa ctcattagaa ttaaaaggaa
    22801 aaataacgtt taaaaaattt tacgaatcta aatggcttcc tagatatgaa ttaggtcaaa
    22861 caattagatc aaacaggcct cctagtgata taaccattag taatacaaaa gatatatttc
    22921 ggttgcatat tttacctatg tttggtgaat acgcaatgaa ttatctaaat atcaatactg
    22981 agataatatc agatgaatta accaaaaaat cgaaagaata tgcaaatata aaaatcataa
    23041 aaggatatgt tcgttccatg ttcgatattg cagaaatact aaattatatc gaatttaatc
    23101 gaactactaa aattattcaa agtatcactg cacctaaaaa aaatgcttta gaagaaaaaa
    23161 gaatacaaga aggtaagcaa gcactatcat caaaagaact tactaattgg atagaagcag
    23221 taaatgatga ttttaataat catttactta cttttcatga ttacacactt tttatgatta
    23281 ccctgtatct tggtgataga aaaagtgaga cttacgcttt gcaatggaaa tatattgatt
    23341 ttgaaaaaca aacggtccgt cttaaacata ctttagataa atatcaaaga aaaaagttta
    23401 caaaaggtcg aaaagatacc gtcattcaag taccagaagt tgtaatgaca ttgctaagtg
    23461 aatggaaatc ggtacaagct gatcaattgt tgaaattaaa aattaagcaa acgttagatc
    23521 agtatctctt tacttatact aaaccatctg gagaagtaaa ttgtcctgtt catgccgatt
    23581 acctaaacta tcgaatcaat tcaattaaaa gaaggcatcc tgacttagtt catttatctc
    23641 cacacaaact ccgtcatact tatgcaacta tagctagaca aggtggagca gatatgaatc
    23701 aaatttcaaa tgctttaaca cactcagata tttctacaac aaagatatat gtgaacacac
    23761 cagatatagt tgataaggct gtttttgaag cttttcagag aggattgaat aagtgtgatt
    23821 gatatgtgtt cttaaatgtg ttcttaaaaa caaaagaagc actttccaaa taattgaaaa
    23881 gtgccagaaa cgttgatata taaaggatat taacgttttg agaattgtga agctttacgt
    23941 gctttcttaa gacctggttt tttacgttcg accatacgtg cgtcacgagt aagtaagcca
    24001 gcacgtttaa gagctaaacg gaagtcagga tcaacttcta gcaatgcacg agcgatacca
    24061 tgacggatag ctcctgattg tccagcgtat cctccgccgt ttacgttaac aaatacgtca
    24121 tatgcgcctt ttgtttcagt aacagcgaaa ggttgattga ttacttcacg caagtcagca
    24181 tgtggaatgt attcttcaac atctttttta tttacagtaa ttttgccagt tcctggtact
    24241 agacgtacgc gagcaactgc gtttttacga cggcctgtgc cgatatattg aacttgtgcc
    24301 aatgaaattc cctcctatta gattaagttt gtgatgtcta aaacttctgg ttgttgtgct
    24361 gcatgtggat gttctgctcc accgtatacg ttcaattttg taaattgttt gcggcctaaa
    24421 gtgtttttag gaagcatacc ttttacagaa gtttcgatca aacgacgaga atttttagca
    24481 cgcaattcgc cagcagtgat tgatttcaat cctcctgggt agttgctgtg acggtaataa
    24541 attttgtcag tcgctttttt acctgttaat ttcactttgt ctgcattgat gacgatgacg
    24601 aaatcgccag tatccaaatg aggtgtgaaa gttggtttat ttttaccacg tagtacagat
    24661 gcaacaactg ttgaaagacg tcccaatgga acatcagttg cgtctactac ataccattta
    24721 cgttctactt cgcctggttt ggccatatat gttgtacgca cgttattttc ctccaattta
    24781 agttctgatg tttgacatac acttcgagtt tccggggctc tttgtggggc aaacaatacc
    24841 atttaacata ttacccgcta atctagtcaa tgtcaacgtt tttctgcttg attgaaaaat
    24901 ttatatgaaa ctttacttct tttctttctt ttatataaaa gggtcgtttc ctgtcaaaat
    24961 gaaaaacaaa aagaggctgt gacaaaagtc ttgtcacaac cccttatttt gaataaacgg
    25021 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc ataaaacata agaagctgtt
    25081 ttttaaaatt gtttcttgtg actcggagct gaacggcagg cttacaacct cttggtaaac
    25141 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
    25201 tttgacgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctttttcgg
    25261 tcaagttcaa caacagtttt tacgcgtcga tctctggcgc tttgcccaaa tgagcttgga
    25321 tcatccagat acgtttttct gtggctgttt tgaattcgat aaagatatct tgtgttggat
    25381 cgtcgccttc ttctccagaa acttcgattc ctttttggta aagatctgct agataacgat
    25441 agcctgcaac aagtgtttcc aaacgttctg gaatagtacg atcccagcgg ccaatttcat
    25501 cagggatttt tgtgttatct gcaaattcac gcaatgtaga ataaggtgaa ccgtctaatg
    25561 tgatcaagcg ttcggatact tcatctaact gagcgttcaa atcatccatg aactcatcca
    25621 tcattgggtg caatgtaagg aaacctggtc ctctcatgta ccaatgtgtt tggtgaacaa
    25681 caacagacat ttggcttaga tctgcaacta attgatttaa tacttctttg gtttgttgga
    25741 atttcatctt tcttgcctcc taacattttc ttacacttaa aataatagta gactttcaat
    25801 gaaaagtaca acaaaatcat ttacctgaga acgattttca gttcgctttt ttatccgtaa
    25861 agccaatcat ttttcagaat cagctaattc ttctcgcagt tgagcaaatt gctgataagt
    25921 ctgtgctaaa tgcttttgtg tcgaaacaaa taatggatag attttctgat attttttatg
    25981 atcagtttct tgtactccaa cttgctcttt caattctatc aattcctgtg cttcactgat
    26041 actttggatc tttccgatgc ttctccagcc taaaatcaca gctcctaatg cggatgcttc
    26101 tgtgacagta gggaaaacaa gcgggtaacc cagtacatca gccatcatct gacgaaaaat
    26161 cggagattga gcaaacccgc ctgtagcacg gatctctgtg agctgatttt cctggcaaac
    26221 tccttttaaa atccgataaa ggttgagaca gatcccttct agaatcgcct tcatcatttc
    26281 tttttctgta tgttgccgct gtaaaccgat aaaactgcca aacgcatctg cgtcccaaag
    26341 cggtgctcgc tcaccaagta aatagggatg aaaaatcaga ccattggcac ccgctggcac
    26401 aaaagcgata gccgaaagca gtgcatcata tggattttct cctttttctt tagcagattg
    26461 aacgacttcc tgtaaaaatg tatgccctgc ccaatcaaag atgccagcac cattgctagt
    26521 cgctcctccc actacccaat gtttctgatc cagaacataa caaaacgtct ccatttctgg
    26581 atggaactga ggctgagtag taataaaacg aagtgctccg ctcgttccta cggtcaaagc
    26641 ggcgatcgta tcaccaaaag cacctaaccc tagattagaa agcggcccgt ctgcccctcc
    26701 caaaacaaat gatatatcat ttggcaaacc tagttctgct gctgtttttg atggtagacc
    26761 tgttacttga taggtggaag gaaagacttg cggcaattgc tctattcgaa tgttcaagta
    26821 gtcaagcact tcttctgccc attggaattc atggatatca aaatatccag ttcctgaagc
    26881 tgttccgtag tcgctgatca gttcgccaaa caatctataa aagatatagt ctttgatacc
    26941 gacaaaataa gcagtttttt tgaataattc aggttggctg ttttgcagcc acttgatttt
    27001 gttcaatggt gtcattggat gaatcggcgt cccagtctgc cgataaaatt ctttgccaag
    27061 cacggtatat ttcagttttt ctgcttcgtc cgctgctcgg ttatctgccc aagtgatcat
    27121 tcgagataac ggtgttttct cttcatccaa caatatcagg ccatgcatcg cagaggagaa
    27181 ggagacaaat tgaatgattg tgtctgaagg caactgttta attacttctt ttataacggt
    27241 gcagacacct tgaaagatca attcctgatc ttgttctgcc atcccttgtg tttcttggat
    27301 caaagggtag cgaaaataag cggatgctgc ttcttgggct tcatctgtga aagcaactgc
    27361 tttggtttgt gtggttccga tatctaatcc gattgaaacg ttcatttttt cacctcaaat
    27421 tttatgtatg tacaaaaatt tagtttaagc ttgcgtatct tggttttgaa aagggggaga
    27481 tggcgtgtta ctttatttta tcattggttg ctgtatcgga tcatttttat gtttaaccgc
    27541 ccagcggatt ccgttaggtc actctattat ttatcctaga tctcattgtg tcaaatgctg
    27601 tcgttcgctt tcttggtatg aattgattcc gattctctcg atcatcgtcc aacgatttcg
    27661 ctgccgctat tgcaggtgtc ggttgccctt ctactatttg ttggcagaag ccttatgtgg
    27721 tggtcttttt gcctggttct ttactttttc ttccgcaaga aatttttcga cgttcatatg
    27781 gatgctttcg gctttgctat tttcgttgat ggatttattt tacttcatgg tccattctca
    27841 cacgctattc tgttcttggg caattttatg gattttttgg ttgcaggccg gcgtcttcca
    27901 atggcaaagt gtgctgttaa cgtttatcgt aggcggtgtg tgccttcgct atggtcaatc
    27961 ctatctcgga gctggcgata ctctgttgat gctttcttgg agtggtggct tgtctctaga
    28021 agagttgctt cagatcttgt ttcttgctag tctgtttgga atatccgttt tttctgttta
    28081 ctttatctgt cataaaaaaa agttggaaaa gctgcctttc attccttttt tgagcagtgc
    28141 gttgctgatt gtccttcatc ttaggtattg aaaaaaggtt gtgacaaacg acttgtcaca
    28201 acctctgata tatagtgttc tcttaccaaa tccttgtgac ttggagctag gcgacaggtt
    28261 cacaacctct ccactaaatg gtgttcaatc agctgaccct cagtattagc tcaaaatgct
    28321 ggttttacaa cctctgatat acggtgttcc ataagtagct ttttcggctt cttatttctt
    28381 gacgccccaa tgtcgacgaa ggaaacctgc acgtccagaa ccttctcgat aaagattgta
    28441 atggagcttg tttttcttgt aataatcctg atggtaatct tctgcaggat aaaattcgct
    28501 tcttggttcg atttttgtca cgattggctt atcgaatcgc ccgctgtctt gcaatgcttg
    28561 ttttgaacgc tctgcaatat ttcgttcttc ttcatcatta tagtagatca ctggacgata
    28621 agaatcaccg cgatcagcga actgtcctaa tgcgtcagtc ggatcggttt gctgccaata
    28681 gatttccacc aactgatcat aagaaataac ttctggatca tattcgatct ctactgcttc
    28741 tgtatgccct gttgtgcctg ttgttacttg ttcataagtt ggatttggta catgcccgcc
    28801 agtataaccg gatgtcacag aaatgatccc tggctgctct tcaaatggtt tgaccataca
    28861 ccaaaagcat ccgcctgcga aaactgcttt ttttgtcata taaaatcccc cttttttctc
    28921 atcatacctt gatttccaaa aggaggataa cgttttgctt gtgacagaaa aaacggaaac
    28981 tattatatac gtgatacggc ttctgataaa tgccaaaacg aacgtacttg atcaaaaacg
    29041 gttaaatcag gaatcacata ttcagcagaa ggaagattta tgttttcacc tattcccaat
    29101 acatgcatgt taccagccaa agctgcttga caaccagctt ctgaatcttc aatgaccaag
    29161 catgcatttg gcggtacatt caattgctgt gctcctttga gaaaaacttc tggatcaggt
    29221 tttgcttttg atacttgcgt gccgtcaata aggacatcaa aataacttgt caaattcgtt
    29281 ttttctaaaa tgaggcgtgc atttttgctt gctgatccca atccgatctt gatgtttgtt
    29341 tttttcaagt actcaagaac agctattgca ccaggcaaaa cggatgtttc atccattttt
    29401 tgaatcgctt gaagatatag attgtttttt tgctcagcaa aggctgattt ctctttttct
    29461 gagaatttct gttccatttt tccaaatgac agcaatcgct ctagtgactc ggttctggat
    29521 attcctttca gttgctcgtt ttctttctca gtgaatggaa tggacagctg gttagctaaa
    29581 accagccacg cttcgtaatg ataacgagct gtgtctacaa gaacgccatc taaatcgaat
    29641 agtgctgctt tgattttcat cgattacctc cttgaaaatg aacgacttga agtaaagatg
    29701 cgatgttttt agctgtgcga tgaagattat cagtggcttg ctgtaaagct tcttgtaaag
    29761 gcacagctgc aggaaggatc ggaaaaacgg ccgaaatgcc aacatctgct gctttttcgc
    29821 ttcctttacc aacacttccg caaatagcta tcacaggaac gccctctggt actcttctag
    29881 caatcccaac tggcgctttt ccttttagac tttgtccatc tagacgtcct tcgcctacga
    29941 ttacgaggtc tgcttgctgt acccgtttat cgaactccag taaatccaat acgtagtcga
    30001 tgccagagac tattttccct gaagcaaatg ctaacagtcc tgcacccatt ccaccaccag
    30061 cactactgcc cgatgcctcg attacttgag gggcaaatct catataaaaa tcatgcatgg
    30121 cttggtcagc cactgctaat tcattttctt tcaagccttt ttgtggtccg aaaacaaaag
    30181 aagcgccttc ttttccgcat aaaagattag taacatcagt aatgacagtg attttggctt
    30241 taatcagctc aataggcacg ttcgttttat caactgtagc aattttacct agattttctc
    30301 ccagtgcttc tactttttga ttatcttgat cgtaaaaagc ataacctaga ccttctgcca
    30361 tgccgatgcc gccatcgttg gtactacttc cgccaacgcc gatgatgatg tgtcgaacac
    30421 cttgttgcaa caattgtcgt atcagttctc ctactcctcg cgtggataac ctaagtggct
    30481 gttgttggtt tagtgggaca tctttcaatc cgacgatttc tgccatttca aaaatcgcag
    30541 tttgaccgtc tgaagcgtac tggatttctt tgtttttgcc aaatgcatta gtaacgactg
    30601 ctgtttgccg cttcaacctt aaactgtctg ctaatgatga aaccgttcct tctcctccat
    30661 cgcctatagg tagcagatca tattcagcct gaacaaaaac ttcttgaaaa cctgattgaa
    30721 tggcttgtgc tgcctctaag gctgtcagac tttctttgaa agaatctggc gcaatcagaa
    30781 tacgcattat tttcccctcc gttccaagat cagttctttt tccagctggt attggtctcc
    30841 attgacaaaa ataggtaatg tttctttcgt ctcttttttt attactattt gtttttttgt
    30901 caaaacaaat agcaacctgt gatttttcca aataaaagga aaagaaattt tttcccatgc
    30961 tgaaggaagt cttggagaaa tagaaagagt tccattcgtt actgtaatac ctgaaaaacc
    31021 aaatataact gctagccata gtgctcctaa tgaagcagca tgaattccgt catctgatga
    31081 atgcggatct ggtcctaaat caatcaggca cgcttcttgg aataaatgat acgcccattc
    31141 catttcttgg atacggcaag cctctatggc gtgaatcgct ttactcaacg atgagtcgtg
    31201 gattgttttg ttttcatagt aatgcaaatt cttagaaacg attggttgag aaaaatactc
    31261 aggtaataag tataacagca tgacaagatc tgcttgcttg ctgacttgga gttcatttac
    31321 ttcgctacgg gcataatcaa gtaaaataga ctggctgcca ggacgttctt tgtaagtggt
    31381 caaatcaata actggcttac tgagatacgt gtcatcttgc ggaatcagct gttctttgtt
    31441 aggcactggc aaatataact tatctaaaaa attttgacat tctttcaaaa aaagcggatc
    31501 gccagcttga tatttaacag ctaaggccac attataatgc gccagatagt tcgtatatgc
    31561 attattgtcg atatattctg tgtattcatc tggaccaatg actctgtgta tctctaaatg
    31621 atcatcgact tttacagctc tgctcagcca aaaagctgct gtttcatgaa tgagcgtttt
    31681 tccctttgtt tctaaaaacg attgatcctc tgttgcttgg acatactctt gaacggcata
    31741 agcaaggtct gcactgatat gatgttctgc ggcagctgaa gcaacttctt gtcttgttcc
    31801 ggttttgata ttcaatgcag caaatttggg tgtctcttcc tgaccagtgt gtgcgctttc
    31861 ccatgggaaa agtgcacctt gatagtgata atctcttgct ttttgccgtg cgccttctaa
    31921 tcgatcataa cggtacagca gcaaattctt tgctacttca ggaaactgat ataaaaagaa
    31981 aggcaggata aaaatttccg tatcccagaa aacatgccct ttataacctt cgccagttag
    32041 tcccttcgcc cctacactaa cacttggatc attgcgggga gtcataatct gtaaatggta
    32101 agcagcgaag tccatcgcta actgatcgaa agaagaatcg ccttggatcg ttatccgatg
    32161 ctgatgccaa aaatctgtcc aataagacat cgactgctct aaaatttctg tataactaac
    32221 gcttttctgg gcttgggcaa tacttttctt aaacgggtct gtttgctgaa gagaggtaga
    32281 gagcacgatg atcttctcca gcgtaaagga cgtatttttt ctgatctttt ctgaaaagga
    32341 agcggtgatc cggcgatttt ttgcttggaa tacacctgct ttattgaatt tagctgcaat
    32401 cccaatatca attttacttt ctgttgtctg gtaggatgca ctgatcaaat cgtcaccaaa
    32461 tactcgcagt tctttttcaa ccaactgctg tgtaccaaaa ttggtttgct gtccatcgat
    32521 acccgttttc acatctactt ggcagtcttc ggtcaacggc ttgatgacca ctttcagggc
    32581 tgcctgatgg agattatttt gatcagcaac acgacaagta atgacttcca ctttttttcc
    32641 ttgagcagtt tcaatgagcg catagcgttt aagctctccc atttctaaat ctagtgcccg
    32701 ctcgtatgca agaactttca tcgtctcaag agaaaaatgt tcaccgtcga tttttatttc
    32761 ccatctgatg acatctggga gattcactaa ctcggctgat tctgctccta aaggtcggtt
    32821 atatacacca gctgcaaaca ttccccttac ttgattccga tagtcttcct cctggcttcc
    32881 tctgatacca aaaaatccat ttccaatggt catctgactc gcatacttat tgatatactc
    32941 taaatcaaac tgtttttctt ctactttcat aaggtaataa cttctcctgt ttctgccgaa
    33001 cgatataaat tttccaccag attctggatg acatatcctt gccagccatc tgcaatcatc
    33061 tgagtctggt caccgagcgc ccgattcaca aaatgagcca tacttagttc atgtgggtca
    33121 ttaactggcg tggtcttttg taaggaaacc agctttccat ctacatcttc gaatagttcg
    33181 cctggataca gattagcacc tgcctgatcg ccacaaaaag ccacattcag tttgtcttct
    33241 ctgatattca aagcaaacga agtattcagt tggatgatct tgccgttttc taattccagt
    33301 gtgccaaaaa ttgcatcttc tactgtgaat ttgcttggat cccatttccc gaatttccct
    33361 gaattttttt tcgtaccaat tttttggaag ctgtacgccc gaacgctttt tactttagga
    33421 aatccagtaa tatacatcgc tgtatcaagc atgtgaatgc cataatcgat caaaggcccg
    33481 cctccttgaa gttctttgtc gataaacgtt ccccagcctg gaacaccaga ccgacgaagc
    33541 gcattcgctt ctacgtaata aatatccccg agaacatcaa gatgattctt gatcattgtt
    33601 gcttcgtcag aaaaacgatg ctggaatcca taagccaaaa tctgcccgtt tttatcggct
    33661 gtctctgcca tctttcttgc ttgatcagct gtcatagctg gtggtttctc gcacatcact
    33721 gaagcattag cttcgagggc agccaataca tgttcgaagt gaaagcgatt gggtgtacag
    33781 acactcacga tatcgggaga aatctcattc agcatttgtg taactgatgt atacgcatga
    33841 gaaaatcctg tacgtcggca aaagtcttct gctcgctggc tgactggatc aaccacagcg
    33901 gccaattcta agacatctga tcttttttga taataagcag catgtacttt ttctgctact
    33961 tgtccagcgc ctatgattgc tactttcttc atttatttct ctctcctttg cttatttact
    34021 tattgttatt ctttctctat tctttctcta aatcatttaa atgacgcgct gtttccagac
    34081 agtttttcaa gtattgcaat gattcctgat aaattttcac ttcattttct cctcgcaaac
    34141 gacattcata taccatataa ccttcatact cattttgaag tagcgtttga aaatggcgag
    34201 taaaatcaat tgcgccagat cctggttggt agcgatgatt atctgctaaa tggatatgac
    34261 ccacatactc tttttgcgta gtcaatgctt ttgaaatatc atcttcttca atattcatat
    34321 gataaaaatc agcgatgatc tgcacatgtt tcaattgatt agagtcaatg taatgacgcg
    34381 catccgctaa caaattgatc atatggtctt ggtatcggtt caaaggctcc aaataaataa
    34441 ccgtacctgt ttcttcggct actttatcta aataacgaag tgattcgctg actgctttat
    34501 agtctccttc tttgcttcgg ggtgaaacca tcggcggtaa tcgataagtg aacattcccc
    34561 aagctgctgg gataacgatt cctttgcctc caacctcgct tagtgcacgc aaaatctcag
    34621 caatttgacg tacaccatta agacgccgtt cttcgataaa gtcaccgatc cacccatcgt
    34681 agccgttaca agctgtcact actgggatcc ctgtttcttt gattgcctgt ttgactgtgt
    34741 cgatattttc tattaacaac ttaccgtcga tttcgtattc ttcaaatcca cagtctttga
    34801 taaatctaaa tttttctaaa atgtctcttg ggaaaaatgc ttgatcttgc gtaccgaatt
    34861 tcattgttct cctcctcgga cttgaacgcc cattttgata ctttcttctg ggtgctggtc
    34921 tacataacgc atgtaagctt ctgcgctttc tgcaaatggc acaactggat caatgatttc
    34981 ttcacaatcc aagtaaccgt tcatcaataa ttcccaacat gtgtcctcga ttcgtttacg
    35041 gttccatctt gggtaatctg ggtttggttc actagatgct cgagcaaaga cgatttttgc
    35101 attattgaaa tgtgcttcgc gaccaagatt aaacccagct ggaaaaggtt tagcaaaggc
    35161 aacataagaa atgatcccgc catagccgat ccctattaaa gaagcttgta atgcggcagc
    35221 actgccactc gtttcaataa ctgaatctac tccacgttta tctgtcaatt cctttagacg
    35281 aaaaccgaca tctgctttca taggatctag cgtgtaatcg gcaccgtgtt tttctgctaa
    35341 ttctctacgc tgcgctatgg gatcgatccc aacgatggta gtagcacctg cttttttagc
    35401 taattgaatc gcaatttgcc cgatagcgcc taaaccgaag attgctacag catcacctgc
    35461 tcgaacatgt gcttcccgga taccacctaa agcgaattga gcaggatcgt aacatactgc
    35521 atttttccat tgatccaacc gatcaagttt acgtagttta taattatcaa caccgtttgt
    35581 aatcagcgtt tcacgaatgg gtccataagt gcagactaac tctccttttt gatactctgt
    35641 caccaaactg cccgtttctt cgatcgtacc gacaatcata tttcctaatt gaaattcgcc
    35701 aaataccaca ccgcgttggc ttccttcttc tcttggcaag aataggcgcc attcttctga
    35761 aaattcttca tcgataaatg gacttaatcc tctaaagtcg acaatttctg ttcctggttt
    35821 tggagtagcg aaaattactc ggatacgtac ttcgttcttt tcgatctcac ggtcttcgta
    35881 atctttgaat ccggctattc ttggttctaa tgcaactaat tttttcatct tttctttcct
    35941 ctttcttttc tttttatcct tttacgccgc cttcgactaa atcaccttta ataaatcgtt
    36001 cagataacgc atacataatg atgactggta aagcggtcac caatgaagcg gccatcattc
    36061 gtccccaaat gtagtctggt gtactgaata atgtgttcaa accgattggt aaagtaaatt
    36121 tttcggaggt cgacaggaaa atcgaagcaa ataaataatc attccacgaa accataaagc
    36181 aataaacgaa gacagaaatg attccagaaa tagccaatgg tataatgatc cgaataatga
    36241 tctggatccg gctaagacca tcgatcattg ctgcttcttc aatatcagtc ggaatcgtgt
    36301 caaaataact ttttagcatg aatatcgcag taggaagtgt ttgaacgacc atcgtgataa
    36361 tcagtgctgt attggtatca tacaaaccta gaccggaaat gattttaaat aatggaacaa
    36421 cgatcaaaat accggaaaac atataaacgg tgtaaaagct agcattgatt ttcgagcgag
    36481 ctttgaagcg tagttttgac aaagcgtatg ccccgctgat cccaatcact actgcagtta
    36541 cggaggctat caaggagatc actaaactat ttttgaaata catgatgaat ggaaaaatct
    36601 cagcattgaa aatatcctga taatgttcaa aagtccattc tttcggaaaa atagtttgtg
    36661 tcgttgcaat tgcttcttgt gaatccttga atgatgtcat cagcatgatc aaaaatggaa
    36721 acaaattgac agctaatagc aacaaaatcg tcgcgtaaaa taagattttc ctgctctttt
    36781 tatttttcat ttcaagtcca ccttctttcg tgagagcaag acgaccgctg caataatgat
    36841 gaacaataga atcgatatag ctgctgcttt tccaagatca ttgaacgcaa aggctgtctg
    36901 atacaaataa acgcctaaga tattgacttt attcgtcagt agatagacat ctgtaaacat
    36961 atagaacatc cagatagatc gtaaaacaat cacagttgct agtactggtt tgattgccgg
    37021 cagtgtcacg atcttgaact tctgccaagc atttgcaccg tccatttcag ctgcttcata
    37081 aagcgttttg tcgactgttt gcaaaatagc caaaaatgaa ataaacgcat acggatagta
    37141 tctccagata gcaaacaaaa cgaccataaa gaagctagca actggctggt cgaaccacag
    37201 cggtgcttcg ttgaaaagct gcaacctatc tactaacaga tcattgacga ttccataact
    37261 attattgaac atatatttcc aagcaaaaat caaagaaata gatggcgtaa cataacttaa
    37321 taaaatcagt gaacgcaccg ttttcctaaa tctgaacttg cggttaagca aaatagctac
    37381 ggctaatcct aaagctgtac tgcctgctac aactaagaat gtgtaagcaa ctgttaaacc
    37441 taaagaacga taaaattcac tatcacttaa aatttcaaga taattgctta atccgataaa
    37501 aacggcgtct gagttaggat tcagcggcat atcgaaaaaa ctgatctgaa tattggaaag
    37561 cattggaatc agcactaaaa cagctaataa aagcatgctt ggtgctaaaa gtcccatggc
    37621 taattttaag tcagaatgtt tccttggacg attcattgaa acctccccct gataatctgc
    37681 caatagctat ctactttttt cgaacaccat tggcagaact tcttgttatt tcgtattttt
    37741 ttgtgcgtca gctaaagctt gatcgacatc ttcgtttccg acagttactt ggttcactgc
    37801 ttttccgatc acacctgagc ttgtgatatc tcccattttc gtgaaattct tttccccgac
    37861 tagtccaaag acttgtactt catcaaaagc actagcaatc tcagatggca gctcgccaaa
    37921 agacttgatg acttcatttt ctttgtaacc atccagttcg actacttgtt tattcactgg
    37981 ttgagcacca ccaggagaca tcaagaccca cttttccatg tttttcggtt gagataagaa
    38041 agctgtgaat gcttcagcag cctctttttg tttctcgtct agaccagaag aaatcgtcaa
    38101 tccagatact gtgccataga cagcttgatc tttttgtgtt gggatagcaa aaccgatatt
    38161 ttctgagttc cccgcttcat aaacagaagg caaaatgtaa gttgaataga ttgccattgg
    38221 tgctgttcca ttcatgaaag catctttgat ttctgtcaca tcattagagc ctggcatcgt
    38281 gtattgggat aagttttgat aaaacgacaa tgcttctctc atcttttccg tatcgatcgt
    38341 tacctcgcct tcatcatcca aaacatttgc tccatttgac aaagcaaatt gagagaacgc
    38401 ttgctcactc attgttcctt cagcagtagg catagcaatt ccatattgtt tattctcttt
    38461 atctgtgaaa tgttgagcaa tttttaaaat atcatcccaa ttttctggtt ctgaaaaacc
    38521 agcttctgat aatttttctt tgtcatacca aataccttga acccagccgc taattggtgc
    38581 agcaatatag ctttttccat cttctgaacg aacaaggttt ttggctccgt cataataatt
    38641 atcttctcca gcttcctcga taacgttttg gactgccgtc tgatcaatca gttcatcttt
    38701 atccatcact ttagcaaaat cctgactgac ttccattact tctggtaatt taccggaacg
    38761 agctaacgta acgatttttg tattgaacgc tgattcttct accggcactt gcttgatttt
    38821 gatgtctgga ttttcttttt caaaatcagc aaccagttca ttgatcacac ttaatcgttc
    38881 ctgttcgaca gaagagtgca tgaattgaat cgttgtctgt tgtgattcgt tagagctttg
    38941 actttcacca caagcagtaa gaagaaatcc agtagctagc agacaggtag taacaataat
    39001 ttttttcatt tttttccacc tcttatttta ttattttcag ccacatatat tcatacggct
    39061 ggaatatcaa ctttcgttct tgactgcaat ggataatttg tccagaaaac aggtctaggt
    39121 aatctccttt tggtaaaaga taacctgctg attgattgga aaaattatgg ataactaaga
    39181 ctttctcttc cttcgcttcg cgaataaaac tgataaaaga atctgctgag tccagtaagt
    39241 aaatcaaact attcggatgg aaggcttttt ctttttttcg aatagatagc atctgggtca
    39301 attcaccata tactgctgtt cttaatggat ctgtctgcaa ctctttctca atatctgcta
    39361 acaaaaattt ttgccgattg atttttcgat tctctcctgt tttttctacg cctttgtaat
    39421 catttcgact tcccagcaca ctttgaatat aaattgccgg cacgcctggt aacgttacca
    39481 aaaatgaatg agcagctaaa aagcgtttgc ttctccattc cgtcgtatct gttggagaac
    39541 ttaacgcatc tagatacgta gcgtttatct catatggact ggtcgttcca tctgggtttt
    39601 tcttgtagtt tactcgggca ccctctttct tcagctgttc cactaattca aggatcgttt
    39661 tttccggcag aatacctcgt accggattca atccgatacc gtcatgggag gctaaaaaat
    39721 taaaaaaagt tgtttttttc gatgcgaaat cgatggtttt taaccattct ttcaacttac
    39781 ttgtattacc cgaacgaatc gcttgaaggg tcaaaggtgg tagcgaaaac tgatagacca
    39841 tctgtgcttc attctcgcca ttcccaaaat aactgatatt atctttgtgt ggcacatttg
    39901 tctcagtgat caataccgtt cctggagctg cttcatcagc gattgcacgg aataattgga
    39961 tgattttatg cgtttctggc aggtgtatac aagaagttcc tagttctttc cataaaaatc
    40021 ccacagcgtc caagcgaata aatgaagcgc ctttttctaa gtaaaacaaa agaatatcga
    40081 tcattttcaa taaaacttct ggatttttaa aattcaagtc tacttgatct tcgctaaaag
    40141 tcgtccatac gtgtttttct ttcccgtttt tgtcaatgaa cgttgtaagt aacgggctgg
    40201 tccttggtct agtcacgctg cttgtatctg tatccagtgg caaatcaata aaaaaatctt
    40261 tgtacgtatc attcaagttt aaatattcct gaaaccaagt actttcggca gacatatgat
    40321 tgcagacaaa gtcgaacatt aaccgtgttt cgcttctcat ttcttctata tcttcccatg
    40381 tgcccaaacg gtcgttgatt gcctgataat ttacaacgga gaaaccatca tcagatgtat
    40441 atgggaaaaa tggcaagaaa tgtacaactc taaatgtatc tgccaaatac ttttgataaa
    40501 atttcttaaa tgtacgcaaa gttggttgct gctcttcaaa aaattgatcc ccataagtaa
    40561 tcaagaaaat atcttcttcg tcccaatgca gtttgcctgc ctggcctttc ttctgctgat
    40621 aaacactgac tttttgttca atcatagagg cggttttctc agacaaatct gagtcgcgat
    40681 aaatcgcttg taaaagtttt gtatacatcg gattcccctt tcttttttgg gagcgttccc
    40741 aacgatttga tcatattatg gaaaacgctt actgtcaata gcattttttt aaataatttt
    40801 gatatacaca aaaatacaaa taagaaagct tatgtatcaa taattaatag cttcttaaaa
    40861 aaatatggtc tctcatgatc atgcaagcta ttcctaaatt ttcaaaatca tagacaaaaa
    40921 agaatgtcta ttgtactatg gaattattct aaaacgctcc caaaaaggag gtgcatataa
    40981 aattgaaacc tactatttat gacattgcga aaaaagctaa tgtctcaaaa tcaactgtct
    41041 ccagagtatt aaataaccag ccaaatatct ctgataaagc aaaagcaagt gtacttcaag
    41101 caatcaaaga attgaattat gaaccgagca aaattgcaag aggactgtca ggaaatggtt
    41161 ttgacgcgat tctagcatta tctcatcgta cttctcatac gacctatggc aatccttttt
    41221 tttcagatgt tattcaatcc atttctgccg tttctgaaca aaataacttc gacttgattc
    41281 tacaaacggc aaaaacacca gaagaagagc tagaaaaatg cgcgaaaaag atccaagaaa
    41341 aaatgatccg aggaattatt attttaagct ctacgatgga tgaaacactt ctttttgaat
    41401 tggatcaata taatattcct attgtggtaa tcggaaaagt cggaaacgac tacacgaaca
    41461 tctactcagt ggacacggat aatttccagg atagttacga tttggtggaa acactgatca
    41521 agaatggaca ccgtcatatc ggctgtctcc atgctccgac agaagtttat gtatccaatg
    41581 atcgtctcaa tggttaccgt caatgcttat ttgatcacca cctagatatc cgtaatgaat
    41641 ggattataga cggcggcttt cgcttagaag atagcatggc agcaatgaat gatttaatga
    41701 agctacgaga aagaccaact gcgatttttt ctaccgatgc attaaaatca ttgagtattt
    41761 acaaagtggc agaacaatct ggaaagcaga tacctgatga tctttcgatc gcaggattca
    41821 gtgatccctt attgtcctcc ctattttcgc cacagttgac tcggatagac attccgacaa
    41881 atcagttagg aatcaaagcc acagaacttt tattccaact aatccaccac caataccctg
    41941 aagaaaaaag aatcattctt tcaaccgaga ttactctcac agattcgatc caacaacaag
    42001 aaatgtagaa cagaattata aaagaacaga aacactgagg ataagtcagt tactctgttc
    42061 ttttttcaat cactcatccc tcgttacccc ctcccggtat tttagcgaat ttttcttatt
    42121 ttccatctgt tttagatagc tgtggataac tataatgttt gtgttaaact attttttcca
    42181 cagggaagaa aaaactattt ttaaccacgt tgtgtataag tttttatagt tcgttttttt
    42241 aaacacattt acccttattt caatccatgt tatttgggga tacttatctc taaaacataa
    42301 atagaataac agacatgtct actggttttg ttttattcga ggaattattc aataatagct
    42361 agcaaaatat taaaaataac cgctctatct tcccatcaat ttattttgca ttcaaaaaat
    42421 gatctatctt tacttttgtt gattgacttc tcttggaaga tgatcgttcc gataccttaa
    42481 ttggatacga accgatgttg ttaagatcct ttcccacttt tcttctagtt agacaaatta
    42541 ttttagacaa accaaaaaag aggttgtgac aataatttgt cacaacctct tttttaaaat
    42601 atctattttg tttgattgtt cattgcgtct tctgcagcca tttgcgcttc aatatctgaa
    42661 atactgtaga cattttcgct tgctacaccg acttcttttg gttccatgtc acgataacgt
    42721 tgcataccag taccagctgg gatgatctta cctataataa cattttcttt caaaccaaga
    42781 agaggatctt tcttaccgcg gatcgcagca tccgtcaaga cacgagttgt ttcttggaat
    42841 gatgcagcag ataagaaact atttgtttct aatgatgctt ttgtgatacc tagtaagaca
    42901 ggtcgagaag ttgcaggaac gcctcctgag actagcgtat tgtagttttg atctttaaag
    42961 tctgcgatgt ctaataatgt acctggtaag atatctgtat cacctggatc cattacacga
    43021 actttacgca gcatttgacg aaccattact tcgatatgct tgtcgccgat ttctacccct
    43081 tgcatacggt aaacacgttg tacttcacga agcaagtagt tttcaacaga taatacatcg
    43141 cgaacttgca acaattgttt tggatcgatt gatccttctg tcaatggtgc accacggtgg
    43201 atcatatcgc cttctgctac tttcatacgt gctgtataag gaacagtata cgtacgtgta
    43261 tctgtttttc ctttgattgt tacttctttg gtacgtgttg caggatcttc tgagatatcg
    43321 atgacatccc ctgttacttc tgtgatcact gcttgccctt tcggattacg tgcttcaaag
    43381 atttcttgga cacgaggaag accttgagtg atatcgtctc cggcaacccc acctgtatgg
    43441 aatgtacgca tcgtcaactg agtaccaggt tcaccgattg attgagcggc gatcgtacca
    43501 actgcttctc cgacttcaac gtcagagcca gttgcaaggt tgcgtccgta acaatgtttg
    43561 cagacaccat gttttgtatt acatgtgaag acagaacgga ttgtcactgt ttcgacacct
    43621 gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt ggtttcctgc aatgatcaat
    43681 tcatttgttt caggatgacg tacttcttta cgggtgtaac gtcccactaa acgatcttct
    43741 aatgattcga tgatttcatt tccctcgcgg atggcttgga tttcaagacc tcggtcagta
    43801 ccgcaatctt cttcacggat gattacatct tgggccacgt caaccagacg acgagtcaag
    43861 taacctgaat cggcagtctt caaggctgta tcggtcatcc ctttacgagc cccgtgagtt
    43921 gagataaaca tttccaagac ggatagtcct tcacggaagt tcgaaatgat tggcaattcc
    43981 atgattcgac cattaggagc ggccatcaaa ccacgcattc cggcaagctg tgtaaagttg
    44041 gaaatgttac cacgagctcc agaatcagac atcatgaaga ttggattttt cgcttccaaa
    44101 ccttccatca gtttttgttg gatgctgtct tttgcagcat tccaaacagc aatgacacgt
    44161 tcataacgtt cgtcatctgt gatcagacca cgacggaatt gttttgtaat agtttcaact
    44221 tgtttatggg cattttcaat gatttcttgt ttttcattca gtacactgat gtcagcgatc
    44281 cctactgtga taccggcatg tgtcgaatgt ttgtacccta agtctttcat acggtcaagc
    44341 atcttagaag tttctgttac tttgaatcgt ttgaagactt ctgcgatgat gtttcctaag
    44401 tttttcttct tgaacggtaa tactaattct tgctctttga tatgtgcagg aatatcagta
    44461 ccagcttcaa caaagtattt gtccggtgtt tggacggtca agttgaagtc tgttggttca
    44521 ttcagataag ggaattcagg aggcatgatc tcgttgaaga tgatcttacc aactgttgtg
    44581 atcatcgtac gttctttttg ccattcagta aatggttttt cacctaatga gttagggttc
    44641 acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc atgcgataac ggcttcgttt
    44701 tgatctctga agaccattcc ttcgccttcg cgtccttctt cttccattgt caagtagtag
    44761 ttacctagaa ccatatcttg agaaggagta acaactggtt taccatcttt agggttcaag
    44821 atgttttgag cagctaacat caacatacgt gcttctgctt gtgcttcttc attcaatgga
    44881 acgtggacag ccatttggtc tccatcgaaa tcggcattat aagcttcaca tacaagtgga
    44941 tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga atgcttggat accaagtcgg
    45001 tgaagcgtag gtgcccggtt cagcaatact gggtgttcct tgattacgtc ttctaaaaca
    45061 tcccatactt cgtcttcttg acgttcgatt ttacgtttcg catttttgat gtttgaagct
    45121 agttcgcgtt gaacaagctc acgcatcacg aatggtttga ataattcaat ggccatttct
    45181 tttggaagtc cacattggta catcttcaag aaaggtccaa caacgataac ggaacgacct
    45241 gagtagtcga cacgtttacc tagcaagttt tgacggaaac gaccttgttt ccctttcaac
    45301 atatgagaaa gagatttcaa tgggcggtta cctggtccag taactggacg tccgcgacga
    45361 ccgttatcga tcaatgcatc tactgcttct tgcagcatac gtttttcatt ttgaacgatg
    45421 attccaggtg cattcaagtc taacaaacgt ttcaaacggt tattacggtt gattacacga
    45481 cggtaaagat cattcaagtc acttgttgca aaacgtccac cttctaattg aaccattggg
    45541 cgtaaatctg gtggaataac agggataacg tccataacca tccagcttgg ttgatttcct
    45601 gaagcgcgga atgcttctaa aatatccaaa cggcggattg cacgtgtacg tttttgtcct
    45661 tgtgcagatt tcaattcttc tttcaattcg gctgcttctg catctagatc aacattatca
    45721 agtaattgtt taatcgcttc tgcacccata gcagcttgga attcttgtcc atattgttca
    45781 cgtttttcgc gatattcgcg ttctgtcaac aattgttttt tctctaaatt cgtatttcct
    45841 ggttcgatga ctacatatga agcaaaataa atgatttctt ccaaagcgcg tgggctcatg
    45901 tctaatacaa gacccatacg tgaaggaatc cctttaaagt accagatatg tgaaacagga
    45961 gctgctaatt caatatgtcc catgcgttca cggcgaactt tcgaacgagt aacttccact
    46021 ccgcatcgat cacaaacgat tcctttataa cggatacgtt tgtattttcc gcaggcacat
    46081 tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca acccttcacg ttcaggtttt
    46141 aaggtacgat agttaatcgt ctcaggtttt tttacttcac cgtaagacca gcttctgatt
    46201 ttttcgggag aagccaaacc tatttgcata ctttcgaatt tatttacatc gatcaaaagg
    46261 ggttccctcc atttcattta atggcaaacc gcaagacggc tgatcttcga ccgccttgca
    46321 gctcatagac cggattagtc taaattatct tctgcggttt caaagttttg gatgacatcg
    46381 tctttttcat cttccacttg ttcagcagct ttcttttcta attctttggc agtttgctgt
    46441 tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt cgtcttcatc gtccatgtcg
    46501 cgaagttcga tctctgtttc ttcgatatcc aagacacgca tgtccagacc tagtgattgc
    46561 aattctttca ccaatacgcg gaatgattcc ggtacacctg gttttggaat tggttcaccc
    46621 ttgacgattg cttcgtatgt tttcacacgt ccgactacgt catctgattt gtatgtcaag
    46681 atttcttgta aagtataagc cgcaccgtaa gcttccagtg cccatacttc catttcacca
    46741 aaacgttgtc caccaaattg agctttacct cccaatggtt gttgtgtaac caatgagtaa
    46801 ggtccgattg aacgtgcatg caatttgtca tcgaccatgt gagcaagttt gatcatgtac
    46861 atcacaccga cagacacacg tccatcaaat ggttcacctg tacgtccgtc gtacagaaca
    46921 gttttcgcat cgctggccat accggcttca cgaactgttt cccatacgtc ttcatcactt
    46981 gcaccatcaa atactggcgt tgctacgtgg atacctaact gacgtgcagc catacccaag
    47041 tgtaattcca atacttgccc gatgttcata cgtgaaggca cccctaaagg attcagcatg
    47101 atatcgattg gtgttccatc aggtaggaat ggcatatctt cttccggcat aatacgggat
    47161 acaacccctt tatttccgtg acgaccggcc attttatccc cttcatggat tttacgtttt
    47221 tgaacgatat aaacacgaac taacatgttt acgcctggtg acaattcatc tccagcttca
    47281 cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc catgtggtac acgtaatgat
    47341 gtatcgcgga cttcacgagc tttttcgcca aagatcgcat gcaatagacg ttcttctgca
    47401 gataattctg ttaccccttt tggcgtgact ttccctacaa gcaaatcgcc atcttggact
    47461 tctgcaccaa tacggatgat acccatttcg tctaaatctt ttaatgcgtc ttccccgaca
    47521 ttagggattt cgcgtgtgat ttcttcaggt cctaattttg tatcgcgggc ttctgattca
    47581 tattcttcaa tatggataga agtgtatacg tcatctttta ccaaacgacg gctcatgatg
    47641 atcgcatctt catagttgta cccttcccaa gtcatgaaac caactaaaac gttttggcct
    47701 agagccattt cgccttgttc catagatggt ccgtctgcca gagtatcccc tttttcgact
    47761 ttctcaccca aatgaacgat tggacgttgg ttgtagcttg ttccggagtt tgaacgacgg
    47821 aattttgtta catcgtattt atctaatgca ccgttgtcgc ggcgtacgcg gatttcagaa
    47881 gcatcaacgt attcaacgac accgtcatgt ttacataaca aagcagctcc tgagtcatgc
    47941 gctgatttgt attccatacc agtaccaacc catggtgctt gaggattgat caacggcact
    48001 gcttgacgct gcatgttggc acccatcaag gcacggttgg agtcatcgtt ttccaagaaa
    48061 ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg atacgtccat gtagtctact
    48121 ttatcaatag atacttctaa gttttcactt tgagcacgag ccataacaac gtcttgagca
    48181 aatgttccat cttcattcaa tgggctgttt gcttgggcta cgatgtaatg gtcttcgata
    48241 tcagctgtca agtagtcgat ttggtcagta acacgacctg tttcacggtc tacacgacga
    48301 taaggtgttt cgataaatcc gaatttattt actttcgcat aactggacaa actattgatc
    48361 aacccgatgt ttgggccttc aggagtttcg atcggacaca tacggccata gtgggagtag
    48421 tgaacgtcac gaacttcata accggcgcgg tcacgagtca atccgccagg tcctaaggct
    48481 gaaagacgac gtttgtgtgt caattcgcca agagggtttg tttgatccat gaactgtgac
    48541 aactgagaag aaccaaagaa ttctttgata cttgccacaa ctggacggat attgatcaat
    48601 tgttgaggtg tcaatgtttc agtatcttgg atcgacattc tttcacgaac cacacgttcc
    48661 atacgtgcta gcccgatacg gaattggttt tgtaaaagtt caccgactga acggatacga
    48721 cgatttccta agtggtcgat atcatcgaca ttgccgatac cttccatcaa gttcaagaaa
    48781 tagctcattg aagcaatgat gtcggctgga cgaaccgttt ttacagctgc ttctggatag
    48841 ccgtttccga tcacgtttac ttcacgttca gggtctcttg gtgaaaatac tttgatgact
    48901 tggacagtca ttggatctgt gaccacaccg tcttctgatg gatagtaagt cactgaattt
    48961 aggccattat caatgaaagg agctagtgtt tccatgactt ggtgagtcag aactgttcct
    49021 ttttcaacga tgatctcgcc tgtttctgga tcaactaatg tttcagctaa tgtcagattc
    49081 aatagacgtg ttttcaaatc taattttttg tttactttgt aacgaccaac gtttgctaag
    49141 tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt tacgtgagct gtctgctgtt
    49201 tttggttcgc ctggacgcaa acgttcgtaa acgtctttca aaccttcttc tgttcttgag
    49261 tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc gtaatgtttc gctgtcgccg
    49321 aagatttcaa agatcgtatc atcggaacca aatcctaacg cacgaactaa tacggttaaa
    49381 ggaatcttac gtgtacggtc gatacgtaca taagagatgt cttttgcatc agtttccatt
    49441 tctagccaag ctccacggtt agggataaca gtagaaccga atccttcttt tccgtttttg
    49501 tcgacttttc catggaaata gacacctggt gaacggacta actgagaaac gattactcgc
    49561 tctgccccgt tgatgatgaa tgttccttgt tctgtcatta atgggaaatc accgaagaat
    49621 acttcttgtg ctttgatttc gccagtctcg cggtttgtca aacgcaatgt tacatgtaat
    49681 ggtgcagagt agtttgcatc atgcgcacga gcttctgcta ctgtatactt tggttctttc
    49741 aattcataat ctacaaattc caaagataga ttgccattga agtcatcgat tggcaaaatg
    49801 tcttcaaaca tttctctaag tccctcatct aagaaccatt ggtaagagtc tgtttgaatt
    49861 tcgatcaaat ttggtaattc caacacttca ctgattcttg cgaaacttct acgttcgcga
    49921 tgttttccgt attttactac gtgtccagcc aagctcttca cccctctaaa atgtcattca
    49981 aaaagactac ccgatcaatt gttacaataa tatttcaaat attattttta ggtaacaaca
    50041 gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat agaacattct cttgaaacct
    50101 tacttcgtga gcttctactt ttgtttcctt atattaaagc cgaaacggac aatatttcgt
    50161 ttctgccttt tcttcatcag atagtttttg caacttcata tactacactt attttctata
    50221 aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa cattttctta atgttttcaa
    50281 ttttatgttt tcaattatct gacaattctt aattgacaga cctttttatc tcggttatac
    50341 tttagaaaat tcattttgtg gaggaacttc tatgcgaacc aacatcaacc aaatcgcacc
    50401 aacacaactg aataactttt actttagcta ctttagatag gtttgtattc atcatgatgg
    50461 atacaaacgt taaacgacag tttgtatcta tgatggctaa agaactttgt attgcagcaa
    50521 aaagccacgt agacaaaatg ctaagtggct gagcagtgaa aatacaaagt aaaagtatca
    50581 agcattctac tttttcaatt tattttccga cttttcaatc catttggaca ttttgtctga
    50641 atggattttt tgtttcccaa aattaaggag gaaaaagaaa tgaaaaaaaa tataatcatc
    50701 ggattaggcg ctgccctgct actagcttta ggaggttgcg cacaaaaaga caattccgca
    50761 gaggcatcag atggacaaaa aacaattggc gtattgcaac ttgtacaaca taactcgtta
    50821 gatgctgcct ataaaggatt caaagaagga ttagcagaag aagggtataa agaaggcgat
    50881 aatctaacaa tcgattatca aaatgcccaa aacaatcaag acaacctgaa aagtatgagt
    50941 gagaaactcg tcaaagaaga tcctgatttg ctacttggaa tcgccactcc agcagcacaa
    51001 tctttattaa atgaaacaac cactatcccg atgactgtca cagcggtcac agacttggaa
    51061 caagcccatt tagtcacatc caatgaaaaa cctggcggca atgtgactgg cacgacagat
    51121 atggtgccaa tcgaaaaaca aatcggctta ctgttaaata tcgtgccaga cgccaaaaca
    51181 atcggcatca tgtataacaa cggtgaagca aactctaaga tccaagctga tttggcagaa
    51241 aaagaattga aaaaagcggg cgtcaaagtc aaagtactga cagcaaattc cacaaatgat
    51301 gtccaacaag tgacgaaaag tctagctaaa gatgtagatg gaatctatat tcctactgat
    51361 aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg caaaagaaac aaaaacacct
    51421 gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc tagcaacagt aggaatcgac
    51481 tacgaaagct tgggcaaaca aacagggatc atggcagcaa aaatcttaga cggcaagaat
    51541 ccaagtgaac ttcctgtcga aacagcgaat gatttggaac tgtatgtcaa taaagacatg
    51601 gccaaagcat taggcatcaa tccagacagc atcaaagaag ctgaataaaa cacaggagga
    51661 ttctcatgac aaatttgctc tctcttttct tatcatctgc atcgcaaggg gtcttatggg
    51721 ccctacttgc gattggtgta tacgtcactt tccgtatctt ggacattgcc gacttgactg
    51781 ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt aagtattaca aatggctatc
    51841 atccattgac agcttgtctt ctaggctttt tagggggtgc ggcagcaggg cttgtttccg
    51901 gactgcttca cacaaaattg aaaattcccg cccttcttgc agggatcatc accatgactg
    51961 gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa catctcttta ttgggtcagc
    52021 caacgatctt tcattgggtc gagtcattcg gcataacaaa agacaacgct gtactgatcg
    52081 tcggtctgct ttttgctttg atcgttgtat gtttacttgt tttgttctta cgtacagaaa
    52141 caggacttgc tattcgtgca actggcgata atctggcaat gagcgaagca aacgggatca
    52201 acacagataa tatgaagatc atcggttata tgatttctaa tggatgcatt gctctttctg
    52261 gctccttact tgcgcaaaac aacggctttg ctgatttaaa ttctggtatt ggaacgatcg
    52321 taattggact tgcttctatt atcatcgcag aagttttgtt acgtaaccag ccactatttc
    52381 tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg ttttatttta gcgttagtct
    52441 tcgaactgaa tgttgaacca agtgattcga aacttgcttc tgctttcgtg ctagtgatct
    52501 gtctttcttt cccgcagatc cagcaaaaat tccatttacc tcaaaaaaag aaaggcggta
    52561 gtgtcaaatg aatgacgtct taacattgga aaatctccat catacctttg aaaaaggaac
    52621 gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc ttagatcccg gcgacttcgt
    52681 gacgatcatc ggtggaaatg gggctggtaa atctactttg ctgaacagca tcgccggtac
    52741 attttctgtc gatcagggtc aaattttatt gaatggaaaa aatatcacga aaaaatctgt
    52801 tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat cctaaactag ggacagccgt
    52861 tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa cgaggaaaaa aacgcggctt
    52921 cttccgtggt gtcaaaccac aagaccgaac atttttcaaa gaccagctgg ttcggctaaa
    52981 tttggggttg gaaaatcgtt tatcctcaga aatcggtttg ctgtctggag gacaacgtca
    53041 agctattacg ttattgatgg ccacattaca gcgtccagaa ctgatcttat tagatgaaca
    53101 cactgcagcg cttgaccctg ctacttccaa aacggtgatg gaattgacag aaaagctgat
    53161 cactgagcag aatctcactg cttttatggt cactcatgac atggaagacg cgatccgcta
    53221 tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc gtagatatcc agcaagagga
    53281 aaaacagcag ctcacagtgc cccaattatt agcgttgttc aaacaaaaca gcggcacggc
    53341 attaaaagac gatcaagtac ttttatcttc ataatcagaa agaggatggg acaaaaaatg
    53401 aacgcccacc ctcttctttt ttctctttcc ttctttcgtt tttgctttat gtatcaaccg
    53461 ttgtttcttc gaaaactatt ttgttctctc cttcttcctg atagaatgga tgggaaagga
    53521 gcttgatgcc atgacaacga aagacaaggt acttcaatta ttgaaaaaga caccagattt
    53581 tctttccgga gaaaagttgg cacagcaact ggaagtttcc cggacaagta tttggaaagc
    53641 catcaaagaa ttggaaaagg caggctatcg ttttgagcac gggcctacag ggtatcgtta
    53701 cctgccctct gacgtactag atgcaaaaga aatccagcaa gagctacggt cgtttattcc
    53761 tgatttgaaa gtacaaacga tgttgacttc tgaatctacg atgaaagatg ccaaactggc
    53821 agcagctcaa ggagattctg tttcgaagtt atttgttgct gatacacaag aacaagctcg
    53881 tggacgtttt ggacggcctt ttttcgctga acctggacga ggaatctaca tgagtctctt
    53941 gctccatcca gacaaacggt ttgatgaatt gcctcaatat acgatcatcg cagccagtgc
    54001 ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct tcgatcaaat gggtcaatga
    54061 tatctacatg gatggaaaaa aagtctgtgg gattttatct gaagcagtca gtgatatgga
    54121 aagcggcagg atcagtcata tcatcattgg gatcgggctt aatttttctg ttcctcaaaa
    54181 tcattttcca gctgatctcc aagaaaaagc catctctgtc tttcccgacg gacaagctag
    54241 tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa ttgttcactt atctttctca
    54301 cccagaaaaa gcgttggact tctataaaga aaaatcattc gtattgggaa aaaccgtgtc
    54361 gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa gaaatcggtg atcatggcga
    54421 actgatcgtc caaacaaatg aacagaagat gagtttgtct tctggcgaaa tcagtctcac
    54481 atctatccgt acgcaataat tatcagattt cacaaaagga cacctgatct catacagtgt
    54541 ccttttttct gaattctctt caaaagactc tccttattct atcagctgac agtttctcta
    54601 tcctctagaa aaaggtattc taaaaatatc tataaagcat ggtacacttt ctttaaatga
    54661 atcaatgcaa aaaacaagag agtcatcagt ttacacgatc aaaagagata gcaggccgct
    54721 tcctttttca atcttataga tagaccatgg gtaacgataa aaacaaaaaa gaacttgcga
    54781 cttattttgc cgtaagttct tttttagcta gtgccttaat ctcttaatcc caacaaacgt
    54841 ctcgcataga tcacctgacc agagtgcata gcagcatcat caataataga aacgagacgc
    54901 attcctcgtg tgactggtgg tgtccagttc tcgtcaatta cttcatctag ctcggaaggt
    54961 tccagccttc ttaagtaaga aatagcaaaa tcagcagctc ctcgcaagta tcctagcaga
    55021 tcttctattt gattagcttg gatctgcttt gcttgttcca gactatgctt ccaatcttgt
    55081 tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt cttgactatg ccaaattgga
    55141 gaagttcctg ctaaatcagc aatctggaaa tctaattctc gagcggtatg ccaagccagc
    55201 caagtcattg atttgatctg cggggctcta tcttccactg gaaaacgatt ggcctcctca
    55261 atagagacgc cttcaaacaa acgttcaaac cgatcgtatg cccgatccac actctcgcat
    55321 agcatatcta aataatccat ttattttcct cctttttatt tatcctactt actttttcct
    55381 aattctagga tacctactta tatgttacta cacaccctta ttttttacac tttaacagtc
    55441 accatcatca atagtttgga gattgaaaga aaaagatagt gtcgaactat caattgaaca
    55501 agaagcaaca atatgaacgt ttcattaata ttctttaaat aaacaatgat ttttcaaacg
    55561 aacagtttaa gactaggaga aaaagagtat aataggaaaa tgtttgaaag gaaaggaagg
    55621 ggaaaaatga aaaaaagggc aagtttacta gtcctagttg ctttgttgtt caatatcttt
    55681 tcgtatggtg tggccgtttc agcggaaaca tacattccta aagaccctgg gaatatttat
    55741 aatgatctaa ctggtaacta tggcttgtta ggaattgcta gtcaattcca cgtatttgct
    55801 aaaaataaga caactgtgaa tgcacataca gacggaaata tagcaacaca tgaacttgat
    55861 gcaaaaaata attttggtac agagcttcat gaaggtttat tgaaccaaga aataaattat
    55921 atacaaaaaa ctgattcttt cgctaattct tcaggcatac ctacaggtag cacaagaatg
    55981 aataaatttg tcgttggcag taacgtggat gtagcacttc aagatggtca tcctacagtc
    56041 aatggatcca ggcttgatca cttaactcca gatgattttt ataaagatcg tgctgggcat
    56101 gaatatattg atttcgagca ggaatttgct aaattgaatg tagcagcaaa tgatcttgca
    56161 acgatcactc cagctaaaac atatactgct gcagattttc cagacatgaa caatcggaca
    56221 atcgatttaa ctgatttatc agataccggg tttttattgg taaatatcga tgcagaagtg
    56281 ttaacgatga atacgccact tcaaatcatt aatccaaatg atcaagtagt tgtatttaat
    56341 gtcatcaaca gttcttctgc attgaacgta cagtcaccaa tcaaatacaa tgatcgttca
    56401 aatcatgaaa cagaagattt ttcggatgcg aatatctctt ggaattttgg aaatgagatg
    56461 actgatttga caatcagtgc tccttttcaa ggaacaatcc ttgcgcctaa tgctactatc
    56521 cgcattactc aaaaccaaga cggaacgatt ataggaacaa atgtgatttt agatgcagct
    56581 accaatcgtt gggatcctaa cgaaatattt atcagcgaca atggtacaga tacgacagac
    56641 acaagcgaca gtacagatac atctaccaca agtgatagct cagattcatc aacttcaagc
    56701 gacagtacag atactacctc taccacaagt gatagcacgg atacatcagc ttcaagcgac
    56761 a
//
LOCUS       DAJSBM010000014        50462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJSBM010000014 DAJSBM010000000
VERSION     DAJSBM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.014"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            338..1141
                     /locus_tag="ONT67_000971"
     CDS             338..1141
                     /locus_tag="ONT67_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2354729.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            1128..1454
                     /locus_tag="ONT67_000972"
     CDS             1128..1454
                     /locus_tag="ONT67_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354730.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            1803..2843
                     /locus_tag="ONT67_000973"
     CDS             1803..2843
                     /locus_tag="ONT67_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2354731.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            3679..4011
                     /locus_tag="ONT67_000974"
     CDS             3679..4011
                     /locus_tag="ONT67_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2354732.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            4602..4931
                     /locus_tag="ONT67_000975"
     CDS             4602..4931
                     /locus_tag="ONT67_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354733.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            4918..5259
                     /locus_tag="ONT67_000976"
     CDS             4918..5259
                     /locus_tag="ONT67_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2354734.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            5373..5612
                     /locus_tag="ONT67_000977"
     CDS             5373..5612
                     /locus_tag="ONT67_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354735.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            5679..5918
                     /locus_tag="ONT67_000978"
     CDS             5679..5918
                     /locus_tag="ONT67_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354736.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            6004..6675
                     /locus_tag="ONT67_000979"
     CDS             6004..6675
                     /locus_tag="ONT67_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCR2354737.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            6728..8704
                     /locus_tag="ONT67_000980"
     CDS             6728..8704
                     /locus_tag="ONT67_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2354738.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            8717..9373
                     /locus_tag="ONT67_000981"
     CDS             8717..9373
                     /locus_tag="ONT67_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2354739.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            9469..10296
                     /locus_tag="ONT67_000982"
     CDS             9469..10296
                     /locus_tag="ONT67_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354740.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            10306..10554
                     /locus_tag="ONT67_000983"
     CDS             10306..10554
                     /locus_tag="ONT67_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354741.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            10597..12696
                     /locus_tag="ONT67_000984"
     CDS             10597..12696
                     /locus_tag="ONT67_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2354742.1"
                     /translation="MENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(12734..14791)
                     /locus_tag="ONT67_000985"
     CDS             complement(12734..14791)
                     /locus_tag="ONT67_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCR2354743.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(14905..15117)
                     /locus_tag="ONT67_000986"
     CDS             complement(14905..15117)
                     /locus_tag="ONT67_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354744.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            15304..15771
                     /locus_tag="ONT67_000987"
     CDS             15304..15771
                     /locus_tag="ONT67_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354745.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            15768..16010
                     /locus_tag="ONT67_000988"
     CDS             15768..16010
                     /locus_tag="ONT67_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCR2354746.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            16028..16330
                     /locus_tag="ONT67_000989"
     CDS             16028..16330
                     /locus_tag="ONT67_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354747.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            16401..18455
                     /locus_tag="ONT67_000990"
     CDS             16401..18455
                     /locus_tag="ONT67_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354748.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            18455..20287
                     /locus_tag="ONT67_000991"
     CDS             18455..20287
                     /locus_tag="ONT67_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCR2354749.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            21000..22898
                     /gene="ltrA"
                     /locus_tag="ONT67_000992"
     CDS             21000..22898
                     /gene="ltrA"
                     /locus_tag="ONT67_000992"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2354750.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            23015..23815
                     /locus_tag="ONT67_000993"
                     /pseudo
     CDS             23015..23815
                     /locus_tag="ONT67_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            23812..26343
                     /locus_tag="ONT67_000994"
     CDS             23812..26343
                     /locus_tag="ONT67_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354751.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            26393..26725
                     /locus_tag="ONT67_000995"
     CDS             26393..26725
                     /locus_tag="ONT67_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354752.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            26726..27319
                     /locus_tag="ONT67_000996"
     CDS             26726..27319
                     /locus_tag="ONT67_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354753.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            27482..29176
                     /locus_tag="ONT67_000997"
     CDS             27482..29176
                     /locus_tag="ONT67_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR2354754.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            29864..31741
                     /gene="ltrA"
                     /locus_tag="ONT67_000998"
     CDS             29864..31741
                     /gene="ltrA"
                     /locus_tag="ONT67_000998"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2354755.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            31872..32249
                     /locus_tag="ONT67_000999"
                     /pseudo
     CDS             31872..32249
                     /locus_tag="ONT67_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            32249..33454
                     /locus_tag="ONT67_001000"
     CDS             32249..33454
                     /locus_tag="ONT67_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2354756.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            33470..34114
                     /locus_tag="ONT67_001001"
     CDS             33470..34114
                     /locus_tag="ONT67_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354757.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            34125..34931
                     /locus_tag="ONT67_001002"
     CDS             34125..34931
                     /locus_tag="ONT67_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354758.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            34953..35183
                     /locus_tag="ONT67_001003"
     CDS             34953..35183
                     /locus_tag="ONT67_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354759.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            35729..36004
                     /locus_tag="ONT67_001004"
     CDS             35729..36004
                     /locus_tag="ONT67_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354760.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            36046..36468
                     /locus_tag="ONT67_001005"
     CDS             36046..36468
                     /locus_tag="ONT67_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354761.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            36487..38079
                     /locus_tag="ONT67_001006"
     CDS             36487..38079
                     /locus_tag="ONT67_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354762.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            38091..38426
                     /locus_tag="ONT67_001007"
     CDS             38091..38426
                     /locus_tag="ONT67_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354763.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            38573..38767
                     /locus_tag="ONT67_001008"
     CDS             38573..38767
                     /locus_tag="ONT67_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354764.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            38799..41171
                     /locus_tag="ONT67_001009"
     CDS             38799..41171
                     /locus_tag="ONT67_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354765.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            41232..42692
                     /locus_tag="ONT67_001010"
     CDS             41232..42692
                     /locus_tag="ONT67_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2354766.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            42703..44907
                     /locus_tag="ONT67_001011"
     CDS             42703..44907
                     /locus_tag="ONT67_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2354767.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            44923..45072
                     /locus_tag="ONT67_001012"
     CDS             44923..45072
                     /locus_tag="ONT67_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354768.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            45148..45741
                     /locus_tag="ONT67_001013"
     CDS             45148..45741
                     /locus_tag="ONT67_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2354769.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            45754..46653
                     /locus_tag="ONT67_001014"
     CDS             45754..46653
                     /locus_tag="ONT67_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2354770.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            46656..47141
                     /gene="ssb"
                     /locus_tag="ONT67_001015"
     CDS             46656..47141
                     /gene="ssb"
                     /locus_tag="ONT67_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2354771.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(47282..47485)
                     /locus_tag="ONT67_001016"
     CDS             complement(47282..47485)
                     /locus_tag="ONT67_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCR2354772.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            47560..47820
                     /locus_tag="ONT67_001017"
     CDS             47560..47820
                     /locus_tag="ONT67_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354773.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            48257..48463
                     /locus_tag="ONT67_001018"
     CDS             48257..48463
                     /locus_tag="ONT67_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCR2354774.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            48463..48714
                     /locus_tag="ONT67_001019"
     CDS             48463..48714
                     /locus_tag="ONT67_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354775.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            48729..49166
                     /locus_tag="ONT67_001020"
     CDS             48729..49166
                     /locus_tag="ONT67_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354776.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            49159..49866
                     /locus_tag="ONT67_001021"
     CDS             49159..49866
                     /locus_tag="ONT67_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354777.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            50247..50426
                     /locus_tag="ONT67_001022"
     CDS             50247..50426
                     /locus_tag="ONT67_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354778.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT        18695 a         7941 c        10206 g        13620 t
ORIGIN      
        1 tataatatta cgatatctta atatcatgat atcgtaatat tgtttttttc taagaaaatg
       61 cactataaga agggaattat tagtaacaat atagacagtt taataatgtt taaattattc
      121 tagttgctgc tcgcaaaaca tatttcttat gattaataaa aaattttaaa gtaataaaaa
      181 gtgattattt tactttcgaa taacatatga ataatcttta tatccgaacc gatatatatc
      241 tttaaaacaa aataatcaca caatattaca atataatgca tatacgatat tgcaatatca
      301 caatattatg atataattat attataatat aagatgaatg ggggatattt taatggcaag
      361 aatattgtca gtagccaatc aaaaaggtgg agtttctaaa actacgacta gcgttaattt
      421 agcagcatgt ttgaaatttg tttacggtaa aaatgtactt ttagtggaca ttgatcctca
      481 gggaaatgct acagataatg taggtttcga tatagatgga acaaaccaac ctacaattta
      541 tgaagtatta aaaggggagg ctgacattac agatgctatt ctggactata aaggaattga
      601 tgttttgcca gccgacatag ccctttcttc tgcagagagg gaatttactc aagtaggaag
      661 tgaacacagg cttaaaagag tacttcaacc aatcaaggac aactatgatt atattataat
      721 tgattgtcca ccaagtttag gaatgcttac agtcaacgca tttactgttt ctgatgaaat
      781 tatcattcca gttgaagctg cgtatttctc attaaaagga ctaataaagt taagtgaaac
      841 tattgaaacg gtaaaagaat atacgaacga caagcttgaa gtacgaggag tcctatttac
      901 taagtataat aagcgttata acatttctaa agaaatgacg actgcagcaa ccgaaatttc
      961 tagagtcatt ggtgcaaaaa ttttcaaaac ttatattcgt agaacaataa ccgttgacga
     1021 agcgcaagct gctggatcag atctaatcaa ttttaataaa ccctcaacag ctgaagatga
     1081 ctataaagca tttacagaag aatatttaaa agaagttggt gattttgatg gcagaagata
     1141 atcgcagtca agctgcagta agtagactac taggaaataa aaataaaaag aactacaaac
     1201 caaaagaagt agaagaaatc gaattaacca agaaaaatat aagtaaagtc ataaaagatc
     1261 aaggaagtag tgtaagaaca gcagatagca ttcgtataaa tccaattcta atgtcagcac
     1321 tcaaatattg gacgactatt gctgaatcag acaaaagtaa accagacgtt atagaagatg
     1381 cgctattaaa atccatacct gaagaatact tgattgaagg atataaacta gcaaagaaac
     1441 aaaataaaat ttaatttaca ttcaatatca cgatatcata atatcgtgat atttttttta
     1501 taaaaatcag tagtatcaaa gctgataagt cgcttatctt catattgtaa tattacgata
     1561 tcgtgatata atgttattgt aatatcgtga tatagttacc atgagcttaa accctgaaat
     1621 agagcggttt gctttaattt attttttaag gtaaactaaa aaaagataaa aaaagactac
     1681 tgctttaaca aagtttggcg acttatcagc agtagaaaaa tatatagtgg atagatatat
     1741 ttgtttaatt cattgtaaca tagttttgaa caaaacgcta tcctacagaa agaggattgg
     1801 ctatgaatga ttttaattat tacaaatcaa aagaaattta tcgtgaaaaa tattatcaaa
     1861 tgcctaaggt attttttact aacgaaaaat acatggatct ttcaaatgat gcaaaaattg
     1921 cttatatgct acttaaagat cgatttgatt attcagtcaa aaataactgg gtagattctg
     1981 atgataatat attcttcata ttcacagttg aagaattaat gaaattgtta caatgccgcg
     2041 aaggtaaagt atctaagatc aaaaaagaat tagaaaaagc aggtcttctg aaacagaaga
     2101 gaggaagagt aaataaacgt gatggtaaga ttgaatctat gcctaattta ttgtacttag
     2161 gcaagccaga agttacgaat caagacgtct ttaaaatcat ggaagaggaa gacaatactg
     2221 attccaccgt aattgcgaaa atcgcaaata cggcaaaacc gcgaaaaatc aatgattcta
     2281 ccgtaattgc gaaaatcgca aatacggcaa aatccagtca tatcaaggac tcaaccgtaa
     2341 ttgcgaaaat cgcagacaat ctattctatt ctaattctat agacactaat agacacataa
     2401 tagacactga aactgaccag ttacaaaatc aagttttgct agataacttc gtcgacatca
     2461 tgcaagagga cagtataaat acatttgtac cagaaaatgt attaaacttg ataaaaacat
     2521 tttcttcaag ttacagtgaa gctcaacaaa ccgtccaaac tattcataat gcaaagaaaa
     2581 aagctgaaga attagagcaa actattgtcg tttatgaaga actggaatct tatggaattg
     2641 atgctgacaa aggattatat atgactttac tgaaagctta tcaaaaacaa aaaactgaaa
     2701 aagtaaacaa tttgcagaat ctaattttcg tttatgtaaa aaattggttt gtcgaaaaag
     2761 ctattgcggc aaagcttgca aacgaacaaa gagaaacatt taacgaatta ccaacagtta
     2821 gtacggaaaa ttggttagag tagttgataa aaagcaatta ctataaaaat gatcaatttt
     2881 aaattagaac ttttttatga ttttaatttt attacaaata ttacaaataa atagttagta
     2941 ttccggagtt tttagattaa aagtttatga aaacaatcac aatcgtaggt tttaaattca
     3001 taatcgttag atattttagt tatttttcac tcaaaaaaat agggaactag tgtttgcata
     3061 tgtgcttttt tgagttataa taattcatga agagaggtaa ttcacttatt tccccaagct
     3121 gaaaagcctg atattacttc ttttcaggca gtgctgctaa tattcaatta cacttttttg
     3181 tatattttta gttgttttaa atttaattgt ctgctatgct tgtgatgaat tgattttcgt
     3241 tctgcacttt tggacgataa atttggacaa ttgaaaatac aaaaaaaaga aaggccctgt
     3301 gagactcgga aggctggtaa ccttcgtcga cgtccacagg tggcattaag atcactccct
     3361 taatgcgttt acctttcagt gttacggtat tacccgtaac ggatttagta taacagatat
     3421 tcaaaagtga taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg
     3481 aatctatgcc taagcataat gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa
     3541 cgctgaaatt agagggaacg catgtttgga tgcgttccct cttttttgtt tgtcatgaac
     3601 gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa tatttaaagt
     3661 aagtgggtgg attattttat gtcatataca ggaattttga gtctcgaaga tatttgtcac
     3721 tacggtaaac ggtgtaccgc tacagaaaag atcacgaaga aactatcaac tggtcaaagt
     3781 aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaagaaatg
     3841 atttattaca ttgggaaaca aaaacaaata attttaaaag atcctatccc tttaaaagag
     3901 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa
     3961 aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt taatcagctg aattgatcat
     4021 tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa
     4081 ggaacttaga atcactagaa aaataaggag tagacttgat ctatcaacaa ttcctttcgg
     4141 ggggtgacgg ggtaggtttt tacgattggt aacgtaaaaa attggtcaat cgagtaaatg
     4201 cccttgtttg gatagtggtg aaatgaattt gtgtaaataa tatgcacaga gtaaacaaga
     4261 gggaatttag aagttagaca agttagaata gcgcctagat taatcatgag cagctactaa
     4321 gggatctagg ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag
     4381 gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga agacgttgat
     4441 ctttggatca atcgagcatt taccgttttc tactagggtt tctcgtttcg gacgccataa
     4501 acgagtcgat ttattttata tatttttgag tgaacgaaaa acgataaaaa tattcgttat
     4561 gaagtaaaac taatttagta gatagcaaag gtggtagagg aatgattcaa aaatttgcga
     4621 aaatgagtaa aactgcatgg attgaaaagc ttgaaaatga cttgagtgtc atgcgattag
     4681 ctccaaagaa attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg
     4741 cggtagacga taaatgtcca gtaaaaatta gtttttcaga tatagcaaca attatcgaag
     4801 gtatttatta tggtgaagtt gatgaagaga tatcttctac aaaagattta ttagattttt
     4861 tggataaaaa caaagatacc tttatagtac taactgatta tgaggaggtt acaacaaatg
     4921 aaaaattatg atccgaatag ccgatgggga acacatacga ttaaggtttc tttccaacgt
     4981 tgggattaca aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt
     5041 ttagcattag acgaggatga tttatacgat caaaaattga ctgataatcc aattggattc
     5101 ggcttgctac ctgaagatga tcaaggtaat gaatggttta aaatgacgct tatgaatgat
     5161 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
     5221 gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
     5281 cagataaagg cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat
     5341 tgagtaagga atttatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg
     5401 atatggatac tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta
     5461 aagaagtatt caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag
     5521 aattcgatta ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa
     5581 ttaaacgaaa gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt
     5641 ttttattttt gcaaaaaaca aggaaggtag gaaaactcat gaattatgaa gaaatcgaaa
     5701 acagaaaaaa agtgtcgaaa gaaatggaag aaaagttgct aaaaacgatg aaacaaaaac
     5761 accttaaaag attatctgtc atgcaatata tcaatgatat gcagataaca ggaaaagaga
     5821 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gagacgaact tatgtgaaaa
     5881 aaagttctaa ttgtcaacta ttgttagaag tctcatgaca ttcattaata gaattcattt
     5941 ttaaaacttt ttctatggaa atgagaaaaa gattttttgt tttgtctgaa agggggtagc
     6001 tggatgagaa aatggttatt tggtttttta ggagtcgctt taatcgtctt ttgcagtgtg
     6061 tttggctatg ttagctacca aaaacatgaa ggaaaggtgt ttaagcaaaa tatagaaaaa
     6121 aagatgcccg tagatcaaat caatgaacat gcaaagagtt ataaggagga tgcaacgaac
     6181 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aaaaagaagc aatcgaaatg
     6241 ggtgtcaata aacaagcctt tgcacaaatt cagatacctg cacttggttt agcattgcca
     6301 atttttaaag gggcaaatca atacactttg agtcttggag cggctactta tttttatgaa
     6361 gatgccgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
     6421 gttttatttt ctgatattca aaagttgtca ttaggggagg tgatggactt agttagtaac
     6481 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgagta ctttaaatta
     6541 atcgatggcg ttcctgaaga gaactctttc ttgtctttac caaagaaagg agagaaaccc
     6601 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     6661 ggagagctac aataacaagg ttttaatcaa cgcgagtcgt tgaaatatga aatgaggaga
     6721 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttgttg gaatgttagg
     6781 aatcattgca agctgctttc aggggtatca agtacacgca gaagatgtca cgcaaaaaac
     6841 accaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
     6901 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
     6961 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     7021 ggatcaagta ttaccgagag aagattctga tctaaccaac gcgaaagtgg acgagattgt
     7081 acaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat
     7141 tacttcagct gtagatgaaa atggtgagat cacttttact gatcaaccag cctatgtcaa
     7201 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     7261 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat aaaacaggat caggattttt
     7321 aaaaaatatt catttgtatc ctaagaacat tgtttctaag ttgagttttg aattaacgaa
     7381 atttggtgac gatggcgcag cccaaagcaa acaaaccctt ttaaaagggg caaaatttga
     7441 gttatacaag ggcgaacctg gaaaaggaac aaaattgggt gatcttgtat cggatgatca
     7501 aggaaagctg actgcgactg atttgacttt aggaaaatac tattttgtgg aagttccttc
     7561 ggaagttgta gtgggatcag atcaagaacc aacagcagat caatatttat taggcgcaga
     7621 cgctcgcaac gatgcgcata ataaattaac ctttgaaatt accaatgacg gcgtgacaag
     7681 tgacttaaaa gcttcctacg tgaactataa agcaccagtg atcgataaaa cagtgacaaa
     7741 tggaacagga caagaacatt ctttccaaat tggtgacgca gtgaattatc aaggaacgat
     7801 tcatatccct acagatattg caggtggaac agatgggatt acagtaaatg gtgtgaagag
     7861 tgaaacttct ccttacagcg tatttaagtg gggagataca gcaggacaag gactttctta
     7921 tgtcgctgct aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga
     7981 aaatacagat tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa
     8041 taatggccaa gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta
     8101 taacatgtat gtaaatgaca gcgcagctgt tgccaatcca ttaacaaata gcgtggattt
     8161 cgtttataac aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     8221 tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa
     8281 agcaacaccg cttgaaggcg cagaatttgc tgctaaaaac gcagaaggaa aatattatgg
     8341 tggattagtt gatacggata aagacggtgt caaagaagca gtatgggtag acgatgtagc
     8401 aaatgcagcg atcctaaaat cagataaaga aggtcacttt gaaatcactg gtttaactga
     8461 aggtgaatat tcactggaag aaacaaaagc accaactggt taccaaaaat taacagaaga
     8521 tattgcattt aaagtaaaca aaaactcatt taaagaggaa aatcgaatca ctgttaaaaa
     8581 taatcaaaaa gcagctattc cattaacagg tagtaatggt ttccaaacgt atgtattgat
     8641 ctcttgcttg ttgctaggag caggggcact ttctgcagtt gtttacttta agaagaaagc
     8701 ctagaaggat cagctaatga ttattcgtaa gcccccaaaa agaagagtca tgcacaaact
     8761 gatgatattt ttttggttgc tgtcggcagt agcagtctat ttaattccag ctgtggaaac
     8821 aaatgtagcg aaatttcaac aacagcaaag aattttagcc attgaaaatc ttgaaaaaac
     8881 agattcttct ttggatgttg cgaaaataga gttaggcgat cctgttggtg ttcttacaat
     8941 tccaagtatt cctttaaaac taccaattta tgacggaact tccgacaaaa ttttagagaa
     9001 tggtgtaggg attacggaag gaactggcga tattacagga ggaaacggta aaaatccttt
     9061 aatcgcgggg cacagtggac tttataaaga taatcttttc gatgatttgc cttcagtaaa
     9121 aaaagggcaa aaattcttta ttaaggtaaa tgaagaacaa catgcctatc aaatttatcg
     9181 aattgaagaa gttaaagcag aagaattaca aaaaaattat ttagattatc tcatgcctga
     9241 aaaagaccaa gataggatca cgttaatgac atgcacgcca aaaggtgtta actcacaccg
     9301 ctttttagtg tatggtgaac gagtgtcatt cagtaaagaa gatttaaaac tacagaaaaa
     9361 agcaaaaaaa taggaaatca atttcattgg attggcgatt agcactgcta ttcgcttttt
     9421 ttgcgttcct attattttta tttataaaaa aataaggagg agatcggaat gaataagaga
     9481 aaatggctgt ttgtttgtgt agcagctctc tttgcaactg cagcttactc acagatatct
     9541 tttgctgata gcagtactac tccaactacc agtcagacaa cagatagttc aacaactgca
     9601 acaagtgaca cggacagttc gaagaacact gcagaagaaa cggcagattc aagtacgagt
     9661 tctactgaat cagcggaatc aacgtcagaa agttcttcag agacttcaga aaaactgcaa
     9721 gaagtagaag atacgcaagt aatcaagcaa aacattcata ttcaaaaaat tatttcttcg
     9781 aatcgaatca acaatcaagg aaggcaagtg actcagcaag aaggtgtcaa tggtgcaaag
     9841 tttattgtct acgatatcac gaatatctta gaagaaatga cacaagatga taatgagaaa
     9901 aatcaatcgg tagaaataat tgagtcaaaa ttgaaagatc gagcaaaaaa actttcatac
     9961 gatcagttga aaaaagtgac tgaaggagaa acccaaacaa ttgatgatca ggaaggcgtg
    10021 attgattttt caattgaagt acctgcaaac aaaaaacaag catactatat cgtcaatgaa
    10081 tcaagccctg aaaacatttc aaatagcgaa gatattgttt tattgacacc tgtttcagat
    10141 gaaaagggtg aatttttgac ggatgtttgg ttatatccta aatcaaagaa atcagagccg
    10201 aaagaagaaa ttaaaaaagt tgtatcaact ggtgtaaaga aaaacttttt tgataattgt
    10261 tgggattttt tcactcattt attttttaac gattaggatg aaatgatgaa aataataaaa
    10321 atgttagttt ttaatctatc ttttttagcc gcaacaatat tgtgttgcgg tttagttttt
    10381 tctcaattat caatttaccc aagatatcaa cgattgttcg ttcattttct aaatatccag
    10441 catgcttcca tgccactaga agtgattaac aagctcttaa tcaatccaat ttttatggct
    10501 agtttgtttc ttcttgcagg attggtaacg tgggtattag gaaaaaaaga atgagaagct
    10561 tacaatcgtt taacaaaata atagatggag ggcattatgg aaaataaaat tcattgcact
    10621 acgggaagag tatttacttt agtgttgttt ttgttccttc ttctttttgc aaggggaact
    10681 gcagtccagg ccttagaaaa agttgatccg agtcaaatca aagaaaataa taatctggta
    10741 ctttatcatg atagtgattc attgagaaat atcgaagtaa ctgggatcaa tggatttaag
    10801 atactagatg agtattcaac taaaaaacaa aaagtacttg cttggccaaa agtcaaagat
    10861 ttgaaaagca cgtcaaaaat tgttgcccat tatcaaaagg tgggcactta tctaggaaaa
    10921 gaaattgatt gcgacgtaac tttttctaat tttgtagcgc acgctggggt taaccaagac
    10981 ggattagcta aaacaattaa tgggaaaaag tattataatt atttgctctt tgataaaaac
    11041 atttatcgag gctttttcat gcagtcaatc aacgttatga atgttaaact agattttaag
    11101 tatgcaggat caaaaacaaa agtagctttg agtgaaccac agccagaaat taattcaaaa
    11161 atgagatcga tgagagcagc tggtattgag ttgaatgata catttctatc tatttacagt
    11221 ttaaatggac agtatcagca acgtgtgtct tggtataatg acaataatga atctgtcggt
    11281 gaacgtaata ttgatatgag agaatttgtt gggtacgaaa aaatggatcg cttaccttat
    11341 tttgtcacaa cagatacagc atgtgcagaa tacaaagctc ctgcgttatc aacaaacaat
    11401 ttaacttcaa aagtagtggg aggacgtgca gaaaaggctt atagctcgaa tgatcatttc
    11461 accgatgttg taggagctga tacttatcac agaagtggtg taacgtatac gcttcaaggc
    11521 gcttccccaa cattcatgat tggcgcaaat acgaatagta tgatgtttag ctttgatact
    11581 gcattgcttt ggacaccaca accatcgaag cctacaaaag aagtgtttaa caaagctaat
    11641 actgaagagg cagcacacaa tattgacaaa aaagtgattc cacaaggatc agatgtttac
    11701 tatcatattc accaaaagtt tgatgcatta acagtcaaca caatgaacaa atacaaatca
    11761 tttaaaatca ctgatacctt tgacagcaaa aattttgata tggtatcgga tgggaaaaac
    11821 tatgatggcg cattgtggta tgttgaagcg aatgggactt ggaaaaaagc tgatacagga
    11881 ggaaaattaa ctgtatcagg taatacagtc acttatactg cttctgacag ctttttagct
    11941 aatgctgcaa attacggtag ggactacatt ttgcagctcc atatgagagc aaaaaatgat
    12001 gcaaaaggaa ttgcaccaaa tgtggctact gtaacgttta acaatgatcc gaacaaagga
    12061 acacaaaaaa caaataaagt agaaaactat ttctctctta aaccaaataa aaaagtggaa
    12121 caagatggga aagatgttga tggtcgaaat gatggtaaaa aaggaacgcc tacagctcct
    12181 ttaaatgcag gatctgaagt acaatatcta gtcacacaaa aatggcatac aaaaggtgta
    12241 gacattgcgc tcgatcatta caagcaattt agtattcaag atccagtgga agctcgtttg
    12301 acgtataaag aaggttctgc gcgagtgata gatatgagta caggaaaaga tattacttca
    12361 gaaggtatac taacctatga tagccattca agaacgctga aatgggaagc ttccgctgat
    12421 ttcttaaata acaatctttt agatggacga gaaattcaac tgatatttac agctaaaact
    12481 cctttgaatg aagaattgga tatcgacaac caagcaaaag tttcagtaga aagtattgag
    12541 aaagcaacta atgtagtaac gattggggta aaacctaact tacctcaggt cattgtgcca
    12601 aaaactggat cgaatcattt agtgctaact gttattggaa gtatattatt tcttacttta
    12661 gcatcattgg gctatgtagt tttaaaactc aaataaatat aaaaacaagg ctgattttca
    12721 gccttgtttt cttttatttg aagattggat tcgcactaat aaatacatta tctacgccaa
    12781 ttctaccagc agagtcaact acaaaattat gacctaatcc gagaatactt tggttattat
    12841 aaagtaaatt tgcattatgg gcagggctat ttttccatcc taccatggca tcgatagcac
    12901 catcgtctga taaaaggtca tcaatataca gcccagtttg acttatgttt tctcccttag
    12961 caccatagca gcctaagctt tccaataaat cttggaaagt accaccacca gggcgatagt
    13021 gactgaagct gacttgtaat tctttagctc gaatatctgc agcctgttga acaatatcat
    13081 tgaattgcat tggggcaaca ccattttgtt ggcgataatc atttaaccaa gcaagttctt
    13141 tttgacgcat aaagttacgt aattcgtcta atgaaatctt tttatatttg aatgtgattg
    13201 ttttattttg atcgacgtta ttaatggatc gttggccgtt tttatccacg gtaacggtag
    13261 gatcgtcatt tctgcacaag aaataatttt gatcagtcat atttttccaa ggagtagaga
    13321 agctatcccc ttcgtatact gtttttgatt cactagagag aacagcgttg gtatcagcat
    13381 ttacatgatt aacagtcaca ttaaatttac gagtgtagtt gaaaatataa ttttgacttt
    13441 cagtcacatt gtttaaggtt actgaatttg ttggttctac ccgatcgtct aaaacatatc
    13501 cttgaataga aggagcagtt aaagtgacac tttcaccgtg agtgacgttg attatctgtt
    13561 ctttcaaaat atcaactcgc ccctcatttt tgtagttgtt attgcacaac aaattgattt
    13621 gatgcacttg attataattg aatgtgattg ttgtattacc tgtgattgtt ttgctttgag
    13681 tatcattacc aatcactttg tagctaaaat tatctttagc accttcgcta tatttaaatg
    13741 cttttgcctt agcagtgtaa gtagaaccag aagtcacttt tgcttgatct gtagcaatca
    13801 cttgacctgt ttgattgtca atatggttaa tggtaaccat atattctttt ggcgtttcag
    13861 gtttgtgttg caacccttta aatgcagcat caagactatt tttagcattg tttacttgtg
    13921 tttgagaaac cttagaatcg tctagtactt ttttagcatt ggataaagca gtctgaaaat
    13981 tattccaaga ttcttctgta aaatctcctt tagcagttga ttttactcgg ttgtataaag
    14041 cttgtaattc agctttattc acagtaggaa ctggtttttt ctttaatcct ttgtaagcag
    14101 aatctaattt atttttggca ctatccactt gcttttgagt cgcttttgag tcatttaaca
    14161 ctttcttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg gtatagtcac
    14221 cttttttcgt tcccttaacg ctcgtataaa gagcttctag agccgattta ttcactttgg
    14281 gtttagaggt gtcagcgcca taccaaccga taccttcgta tttccagcca tagtctttgg
    14341 ttaaccattg tacttctttc caacttgcag tgtagtgatg tgagccagat tttacaccag
    14401 gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccag gcaggccctt
    14461 cataagtcca gccgtaattt ttagtcaacc atttcacttc atcccaacta gctgtgtaat
    14521 ggtgatcacg aagaccttta ttgtacaaac gatagacaag atggccaccg ctagtcggtg
    14581 cttcccaggc ggggccttca taatttcccc attttatctt tactagatgg tcacgttctt
    14641 ttttgctaga ggtgtaaaaa tgttcattat tatttggatt gtaaaggcga tacatgacgt
    14701 tagttgaagc agcgtcaaca gatccagcaa gactaagtga ggcagcaaat gttccaatag
    14761 ctaaaacagt ttttggatta aataatttca ttttttcaac tcctaaattt aattaaaaat
    14821 gtttacatga ttattatata tcggttaacc gatatagtca ataaaaaata agaaaagcct
    14881 tatataattt tggcttttct tattttattt gttagttgaa gtattaatat caacgatttc
    14941 ttcagccaat ttatttaatt tcatagcatt tatgaatctc atgttgtcta tactagagcg
    15001 tttgttggca aggtcactta ctgtagttaa gggtacgcca gattctatgg atatagaacg
    15061 aactgattta ccactatcaa acagcaacca ttcgattttc tttttatcag cgtacatttc
    15121 aatgctcctt cttgtttttt ctttattata gctctttttt gtaatgaatc acaatatcaa
    15181 gagaaaaatt ttctatagaa aggtggattt ttatgagtat tcgttctccg tgtatccaaa
    15241 tgaggtttgt tgctactttc gttatttgat atactaaaaa taaacaacga aagaaggaat
    15301 aaaatgaaga aaaagcaact atggtttgtc tctatgtttg caatattttt tattatttta
    15361 agtggatgtt caagtaataa gaaatcggaa gctaaagaag ataatagttt attgcaacca
    15421 ggaactgttt ggaaagaaga agtaggtggt cttattcata atttaaaaat tatagatgat
    15481 actacctggg aatattctga aagtgtatgg catccagatc ctgtacaaat aacagttaag
    15541 aggcaaaaag actatgaagg tttagaaaga tacaaaatag tagattctgc aggcattagg
    15601 gaatttataa acaaacctga ttcattattt attgttgttc cctatgagaa ggatggcgta
    15661 aaaaaaataa tttttcttgg atctagcaag gatgagaagc gaacgaaaga gaaattaatt
    15721 catgatggat cacaatcaaa taaatacaaa ttacaaaaga ctagcgagtg attgctagtc
    15781 tttttatttg tcctgaagga ggaatcacag tggcaaaagc taaaacgcca tttgaaaaag
    15841 ccgcgtctaa aatcttagaa cgccgcggtg tatctgaaac acagtggaaa caagaagtcc
    15901 ttgaaaaagc acaatttgac ctattacggg gaaaagacaa ggacttagaa ggatatatct
    15961 atcaacgtga acgtgaaaag cttgttttcg atgaaattat taaacactaa aaggagcgaa
    16021 caattttatg tttgaatcaa ttacaatgaa ttttttaaca caaccgaaag tagtagctgc
    16081 ggttgatatt tttagagcat tacaaactgc cgcaactagt ttgaaaaata atctgatgat
    16141 ttttgctgct gttgcggcag tggcttccat tgctcttgga ggtattttct tcttctttgg
    16201 gcgaagagga gctgaaaacg ggaagacaca cgtaggttat gtattgttgg gggtaggagt
    16261 tattttagct gccgcttcta ttgtagtttc aatgttctca atgtttggac aaaccccaca
    16321 aggactataa aaaaacaagt agaaggagta aaaagtacgt ggctactttt tactcctttt
    16381 tttatggaag gagaatattc atgtttttta cgatttcagg caatgcaaaa ttggataaag
    16441 agttaataag aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa
    16501 ttaaatattc tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac
    16561 aagagaatca atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag
    16621 aaaaggctgg cacagaatac gtttttggct cactggatta tcaaatttca gaaggttttt
    16681 atcctacatt gattgattta atccgcctag atatatttaa agatacggat gaaaccatga
    16741 gtgtggaaga gtcttatcga aacgaaaaaa ttttacaagt tttagaagaa gagttagaaa
    16801 gcgaccaaag aaaagtgaat caagtgagtg aagtcgaagc aaacgaagaa gtaccatggg
    16861 aacgcccaat ctttccaaag gatgaatcaa aagaaaaaga gaaaggaatt attgacgaac
    16921 aagctgacat tttgactcat aaatcaatat ttgaggaaga gaacgaggta atgggtcaat
    16981 tggaggtaga tccctttgaa gaaacaatgg aggatgaaga gacggccgaa gtagaagttg
    17041 atccagttga agctaatgaa gacccaacat ggactgaagg ggattctcag gaatatattg
    17101 aaccacttat cagtgaagaa gtagagaaaa atgaaacaga gatacaaaag gcaacggtat
    17161 ttgattatat tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaataaat
    17221 tatttttaaa tgaactttat tcgaaatatc aattacctca aacctatcaa gcatttttag
    17281 agagtaaaga agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg
    17341 tccaatcaac aaattggcat gagcttgcac aagaagattt attagctact tttgaagaac
    17401 gtcaacaaga aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tcgaattggg
    17461 agaaagcaaa agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa
    17521 aaattaaaga gcggtacgaa aagaaacgtg acaatgcgta ttccttgtgt gtagaaaaga
    17581 tcaacaattt tactcagacg aatcaagaag cattatcgaa agaaaaagaa aacttattaa
    17641 aagaacgtat aaaagaacga aaagaagaat tttaccgagg gttgcgtact gataagctaa
    17701 ggattagcaa agaattaaat gaacaactga ataaactcta tcaaactacg actgaagttc
    17761 tgatggaaaa acacgagcag atccaacaag aattacaagc acaaattcca aaatggaaaa
    17821 aagagtatga aattcaccaa caagaattga aagaacaaga agaaaaacaa cggaaaaaag
    17881 aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca ggatcttgcg gaacaaaaaa
    17941 tggcgataga aaaacaacgg ctagaacaag aaaaggaaaa agaagcagct gctcgtaagg
    18001 aacgtcaaga acaaatggat acattgcgac aagcacagtt aaattatctg acacatcctc
    18061 aaccagtagc tgctgttcaa acgccagtga acaaccaagc aaatttaact ccttcacccc
    18121 agcgttcctt taacggatgg atggtgggat gtattgcaag tttaagcttg cttctaggtg
    18181 gaggaacagt atttgctttt aatcatatca atcaatcttc acaggctgat tctgtagcca
    18241 caacggcgtc aatcaaggaa gaagcccagg aacaagcaag aaaagagtat gaacaacaat
    18301 tagaagaatt attgaatcgt accaatcagt caacaacaga gtcaagcatg gaaaatagca
    18361 aagagagtga ttctagtaag gctaaagaag gatcaagcga ttcaactgta caatcaaaca
    18421 cttccgaaag ttcagaatca aatgaggtga aataatgcgt gttagagaaa caacgaagcg
    18481 aaaagacaaa cgattcaatt tagaaaagaa taggtatctt tctcatttct atcgctatac
    18541 tccctcagga gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa agatttttta
    18601 tagtggactt gtatacctcc ctttagagta tttagtaaca agagtaccag aagttttcaa
    18661 ccgattggaa attgttaagg gaagcttaca aggaataaat ctgtttcaat tacaggaaat
    18721 gccacaggtc tttcattttg attcagctgg tttttggaat ccatttactc ctacaactag
    18781 tttgggtaca cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt
    18841 gaaaatggac atgtggtttc gtcctctagc agatggacaa aaaggggata atcgattaat
    18901 gaccttaaaa gaaatccaac agatttatcc gcaaatacct gaaacaaaaa ctacttttcc
    18961 aggttatgga ggtattccaa tcacacatta caaaaaatat tggtacattc aaacagatac
    19021 tgttaatacg tgtattgttg gtacctctcg atcagggaaa ggtcagattg aagtgttagc
    19081 caccattgac aatttatctc gtgcagaaaa acaaagttca atggtcgtta atgatccaaa
    19141 aacagaatta tatgttggta gtaaagatga actagaagct agagggtatg atgtatatgc
    19201 ctttaatatc accgatccac tgcaatcaat gagtgataat ccattggcac tcatcgtgaa
    19261 atactggaaa cgaggagaca tagatactgc aacacagtta accaatactt tcacaaatac
    19321 gatttattat gatgccaacg cttcagataa caaatacttt aacgaaaacg cgcagaaagc
    19381 tgtcaatgcg cttatttttg cgttgttaga acaagctgat caaacaggag attactccaa
    19441 attgacaccg aataatctcg ttgagcttct ttctgagatt ggcggtttta attattatcc
    19501 agatccagac aatgaattca aacagttgaa tgccttagat gaatttatga atcagttgcc
    19561 acctggaaac attgcaaaaa aacaatatgg ggcaacgaaa attggatctg ataaagcgaa
    19621 gggaaatatt ttatcaacgg caatcacggg attgaatcca tttactctaa cgaaaattgc
    19681 aaagatgact tctcaatcaa cgattgatta taaatcggtt gggtttccaa aatacttaga
    19741 tttgaaatta catgaaagtc ttttaaatca acgagtcgta gtagaatttt atcataaggg
    19801 aaagaaattt catgaagaac gtgtcaaagt aggctataaa ggatttagtg aaatcaattt
    19861 tgattgtcat ttagttgcag gtgatcaagt ttgtttaaaa tacgatcatc aaggaaaaga
    19921 atatgtggct tggtatgaat tttctcaaag agtattgaaa gacgaagagg gtaatatcgt
    19981 ttatcggaaa aaacgaggag aaacccattt gccagaatta gaaaaagaag ccgttctaac
    20041 attagatgag gaaaaaagta acttattagt agagtctatt cggatgcact ataccgataa
    20101 accaatggct atttttatgc ttacacctga ttatgatcca tctaaccata tcattgttag
    20161 tatttttctt agtcagttat ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga
    20221 taaatgtcac cgcaggattc attttatttt agatgaattt gggaatagtg tgacctgttt
    20281 agcctaagga taaaagaggc tagttccaac gaacattgga ataactttac tatcgagaaa
    20341 tcaaatgacg gggtaacgcc ctgaagggtt tccactgata ctgcgacatg cgttagtagg
    20401 atgtgcatcc aaacgcgtat gaagctcgct gaagtcggca gaagggtacc gtaaggaaag
    20461 acacgatacg tatctaccac gcaagtgctc tagaggtagg gtatgtacct gatatgtcgg
    20521 gtgtcctaaa aagttcaata aggtgagaat gatttgatta tctgacaaac ttgcgaatgg
    20581 aatgtctgaa acgaataact catagaaatg tgagggggaa gggggatacg tcctctttcc
    20641 attgactcga ggttgagtaa aaatgtgttt ctatgaaaaa ccttgcatgg ttaaaggcta
    20701 tggagtagtc aacaggcaaa agcaagcacc taagttgaaa tctatggata gatagtaagg
    20761 aacaggggaa gcatctagca cggagggagc ataccgaaga tgtgtttcta aggaaaaacc
    20821 gaaatcggtt ataacttagg tgaatagatg tgaaagtaga gccacgacca gtgaaaattt
    20881 cgtaatgaaa ttggaggaac aggctcaagt cgttatttat aatataagaa acctatttaa
    20941 aatcatggat tctcgtaaga ctaaaaaggt cacgaaccag aatggagcgt gataaaacca
    21001 tgactaacac aaaaggaatt agcttaaaga acaaacaact cagacacgcc gaatactacg
    21061 acatgactga gactttcgat gaattgcacg caatgagtgt tgcaaaatca aattttaaaa
    21121 atctcatgaa attcattatt gatgacaata atatctttct agcgtataga aatatcaaaa
    21181 agaataaagg aagtgcaacg gccggatacg atggtgtcac tataaaagac attgaaaaat
    21241 tggcatgtgg tgacttttta actattatta aaaggcgatt taaggattac agaccaagaa
    21301 aagttaaaag agtagagatc ccgaaaccaa acggaaaact tcgaccttta ggaattcctt
    21361 caatatggga caggatagtc cagcaatgta ttctacaaat tttagaacca atatgcgaag
    21421 caaaatttaa taagcatagc tatggattca gaccaaattg ttctacagaa aacgccatag
    21481 cagattgtct tgctagaatc aatcaaagta agatgaatta tgttgtagat attgatataa
    21541 aaggattctt tgacgaagta aaccatacca aacttatgcg tcaaatatgg acaatgggga
    21601 ttcgagataa acaactcctt gtaattattc gtaagatgtt aaaggctccg attgttatgc
    21661 gaaatggcaa tgttgttttt ccaaccaagg gtactccaca aggagggatt ttaagtccac
    21721 tgcttgcgaa tatatgcctg aatgaatttg attggtggat agctaatcaa tgggaggaac
    21781 gaactgtcaa agagctgaaa caccactatc gaaaaaacgg agcatgggat aaaggaaata
    21841 tctattcgaa attaagaagc cacacaagtt taaaagagat gtatctggta cgctatgcag
    21901 atgattttaa aattttctgc cgaacaggag aagaagccca aagggctttt tatgccacac
    21961 aaaaatggct cagagaaaga ttaaagctcc caatttcaat ggagaaatct aaagttacga
    22021 acctaaataa agctgaaagt gaattcttgg gtttttcaat aagactccac aaaaagggaa
    22081 aaaagaaagt ggctttcacg tacgtttgtc gcaaagcaga agaaaacatt aaaaataaac
    22141 taaaacaaca gattaaaaaa attcaatacg ctggagcaaa aaaaaatagg caccgagcaa
    22201 taggtgacta caacatgatg gtcatcggta tccacaatta ctatcggata gcttcacatt
    22261 gtagtgtcaa tttcactgag atttatagaa gtgttaggac agtaatagaa gcaagactga
    22321 aagctagagg aatgacgaaa aatggaatct atggacaaga tcatgaagga taccgtccgt
    22381 atctaaagtc taagcaaatg agatatattg aaggttttcc gatacttcca attggctata
    22441 ttaaaaaaag aactccactc aacaagcgga gaaatctaaa taaatatacc aaggaaggga
    22501 gaaagctcat tcatcaagag caacaagcta tagaaagctg gaaaatcaag tatcttgtaa
    22561 aaggttttag taacaataag aaatacacgg tggaattttc tgataacaaa atatctaagt
    22621 ttatttccca aaaagggaaa tgcgctatca caggaaacga acttttgatg gaagaaattg
    22681 aatgccatca cattaaccca tatcatttgt ccaaagacga tagttacaag aaccttgtca
    22741 ttatctctag agaaatccat agattaatcc atgcaactag tgaagaaact attctgaaac
    22801 tgcttcggtt actgaaacta aatcactatc aaattagaaa actaaacacg tatcgaaaaa
    22861 aagcagctaa ttttccaata tcaatcaaaa tgaattagat aggaaacaaa ttatattgta
    22921 attaacgatg gaacgccgtg tgcggtgaaa gtcgcacgca cggtgtggac cgggggaaaa
    22981 gacggagata atatcaaact cttacctatc ggaattgcca ccaatggata atatggaagg
    23041 aatcatgacg gtcacagctg gccgaaacat gttgtgggat ctgtttattc aatcttatca
    23101 gcaactttat gcgaagtatg gagaacaaaa cgcagcgatt atcaaagaaa attgtcagac
    23161 acatatttat attatgtcga cagatgataa taccatcgaa gaattttcta aaaaagtggg
    23221 gaataagacg gttgaacaag agaacagtac catcaattca ttaggtatga atactcatat
    23281 caatcgcaat gttgattcag atcgtatctt aactcctgaa agaatttcta cttttttaga
    23341 aggtgaaacg attgtactcg ctcctttaaa acgacaagat ttgaaaggtc ataaaatacg
    23401 gccgtatcct atttttaatc atggggaaac gaatcttcct tatgcttatc aatatattga
    23461 agatttcaat ccatcgtggg atatcaatga tatagacata cattgtacac atgcggactt
    23521 ggatcttaag gctttacaaa ttgattatga ttatttccta ccagatgaat atgctagaaa
    23581 tctctatcat ggactgcatg atcaattaga agaaaaaaac gaagaaaata tgttttataa
    23641 aatcgttcaa tctcttgaga atgagacggc taaaaaattg ttaattcaat tttacgaaga
    23701 acaagcagca gatcgacttc aagaatatgt tgaaaagttg atcaaaaatg aagaaatttc
    23761 tgaaatgatt gggaaacggt tgttattgga atctgaaaag gtggtgaaag aatgatcatt
    23821 ggaggaattt tttctggcat agccgactgg tggtcaggct tattcggaga aggggatgca
    23881 aaccccaaaa ccgtaacgaa agtcttacaa gattatggta attatctgca gtatcaagat
    23941 gtgttcagtt ggatttgtaa tactttttta tgggggttga tcaaagggtt atataatctc
    24001 aatactttac tagaaaaaac catttatcaa agctttgatt taaaagactt attgaacgcc
    24061 gctggggtaa atgaactatt tcaaagtttg attagtaagg tattggctct tttttgtgtc
    24121 gtcacattaa tttatttagg cttgaaattt tttatgagta aacacccacc aaaagtcaaa
    24181 aatatcttgg ttaatttatt tatggccatg atcttgattt ttggtggtag ttctttaatc
    24241 gatgaagggt taaatatcag taaaagcttt tacggagacg tcactgcagc aaataaagca
    24301 gataaaacgg gttcacctgc ttttcaaatt attcaaaata atgtgatcga tgtcagtaca
    24361 ttactagcaa ctgacccgaa taaagtagaa tccctgccaa cagacaaaag aaatgcttta
    24421 acagcgaaaa attttaaaac agcgaatatc aatgggatta ttactcctaa acaagctaaa
    24481 gaaatggcgg ataaagataa gagtggttta tcagatttac aaaaggatcg gttaagagcg
    24541 ttacaatacc gactggaact ggatgataat ggaaaagaaa ttcctgtgca aatcagtgat
    24601 gaaggcttag ccaaatatgt ttatacttca gggtatcgtc gatacattag tagtccaggg
    24661 attattttag ctggtgaatc ttctttggcg gtggcctatt tatttatcct atttacgatc
    24721 gttacttgca ttattgagct ggtatttaaa aaattctatt tagtgattgc cgcgtcaacg
    24781 gactttgaga caggacaacg gatgaaaagt gctatcgaag atatttctca atccttcttg
    24841 ttattggcat ttacaatttt agaattacga atctatacct taatgctttc aggtattggt
    24901 gatttgcatg cggcaggaaa gatcaatgga ttcttgtatg ttgtcgcttt gattgtacta
    24961 accattgcgc tattcaaagg ctctcaatca gttacgaaaa ttttcggcgt tgatacgagt
    25021 ctgaaaaacg gcagtaattc tcttcttagc atgtttgcct taggaaatat tgcgaaaaat
    25081 gtgggtagcg gagctaaaaa ccttgcaagt gctggtaaga gtggtttaag ccatttaaat
    25141 gatattcgta aaaatggatt tggccatcga aaagatgtta cccctgataa cggggaagaa
    25201 caatcagacg ataaagctac aactgtacct aatagtaaga aaacagtttc cgaaatgttc
    25261 gggaaagcga aaaatggtgt ttctaaagcc gcagaaggtc ttggttacgt caatgaacgt
    25321 gggttaaaag gaacagctga agatcttaca gaaaaagcca aagacacctt agaagataaa
    25381 ttagacggca cgagagctaa agaattggct gaagctgtga agaatccatc cgaagcacta
    25441 gaaaaacaaa aagaaaaagc ttcagaagtc aaagaaggac taaaagatgc gtatggtgaa
    25501 ggacaagtca atgcagcagt gaaagctaat acaacaaccg aaaaagaaaa tcaagaaggt
    25561 atagtgattc cttcagtcaa agatcaccaa gaagcgttgc actctaatga aaattcgatt
    25621 gaagagaaaa attccataga agaaagaaat tcagaagaag gaaaaaatgg acagttacca
    25681 aaaactgatc cagaacaata tcgctataaa cctaacagtg accttgaaaa tgagaagcga
    25741 atttcagatt tatcaaaagt aaatcaagaa gacaaagcag aaaatgaggg acagttacca
    25801 aaaactgatc cagaacaata tcggtataga tctgttaact ccgaaaatga aaaacgcatt
    25861 tcagatttac caaaacgaaa tcaagaagta caaacggatg aggattccat cgttctgcca
    25921 aatgataatg gaatcgaagt tggaactgaa aaatcaacga aaattggttt acctaaggca
    25981 caaaatttac caatgaaaga tagtcgtttg aatcaattca aggataatga tcaaagtttt
    26041 gttcatcaat cacctgcagg tactgttcaa atgaaaccaa gtcgtttgcc tgaaaatgga
    26101 aatctgacaa ctgtttctta caatttatct gaaagtggtg agacagggaa tgcgtccgtt
    26161 caaaatgttg tgacaagaga agctgcacca aaacctattc aaaacgttca tacaggttac
    26221 aagccagcaa atgtggcatt tactgcacca acaaaaataa atgtgacaag agaaactaaa
    26281 aacaggaaca atgcattgaa agaacaacta ttgagggata tcaaacagga tatcaaacca
    26341 taaaaggcct acaagttagg ccttttcttt atatgaaaag gagaaatcaa ttatggataa
    26401 atatataaac atgaaaaacc ctccaagatt tagtggagaa tggaagtttt tcgctttttt
    26461 ctttttagat ctattaacgc ttggtggctg ctttttttgg gcgaaaatgg tcaataatct
    26521 tgtccactta ccactctttt ttgcactctt gaattatggc ttatggattg cgatagcgtt
    26581 agtgattgtt tggcgaccct ataataaccc aggtcaacga caaatttttg cgattatcga
    26641 gtctattttt tttgcggaca agaaccatta tcatgcgtta gatcctaacc gaaattatca
    26701 tggtacgtat aaggaggatg aataaatgcg agtcaaagga caaaattcag ttgaaaggaa
    26761 atccattttg cagtttggaa aaccagaaga gcgctctatc ttggacttat ttccgtataa
    26821 atcctgtaca gaagagggag tcttaattac tagagaagat cattttcaac gttattatcg
    26881 tgtagtttct acagacgtgg aaggattaaa tgaacaagag aaactagaac gaatgaatca
    26941 gttgaccacc gtcatgcgta cgtatgtgcc aacaattaag ttagttagtt tgaccacaga
    27001 gacggatcta acggaacaaa tcgtgcaaaa gcggcagtta ttagataaaa atcgtatgga
    27061 acagtcctta ggaagaaatc tacagcgatt aagaaaatat gaaaagaaat tagtagagga
    27121 aatccaggaa ttgaaacgag cagaacagga acgccctgat ttaagcttct ttttcgtcat
    27181 tgaaggaaag acaatcaagg atttgcaaac cagaaaccgt cagttaatgc gatctagtgg
    27241 cattttaggg ttaaaaccac tctacaaaaa agaattgatc aagattcttt atcgaatgaa
    27301 taatatgaat gatgagtagg aatggggaag agagaatgaa atatccgttg atatggttag
    27361 aaaaagatgt tccgaaagaa gagaccacta gaaaatttcc tcacacttta agcctgggta
    27421 agttgggaaa aggaaaatct tttaaccaag taaagaatca aacaagaaga ggtgactgta
    27481 catgttagaa gcaattaaaa cagctttttc accacaaaat gatcaaaagg tacttgatcc
    27541 attaaagcaa ctaggaaatc gggaaataga aattttaaaa tccaaagggt atgatttaga
    27601 ctttttaaaa caagtgatgc cacaaggtgg attaaatttc gatgaagagt atgtgaccta
    27661 tggaaatggt tattcacgat gtgtgacagt ttatcactac acaaaaaatc ctacgctttt
    27721 ttggttggcc aatctcatga acaatcctca tacagtggca acactagatg ttagcacaga
    27781 tgaaaagcaa aaagtgattg atcaaatcaa tcgtgcctta gatgaattaa atgatcggtc
    27841 agataatgaa cgaaaagcga ctgaccgaaa taaaagcgcg gaagaatacc tagaactagc
    27901 ggaatatgcc cgaaaattga cttctaatgg ggaaatttct aaacaagtca aagtgagagt
    27961 acatgtttac ggaaacacga ttcaagaagt agatgaacga gcacaggaac tgatcgaaaa
    28021 tctcaaaggg atggattatc gtgcagtgac ctatctgcac aaaacgaaaa gagactggca
    28081 atcgatgttc accatgttcg atgaacaagc tgaatggttt ggaaatatca aagggcaatc
    28141 actaccatcg ctaaacattg gcggaggttt cccgttccac caccaaacgc taaaagatcc
    28201 aggtggaaca caatatgggg aaaccatgac aggcggcccg tttgtttgga atgccacgta
    28261 taaaaatagt gtgcggttgt ctaccaatac cttgcttcta ggaatgatgg gagctggtaa
    28321 atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg
    28381 ctttgaaaag ggaaaagact ttattccctt tttaaaggaa tataacggca ttatggtgcg
    28441 tttagacgga tctgacggga tgatcaatcc tttagaaata tttgccacac gtacgtatga
    28501 tgaagcttcc agtttatatg acgatggatc agtcaaagat ttgaagatca atgaagcagc
    28561 gtcctatcaa acgcacttag acaaagtggt ctatcaggta cagttagtga gtcctcagct
    28621 aaaaggaacg atgaaagcag aatttaagac ctatttaaat cgtttttatg aagagtatgg
    28681 aacagtacca cgcggcttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac
    28741 aggtaaagat ccagaagctt atccgacgtt caaagaattt ttagactttt taggacaact
    28801 ggaattacca ggtgcttccc aagagaaaaa gaatcgaaaa gaagaaatgg aatccatcgt
    28861 agaatcctta tgtgagacgt atggcatgat ttttgatggt catagtacga ttcgacacct
    28921 tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcttag atcgaggggt
    28981 acaacaatgt caaatgtaca tggctatgac gttgatttgg aatcaggcat tgattcaagg
    29041 aagaagacaa cgacgtttaa ttaaagaaaa acaaatggaa gaacaagact tcaaatgctt
    29101 cttggcgatg attgatgagt gccataatgg tgcgacctgt tctgcagtga aaagctgtgg
    29161 gttgcaagta gtctaaaaac tattgtaata actcttataa cgagaagtca aatgagtggg
    29221 taacgccaca aagggcttcc actgatactc cgacatgcaa caagtagttt atgcagacca
    29281 acgttgtgtg aagctcggtg aagtcggctg aaggataccg taaggtaaaa gaaatagtga
    29341 ataccacgaa agttctctag aggtaaaggg tgtatccgat aagtcgggtg tccaaaaaaa
    29401 cttaatatgg tgagtatgta caaacgtact gacgaactag cgaatgtact gtccgaaagg
    29461 catagctcct agaaatggga gagacagtga gcaaaaaatc actcattgtg ctaacttgtg
    29521 gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt acaggcgtag gagaagttag
    29581 ggggcaaaag ctagcatcta agttaaggca tgtggataga tataagggaa caggggaagc
    29641 catcaacatg gagagtgcat actcgatgaa gtggtagtaa gaaatatgtg aatgacaaat
    29701 aacttacttt agtgatggtg aaagtagagt cacgactgat gaaggttctg taatggaact
    29761 ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc cgttcaacca tgggattctc
    29821 gtatgactaa aaaggtcacg agccaattgg agcgtgataa accatgacaa aagaaacaag
    29881 tccaaaagat aggaagttgc ggcatgcaga atattatggc atgacggaaa tcttcgataa
    29941 gatatactcc aaaagtttga agggagataa tttcaagaat ctaatgcaga ttatcatttc
    30001 tgataacaac attgtcctag catatcgtaa tatcaagaga aatcacggta gtgtgacaag
    30061 aggagaagat ggagtaacga ttaaagatat tgaaaatctc aaccgtaatg aattcgtaga
    30121 aattgttagg aagcgattcg cacattataa ccctcggaaa gtaaggcgtg tagaaattcc
    30181 taaatataat ggtaaattaa gaccgttggg tattccatca atatgggata gaatcgcgca
    30241 gcaatgcatc ttacaagtat tagaaccaat atgtgaagca aaattcaata atcacagcta
    30301 tggtttccgc ccaaatagat cagcagaaca cgcaatagca gattgtatgg cccgaatgaa
    30361 tcaaagtcac atggaatatg tggtagatgt agatatcaaa ggtttcttcg acgaagtcaa
    30421 tcatactaaa ctcatgagac aaatctggtc tatgggtata cgagacaaac agcttctctt
    30481 aatcattagg aaaatgttaa gagcacccgt agtacttcca aatgggaagt tgatataccc
    30541 aacaaaagga acaccacaag gcggaatact tagcccatta cttgcgaaca tcaatctcaa
    30601 cgaatttgac tggtggatag caaatcagtg ggaggaaaaa gatgccaaag agctaaaaca
    30661 tcattttagg aaaaatggag cttgggataa aggtaatatc tatcacaagc tcagaaagag
    30721 tacaactcta aaagaaatat acctagtgag atacgcagat gattttaaaa ttttctgtag
    30781 aaattataat gaagcgaaaa aagttttcta tgcaactaaa atgtggttac aggagcgtct
    30841 aaagttacca atatctgaag aaaagtcaaa aatcacaaat ctttcaaaag aagaaagtga
    30901 atttttaggc tttacgatga aattagtgac caaaggtaac aaaaaggttg ttaatacgca
    30961 tataggtaca aaagcacaaa gcatgattaa agtgaaacta aaaaaacaaa tcaaacgaat
    31021 tcaaaaaagt cctaatagca aagatacctt tatctatatt ggagtctata acagtatggt
    31081 tattgggatt cataattact tcaaaattgc gactcattgt agtgtagcaa tgcgaaaaat
    31141 tgcaagagaa ctcagaccaa gttttgaaat acgactacga gaacaaggaa tctcaaaaga
    31201 aggacaatac ggaaagtacg aaggatacaa gccttaccta cggtcaggac aaatacgata
    31261 tataagaggg tttccaatat taccagtagg gtatattcaa cacagacatc cgatgtgtaa
    31321 aaaaagaaat attaataaat acaccgaaga aggtagggaa gccattcata agaagcaaca
    31381 aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat ccagtgctta gtaagagagc
    31441 aacgattgaa tttgcggaca atcgaatttc aaaattcatc gctcaaaagg gaagatgtgc
    31501 agtaacaggt gaggagctta tcttatctga aatgcattgt catcatatta tcccttatca
    31561 tgaaagcaaa agtgatagct atgaaaacct agtgattgtt actgaggagg ttcatcgggt
    31621 aatacacgct acacaaagtg aaacgattga ggaacttcta aaatatctaa aattaaatcc
    31681 aaaacaaaaa gaaaaactca atgagttgag attaaaagtt ggtaacgaag aaatatctta
    31741 ataaatattt gtaggacggg aaaacagaat gtcttttata gacgagggaa cgccgtatgc
    31801 ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac ggagataata tcaaactctt
    31861 acctatcagt atcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    31921 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    31981 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    32041 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    32101 ttggtaaatc aatgacaaac tcagaattag aatccattcc tcaacaaaat caaggagacg
    32161 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    32221 aattaaaatt gtttgatgga ggaaaataat ggaaaaccaa aatgaaagtt taatcaagca
    32281 gtatgttaag cgtcgtgcaa agcgacgctt atttttgtgg ttgtttggta cttctgcagg
    32341 tttgattact attttgatta ctgtgttcgt gacccttttt ctaatacttg cggcgggttc
    32401 gattgataat tctgattcag attcaagtag tggtggcgaa gcttttacag gagaatattc
    32461 ggaaggctta ccgatttata aagaaatcaa aggaagaggc cctttttctg atgagattgc
    32521 gcaatatgct gtaggtgcag cagtgaaata taaattatta ccaagtgtga tcctttcgca
    32581 atacggttat gaatcagcgt ttggtacgtc ggcgtccgca agaaatgatc tcaactattt
    32641 tgggattact tggtttgatg gttgtctgtt tcctaaagga actgcgcgag gaatcggtgg
    32701 tattgaaggt ggctggtaca tgaagtttcc caactcaaaa gcagccttct cctattacgg
    32761 ctttatggta gccacacaat ccaattttaa tgcatgtgta ggaaataaaa gccctggcgc
    32821 aagcttattg atcttaggac gaggtggtta tgccgctgca ggaattactg aagatagtcc
    32881 gtattataca ggttgtatgt ccattatcac ttctaataaa ttaaccgaat atgatgaatt
    32941 cgccatcaaa cattggggtg aaggtggcaa taacaacggc acgatcactg gtgaatggac
    33001 gaatccattc cctggcagca gtttagataa aagttccttc agtggtggtc aattatttgg
    33061 aacaaatcca ggcggtgaat tccgtccgaa tggcttccat gatggattag actttggttc
    33121 agtcgatcat ccaggaagtg aaattcacgc ggtgcatgga ggaaaagtcg tgtacgttgg
    33181 caatcctggt atttcaggtt taggtgcatg tgtgatcgtg attaattacg acggtttgaa
    33241 tatggtatat caagaatttg cgaatagtac aggaaattct cgtgtgaagg ttggcgatca
    33301 agtgaaagtt ggtcaagtaa tcgggatacg agatacagca cacttgcatt taggtttcac
    33361 tcgaatggat tggcgtcaag cacaaggaca cgcattcaca gacgatggta catggattga
    33421 tccattacca tctttgaata gcagcaaaaa atagatcagg agaaaaaata tggaaaataa
    33481 aactttaaaa atcgtcttaa cagctgcaat tagcgttagt ttaatcggca atatcgcgtt
    33541 gttttggcag ctgaaagaag aacaagagac aaataccttt aaaattgaag aagtggctaa
    33601 aaacgcgtct gagaagctca aaaaaaattc ctctgaggca caggcgaaga tcgacaagtt
    33661 aaatcaaacc atcaatgaga aaaaccaaga aatcgatcag ctgaagcaac aagatggcac
    33721 aaaagcaaaa gaggaagtaa ctgaagcaca aagaaagacg gcagaagagt tcggtcaatt
    33781 ggccattgat aaatcattaa gtagaaatga attaacggaa aaactaaaag atgtcgcaac
    33841 ccaagaagtg atcgacaaat taagtcctgc agacgatgat caccaacatg atgattacgg
    33901 aagttactcc tttacattag gtgacgtgca aacttatgct caaacaagca gtgtaaaaga
    33961 ggaacaaaat tttgtcgtat ttgttgatta tacaatcggc aatccgcaat tcaaagatgt
    34021 caaaccacaa aaaataaaag gtggattaac tgtcactgaa aaacaagtag atggtcaatg
    34081 gaaagtcata gactttagtt atttcactcg ttaggaggaa gaaaatgagt aagaaaaaag
    34141 gagaaagaca acgtttcaaa aaacgaaaaa gttatcttca atggctggga atctttatga
    34201 ttctcggctt tttgattctt gctagtccga tttatacgaa tcagaaggta gatataccag
    34261 atgaggtaag cacgaacgcc tttttaaaca cgaaagagct gacaatggtt tcaaaagaaa
    34321 aaggagcgaa ccagcaatta gtttttgaat ttgcgatcga tgatgaagat caaagcttgt
    34381 taaaagaact ggctaatctt aattatcgcg tagaagtaaa gacagctaaa ggggattatc
    34441 aagcaatacg agccaaagtg atcaaggtgt ctgatgatta ttttgcagtg aagctaacgc
    34501 atgtaccaga agaatatatc gccatgcgtt taaccattat tcctgaaaaa attgatccaa
    34561 aagtggatat gcaagaacca caagatctga tttattacgt gcacgaagat aaggttaaag
    34621 atcaagtgaa agatgaagat tatgaacaac acgcgatcaa ttacaaagtg aaaggataca
    34681 agaaagaaca aaaagcagct caaaaacaaa tggaatcttt ccaagcaacc attgatttga
    34741 atgaagaatt agtgaagaag ctaaaagatc aattgcctta tcaagtcgca gatgatcagg
    34801 aaaatactga aaacaagatt aatagctacc agcaagaaat tgaaacatcg aaaacgcaac
    34861 tgaaagatca agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
    34921 aaaagattta gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag
    34981 atactttcgc tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag
    35041 aaatatgtcg agaagtgaat ttatctaaca caacaatatc tgagttgaag aatggtcgaa
    35101 agaatttaga tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat
    35161 attttacagc tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa
    35221 aatgcttatg aatcagctat atcaccttaa ccatttgtta atgagatggg agaaaacatt
    35281 aaccacgatt ctaacgcaaa catcaagcac ataaaacatt taacactcct cctttctggc
    35341 tgaagatttc aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa
    35401 ttcatttttc atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat
    35461 tgtctaaata cacaaataac cttaattttt aaagagcata aggaaacgtg atatcacttt
    35521 ctcacgaaag gtgttaggta ggctgtgcct accacgtttc taagaaagat aaatgatatc
    35581 aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa
    35641 taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc
    35701 cctacacata ataaatttgg aggaaaatat ggacttatta attagaaata ttgaagaaaa
    35761 gtatattaat aaaattgaca aacgttgtga agaacttacg gtcaagactg gcaaaaaatg
    35821 gagtcgaaat caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa
    35881 ttataaaaaa gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa
    35941 tacgaaaact ttagaagaat atattgcaac aaataatcaa ttaattggtt tgttaattga
    36001 ataataaaaa tagtattgtg aatttataaa aggaggaaat caattttggc aaaagcaaca
    36061 agaaaacatg tatatcttta cgatatgcat atcgaagtac ttgatgaaat aatttctact
    36121 catgaagata tatctaatta tagtgaagca gtcagacatt tgtgtatgat gtatgatcca
    36181 aaaatcgaag aaaaaataaa tccaattcaa attaaattga atgcaatggg aaaagaaatt
    36241 tctattttaa ccgaaatggt ttcagagttt gcggatcttc atttgaaaga tacaggaata
    36301 ctaattggta caaattcaac tgtttatcaa gaagcagaag aacgtgtgga aggaaaaatt
    36361 aaatccaatc aaaccaaaaa atattcttct aagttaaaga aaccagtgaa tgaatctgtt
    36421 cataccagag tagatccatt tgatccaacg aagattctaa aaagataaaa gtagaaggag
    36481 aacgtgatga ataaatcgcc tgcagttatt cttacttgtc aatttacctt gcccaataaa
    36541 aaatcttttt caacatacat tgattatatg acaagagaaa aagcactgga agaaatggaa
    36601 cgacgtacgc ccgaagaaga acaggaactg caacgaatca agaatgcttt agaggatttt
    36661 tatattcctg aaggagaaac tttttcggaa agtaagacgg ataaagaatt cagtgaaacg
    36721 tcgaatgaag ctcaatcgtt gatggaacat ggaatcaatt ttgacaagtt agaggaacaa
    36781 gattttacca aatatctttc ttatatgacc cgtcactatg cgttagaaca aaagaaagaa
    36841 ctgacgcaaa gggaacaaaa agaataccaa cggttgaacc aagcaatgaa aaaatatcaa
    36901 acagagccac ataaaaaagc ttcaggaaat cttcctggag ttttttctat ggcttcagac
    36961 gaagtgaaag gaagtgatct gaaggagatc aaacaaatct tccaaagagg acaacaaaaa
    37021 ggctcaatca tttatcaaga tgttatctct cacgacaatt cgtatcttga aaaaatgggc
    37081 ttgtacgatc ccaaaacaga taccttagat gaagaaggtt tgaaagcagc tggaagaaaa
    37141 atgatggaga cattattcga gaaagaacaa ttgaatgaaa caggttactg gatggcaacg
    37201 attcaccgta acactaagca tatccatatt cattttgcag ttgttgaaaa agaaaatacg
    37261 cgtaaaccgt atactgagat tgaaaatggt gtgagttata tcgtaccgaa ggggaagaga
    37321 agacaagcaa cattagacgc aatgaaaacc aattatgtac atgagttaga acgtttctct
    37381 tatgaaaagg atcgaatgat cggtatggag aaacgaaatt tattgacacg aaaaagtgat
    37441 ttgcgtaaca ccttaacaaa agtagtaaaa gaaccagcac gttacgatcg acacgccatg
    37501 aacttattaa atgaagtata taaaacccta cctgaaaaac gttcggaatg gaattatgga
    37561 gatgaaaatc gaaccaagct ttcaccattt acaaggggaa aacttgatga gttaaccaaa
    37621 tatgttttaa caaaagaacc tggatatcaa gaatatatac agatttcaaa agcattgaaa
    37681 gaagaagcca aaagccttta tggggaaagc aaaagagaaa caaagaatgc agaaaaaaac
    37741 gccctctttg atttaaaaaa gcgcacagga aatgcgattt taacagaact gaagagacag
    37801 gatagtcaaa taaaaccatt aatagaaagt gtcaatcaga tgcaatctat ttacaatgaa
    37861 cgatcctttc gaacactcaa acagttgcca acaaatgaat acaagtatag ttatcaagaa
    37921 ttcgtaaagc gtaggaaaca attccaacaa agagttgtca cgcagcgaag cttgcgtcaa
    37981 ttaaatcgaa caattaatgc acatgaagaa aaataccacg caagtaaagc atatgaagag
    38041 caacagcgaa gaatccaaat ggaacaagaa agacaatagg aggaagagaa atgaaacaat
    38101 atttagatca atggaaagtc atagaaggat cactaaaaga agaacgaatc gaacaacttc
    38161 ctgattgttt agaaaaagag catttgtttc agataagcga aatgttgaga aacgaacagt
    38221 ttgatccaaa tcaattttta gttgttgagt acccagctac tggtgtgtat tgttgtaatc
    38281 atgtgaaggg tgaaaaatat tttattattc aagaatacga aggtaagtta gcgccttatt
    38341 atactacttg ggaaataaat gaagagggaa tcaataattt cccatgtgaa agcattgaag
    38401 agtctatttc tctcacggaa tgttaaataa atagagacgt aaaagagtcc tgaacgtgat
    38461 tgtccaggac tctttttcaa tgaattgttt tccgccaatt cactgagtgg tgcgattcaa
    38521 ttttattata tcaaaagaaa gtgagattcc aaagtgaagg gaggaagaag aaatgactca
    38581 gacaacgatt ggtgttgtgg ttgaaaaaag cagggataat ctcgtttttg tgacagaaat
    38641 tcaaacaggt agagcttttg tagtcacaga caaagctgca aaagcgtatc aaaatggcaa
    38701 tattttaacg ttaaatctga ctacgaagat gttcgttgat gcagtagaaa attatccatt
    38761 tgtttagaca aacgatattt tacaaggagg aaacggacat ggcagaagaa aaagaaccac
    38821 tagataatag tcgtttggga aagtccaaaa gaaaattagt acggttgcaa aacgaattaa
    38881 ataatcaatt tcaaaagcta tttgatcacc aaaaattgac aaatgggcaa ccaatgaatg
    38941 acaaacgaaa tggtcgttca tggtttaacc agcaagaaaa aatagaaaat aaaatacaaa
    39001 gtttgagaga agagatcaag caacaagaaa aacaggtaga aaaacttgaa agacaagaag
    39061 agctaaagga aatgggttat aacaagtacg gtggcctgga catgacgatc gaaaatattc
    39121 caagaatcaa agaagaaatc gaacgctttg aaaaaggtga atcgactttt tcagctgcca
    39181 cgatccgaaa ataccaacga aaattggaaa cattagaaca gctgaaagaa aaatcagaga
    39241 aaggaaaaga aaatcttctt cctgaagtac aagcgatcat tgacagcgga cgagtgacgc
    39301 aatggaaaaa gaatccaact atctattttc tgaaaggcta tcgaaaagtc gctttagaac
    39361 tcaatgaaaa cgggggattt gaggaatcta aaaaatacca agccacgtcg gaagaagaaa
    39421 aggcagctgt tcgtgaatta cttgaagaag ctacacctac tgattctgtg aaggagaaag
    39481 aaaccgatca tttctatcga gtagaattca acgaaaatga tccagcaaaa gggttgaaaa
    39541 gttatggtgg ggagatcgta acactagaat tgatcgatca actcaaaaca ctggataacc
    39601 aatacgccgc agaagcaggc tattataaat tctattttga agaagtgaag aatggagaag
    39661 tagtcgatca gatacgaatg gatctagggg atggcgtcaa tagtaatcac cccatctatt
    39721 tagagttagc tagattgtgc gaacaatcac ctgaaaagga acaagaacaa acagtcaaaa
    39781 atgaacctat ttcaaaagta caaaacgaga ttaaagagac aaaaacaaaa caaaagggtt
    39841 atcaaaaacg aacggcagaa gagattaagc aagaagtcaa atcactttcc caacaggcgc
    39901 ttgatgcggt gaaaaagcat actcattcac ctgaagacgt caaagaactt ttgaatttta
    39961 tgtctcgttt tccagaacga tcctttagaa atcaattatt gatcgaggaa caatttccag
    40021 gcgcaacggc ttgtttaggt cgtgccgcgt tgaaaaaaga aggaatctat atcaaaaaag
    40081 gagaacaagg gaacaagatc tttgtcagaa aaacggtcaa aggattttat gagaaaggac
    40141 gtggctttgt tcgagaaaca gaggctacac cagaagataa aaaacgaatc gaaagtggtc
    40201 aaatcgaagt gatcaaaaaa ccttactaca cgattgaaaa agtgtttgat atcacacaaa
    40261 cacaattgaa acctgaagac tatccgaaaa tcttcccgaa ccgcgtcttt gattttcaat
    40321 tggatcaaac agggcagaca gaacttcaag ttgggattca agcggtagcc aaaaatattg
    40381 gaattcaaat tcgagatatg acagaaagtg aagtctatca aagagaatta ggacaagcac
    40441 gtggggcgta cgttcgtaac gaatttactg gcgaagaaga aatcgtcatg aatactcgaa
    40501 atactcctac gcagcattta gccacaagta ttcacgaatt agcccatgct agaatgcaca
    40561 aattttcaga attagatact gcaacaaaag aactacaggc agaaatgacc tcctatattg
    40621 tgtgcaagca ttttgggatg gacacctcgg agaaagctat tccttatatt gcgacatgga
    40681 cgaagaatag tcagacgttg gatgataagg aagctgcgga acgtgggaaa atcatgaatg
    40741 atgtcagtcg tgtcgcaaat gaattcattc aaaccatttc taatgaaatc aatcaataca
    40801 gagaaagaga accagagatc cagccagcag aagtgaagca ggagcataaa aatactgtag
    40861 aaacggcaga acaaaagaat attgtgcccg ttggctcact ttggatcgac aagaaagacg
    40921 ggaaagtcct ggaagaagac acgggccgaa cgttacattt gaaggatgcg gcgtttattg
    40981 aagatccgca gcataatcaa gtgattttaa aaggaaaaag ttatggaaaa gagatccaaa
    41041 cggcgttacc ttcagaagaa tttgcgctag gaaatccatt ccgattttca cttgagacag
    41101 tgtcagaagt ggcaaaacga aaaaatgaaa tctattatga acaacaattg tcacaaaaag
    41161 aacgcgtata aaacaagcaa gaaagactat ctgaaaaatg gatagtcttt tttcttttgg
    41221 gaggtttgaa aatgacagag aaaaaaatga gtctcattga tcgatgcaag caaatggata
    41281 ttgtggattt tgcacgaaac aatggtttag cggtggccaa taaaggacga gactatcgct
    41341 tagaagacca tgattcattt gtttttgatc gaagaaaaca acgtttttat tggaacagtc
    41401 aaaatattag tggcgacatt attgaattag ctaaactttt tttcatagac aaagagatcc
    41461 aagaccctaa gcaacaattt aaagcagcca ctgattttat tctaaagaat gaagacaaaa
    41521 cagaacgagt ggaaaatctt cattttgaga cagaaaagta caaagatcac cccgttgatt
    41581 atcaaccact cacagaaaaa ggaaggaatt atctgaagga agaacgaaaa ctgcccgatt
    41641 ggttgattga ttatgcagaa aaagaaggat tgattgccga actaaagcca aaacatgaac
    41701 ggcaaaattt cttagttcga gacgatcgtt tggatcatgc ggtcgccttt ttatggaaag
    41761 atccacagac aaaagagaca gtaggggcga gttatcaagg aacgttcatt gattatgaac
    41821 gttttggtga gagaggcaca tataagcata tagataaaaa ttcgacagcg aatcatggct
    41881 ttaacctaaa gattggtgat cctaaacagt taaaattttt tgagagtagt attgatttac
    41941 ttagctacgc tgcactgaat cgtgatcagt taaacgatac gtggttagtc tctatggaag
    42001 ggttaaaaca ccatgtgatc agtcattatt ttggtgaggc agtatctgaa ttgagaaaga
    42061 aacaagcctt tcctcaatcc atcgagattt gtgtggataa tgaccgtgca ggacatattt
    42121 tctacgaaaa agaacaatta atgggcgcag ttgatccatt tacgaatcag aaagtacgtt
    42181 gtgaacgagg aatcgctaat gattggcaag tgccaagaga atacaaagtc atttatgaag
    42241 aagtcgcaaa agaagaaaag gtcacaccag aagctattat ggccatacac aagacagaaa
    42301 acaatctgca attaacgaac caactggttt ccgctcacaa agtcaatgcg tcttttggtc
    42361 agcagctttc tgttaacgat tcaatagaag tcattagttt gaaagatatt tgtagagaag
    42421 tcgcaaaaga attaaaagat tgtgaacgct cgaatggtac gtatgatttt gatcgttttt
    42481 atcaaggaaa aggggatatt aattctcaga ttcttttctc ttataaagca gaacaatact
    42541 acaaaggcta taaaaatcat gaacatgaat ttgtacctga agtgaaaaag gattggaacg
    42601 atcaattgaa gcatgagatc catcaacagg aaattagaaa gcaaaaacgt gcgatgttgt
    42661 ttcaacaagg cagacaacaa gaaagggagt gagatcgtca cgatgaaaac tgtcatttta
    42721 gcagaaaaac cgtcacaagc gaaggcctat gccgatagct tttctaaagc cacccgaaaa
    42781 gacgggtatt ttgaaatcca agatcgattg ttttcgggtg aaactgtgat cacgtatggt
    42841 tttggtcatt tagtcgagtt agattcgccc gatatgtacg atgaaaattg gaaacaatgg
    42901 tcattagagc atttgcctat tttccctaca cattatcact atcatgttcc taaagataag
    42961 aaaaagcaat tcaacgtggt gaaacagcaa cttcaatcgg ccgacaccat tattattgcg
    43021 acagatagcg atcgtgaagg ggaactgatc gcttggacga tcattcaaca agcaggcgct
    43081 gatcaaggaa aaacctttaa aagactttgg atcaactcct tagaaaaaga agccatttat
    43141 cagggattcc agcaattacg agacgcagaa gagacctatc ccaaatttga agaagcacaa
    43201 gcacgccaga tcgctgattg gttgatcggc atgaacggca gcccactgta tagtttactc
    43261 ttgcagcaaa agggcatacc aggaagtttt tccttaggca gagtccaaac cccaacgctt
    43321 tacatgattt atcagctgca ggaaaagatt agaaacttcc aaaaagagcc ttattttgaa
    43381 gggaaagccc aagtaaccgc tcaaaatggc atatttgatg cgaagcttga tcccaatgaa
    43441 acccaagcga cgcaagaagc atttgaagcc tatttaaaag aaaaaggtgt tcaagtagga
    43501 aagcagccag gcacgattct tgaagtcgaa accgagaaaa aaagcgcggc aagtccacgt
    43561 cttttttctc tatccagcct tcagtctaaa atgaatcagc tcatgaaagc tagtgccaaa
    43621 gatactttgg aagccatgca agggttgtat gaagggaaat atctaagcta tccacgaaca
    43681 gacacccctt atattacaga aggagagcat gcgtatctgt tggatcattt agatgaatac
    43741 aagcattttt taaaggctga atcattccct actcctattc atacaccgaa tagtcggtat
    43801 gtgaataata agaaagtcca ggaacattac gcgatcattc ctacaaaaac agttatgaca
    43861 gaagcaacgt atgaaaagct gacaccttta caacaagcga tttatgatca agttttaaag
    43921 acgacggtgg ccatgtttgc ggaaaaatat acctatgaag aaactacgat tttgacacaa
    43981 gtccagcagc ttcagctaaa ggcagtcgga aaagtaccag tagatctagg ttggaaaaag
    44041 ctatttggca aagagagcga cagaaaagag aaggaagaag aaccgctact tcctaaagta
    44101 acaaaaggag aaacggtgac ggtagatcta caaatctttg aaaaagaaac caagccacca
    44161 caaccttaca cggaaggtac actaatcacc gccatgaaga cagctggtaa aaccgtggat
    44221 agtgaagaag cacaatccat attgaaagaa gtggagggaa ttggtacaga agcgacacga
    44281 gcaaatatta ttgaaacctt gaagcaaaag gaatatatca aggtagagaa aaataaactt
    44341 gtggtgacga ataaaggaat tttgctttgt caggctgttg aaaaagaacc gctgctaaca
    44401 agtgccgaaa tgaccgccaa atgggaaagt tacttattaa agattggtga gcagaaagga
    44461 acacaagcta ctttcttagc taatatccaa aaatttgtct ctcacttgtt ggaagtcgta
    44521 ccgggacaaa tccagtcaac agattttgga tcaacgttac aagaggtgaa agcagccagt
    44581 gaaaaacagg aagcaacgcg tcatttaggt gtttgtccaa aatgtcggga acaagaagtc
    44641 ttgctttatc aaaatgtggc ggcatgtacg tctgaagcct gtgattttaa attatggaca
    44701 acgattgcca aaaagaaact cacagccaca caactgaaag agatcattca aaacggccga
    44761 acaagccaat cagtcagagg actaaaaggt cagaaaggtt cgtttgaagc aacgatcgtt
    44821 ttaaaagaag actttacaac aggctttgaa ttttctgaaa agaaaaaaac gaactataag
    44881 aaaagaacac gccgcactac aaaataatga aggggttatc ctatgcgaaa tacaacacac
    44941 aaacaacaag aaatcgaaaa tcaaaaagca aaaattcgtt tagaacaaga gaaattacgt
    45001 gcaaataaaa gaagactgca acaattaaag aaccagcagc gcattcaaaa agaaatcgaa
    45061 ttagatcgat agacgttaat cttgttacaa ataaactact atgatatatt tgatgtagtg
    45121 tatttgtaac gaagggaggg gaagtaaatg caagataaac tgaaagaatt gttggctatt
    45181 cgcgatggaa atttatctat gaaggaagcc aggaaatttg gaatcgcagc aaccactgta
    45241 caaagattgg tgaatcaagg aaaattgatc aaagttgatc gtggttatta tgtagaggat
    45301 ggtcatggaa tagatgattt attttggctt cagcaacgat tttccagggg aatcttctct
    45361 catgaaacga ctttagatat ttaccagctt tctacgaata tgccgaaatt cattcactta
    45421 acttttcccc atggctataa cgttgatcgt tcaattacaa aagagcagca gttacaattt
    45481 catttcgtga agaaagaagt ttatgaacta ggaaaagtag agggaacttc ctttcaagga
    45541 aatcctatta ctatgtatga taaagagcgt acactttgtg atatttggaa tccgagatat
    45601 gaatttgaat atgaaatgaa attagaagca ttaaaagagt acatggaaga cccattgaga
    45661 gatccaagta aattaagaga ttacatgaca aaactacatg tagaaaaaga aatgaaatac
    45721 cacatgcaaa gcttatattg atggaggaaa aagatgacac cgacacaatt tgcagcaaaa
    45781 acaagaaatt tagcaaagag tacaggatta aatgcacagt tagtgcaacg gcattttatg
    45841 atggatcgtc taattgaaca aatttctgaa tcaaagtaca aaaatgactt cattttgaaa
    45901 ggcggctttt tattaggatc aaaatatgga atcgatagaa ggacaacaat tgatatcgat
    45961 acgacattta gaaatagcaa attaactgaa gaaaaattga agagcatttt taatgattta
    46021 actagaaaac ccacaaaaga aggcattgtt ttctctattc aaggaatgaa ggaaacaaga
    46081 gaagccgatt tttatcctgg atttcaaatt aaattggtgg ctaatttaga gaaagtacgt
    46141 gtaccgttta aaatggacgt gacaacagga gattctattt atcctgaagt agatacacac
    46201 tatcaccaat taatgtttga agataaaaaa gtggagattc cagcttatcc aacagaacaa
    46261 attattgcag aaaagctaag tgctacgttc agctttggca cagataattc gagagcaaaa
    46321 gattactacg acttatttac cattccgaaa ctagaaaata tcgatacgaa agagttatac
    46381 aaaagtgttc gaaatacatt tacaaaacgt ggggatacaa aaccgttgtc cttttattat
    46441 gaaagagaaa tggataagat cagaggaaat gactatttgg aaaagcagtg ggaaaaatat
    46501 cgctcagtta acacgtttgc gaattccatt tcatttacag atacagtcaa cgaaattgat
    46561 cacctaatga aaagtgtgat caaaattgaa aatcaagaaa gaaaacggca gtttaaaaga
    46621 tcacaacaaa tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag
    46681 gacgcttaac caaagatcca gatttacgtt ataccgcgag tggtactgca gtagcgactt
    46741 ttaccctagc tgtcaaccga aatttcacga atcaaaacgg caatcgagaa gctgatttta
    46801 ttaactgtgt gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaagggca
    46861 gtttgatcgg cgttgttgga cggattcaaa cccgaaccta cgataaccaa caaggacaac
    46921 gtgtgtatgt gacagaagtt gtggccgata atttccaact attagaaagt aaagcagcga
    46981 cagaaagccg tgcgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac
    47041 aacgggatgt agctacgcct aacaacaatg gattgaacgc ttcacaaaat ccatttggcg
    47101 gacaatcaat cgatattagt gatgatgatt taccattctg attgtaaaag atcaataaag
    47161 ttgttataat ggatacgaaa agaggagttg cgctaacaac tcccctaaca gcaccgttta
    47221 agacggtgac cagcttaata ttatttttga aaaataatcg tcgaccggct aaagctgacg
    47281 gttatttttt ttgattgttg tttacaactg ctaagattcc aaaaatgatt gttgcgatca
    47341 aagaaccgaa actaatcatt aatcctagcg ccgtttcaac aggcaaaagg cttctccttt
    47401 ctgtaagatt ttggaagtac gcacatacgc accacctcac tttcaatcgg atagccaccg
    47461 tcacaaactt tactgtcttg atcattatag catatctaaa atcgaataaa aattctcaat
    47521 tttagctgtt tttgctataa ttttagcgag gtgatcgtga tgaacaaata tgttggtttg
    47581 cttgataaaa tccgagtaat aaaaacacaa ccattattgg ttcgctttac tttacaaaca
    47641 attcatgaat cgattaattg tgtagttgca gatattgaaa ttatcgataa attgttaatt
    47701 atggatgatg gaaaatacaa tattgccgtc acaggtcatt tcaataaacg gaatcaatta
    47761 gtcattgaat cgatgcaaat tagaaacccc gatcatttta cgcgatcgat gggcatataa
    47821 aagaagagct gcagtcctaa atgaggattg cagctctttt tgttttacat agaaacagga
    47881 aaagaccttt ttaggaagac acaagtgcct tctcaattga aaaacctttc tctcactcca
    47941 accgccaccg ccgttcgaga agaaaggaag tgaacgttgc acgttcacta agatatggtg
    48001 tcttgcaaag caagacaaaa tagaaaaaac ttcctctcac caaaaggttc gttcctgttc
    48061 gctatctctt tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga
    48121 caaggacaag ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca
    48181 ttctggccaa caaatcaata aagaaagaac taacgaaagg aaataaaacc tttcctaaga
    48241 ggaggcagaa tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt
    48301 attcccaaac tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg
    48361 tatcaaaagc tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta
    48421 tggcgattat gttttgattg atgagaaagt aaagaaacgg tgatgaaaac acaacacaaa
    48481 ttattgactg taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa
    48541 gtagcagaag ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat
    48601 gcaccgacta tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa
    48661 gaaaacatgg aaacttatga aatcgaatat tggttaacca aattagagaa ctaaaaagga
    48721 gaaaaaatat ggctcaaaat tactatgacg aatttgtcaa attacctttg gataaaatgg
    48781 cacaaaaaat ggaggatatg acgttcctat accatgaaac acgtgtaccc aaaaaacatt
    48841 acaaggaaaa attgtcagtc gcagtggaag aaatgattga atcaggtgtc gaaatgaatc
    48901 tcattgcgac gtactatcga acactggaag aactgaaaaa acaaaatgga aaatggtttt
    48961 tccaagcctt actatgcttg gaagtaggtg tcaaaccaag cactatcaaa ccttccgaat
    49021 atcaagcctt agaactgact tatgcaaaat tcgcacagat taagaaagct aaagcaatgg
    49081 ctagcgactg gctagatact tttgaaaata tcgaaaaaaa tggtgcattt tatacgattg
    49141 aatcggagga gaaagataat gaataaggtg gtttatcagc taacaacaga agatgtttcg
    49201 caatttttaa caaacaaaga acacttgaat gaagtaatca gacggattca aaaagacttt
    49261 agttgtatgg aagaaatgaa gtactttatt caaagagtgg aagaacagtt gtatatggaa
    49321 agtgaaacga gtcaaacgaa ggttacagtc agccataatc gtttagacgt gtataaacgt
    49381 cgtcctgaca cattaaaaaa aggagcggag cttctacgac tagaaacaga aaaatatatc
    49441 atgaatgttt acctagaaag gagtgagctg gtcgaaaact ttgtcagcta cacgatttgc
    49501 aggaaacact attactcaga atcagaatat tattcaattt ttgagagcat ggaattaatg
    49561 gaagaagaaa tggaagagct aaacagcaaa gatgcgacgg tagacgatta ttaccttgta
    49621 tttactagat ttttgacgtg tgggacggaa tctatcgtgt taatgggaat ggattatgaa
    49681 ctcactgtac ccgaagcatt tttgagggaa tatgtagaag aagtagataa cccaggcgac
    49741 gactggtaca cgactttaac tgatttttta gacacctaca cttacgacga tacagaagaa
    49801 atccaaaatg aacttgaccg taaacgaatg ccatatcaac gatttgaaag gaaaaaaggt
    49861 caataaccat ttataccgaa aagtgaggat gtgtgaaata agaacaaaaa accttgaaat
    49921 taagggaatg gttcttattt cgcaccaatc gatccaaccg cccccgccgc ttaggtggtg
    49981 cgaaataagc gggcaaagcc cgataaccta agagaaagaa ggagatccat atgaataaaa
    50041 tcattcctct attgttcgta ctgtttgttg gcgagatact tatgcgtcat ggtgcagctc
    50101 tacatttaaa cttcgtagta ttcttaggaa tactggtatt ttgtttgatc ttttggcgct
    50161 ttgtgttaac ccttgtgagt ctaatattca agttgattgc agccatattt ttaatagggt
    50221 tatttcgatg aaaggagaga gtactaatgc caattgcaat aggaaataaa cggttacccg
    50281 tgacgttgga tgaaaagaga caaaaagaat tgcagcaact aaagcagaag tacggcaaaa
    50341 gtgaatccag gattatgtgt attgcgttag atttattgat tgcccaagaa aaagcaggat
    50401 ttgaggtacc agcactcaaa aagtgacgtc actttttatc ctaaaaacta aaagtgatag
    50461 ca
//
LOCUS       DAJSBM010000015        47952 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJSBM010000015 DAJSBM010000000
VERSION     DAJSBM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.015"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(38..1189)
                     /locus_tag="ONT67_001023"
     CDS             complement(38..1189)
                     /locus_tag="ONT67_001023"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR2354779.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="ONT67_001024"
     CDS             complement(1202..1639)
                     /locus_tag="ONT67_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2354780.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="ONT67_001025"
     CDS             complement(1654..3420)
                     /locus_tag="ONT67_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR2354781.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="ONT67_001026"
     CDS             3684..4361
                     /locus_tag="ONT67_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354782.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="ONT67_001027"
     CDS             4364..5491
                     /locus_tag="ONT67_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354783.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="ONT67_001028"
     CDS             complement(5691..6131)
                     /locus_tag="ONT67_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2354784.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="ONT67_001029"
     CDS             complement(6142..8175)
                     /locus_tag="ONT67_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2354785.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="ONT67_001030"
     CDS             complement(8177..9880)
                     /locus_tag="ONT67_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2354786.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT67_001031"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="ONT67_001031"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCR2354787.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="ONT67_001032"
     CDS             complement(10963..11988)
                     /locus_tag="ONT67_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2354788.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="ONT67_001033"
     CDS             complement(12197..13066)
                     /locus_tag="ONT67_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCR2354789.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="ONT67_001034"
     CDS             complement(13525..15417)
                     /locus_tag="ONT67_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354790.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="ONT67_001035"
     CDS             complement(15414..17141)
                     /locus_tag="ONT67_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354791.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="ONT67_001036"
     CDS             complement(17157..17807)
                     /locus_tag="ONT67_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2354792.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="ONT67_001037"
     CDS             18011..18307
                     /locus_tag="ONT67_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2354793.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="ONT67_001038"
     CDS             18479..19099
                     /locus_tag="ONT67_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR2354794.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="ONT67_001039"
     CDS             complement(19191..20357)
                     /locus_tag="ONT67_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2354795.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="ONT67_001040"
     CDS             complement(20369..20722)
                     /locus_tag="ONT67_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2354796.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="ONT67_001041"
     CDS             20850..21872
                     /locus_tag="ONT67_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCR2354797.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="ONT67_001042"
     CDS             21891..22052
                     /locus_tag="ONT67_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354798.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="ONT67_001043"
     CDS             complement(22311..22745)
                     /locus_tag="ONT67_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354799.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="ONT67_001044"
     CDS             complement(22855..24714)
                     /locus_tag="ONT67_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2354800.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="ONT67_001045"
     CDS             complement(24832..25992)
                     /locus_tag="ONT67_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HCR2354801.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT67_001046"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="ONT67_001046"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR2354802.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="ONT67_001047"
     CDS             complement(27739..28437)
                     /locus_tag="ONT67_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2354803.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="ONT67_001048"
     CDS             complement(28394..30055)
                     /locus_tag="ONT67_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCR2354804.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="ONT67_001049"
     CDS             30273..31673
                     /locus_tag="ONT67_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR2354805.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="ONT67_001050"
     CDS             complement(31925..33004)
                     /locus_tag="ONT67_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2354806.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="ONT67_001051"
     CDS             complement(33090..34049)
                     /locus_tag="ONT67_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2354807.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="ONT67_001052"
     CDS             complement(34061..35572)
                     /locus_tag="ONT67_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2354808.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="ONT67_001053"
     CDS             complement(35594..36157)
                     /locus_tag="ONT67_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCR2354809.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="ONT67_001054"
     CDS             complement(36163..37026)
                     /locus_tag="ONT67_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2354810.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="ONT67_001055"
     CDS             complement(37040..37936)
                     /locus_tag="ONT67_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2354811.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="ONT67_001056"
     CDS             complement(38111..39400)
                     /locus_tag="ONT67_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2354812.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="ONT67_001057"
     CDS             complement(39372..40889)
                     /locus_tag="ONT67_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCR2354813.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="ONT67_001058"
     CDS             41087..41977
                     /locus_tag="ONT67_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR2354814.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT67_001059"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="ONT67_001059"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR2354815.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="ONT67_001060"
     CDS             complement(42886..44358)
                     /locus_tag="ONT67_001060"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR2354816.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="ONT67_001061"
     CDS             complement(44995..45357)
                     /locus_tag="ONT67_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2354817.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="ONT67_001062"
     CDS             45580..46332
                     /locus_tag="ONT67_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2354818.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="ONT67_001063"
     CDS             complement(46472..47395)
                     /locus_tag="ONT67_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2354819.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="ONT67_001064"
     CDS             complement(47410..47682)
                     /locus_tag="ONT67_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354820.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DAJSBM010000016        43693 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJSBM010000016 DAJSBM010000000
VERSION     DAJSBM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.016"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(54..398)
                     /locus_tag="ONT67_001065"
     CDS             complement(54..398)
                     /locus_tag="ONT67_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCR2354821.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(391..825)
                     /locus_tag="ONT67_001066"
     CDS             complement(391..825)
                     /locus_tag="ONT67_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCR2354822.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            963..1700
                     /locus_tag="ONT67_001067"
     CDS             963..1700
                     /locus_tag="ONT67_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2354823.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1693..2904
                     /locus_tag="ONT67_001068"
     CDS             1693..2904
                     /locus_tag="ONT67_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354824.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3108..3890
                     /locus_tag="ONT67_001069"
     CDS             3108..3890
                     /locus_tag="ONT67_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCR2354825.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            3960..4622
                     /gene="trmB"
                     /locus_tag="ONT67_001070"
     CDS             3960..4622
                     /gene="trmB"
                     /locus_tag="ONT67_001070"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR2354826.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4720..6474)
                     /gene="ade"
                     /locus_tag="ONT67_001071"
     CDS             complement(4720..6474)
                     /gene="ade"
                     /locus_tag="ONT67_001071"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR2354827.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6606..7535
                     /gene="tadA"
                     /locus_tag="ONT67_001072"
     CDS             6606..7535
                     /gene="tadA"
                     /locus_tag="ONT67_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCR2354828.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7492..8541
                     /locus_tag="ONT67_001073"
     CDS             7492..8541
                     /locus_tag="ONT67_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCR2354829.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8538..8816
                     /locus_tag="ONT67_001074"
     CDS             8538..8816
                     /locus_tag="ONT67_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR2354830.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8791..9261
                     /locus_tag="ONT67_001075"
     CDS             8791..9261
                     /locus_tag="ONT67_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCR2354831.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9278..9580
                     /locus_tag="ONT67_001076"
     CDS             9278..9580
                     /locus_tag="ONT67_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354832.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9597..9968
                     /locus_tag="ONT67_001077"
     CDS             9597..9968
                     /locus_tag="ONT67_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCR2354833.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9940..10299
                     /locus_tag="ONT67_001078"
     CDS             9940..10299
                     /locus_tag="ONT67_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354834.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10471..11478
                     /locus_tag="ONT67_001079"
     CDS             10471..11478
                     /locus_tag="ONT67_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2354835.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11503..12687
                     /locus_tag="ONT67_001080"
     CDS             11503..12687
                     /locus_tag="ONT67_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR2354836.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13124..14707)
                     /locus_tag="ONT67_001081"
     CDS             complement(13124..14707)
                     /locus_tag="ONT67_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2354837.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15285..15428)
                     /locus_tag="ONT67_001082"
     CDS             complement(15285..15428)
                     /locus_tag="ONT67_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR2354838.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15650..16129)
                     /locus_tag="ONT67_001083"
     CDS             complement(15650..16129)
                     /locus_tag="ONT67_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2354839.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16202..16486)
                     /locus_tag="ONT67_001084"
     CDS             complement(16202..16486)
                     /locus_tag="ONT67_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354840.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16564..16758)
                     /gene="ssrS"
                     /locus_tag="ONT67_001085"
     ncRNA           complement(16564..16758)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ONT67_001085"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16830..18116)
                     /locus_tag="ONT67_001086"
     CDS             complement(16830..18116)
                     /locus_tag="ONT67_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCR2354841.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18254..18925
                     /locus_tag="ONT67_001087"
     CDS             18254..18925
                     /locus_tag="ONT67_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCR2354842.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18891..19379
                     /locus_tag="ONT67_001088"
     CDS             18891..19379
                     /locus_tag="ONT67_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCR2354843.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19392..20339
                     /gene="prmA"
                     /locus_tag="ONT67_001089"
     CDS             19392..20339
                     /gene="prmA"
                     /locus_tag="ONT67_001089"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR2354844.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20340..21104
                     /locus_tag="ONT67_001090"
     CDS             20340..21104
                     /locus_tag="ONT67_001090"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR2354845.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21337..23550
                     /locus_tag="ONT67_001091"
     CDS             21337..23550
                     /locus_tag="ONT67_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR2354846.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23561..24007
                     /locus_tag="ONT67_001092"
     CDS             23561..24007
                     /locus_tag="ONT67_001092"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR2354847.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24178..25437
                     /locus_tag="ONT67_001093"
     CDS             24178..25437
                     /locus_tag="ONT67_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCR2354848.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25526..26473)
                     /locus_tag="ONT67_001094"
     CDS             complement(25526..26473)
                     /locus_tag="ONT67_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2354849.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26867..28159
                     /locus_tag="ONT67_001095"
     CDS             26867..28159
                     /locus_tag="ONT67_001095"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR2354850.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28174..29943
                     /gene="aspS"
                     /locus_tag="ONT67_001096"
     CDS             28174..29943
                     /gene="aspS"
                     /locus_tag="ONT67_001096"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR2354851.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30052..30927
                     /locus_tag="ONT67_001097"
     CDS             30052..30927
                     /locus_tag="ONT67_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2354852.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31193..33076
                     /locus_tag="ONT67_001098"
     CDS             31193..33076
                     /locus_tag="ONT67_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2354853.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33151..33435)
                     /locus_tag="ONT67_001099"
     CDS             complement(33151..33435)
                     /locus_tag="ONT67_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2354854.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33425..34159)
                     /locus_tag="ONT67_001100"
     CDS             complement(33425..34159)
                     /locus_tag="ONT67_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCR2354855.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34288..34911
                     /locus_tag="ONT67_001101"
     CDS             34288..34911
                     /locus_tag="ONT67_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCR2354856.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35103..36224
                     /locus_tag="ONT67_001102"
     CDS             35103..36224
                     /locus_tag="ONT67_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCR2354857.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36231..39359
                     /locus_tag="ONT67_001103"
     CDS             36231..39359
                     /locus_tag="ONT67_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCR2354858.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39378..42572
                     /locus_tag="ONT67_001104"
     CDS             39378..42572
                     /locus_tag="ONT67_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCR2354859.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42718..43425)
                     /locus_tag="ONT67_001105"
     CDS             complement(42718..43425)
                     /locus_tag="ONT67_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2354860.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14093 a         7755 c         9228 g        12617 t
ORIGIN      
        1 tacgcaaact tttctcactt atatagttaa atctgttgag aaaataatca ttattatatt
       61 tgttcaggat actctggcag atcagctttt agtttttcta cttgttcatt gatttgtgtg
      121 atacgtggtt cggcttggaa tttaaaatct tcgatatctt ttttgattgc ttcttggaaa
      181 gcaggaatca cagattcagc tgtttctttt gtttcgatca atgcgctttt aaaatgattc
      241 aggctgttat ttaaatcatt tgtcagagtt gttgcttctt ccaattcttc gatcagtttc
      301 tttcttgttt cattcccgct cctgggcgcc aataacagac ctccgacagt gccagcagca
      361 gctccaaata acagtccttt gaaaaagttt ttaatcatcg caggacacct gctttacgat
      421 tgtttcttga atagctttta gttcttccga tccataacta tcttgatgat tgacaaagtg
      481 aatagaaaaa tcatcctctt tgctgtagcg agggactaaa tggatatgtg aatgaaaaac
      541 agattgatag gctagttcac ggttgttatt caagatatta agacctttca tttctggaaa
      601 agctttttcc aatgcttgag caatcttagg cacacgcgaa aaaacctctc ctgcaagttc
      661 gggatcatat tcaaaaatat ctgctacatg acgtttaggc acaactaatg tgtgtccttt
      721 cgttgcttga gagatgtcta aaaaagcata gaccttgtcg tcttcatata ttttatagct
      781 tgggatttct tgattgatga ttttgcaaaa aatacagttt tccatataaa acatcctttc
      841 tataaagaat aaatacttca gcttaacttt accataagtt aaagctattg taaggaaaaa
      901 aacaaaatac cgtcattttc ttcgtggtat aataaataaa aataaatggc ggaggaaaaa
      961 gaatgagttt aattatcgat catgtcacag gggggtacgg acatctccct gtgttaaaag
     1021 atatcagttt cgatgtacag cctggagaga tagtagggtt gattggactg aacggagcag
     1081 ggaaaagtac gaccatcaag cacattatcg gtctgctgga agcttcaaaa ggaacgatag
     1141 aaatcaatca tctatcctta aagaaaaata cggaagaata tcgcaaacaa attgggtata
     1201 ttccagagac tccttcttta tatgaagaat tgacattgag agaacatatc gaagtcactg
     1261 cgatggccta cgatatacct atggatatag caatgaaaag agcagaaaag ttgttggaga
     1321 cttttcggtt atctaatcgt cttgactggt tcccagctaa tttttcaaag ggaatgaaac
     1381 agaaagtgat gatcttatgc gcctttttga tcgagccttc tttatatatc atcgacgaac
     1441 catttttagg cttggatcca ctagctatca atgcattgct tgaattgatg gtagaaatga
     1501 aaaaagaagg agccggtatt ttgatgtcta cgcatatttt agctactgca gaaaaatatt
     1561 gtgatcgttt tgtggtctta cataacggag agatacgagc agacggttct cttgagatgt
     1621 tgagaaaaga atttcagctg ccggattctt ctttggacga aatctatatt gctttaacaa
     1681 aggagcaggc acatgagtag tttttttcaa aaacgtttag caagacatca aaagaaaatg
     1741 atgaaatata tgcgttatgt attgaacgat cattttgttt tagtttgtct tttcttgatt
     1801 ggcggtgtgg gattatatta ttctaattgg ttgaaaaccc tgcagccacc gtttgcaatt
     1861 ggagggctca taatctctgt attttggatg ctctgtttat tcgtgggtaa gatggctact
     1921 tttgcagagg cagcagatat tgttttttta cttcctaaag aaaaagaaat gcaaacctat
     1981 ttggaacgag cttttcgcta ttcttgcatt tttccgttga tctttttgat attggcatgc
     2041 ggattggcca tgccattagt ggtggttagt acggagcaat ccttttcgat gtttttcgtc
     2101 tatctgatca tattatggag tttgaaattc agtcatctgc aaatcaagag agcagaattg
     2161 tttcgactag atcaacaagt tattcgttat tggaaattag gctggttttt ttcttctctg
     2221 atctttttgt ttctatcatt ttattggcta atatgggcag gaatagcagg tgcattagta
     2281 caggcaagtc tatattactg gtttttgtgg aagcaacaag aaaaaggatt ggattgggag
     2341 aaaatggtgc atgtagaaga acataggttg catcagatct atcaatttat caatttattc
     2401 acagatgttc ctgaaattac ggcaaagatc aaaagaagac gctatttgga tcctatattg
     2461 actcatatta aaagagaatc aaaaaacact tatctttact tgtttgcaag aagattggca
     2521 agaggttctg attttagtgg tctttatttc cgattagtaa ttataggtgg gttattgatt
     2581 gccggagtag tcgactttta ttttagtctg attatcggtt tcttattttt atatttgatc
     2641 ggtttccaac tgttaccttt agcgaaccag ttccgctata tggttctcgt tcagctttat
     2701 ccaatccata agaatcagaa gaagcaggcg attcagtatc tactcacatg ggtattaatt
     2761 atcgcgtcgg ttatttttgg tttgattgct gcagtcgtat tagatgggca gcagcgatgg
     2821 gttccgctag cggcttattt gattgttact atgctctttg ttaaactata tgtaccaact
     2881 cgtttgaaga agatgtcaga ctgaaaacaa agctgtgtag ggtaaaaatg agaatgaaaa
     2941 atagagcgtt gacagtaaga atgctgttcg ttctattttt tttgagaaaa gctggttttt
     3001 tgaatggcaa ttaaatgaaa aagaacgtat atgtgcttga cgtgtagtgt tcaggcgact
     3061 agaataataa gtactgcaaa acaaagagta agaggacggg agaatgcatg atggagtttc
     3121 agttagacca agagtggcgg ttacgtccca tcaaaggcga cacaggaaaa acatacatag
     3181 gcatgcgtga tgaggacaaa gtctttatta aacgaaacac gactccgatg ttggctgctc
     3241 tttcaaggga aggaattaca ccaaaacttg tttggacaaa acgaacgggg aatggcgata
     3301 ctttgacagc acaagagtgg ctggaaggtc gcttgctgac tcctaatgag attggtcaaa
     3361 gaaacgacgt gattgatgtt ttgtaccatt tacatcattc aaattctctt aaaaccatgc
     3421 tgacccgtat cggtggtcgt gtcatgacgc cggagaagat gctgcaaaaa tatgaaaaag
     3481 aattaccgga agaactccag aaaaatcaat ttctgcaaat tgtccatcgt tttcttcgtg
     3541 agcagatccc gtcaatagat aaggaaaact tatcagtagt acacggagat gtaaaccatc
     3601 gtaattggtt agtatgtaaa aattatttat atctagtcga ttgggattca atcatgtttg
     3661 cagaccctgc ggtggatatt gcaacgattt taggaaatta tgtgccactg tctagctgga
     3721 atcagtggct gatcagttat ggcatacgcc cgactgacga aatgcttgaa aaatttcatt
     3781 ggtatgcggc catgaatttg ctccaagaaa tcaccagata ttgcaaacgt gaagatcatc
     3841 gacggatgaa cgaagaaatt ttacaattaa aacgaatttt cagtggctga ggtttggccc
     3901 aaactctgcg gagatttggc cagacctttt taattagagg aaaggaatgg agataaataa
     3961 tgcgtgtccg aaatcgtccg ggagcagcgg aaatgttagc ggctcacccg aattttgtaa
     4021 ttagtgatcc aacattgtgg aagggaaaat ggaatgaatt gtttgaaaac gatcatccaa
     4081 tccacattga gattggtatg gggaaaggcc agttcatcac tggaatggca aaagcccatc
     4141 ctgaaatcaa ttatattgga gtagaaatgc aggtcagtgt tgtttcgatt gcactagaca
     4201 agttgatcga acaaccgctt cctaacctga aacttctaca tgtggatgga tcagcgttga
     4261 cagagtattt cgctgacagc gaagtagatc aaatctatct gaatttttcc gatccatggc
     4321 caaaaaaacg tcatgaaaaa cggcgattga catataagac ttttcttgct gtagatgaac
     4381 agattcttcg tccaaatgga gaaatccatt tcaaaacaga taatcaagga ttgtttgagt
     4441 attctctggc aagtttttct caatatggca tgatcctcaa acaggtatgg ttagatttac
     4501 atcaaagtca atttgaaggc aatattatga cggagtatga agaaaaattt tcttcaaaag
     4561 gtcaacgtat ttatcgagta gaagctcaat ttcaagataa gaccaaaaag aaaagtctgt
     4621 gaaactaact ttctgctttc ataaaaacat taatatccat aaataaatct cagaaaatga
     4681 acattacaga tgtaatgtat tttctgggat ttatttgatt taaaatgtat ttatttcatc
     4741 ggtttttcca caaggtggaa tcaattggaa gcgagaaaat gaaaataatc ctttgtcggt
     4801 gatcttcaat tcgggtatga cagataatgt taagaaagaa agtgtcaaaa aaggatcaaa
     4861 ggttttagct gctcctagtt gatgtgcttt agttattaat tcattcaact gttcatagac
     4921 gatcgaaaaa ggttcctgtg tcatgattcc gcctatagga agagaaacac aagctatttc
     4981 ctgctgtttg ttgacaacgg tcatcccgcc acctttttga atcaaaagat tagcagcata
     5041 cagcatatcc tcgtcgtttg tcccaacgac aactagatta tgcgagtcat gggcaatcgt
     5101 tgtcgctatc gctccttgtt tcagctgaaa tcctttcact atgcctaatc caatgtttcc
     5161 cgtttgatgg tgacgctcga tcaccgcaat ctttaattgg tctttttcga ttgaaggaat
     5221 gaatcgttcg ccttgtcggt ccactttttc taccaaatct ttagtcacta gactattcgg
     5281 gataatttcg atcacatggg cttgtgaaga agaaagaagc agctctaacg aatgtggatt
     5341 taatggttga gcgtttagtt ttggcagaca acacgtatat gaatttgaat gatcagcaaa
     5401 taaatggtct tggagttgcc cagattgaac aactaatgtt cccttataga aaacttcatg
     5461 aattggcagc ttggtaagat cgtctgttaa gaagaaatca gcttgatacc caggggcaat
     5521 cgcacctagt gttttcaaat caaaacattc tgctgcattc aatgttgcca tctgaatggc
     5581 ttgtaatgga tcgattccaa gtgcaatagc ttgacggacg atatgatcga ttgatccttc
     5641 ttttactaaa tcattgatca gtttatcatc ggtgacaaag aagcatcttc ttgaattttc
     5701 aggtgtgatc gccggtagta gcgatgccag atcttttgca acggtccctt ctcgaaccat
     5761 taaatacata ccaagatcca aacgttcttt agcttctttt gcagttgtag cttcatgatc
     5821 cgtccgaata cctgctgcag gataaacatt taactcttcc atgccgatac cagctgcatg
     5881 cccgtcaatt tttttcttat gctggtgcat caattggatc ttttctagga tgtctgtttc
     5941 attatttgct actgcttgat agttcatgac ttcagctaag cctaaaactt cgggtttttg
     6001 taaaaaaggg gctaattttt ttgcatttaa atttgcacca ttggtctcaa aaggggttac
     6061 aggtacacaa gaaggtagca taaaaaagat attcatgagt gtttgtcttg catcttctat
     6121 cataaactca agacctgtac tcccagcaat gtttcctatt tcatggggat ctgtcactac
     6181 agtagtaact ccatgaagca aggatacttt tgaaaattcg cttggtgata aaagtgagct
     6241 ttcgatatgc acatggccat cgatgaatcc tggcactaga tattgcccat tagcattatg
     6301 tacgcttttt ccttcgtatt caccgattcc tacgatcatt ccttcgcaga ttgctacatc
     6361 tttttccatg atttctttcg taaagacgtt gacgatctta gcatgcttga tcactaggtc
     6421 agcaggcttt tttttacttg ctacttcgat atatttcctt aatgttgttt ccaatggagt
     6481 gcctcccaag ataaattttt ataagtataa cataaattcg gcttttgcca atattttttt
     6541 gtaaattcat tttatttgaa acaaaaagat caaaaagacg tatgtttttt gtggagggga
     6601 gaataatgga aataaaacga ttatcggatc aaatgataag tgaaagtttt gccaaacatg
     6661 cccaagacgt atatatattg ccagcttcag aagtatttta catctatttt cgctatggaa
     6721 aaaaacgaac attctggaag aaagtctctt cggcagatgg acagcaactg atctcaagat
     6781 ttaagtattt gggacagatg gatatcggag aaaaaagaaa agctcagtta ggtgcattta
     6841 cttattcttt agaaaaacag gaagttcgtc tgcggttgtc ttctgtagga gattaccttc
     6901 aacgagagag tttagtgatt cggctattat acggtacttt tgaaacaccc catcgttgct
     6961 ttagttctcg agattttgca actattttac agaaaaccgt cagacgtgga ttataccttt
     7021 tcagcgggac agtcggatca ggcaagactt ctctaatgta tcggttggct ttggaagaaa
     7081 aattgcaagt gattactatc gaagatcctg tagagataga agaaccgttt tttctgcagc
     7141 tacagatcaa tccgaaaata gcacagactt atgatacact tttgaaactc gcattacggc
     7201 atcgtccaga cttgctaatc attggcgaga taagagatga atcaactgca catgctgcgg
     7261 taagagcagc actaacaggg caccgggttt ttgcaaccgt acatgcacgc aatcttgaag
     7321 gtacattggt cagaatccat gaactgacaa agaaaaaaga agagctatct gaatgtttag
     7381 ctggtgtcat ttatcaagaa cttttggaag atgccaatca ccatccttct gttttatgga
     7441 gttatcaatt tatggaggaa gaatcagtaa aaaaatcatg ggaggaaagt tatgaagaag
     7501 ctcaggaaaa tatttggcag tcagaactat tctaaaggat taacaagaag ccagcaaaac
     7561 cagtttgcac aaaccatggg agaactgctt ttatctggtt ttagtctgca agaatccctt
     7621 tatttattac tcacgatccg ttcgtttcca cctgttattt tggcaaatat ccaacatcag
     7681 ctaaaggaag ggcaagcgtt tagtgaggta ctttcgcaaa taggatatgg agcagagcaa
     7741 ctggtacaga tcgagttagc tgaacgtcat gggaatctgc cgacaacttt gttggaaatt
     7801 gccaaacagt ttcggcttgt tgaaggattt cgcaatgatt tgaaaaagct gcttgcatat
     7861 ccgatgctat tgctcacctt tttgctaggt atcttgttta gtatgcggtt gttcgttttg
     7921 cctcagctta ttggttcagg aatggtcatg caagagcatt ggggtattca gctgatacaa
     7981 gtctttcatt ggtatgttct actattcatc ctagcggtta ctctattggc tttactagta
     8041 aggtatcgtc tgatgaagtt gtctcctgtt gatcagagtg aatggataag cagacaatta
     8101 ttgattggac cgatatattc cttgtatcaa tcggcttatt tgtcactaga gattggaaag
     8161 ctttttcatg aaggtttcga attaaggcaa atcatagtct gcttaaagga aacgaaacaa
     8221 gaaagtctga ttcagcatct tgcctctaag ttgattcaag gattggaggc aggtatgtca
     8281 ttggctgagc agttccagaa ttatcgtttt ctatcagaag aattcagtaa aattgtttta
     8341 cagggagagg caaagggaag tttaggtaaa gaactgattt tttacagcga atggacaaga
     8401 aaacagcttt tcaaacgtct tcatcactgg atgcatttta ttcagccaat catttttttg
     8461 ggcgtagcgg ctttgatttt attagtttat gccgctgttt tattacctgt ttacggaagc
     8521 atagaggagg ttttgccatg aaaaagagaa aaatatctta ttatagcggt tttacattga
     8581 tcgaaatgct aatcgtctta ctgatcatca gtgtattagt gttgttgttc gtccctaatt
     8641 tgtcgcgtta taggaatcat gtagaccaag aaagtcgtga agcgatcatc cagttggtag
     8701 acacacaaaa agagctctat gcgcttcaaa ataatggaag agttcctact gtggaggaat
     8761 tgttgaatga aggctatatc aaacgagaac atgcagaaat ctatcagcgg ccttgaaggt
     8821 aaggttttag catcaggata tacgttgttt gaatcactct tactgatttt attacttaca
     8881 acgatccttt cttttccagt tctcgcattt tctgcatgga agaatgaatt ggcagtctat
     8941 caatttttcg gccaatttga aaaacgaatc tattctacgc aaaaaatcgc tattgtgaat
     9001 cagatccaaa caggctttta ttggaatagt gaagagaatc aaatcatttt tcatgttccg
     9061 aatccagata aaataaattg ggaagtatta gatattcctg atgagattac cttgaaacgc
     9121 catgcttcta ttacttttgc tgctagaacc gggaatgaaa gttcactaaa agcttatcaa
     9181 ttttattggg aagaaaaaga gcggacgatt acgtatcaat tccagatagg gagtggtcgc
     9241 tatacaaaaa gaattgaata aaacaggatt tgttttgttg gagtccctta cagcgcttat
     9301 gatcgcttct gtctgtgtct acttagtgag ctttggccag ctgtttttgc ttcaggcaga
     9361 aaagaaaagt ggggaggaac tgctatttat tcgaatgatt tatgaagata tcaagacgca
     9421 gcggatgtat cacaaaagac cagagcgaag ggaagaaatg atcccatacg atatccaaat
     9481 tgtcgataat aatggagtaa tcgaaaaagt gtttgtcaat atggagaaag aagcgatcag
     9541 tattgaaaaa acaagtaatt ccagcattta cgctgattga atgtctaatt ggactgatga
     9601 tattgagcag tctattgctc acattcaatc ttttgattca gcaaaccatc caagtaagtg
     9661 aatttttgca gcgtaaagat caaaaagagt ggctaatatt tttagcacaa ttagaagaag
     9721 aactcaagag cagtcacaat gtctctgtga aaaatcagca actgcactac acgatcgagg
     9781 ataaacagta tatcatcgaa agatatcagg caatgatacg taaaagaaga acttccggcg
     9841 gacatcaacc tatgttaaca agtatcactg agcttcaatt attggaaaaa gaacacacaa
     9901 tttcttttta tgtacacttt gaaaatggag aagatggata tgccatatgg acaaaaaacg
     9961 atcagtagaa aatgttataa gggctctatt ttattttctg tcttgctttt atttctttta
    10021 tgtacgtttt tattgatggc agcattagaa agctaccgtt tgtcaatgga tcttaatata
    10081 cgaatcaaaa attattatat agcaaaaata atgaagcaga tggctttatc ggaacagtct
    10141 acgcttgccg aaagttcgga aggagttttt tactacacaa ctggatctgt aacatatcag
    10201 tgggtacaac agtccctatt cttagaggtg gaagtctcac cgtttatctt tcggttcaca
    10261 gaagaagtaa aaaatgatac agatactagt acagaatgat ggaatgttcg tgaaaacgcc
    10321 ctcttttctg ttataacact gtttttaata aaaaaatgtt tgccgtttca caaataaaaa
    10381 acagtgaaaa cgattgaaat tttgtcaatt gttcgataga attaaacagg tgaaaaaatt
    10441 gttataatat atctagaaat gaggtgcacc atgttaccag aaaaaatgga acaagggttt
    10501 aatcaaaacc tggaagctat tcagctttta cagaatgcat tgggaacttc ctttttagaa
    10561 gcctatgtcg aaaatgcaga aaatttaata gatgattatc aagtacgtgt ggtggatgga
    10621 gtgccgacaa aagagacaac gcaacggatc acagctcttt atgaagagtt gaagaaactt
    10681 tctttcgagc cagaagaatg gcgcagactg tctcaattac ttttattgaa gggcagccag
    10741 acagaacatt tgcaggcgaa ccatcaactc actcctgata gtatcggttt tttatttgtt
    10801 tttcttattg aacaactata cacaaataaa aaagcaccgg ttaagattct ggatattgcg
    10861 gcaggaatgg gcaacttgtt attaacagtt cttttaaacc tatcgaatgc ggggtaccaa
    10921 acagaaggaa ttggtgtgga tattgatgat acattgttgg ctgtcgcagc ttctacaagt
    10981 gatttgacac aagcaaatgt tcaatacttt catcaagacg ggttgcagga attattaata
    11041 gatcctgtcg attttgcaat cagcgatttg ccaatcggtt attatccgaa tgacgaaaaa
    11101 gcaaaagagt ttttaacaag taccgaagaa ggccatagtt atgcacatca tttattgcta
    11161 gagcaatcaa tgaaatatgt gaagccggat ggttttggtt tgtttttgat gccgagtgga
    11221 tttttagaaa cggatcaaag tgaagaaatc aaaaaatggt ttaaagaaga aggttatctt
    11281 caaggaatga ttcaattgcc agatgaattg ttccgtaata aacaatcgca aaaaagtatt
    11341 ttgatcttac agaaaaaagg accacaagca caacaagtaa aagaagtctt gcttgtgaaa
    11401 cttgcttcat taaaagaacc agagaaggtg acggaatttt tcaacgagtt taaaaactgg
    11461 aaatcatctg ctctgtaatc attcaacgag aaggagagtc atatgtctaa aacaattgca
    11521 atcaatgctg ggagttcaag tttgaaatgg caattatatc aaatgccaga agaaaccgta
    11581 gttgcgaaag ggatcgtcga acgtatcggt ttacaagatt ctatttttac catcaagtac
    11641 ggcgataatc aaaaattcga acaaatcatg gatatcgaaa accacgaagt agcagtaaaa
    11701 atgctgttag atcagttgat tgaactgcaa attttaagct cttatgatga aatcaccgga
    11761 gtagggcacc gtgttgttca cggaggagaa acttatgggg attcagtagt gatcgatgag
    11821 aaagtaatgg accgcattgc tgaattagca gaatttgctc ctttgcataa tcctgcaaac
    11881 ttaatgggga tcaaagcgtt taaaaagatt ttacctgaca ttttgagtgt ggctgtattc
    11941 gatacatctt tccactcaac aatgccaaag caaaattatt tatatagctt accggtgtct
    12001 tattatgaag atttcaaagt aagaaaatat ggcttccatg gcacaagtca tcgttatgtg
    12061 tcggaacgtg cggcaaaatt attgaacaaa ccgatcgaag aactaaaaat catcacttgc
    12121 cacttaggaa atggtgtatc gatcacagca gtcgatggag gtaaatccat ggatacttct
    12181 atgggattca caccattagc tggggtgaca atgggtacac gttcaggtga tatcgatcca
    12241 gctgttctcc catatttaat ggaaaaatta gagattgata ttgatgaaat gatcaatgtt
    12301 ttgaacaaga aatctggtct gctaggccta acaggattgt ctagtgatat gcgtgatctt
    12361 gaaaagaact atgaaaaaga acatattcgt ttagcttatg acattttcgt ggaccgcatc
    12421 cgaaaataca tcggcggtta tgtgacaacg atgaatggtg ttgatgcaat tgtctttact
    12481 gctggtatcg gcgagaatga cggacatgta cgtagtgaaa tcatcaaagg tatgtcttgg
    12541 tttggttgtg aaatcgatcc agcgttgaat gaattgcgtg gtgaagaagt agatatctct
    12601 acaaaagaat caaaagtaag agtattagtg atcccaacag atgaggaact aatgattgcc
    12661 cgtgatgtgg agcgtctgcg cggctaagcc aaagcattcg ttaggataaa agatgaaacg
    12721 catgcaagag ggaattcttg catgtgtttt ttttgcggag aagaccgcaa aatggtaatt
    12781 taacttcaat ttcccttctt ttttacagga tcttcttagg gaattttgct tggaaaagtg
    12841 gtcaaaaggg aaaagactgg cattttattt attgattttc aggaaaatcc caatatttta
    12901 ttttcattca tctttataag ggacattaca tatgtaaaaa gaaaaaacat ttttagatca
    12961 gcctcgtggg ctgtcgactt tattcttcgc tttatgttta gctagaaaac cgatccgaaa
    13021 cattcattga tcgaataata aaaacttgat tttccacctg tactttgcct gaatcgtgaa
    13081 agcacaggta tgaaaatcaa gttttttgtt gcgaaaaaac gtcctagtca atcagttggc
    13141 cgagcggctg gcggctgatc tgctcctgat caataacgag tgcttcttgg aagtgttgct
    13201 gcaaaaagta gcggtcatgg ctgactgcta ggagcgttcc ttgatagtct tgcagcattt
    13261 cttcaatttc ttctcgagca tagctgtcga ggtgattggt tggttcatcc aaaatcagga
    13321 cgttgatttt ttctaaaaag agtttcagca acgccaaacg gattttttca ccaccggata
    13381 aatctttgat ccgtttaccc acatcgcttt gataaaagcc acttttggct aattgccgtc
    13441 gagcgacttc ttcgtttcca gtcaatgtga tggcgtaagc cagcaaacgg ctatcgggat
    13501 cggcaaactg cagctgctgc ggcaaataac cgatttgcaa gctactacct aatttgatgg
    13561 tgccaccgta aggcttttcc tcaccgagta ataatttgag taaggttgat tttcctgcac
    13621 cattgtcgcc gatgatggct aaatgatctc cacgatagac tgcaaaactg ctgtctttga
    13681 atagtacttg atcagcgtac tgctggtgca agttttgaat gatgaaaact tctttgccag
    13741 attttccacc attagacaag ctttgcagcc gatttttcgg gggaggtggc ggtttgacga
    13801 gtgtcagttt cgccaggcgg cgttccagtt ctttggcttt tttgaagaag gactcattgt
    13861 ctccttcatg tgcccactga cgaaaacgac gaatcatgac tttcaaccgc tggacttctt
    13921 tttgctgcag ttcgtaattt tttgtcagct cagccaagcg agcttctttc aagactcgaa
    13981 aacggctgta gtttccggga tactcaatca atgcaccttc ttccagttcc catgtttttg
    14041 tggtgacttg atcgagaaat tgccgatcat gagaaacgac cagaaccgat ttttttgtat
    14101 tctgtaaata gttttccaac cagcgaatac tgttaagatc taaatgattg gtcggttcat
    14161 cgagtaaaag taaatcggca tcggctgcta aaattttggc taaagcgact ttaactcgtt
    14221 cgcctccgct aagttctgtt aaccgtgctt cctgacagtg tggaatccct aagccttgca
    14281 agatcgctgc aatccggtct tctaatacgt aaccgcctag ttcttcaaag cgatgctgca
    14341 gctcaccgta acgggccaga cacttctcta agtttgtatc tggttctgtc atcaatgctt
    14401 cgtagcgact catttgctgt ttgaggtcct gtatctcagc ggcagaatgg agcaagtagt
    14461 ggttgacggt cgcttcggaa actgtaagtt cttgttcgac cgtcccgatc tttaagcctt
    14521 ttttgcgact gataacaccg ctgtcgacac cttctctgcc ggtcaagatt tgtaaaaagg
    14581 ttgatttgcc agttccgttg atatcgacta aggcaatttt ttcaccagct tcaatttgac
    14641 aggataaatc ctcaaataat ggattcccgc cgattgtttt tttaatagaa attgcttgta
    14701 tgatcatgat ctctctcctt gagaccactc aaaaaggcca tggttatccc tggcctgtgt
    14761 tatttaaact cagattatca tgagaaaaat accttgagat ggccattgat tttacgtgaa
    14821 ttcaactgtt ttggacaaac ttgtcccgtc aacagcaact gtaaaaccaa ttgcatggct
    14881 aaatgcgata tggtatcgtt ggtaattttc attacttgtt attttagtca tgcgcatcat
    14941 agtattttcc ctccttcgtg tagttgtctt ttccagtgta cctgaaagtg tcagaaagtt
    15001 caactagcgc tgacatttac ttaatattta tcgatgtgta attctgtcag agagcttttt
    15061 ttatctgact gagggtaatg tctcgtggac cacatcattg tattctgagc taaaccagcg
    15121 ttcaggcgaa atcatatggt gtttgataaa aaactttgtc tacatgactt ccttttgcca
    15181 tttgcttaat accgttgcat taaacagctc ttctctttgg aaatctcacc aacatcgcgt
    15241 tcaggagtat cgttgtggtt gcaatggcaa gcggtagcct ttcatcaatt cttgaatgat
    15301 ttaccgaatg tagcacttgc cttctcgcgg tacccgaacc cgaaataaaa ttttagtttg
    15361 gcgataaaat cggtatccgc catcgatact tcatattttt tcgtatcaga ttcatcggaa
    15421 acttccacat tgacgaacca ataagctttg gcatgcttag gtcgttttaa accacaccag
    15481 ggcgactaat cgccctggtg ttcacttttt ttattttact tgaactcatt gactgttttc
    15541 cctttatttt aaacattata aagaccgatg agtgcttgcc gcgtcttttg tttttatcac
    15601 ggttttttgt gcaaaaaaac ctgccgatag ttggcaggtc gtctacgtgt taagctttgg
    15661 tgtggtcttt agctggcaat tggttttcta aatcagttcg gacaacaagc acatcgcaag
    15721 ctgcgtttct aataacatat tcagaaacag aaccaataaa taaacgttct acagcattca
    15781 atcctgtggc tcctaacata atcaaatcta cttgattatc ttctgggatt tctctggcaa
    15841 taatttgttt tggtgagcca tattctacga cagaggttac attattcaag ccagctttct
    15901 tcgcattgct ttcataatct gccaatgttt gctttgccat ttcagtagct tgttctgcaa
    15961 gcattccatc aaaggatgaa acggtttgga aagaacgtgt gtcaatgaca tgtgctaata
    16021 gaagttctcc gttatttcgg atagctacat tgaccgcctt tttaaaagct aattcagctt
    16081 catcagaacc atctacggca accatgattt ttttgtattg ttgtaacata agcattcact
    16141 cctcaagtca atcgtattgt actcttttat tgtagaacac tttagggaaa aagagaatta
    16201 tttactgctt tggttcagca attttttttg caaatcggat actgaaggtg gcagaaacaa
    16261 taattacgat cagtaaaaag accaaggcaa taaagcgttg attgaaaaaa gctgttaaaa
    16321 tacccattac gcctaaaaca gcatatgcag caccgtaaaa ttgtaggaat ccttgatttt
    16381 tatcattttt gttgatcaat acaaaaaaga tatcctgttt attaaataaa taatagctaa
    16441 cgaataataa tactaaagtg gcgataatca ttaaaatcct aatcatgaac aaatcctcca
    16501 aaataagcaa caaatagaaa aagcaatttg ccaagagcaa attgctttat tctttaaaag
    16561 atggtaatct ccgttaatgc cgttgtttgt cctgtagtat tgatcccgcg aattcaagca
    16621 ggtgggtccc atggtcgtag cttcgaacta ccaaatgata aggcatgtta ccagcagtga
    16681 cacaagacgg gtcccaagat atcaataaaa atgttcggtc aacacacaaa gaggacaatt
    16741 cgaacatcac agatttccca cctctatagt agcatatgaa tttaaaaaga gcaatgagag
    16801 agctcgcaga aacagaaccg ttttcttgtc tagttttttg gaggatgctt tttccactct
    16861 tttatacggt aatactgttg acccagagcc tgttcatatt tccctgtatc ttttggctga
    16921 taatactgag cattcttcag tttatctggt aaatattgct gattgaccca agcctgatca
    16981 aaatgatgag gatactgata acccacgcca cggttgagtg atttcgcacc tttgtaatgg
    17041 ctatctctca aatgatcagg aacatctcca gctttgccct ctcgaatatc agcaagtgct
    17101 gcatctaatg ccatgtaggc agaattggat tttggcgaca agcaaagatc gacgacgacg
    17161 tctgctagtg ggatacgtgc ctcaggcaat cccaattttt cagcagctaa tacagcgttc
    17221 actgtacgag ccgcagcagc aggattacct aaacctatat cttcataacc gataaccatc
    17281 agacgccggc aaatacttgc cagatcacct gcctcaacta aacgggcgag ataatgaagt
    17341 gcagcatcca catcacttcc acgaatcgat ttttgaaaag ccgaaataac gtcataatga
    17401 gcatctccat ttttgtcatg ggtaagagct tttctttgga cacattcttc aatgatcgac
    17461 agtgtaagat gaatccttcc ttctttatct gaaggagtcg atagtgtagc cagttccagc
    17521 ccattcaatg cactccgcaa atcgccgttc gttgcacgcg aaaggtgaag aagagcatct
    17581 tcgtctaact ggattgcttg ttgtccaagg ccgcgttctt tatcccttaa agcatgttcg
    17641 actgccaact ggatgtcttg ttctgttaat ggtttgactt caaagatctg tgtacggctg
    17701 cggatcgctg gattgatggt aatataagga ttctcagtag tcgctccgat caagatgatt
    17761 cggccacttt ctaaatgagg aagcagaaaa tcctgttttg ttttatctaa tcggtgtact
    17821 tcatccaata agaggatcac tgtaccactc atttttgctt cttccgctac aacttgtaga
    17881 tccttttttg aatctgtagc ggcgtttagc atgcgaaatg cataattagt agagccggcg
    17941 atcgcacttg cgatactcgt ttttccagta ccaggaggtc catatagaat catggaagag
    18001 agcatgcgag catcgaccat gcggcgtatg atttttcctt ccccgactag atgctgctga
    18061 ccaacgactt catcgatcgt tcgaggtctc atgcgataag ccaatggctg ttgcatagga
    18121 ttttcctcct tcttgttttt acgtccctta attatatcat atttagcaag atgcctagaa
    18181 agaacagcct tctgttgttt tgttttcata gcttatccaa ctaatattgt gtatgataaa
    18241 gtaggtgaga aaaatgacgg ataaacaaaa gatagatgaa tttttttgga ataaaacgac
    18301 gccagaagtg gcacgggatt tattggggat gtatttggag catgacacac cagaaggaag
    18361 attagctgga tatattgtag acgcggaagc atacctaggt ccagaagacg aggccgccca
    18421 tagctatggt ttaagaagaa caccgcgtgt gcgggcaatg tatgaaaaac caggtacgat
    18481 ctacctttac acgatgcata ctcatcggat tttgaacatc attactcagc cagaagggat
    18541 tcctcaagga gtaatgatac gtgcaatcga acccgccgca atgattgatc aaatgagtaa
    18601 aaatcgtggc ggaaaaactg gtccagatat cagcaacggt ccgggaaaat tagtggaagc
    18661 attagcaatt cctcaagaac tttatggtca gtctatagca gatagttcgt tacgcttagt
    18721 ttttgagaaa aaaaagacac caaaaaagat caatgctttg cctagaatcg gtatcccaaa
    18781 caaaggtgta tggacagaaa agccgttgcg ttttgttgtg tcgggaaatc cttatatctc
    18841 tttacaaaga aaaaaccaaa tcgagaaaga ttggggttgg aggaaagaaa atgaaaaaga
    18901 aggatctatt aacatattta gatgaaataa ttgaaaaaca agtgacagac tatgatgtag
    18961 ctttagattg ggatacaaaa aatcatacga tcgagatcgt tgttcgcttg tttgcagaaa
    19021 ataaagcaca atttgagatt gatgatgcag aagggatcgt ttcgcaagaa ccaattatcg
    19081 aatttgaaga cggcgtactt ctttttaatc ctcagaaatc tgtttttgat gaacgcaatt
    19141 atctagcagt aatcccttat gaaggaaaaa aagggttggc aaaatcagta gctgattctt
    19201 tagtggaata tctaaatgaa gtattagctc aagggcaaag cgatttgctt gattttttgg
    19261 atgaagacga tgacgaagct gtattcgagc ttcactggga taatcaacaa ctagcagaat
    19321 tagtagaagt aaaacagcaa aatgaagcag atacttattt accgtaccca agttattaag
    19381 gagtgacaga tatgaaatgg aatgaagtaa aagtagaaac tgaaagtgaa gcagtcgaag
    19441 cagttgctaa tattttgatg gaggcagggg cgagtggtgt cgcgattgaa gattctttag
    19501 atgttgaaca ttttcagtcg gatcaatatg gtgagatttt agataaaaat gattttacat
    19561 cgctgaaaga aggcgcacta gtcatggctt attttccgga aacgactttc ttaccggaaa
    19621 tcttgccttt tatcaaagat cgtatcacac aattacctga gtttggtcta gctattggaa
    19681 aaaataaagt aacagttagt gaagtagagg aaagcaactg ggcaacagcc tggaagaaat
    19741 actatcatcc ggtgcgtatt acgcgaatgc tgacggttgc gccaagctgg gaaaactacc
    19801 agacagaaga tcctctagaa aaaattatca cactagatcc tggtatggca tttggcacag
    19861 gcactcatcc cacgacttcc ttgacattga cagcactaga aacagtcttg cgtggtggtg
    19921 aaacattgct agatgtggga actgggtcgg gcgtgctaag tattgcagca aaatatcttg
    19981 gtgcaaaaga tgtctatgcc tttgatttag acgaagtagc cgttcgttcg gcgaaagaaa
    20041 atatggatat gaatgaaata gccaaagatg tccatgtatc agctaacgat cttttgacag
    20101 gaatcgagat tgaaagtgat gtaatcgttg cgaacatttt agcagatatc atcttattga
    20161 tgatcccaga tgcttggcgt ttactgaagc aaacaggcac gctgatcgtt tcaggaatca
    20221 ttgaagagaa gaagcaaatg gttctggatg ccatgttaga acaagggttt atcgtggatc
    20281 aaattttcca gcaaaaagac tggtatgcaa ttattttgaa aaaaccagag gtggaataga
    20341 tgcagcggta ttttttaaaa gaatcatatg aatcaaaaga ctattttgtt ttagaagggg
    20401 aaaactatca ccacattgta cgggttatga ggatggaacc cgaggatgaa gtttttttgg
    20461 cattcaatga tcaaatagcc attgttgcag aaatcacgga gattactgaa gaggctgttt
    20521 atctgaaaga aattaaaaaa gaagaaaatc aaaaggaact gccaagttcg attaccatag
    20581 cgagtggcta tcctaaaggg gaaaagctgg attggatcgt tcagaaatcc actgaattag
    20641 gagcgcaagc ttttcttggt ttcccggcat cagcctctgt cgttaaatgg gatgcaaaaa
    20701 aacgtatgaa acgacaacaa cggcttgaaa agattgctca agaggcagcg gaacaatccc
    20761 atcggcagat cgtgccatca gtagagttat tggaaacgaa acaagattta tttgagcggc
    20821 tagatgatta tgacgtggtc ttggttgctt atgaggaatc agcaaagcaa ggcgaaaaat
    20881 caaatgcagc caaaattctt agtgaaacaa aaccagatag tcgaatctta gctgtctttg
    20941 gaccagaagg tggattatcc cgcgatgaag tcgattattt tgtagaacac aaagggatac
    21001 tttgtggatt aggaccaaga atccttcgta cagaaacagc acctctttat ttattaagtg
    21061 ctgctagcta tcaatgggaa ttaatgaatc cttctttcag ctaacgttct tcattaataa
    21121 aagagtatcg acagaaagaa ttgaaaaaat agtcaacaat ttgtataata gaaacaatta
    21181 tttatacaat ggatacgaaa aatcgtttga aaaagctttt aaaaagcgaa catttccgta
    21241 aaaaaacgta taactgcacg gattttggga caattatcaa acagatattt gtcccgattc
    21301 ccgttttacc gaaaaaagaa agggggatcg attcctgtga caaaagaaga aattttaacg
    21361 ggacccggtg tcataaagct cgttagtcaa tacatgggtc ccgaacatgt agcatttgtt
    21421 gagaaagcat gcgaatacgc gactgctgca catgacggac agtttagaaa atcaggcgaa
    21481 ccctacatta ttcatcctat ccaagtcgca ggtatattag cagatttaaa aatggatccc
    21541 catacagtgg ctacaggctt cttacatgat gttgttgaag atacagaaat cactttagaa
    21601 gatctgagag aagaatttgg cgatgacgtt gctatgttag tggacggcgt aaccaaatta
    21661 gggaaaataa aatataaatc ccacgaagag cagctagcag aaaaccaccg aaagatgctg
    21721 ctagcaatgg ctcaagattt acgagttatc atggtcaaat tagctgatag actacataac
    21781 atgcgtacgt tgaagcacct gcgagaagat aagcaaagaa ggatcgctca ggaaacttta
    21841 gaaatctatg cgcctcttgc acatcgtcta gggatcagcc ggatcaaatg ggaattagaa
    21901 gatacagcac ttcgttatct aaatccaaaa cagtattacc gtatcgtcca cttgatgcag
    21961 acgaagagag aagaacgtga aaaatacgta agtggtactg ttgaagatat tcgaatagcg
    22021 acagaagagc tggggatttt tgcagaaatc tatggacggc caaaacacat ttattcaatc
    22081 tatcgtaaaa tgaaagatca gaaaaagcag ttcaacgaaa tttatgactt gctagctatt
    22141 cgagtgatcg tagattcaat caaggattgt tatgctgtat taggagcaat ccacacaaaa
    22201 tggaaaccaa tgcccggcag atttaaggat tatattgcca tgccaaaagc taatatgtac
    22261 caatctttgc atactaccgt gatcggtccg gcaggaaatc cggtagaaat tcaaatccga
    22321 acacaggaaa tgcatgaaat cgctgaattc ggggttgctg cacactgggc ctataaagaa
    22381 ggaaaaaatg aaaaagtaga accagatggt atgacgaaac aattaagctg gttccatgag
    22441 atactcgaac ttcaagacga aagctatgat gcttctgaat ttatggaagg cgtaaaagga
    22501 gatatcttta gtgataaagt ctacgtcttc acaccaaaag gagacgttac tgagttacca
    22561 aaaggatccg gaccattgga ctttgcatac agtatccata cagatatcgg taacaaaacc
    22621 actggtgcaa aagtaaatgg caaaatggtg cagcttgatt acaaattgaa aaacggagat
    22681 atcattgaga tcatgacttc tccaaattca tttggcccaa gtcgcgactg gttgaaatta
    22741 gttgctacta gcaaagcaag aaataagatc aaacgtttct tcaaagccca agatcgagaa
    22801 gaaaatgtga tcaaaggcca cgaatccgtg gtcaaatgta ttacagatct aggatttacg
    22861 cctaaagata ttttgacgaa gaacaaactg caagaagcac tcgatcgttt taattatcaa
    22921 acagaagatg atctctatgc agctgtaggg tatggagaag ttagcccctt gacgatggcc
    22981 aatcgtctga ctgaaaaaga acgtaaagaa caaaaaatcg agcagcaaaa gcaagaagca
    23041 gaagaaatca tgaatcagcc gaaaaaagaa cctgacaaaa tgaaagtacg tcatgaaggt
    23101 ggcgttgtca ttcaaggggt agaaaactta ctaattcgta tcagtcgctg ctgtaatccg
    23161 attcctggtg atgatatcgt tggttatatc actaaaggca gagggatatc cattcatcgt
    23221 cgagattgtc cgaatgttca gcctgacaaa ccaaatgtag cagaacgttt gattgaagtc
    23281 gaatgggaag atacatcgaa tacacgaaaa gagtatgatg cagatttgga aatttacggc
    23341 tataatcgtt caggcttatt gaatgatgta cttcaaacag tcaatgcgct aacgaaaaat
    23401 ctcaacagcg ttgaagcacg gacgaataaa gataaaatgg cgacgatcca tttgacggtt
    23461 ggtatccaga atttatccca cctaaagagt atcgtggata aaatcaaagc agtacctgat
    23521 gtctacagtg tacgccggac gaatggatag gaggacagat atgagagcag ttgttcaacg
    23581 ggtaagtaaa gctgctgtgt cagtcgatca gcaagaaatt ggaaaaattg gctacggcct
    23641 acttgtttta ttaggcattc atgaaaccga tacgcaaaaa gatgtggact atctggtaaa
    23701 aaaaattgca caaatgcgta tttttgaaga tgaacaagga aaaatgaata ttagtgtaga
    23761 agatgtggga ggacaaattt taagtatctc tcaattcaca ctgtttgcgg atacgaaaaa
    23821 aggaaatcga ccaagctttg tggaagccgc acgaccagaa acagctattc ctttatacga
    23881 atcattcaat gaaggaatca gaagtcgcgg catcacagtc gctaccggac aattcggagc
    23941 agatatgtcg gtttctctcg tcaatgatgg acctgtaacg atcattattg attctaaaaa
    24001 caaataaagt aatctggttt tcactagtaa aaactatgaa acacgtcgct taagttagac
    24061 tgataaactc taacttaagt ggcttttttg tctgcaaaaa gcgcttcttt tgtcagcagt
    24121 cggaaaaaaa gttaagctta aattatacaa gttatttttt ataggaggtt gctgaagatg
    24181 aaagaagtac cagtagtaga tatcaaaatc acagatttaa aaaaattgta tcaaaaagga
    24241 tacaacatgt tggaggagtt acgccatgaa gcggacgctc ccgtcgtcaa agcaaagatt
    24301 ttcaataaag aagctatcac gatctacgga tcatcagcag caaaagtatt ttatgatcca
    24361 aggaatttca aacgaaaagg tgctatgcca aaattagtat tgaagacact ttttggtcaa
    24421 ggtggagtac agactttgga tggagctgct catcaccatc ggaaaaatat ttttatggat
    24481 ctaatgacac cggaaagaat ggaagattat catcgtatct tagataagaa tctgacacaa
    24541 gcacttgaag cacagcatgg tcaatttgaa ctatttgatt taagtaagat ggttttcttt
    24601 acatctattt gcgagtgggc aggtatcaat ttatcagcga tttctaaaga tgaggtcgaa
    24661 aaattagcag aatatcaaat ctctatgatc agcggaacgt ttacttctcc gattgatcac
    24721 ataaaaggtg tcgagaatcg caaaaaatca gaaaaatggg cgcaaggctt gattgaagaa
    24781 gctcgacaaa atcctgtggc tggaaaagaa aatgtggcat tatatgcttt tgccaatgcc
    24841 acagatttag atggtcagct tttacctctg gaagtggcag ctgttgaatt attgaacatc
    24901 atccgtccaa cggttgctct aactgtgtgg gcggcattga tgggacatgc acttttcagt
    24961 agaccagatt tgtatcagca attaaaaaat gacttttcga cgttacaaga tccgtttatt
    25021 caagaaatga gacgctatta tcctttcttc ccaatgctac ctgcgatttc gctgaaagaa
    25081 gtagaagtag atggttatcg tataccagaa ggcagttggg ttattttaga tttatatgga
    25141 acagaccatg atgaacgaac agttgaagcg ccagattcct ttatgatcaa acggtatgta
    25201 gggaaagcaa aagatatttc ctataaagaa gaatatgaga tgattgcaca aggcggggga
    25261 aattttcgac aaatgcatcg ctgtgcagga gaatggatca ctcttcatag tctacgtgtg
    25321 ttttccgatc agctagtaaa taagtttgag ttctctgtac ctgaacaaga ttggacgatt
    25381 ccattcaatc agtttccgac ttatccgaat agccgagcgc tactttacaa gaattagatt
    25441 agcataaact tatcgaaaaa aaggaagatc aaacagtgct gatcttcctt ttttcatatt
    25501 actataaatt gataaggtca attgatcaaa atccacgaaa tgcttcatca atggtttgat
    25561 aatcttcatt ggataaatgc aaatctaaag cttggacatt gtcagatact tgctctggac
    25621 gcttagctcc aggtataact acagacactc gtggattctt catgtaccaa gctaaaacaa
    25681 gttgcgcagg agtggcttgg tatcttttag caatggggcg aagtacatcg acagcggtta
    25741 aaatttctct gaaacgttct ccttggaaat ctggatttct ttttcgagga tctccttcac
    25801 caaattgttt ttcctcacca agttcatatt ttcctgtcag caaaccggaa gcaagaggaa
    25861 aatagggaac aaaactgatt ttgttttttt caagataagg gaataattct ttttcagcct
    25921 gccgatggat caaactgtat ttatcttcta ctacatcgac atatccatct gcattcgctt
    25981 ctttcaattg ttctaacgtg aagtttgaca caccgactgc tcggattttt cctgcttctt
    26041 ttaattcgtg caatgtagca actgattcat tttttggtgt gctttcatcc ggaaagtgga
    26101 tataaaaaat gtcgatgtaa tcagtttgca gacgttttag tgcatcttcc accgattgtt
    26161 tgagaaactc aggagaatta tcaaatgttc gccctttatt tggtacatga gcagcttttg
    26221 tagcgatgac gacacgtgag cgatcatatt ctttttcctt cagtacctcg ccaatcagtt
    26281 cttcggaacg gccgtttccg taagcataag cggtatcgat caaattgatc ccgctattca
    26341 aagctgtacg aactacttgt tttcctgtct catcttccaa accgtcaaaa agattatgtc
    26401 ctccaacggc atttgctcct aatccaagct gttctgcgta gacttgtgaa tgtccgattc
    26461 ttatttctgc catagctgat tcctcctttt tgtctagtct aaaacaaaat accaaaaagc
    26521 tgaactaaaa cgtttttgtg aggggaaata gtcagatatc tcttgacttt gagactggtt
    26581 tttagtaggt tataagtaat atcgaatgtc aatgaaagag cgagtaagtt tttgaaactt
    26641 tacaagagag gatcatcgag gctgaaaatg atctaaagtt tcattagcct aaagacactc
    26701 tggagatgga cgagtgaaaa gccgtcccgc gtacgagcgt tattcgtaag aggaaagaga
    26761 aatctttcaa aattaggtgg taccgtgtat gaatttcatt cacccttgtt catgatgaac
    26821 aagggtgatt tttttattta catagctaag aaagggagag aaaactatga gtttccaaag
    26881 accaaaagga acaaatgata ttttgccagg cgaatcagaa aaatggcagt ttgtcgaaga
    26941 aacagcacgt ttattattca gagattatca gtataatgaa atgcgtacac cgatttttga
    27001 acactatgaa gtgatatccc gaagtgtagg ggatacaacg gatatcgttt caaaagagat
    27061 gtatgatttt tatgacaaag gggaacgcca tgtgacgctt cgaccagaag gtacagcacc
    27121 aatcgttcgt tcttttgttg agcataaatt gttcggccca gagtatgcga aaccgttcaa
    27181 aactttttat atgggaccaa tgtttcgcta cgaacgccct caaaaaggac ggttacgtca
    27241 attccatcaa atcggagtag aagcatttgg atcagaaaac cctgcaactg atgtggagag
    27301 catggtgatg gcacttgatt tcttcaaaca gttggggatc acgcaaatcc ggctagtcat
    27361 caattcatta ggtgataaag aaacgcgtca agcctatcgt caagcattga tcgactatct
    27421 tttacctttt gaatcagagc tgagtgaaga ctctaaacgc cgccttcatg aaaatccatt
    27481 acgcgtattg gacagtaaag acaaacgaga tcaaaaattt gtggcagaag ctccatctat
    27541 cttagattat ctaagcgagc ctgctcaaaa acattttgat accgttgttt ccatgctgga
    27601 tgcattggct gttccttatg aaatcgacag taatatggtt cgcggactag attattacac
    27661 acatacgatt tttgaaatca tgagtgacgc accaaaaatg ggggcacaag caacgatttg
    27721 cgcaggcgga cgttatgaca atctagtgga agaactaggt ggtccttcaa cacctggctt
    27781 cggatttgca atgggtatcg agcgattact cttgactatg gaggcagagg aagtagttat
    27841 ccctgcattt aatgagttgg atgcttacgt ggtcgcactc ggagacgaaa caaatatcga
    27901 agcattgaaa gtggtccaag cgattcgaaa ctttggtttt tcagctgacc gcgacttcat
    27961 gaaccgtaaa gcaaaagcgc aatttaagac agcagataaa gcgaatgcca aattagtatt
    28021 ggtgatcggt gaagatgagc ttgctagtca aacgattaat gtcaaagcaa tggcaagccg
    28081 aaaagaaaaa agttttccat tagaagagat atatgaacat tttgatgaag tctatgatga
    28141 aatgacttta ttcgagtagg aggaaaaaaa taaatggcac aaagaacgat ctattgcgga
    28201 ttagtttccg aacaacaatt agaacaaaca gttacattaa aaggatgggt acaaaaaaga
    28261 cgagaccttg ggggagtaat ctttatcgac ctacgtgatc gtgaaggaat cgttcaaatc
    28321 gtctttaatc ctgaaatcaa caaagaagca tgggaaatcg cggataaatg tcgcagtgaa
    28381 tacgtgatcg aagtgactgg ggaagttaga agacgcggag aaaatgcagt aaatcctaaa
    28441 atggccactg gtgaattcga agtgattgca agtgaaatcg tgattttgaa tcaagcaaaa
    28501 accccgccat ttcaaattga agatgatcaa gcaatcagtg acgaaatacg tttgaaatat
    28561 cgttatttgg atctgcgtcg tccaaaaatg actaaaaaca tcaaattaag aaatgcgaca
    28621 acaaaagcga ttcgtcatta tttagatgat aatgactttc tcgatatcga aacaccttac
    28681 ctagggaaat ctacaccaga aggcgcaaga gactacctag ttccttcacg tgtccatgct
    28741 ggacatttct atgcgttgcc gcaatctcct caattgttta aacaattgct gatgaatgct
    28801 ggattcgacc gttattatca aatcgttcgt tgtttccgcg atgaagattt acgtggagac
    28861 cgtcagcctg aatttacgca agtagatatt gaaacaacgt tcttaacagc agaagaaatc
    28921 caaacttaca cagagggatt gattgcgaag gtaatgaaag aagttcgcgg tatcgaagtc
    28981 acattgccat ttcctcgtat gacctatgat gaagcaatgg ctcgctacgg cagcgacaaa
    29041 ccggatacac gttttgatat ggaattgatc gatctaagcg atacagtcaa agaagtagaa
    29101 ttcaaagtct tccaaatggc tcttgaaaac ggcggtgtcg tgaaagcatt gaatgctaaa
    29161 ggcgctgcag atcgctactc acgaaaagac atggatcagt taggtcaata tgtaggacaa
    29221 ttcggagcta aggggcttgc atggctgaaa gtagaagaag acggattgaa aggtccaatt
    29281 gctaaattca tgggagaagc aacagaagca atcattaaag caacagatgc aaaaccaggt
    29341 gacttgctga tgtttggcgc agataaaagt gaaatcgtcg ctgctgcatt aggtgctatc
    29401 cggacacgtt taggaaaaga attaggacta attgatgaat cgaaattcaa tttcttatgg
    29461 gtaactgatt ggccacaatt tgaattttca gaagaagaag gtcgatatgt ctctgctcac
    29521 catccattca cgatgccaaa agaagaagat atcccgttat tggctgaaga ccctgcaaaa
    29581 gtctatgcag aagcttatga tatcgtcttg aacggttatg aattaggcgg aggttctatc
    29641 cgtattcata ctcgtgagct tcaagaaaaa gtactagaaa ctctaggctt tacaaaagaa
    29701 caaatggaag aacaatttgg tttcttgtta acagctttag attacggatt ccctccacat
    29761 ggcggtattg cattaggatt ggaccgtttt gcaatgttat tagccggaga agataatatt
    29821 cgtgaagtca ttgcctttcc taaaaacgga aaagcagcag atgtgatgag tgatgcacca
    29881 agtatggttt cgcctttaca attgttcgaa ttgaatattg atgtaacgaa tatagaagaa
    29941 tagccgataa aaaggtaaaa aggatttgtc gaatacgctt cttttgacaa ccttgattta
    30001 attgaaaaac actttataat gaagccataa catataaagc ggggcttttc catgaaaaaa
    30061 acattcgaaa attgggctta tcatgattac acgaccaagc tttcggtatc tatcgtttat
    30121 gcgttgatcg catcagtcgc attgaatttc ttttatcagc caggacatat ttattcaagt
    30181 ggtattactg gtctagctca gattttgacg actttgtcaa aatcactgat aggattcgaa
    30241 gtaccggtat caacaacttt gtatttactg aatatacctt tgtttgtatt ggcgtggtta
    30301 aaaatcggga agaagtttac aattttcact tttatcacag tcactttgac ctccctttgt
    30361 atccatattt taccggaaac agtcctatct accgatccaa tcatctgtgc gttgtttggg
    30421 ggggctgtga tgggactagg tattgggcta gcgttgagaa acggcctatc atcgggcggt
    30481 ctagatatcg tgagtatcac gattcgtaaa aaaacaggaa agtcgattgg ttctatttcg
    30541 atctacttca atgcattgat cgtttttgtt gccggaattc tatttggctg gcaatatatg
    30601 ttttacagta cgttgtctat tttcgtcagt ggaaaagtaa ctgatgtcgt ttatactaaa
    30661 caaaagaaga tgcaggtaat gatcgtaacg aaacatccag aaaaagtagt aaatgaaatc
    30721 caaaactgta tgcgtagagg gatcacgatc atcaatgaag cagaaggtgc ttataagcat
    30781 gataagcaga cagtattgat gacgatcgtt acacgatacg aactgcctct acttagagaa
    30841 gcaatgaaag cttctgatcc acaagctttt gtaagtattg cagaaaacgt ccagatactt
    30901 ggtcgtttct atgaggatgg catttagatc gagtttgaca aaaattcatg aaatactgct
    30961 gtagacagac gttcgtttgt ctgcagtttt tttctaccat agtagaatca gcaaagaaaa
    31021 actgcttttt tatttgaaat tttcttaaag ttgaaaaaaa gcatgtttat ttttttaaat
    31081 ttatgggatg atagaaaaaa atagtagaga aaggacacga ttctacttgc tattttcact
    31141 taatacccgt atattacgaa tgaaaaaagt ttatgaaaag ttggaatatt gaatggaaaa
    31201 acaaaaaagg ttaaaaaaca gccgctacat cactgggttt gatgggctcc gttcattagc
    31261 tgtggtgggt gttatcttct atcaccttct gccaactagt atgaaaggcg gctatttggg
    31321 cgttccgatt tttttcgtcg tatctggtta tttgatcacg gatctgctgc ggcaggaatg
    31381 ggaacaaaat ggaaaaatca atatttggca attttatgtc agaagaatga agagactcta
    31441 tccggcactt gtttttatgc ttgtgacagc ttcggcttat atcactttgt ttcagcgagg
    31501 gctgttaaac aatcttcgag gagttgtggt cagcagctta ctgtatgtca acaactggtg
    31561 gcaaatcaat aacggtttat cttatttcga tcgatttgct aatgagtcac cgtttacgca
    31621 tatctggtct ttagcagtag aaggtcaaaa ctatttggtt tggccgatct tgtttgtatt
    31681 gttgatggta tttgttcgta aaagaaaatg gatcttttat actgtgttag gtacatcctt
    31741 cctctccgcc atcttgatga tgatcttgta tacaccagga ggcgatccaa cccgtgttta
    31801 ttacggtacc gacactcgtc tgttttctat atggttggga agtgcgcttg cgtttgtttg
    31861 gccaagtacg cgtttaaaga aaaacatccc gaaacaagcg aaaaatattc tgaatatcgc
    31921 tggaggaatc tcgcttttgg cactgattct ttgcttcttc tttttagatg atcattacaa
    31981 tttcatctat tacggaggaa tgctcctagt gagtctgtta tgcgtagtgc tagttgcagt
    32041 gacagcacac cccggagcaa gtttgaatcg atggctgaca aatccagtat ttacttggat
    32101 tggcaaacga agctatggca tttatctgta tcagtttcca gttatgattt tttatgaagc
    32161 aaaggtgact gatattgccg atcatgtttt gttgcatact ttagtggaaa tcacattgat
    32221 tctaatcatc agtgaacttt cttatcgata tgtggagcgt ccgctagcac gttttgatta
    32281 tggtcgtaca ttccaagtga tgaaaggatg gttcagcaaa ccgatcatca gtaagagcaa
    32341 accatgggtt gttccaggca cattacttgt agcaattgca ctcgttggtt tcgttacggc
    32401 accgaaaaac gctgtaacag ccgatcaaaa acaactgcag gcaaaaatcg ctgaaagtaa
    32461 aaaattagca gaagaaacca gaaacaattc agaaaacacc actagttcgg ttgaccaagc
    32521 gatccttgat aagtacgagt tgtccaaggc acaagggaat aaggcacaaa aattagaact
    32581 aacggcattt ggtgattcag ttatgttgga tgctgcaacg gatctacaag aactttatcc
    32641 aaaagtagtg gtagatggag atgtcggacg acaattatat gccagtgtac cgtacattga
    32701 aaaattaaag gaacaagatt tgttgaaaga cactgttctg attggactag gaacgaatgg
    32761 ctcattctcg gaagctcagt ttgatagtgt gatggatgca atcggtgatc gaaaagtata
    32821 ctgggtcaat gtccgagtac cgacgaagcg ctggcaaaat gacgtgaatg cgatgcttga
    32881 acagatgacg aaaaaatacg acaatatgac gctgattgat tggtacagct acagtaatga
    32941 ccatagtgac tggttttatg atgatcaagt ccatccaaat cctgaaggga tgacacatta
    33001 cattcactta gtcagcgaag ccttgctagg aggaagttca agtacgaaca gcagtgagac
    33061 aaacgtgaca gaatgatagc tacaaaaaac ttcaggcaaa aacatttgtt tttgcctgaa
    33121 gtttttttat atttaccgtg tttttaacat tcatatagat tcaggaagca aaacggttga
    33181 gtgtgtctgt aaataacgtt ctttttgttt tcgggtgagt tttttgaatg cagcttctgc
    33241 ttttgttgct tcgcttcttg aagaaaatac ttctgcatgg atcatcttaa caggtcttct
    33301 tttttgaaga cgagtatatt tagcaccagt accattgtta tgttcctcta atcgtctaga
    33361 aagatcggtt gtatatccac cataaaatgt ctgatcttta cagaataata cgtaaaaata
    33421 atgttcattt gccatagagc atctcacgaa tctctgcagt gtattctcca tccatattgt
    33481 aagtgatcaa tggtggagcg actttgaatc cgtctaattt gccttctttg attccttcaa
    33541 ctaacaaaat atttgcctct tttccaggtt ttgggtaaac aaattggatc cttttaggtg
    33601 caattcgata gtcttccatg acctgaatga tttctaagaa ccgatcagga cgatggacca
    33661 tagtaaagcg tccatttgtt tttagtagct tacttgaaat atcgatcact tcttctaacg
    33721 tcgtatgaat ctcatgacgg gcgattgcca ggtgtttatt agggttcgtt ttatttgttg
    33781 gcagcccttt gaaataaggt ggattacaga cgactaagtc acaagaatta tgtcgaacga
    33841 cagataagct gtctttcaga tcgatggtgt ggacagtcat ttgttcattt aaatggttaa
    33901 gcgcgatact acgtcttgcc atgtctgcta gacgaggttg aagttcgata gcgtcgatag
    33961 ccgcctgggt tcgtttactt aaaaaaagtc caactgcgcc atttcctgca caaaggtcaa
    34021 caatcttccc ttttttaggt acacgggaaa agtgagcaag cagcacagca tctaaagaaa
    34081 aagaaaacac tttggaactt tggataatct tgatatcttc tgcgtataat tggtcgattc
    34141 gttcgtcttc gtgaagcatg gattccctcc aaaatgaaaa aaatagtcgt ctgtattata
    34201 ctatttttat taaaaagaac caaggtggct tgtcaaacct ctatttttta tgcatactat
    34261 gaccataaga agggaaagga agagactatg ttttatcgat ttatgcgcgg ggtagttcga
    34321 gtactattag ccttagttaa tggaaatgcc cgctatgaga ataaagaagt tttaccgcaa
    34381 gatgagaatt acatactagt tgctccccat cgtacttggt gggacccatt atatctggcg
    34441 gtagctgcag cacccaagga attcagcttt atggcaaaag aagaactgtt caaaaatcca
    34501 gttctgcgat ttatattaac tcgatccaat gcttttccag ttaaaagaga aaatccagga
    34561 ccaagcgtca ttaaaacacc tgtaaagatt ttgaaatcga caaatttgag tttgatcatg
    34621 tttcccagtg ggacaagaca tgctacagaa cttaaaggcg gaatggcatt gatttccaga
    34681 atgtctaaag cgaaaatcgt gccagctgta tatcaaggtc cgttgacact tggggatctg
    34741 ttcaagcgtc agagagtgac agtgcgtttc ggcgaaccaa tcgacttatc agatatcaag
    34801 aaaacggata aagaaggtat ggcggagatc gaacgacgaa cacaagcagc ttttgacaaa
    34861 ttggacaaag aagtgaaccc tgatttcaaa tatgaagtca aaaaaaaata aaatgataag
    34921 tttatgcgtg ggcggtcttt tgaatctact gtcctgttca gttcagactt tttcaatgaa
    34981 aacgagggag agacgtcaag tctgccctct ttttgcgttc caatatgtaa aatagaagac
    35041 aggtgtcatg taatggatca aagtgttata ataattagaa ggacattttt tgaggtgatt
    35101 gaatgcgttt tttacataca gccgattggc atatcggaaa aaaactacaa ggttatgact
    35161 tgattgaaga ccagcgtcat gtactatctt ctatactaga tatcgcgaaa gaagaaaaag
    35221 tagaggcaat cgtgattgcc ggagacttat atgataggag tgtcccagct gttgatgccg
    35281 tagaattata caatgaattg atggtcgatt ggaatttaaa ggaaaagatc ccgatttttg
    35341 ctatatcagg aaatcacgat agcagtacga gactttcagc gggtacgcca tggttcagtt
    35401 attcgaacta ttttttgcga acacagctag cagaagcatt tgaaccagtc gagtttaaaa
    35461 acgtacaatt tttctttctt ccttactttg aaccaattgc agcgagatta tattttgaag
    35521 atgacaatat ccgaacgatc aagcaagcgg tagccaaggt gatagataaa atgcaggagc
    35581 tatttgattc agaaaaaaaa caagtattga ttagccattt ttttgtaatg ggaagtgtaa
    35641 aatctgattc ggaaacgaaa atcgaagtag gcgggttgga ttcgatccct ggtgaactac
    35701 ttacaccgtt tgattatgta gcactaggac atttgcataa tcaagctgct ttgagacagg
    35761 ataatgctcg ctatagcggt tccccattga aattctcttt atctgagatc aaccaaaaaa
    35821 aaggcgtatg gatcgttgac ctgacaacag gcctggagtt gaattttagg gagatcgaac
    35881 ccctgcatga tattgaagaa ataacagcga gtttcaaaga attgctggat cctgcctttt
    35941 atcagtccgt caatcgggag aattacatct atatctatct cactgataga acgatcatcc
    36001 cgaatatgat gaaccagctt cggcaaatat atcctcgtat tttggggatt gaacgggcaa
    36061 acggtcgagc agaacaaaca aagcgcaaac gaaaagtact agaaatgaaa gatcctcaaa
    36121 aattagcaga agcatttttt agtgaaatga cggaagagca gctaactgca aaccaagaaa
    36181 aatggctcgg agaaacgttg caagaaatca tggaaatgga atgatcaaga atgaaaccga
    36241 aaaaattaca acttaaaaat tttggtcctt ttatcaatga gaccgtagat ttttcacgtt
    36301 tgacagaagc accgcttttt ttgatcagcg ggaagacagg agctggcaaa acgacgattt
    36361 ttgatgggat caccttcgca ctttttggtg aaacttccgg aagacttcgc tctggaaaag
    36421 agatgcgttc tttgtttgct acaccagacg aagaaacaag cgtgactttt tcttttgaac
    36481 atcagcagat gagctatgaa atcgaacgaa aaccagaaca agttttagca aaacgaaaag
    36541 gaaacggaac aaaaaaacag gcagccaaag taacgttgac catttttgat gggaaaggaa
    36601 aagaaataaa acaaatcaca aaacgtacag aagcagatca gttgatcaga gaactgatga
    36661 atctcgatgc gaaacagttt tctcaaatcg ttttactacc ccaaggagaa ttccgtaatt
    36721 ttttggtttc atcaagcagt gataaagaga ctgttttacg tcatttattc ggtactcaat
    36781 ttttccaaca gttcaatgag cgattaaaag aaaaagcaaa acaacagcaa caaaaattgg
    36841 atcatttaga gcaagagctg catcttctgc aaaaaaggtt tgttttagtc aaagaagagg
    36901 aaccagcagc agggtttgaa gccgttttag cccaatggac aaaccatcag gcaatactcg
    36961 aaaaacaaat cacggaagaa aaagaagctc taacggaact tcaggaaaaa caaaagcaac
    37021 tagaaaattc ctactatcgt tttgaaagct tgaaaaaaag ttatgaagaa aaaacgatat
    37081 taataaacaa gcaaaaggaa ttagctgctc aaaaagacga aatagaggaa aagaaacgat
    37141 ggattgggta ttatgaattt acgcaacgat tagtcgaacc aatccagcgc ctacaagaaa
    37201 taaaagttga aaaaatagaa ctgtcacaaa aaagtagtga gcaaacaaag aaactagcga
    37261 tgactacgca gaattatgaa gagtggcaaa aaaatgaagc aaaccgcgta cgccgacaaa
    37321 caaaaatcaa agaagctgct caaaaactgc aaaacaaaca gatgatggtg ccaattgtcg
    37381 aatccttacg tagtaaaaaa caagacgcag aacaattaac aatttcattg gataaagaag
    37441 aattgaatgc cgagaagctg tatcaaaatc tcgctatcta tcaggaaaga actcgaattc
    37501 ttcaagaaaa tttactggat caggaaaaac tgcaagaaga tcgtcttgct tttggacagt
    37561 taaaatatct agatgaaaag cagcaagaaa tagaaaaaca aaaagcaaaa gacactgggt
    37621 cgctcgcaca gataagagaa gaactcaaaa aagcaactga agaaataaca aaaaatagtc
    37681 agctattgaa agaacagaca gaacaagcag aacagctgaa aagccaatgg gcaaagcagc
    37741 aaattgctcg tcttcagctt tttttgcgtc caggggagcc atgtcttgtt tgtggatcaa
    37801 cggaacaccc catacaacaa ttaaaacacg aacagccaaa taaggaagat atcttaaaag
    37861 tagaacaaca actggcagaa gctgagtctg ctgtagaaag aactcagcaa atcattgcca
    37921 aaagtgaagc acatgaaaat aatcttcaac aacaattatc agatttagaa caaaaaatca
    37981 tggcaacgaa tatacaattg gctgaacaag aagaacactt gcaacaggct ttgaaaaagg
    38041 catttcctga gataacagaa caaaaaacaa aaacaactct tgtagtaatc gaacaatctt
    38101 tagatagacg aaaaagcaag atggccaatg aaagaataga gctggaagaa ataagacaac
    38161 aagcagaaaa gctcgaacaa caattagctg tctacaaaca gcagcagaca gaaaaaaata
    38221 atcagctcca gcaactgcaa ggagaaatca aaagtttaga acaacaattg caagatacgg
    38281 atagtaactg tttagaagag gagatacgac gacttgaaaa acagttagaa gaagactcga
    38341 atcaactagc tgttgagcag aaagcaggag tgcaattgtt gcaagatctt catacgcttc
    38401 ggacacagct tgatcttcaa catgaacaaa tagaaaaaat agatgaaaaa atagaaaagg
    38461 aacaagaaag aatagctaaa aagttagaaa gccagtcgta tttttcaaca attgaagaaa
    38521 tctgtgcatt tgcacaacag caagcacaat ataaagaaaa ccaacgtaca gttacttctt
    38581 atcatgaaga gcaattagtg acagctgacc ggctgcgtca attagaaaat atccagctaa
    38641 ctgagacatt cccagataca gaagagatca gccaacagtt agaagaaata agacgtatga
    38701 tcgaaataaa gcaagaaaag atatacggtt tacaagaaca aaaaattagt aatcaaaaaa
    38761 tctacgatga atgtctttcg atttatcaca caagccagac gaaattagat gagcttacgc
    38821 aattacagca gctttcgcaa acattcaacg gagaaaatcc taagaagact agtttggaac
    38881 ggtatgtgct tcaggtttat cttcaagaag tgttacaagt agcaaatgat catctccaac
    38941 gtttaacgaa aaatcgttat caatttgagc tagcggatac cattggcagt tatcgaggga
    39001 aaaccggatt agaaatcaat atctatgatg atcaggcagg tatgtctcga agtgcgcata
    39061 ccctgtctgg cggtgagagt tttattgcag cactttcttt agcattagct cttgccgatg
    39121 tgatccagac acaagctggt ggtgtagcaa tcgatgcatt gtttattgat gaaggatttg
    39181 ggtcattaga tgaagaagct ttggaaatgg caatggaagc tttagaaacg atcgaaagtg
    39241 aaggaagaat gatcggcatc attagtcatg taagagaatt gaaagaacga gtattacaac
    39301 aaataagaat tgatacagaa ggtagcggac aaagcaaaat acgctatcaa cttggataaa
    39361 aagggagatt ggtaactatg aaatggagta ttccagaaaa agtcattgca cgtggacgtc
    39421 aatacttgca agaaggccga gtgttatcgg ttgtccctga ccaagaagaa aacgtgtggc
    39481 atgcagaagt cttgggcagt gaattgtata tggttgattt ggatggtaca gcaaaagaga
    39541 tagattactg ccaatgtccc tattgggagg aacataaata ttgtaagcat acagttgctg
    39601 tggaacttta tttacggtcg aaaaatctat ctcgtatcat gaaaaaagac cagccaatgc
    39661 tagaaccggt aaaagcaaca agtgagggag aaatcattgc aaaaggtttt tctcgtctaa
    39721 aaacacaaaa caatcctcaa gcggtgcaac ctttgattat tgaataccaa gtagaaacga
    39781 ttgagactaa tcagtatcac ccagagcttt ctattttggc actttacttg cgtatcggtt
    39841 cattggcatc accgaaaaaa acatatatcg taaaaaacat ctatgatttt ttacaagctt
    39901 acacagaaaa agaaacttac actgttaata aacagtacca gtttcgtcta gataaacggg
    39961 cgtttcaaaa agaagatcaa aaaatactca cgcatcttgc aggaagtgct cagacgcatc
    40021 aattattagg tacgaacggc ttacaagtca aaggaaaact agataaaaga tatctgcttc
    40081 tcccagtaga acaagcccgc ttcctgttag agcagatgaa tcagactaca cgtttgcatc
    40141 tgcagataaa tgaacaaaac gatcaaggaa tacaattttc tgatcaaaac aaacctttat
    40201 cattcaaagt ggaaaaattt ggagaagatt atttgctgca agcattgaat gattttgatt
    40261 ttttctttat gcattatcag tggggagtta tccaagggac attttattct ctgacgaaat
    40321 atcaacaaac ggtttatcaa acttggaaac aattgattcg ccgattggag aaaccagaag
    40381 tagtgttcaa gaaaaaagaa ttaccgtctt tgtttaaaga gattatccct ctgctgtcag
    40441 agatcggcga cgtagctatt gcccctgcag tagaagagga agtagcagat atccccgtcg
    40501 aatttatttt ctttttccga aaagcaaaag gaaaaatcca agctcgagtc gatttcgtct
    40561 atggtgaagt catctattcg actgacccga agcatgaggt ctcgacagaa caggcttttc
    40621 aggtgttacg tgacacagta gaagagaaac gagtacttga tctattcaga atgtatcgct
    40681 accagaaaaa tgaaactggt tatgaacgtc cattgccaag cggggaagaa ctatatgcct
    40741 ttttccgcac agaactatct gtcttccgtc agtttggtga agtcagactt ggaagaaaat
    40801 tgcgagagtt gtatttagat gcgcataaac atcaaccgaa aatcgaagta accgagaccg
    40861 gttcatggct cgatattcga ttcgatgtga cagggataga ggagcaagaa atcgatcatg
    40921 tcctgcaaag cttgctgaga aatgatgctt tttatacatt agaaaatggt caggttcttt
    40981 cttttgattc agaagaattt caacaaacaa gccaagtgtt gcaacaatta cgggaatcga
    41041 tacgtacaga agaaggaacg atccatgtac ctaaaaatca aggattgatc attcaaaatc
    41101 aattggaaaa aagcaatgct acttttagtg aatcgttcca aacgatggta caagatctca
    41161 ttcatccaga aagataccag gcacagcttc ccaaaggatt gaatgcaacg atgagagatt
    41221 atcaaaagca aggttttcga tggttgaaga tgttaggtca ttatcagttt ggcggtattt
    41281 tagccgatga aatgggactt gggaaaacac tacagacaat tgccttttta ctttcggaaa
    41341 aagaagaacg gaaaagcttc tctgctttga tcgtagcgcc tgcgagctta atttacaact
    41401 ggcaggcaga ggtgagaaaa ttcgcccctt ctttaagtat ccaagtaatc aatgggaata
    41461 aaaaagaacg tgaagaatta ctggctaaag acacagatat ccgagtgact tcttatgcca
    41521 gcttaagaca ggatctagct aattatcagt cgcaaaaaat tgattatttg attcttgatg
    41581 aggcacaaat ggtcaaaaat agcagtacga aaactgcaca agcattgaga gaattagctg
    41641 tacctcagcg gtttgcactg agtgggacac caatcgagaa taatttagaa gaattgtggt
    41701 cattgtttgc aacgattatg ccaggatttt tcccgactaa aacaaaattc agggaacttg
    41761 ccgcagaaga aattgcgcaa atgattcgac cgtttatttt acggcgagat aaacaaactg
    41821 tgttgaaaga cttgccggaa aaaacagaga tgaatctata ttctgcactt acagaagaac
    41881 aaaaaactgt ttatttagct tacttgcgtc aaatgcgtga agaaatcaca tcaatggatt
    41941 ctgaagcatt caagaaaaac cggatcggga tacttgctgg tctgacaaga ctgcgacaaa
    42001 tttgctgcga tcctcgtctg tttattgaag attaccaagg cggttccggc aagctcgaac
    42061 aagtaaaaga tctactggta gctgccaaag agaacaaacg aagagttttg ctgttttctc
    42121 aatttacagg gatgctgact atcttgcaag aagagttagc cgagctaggg atctcgacat
    42181 tttatttaag aggcagtacg aaaccacaag atcggttaag tatggtagac gcatttaatg
    42241 ctggcgaaaa agatgttttt ctgatttcct taaaggctgg gggaactgga ttgaatctca
    42301 cgggagcaga tacggtcatc ttgtatgacc tttggtggaa tcctgccatt gaagaacaag
    42361 ctgctggacg tgcacatcgg atcggacaaa aaaatgtggt agaagtgtgg cgtatgatct
    42421 ctgaaggaac gattgaggaa cgcatggatt cactccagca agaaaaacgc gaactgttcc
    42481 aaaaagtgat ccaaggtaat gaagaacagt tgacgaaact gactgaagaa gacatccgca
    42541 tgattttaag tgtaggagaa atcgaggagt aagagactcg aaaaagcgtt cagtaatggg
    42601 taagaaagcc tttctaaaga agcgattgtt tatttgattt aaaaaatagc aaggaggctg
    42661 ggattccatt ttatcccagc ttccttgcta tcattttttt atttctattt ttaaaggtta
    42721 tacaaagatt gtcttcattg tctctaacag ctgatcaaaa gaagaaatct cgatatcgca
    42781 aattggagtc tgttcaattt tgcgttctcg atggttgaac caaagcgctt gccagttagc
    42841 actttttgca cctagaacat cattgtcgta attatcgcca acgtataagg tctcttctgg
    42901 aagcatatga aattctttct cagccagctg aaaaatctct ttttctggtt tttggaaacc
    42961 tgttgcttga gaaatgagca tattgcggga aggaatccaa ttgttcaatt gaagttgctt
    43021 cagttttttt gtctgatggt cagtcggccc atttgtgata attcctaatg ggacattctt
    43081 ttccttcaag aaatcgaggg tttttttgac ctcctcatgc aagcagatat tatcaagttc
    43141 ttcctcgtaa attttttgaa ataagaggcc atcttcttcc gtaatatgag ggtagtctaa
    43201 atcttttagt gattggctga tccgatgtgc tcgcatatat tctagtgtcc aatctcctgc
    43261 cattactttt gggaagtttt catcactatg atggcggaac cggatatata gtggatgcat
    43321 atctgcatct gaaacaagcg gaacgactct tttgaccgcg tttcgaaatg gctgctgctg
    43381 atcatacatc gtatcatcaa catcaaacac aactgattta atcaaaattt tacacgcctt
    43441 tcggttttca ttgatttttt cacaaatcct tgactattgt aacagaaaaa attgtctaaa
    43501 aaagctgata aataaaggtt tttcttacta tatttctatt tccttgacag aataaggttt
    43561 ttatatttca gtttttttca ctaaaaatcg tgaaacacgt caataagttt gatgaacaaa
    43621 ttaatcatta tggtagaagt cgattgattt attttaatgc aatctctgtg gaattttcga
    43681 aagatttatt ttt
//
LOCUS       DAJSBM010000017        40992 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJSBM010000017 DAJSBM010000000
VERSION     DAJSBM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.017"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(278..1060)
                     /locus_tag="ONT67_001106"
     CDS             complement(278..1060)
                     /locus_tag="ONT67_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354861.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1125..1961)
                     /locus_tag="ONT67_001107"
     CDS             complement(1125..1961)
                     /locus_tag="ONT67_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2354862.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1979..3388)
                     /locus_tag="ONT67_001108"
     CDS             complement(1979..3388)
                     /locus_tag="ONT67_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2354863.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3402..5225)
                     /locus_tag="ONT67_001109"
     CDS             complement(3402..5225)
                     /locus_tag="ONT67_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2354864.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6068..6394)
                     /locus_tag="ONT67_001110"
     CDS             complement(6068..6394)
                     /locus_tag="ONT67_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354865.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6440..6802)
                     /locus_tag="ONT67_001111"
     CDS             complement(6440..6802)
                     /locus_tag="ONT67_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR2354866.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6839..7054)
                     /locus_tag="ONT67_001112"
     CDS             complement(6839..7054)
                     /locus_tag="ONT67_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354867.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7181..7516
                     /locus_tag="ONT67_001113"
     CDS             7181..7516
                     /locus_tag="ONT67_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354868.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7592..9589)
                     /locus_tag="ONT67_001114"
     CDS             complement(7592..9589)
                     /locus_tag="ONT67_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354869.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9687..9962)
                     /locus_tag="ONT67_001115"
     CDS             complement(9687..9962)
                     /locus_tag="ONT67_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354870.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10194..10928
                     /locus_tag="ONT67_001116"
     CDS             10194..10928
                     /locus_tag="ONT67_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354871.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10967..12109
                     /locus_tag="ONT67_001117"
     CDS             10967..12109
                     /locus_tag="ONT67_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2354872.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12204..13595)
                     /gene="sufB"
                     /locus_tag="ONT67_001118"
     CDS             complement(12204..13595)
                     /gene="sufB"
                     /locus_tag="ONT67_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR2354873.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13600..14070)
                     /locus_tag="ONT67_001119"
     CDS             complement(13600..14070)
                     /locus_tag="ONT67_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCR2354874.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14057..15292)
                     /locus_tag="ONT67_001120"
     CDS             complement(14057..15292)
                     /locus_tag="ONT67_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2354875.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15289..16575)
                     /gene="sufD"
                     /locus_tag="ONT67_001121"
     CDS             complement(15289..16575)
                     /gene="sufD"
                     /locus_tag="ONT67_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR2354876.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16588..17361)
                     /gene="sufC"
                     /locus_tag="ONT67_001122"
     CDS             complement(16588..17361)
                     /gene="sufC"
                     /locus_tag="ONT67_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR2354877.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17558..18406)
                     /locus_tag="ONT67_001123"
     CDS             complement(17558..18406)
                     /locus_tag="ONT67_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR2354878.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18430..19116)
                     /locus_tag="ONT67_001124"
     CDS             complement(18430..19116)
                     /locus_tag="ONT67_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354879.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19109..20143)
                     /locus_tag="ONT67_001125"
     CDS             complement(19109..20143)
                     /locus_tag="ONT67_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2354880.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20206..20462)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20538..20876)
                     /locus_tag="ONT67_001126"
     CDS             complement(20538..20876)
                     /locus_tag="ONT67_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCR2354881.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20878..21234)
                     /locus_tag="ONT67_001127"
     CDS             complement(20878..21234)
                     /locus_tag="ONT67_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCR2354882.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21240..22427)
                     /locus_tag="ONT67_001128"
     CDS             complement(21240..22427)
                     /locus_tag="ONT67_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCR2354883.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22618..23463)
                     /locus_tag="ONT67_001129"
     CDS             complement(22618..23463)
                     /locus_tag="ONT67_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2354884.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23697..24662
                     /locus_tag="ONT67_001130"
     CDS             23697..24662
                     /locus_tag="ONT67_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCR2354885.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25039..26082
                     /locus_tag="ONT67_001131"
     CDS             25039..26082
                     /locus_tag="ONT67_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCR2354886.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26144..26608)
                     /locus_tag="ONT67_001132"
     CDS             complement(26144..26608)
                     /locus_tag="ONT67_001132"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR2354887.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            26854..27801
                     /locus_tag="ONT67_001133"
     CDS             26854..27801
                     /locus_tag="ONT67_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2354888.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28012..30084)
                     /locus_tag="ONT67_001134"
     CDS             complement(28012..30084)
                     /locus_tag="ONT67_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2354889.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30017..30394)
                     /locus_tag="ONT67_001135"
     CDS             complement(30017..30394)
                     /locus_tag="ONT67_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCR2354890.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30523..31101)
                     /locus_tag="ONT67_001136"
     CDS             complement(30523..31101)
                     /locus_tag="ONT67_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2354891.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31233..32525
                     /locus_tag="ONT67_001137"
     CDS             31233..32525
                     /locus_tag="ONT67_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2354892.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32585..33703)
                     /locus_tag="ONT67_001138"
     CDS             complement(32585..33703)
                     /locus_tag="ONT67_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCR2354893.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33717..35138)
                     /gene="arcD"
                     /locus_tag="ONT67_001139"
     CDS             complement(33717..35138)
                     /gene="arcD"
                     /locus_tag="ONT67_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCR2354894.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35384..36505)
                     /gene="mnmA"
                     /locus_tag="ONT67_001140"
     CDS             complement(35384..36505)
                     /gene="mnmA"
                     /locus_tag="ONT67_001140"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR2354895.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            36909..37508
                     /locus_tag="ONT67_001141"
     CDS             36909..37508
                     /locus_tag="ONT67_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCR2354896.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37646..38932)
                     /gene="efmA"
                     /locus_tag="ONT67_001142"
     CDS             complement(37646..38932)
                     /gene="efmA"
                     /locus_tag="ONT67_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCR2354897.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39203..39547)
                     /locus_tag="ONT67_001143"
     CDS             complement(39203..39547)
                     /locus_tag="ONT67_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCR2354898.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39684..40829)
                     /locus_tag="ONT67_001144"
     CDS             complement(39684..40829)
                     /locus_tag="ONT67_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2354899.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11779 a         8627 c         7173 g        13413 t
ORIGIN      
        1 attttttcat aaaaaaacag tactgtctag tagtataaaa tatacaaatt tcttaaaaat
       61 tttttcacta aaatcactac ctgaaacagt ctaaaacact tatatatcaa cgtttaatga
      121 aactatctcc gtttcatttc agatacaaat aaatatagtc gggctccaaa tccgaaaaaa
      181 aataccccca gtagcttatc ctaagctact ggagatgtat aagtacggtc atataacaac
      241 ggggtagggt aaataaatga aaaagatgga agaatcatta ttcaataact tttttaaata
      301 cttcgttgta atcagttacc tgtccgttct taaataccac agaataatct tttttcccat
      361 tttcaagtaa ccgaagtgga atgtctttac caacttgctc tgtcactgca ttactgatac
      421 ctgttagatt agctaataaa tctgataaag attgagcatc tgtagaaatc tctgtatcag
      481 tatttatcgt gattccttta tcttcattta caagcttcca agtaggtggt atagctgtgt
      541 atgtttcgat gaatgaatca attctttcat ctatcgtctt cccagtatct acttctgtct
      601 cattactctt agatgaatcg attctttcat ccatcgtctg ctccgtaact gcttctgtct
      661 cattattctc accggaaact ctcttcacac ccaaacttat atattgtcca tctttagtgt
      721 gcatagttaa aattcccacg cttttactaa taaattcact ataaccagtt ctttcaatag
      781 agacttcaat gtatttcaca ctatcatact caaaagaaaa atagacatgt aaaagcattt
      841 tgttgtttac atctacataa taatcctttg actctacttg agaccttcct atacggaata
      901 cagctttact gtctgatatc tctataccag catccttatt attaatctgc caatctccaa
      961 gtagctcttc tggcaagcta gtagtttctt gtgaacactt taccacttca ctggtagagg
     1021 tcgaaacatt cacctccgca ctgttagaag aattttccaa ggaatcagga gccttagcac
     1081 aaccaacaag tctttatata ttgtgcagcc gatattttct tcattcagta cacccttgaa
     1141 atatgcagtg ttagataaag cagttcgtcc tgatttgctg aggcacccat ctcttcttga
     1201 acgtactcct tgattttcaa ggccgcccga tactctttcg gaaaacgctg cttcactgaa
     1261 gccagcaaaa aatcatcctt ctccggcaac tcttccttct taatcattcg ggcaataaaa
     1321 aactgtaaat gcgtcacaaa tcttaagtaa agcatcgaat ctttatcaac ctttttttcg
     1381 ggaaacagat atcctagcat agccaagata tcctttagtg ctcttaccga gagcatggtt
     1441 tcttccatat ttgcttgttc actttgggca tttacaatgt gaaacgcaat gtttccagcc
     1501 tcctcttctg gcaactgaac attcattttt ttgttgatca attggatagc atagcagccg
     1561 atctcaaatt cttttgggta atacttttga atttcaatca gcaggcgatt ttgaaaaaca
     1621 atgccttttt gactgcgttc aatggcatgc ttcaagtgat caaccaaggt aaaaaataga
     1681 cttttattca gcttaaagtc aaacttggcg ttggcataat cgataattcg cttgacgcaa
     1741 ataattagat catccggagc agcctcaaac agtttaatgt aatccttttt tagagcatca
     1801 gaaagtgaat ctaatttaaa tactttttgg attttgtcag ccggaaccaa atcgcctttc
     1861 tttagttgaa atccaatccc tttccccatc aaaattactt ctttgccttg cttatctaag
     1921 caaagaatta aactgttgtt taacgtttta attactctca ttagattcac cgttctctct
     1981 aagctaagcc atttcttgcg attacttttt gataccaaaa gaagctttct ttttttattc
     2041 gttttaattc tttaagattc tcatcggttc ggtcaacata tataaaacca taacgcttat
     2101 tgaagccttg atgggtacta acaacatcaa tcgctgacca aggtaaataa ccaaaaacat
     2161 ctacaccatc tgttaaagct gcttgaatag ccagaatatg tttggctaga taatcaattc
     2221 gatcctggtc attaattcgt ctatcttcct caactatgtc gggtgcccca tagccatttt
     2281 cagtaattaa aataggtaaa tcatagcgat cgtacaattt cctcaatgtt aatcgtagtc
     2341 cagtagggtc aactacccag ccatatggcg ttttatctac ataaggattt tcagctgccc
     2401 ggtagacacc ttcttctccc aacatgatct gttgatcccc cgctctagcg gaaacatctg
     2461 atccgtcatt tttgctggca gcgatggtgg cagttgaata ataattaatc gcaataaaat
     2521 cgggtttacc ggaggataaa attgcctgat cctctgcctc aattttagga tataatccac
     2581 gatcattcaa atagttttca aacaaataat tgtattttcc ccgaactgct acatctaaga
     2641 aagaccagcc tctcaatgtt tcccaattat gggctgcaat tgcatcttct ggactggagg
     2701 tagcttgata catagccgtt aaattgattg ctggaccgat ttttccttta agattcaact
     2761 gatgatacaa gtgaattact ctagcttgag ctaataaaac atggtgatta atttgaaaga
     2821 gttctttttt tgaaggcagc tctccacttg gtggcaaacc aatcgctcca ggatgaagga
     2881 tcatcgtgtt ttgctcatta attgtcagcc aatagttcac cttgtcacca tactcagtaa
     2941 gcaatacctg tacatagtcc acaaaataat cgatagttgc tcgatttaac caaccgccct
     3001 gctcctctag aaaataaggt aaatcgaaat ggtacatggt gacaatcggt tcgattccct
     3061 gattcaggca ttcatcaatt acttgatgat aaaaagaaat tcccttttga ttggttttat
     3121 tattttcaat atccataatt cgcgtccaag cgatggaaaa gcgaaaagct tttaagccta
     3181 attctttaaa cagctgtata tcttctttat agtgatggta aaaatccgag gcaacttcaa
     3241 aattagctac cccttctgta tgctgatact tgtcaataat agacggttgt ttcccatcct
     3301 cctgataggc accctctgcc tggtaggcac taatggaggc accccataaa aacttctctg
     3361 gaaatgctga aagctgtttg tattgcacaa aaatcctctc cttaactaat ttgcatcaat
     3421 ggttcgtgtt cctttaagct tccgcttgct tcactaattc ctgcgtaatt ttcactgttg
     3481 gtcactacca caatcactgt atcatcgtag ccagcgtttc taatggcagc gaaatcggcg
     3541 ttgattaata attgtcctgc tgacacctga tctcctgctt ttacaaatac ttcaaagggt
     3601 tcccctttta aattcacggt gtcgatcccc acatggatca ataattcaat accttcttta
     3661 gaacgtagac caatcgcatg tttagattct aaaatagtaa tgacttctcc ctcaacaggt
     3721 gaataaatgc tgttttcttc tggatgaatg gctacgccct ttcccaatag tcccttagaa
     3781 aagacagcat cgttcacttc agaaatagga cacacggttc cttttaccgg attttcaaca
     3841 atgactgctt cggctattgc cgtttgctct gattcatcag aagtatcgtc aaatccaatt
     3901 agataagcaa tgataaaggt tgcaacaaag gcgattacac acgtaataat tgcgtgaaca
     3961 atattcattg gattttctcc aataaaggct ggtaaggctg ctaagccagg ggaaacaaag
     4021 gcatatctga ctaaaccgct caagccagca tacaaaccag caattgcacc accaatcatg
     4081 gaagcaacta atggtttttt cagcttcaag gttactccat agagtgaagg ctcagtaatt
     4141 cccattaaag cagtgatccc agtggacaca gctagctgct tcaactcttt gtttttagtt
     4201 ttcgcggcta cagcaaaggt agcacctgat tgagcaatat tagaagccag cattcctggc
     4261 ccattgatca tttcagaacc cgtactgctc aattgcatag tagcgattgg cgtcattgcc
     4321 caagctgtac ccgtaaccac caaaaatggc tgcaaaccac ccatcaaaaa tggaattaac
     4381 caagctgctt tgccgttaat ccattcagcc cccattgcta taagatcgtt cagcaacgtt
     4441 ccaaacggtc caatgacaac caacgctaaa ggccccataa ttaaaaatac cagcatcggc
     4501 ttagtaaaga atttaataaa cgaaggcgaa attcgatccg caaagggctc tacataggac
     4561 atgatccaaa cagtaaagat aatcggtaaa actgatcccg cataatctgc agctaatacc
     4621 ggaattccta gaaaagaaac ggcatcgcca gtagaaaaaa gctgtgaaat atttggatga
     4681 agcaaaatgc cagcaacagt aatcgccaaa attgggttga catcaaactt tatggctgcc
     4741 ccataggcta atagtactgg cataaaataa aaggctgcat cagcaatagt attcaaaatg
     4801 atgtaagttt gactactgtc tgatagtaca ccagtcaaag tcaagacggc caaaatggct
     4861 tttaccatcc cggcacctgt tatagctgga atcatcggtg taaaggtggt agaaatgaca
     4921 ctaatcaatc ttgaaataat actttcttga ttgtctgaag aggggccctt tttaattgaa
     4981 gttcccactt ccttttgaat ttccttgaag gctgtttgga cttcattgcc aatcaccact
     5041 tggtattgtc caccgctatc caccacacta attacaccaa gtgttttttc taaggctttc
     5101 gtatcggctt tccttttatc tttgagttca aaacgcagtc tggtggcaca atgggttaag
     5161 ctgaccacat tttcttttcc cccaacctgt tgcaatacat ttcttcctac ttctctgtaa
     5221 tccattattc tctctcctta aagcagtaaa aaagaccaag tcaaaccacg ccccaatcaa
     5281 cattgaagtg ttagtttgtc ttggtcttgc ctgcataatc agtaacatgc caataacgtt
     5341 attttgctgt aatccaataa tagcacatta tgatagcgca atcaacaggg aatttaattt
     5401 tttatctttt cgtttgaccg tatagggtaa tataacagtt agatcacatc aactagggta
     5461 tatctgcgta tacttactgc taaaataagt atatctaata ttaactatac cagtaaaacc
     5521 attcctacat aaccagcggg aaattcccaa aatcgtgaaa gcatcaaaaa tgcatataac
     5581 ggatcacaca acttcaacaa ttctgctctt gggaaaaatt gatgaatttg gtgagtaaag
     5641 gatggaataa acagcattcc aacaatatag gcaacaatag tgatacttgt ttttttggta
     5701 aaaatcagta agcaatagcc aatttcaatc attccgagat ataaaggtat cgatagaatg
     5761 atttgggcta gtattcttaa aaattaacta gcaaatgttt aaactgattt atttctaatc
     5821 tacatgtagt aagtatttca attccaccaa ttatcaataa aaataccaaa catatccata
     5881 ataatttttt ctttttcatt tcttttatat tgttataaca gtgaataaca taaaaattcc
     5941 cgacaatcat tgtaatattt ttttgtcacc tgtattagta tttaatttat aggaaactat
     6001 aaaaactccc tagtgaattt acacctttgg gagttcctgt ttggacggta tgcataatac
     6061 atttaaatca atttaacatt aacacctcag catatggtat ctccacatat atacggtctt
     6121 tctttggata gtactcaata tttatgttga tagcacttcc attgtctaat atagctttag
     6181 cccggtatgt gttatctcca ttttcatgag taatcgtcac atcatcacat gatcgattta
     6241 agttgtactg ttcttgtaaa atctgtgtta ccaatggttt tgatgacttc tctaatccag
     6301 atgtacctgt catttttgaa aaaataatac ctataatagc caagacaaca atacctataa
     6361 tgacaaactt cttgttcata tttactttat tcaacttttt atctcctatc tccaaaataa
     6421 tttgctctta tccctcttat cagtacattg acatccctaa aagtacacca aagattgctc
     6481 cagctacccc tgctatcatc aaaatcgtac ccattttctc atctttttct cgatataagt
     6541 agcccataat aacagcaaca gcaccaaata caacaggaat aaatgctaac gatactacca
     6601 tagaaatcca accaataatc aaatacagct tccctgtctc ttgtccatgt acgatttgtt
     6661 ccttcatttc attaattgtc tgctcagtac ttttctttac tgtgtctgtt ccggttgtct
     6721 catctgtttc ttgtagtgct ccacaaaact ggcaatactt actttcatct gttatttctt
     6781 tgccacattt tctacaatac atgtcttctc ctcctaaaat cattcaattg cttattagtt
     6841 aactgtttac tttattaaat acatcagaaa acagctcatt cattggtaca cttaatatct
     6901 cagaaagcat cttcatttga tagggtttag gaatcgttct ccctgtttgc cacgaactta
     6961 cacattgctg tgtaacattc attttttttg ctaactccgt ttgagaaagt cctttttctt
     7021 ttctgtatcg gctgagatta cttacttttt tcatgattct cccctcctgt tttattatat
     7081 acaacaatat gatgtatttc aactcataaa atataaaaaa cttcaaatac cattacaaat
     7141 acaacaactt gttgtactat actgagatgg gggtgtatta atgaaacaga aacaatatgc
     7201 aaaggtcatt agagaaaagc gaaaagctct cgggtggaca caaaaagaat tagctgaaaa
     7261 aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag agcctaacct
     7321 cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt ttctagacaa
     7381 tgttgtcgta gattatgagg aagagttttt ggctctctat cgttctctta gcactgaaga
     7441 tgcagttcga acaattgact atatgaagct attaaaaagg caagaaaatg aacgcaatca
     7501 gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt gtattgagat
     7561 gaaataagat taagagctat ggataacctg tctagtttaa tacaattggg tcttttctct
     7621 tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgatacaga ggaactatcc
     7681 cagctcctga taatatcaat tcttttattg tttcatctat ccctgcccct ttcgctaaat
     7741 atattaaaaa actactactt gctttcacat tgtatagttt cttaatatcc ttagaagctc
     7801 gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttttattaaa gcatttttca
     7861 cgctgttatc cccaacatta cgtaggcgtt cacaaacagt gttaataagt tctttctctg
     7921 tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct tcatctaaaa
     7981 tcaattgttt ttccttgctg agttctgatc gggaattttg aggaaatact cttttagcct
     8041 ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat gtaaaatgaa
     8101 ttttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa gttgcattaa
     8161 aaacgtctaa tcgatagaca ttgcttctct tatgattaaa gtttcctaac gctttctctt
     8221 cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca tctaattttt
     8281 ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct atgtcataag
     8341 ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa atacaagtca
     8401 cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca tagacacgtc
     8461 gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt gattcaagag
     8521 tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga tacaagtcta
     8581 ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa attagcatca
     8641 caactccaat aaaattttca ctgatatcac aattactgga atgacaataa aaaatggaat
     8701 gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga aacagacatc
     8761 ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat ctttcataaa
     8821 tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac tgtttcattt
     8881 cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact agttcatact
     8941 ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca atcggaagct
     9001 cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac ttatatcctc
     9061 caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg ttctttttca
     9121 agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt aatagaaaga
     9181 caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc gcaaacgcag
     9241 aattatgaac taaattgata gataaaacct tttctacgct taagtatcta ctatcatctt
     9301 tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca tccagaataa
     9361 ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc ttgcttggaa
     9421 taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact tctctatagt
     9481 tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc ttctctaata
     9541 atttttcttg atctacataa attggaatat taatcattga tgtttccatt tgtgtttcct
     9601 ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac tctttattac
     9661 tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa actatcaata
     9721 tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc gtattgagaa
     9781 acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg ctgatagtcc
     9841 atatactcta gagtgactcc agaaataatg tcttctagca taggctttag cactgtaatt
     9901 atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat ttgtagagac
     9961 atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat cttcgtattt
    10021 tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa atgattacca
    10081 gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaacagct ttgtaaaatt
    10141 tatttgcaac accccttttt tgaggtatac taattttagg aggtgtggtc aatatgataa
    10201 aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa ataaaagcta
    10261 taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac aacggtcaat
    10321 taattgacaa aaaaactatc tacgaatggg agaagggaac ctatttacca ataccagagc
    10381 gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt tgtggcaatg
    10441 tagaagaata tattttggga attattcttt atagagatag cattgtacta gatggtataa
    10501 catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca gtacactcta
    10561 atttagatac atggctggat agatattcca aattagaacc agagatgcaa gaatttattg
    10621 ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat atcttaaaaa
    10681 tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt cttttcctca
    10741 ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct attcaattga
    10801 aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata aacaagctag
    10861 aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag ggaggtgata
    10921 ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttaatgg caaaaatatc
    10981 aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca ttgccaccga
    11041 tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca aacaagaagc
    11101 tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa agaaagcacc
    11161 tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta ccagtgttaa
    11221 agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc cagcatttgg
    11281 taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag atgaatggca
    11341 cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt tacgctacgg
    11401 ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc cacgaaaaaa
    11461 agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt tactggatgc
    11521 ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg catacacagg
    11581 tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt ttaataaaga
    11641 actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa ttcaatctcc
    11701 gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt ctatcctcaa
    11761 caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata ctagctctaa
    11821 agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca ctgttaatga
    11881 ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc atgggtttag
    11941 acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag ttcaacaacg
    12001 attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta caaataatgc
    12061 acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa agagctggtc
    12121 aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct tatatatcaa
    12181 gggttctagc tcctataaca ggtttaccca acactgcctt ccatctcata actgatcaat
    12241 cgattgagtt cgactgcata ttccattgga agttctttgg tgaatggttc aacaaagccc
    12301 atgacgatca tttcagttgc ttcagattcg gtcaggcctc ggctcatcag atagtagagt
    12361 tgttcttctg agatttttga gactttggct tcgtgttcta aagagacttg gctgttgtgg
    12421 atctcattga atgggatcgt atcggatttc gaccattcgt ccatgatgat cgtgtcacat
    12481 tcgatatgag agatcgaacc cgcactgttt ttggcaaagg ttacttgtcc tcgatagttt
    12541 acttctccgc cgtcttttga aattgattta gagacgatag agctagatgt atttggtgca
    12601 ttgtggatca tttttgcacc cgtatcttgg atttggtttg ctcctgcaaa ggcgatggag
    12661 agcatggtgc ctcgtgcacc ttttccatct aagtaaacac ttgggtattt catcgttgtt
    12721 tttgcaccta ggtttccgtc gatccattca acggttgcgc cttcgtatgc ttttgctcgt
    12781 ttggttacta agttgtacac attgtctgac cagttttgga tcgttgtgta acggcaatac
    12841 gcatcttttc ttgtaaagat ttctacgatg gcggcatgca ggctgttact tgaataagta
    12901 ggtgccgtac aaccttcgac atagtggatg cttgctcctt cgtcaacaat gatcagcgta
    12961 cgttcgaatt gccccgtgtt ttccgcattg atccggaagt acgtttgtaa tggaacatcg
    13021 actttgacgc cttttggcac gtaaataaag gtcccacctg accatacggc tgaattcagc
    13081 gctgctaatt tgttgtcggt tggcggtaca agttttgaaa agtactcttt gaataattca
    13141 gggtattctt tcaaagcaga gtcagtgtca gtaaagacga ttcctaactt ttcgaattca
    13201 tctttcatat tatggtacac gacttctgat tcatattgtg cggaagcacc ggctagataa
    13261 gctcgttcag cttctggtat accaatcttt tcaaatgtat ctttgatttt ttctggtacg
    13321 tcttcccagt cacgagcggg tttgtcactt ggtttttggt aatatttgat ggcatcgaaa
    13381 tcgatgtctg aaaggtcggg gccccatgtt tgcattggca ttttgttaaa tgtttctaga
    13441 gatttcagac ggaattcaag catccattct ggttcgcctt tgattcttga catctcacgg
    13501 atcacttctt ctgtcaagcc ttctcctgta ctgaaaaccg gttcgacatc atcgtgaaac
    13561 ccgaatttat attcttctaa ttctggtacg cccatgttct cactcctctt ccttgtggta
    13621 atgatggaaa gctgcggtac catcgttttc cattgctttt cctagtgctt tccaggcaag
    13681 tgtcgcacat ttgatccgag cgggaaattt agctacacca ccaagcatag cagcatcgcc
    13741 taattggtcg ggatctttga cttcttttcc ttggacaagc tgtagaaagt ctacagttaa
    13801 atgttctgct tcttctattg attttccgat gacggcgtct gtcatcatcg aggcacttgc
    13861 cgtactgatg gagcagccac tgccgtcaaa ggcgatattt ttgatgattc cgtcttttac
    13921 atcgacttgc aattcgatca catctccaca tgtggggttg ttcaattcga tcttgcttgt
    13981 cgagtcagtc aagcttccac gatgatgcgg atgggaagaa tgatccaaga tcacttgacg
    14041 gtataaattg tctaatttag atagtcccat gttggaaaaa ctcctttgtt gccatgattg
    14101 cttcaactaa acggtcggcg tcttgcgttg tgttgtagaa ataaaagctc gcgcgagcag
    14161 tcgctgatac ttcaaggtac ttcaatagcg gttgcgcaca atgatgacct gctctgacgg
    14221 ctaccccttc catgtctaaa gctgtcgcga catcatgggg atgtaaccca tccaaattga
    14281 atgcaacgac tcctgtccga aaattcgggt cttgtggacc ataaacagtc aatccatcaa
    14341 ttgcttgtaa ttttggcaac acataagcgg ctagttcttc ttcatatcga tggatattgt
    14401 ccataccgag tttttgcaaa taatcgattg catggcctaa tgcgatagcg ccggcgatat
    14461 ttggtgtccc tgcttcgaat ttccaaggta gttctttcca tgtactgtcg tataattcga
    14521 caaaatcgat catttcgcca ccaaattcaa caggttccat ttcttctaac cattttcgtt
    14581 taccatataa aacaccaata cctgttggtc cgcacatttt gtgtccgctg aaagcaaaga
    14641 aatcacagtc tagtgcttgg acatcaatgg ccatgtgcgg ggcagattga gcaccatcta
    14701 ctacgatgac cccgccgttt tcatggacaa gttcagctaa ttcttgaaca ggattgaaga
    14761 cacctaagac attggataca tgagcaatgg agacgatttt ggttttgtcc gtaatctttt
    14821 tcgctgcatc ctccatatcc aggaagcctt cttctgttag ttcgatatat ttcaagacag
    14881 cacctgttcg ttttgccaac tgctgccacg ggataacatt agaatgatgt tccatgtaag
    14941 agagcactat ctcgtctcct gctttgatat agcggtcgcc gaaggttctg gctacccaat
    15001 tcaagcttgt cgtcgtacca cgagtaaaca gcacttctgc tgtttctttt gcatggatga
    15061 acgcccgtac tttttctctt gcttgttcat aatcatgggt cgcgcgttct gctaacgtat
    15121 ggacaccgcg atgaacattg gcgttatcgt gttcgtaata gtacgctaat gcctggagta
    15181 cttgttttgg cttttgggtc gtcgctgcat tatccagata aactaaaggt tcatcattga
    15241 cgtcttgaaa taagatagga aaatcattgc gtagcttttt agcatcgatc atccattcaa
    15301 cttcccttcg attacttcga ctaattcttt ccgaacttgt tctacaggga ttgctgttaa
    15361 gacagaaccg aggaatcctc gaatcactaa gcgctctgct tcttctttat gcaatcctct
    15421 actcatcaga tagtacattt cttctggatc aactcgtcct acactggcag catgtccagc
    15481 agttacttcg ttttcatcga tcaaaagaat aggattcgca tcaccacgtg ctttatcaga
    15541 aagcatcaag acacgacttt cttgttgggc atctgcacct tttgcccctt tcaagatgtg
    15601 gccgatacca ttaaaggtca atgttccttt ttctcggatc actccgtgtt gcaaaatatg
    15661 cccgatcgta tgaggggctt tattggttac tcgtgtatcg attccttgga tttgtctgcc
    15721 cgagctgatt gccacgattt tgacttctgc gtgtgcacct tctcctgcta aatctgagtc
    15781 aaagtcagcg atcacatgac cgtcattcat gacaccaatt gcccagtcga cagaagcatc
    15841 tcgtaaaatg tgcccacggc gattcatata agaagtaata ttttctccta attgatctac
    15901 ggctgaatat ttgatttttg aaccagcttt agcgatgact tccacgatga tattacctga
    15961 tgattttgct acttgttctt tggttgtctg gaaacgttcc agataagaaa attcactatg
    16021 ttcatctgcc acgatcaaca catgcttgaa gaagtgctca tcggatgcgc catcttgaat
    16081 gaataaggac tcgagtggtt catcgatcac gacattttta ggcacataca ggaaaatacc
    16141 gctgttcata aaagctacat ggagtgcagt caattggtct tcgttcatgt ttactgcttt
    16201 tttcatataa tattcttgga ctagttctgg atattcgatc attgcagtga acaagtcggt
    16261 aaaaatgact cctttttctg ctaattccac aggcagttgt tcaaaaatcg tccatgaacc
    16321 ttgttggaca agcacaggat tgtctttcat tgcatcaaat gccggaatcg tgcctgtttc
    16381 actgatggtt tgtgtttctt tcacatcaaa taacgaccag cgatggaatt tcacccgatc
    16441 gatatgcggc aatggcagtt catctgcttt cgctagtgcg tcttgacgga aggttctcat
    16501 ccaatcaggt tcgcctttac ttaaagaaaa atcatttacc gcgtcaagat aatcgagcca
    16561 gtttttttct ttcattcttg ctccccctta cgcttcttct tctttatagt cgattcctaa
    16621 ttctttactg ataccagcgt agccttctgc ttccagacgt ttagctaggt cagcattccc
    16681 tgtcatgact actcggcctt ccatcatgat gtgaacgaca tcaggtgtga tatagttcaa
    16741 taatcgttga tagtgggtga tgatcaatgc accgaaattg tctccgcgca tttcatttac
    16801 tcctttagca acgactttca aagcatcgat atccagacct gaatcgattt cgtctaaaat
    16861 tgcaaatgta ggttctaaca tcaacaattg caggatctca ttccgtttct tttctccgcc
    16921 agaaaaacct tcgttcagat aacgttcagc catttcttct ggcatattta gtaaagccat
    16981 tttttcatct aactttttca aaaatgccat aacagaaatt ttgtcgtttt catcacgttt
    17041 cgcattgatc gctgcacgca tgaattccgc attcgtgata cctggtatct cacttggata
    17101 ttgcatagct aaaaataagc caagtcgcgc acgttcatct acttccaagt ctaaaacatt
    17161 tttcccgtca aacaaaattt cgccttctgt tacttcatag tttggattgc ccatgatagc
    17221 ggcagataag gtagattttc ctgtgccgtt aggtcccatg atggcatgga tttctccggt
    17281 tttcattgtt aggtttacgc ctttaaggat ttctttttcc tcgatcgaca catgtagatt
    17341 ttttatttct aagacagaca tgcattatac ccccaaattc tctttcgttc tcactcattt
    17401 tagactaatt gagaatgaag cgcaatgacc ttccttacta aaatcaaata tcttttattt
    17461 attattaaac aattttgatt tctaaattag aataggtata aaaaagatga gaaataagcc
    17521 attacagact tatttctcat cttttttgtt ccatacctta ttttgcatca gcctttactg
    17581 gtttaacaga gccgccccat ttgtctgtga tccattcttg gacttctttg cggtgaagca
    17641 cttcaatcag ttttttgatt ttgtcactgt cttcatcacc tttacgagtc gcaataatat
    17701 tcacgtaagg agagttgtcc ttttcaagca atacggcatc ttctaatgga ttcaaccctg
    17761 cgccgtaagc gaagtttgcg ttgattgcaa ctaggtcacc ttcatcattt tggtacgtag
    17821 aagctaaaag tgatgggtcg atcgtatggt tgaacttcaa gttcttaggg ttttcttcaa
    17881 tatcatcgaa tgttgctgtt tccagatcta ctccatcttt gactttgact aagccagcat
    17941 cttgtagaat tgtgatgatg cgtccccagt ctgattcaga atttgaagta atgattgttg
    18001 caccatcttt taattcggaa acatctttga cttttttaga gtacaagccc attggttcga
    18061 tgtggacagc accggcattg acaaagtcat atcctttttc tttgatttct ttgttcaaat
    18121 aaggaatatg ctggaagtaa ttggcatcaa tgtcgccttc ttccaaagat tggttcggta
    18181 atacatagtc atcgaatttg acgatttcaa gatcgattcc ttcatctttc aataatggtt
    18241 tcacatgttc caaaatctct gcgtgcggtg tcggtgaagc gccgactttc aatgtcgtgt
    18301 cgtctgcttt atttgctgaa tctttgctgt cgctgttgtt accgcatgct gctaatccta
    18361 cagtcagcaa aagcgctgct gcaaatccaa aaattttctt tttcatatcc tacatccccc
    18421 tatatcctat tatcttttgt ctgttttctt agttaagaaa tcaccgattc cttgaacgat
    18481 aaatacgatt agtaaaataa ttaaagtcgc aacgaatgtc actgttagat taccacgctg
    18541 gtagccttcc tgccaagcaa gacctccaag tccacctgcg ccgatcgcgc cggccatcgc
    18601 agtaaatccg atcatcgaga tcgttgtaac tgttaatcca gaaatcaatg cgggaatact
    18661 ttcaggtatc aagaccttcc agatgatttc ccagtaactt gctcccattg catctgctgc
    18721 ttccagtaca ccagggctga cttcacgaaa agcaatctct actagacggg cataaaatgg
    18781 tgctgcggat aaaacaagag ctggaatggc tgcttgcgca cctagcatag tgcctacgat
    18841 tgctttagta aatggaatga tcaaaaccat caggatcata aatggcgttg accggaaaat
    18901 attactgatg atcgatacga ttccataaat gattcgagca tacggtttgt ttttgcgtcc
    18961 aatagagtaa aggatcaatc ccaaaactaa tccgatgatc gtcactaaga ccattgagat
    19021 taatgtcata tatagtgtat caaggatagc ctgattcatc gtttccgggt tgatttgttt
    19081 aaaatctaga tatgtttctg aaaagctttt atccacgatg gatcacctca attcctactc
    19141 cttgttcttt gatgaacact tctgcttgat ctaattcttt ggtttcacca gtcatcaaga
    19201 cagtcaatga accaaaggaa ccttctttca cttgtttgat ctttccatac acaacgttga
    19261 catcgatgtt atagcgtcga atcgcttggg aaatgactgg ctcgttggca tttctttcgc
    19321 taaaggtcaa agttgccaaa cggccgcttg gattttcttt gatgagttca gctaatagtt
    19381 ctgtcgaatc agcttctggt tcgatgtctt gttgtacaaa gcgtttggtt acttcttttt
    19441 tcggttgtct gaagacagtc aatacgtctc cttcttctac gactttcccc aactccatca
    19501 cagcgacttt gttgcagatt ttgcggatca cattcatttc atgtgtgatc aagacgatgg
    19561 tcaaattcaa tttcttattg atcgctaaca ataagtctaa tacttcttct gttgtttgcg
    19621 ggtccaatgc gctagtagcc tcatcacaaa gcaggatctc aggatcattt gccaatgctc
    19681 gtgcgatacc gacgcgttgt ttctgtccgc cggaaagttg tgaaggatat gcttgtccgc
    19741 gtccttctaa cccgactaaa cgcaacagtt cttctgcccg ttctttacgt ttgctctttg
    19801 gtacgccagc taattccagc ggcagctgga tattttcaag tacagttctt gaccataaca
    19861 ggttgaaatg ctggaagatc ataccgattt tctttcggaa tgtacgtaat tctttgtttt
    19921 ttaattttgt aatatcttga tcgtttacga tcacttcgcc gctcgtggga agctcaagtc
    19981 cattcaggag acggactaat gtactttttc ctgcgccaga atagccgacg attccataaa
    20041 tatccccttt ttcaacgttt agtgaaacgt cattgacggc ttggatgttt ccgtttttcc
    20101 cattgaatgt ctttttgaca ttcgttagtt ggattaatga catgccattc acatctttct
    20161 cttatgattt ttcaaacaaa aaagccttcg ccctcttgcc ttaagcaaaa ggacgaaagc
    20221 ttgaacttcc gtgttaccac cttgatttac cgttacttcg caataacagc ctcttccagt
    20281 acttggactt agcttctttc cgtatactgt cgcgctgtaa cgggcactcc cgtagtaagt
    20341 ttgcttacgc tcgcttactc tgctctaaga ccatcttcca tctcattttc gttacccatt
    20401 ttcagcaaac agggctctct tcaaacgtcc gtgacatgta ctcttctttt caaagctttc
    20461 tcgtttcata taggattgct tatgattata gcaactggaa gaatcagtgt caactttttt
    20521 caagtgattt tctgatttta taattcgtca aattcttttg gatcaacatc tttgaaactt
    20581 aagatccacg cattcatttc atctgaatca ttcaagatat tgatatcttc accgatttta
    20641 tcattaacag aagaaactgt acccgttagc gggctggcaa attcattgac cgctttttcc
    20701 gcttcaactt cgatcagagg ttcatctttt tttagcgatt gtcctgcttt tggcagattg
    20761 gcaaacgtga tgtctccaag ttcttcttgc gcttcatttg tcaaaccaac acagtattct
    20821 tttccattaa ataagatcca aaggttgtcc tttttcttca aatgcttgcc tgccatttta
    20881 tatcagttct ccttcataaa ttttttcatt gaatccaatt gcaacaagct ggtcctctcg
    20941 taagatcagt ggtcgcttga tcagcatacc atctgtcgat aagacttggc tcgcttttga
    21001 agccgtaaac tcgcctactt gatctttcag tccaagttcc cgatatttca tcccgcttgt
    21061 gttgaacagg cgacggattg gtacttgact cgtttctagc caagtctcaa tcgttgaggc
    21121 cttcggtact tctttgatca tatcaacggt ttgataacta acgccatgct ggtctaacca
    21181 cgctttggct tttttgcatg ttgaacattt cgggtaccag taaaaggtgt acaattttct
    21241 tacctccttg aagatcttgc aggtgcttca ttttggtatc tcatcgataa tatcaacccg
    21301 ataccgatca tgttccctaa aatcgaggat cctccctgac taatgaaagg caatgggatc
    21361 cctgttaaag gaagtaatcc gatatttgct ccaatattct caaagacatg gaacagcagc
    21421 atcatgatca aaccgcttgc gatataggcg taaaattcat tattcgtatc gaaacacacc
    21481 cgaatcatac gatagattaa aataaaatac aagaaaatga cgaatgcacc accgataaag
    21541 ccgaagtttt caccgataac ggtaaaaatc atatcggact cacgaacagg cacatagata
    21601 ttgcttacgt tgaatccttt accaaacaag ccaccggtac caattgccaa aagtccttgg
    21661 gccggctgat aactcattcc tgacgtatca tggaaagggt ccagccatga atcaatacgt
    21721 gcaaactgat aagatttgaa tccaacttta taaagcaagt ctcttccgcc ttcagtcgta
    21781 accaagaaga tcgttcctgc tccaacgact actgccaaga ttactacagg tgcgatgatg
    21841 cgccaagaga ttccagacat caggaaaatc ccgccaaaga tcgcaagaaa gacaagcatc
    21901 gttccaaagt catcttgggc catgatcaat gctaaaaccg gtattgtcac gatcaagagc
    21961 ttcccaatca gccaaaagtc cgatttcatc gtccgttctt ttacttgcgt attatgttta
    22021 gttacgatca gtgccatcat caagatgtac gcaatcttca tcaactctgc tggctggaaa
    22081 gtaaacgcac cgaatcgaaa ccagttttta gaccctgttg aagcagctaa attctgatca
    22141 tagaatttga gcaagagtcc cattaccact aaccccaaac cataaaggta aggagtcagt
    22201 ttccagatcc atttggaatt caaatgcatg atgacaacga ttgctgccgc tccaactaaa
    22261 taccagacaa cttgcatccc tacgcctttc aagactgctg gattgttcgg atcatggcta
    22321 agtgctacat ataatgagag cagaccaatc aaacataaca gaaagactgg taaaatcacg
    22381 ccataatcaa tacgattatc gttcgaagct tttaattttt tttccatgtt tcatcccttt
    22441 tcttaaaaac catactgctg attattatag cggtccagtt agataatgga aagtctttcc
    22501 atagacattt gggaaaaatt aactttccgt ttgtgcagtt gcttgagcaa atgcagtaca
    22561 aaagcctact gcttatcttt agtgtatcat agaacttttt ttgatacatg caattatcta
    22621 ccagctgaga ttctcctcgc tgtgagaagt ttctgtgctt tcattgtatc gaaggatctc
    22681 ttcggcaatc tctagcagct gatcagaaac cagattggtt cgttggatga aataaaaata
    22741 tcgatggaaa ttcaacggag tcattggcac attttctgga acagagcgct tagacaaaat
    22801 cgagcgaccg attccttcct tcagcaacgc aacaattaca tcgttacttt tcacgatcat
    22861 cttcttatct tggatattca tctcttcaaa ataacgctgc gtgtaatgaa aaacaccaga
    22921 gtttgcttct cttacaagcc acaatttgct ttttgggtcc ccagcgatga ctagctggtc
    22981 ttccatcaaa gagtatcttt gtacgccgga agtctctaat ggcttttcga taaaaccaaa
    23041 atcggattca tgtttcgcta cttgttctaa tgattcatga gaattcgtga tttcgatctc
    23101 aaaatgcact ttaggaaacc ttttgatcag atgtttcatc aactgaggca aaagatagac
    23161 agcaaaggtg tgggaagcaa cgatccgaca tacttcttct tcattttgtt cattgagcaa
    23221 tttttgctga gtttcttccc attcttctaa caaaaccagt gtccgttcat ataataagtc
    23281 ggcttgctta gttggaagca tctcttttct tccgttacgt tgaaaaagat cgatattcaa
    23341 ttcccgctcc aactgtttga tttgattcga gactgctggt tgagataaaa acagtacttc
    23401 tgctgctctg gaaaaatttc ttgtttcata tactgctcga aaagtcgtta ataacttaaa
    23461 catggcacca cttccataac tttttatgat aattactata aaaactattt gatttaaata
    23521 tacaaaaaac catctataat agaaatgtac aacaaaacag ttgctgatcc aactagtaac
    23581 ttagatgaag acaatcagct gtctgcttgc tcgtaatcat acattcctat tcattttaca
    23641 atgtattcgt tcatcttggt actaaaatct attaaataaa aaggagaata ttatatatga
    23701 agaacttttt gaaaattatt ccgatcccga tctgcgggtt gatccttggg ttagcttccc
    23761 ttggaaatct gtttaaagac tatcaccatg ttgctttagg caatctaact ggcggtatct
    23821 ctatgattct tatgatattc atttttgtta aattgtttat cttattcgaa cacacgaaac
    23881 aaacgttgca tgatccaatc atcgcctccg tttcaccaac ttttacgatg tcactcatgg
    23941 ttatttgtac ttattttgtt tcatatgaaa gtattgcacc aattgtcaaa ttcatttggc
    24001 tgtgcgctgt tatcttccaa gtgatccttg tcttttattt caattatcac catgttatca
    24061 aagctgatct ttcaattgaa gcaatttatc caagctggtt tatcatttac gttggatttg
    24121 gggtcatcac tgtaactgcc gggaacttct tcccacttat tggaaaaatt ttcttttgga
    24181 tttcattgat ctgctatgcg ttcttattgc cgatcatcat caaacgtgtc tttttcgtaa
    24241 gaaatatggc tcatccgaca cgtccgctga ttacgatcgt tgcagcacct ggttctcttt
    24301 gcctaacagg ttatcttaaa aactttgctg aacctaattt ttatcttgtc gtagcattgt
    24361 tcgctttatc tcaagtattg tatttcgttg tgctgtctat gcttccacgt ttgttcaagc
    24421 ttcgttttta tccaagctat gcagccttta cattcccgct tgttatctct gcaacagcta
    24481 tatttactac tgttcattac ttcaacagtt taggtatcta tagtagcgta ttggatttct
    24541 taaaagttgc ggaatgtctg tttgcaagtg tagttgtctt ctacgttctt gctcattacg
    24601 tacgtttctt aattaaagaa catagcaaac atggaaaatt tgtttctaaa aaaatcgctt
    24661 aataatcgat tcaataggtt gtgaaaaagc tgttctgcat caagtaataa gaacggccaa
    24721 acaaaaacag ctgttcatgt ttttgtactg gttgggctta ttaccgcaga tgcaagcttt
    24781 tgaacaccgt ttaccaagag gtcgtaaaag aagtggtcag cttcaagaaa taagaaggaa
    24841 tttacgaaaa ttgcttctca aatttttgtg aaatttcggc ttatttccgt agaagctact
    24901 tctggaacac cgtttattcg aaacaagagg tcgtacaaga cttttgtcac gacctcttgt
    24961 tcttttgtca aaatactggt ttagtgctac tatgttcgag tatgctttta gtatttattg
    25021 acgaaaggaa ccgatcaaat gaaatcattg tcacatccaa taaaatctgt aaaggattca
    25081 gcggtccgcg aaatcttacc cggtttactg ctttcttttg ccatcgctgc cttcagcagg
    25141 cttcttgcta tttatctgcc acagttaggt ggagcgacta ttgctatttt attgggtatt
    25201 ttattaggaa atactttttt taaacagcct gttctagcaa tcggcacaaa atttgctgaa
    25261 agcaaactat tagaattctc tgttttttta ctaggtgcaa ctgtcacagc tcaaacgatt
    25321 gctcaaattg gtgtaaaagg aattgttttt gtgttactgc aaatgtcagt cactatcatc
    25381 ggtgcttact tgattggtaa aaaactgcaa ttcagcgata cttcttcttt gatgatggct
    25441 ggaggaaacg ctgtttgtgg ttcttctgct attgcttcaa tcgccccttc catccatgcg
    25501 aaagaggaag aaaaaggaca gatcatcaca ctagtcaatc tactgggtac tgtgatgatg
    25561 ttgactctcc cttttcttgg attacagttg tttggtggaa atctcattga aaaaagtgca
    25621 cttttgggtg gtacactcca atctgttggt caagtagtcg ctggggcaag cttacttgac
    25681 aattctgttg tcgaattcgc catgctattt aagattctac gtattatgat gctcgttgtt
    25741 gttgtctatc tgtttgaaaa attcatgcac aagaaacaac cagctctgtt tgaaacgcca
    25801 actgatacgc agaaaaaacg aaaattaccg cttccttggt atgtattagg gtttgtgatc
    25861 ttttgtttgg tcaacagctt gttttcttta cctgtagtta ttgaccaatc ggcacatttt
    25921 atcagcagct ggtttgagat cactgcttta gcggcaattg gtctgcgttt agactttgca
    25981 aaattcatga aagaaggtac acgcttctta tcttatggat tagctgtcgg gcttctccaa
    26041 acagtgatgg ctttactatt tatttggctg atcaaagttt agaatgaaca tgaaaaaaca
    26101 gttgtgattc tcgcatggaa caatgtccga atcacaactg tttttaattt tgcagatatc
    26161 cttctaacaa caacaattgg cgtttgattt caaccccatc tattgcgttt cccatatatc
    26221 ctgtcagttt tccattttgc ccgatcactc gatgacaagg aataacgatt ggcaatggat
    26281 tggttcgatt tgcctgccca atcgctcgca cagctttcgg actatcaaca gcaacggcta
    26341 cttctttata cgttcggatt tctccataag gaatggtcga caaagctttc cagacatttt
    26401 gctgaaaagg cgtacctctc tttatagata aaggaacatc aaattctttt ctttttcccg
    26461 caaaatattc ttcaaactgt tcttgtgtgc gcgttaaaat tcgctgattc gctgattcgc
    26521 tttctgaaat tggctgaaaa gatacagcag tcaatccgga ttcatctgca tctaaccata
    26581 aaaagccgat aggtgtgtgt attttcattt tgtttgctcc tagtcaatag actggaactt
    26641 cctccttttt ctctatccaa aactacttgc caactaataa aaaatgttct taaaagtttg
    26701 ataaaccgag gaaacatggt aaactaaata cgttagttta caaaaagaat acctgttctt
    26761 ttacgtaaaa tcaaatttta aaggtgttat ttaaggttta ttaaattttg aatgtaaacc
    26821 atatataaac atacgattga aaggatagaa acgatgaaag tatcaattgt tgtcccatgt
    26881 tttaatgaag aaaaaactgt tcctcttttt tttgatgagg ttgaaaaatt caaaggtgcc
    26941 catgagttcg aatatttgtt tattgacgac ggatcaaaag acggtacgtt agcagcaatc
    27001 aagaagctag cctcagctta tccttatgtc cgctttgtct cattttccag aaattttggg
    27061 aaagaagcgg ctctgtacgc tggattgcaa gcttctacag gagatttagt gaccgtgatg
    27121 gatgttgatc tgcaagatcc gcccgaactg cttcctgaga tgatcaaccg gatcactact
    27181 tctgatatcg attgtgtcgg cacccggcgt tcgactcgtg aaggcgagcc tgcgatacgc
    27241 agtttctttg cgaaaaaatt ctaccagttg atcaataaaa tcagcgatac agaaatggtc
    27301 gatggcgctc gtgatttccg cttgatgact cgtcaaatgg tggatgccgt cctagaatta
    27361 acagaataca accgattttc aaaaggatta tttagctggg tcggatttaa aacagaatat
    27421 atttcttatg aaaaccgcga acgtgtggct ggcgaaacat cttggtcctt ctggaaatta
    27481 ttcaactact cgattgatgg tatcgtgaac ttctcggatg caccgttaac gattgcttca
    27541 tttatcggcg ctttgtcttg tgtcggttct ggtattgcaa ttttgttcat tattttgcgg
    27601 gcattgttgt ttggtgatcc tacatcagga tggccttcca tggtctcgat cttcctgttc
    27661 attggtggaa tccaattgtt atgtcttgga atcatcggga aatatattgg gaaaatcttc
    27721 ttagaaacga aacagcgtcc tgtttatatc ataagagaaa cagaaaaagt gaagccaaat
    27781 gaattgaagc ctcaccaata atatacgtca aatgaagtaa acattttttt aaatgaaaaa
    27841 ggactgtgac aaaagtcttg tcacagtcct tttttccgaa taaatggtgt tccagaagta
    27901 gcttcttcgg aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
    27961 ttcttatttc ttgaagctga ccacttctat cacaacctct ttcatatctt attacttctt
    28021 cattgctgct tttagtgctg ctgctaatgg gctttcttct ggttcttctg cttgtgcata
    28081 ttttttcagc aagcgtcgtt cttcatgttt ggtcattttc ttcttctgct cttttttatc
    28141 aagcaatttt tctgtcgttc catcgtattt acaacggaaa taagcaccgt ttttaccttc
    28201 gatgatttcc attttccggt gacattgcgg gcatcgatga ttagagactt ttggatcttt
    28261 tcttctgcgg tagttgcaat ctgaattggt gcagacatag attttgccgt cacgtgtgtt
    28321 tttctcgcgc agattggccc cacattctgg gcatttcttt tgggtaatcg agaaatcttg
    28381 atattcttgt ttgcttgatt tgatctcatt caccaagcgt tttgtttctt tttcgattcc
    28441 attcaagaag gttcctgctt gtttctttcc tgcagcaata gcttcaaggt ctttttccca
    28501 gctttctgtc aaatctggtg ttactaaaga gggatttacc aatactaata attgcttacc
    28561 tttaggagat acttgcagtc cttgaggggt acgttccatc agttcagatt tgattagttt
    28621 ctcgataatc tctgcacgcg tagctggggt tcccagactg tatttttcca tttgaccaag
    28681 caagcttgct tctgacaatg gtttaggcgg tgcagatagt tcttttttga tggcgaaatc
    28741 gggattgatt ctcattcctt tttcccattt gactgtttcc gtctcagttt cagtttcgcc
    28801 tttccatcct gcttggacga cacgattttg ccgaaaaatg aatcgttctt taccaaattc
    28861 aaccgtaact tttgtttggc tgactttatg gggttgagca aacagtccta aaaaacgaga
    28921 gacaatcatt tgatagattt ttcgctcttc attacttaat ttttcataac gtgctctgtt
    28981 ttcagttggc aaaagtgcat ggtgatcggt cactttactg tcttggaata cttttgtctg
    29041 ttggacgatg gctccgtttt tcaaatactg cttgacctca ggagcaaagt cgctgactgc
    29101 ttgcaatcgt tctttcatcg tacttttcat gtcatttgtc agatatttgc tgtctgtacg
    29161 cggataagta acgatcttat gggtctcata caaactttgt acaagactta atgttttctt
    29221 tgcagaatag ccatagcgtt gattggcttc tctttggatc tctgttaaat cataaggcaa
    29281 tggcgcagat tccgttttta atttttcttg gacttctgtg acagttcctt tgcttgtact
    29341 gatcaattga acaagttttt gtgcttcttc ttgtgttttt aaagcaaatt gatttttttg
    29401 aagcattttg gcttgttctt ttccagcttg caacgtgatc gtgaaatacg tttgtggacg
    29461 gaactgctcg attttctttt cttggtctct cactaatgct agtgtcggtg tctgcacacg
    29521 gcctgctgat aaattatccg cgtacttcac agttaaggct ctggtcacat tcaatcctac
    29581 cagccagtcc gcttttgccc gggcaagagc agattcatac aatgcatcat attctttcgc
    29641 aggtcttagt tgcttgaatc cagttttgat tgctttatct gtttgagaag agatccataa
    29701 ccgcttcact ggtttattga aatgcacata ctctaagatc cagcgagcaa caagttcccc
    29761 ttctcttccg gcatctgtcg caatgactgc ttctgagaca tcttttcgct gagccagctg
    29821 cttgattgct tttaattgat gtcctgtttt aggaagtggt ttgattccta gttttttagg
    29881 gatcattggc aacgtgtcca tttgccagct ctgccattcc ttattcagat cttccggcat
    29941 tttcaatcct aataaatgac caagggccca agtaacgatc accttgggtc cttcataata
    30001 atttttgtgc ttttgattag caccaagcac cttgcttagg tctttcgcaa cgcttggttt
    30061 ttccgctaga attagttgtt tcataaaatt cctttctgct ttctactgtg atgggctgat
    30121 gatttttcaa caacgcaagt cccttgtatt gctgtaaatc atcgtcacac tcttcacacc
    30181 agcgctcatc tccgtcattc caaaaggcgc tgagaaagtt actttttgca ttgatatatt
    30241 cataattttg atggatttcg ttccattgtt tttcgtacgc ttgtcgtgct tcttcgaaag
    30301 actcgaatgt ttcttcttct tcaatatctt cttcccagtc ttcaaaaaac caccaaggct
    30361 cattgtcccc gaacatggta attacttgat acatactcgt gctccttctt tcagcttttc
    30421 tagactcatc ttagtcactt ttcaagaaag ttgcaagaat tttctcttat aaaagggtca
    30481 tttaatgaac atcttaaaat tgttacaaat gaatctgatt gtttaaggca aagtagcgat
    30541 aaagttattg atcatccaaa aatagctgtc ttctggatcg attgttggtt cttttgtaaa
    30601 gtatcctaaa aaacgtaagg aaagataacc gtggatcata ctacgaagtt ccctgcttcg
    30661 atgaactttt tcttctttcg ttaattgaaa agcctcaaat atctgcaaaa tgatctgatt
    30721 cgtttcatgg attccatcta ataaaacagc ttcattggta tgcggtacac tgatcagtaa
    30781 ctcatacact gcttgatttg aaaaagcaaa ggatcgataa gtttctgcat agatctgcag
    30841 tgcttcctga ccactttttc ccacaagagc ccgtcgcagc tggtcattaa gttcctgcag
    30901 aagtttcgct gttagcgctg ttttgacatc acgaatattt ttgaaatggt tatacagcga
    30961 gggagccttg atatccaagc gtttggctaa atgctgaaag gttatttgct gaacatccat
    31021 ctcttctgct agttctcgaa agcagtcgat gattttctct tgagacagat ttcttttttt
    31081 catttccatg tttcctctca taaaataaat ccttcactct ttcagtttac cacgaaaaag
    31141 aaagagaaaa aagagacttt cattgataaa ctacatattg tagtttataa taaagagctg
    31201 tagatgaaaa ttttcagaaa gaaggagtct aattggctgc aagaataaag gaaaaattat
    31261 ttaggaaaag agaaacttgg gaacaaaact tgtttgtctt atggtttgga acatttatgg
    31321 ctggtatcgg atttagttta gtcatgcctt ttatgtcact ttacatagat acgttaggta
    31381 attatagtac gtcacaattg aacttttgga gcggattgac tttttcatct actttcttgg
    31441 tgactacgct tatctctcct tggtggggaa aattagccga ccaaaaagga agaaaattga
    31501 tgctgttgcg tgcttcatta ggaatggcaa tcgtcatttc tttgatgggt tgcgtgacta
    31561 gtgtgtatca attagtcgca ttgcgtctgc ttcaagggat tttttctggt tatatcagta
    31621 atgccactgc ccttgtcgca actgggacgc caaaagaaaa aagtggtcaa gttcttggaa
    31681 ccttagcaac tggttctgtt acaggaacat tacttggacc gcttcttggc gggatctctg
    31741 cttcagcgtt tggctatcgc cctacctttt tcattacagg gatcatctta tttttcgtct
    31801 tcttgttgag tctgtttttc gtccatgaaa aatttgtccc agtgaaaaaa gaaaacatgg
    31861 tttctgccaa acagattttc aaagacttga aatatccaca cgtcgtcatc ggtatgttcg
    31921 tcacaacgat gatcatccaa gcatccaaca attccattag tccaatcatc agtctgtaca
    31981 ttcgccaatt gctgcatgga catgggaatg ttacgttgat cagcggaatc attgcttcga
    32041 tacctgggat tgccactttg atagccgctc caagatttgg tcgcttaggt gacaagattg
    32101 ggagtgaaag aatacttgcg attggtttgg gttttgcgat tctggtttat atcccaatgg
    32161 cattcgtcac gaatgtttgg caattagctg ccttacgttt tttgattggt atctctgatg
    32221 cttgtctgct ccctgctgtc caagcattga tcacaaaata ttcgcctcat caagctgctg
    32281 gacggatctt cagttataac caatctttcc aagcaactgg taacgtagta gggcctatga
    32341 tcggctccag tgtttccagt gcatttggtt atcgtggcgt ctttctttcg acttctcttc
    32401 ttgttttggt caacttcttg cttgtacgac acaatacaca agagatccat cagcaagaaa
    32461 ctgcagaaga aaaagcttca ggagtctcca tagcttcagg gcactctcct tctcatagac
    32521 aataagcgta aatgaaaaac actgcttgaa cagttgtcac agactgtcct tgcagtgttt
    32581 ttgtttaggc ctgttttttc aagaactcag ataagaggaa taatcccttt tccaaggttt
    32641 cttttttaca acagtaacct agtcgggcat gcttcggtaa atcaaaggca cttcctggta
    32701 ctaatagtac tcctgtttct tctaataaat tgatacaaaa tgtctggtca tcttcttcga
    32761 tggctaattt aatgaaagaa gtagagacgt aattgggagc gacaagttca actttcggtt
    32821 cattggctac ccattgtttt aaaatcacaa ggttctctgt aatgatcttt ttgtttcgcg
    32881 ccaatatttt ttctttattc ttcaatgcat gtacagcaag atcatctgac aggactccgc
    32941 cgcaaatcat cgtgtagtct cgatactttc gaaaaacttc tgccaactca ggtccagtag
    33001 ctgtccagcc aatacgaata cctggaatgg agtaggtttt ggatagggaa ttggttgcta
    33061 tacctttttc atagacatct acgatcgaca agaattcacc tttatccgtt aaaggcgcat
    33121 atacttcatc tatcaataca tatgcatcta ctgtacgagc aatttctgcg attttttcta
    33181 aagttttccg atctagcaat gtgcctgtag gattgtttgc actattcaaa cagatgattt
    33241 ttgtttcagg tttgactaag gcttctagct gttctaaatc aaactgccaa tcttcttctt
    33301 ccttcaaatg aacaaagtca acagcggcac ctaaagattt ggggatgtca tataattgct
    33361 gataagtagg aagcatcgaa acaacatggt ctcctgcttc cactaacgcg tatagagcta
    33421 aaagattagc accagtcgct ccattcgttt gtaagatgtt ttccggatcg acggtttgat
    33481 aaagagaagc gacttcttgt ttgaaaacat ctgatccttc gatccagcca tagtccaatg
    33541 gttcgttttt gcgtttttcg aaaaatttac ttacgctagt cccatctaag ccaacgagtt
    33601 cgtctaatgt caatgcttcg atcgagcttt gtgcaatatc atataccgct ttcttttccc
    33661 atttatttag ccattcttct acaccgaaat ttgcgatttt catgtttctt tcccccttat
    33721 atcttgatcc agcctacagc aagcaacccg attgaaagca gtgcggccac acaaatcagc
    33781 ccaatcatta ctttttcttt tcccgtaatt tgatgtttgg tttcacgaca agcgatccag
    33841 tagaagcaaa aaccaggaag ataggcaata ctacataaaa ggacataaga aaatcctgct
    33901 aaaatgatgc ccatcaattc aaaaactaca gcaatcccac cgatcactac ttgttttttg
    33961 attccttctt ttaaagaaaa ctggacttga tagatcccga caagtagata acaaatcaaa
    34021 attgccgctg tacataaaga ataggcaaat tgataggcat aatcagtaac taagaaagtc
    34081 aataagaata gattagtcaa gattgcagta acaactaacg aataagttgg ggcatttttt
    34141 ttattcgttt ttccccaacc tttcggcaac agaccgtctc gagccatcaa catcgttgtt
    34201 tctgctggga gcatcgtcca agacaaccaa gaaccaatga tcgaaataat caagccgata
    34261 ttgatcagcg ctgctcccca atttcccacg attcctttta aaatatttgc catagcaggt
    34321 tgtgaaatag ttgctaactc ttcttgagat aagacgccgt atggcaagat cgaagcaagg
    34381 atatagataa acaataaaca aagcagacca atgatcgatg cttgctgggc ttctgaacgt
    34441 ttcttcgcgc ggttggctaa aacacttgcg ccttcgatcc ctacaaagac ccacatcata
    34501 accatcaaac aacctttaat ctgcaaccag atcgttcctt gctgccctgt tccattcact
    34561 agattatcag atacgttacc ccaaaaatca gcagtgaata tattcacttt aaatgcgata
    34621 aatacaaata ttaaaaatag gaataatggc actaatttac agatcgtcac gattgtattg
    34681 atgacgctcg ctccttcaac tccattgttt actaataaag tcagacccca aataaaaata
    34741 cttgcaaata aaatagaagg aacattttgt ccacctttga atatcgggaa aaattctccc
    34801 atggcgctca tcagcatcgt tgcaaaagca acatttccca gccacgcaga caaccagtaa
    34861 ccccaaccac tgatgaagcc acctaattta ccaaatgaag cttccgcgta accgaaaata
    34921 ccaccatcta gatctggacg cttttctcct aaatgattca gcgataatac caaagctaaa
    34981 ataccaatcc caacgattat ccatgaaagg atcgctggcc ctggagctgc agaactagcc
    35041 agatcacttg tgatcccaaa cacaccgctt ccgattgacg aactgacaac cagagcaacc
    35101 agtccccatt tgctaatacc cgtcgatttc ttttccattt ccatcaccct cacttataaa
    35161 attgaacaaa tcacttgcaa aagattccct tctattttgc aactgctctt gcttaacaaa
    35221 aagcaacttt attcatatta ttggatatta tgaacttttt acacaagtat ttcaagtata
    35281 aaatgaataa aaattaattt tattgcaaaa aaacagctat gctatccaaa agaaaaggtg
    35341 tttcttttgt tagccatagc tgttttgatt catttaatat cgcttaaacg tattgtaaaa
    35401 cttttgtttc ttgatatgcg tggtcgatca aaccaccacc cagacattcc attccatcat
    35461 aaaatacaac tgcttgtcca ggagtgattg cacgtacagg ttcgtcgaat acgacttctg
    35521 cacgattatc atccaataag cgaactgtca caggaacgtc ttgctgacga taacggaatt
    35581 tcgctgtaca cttgaattct tttggcattg gttcattcgt cgtaaagtgg atttcacttg
    35641 catccaagct tgtggcatac aatgcaggat gatggaaccc ttgccccaca tataatgtgt
    35701 ttgtcgctag atcttttcct acaacaaacc agggttcttg cgaatctcct ccgccaccga
    35761 tccctagtcc ttgacgttga ccaatcgtat aatacatcaa gccagcatgt tgtcctttga
    35821 cttcaccatc aagagtgacc atatttcctt tttttgctgg cagatagttg ctcaaaaatt
    35881 gtttgaagtt cttttcacca ataaagcaga ttcccgttga atcttttttc ttagcagtcg
    35941 caaggcctgc gcgttcagca attgcccgta cttctgattt ttccatgccg cctagaggga
    36001 acatcgtttt tgaaagctgt tcttgtgata attggctaag gaagtaagtt tgatctttat
    36061 tattgtctac tccacgaagc atgtggacca cgccgttttc atcgcgagtg acttgcgcat
    36121 aatgtccagt agctacataa tcggcgccaa gatccattgc ataatccaag aatgctttga
    36181 atttgatttc tttgttgcac atcacgtctg ggttaggtgt acgtcctgcg cggtattcgg
    36241 ctaggaaata ttcaaataca cgatcccaat attctttttc aaaattcaca gagtaataag
    36301 gaatcccgat ttgagcagct actttcgcta catccttata atcttccgtg gccgtgcata
    36361 caccgttttc gtccgtgtca tcccaatttt tcatgaatat tccgatcacg tcgtatcctt
    36421 gttcctttaa caaaagtgct gttacagatg agtcgacacc gccgctcatc cctacgacaa
    36481 cgcgtgtttt gctgttgtct gtcatgatat caccatcatt tcaattagat tctgaaacat
    36541 ctacgagttg aaggtcgcaa cttttcagta ttttacatta tacgtcatat ttctgaaaat
    36601 ttcatccctt aattatcaag tcgtaaaaat actgttctac tggacctatt cagtacacaa
    36661 acatgatagt caaccaaaaa atcactcatc ttttgagcat tcgatataaa aaattggcat
    36721 actcataaaa agggccagcc tttcggcaat aaggaataat tttataaatt gtgaaaaact
    36781 atttttcaaa attgcttctt atcgcttaaa gctgcacgtc tcatttacaa ccttttcttt
    36841 ttagatgtta tgagttgctc agataagtga ttttgtttac tctaagaaaa aagggaggca
    36901 aaagatacat gaattacttt atcagtgata tgcacttttt ccatgaacgc ttattaggaa
    36961 aaaacgactt tgctccgcgg ctctttgcaa atgtggatga aatgaaccaa cagttgatca
    37021 ctagctggaa tgcagtaata aaagaaaccg accatgttta tcatctaggc gaccttgcta
    37081 tgcatccgca atatgaaaaa gcaaatgcag aaattttgga actggtcact caactgcaag
    37141 gaacaatcca cttgatcaaa gggaaccatg acagtcgcgc ttttttccgt tatttagaaa
    37201 aaaatgatcc taggcttact gacggaagtc ccaaatttta tttttatgat gtaggtgcca
    37261 tcgttaaatt cgaccatcaa caatattact tgactcatta tccgatgttg ctcgggccta
    37321 acgacactat ccgcaatctt catggacata tccatcatta cagcgtacct atcgagaacg
    37381 atgtcaatgt aggcgtagac gcaccggaaa gagaactctt aaaggaaaaa ctaccatttg
    37441 gcactccgtt atctgagcaa caaatcaaag aaatctatga agcaaagagg aagtacgata
    37501 ggtcgtgaaa aaactgccct atatcaagta ttaaacaaat aaagataatt actcatattt
    37561 ttcatttgag taggcttatt gccacaggaa cattaggatc agaaatggag ttgtaacaag
    37621 acttctgtca caactccatt ccatactatt cgtctttttg ctcatggtgt tcttttacca
    37681 gttttttctg taattcttta tcataatttc gggcagctga cgttaaaaat aaaacgattc
    37741 cgcataataa aatcccgatt ccggaaaaca agaacatttt ttctacccct aaacgatcag
    37801 ctaatggtcc tgcaaataat aatccaattg gacctgtcag gttcatcaaa gagttgaagt
    37861 tccctaagac acgacctaaa acattcgatt cataactctg ctggatcatt gccatcagca
    37921 gtgtattgaa atatggcgtt gcacaacctg ctcctgcatt caagataaga aagtacaaga
    37981 acccttgact atcgtttgga accaatccgc ttgcaccgat cgtcaggccc ataatgatgt
    38041 aggctataat aataggtttc attcggtctt tccatgttcc tataaaactg atcagtgcac
    38101 cgcctaagag cattccgact gcgtagacta cttccactgc tcccgcctgt ccaacggaac
    38161 caccaaagta atctagcgtc atcaatggat acagactgat tgctggcatg aacaacagca
    38221 tcacaaatgc accattgatc gttatgtacc ataacccttt attatccatc aattgctgca
    38281 gcccaaattt tgcatcttta aaaatagtaa gcggaacttc caatatttca ggagaaactt
    38341 ttgggatctt cacaaaaatc aataagccga caccaaaaac tgcccccaat acatctaata
    38401 aaataaggta attgacagga acgactgaaa acaaagccgc acctagagct ggcgcaatga
    38461 tgaaattcgc tgattggacc attcctaact gtccattcgt ccttgttatg tgagaagatg
    38521 gaacgattgt cggcataatc gactggatcg ttggcatttg gaatgtttgt gcgaccgaac
    38581 gcataaacaa agacacaaaa actagccaaa tcgggaagga ttctgaaatc gttccgacta
    38641 ctgctaaaat aagcgcgaaa atcgctacaa taatatccgt gacaatcaaa agagcttttt
    38701 tatcccagcg atccactagc ggtcctacaa atgggcttag taaaaccatg ggaatcattc
    38761 ctagtaaagt agcaaaactc aaaattgtcg ccgaaccggt ttctcttgtc aaataccaga
    38821 tgatcgcata ttggacgacc atactagtga ttcctgacaa aaactgtccc gataaaaaga
    38881 acaaataatt tctcttccaa ttcgttaaaa cgaccgactg ttcattttcc ataaacatcc
    38941 ctcttttctt tttcattttg tatagcgttt gcacaacaat cattatacgg gattcttttt
    39001 tcaaaaaata cctttttccc taaataacaa tcgtttcaat ttaaatttct ttacttagaa
    39061 acaataaatc agcaaaatat taaaaataac aacatgtgtt aaatttaaaa ataaaaaaga
    39121 caactaattt gcgtcgtcct tacaaagtta gtcatctaac aataggccgt ttcaaaacta
    39181 gttgtctttt tatatccaca gatcattcga ctttttcgaa taacccacgg ctaatcatcc
    39241 catccatcag aaaataaaat ttatcttggc tcattttctg ttttggatct ttgaaaatat
    39301 tgactgcttt caatccattt gctgtagtga tcgacatttt caatgtttga atatcttttg
    39361 atacagtgat ttttaatttg aatccttctt tgctttgcgg cgtggcatct aatggtcgga
    39421 tcaattgata attgcctccg tttgtttctg taaatccatt atcgcgcaat gtatatcttt
    39481 ttgcatcagg atgcaaacga tagctaattg gtgcgcccag taccgttgct gttgtttcaa
    39541 atgccatgat tttcacctcg ataaaattag tttctcttta ttataaaagt ttatcctgat
    39601 ttttactagg tttttcagtt aagttcccta aaaaatgaag gcgttctctc agacatctgt
    39661 tcatataaga atgacttcac tagttatttt ttgacagcac gaactaattg atcaaggaaa
    39721 tactggatgt cgtcttcggt atttcccaaa ccaaagctga tacggatgga ttcttttagt
    39781 atcggtgtat cttctccata cattgcttcc aatacgtgtg aaggctcgat atttcctgct
    39841 gtacaagctg aaccagtaga aatagcgacc ccattaagat caagcttcat cagtagcaaa
    39901 tcatttgctt cgccaattaa gcgaagattc aacacatgag ggagtttatt cgttaaatca
    39961 ccatttactt gataagggat ttcgtttgct tctaatgtat caagaatcaa ttgctggaat
    40021 cctttatatt tgttgttacg tgcttcacgt tcttcaggtg acagcagttc aactgctttt
    40081 gccatacccc agatccctgc taaattttct gtacctgcac gccgcttttc ttcctgctcc
    40141 ccgcctaaaa gcaatgcagg gatatttact ccgtctcgtt tatataagaa accgacgcct
    40201 tttggcccat tgattttatg agcagagata cttaataaat cgattcctaa ttcttctgga
    40261 tggatcgttt ggtttccgta cgcttgtacc gcatctgtat ggaaataagc tggatgatcc
    40321 gctaaaagtt caccgatttc tttgattggc atcaaatttc cgatttcatt gttcccgtac
    40381 atcacagaga ccaagatcgt atcttctcgt aacgcttttt tgacctcttc gatcgacaga
    40441 ttcccttttt catctacagg cagatatgta acgtcaaacc cctttgtctc taaatatttc
    40501 atggtattaa gaacagcagg atgttcgata gctgtagtga ttagatgtct gccttctttt
    40561 tgacgcgaga acgctgtttc taagatagct gtattatccc cttctgttcc cccactgtta
    40621 aagatgatct cgtgaggttt tgcttgcaga ctgtctgcaa tgatttgtct tgctgtttcc
    40681 aatttttcat gcgcgtggcg accaaatcca tgtatgcttg aaggatttcc aaacgtgtct
    40741 tgcatgattg ccgtcatcgc ttcaattact tgtggatgca tgggggtagt tgccgcatga
    40801 tccagataaa tagttttcac ttcattcatc ccttccaaat tattgctagg cacattctag
    40861 cataaaatag cgaacaaacc aaagaagagc tcatttttgt ctagaggacg tttcatgggc
    40921 atagaaaaaa ggagctgtga caaaagtctt gtcgcagctc cttgaagctg ctcacttctg
    40981 tcacaacttc tt
//
LOCUS       DAJSBM010000018        39803 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJSBM010000018 DAJSBM010000000
VERSION     DAJSBM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.018"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..290)
                     /locus_tag="ONT67_001145"
     CDS             complement(<1..290)
                     /locus_tag="ONT67_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2354900.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
     gene            complement(283..624)
                     /locus_tag="ONT67_001146"
     CDS             complement(283..624)
                     /locus_tag="ONT67_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354901.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            complement(798..1523)
                     /locus_tag="ONT67_001147"
     CDS             complement(798..1523)
                     /locus_tag="ONT67_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2354902.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            1576..2052
                     /locus_tag="ONT67_001148"
     CDS             1576..2052
                     /locus_tag="ONT67_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354903.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(2058..2240)
                     /locus_tag="ONT67_001149"
     CDS             complement(2058..2240)
                     /locus_tag="ONT67_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354904.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(2240..2539)
                     /locus_tag="ONT67_001150"
     CDS             complement(2240..2539)
                     /locus_tag="ONT67_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354905.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            complement(2644..2847)
                     /locus_tag="ONT67_001151"
     CDS             complement(2644..2847)
                     /locus_tag="ONT67_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354906.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(2862..3608)
                     /locus_tag="ONT67_001152"
     CDS             complement(2862..3608)
                     /locus_tag="ONT67_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCR2354907.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            3686..3994
                     /locus_tag="ONT67_001153"
     CDS             3686..3994
                     /locus_tag="ONT67_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354908.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(3965..4222)
                     /locus_tag="ONT67_001154"
     CDS             complement(3965..4222)
                     /locus_tag="ONT67_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR2354909.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(4520..4660)
                     /locus_tag="ONT67_001155"
     CDS             complement(4520..4660)
                     /locus_tag="ONT67_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354910.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            4960..5280
                     /locus_tag="ONT67_001156"
     CDS             4960..5280
                     /locus_tag="ONT67_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354911.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            5298..5729
                     /locus_tag="ONT67_001157"
     CDS             5298..5729
                     /locus_tag="ONT67_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2354912.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            5856..6440
                     /locus_tag="ONT67_001158"
     CDS             5856..6440
                     /locus_tag="ONT67_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2354913.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            6542..7690
                     /locus_tag="ONT67_001159"
     CDS             6542..7690
                     /locus_tag="ONT67_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2354914.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT67_001160"
     CDS             complement(7779..9404)
                     /gene="groL"
                     /locus_tag="ONT67_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR2354915.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT67_001161"
     CDS             complement(9455..9739)
                     /gene="groES"
                     /locus_tag="ONT67_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR2354916.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            9964..10626
                     /locus_tag="ONT67_001162"
     CDS             9964..10626
                     /locus_tag="ONT67_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2354917.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(10702..11994)
                     /locus_tag="ONT67_001163"
     CDS             complement(10702..11994)
                     /locus_tag="ONT67_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2354918.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12164..12793
                     /locus_tag="ONT67_001164"
     CDS             12164..12793
                     /locus_tag="ONT67_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR2354919.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(12896..13705)
                     /locus_tag="ONT67_001165"
     CDS             complement(12896..13705)
                     /locus_tag="ONT67_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2354920.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13760..14629)
                     /locus_tag="ONT67_001166"
     CDS             complement(13760..14629)
                     /locus_tag="ONT67_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCR2354921.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(14630..15946)
                     /locus_tag="ONT67_001167"
     CDS             complement(14630..15946)
                     /locus_tag="ONT67_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354922.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT67_001168"
     CDS             complement(15943..17778)
                     /gene="walK"
                     /locus_tag="ONT67_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCR2354923.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(17783..18487)
                     /locus_tag="ONT67_001169"
     CDS             complement(17783..18487)
                     /locus_tag="ONT67_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2354924.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(18677..19537)
                     /locus_tag="ONT67_001170"
     CDS             complement(18677..19537)
                     /locus_tag="ONT67_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2354925.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(19524..20093)
                     /locus_tag="ONT67_001171"
     CDS             complement(19524..20093)
                     /locus_tag="ONT67_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCR2354926.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            complement(20667..21836)
                     /locus_tag="ONT67_001172"
     CDS             complement(20667..21836)
                     /locus_tag="ONT67_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2354927.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            complement(21880..22110)
                     /locus_tag="ONT67_001173"
     CDS             complement(21880..22110)
                     /locus_tag="ONT67_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354928.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            22186..22258
                     /locus_tag="ONT67_001174"
     tRNA            22186..22258
                     /locus_tag="ONT67_001174"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22219..22221,aa:Lys,seq:ctt)
     gene            22420..22641
                     /locus_tag="ONT67_001175"
     CDS             22420..22641
                     /locus_tag="ONT67_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354929.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(22965..23210)
                     /locus_tag="ONT67_001176"
     CDS             complement(22965..23210)
                     /locus_tag="ONT67_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354930.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(23204..23605)
                     /locus_tag="ONT67_001177"
     CDS             complement(23204..23605)
                     /locus_tag="ONT67_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2354931.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT67_001178"
     CDS             complement(23777..25585)
                     /gene="uidA"
                     /locus_tag="ONT67_001178"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR2354932.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(25613..27379)
                     /locus_tag="ONT67_001179"
     CDS             complement(25613..27379)
                     /locus_tag="ONT67_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HCR2354933.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(27392..28186)
                     /locus_tag="ONT67_001180"
     CDS             complement(27392..28186)
                     /locus_tag="ONT67_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2354934.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(28197..29573)
                     /locus_tag="ONT67_001181"
     CDS             complement(28197..29573)
                     /locus_tag="ONT67_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2354935.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(29702..30349)
                     /locus_tag="ONT67_001182"
     CDS             complement(29702..30349)
                     /locus_tag="ONT67_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR2354936.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT67_001183"
     CDS             complement(30433..31860)
                     /gene="uxaC"
                     /locus_tag="ONT67_001183"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2354937.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT67_001184"
     CDS             complement(31934..33013)
                     /gene="uxuA"
                     /locus_tag="ONT67_001184"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2354938.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(33016..34647)
                     /locus_tag="ONT67_001185"
     CDS             complement(33016..34647)
                     /locus_tag="ONT67_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2354939.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            34964..35635
                     /locus_tag="ONT67_001186"
     CDS             34964..35635
                     /locus_tag="ONT67_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2354940.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(36471..37427)
                     /locus_tag="ONT67_001187"
     CDS             complement(36471..37427)
                     /locus_tag="ONT67_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354941.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(37536..37781)
                     /locus_tag="ONT67_001188"
                     /pseudo
     CDS             complement(37536..37781)
                     /locus_tag="ONT67_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37926..38348)
                     /locus_tag="ONT67_001189"
     CDS             complement(37926..38348)
                     /locus_tag="ONT67_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354942.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(38528..38899)
                     /locus_tag="ONT67_001190"
     CDS             complement(38528..38899)
                     /locus_tag="ONT67_001190"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2354943.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(39233..39496)
                     /locus_tag="ONT67_001191"
     CDS             complement(39233..39496)
                     /locus_tag="ONT67_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354944.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT        12077 a         7912 c         6098 g        13716 t
ORIGIN      
        1 tctgtttccg tatgtccttt aaccgttact gagacagata cataagtccc tgtttcatcc
       61 ataagaaaag gtttatattc ctcaacaaga aagtcttgat gagttccaga aacaaccctg
      121 tagtgtttat actcattaat agttaccgtt gcctgtggat cattcttttt cataatctcc
      181 cacgcgtgag cccaagataa ataatcaaaa tttccttttt tcttgagaat tttatttaac
      241 ttacgactaa aaagtttttc aaagttcgtt gtccctttga tttcactcat caaattctgc
      301 ctccatttca gcaatgtatt tcttacctga tccgtaataa gagatatcaa tcaagttatc
      361 tctgttgtac tcttctagcg catcaatcaa gccatcttcg atgacataga tgtattcagg
      421 tttatttgaa tgttttgata gatggataag gtaaacatga tcccaaatac tcacaaaatt
      481 tcccaagtca tcttgatcac atgatagttc ttcgttcgtc aaaagatttc gtctgatttt
      541 tcgattattt gtttccttga tattcgactt gccccattca gaatcagtca aatattgatc
      601 taaagtggaa agttctttat tcatatgcta aaattctcct tagatatgtt ttgtgactct
      661 atgcttgccg gctgagtcac ttttttattt gttgccatgc tttttgcttt tcgatatgtt
      721 gttggcttag gatgtttggt ttattgtgtc tccaccagcg attagcaatt accgtcccta
      781 ttcttagcgc ttcagctcta ttcattttca tcaccgaaaa gtctttgttg tctgttcagt
      841 tgatcgattt ccatgcggat cgcagtttct ggtaaccaca tttcaataaa tgaaacagca
      901 tcatcgaatc tcttacgagg taactcgcca tatcttggga ttgaaaaggt acgtttaaat
      961 tcagaccaaa attttgagaa tacttttttg ctgatttctt cataagctcg gctttctttt
     1021 ccccctagaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
     1081 ccgctaattc gcatagtatc tttaagcatg gaaacatctt ttttaacatc tttcatttct
     1141 tctaattgat agatcatcat gtcttcaatt gtttgaggaa cagtattctt ccgaataaca
     1201 tcttccattt cgttgaaagc ttcaataaat tttagtttga aatttattgc tttacttcca
     1261 gtgaatccca tagctagcaa ggaaaatccg tctctattca tgaaataaac tctccgactt
     1321 cttccgtatg agtctggctc gttcccttcc acaaacatct gtccaaaatt ggacccatct
     1381 tcaacattgg ccgaattttc gaccgatctt tttattgatt caattgcttc tagtacatgc
     1441 ttatgtttct ttttgaaact ttctgccact tgtaagctcg tagttacagc ttctttattt
     1501 ttcaaaatta ctaattcttg cattatttct tctctccttt ttggtataat tttcgttaga
     1561 aagtgaggtg attctatgag gaaaaagctc aattacattc ttactgcgac tttaaccata
     1621 tatataattc tgtttattac cttatctttc ctgtcttcaa ttaaaaaaat tccttttatt
     1681 acattgaata aaaatagtat tttgaaggag ttcaataaca ctgactacat cgcatttcta
     1741 aaactttttt cagtctgtgt gctaatatca tgtgtaatta tttttattgt tgctaaaaca
     1801 tgttggacaa tgttaaataa aaaccaacgc gatgttgaaa atattgctat atttttaatt
     1861 aagattactt cctcaatatc aattatcatt ccgatgactg taactttttc aaaagaacag
     1921 tttaatgtag cggctactta tctttcattt atagcgctat tttcttttgt tcttcctaaa
     1981 agaaataaga gcaactttga caacaaggag ctggatgaag tacgcaatag cagcccaaag
     2041 gaacaaaaat aaaaaaatca tattttagtc agtccctccc gactggcttt tttcgctctg
     2101 tgctcagctt catcaagccc cataaaaatc caaaccatgt aaacgattgt ccctatcaac
     2161 gcttgtctgc ttccccagag gcctaaagcg tagatgatta gcggtgcgct gaatactagt
     2221 gctctgttga atttgcccat taacttgcct ccttcaaaac atctgaaggc gataaacttt
     2281 ttacacgata tttgtttgcg tctttccact ttaaaaacca aataaaagta tggtaatgaa
     2341 taaatgtggt tgagtgacct ggacgaagta ttccttctgc aaattcagga attgtttcca
     2401 tttccttgca gtattcaact aaagtagttt ttgacatgcc gtggaacttc tccaagatta
     2461 aactttgtcg gtaccagtcg tctggtttta ctgccttctc agcagcttct atcagctctg
     2521 aaagtgttgg ttttttcact tattatccct cctaaaaatt atcgttccat actcgatgtt
     2581 taacatttct tgaagctctg attttgtaag aatgatttta tcttccaacg actttacctc
     2641 cctctacaat tcgtacatag tgataatcga atctataatt ctgtttgctt ctgcagaagt
     2701 ctttttaccg tttaaaatta aagataagta gcttttgcta attccaaatc tttcagcaag
     2761 catggtgtaa gttaagaact ttgaactttc gacatattct ttgatttttt ctctatctcg
     2821 ttgagtgatt tctgcaatgt cagtcatact aaaactcctt tctaaccaat ttcctctaaa
     2881 tccatttgag ggtaatagcc ctctgcgatt aataagttgt aaataaacac tcgccctttc
     2941 tgtgtccatt tggtattcat tacaacttta gtgccgccat cggctttcgg tatctcactt
     3001 gtgtgcgatt ttgtgtatcc ttggttcata tgttttcggt aaagtatcca ttgtccgcta
     3061 actttatgct gaacacctaa ctcgtttagc aatttgttta gagcaattgc cgacattccg
     3121 taatcagctg caatttgaga agtagctacc gtatctgtcg atgaaagaat cgtatctaag
     3181 tagctaatct ttggttcgta ctcggcaatt tgttgttcga gtaattggtt cttttcttct
     3241 aaatccgctg ctaaacgcaa tgcttgtgca aaactttgag gtacgtttga atagctacct
     3301 gtttttctaa ttgttggaag gacttcactc gttacccaac gtttaaattt tttggcagag
     3361 ggaagttttg attttaagat taaactgtaa aggcctgact cgttaatcaa aacaactgct
     3421 gttccattga cggtgaacga atcgttctgc gtcttatcct ccaagtccac atgatctcga
     3481 atagcttttt gcggattcga atatcctaga acgtctgcta catcttttcc tacaaaatat
     3541 ggttcatcat ttactaaaat agttcgaact tcgttttgct cgaaattaaa aatttgtggt
     3601 gtattcatat ttctcattcc tttctttggt ataatttcct tatcagtgtg acaggctgaa
     3661 ataattgtta aggaggtgaa aatttatgaa accagaattt gtaattacta tgcttgatgg
     3721 agaaaaaatc aaaatatttg ccgatacttt ggtcgttgga tacgatgatg cgtcaaatat
     3781 cgaagaaaaa gatggaatgt tctattcttc tcaaatttac gtgaatacct tgcaaggtga
     3841 tattggggta tctactcttg caacatcaag accgcttgta ggtcttatcg gctttttgtc
     3901 aaaagcaaca ttcttttctc tcggcgatga tatagattcg gatgaaactg tcatctataa
     3961 aacatcagct gttaaaagta tcggtttcaa atagtaattc ttgctcgttg atagcggaag
     4021 ccaacttttc ttccaacaca tcggcgagct ttctttttgt tggattcatt tcaaagttga
     4081 tagtcttgag aagcattttt gcatccttaa ctttttgtcc ttgcaattcc ttgatgattt
     4141 tatcggcttg gctatcaatt ccatgtgtta gatcatctat cattttttgt accttgattg
     4201 tttggctcat ttccttgttc attgtttagc ctcctttaat aaatgatttt ttgtattttt
     4261 taatttccat aaattcctca aattgctctt tggtattatt accacgacca tacatattgt
     4321 gaaattcttt atggcaactg acacaaagtg taacgccgtt tgaaatcgct gcggatttag
     4381 ttgttgcatg gctaaatcac gaatcacaag tatctactaa aggcgaatca atttcgccaa
     4441 aagaaattgt tcaagcttat ttagatattc attatgctgt tatctatggt cagcttccag
     4501 aagaccgtaa aaacgatgac taaacgtgag ctaaaatctc tgctatggct gcaaccatgg
     4561 cagagtcttc tttatctacc gcgtgttcca tcgccacttt tgcttgtttc agtatgtcta
     4621 atttcaaatc ttcgattttt gccgagactg ttttttccat attcttctct cctttctttt
     4681 atattcgtaa acaaatttaa caacttttta aaaaactgcg ttgactttat tagattaata
     4741 atctatacta aatgcatagt taaataagac atataaactt tgatttaaaa agctttcttg
     4801 gcggttggca tttattaatc aatagtattt tttgttgtct ttttagttgt taaacttgtt
     4861 tacaaaacat agtatagatt aataatgtat ataagtcaac tatttttaca ttatttatct
     4921 aaactttttt tgtaagcatt cagaaaggtt gatttatcaa tgaatactta cgaaataata
     4981 aaggagttag caaaagaaaa aggattatcc attagacagc tcgaaatgaa tttcgggtat
     5041 tcgaatggat atttaggaag ttggaaaaga caaactccta attctattga gttagctcgg
     5101 ttagctgatt actttggagt ctctgtagac taccttctag gtagagaaga aagagaaacc
     5161 cctaaacatg tggatttatc agaaaacgat actgtttttt cttttgatgg aaaagaaata
     5221 tctaaggaga caatgcgtaa agcgattgca attgctaaag ctttagagga aaatgaatag
     5281 ttggagtgat gggttgtatg tatttgaaat tgaaagaaat gttgagcgaa tacaatttga
     5341 aagtagttta catggaaatg aaagagcctg gcttctatta tccgaagcct agaatcattt
     5401 ttttgaatga aaatctatat ggcgaaactg cagaagcctt tcatttatct catgaacttg
     5461 cgcatttcac cgcttctcat tttgaatatt cagttttgta cgatacttct acaacttttc
     5521 actcaaaatt tgaaacagaa gcagataaaa tagctattct aattttatta aatatctata
     5581 ttgagaacga actgactgat gaatctcagt ttaatctcga aaaatttatg gagtattatt
     5641 ctattcagaa taagctcaga tacacttgtt atgctgtctg ccaatgctat tttaagaaaa
     5701 aatattctta cgcaaggcaa tatgtatgaa tacatcaaga attattaaaa aaaccgattt
     5761 cagcaaaaca ttaaaaaagc ccgtgctggc acacggactc atacctcatt tctgagatcg
     5821 caaatatatt ataacaagaa gtgaggaata tttaaatggc aaaaaaagtt atgggtcaag
     5881 acggaaaaat gtataaagtt agtaaacctt tttacaaaaa agtatggttt tgggtattag
     5941 cagtaatttt aattataatt attggttctg ctttaaatgg tggatcagat agtaataaag
     6001 caagtgataa tggtggcgaa aaagtaacta aatcttcaac ctctgcttca tcttctaaag
     6061 aagaaaaaag cgataccttc tataagattg gtgacactgt aaaagttggt gatgctgaat
     6121 atacactaaa tagtgtagaa ctaactgatg aaagaaatca atttgaagaa aaccaacctg
     6181 cacaagtagt aaaaattact tataccgtaa aaaatgatgg cgattcagat atccctgtag
     6241 gtacagatgt ggaagtatat ggaccagatg acaaaaaatc agagacatat gccaacgaaa
     6301 atacaatggg atctgttgct ccaggaaaac aaatggatgt aactgctcat ttcacgttaa
     6361 accaaaaggg agaaatagaa atccacttct ctcctttagt atcatttgaa aaagcagcta
     6421 tttttaaagc aactgtataa taaataaaaa acacgcccca ccgtccagag taagagcgtg
     6481 cttttagaaa acaaacctat ataggtctat ttgttatgcc tattatagca aatgatagga
     6541 gatgttaaaa gtgtgggttg aacagaccaa agatggccaa ttcaagttta ttgaaagata
     6601 tgtagatcct tatacagaaa aaacacgaaa aaaatctaca acacttacaa gtaattcacc
     6661 acaagcgtgg aaaaaagctc agaaaattct agataaaaaa attaaagaag cactcgaaga
     6721 ttacaataaa tcagatatca cttttggtga gctgtataaa gaatggtatg aatattataa
     6781 gcagcatgtt aaacgtacta gctatttgaa ggttccaatg atgatgaaac atgtttctaa
     6841 gcacataagc gatgatacga tcgttagaaa cattgatgag acactcatta ataagataat
     6901 tgaagatatg tatacgtttg gtgacctctc actgaactac acaaaacaaa caaaaacaac
     6961 tctatctgtt atgctaaact atgcaatcga tagaaaatac attcaaagaa accctgcgct
     7021 agcagttaaa atccatccta aaaaagtgga agaagaaaaa agaaggcttt ctatgaataa
     7081 aaaatatctg gagaaagaag aaattgatca aatactgaaa cagctatact ccaatcctcg
     7141 cagaaaacta cacggcataa tagctgagtt tctatattta actggtttga gatatgggga
     7201 attgctagct ttgcaaatga aggactatga ggatgggaaa atttccatta atgggacctt
     7261 agattacaca tctgtgaaaa tggataatgc tataaaaaca actccaaaaa atacttattc
     7321 gcaacgtgaa gtgcaattac ccaatcgtgc aaaagaattg attgaaagtg tgatagctga
     7381 caatattctt gcaggtagac ccacagatcc cgatcaatat atatttatat ctacaagtgg
     7441 cactccgctt gcgctgcact catttaacgc tatactccat aaagtagagg aagagttgga
     7501 attagaaaaa agtctatcct cacatatatt cagacatagc cacgtttcac tattatctga
     7561 attaggcgta cctcttaaag ccattatgga gcgtgtaggg cattctgatg caaacacaac
     7621 tctgtctatt tataaccatg taacaaaaag agccaaacaa caagtaattg ataaactaaa
     7681 tagcctttga tatttttgcc ccttctttgc cccttttgct taaaataaaa caaaaaaaga
     7741 agaaagaacc ctgttaaaat agggttcttt cttaattact acatcatacc gcccatcatt
     7801 gatggatcca ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct
     7861 tctgtcgtta ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact
     7921 ttagttgggt ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg
     7981 aaaccagtgc ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca
     8041 gcgttttcag cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct
     8101 gttgccacgt ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct
     8161 gtaccaccac ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct
     8221 tcaatgcgta attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca
     8281 gctacaccac ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa
     8341 gttgtttcag caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct
     8401 ccagaacctt ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct
     8461 aagttttcga ttgttgcatc tttcaattct aaacctagat cgtcagtaat tactgtacca
     8521 cctgtcaaaa tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct
     8581 tttacagcta ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct
     8641 tcaccatcta catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct
     8701 aacaatggca gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg
     8761 ttttctagaa cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca
     8821 cgatcaaact gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg
     8881 attgtgatga caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact
     8941 ttatcagaac ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca
     9001 gttgagatat tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga
     9061 atgcctagag gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt
     9121 gtcagaacag ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact
     9181 tctgatacaa gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg
     9241 atcgtcacac catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt
     9301 cggcctttag gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac
     9361 atagctgcgc gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat
     9421 tgattatttt tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt
     9481 ttcctgcgac gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt
     9541 cgaacataac ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca
     9601 aacggccttc accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag
     9661 ctaaaacgat tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat
     9721 cacctaatgg ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac
     9781 tcttaacctt cgagtgctaa cctacatttt taataatact cattctccct cttttttgca
     9841 agcttttaaa tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat
     9901 aattgaaatc aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat
     9961 tacatgtctt taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc
    10021 tcacccttga tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca
    10081 actgcagtca tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt
    10141 gaacccttag cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat
    10201 ggattggttg gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct
    10261 gtattgtttg gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg
    10321 gaagcacccg cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta
    10381 ttccgccgca gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg
    10441 atcagctctt tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc
    10501 ctaggtttct tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc
    10561 acacacgtag gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta
    10621 gtataaacat gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag
    10681 cgacaggagt atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt
    10741 ctgtctttcc tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa
    10801 aaaactaatt gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa
    10861 acccgagagg atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa
    10921 tccaaaatat aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg
    10981 aacagccatc atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt
    11041 gaaaaaggct ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa
    11101 aaccgtcata aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg
    11161 atcgagaaat tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga
    11221 ggctaagatt tctgcatttg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt
    11281 gaccatcgca gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa
    11341 aagcacacca aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac
    11401 tccttgaaca acagtcattc tgaacaaatg ttgaaaaacc gctgaaccga taagcgtaaa
    11461 agtgactata acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat
    11521 cttctctctt tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat
    11581 gatgagccca gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat
    11641 ctgagtattt agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg
    11701 taagcgatcg atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa
    11761 ggctatcaca tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt
    11821 gtctatccac tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc
    11881 aacaataatt attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag
    11941 attcacaaca gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta
    12001 tctccttcaa cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca
    12061 cgaaccatga aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc
    12121 agcaactgct tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat
    12181 tggagctact ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga
    12241 cgtgacggca atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa
    12301 ggatttattt gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt
    12361 caacgctccc aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat
    12421 gcttgaagga accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga
    12481 tacagaaaag acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac
    12541 agctttcaat atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac
    12601 ttatatcagt ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt
    12661 aagtgatgat tatttacaaa gaaacgctgc tagcgacagt acgatcagca tggcagatta
    12721 cgcgattgca ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt
    12781 agtcagtcga taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa
    12841 accaaccctg attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa
    12901 tcgaaaatag ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa
    12961 cgccaaaatc tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat
    13021 tgttttcctt gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag
    13081 tcatgacaag tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt
    13141 ttaaattcca cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca
    13201 aataaccatc ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg
    13261 tcaacatgac gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt
    13321 catgggagac gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct
    13381 caaatagatg tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag
    13441 tctgctgatt ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta
    13501 tatgatctcg atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa
    13561 tttgagccat caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt
    13621 ttttttcact ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt
    13681 taaaagctgg tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt
    13741 tttcattata ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga
    13801 tcgtattgct gaaagcgttg acgctctcaa tttggaccac tttatcgctt gtttcgatcg
    13861 ctacatgcca aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag
    13921 ctagctgacg ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat
    13981 tgattgcttc ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata
    14041 aatgcgtctg catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa
    14101 cgatttttgc tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt
    14161 ctagtggttc ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata
    14221 aaatattgtc tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga
    14281 tcacgttctt ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa
    14341 acacaccact tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc
    14401 taagattggt ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc
    14461 ccgtgtaagt gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc
    14521 gaatgacatt gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca
    14581 accctaaaaa agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct
    14641 ccaatccagg taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca
    14701 cataccattc aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat
    14761 agccaacgat cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga
    14821 tgatttcatt cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg
    14881 tattcaaact ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca
    14941 caccaatctg tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat
    15001 aatcgatcgt attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt
    15061 tgctaatata ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct
    15121 ctccacgaaa atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt
    15181 aaaaaatcaa ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag
    15241 catttctaaa aagtgtatag ggttgctgtg acaggatata actatacttc ttcagtttga
    15301 tcgaatgttt cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc
    15361 attttgtgtc ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg
    15421 cttcatatac gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga
    15481 attgtattct agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat
    15541 ctaaatcaaa tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa
    15601 aaccagagtt cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag
    15661 aaagctttcc aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac
    15721 tgttggtttc ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt
    15781 cggaagtttt tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga
    15841 ttgaactttt tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa
    15901 ccatcaaaat gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc
    15961 ctcataaggt tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc
    16021 tgcccaaata gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc
    16081 tgtcccacct tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg
    16141 gttcagatct tttttaggaa tccccaatcc ttgatcggtg atacttaaaa tcacgttatt
    16201 atgcgtttct aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt
    16261 attcatgata ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc
    16321 tctttgcgta aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc
    16381 aaaccggtca agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg
    16441 ggtattacct gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg
    16501 gtctgtttca tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc
    16561 accttcgctc aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg
    16621 ggaaacatta gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc
    16681 atgcaagaca gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc
    16741 tactcgcaag atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc
    16801 gtttggatct tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa
    16861 aatcgattgt ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt
    16921 gatcgtaatg actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag
    16981 cacgctatcc aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc
    17041 agctaactga ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac
    17101 tttccgactg tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg
    17161 ctgtgtaatc gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag
    17221 tgaggcagta aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt
    17281 acttttgacg taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac
    17341 attgatattc acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc
    17401 attgatatca acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt
    17461 tcctcggatg atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact
    17521 gttttcaata aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg
    17581 attcaattcc gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat
    17641 aaatgtattg atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat
    17701 cgagatcaat aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa
    17761 gcggactttt cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac
    17821 cacgacgtgt cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac
    17881 gacgtacagt tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt
    17941 gtaataaatg ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt
    18001 cgaattcacg atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg
    18061 gatggatcgt caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg
    18121 cagccgtggc accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa
    18181 atggcttggt cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag
    18241 aatctttggc tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa
    18301 cttctaagcc gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct
    18361 cagcaacttt ttccaaggct tcttccccat catatgcagt ataaacgtca tagccttctt
    18421 tggctaaatt gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt
    18481 ttttcataaa gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc
    18541 tgcttttgtt tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat
    18601 actttattat tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt
    18661 gtttcctttt tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac
    18721 cagtgtggac tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga
    18781 actctctttt aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa
    18841 cagctaagtt gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat
    18901 ccaaactttt tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac
    18961 atgagatgat tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc
    19021 gtccaccttt ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga
    19081 ctaactcatt cagattactg atcagttgat cccattctac tccttcttcc agccattttg
    19141 ctgcttggat cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg
    19201 cttccaattc ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc
    19261 cactggaaat tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga
    19321 tttctgtgat cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt
    19381 gatagatctc ttcaggggta atgtcaattt tatctgtata tacctgatct ttataaataa
    19441 ttttcaatgg gatcatatac atctcgtatt tttttatcaa ttcttctgga acatccgtac
    19501 ctgaatctac taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat
    19561 tcggtttcta gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc
    19621 gctcttacag caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta
    19681 ccttgtgcta attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta
    19741 taggcttcac gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga
    19801 atagtcaata cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta
    19861 tatctttttt taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa
    19921 gaggtaagca atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac
    19981 gttctgactt ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg
    20041 aatgtttcta gttccttccc ccactccatt aattgctgac gcattttttc catcataacc
    20101 gctccttttt tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg
    20161 ctttaactag gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc
    20221 cttcttgtct tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta
    20281 aaaaatgaaa aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt
    20341 atataatagc gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt
    20401 tttctgattt tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa
    20461 gtactttttg tacgggccgt tagctcagtt ggtagagcag ctgactctta accaaatgct
    20521 ctatttgtga aaaaagtttt ataaagcttt ataaataggt ttttagtttg tgaatttttt
    20581 cattgccaac atactgccaa caaattgcca acaaaaaaat tctaaaaaca agaagcgata
    20641 ccccaaagat attgtttttt gtttttttat tctttaaacc caaatgcgtt ttctaatgct
    20701 tctgctgcac gatcttttac tcctgcggtt aaatgcgtgt ataaattagc agttgtcgaa
    20761 tacgatgcat gtcccagcca ttcttgaatg gatttcatat tctggccact atctaacatt
    20821 attgaagcat tagaatgacg caagtcatga aagcgaattt ttttcaatcc atttttctta
    20881 atcaacttac tcaaacgatg cgttacatat tgaggcttca tcaaccgtcc atcttccata
    20941 gtaaatacgt actcattatc ttctaaaata taatcatctc ctaaaagaat acgattatct
    21001 tgttgccatt tagcttgatt tactaatgct cgttcaatcg tttcaaccaa aggtaatgta
    21061 cgatagctgg aagagttctt tgtttgatct ttctttatta actgtgacat attctctgga
    21121 ttttctatga ttacatggtt tatcatgatt tttttgttta aaaaatcaat agcagaccat
    21181 tttaatccta aaacttcact tcttcgcaat ccataaaatg ctgttaaaag aactaccaaa
    21241 tagagttttt cttctgacaa aatgtcaagt aatttcaaga tctcttgttg attataaact
    21301 tgagaaacaa attgtttctt ttttggcttt cttactttat ctgctacatt tgtacttatc
    21361 aattctgtaa taacagcctc ttgaagagct ttgcgtataa atgcgtgata atgaataacc
    21421 gtatttgctg tacagttatc atttaacata tattgataaa actcttgtaa atgataaggc
    21481 tttaacctat ttagtttaat ttttttagga taaaaaaact tgtaaattcg attatggacg
    21541 taatgcttat acccaacata tgtgttaaat gctacttgtt tttttgatac ttccaaataa
    21601 ttaagcaaat agtcataaaa caataaattt ggattagcac attcacattc catagctaat
    21661 tgactttcca attctagtaa aaattcatct ctaatttttt ctgctcgttt tttatttcct
    21721 ttctttttta attttgtatc tttccacttt tgtctaattt ttccgttaga gtctttataa
    21781 gatacaacta cttgtaatgt tcctcttttt tcaattatgt ttgctcttaa tttcaattca
    21841 atctctcctc ttaaactgaa atatcggcaa aaattttgac tattttatta tagcatgatt
    21901 tttaccttca tcttgtattt tcaatatata ctcaatgaca gcttttttag gtattttgta
    21961 ccttgctcca atttttatat tttctattga tctatctttc agcaaattat aagctttatt
    22021 ttttccaata tttaacattt tagttaaatc agtaattttc aatatcgatg gataactttc
    22081 aaatattttt tcataatctt gaaaatccat aaaattcact ctcaattctg ataataagta
    22141 ttgcactttt ttattttagg tgttagaata acaaagtatt aatttgggct gttagctcag
    22201 ttggtagagc agatgactct taatcatcgg gtcgcgggtt cgagcccctc acagcccatt
    22261 gggtgccaaa cccttaaata cagtgtaaac gttggtgctt agcatttaga aaacgtatat
    22321 actgtatttt tttgttttca aaagagactg tagtacccct ttttttcact aaaagcccac
    22381 tgtagtcatt gatatttttg ataagaaagg aacataatta tgcgtaatga aattgctaat
    22441 ataacaataa tccgagaaac tttacaaaac catactgcaa atgagatctc taaacataca
    22501 ggattaaatt tgagtactat aaaaaaacta aaaagtggag aacgttcaat agaaaaacta
    22561 aatttacatg atgctatttg cctcactgaa tttggtctta aaaataatag aaaaaatgtt
    22621 gaaatcaaca tatggaaata attcttaatc agctgctttt gtcaaaagca cataggaatt
    22681 tcacctctct ccaagtttac ggcgagcctt acggaagtat cattatccca acttcaaatc
    22741 atcgcttttt attgactata aaaattattt tagaatgttt atcgctcctt ctttacagta
    22801 agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt aacgtactga tagaatgtat
    22861 ttaaatttca atgatcaata ggttttctgt ttaaaagaat ctactaatcc tttaactatt
    22921 agaagattct tttaaacaga ataagtggaa aggggacttt ttagttagtt aattttaaat
    22981 tttaagatcg cttctatcaa tttaatttct agctgtctta gcatatactc atctacatag
    23041 gtaataaatt gccccgcctc attaaatcca tttgtcaaac ataacttagc tatataacta
    23101 tgataatgtt tgagtatcat cttgattgaa attggatctt cttctagagc agattggata
    23161 atggatagag gaactaatgg atactgccgt tttttggggt tgtctaccat aagtcatacc
    23221 ccatttttaa ttgttcaact gccttctttc gttgatacca gataccactt gtactcatat
    23281 tgaggatttt accgatttca gtatcattaa aacctgcaaa ataataaagt aggataacct
    23341 ttcttcttat ttccgataaa tgatcaatgg ctgccgataa cttttgatct gaaattaata
    23401 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gagtacttac
    23461 cttcatttga attcgtcaat ggttcctcat tatctgttat gttaatgttt cgttttcgaa
    23521 tagacttagt ttcgtttcgt aatacttttt tacagtaggc atcaaattga tgttgtatag
    23581 atttctcatt gtaattttgt tgcatgataa atatgctcct tttttcccct ttccactaat
    23641 acaacaatca gaaagcgcct ttttttcgaa aaaactagaa aaaaatatcc ttctttaaac
    23701 agaaaatctt tttataaaaa aaaggagttg gcaattaagc agccactcct tttttatatt
    23761 ccttataaaa ggtaaattat tttttataac tgaaatttgg tattgatgac catcgtttct
    23821 ttagatagtg agcaatcatt ttaggctttc tatctctagt aaagattcct ttcttatttc
    23881 cttgcactct taaaatccct tgacttgttt caaaatctgc aaaattccac acttgttcac
    23941 caacaaaatt gtcacattta tctattactt cgtgatttgc ttcgtaataa cccacttgat
    24001 actcttctgt aaacattgtt ggtactgtat catgaagtcc agctactgta tctgctccat
    24061 attctgtaaa cattattggc gtatcgggac atctttccat ccatccatct agctcttttt
    24121 tcaacagata ttttgcatgt tttaggtctc cacctgaaac ataccaacca taatagcgat
    24181 ttaaacataa aacatcgaca atatctccta ctttacaaat atctggtgtt gactttaagt
    24241 aagtaacgat tgttacaggt ctcttctgag ggtcaagttc tttagtcaat tcaactaaag
    24301 gttcaaaata ttctttagcg ccttcagaat ccgaatctgg ttcatttgca atagaccaca
    24361 ttacgacact cggatggttc ttgtcgcgtt ctatcaattc cttaatcgcc attttgtgag
    24421 cattttctgt ccgtatttct ttccaagtat catgtttttc tacgttgtca tctaataaag
    24481 tagccatgaa attcaaatgt aatcctactg caggtacttc atcaataact acaatacctt
    24541 ctcgatctgc taagcgcata atttcttctg aataaggata atgtgaagtc ctaaatgaat
    24601 tagcaccgat ccatttcatt aaattaaaat ctttgatatt tactgcttca ttcaaaccac
    24661 gaccattaat atacgtgtcc tcatgcttcc caaatccctt aaagtaaaaa ggtttattat
    24721 taatcaaaaa cttcccattc gaaacctgaa tactacgaat gccaaattct tctgtgtacg
    24781 tatcaatcac tttattttcg ttaattaaac taactttcat actatataag taagcattta
    24841 gaggctccca aagtgttggg ttcttaagtt gaatcactcc ttctttcccc ttagcttctg
    24901 ctataagttg agcttcttcg tcatagattg ctattagaat ttctccggtc cattctgtat
    24961 cgatattaaa ttctacattt gcatctgaat caattatttt tgtattaata gtgacatcct
    25021 ttatatagtc tttcggagtt gtatacaatt taacgggtct attaatgcct gcataattga
    25081 aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc atcttcctgt atcgtttctt
    25141 tcaaaatccc tacaggtaaa gtgctatcgt caataacatt gtttacagcc actgtaattc
    25201 tgttacttcc atcttttata aactgattaa tttctgattc aaatggcgta aaccctcctt
    25261 tgtgttgatt aatcaatttt ccattcaaaa atacttttgc ttcatgtgtt gcagatccaa
    25321 aacgaagaac taatctttct tctaataatt ttttaggaag aatcacttct ttttcatacc
    25381 acacccatcc aacatgattt ctaatgttct ctgtttcaaa taaatcatta taagaagaag
    25441 gaactgaaat tggataactc tctttttcaa ccattggttc taaagcaagt tcttcttgta
    25501 agtcatcatt tgtatctaac ttaaatctcc aaagaccgct taaatcaatt acttgcctcg
    25561 tctctgtcat aatgggatat aacatgtaaa tcactcctat ataatttaaa tattaaataa
    25621 atgtatcctt taacccacaa taagcatcta ttggacttat tcccttaaat tcagaaaggc
    25681 ccattagttt gtccactaat atttctaaag tttcttgttt tccatcataa gtattgatat
    25741 aagtttttac ttgaggcaca tcagctaaat gaaacggaca ctgtacagat acaaaaattg
    25801 ttggaatttc atgaatataa aatggaatat ccggagtacc tttacttgct ggccaaacaa
    25861 ttctttgaac tgtacctggg ttaacattag ctatattaat aatcaagtca tatttttcag
    25921 ttaaatcttt gattggtctc ttagcagcat atattccagc tatattttta attttttctt
    25981 cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact tcaaatcctt
    26041 ttgctgacaa aatctgtttc aataaatcaa tggcctgagg tctatttcca gcaactactt
    26101 caccaaaccc accttttacc ccatgtacat ctaccagtaa aatcctttta tatttttctg
    26161 gtacaatagg ccaaatatct tgtttatctt taactagtgt aattgcttga tcagctacct
    26221 ctttaaaaat tgatttattt tcaattaaac caattttttc catagcttct tgtttcggta
    26281 acaaaatttt attcttttct tttttatgca aacctagttt agcctttaag cctaaaattc
    26341 ttcttaatgc atcacgtaaa cgctcttctg aaataattcc tttgtaataa ccttccatca
    26401 tataattaaa gtcttcatct ggatcattaa aaaataggaa tagatcacat ccagccgcaa
    26461 tagaagtggg caataattct gaccgtttca ttgccccagt cattcctaac atatgactag
    26521 catcagtaac tactaatcca ttaaacccga gttttcctct aagtaagtca gtgattaatt
    26581 cttttgataa agttgcagga agtaaatctt catctttcag atcaagatta aaatgttttg
    26641 aataagctgg catatggata tgacctgcca taattgaagg taatccagct tctattaagc
    26701 ctgagtatac ttttccaaaa gaattatccc attcttctgt acttaaacta ttaatactaa
    26761 aagatagatg ttgatctcgt tcgtcaatac cgtcaccagg gaaatgttta gctgcaggca
    26821 aaattccact ctcttgaata cctttcatat aagctaaact aagttctaaa actttatcag
    26881 ggctttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta ttaatatcta
    26941 caataggagc aaaactccag ttacatccaa tagccgatgc ttcaattcca gaaactctcc
    27001 ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct acaccaactt
    27061 cggtaccatc tgtacaagct ccgttaccac ctgcttctgt attagcagca attaataaag
    27121 gaattttgct ttctgtttgt aggatatagt tttgatcata tacctcatca gcagttcctg
    27181 gattatatcg tacagctcca atatggtact gattgaccat actacttaag taatttttat
    27241 cacgactaga tcccatatta acaaataatt gacctatttt ttcttctaaa gtcatagcat
    27301 caatcgtctc ttcaatccaa gaaatagcat cttcatctaa atgatatggt ttttttcgta
    27361 agtctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt ttccaaaaag
    27421 tatctatata tttttgttca atatctatat actctcccga taaacattta aataaaatat
    27481 cagttcttaa gttttcccat gattcttttt ctttattaac caaaatagga taaaagaaaa
    27541 ctttattctt atttgcagct tctagatcac caggagaatc accaatcatt aatatcttat
    27601 ccaaatcaca accattttca attacacttc gaataatgtc ttcttttttt cctttatctt
    27661 gacaacacag ttcatcaact aaggataata attcatgtct ttcccattct tcttgaatag
    27721 cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga gcttttatag
    27781 tatctctagc acttaaaaag ggtttatcta atcctttagc ttgactaatc tgtcgattga
    27841 cttcttccga ccattttaaa gcttttaata aatcctctga ctgagagtat tctatttccc
    27901 tcttcaaaga tggattagac aattcttttg ttacagatac ccagtgcttt aatcgattaa
    27961 tcgatggtac atcatttttt tctaatgttt taactaaacc taaaaatctg tttattccac
    28021 gagtctctga atataaatta atattattcc aatcctgtag gaatttttcc ctatgatgaa
    28081 catcaaaaat ctctgcagca attggcccga aaaagagatt gtgtttataa gtcattgtat
    28141 ccatgacaca accatctgaa tcaatacaaa atatccaact gttcatgttt tttctcctag
    28201 ttatgttttt gatttagcaa attataattt tctaatactt tttctttaga taatggatat
    28261 acgaaaaata aaatcaatcc tacaacaaag aaacaaactg ctggaatcaa cgttgaagag
    28321 ttatacagct tttgtaaaac ttctccactt tgtacttttg ctaacgaatc ataccctatt
    28381 atagaaagag tccaacctcc gacaccgcca gctaaagctt gtccaatttt tcgggcaaaa
    28441 gaatatactg cataaatagt tccatcttct ctttggccag atttcacttc ttgatcatcg
    28501 attacatcag taatattcgc ccaaatgact gtattaaaaa tacctaaccc catatatcca
    28561 acagaagtaa gaactaagaa tacatacata ttactaggct ttatcaaaaa taaaagtaaa
    28621 tatacaatag cagagaagaa catccctact gacgccaatt cttttttacc gtatttttta
    28681 ctgaaaatta aacttacagg tgaaacaagt accaacatca aaataggatt gataaagttc
    28741 attaaaacta ttccctgtgc attattataa taatctggaa atacataatt gttcatactc
    28801 attgttaaaa gctgagcaag caacaaaaaa atagctgcag ctattataga aagcaacgct
    28861 ctatttttag ctaatccttt taaagatttc aacaatgaaa cttttaactg cgattcatca
    28921 cttttaaccc gttctacagt taatttataa catattacgt aacaaataac tgcacacact
    28981 gaaaatactc ctgccacaat tgtaaaagtt gatccaccac gtacaatttg agctccagaa
    29041 ctatctcttg tataaataaa aattggtgtt aacacgccaa tgaacatacc tgctaaggta
    29101 gctcctatac tacgaaatgt tgataacgct gtcctttctt ctgcaacaga agtgatagct
    29161 gatgccattg aaccgtaagg aatattaata gatgtataaa aaatacttcc ccatagtata
    29221 tatgttacat acatatatat aactttaata ctcatagaag ctgatgccat tgatgtttga
    29281 tacattaaaa aacttgctaa agcgactgga ccactcatcc gtaaaatcca cggtttaaat
    29341 ttgccagttt tagatggttt tgatccatcc acaatacgcc ccattgttat gtctgtaact
    29401 gcatcaataa atcttgctac taaaaataat gttcctacgg tagctccgct tatccccaat
    29461 acttttgtat aaaaaatcat taaaaaagaa ctagcaaaaa tgaaagtgaa atcattccca
    29521 aaatctccaa acatatatcc tattttgtct cttagaccaa attttctttc catataattt
    29581 gcgctccttc ctattgttaa gcgttttcat tctgttgaac tgccaactta cgtactatac
    29641 tctgaagtat aaagagttag tatttatttt ttacatttta tagtattatt tattttttct
    29701 cctaataatt ctttatctca taaaacttat ctatatattt actagctatt cgagttactt
    29761 cttcaaaatc acctttttca acaggagcca acaaattccc acctacacca actgcaacaa
    29821 cgcctgcttc aaaccactct tctaagttat ctagacttac tccgccagta ggcattatat
    29881 ttacatatgg taatggagct tgatacgttt ttacaacaga tggaccaaaa gcagaacctg
    29941 gaaatagttt aatgatttct actccagcgg taagtgcctc ctttatctca gtaattgtca
    30001 tacaacctgg taaataagga atttgatata aattacaaat ctctgctgtt tgtcgatcaa
    30061 aagatggact tacgatatat tctgctccag ccataattgc aatacgagca gtaaacgcat
    30121 ctaaaactgt accggctcca attattacat ctttttgatc agcatatttt ttagcaattt
    30181 ctttgattat ttcttcagca ttcggaactg taaaagttag ttcaatccct ttaattccac
    30241 ccgcagtaat tgcatttatt gctttaatgg cctcatcttt attttttcca cgtacaaccg
    30301 caatgactcc agccgcttct aacttactta aaatttctac ttttttcata tctatctcct
    30361 tcttttatgg atacatactc tagctagttg tactaactta caataaataa atttaagctt
    30421 ttattttttg tattattttc tactttcgat ttcaaaaaaa ttaaaataat tatacgcatt
    30481 acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg gaattcgacc
    30541 tgctttaacc cactcgctta gtaattgaca taatactcga cggaaatact catggcgagg
    30601 atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca acaaactttg
    30661 ttgcgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga accaccaagc
    30721 acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg ctgttgctag
    30781 tggaagccaa tcggttggat ttaatgaata taaaattgtt tttggtaagc acccactctt
    30841 ttcagcagtg tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat cccccatcga
    30901 atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat ttcttaaagc
    30961 atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca aatgtaattg
    31021 aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc ctgctaaagc
    31081 ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat aagtattcaa
    31141 accgtgatct gctaatcgac agccaatttg atgaaaataa tccactcgtt ggctcaatgc
    31201 ctgcttcaac ccattaaagt ttttgattgg tatacccgct gcttgactaa gttttaaaat
    31261 atagttcgaa aatcctgatg atttgatact ccagacctta tctggacgga aagtaggtaa
    31321 taccttgaaa gaattatcct gtttagaaag ctcagtatga tatcgcaagt catctgtagg
    31381 atcatccgtt gtacaaatgg ctttcacatc catcatcttc agcaattctt ttgggcgata
    31441 ggaatccgtt tggatttttt cgttggcacg tcgccaaatt tctggtgcat tcttctcaga
    31501 aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt caaaaatagg
    31561 attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat aatcattacc
    31621 tgcaccactg atcaactctt cttctatccc attagctcgc atcaatcgcc atttataatg
    31681 atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt gcttattttc
    31741 tagcaaacat gctgggtcta aatgacaatg ataatcgatg attggttggt gttctgcaaa
    31801 tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat ctaaaaacat
    31861 atttaagcac tcctttcctt cccttagttt ttagaagaga aaatttttct tctaaataga
    31921 ctccattata taattattta ctcgccccct tttcaagcgc ttcccaaata ccatttaaat
    31981 aaactgcacc tagagctcgg tcatataacc cataaccagg tctaccggtt tctccccaaa
    32041 tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac attgttttta
    32101 atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttca taaaaatcga
    32161 gtccattatc acttaaatat ttgatattac gaacatgtgc aaaaggaatc cgatcccgtt
    32221 gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca atacttccag
    32281 tacataacgt aataccatta gaaggagaat ccactacatt acacaaccat tctaaatcgt
    32341 cacgattttt aacaatacga ggtaatccga aaatagaata tggtggatca tctggatgaa
    32401 ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga aaatatgcaa
    32461 agttctctcg caatttcgct tcatcaactt ctttataggc ttctaataat tgcttgattt
    32521 gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa ttttctactt
    32581 ttctaataat ttcctctgga tcagtaggaa tattttttga aatataggct aacgtagaag
    32641 acccatcatc taaatgataa tccaaatctg attttaccca atcaaagact ggcataaaat
    32701 tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt ttataattct
    32761 cgatgtattg atctcgtgtt ggcaaaccaa ttttgatatc atcatgaaca ttaacactct
    32821 caataacttt caaggtaagg ccattggctt cgacttcatc ttttaaagct ttaattcgtt
    32881 ctctcggcca gacctctccg gctggtatat ccattaatgt tccaacaatt cctttcattg
    32941 ctgggatctg acgaatttct tgtagtgaga ttttttcttc atttgcccca taccatctaa
    33001 aagtcatttc catagttact ttcctccaac ttctgttata tatttattga ttgttttacg
    33061 aactgcttga ggacctacta gcatttcgca aaagattttt tcaattttct ttcctatctc
    33121 aacttgatat aaatctaaac caaatatttc cttattttct aagatttcct gtaccgcaca
    33181 atgaatcttt gcaggatctt gttcacctaa tttcaatact gagaattttt cctgcaactc
    33241 taccaataaa ggatcaggac ttggcacaaa attttttcct tgatcatcga ctgccaataa
    33301 ataacgtaac caagcagcaa tagcgatagg aataaatgtt agattttctg catttccgtc
    33361 tttttcgaca tactttttaa ttgtttcacc atagcgaatc gctacttttt gagaagtatc
    33421 tgttgcaatt cgttgcggtg tatctggaat atagggatta ggcaaacgtt tattgattac
    33481 ttcatcaata aattgttttg gatcaataac gcctggactt tcaactactg gtaagccttc
    33541 tacataacct atccctttaa ttaagccaac caaatcatca tcttgcattt ctgcctgaat
    33601 tgatttatac cccaacaaac aacctgttat tgctaatgcc gtatggagcg gatttaaaca
    33661 tgtcgtaacc ttcatgatat ctgctttatc aactgtttct ctatctgtca taatcacacc
    33721 agccgcttcc agtttaggtc gaccatttgg aaaactatct tcaatgacta gataatggac
    33781 tttttcagta ttggtaaaag cagcgatatt ggtatttttc gcagtatgaa ttatatctaa
    33841 gtcttcgaaa cccgccttca ataaatccat tttgacactt tccgaaggat ttggcgtaat
    33901 tctatcaatc atactccaag gaaaagtcac tttttcttta tcctttaaat aagcaataaa
    33961 tttttcagaa acgtatcctt ttttttgcca agcttctgca atcataaaaa tgctatcttg
    34021 aaaacgttga ccattctgtg agaaattatc agtagaaacc atcgcaatcg gtaactctcc
    34081 agcattaaac ctgctataca acaaggatgt cacaatactc atcgtatgtt gaggattggt
    34141 aggtccagat tttatatctg cttccactac cgaaaaaaat tgtcctttag aatcttttaa
    34201 actgtatcct ttttcagtaa tagtaaacgt tgtaaactgt aatgtaggat cttcaaaata
    34261 tttaaccact ctttgatagt ccgttatatt ctttggattg caatataaac tatcagctgt
    34321 tactgctaaa agtctttttt tgagttcacc atcttcaccc ataacaatct ccaaaatatc
    34381 attgttgtag tcatgataag ctttttctac tacttcttca tcaaatgtct cacacacaac
    34441 tactccggat tctaattcat tttgatctgc taagcattga gcaatttctg catgaaatcc
    34501 tcgataaaga tttcctccac caaaatgaag ccacactgga tgtttctgac cttcttgttt
    34561 gaccgcttcg acattaaaat acggtagttg aacttgcatt ttgttcaatc cttcgataga
    34621 atcttgccag tctttaatca aaaccatatt tacccctcca tactgatatg ttagtttccg
    34681 ttacaattgt agtataaaat cgaatgtaat cgctgtcaat aagaaaaaac taaaaatatt
    34741 cttttttcta ccatgtcaga attattgtgt ctttttttag taaaagcaca ccaatatata
    34801 ttttttagaa ctaacttaaa ttaaagctta atttctcttt gatcttattt gagtattttt
    34861 tgttttttta atagctagta tgttactttg aaatttgata ttatataacg agcatagaac
    34921 taaaatatca aattcaaaaa tgaaattttg agagggatta aatatgacaa ttaaaactag
    34981 tctccaaaat caagcttatc aatctattcg tcagcaaatt atctacgctg acttagaacc
    35041 tggaaaaaaa gtatcagaaa aaaaattgga agaaacgctt tctatcggtc gtactccaat
    35101 acgtgaagca ttgattcaac tccgtcatca aggacttgtt gatacaattc ctcaaagtgg
    35161 aaattatatc tcattaatcg acttagattt agcaaaaaat gctcgttttg tcagagaaca
    35221 tttagaacga caaattatgc tagagtgttg tgccaagatg aataatcaaa agaaacaagt
    35281 tttagaaact ataattgcag aacaagaaaa agctgttagt caaaagaatg ctcgtagttt
    35341 ttttcataat gataatttat ttcataaagc ttgttttgaa attgcagaac gggaagaagt
    35401 atggcaatgg ttggaagata ataatacgca cttggaacga tttcgttggt tacgagtaaa
    35461 aacggaagga ttaaaatggg aaaaaattat ggaacaacat catcaactat ttaaagcctt
    35521 gaccatgcaa gaccctgaag aagctaactt cttaacatcc ttgcatcttc atttaatgct
    35581 tgatgaacaa aatgttgtat tgactactca cccaaaatat tttaaaaata tataactgct
    35641 aaatttcttt tttttaattt atttacattt ctgattacta cagaaaacta actacttttt
    35701 ttatgtaaag ttctgtcaaa tatagtcact ttatattact ttctcttgag taaaaaaaca
    35761 tgtgaggtcg taaacaaaga ttgaactatc tacattcata ctctataatc tgtttatttg
    35821 cagactgata atagcaaaag aaaacttata atcaaataat atcaacattt gatcacataa
    35881 tttgagattg aatgctgatt tacctatgtt ttacgtaatg gtctttagtt atttttgctc
    35941 ataaaatgca taaaacacgc gtttttatgt gtgcttgaaa tttcttggct ctataatttc
    36001 taagactgat aaaaatattc tatcggttct ttttgcaaat aaaaaaacat atagaagctc
    36061 cattagattg aatcaccaca acacaatcaa aggagcattc cgtatgcttg attttcattc
    36121 tataaaacta cttttaggaa tcaaagaaaa tcacaacggt tcaaactaaa agcaagaata
    36181 tccgaagtac cattgtttca tcgatcatga gaaaagcatt cacctctgtc tgcagatgtt
    36241 cttatatcgc tctgtcagtc ctattaataa agtactgttt tgttttcaaa aaaactatta
    36301 cgagtacttt ttaagagaat gccttgcaaa tattcatcac acaccgttat ataagtcaaa
    36361 tagtttcttt cagtcgtcaa gaaaagattc tcctgtcctt ggcattatca gttaagtagc
    36421 aacttaattc tcggacttgt tctactctag caaaatttat tttcatcaac ttacgtccaa
    36481 atgttttttt gaataattac ttcatcgcgg gtctggaaga cttgcctcat tcgataagtc
    36541 actagctttt ggcgtccttc aggatttcta gtatacacca actgacattt gggcagcgac
    36601 gattgattta attcgtttag aaaaaggtta gcgatttgct tatttcggcc aaagcgttct
    36661 ggtaccgtca gaactatctt tttcccttcg attaaatacc gcggattctc cagcggtgct
    36721 aaaagctccc tcatagcttc agtaaaaagc tgtccttctc gatagccagc attctcaaga
    36781 tagcatctaa attcctgctg atcagccgct acgattactt ttctatcact aacttcacca
    36841 aggcgctgca acgtcgtttg aacgcatatg gccaagcttt ttttgcgggc acattccttt
    36901 tcccatagct tttgtcgcct acgaatttgg aaatattcca gcaagttacc tttaaatata
    36961 gctgaccaga agcttagagg ctctatattt tttactacta tcggcaatga agtttcttgt
    37021 ttttgcttta cctgatcgac tttcctaacg acgattggca tagataggtg ccagccatca
    37081 gctaaagcag tactccattg ttctttcaac cgacttcgtt cacaagaaaa atagaacatc
    37141 gactgagtat aagtttccat cgtggtggtc gtatattcca gtggtaaagc aaagcgatcg
    37201 ccaccactct caatttttgg tgaagattca aaggtcagac ctgaaatatt gcccaaacgc
    37261 cgtagcattc ttttcaaatc aggctgttca gacagaggaa ctcccggtaa tccaatcgct
    37321 aaatgccaaa tgttcgttac tttattttgt gcacctaggc gtaagccccg ccctcgtagt
    37381 tgttgtgttt gcacaaaagt ggaagcttga ttagccaaga tcaacagatt aattgcagga
    37441 caatcccagc cttctcctag caaactaacg gttcctacca aaatattcaa cttcttttcg
    37501 gttaataggc gagtaactgc ttgcatcaat gcttggcggt ttttgtcatc caccgtcaaa
    37561 taaaaataac ccgacatata aggatgttca ttcatacccg tcgctagttc tgccagctca
    37621 acagttacta gcattttcgg taacagtaag aactctccac agatggcacc gatttgctga
    37681 gtgttaccta atagacgaac aacttcatca aacaaaggaa ccacgccata agctaaggta
    37741 ccatcactgc ctgctaatac ctccttgcaa atacgatcca tcaaaatgac cgctgtcagt
    37801 tcttgcttca gctgaacttc ttcttttaac aaaatttgac aaactgcttg ctttttctca
    37861 ggaatcgcct gactaccaac agtgtctgga tactctggaa acaaatgcag cgactcatca
    37921 aacaatcatc cagctttttt caattgataa aaaataggca attccggttg atcttcaaac
    37981 atctgctgat aaatagtgat cagctctttt ttacctaaag atggaactga agctttttta
    38041 ccttgctgct tagaactgcc taatagctgc caatgttcgg gtatcaattc atagttatga
    38101 tgattcaagt aaattaatgc actagtgaac agctcaaaat tttcataaat ttccttcgaa
    38161 tgctcaagcg gatgctgaac aaattctaaa gaaagcaaat aatccaccgt ctcttggctt
    38221 tccattaacg cttccaccag ctcattttgt ttctctaaaa aggtaaaata atgctgagta
    38281 gtttcctgat tagccggaat caaatataga aaatcctgat gaggacatag aacctcctcc
    38341 tttaccaact cgggaatgct aatttcttcg tcaatcaggc cattttaact caatatattg
    38401 cttccactcc gtctgagagc tatcaatcgg aggcgtggct gtcaaggaaa tcgtcaacag
    38461 attcggttgt tttttcttca agtcaatcaa caattcaccc cagctttttt ttaaatgatg
    38521 agcttcatca aaaaactaac agctccagct gatcaccata agttggctcc tccttcagtc
    38581 ggttgtaaat ggtctgatac gtttccactg taatatgact aggatgttca atcgccccag
    38641 aaagatagca actatctgct ggatttacta agaaatattc ctcgattgtt gttaaccact
    38701 gtttcttcaa tattagggaa ggaaccaaaa ttaaggtttt acaatccaaa cgtcggatca
    38761 tctcaattcc taaaaaggtc ttgccagccc ctggtggagc cactagatgg actgaattgt
    38821 tatttaaata agtagcaaag ttagtcaata tacgcttctg atagctacgc cactgaattt
    38881 tttcatcaga tagccacatc ccaggtcccc ctctcacgta ttttctctat cataatctaa
    38941 atgaatacag caaaaaaaag atttcttcaa gaatatgtgt ttttatagta atattttact
    39001 tactctaaac tcaaatgaaa aggtaaaaaa actggtagta caatctttta aattctcatt
    39061 gttttgtacg ttctttacta tcaaaaaaac aaaaaaaacg cgtttctacg cgtgaccaaa
    39121 atttttagct ctataatttc taagactgat agaaaatcat tctgtttttg aagtcttttt
    39181 tcataaatac tacttgattt attgtgtata ttgtataaaa gattgttcat aattattttt
    39241 tataattaaa atttggtatt gatgaccagc gattccgtat ataatgcgca atcaaattaa
    39301 gctttctgtc tctggtaaat attcctgttc tgttttcttg tactcgtaag atttcttcac
    39361 ttatcgcaaa attcataaaa ttccacactt tttcataaac aaaatgtaca catttatcta
    39421 ttacttcata atagtctact tgatattctt ctgtaaacat ggttgctact gtatctgcac
    39481 catatttgat aaacatttgg gctactccct taaattgaga aaagcccatt agtttatcta
    39541 ctaatacttc taaaatttct tgctttcttc tttaacatcg tcccgtaagg aatcatgtag
    39601 tatgctggag gaagttcatt ttctaaataa ttgtatcgct tcaattgctc aaaggctatt
    39661 ctctacaatt aattacacca attactttat cttcacttat ataagatttc taaacatttt
    39721 tcgtcaaatg aagagaaaat atttttttaa atgatcttgc tcaagatttg tcaacgaatc
    39781 tttgaattcg ttttgaagtt ctg
//
LOCUS       DAJSBM010000019        38086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJSBM010000019 DAJSBM010000000
VERSION     DAJSBM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.019"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..295)
                     /locus_tag="ONT67_001192"
     CDS             complement(<1..295)
                     /locus_tag="ONT67_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2354945.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYG"
     gene            complement(297..494)
                     /locus_tag="ONT67_001193"
     CDS             complement(297..494)
                     /locus_tag="ONT67_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCR2354946.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(888..2615)
                     /locus_tag="ONT67_001194"
     CDS             complement(888..2615)
                     /locus_tag="ONT67_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCR2354947.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2608..3801)
                     /locus_tag="ONT67_001195"
     CDS             complement(2608..3801)
                     /locus_tag="ONT67_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR2354948.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3988..4632)
                     /locus_tag="ONT67_001196"
     CDS             complement(3988..4632)
                     /locus_tag="ONT67_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2354949.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4726..4860)
                     /locus_tag="ONT67_001197"
     CDS             complement(4726..4860)
                     /locus_tag="ONT67_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCR2354950.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ONT67_001198"
     CDS             complement(4862..6994)
                     /gene="feoB"
                     /locus_tag="ONT67_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCR2354951.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6991..7464)
                     /locus_tag="ONT67_001199"
     CDS             complement(6991..7464)
                     /locus_tag="ONT67_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCR2354952.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7865..8089
                     /gene="nrdH"
                     /locus_tag="ONT67_001200"
     CDS             7865..8089
                     /gene="nrdH"
                     /locus_tag="ONT67_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR2354953.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8248..10407
                     /gene="nrdE"
                     /locus_tag="ONT67_001201"
     CDS             8248..10407
                     /gene="nrdE"
                     /locus_tag="ONT67_001201"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR2354954.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10457..11422
                     /gene="nrdF"
                     /locus_tag="ONT67_001202"
     CDS             10457..11422
                     /gene="nrdF"
                     /locus_tag="ONT67_001202"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR2354955.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11511..12650)
                     /locus_tag="ONT67_001203"
     CDS             complement(11511..12650)
                     /locus_tag="ONT67_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2354956.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12959..13672)
                     /locus_tag="ONT67_001204"
     CDS             complement(12959..13672)
                     /locus_tag="ONT67_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR2354957.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ONT67_001205"
     CDS             complement(13926..14627)
                     /gene="nagB"
                     /locus_tag="ONT67_001205"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR2354958.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14861..16393
                     /locus_tag="ONT67_001206"
     CDS             14861..16393
                     /locus_tag="ONT67_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2354959.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16584..17786)
                     /locus_tag="ONT67_001207"
     CDS             complement(16584..17786)
                     /locus_tag="ONT67_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2354960.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17868..18557)
                     /locus_tag="ONT67_001208"
     CDS             complement(17868..18557)
                     /locus_tag="ONT67_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2354961.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18663..19031)
                     /gene="rplL"
                     /locus_tag="ONT67_001209"
     CDS             complement(18663..19031)
                     /gene="rplL"
                     /locus_tag="ONT67_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR2354962.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19084..19587)
                     /locus_tag="ONT67_001210"
     CDS             complement(19084..19587)
                     /locus_tag="ONT67_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR2354963.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19791..19918)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19997..20686)
                     /gene="rplA"
                     /locus_tag="ONT67_001211"
     CDS             complement(19997..20686)
                     /gene="rplA"
                     /locus_tag="ONT67_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR2354964.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20790..21212)
                     /gene="rplK"
                     /locus_tag="ONT67_001212"
     CDS             complement(20790..21212)
                     /gene="rplK"
                     /locus_tag="ONT67_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR2354965.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            complement(21599..21838)
                     /locus_tag="ONT67_001213"
     CDS             complement(21599..21838)
                     /locus_tag="ONT67_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2354966.1"
                     /translation="MKKEYGYPTLKVICKASAGNETAIREILKFYDAYICKLCLRPFY
                     HSESGKITMQVDEELKGQIHTEMMKAILKFEIRVK"
     gene            complement(21843..22262)
                     /locus_tag="ONT67_001214"
     CDS             complement(21843..22262)
                     /locus_tag="ONT67_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008728841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2354967.1"
                     /translation="MNTSSFEHIVRIQFNALMITVIKCTVKSRNRQFARRSKREVLFY
                     ELSDTKNLECVTEDKYSCDYISFEVLNFTIQISNEKLAVALYKLSSKERDVILLRYFQ
                     SMSDQEIAELYHVSRSAIYRRRSNGLKKLKTLLKERN"
     gene            complement(22503..22907)
                     /locus_tag="ONT67_001215"
     CDS             complement(22503..22907)
                     /locus_tag="ONT67_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HCR2354968.1"
                     /translation="MKKPKVAFVCVHNSCRSQIAEALGKYLSSNVFDSYSAGTETKPQ
                     INQDAVRLMKEMYQIDMEKTQYSKLLSEIPQVDIVITMGCNVNCPTIPCKYREDWGLN
                     DPTGKNDDEFLKTIHSIHAKILELTEKIKIMR"
     gene            complement(22937..23269)
                     /locus_tag="ONT67_001216"
     CDS             complement(22937..23269)
                     /locus_tag="ONT67_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2354969.1"
                     /translation="MKLFGKKKETKSCCCGGNCTSETMQNAENKKQEQGIKILGSGCA
                     KCMELEKATRTAISELQLDYEIEHITDFAEIASYGVMSTPALVFNGEVISYGKVLTVD
                     EVKALLSK"
     gene            complement(23286..24281)
                     /locus_tag="ONT67_001217"
     CDS             complement(23286..24281)
                     /locus_tag="ONT67_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR2354970.1"
                     /translation="MWLFFQDQILGMKWLNGLIGNGLSLLGLDINSRLGGSIQFFLYD
                     VIKITILLCLLIFFISYIQSYFPPERSKKILGRFHGIGANIISALLGTVTPFCSCSSI
                     PLFIGFTSAGLPLGVTFSFLISSPMVDLGSLVLLMSIFGAKVAFAYVIVGLIIAVIGG
                     TLIEKMHMEKYVEDFVKNASNVDISSPTLTKKDRVQYAKEQVVGTFKKVFPYILIGVG
                     IGAVIHNWIPETWIESILGSNNPFGVILATLVGVPMYADIFGTIPVAEALLSKGAQLG
                     TILSFMMAVTTLSLPSLIMLKKAVKPKLLTLFIAICTFGIIIVGYLFNIFSTLFI"
     gene            complement(24377..24682)
                     /locus_tag="ONT67_001218"
     CDS             complement(24377..24682)
                     /locus_tag="ONT67_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354971.1"
                     /translation="MNDFSKDAKIFKALCDTKRLTILDYLKSGEKCACVLIENMNIGQ
                     SALSYHMKILCDSGIVNARQEGKWTHYSLSKSGSEYASKRLLELTTPNAENQSICCK"
     gene            complement(24910..25545)
                     /locus_tag="ONT67_001219"
     CDS             complement(24910..25545)
                     /locus_tag="ONT67_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2354972.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRTEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGTNIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYRTPYQVVKMINPNSKEDMELLREVLRNNHFDDEDE"
     gene            26055..27197
                     /locus_tag="ONT67_001220"
     CDS             26055..27197
                     /locus_tag="ONT67_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2354973.1"
                     /translation="MNNLEFALEMKNKRLASGLSQRELAGLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRLPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGTGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGAECRCKNYENVQSGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            27190..27396
                     /locus_tag="ONT67_001221"
     CDS             27190..27396
                     /locus_tag="ONT67_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2354974.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            27475..28668
                     /locus_tag="ONT67_001222"
     CDS             27475..28668
                     /locus_tag="ONT67_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2354975.1"
                     /translation="MREKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKRKKPSLRELEQQIRRDIEDGIDSAGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDMLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKNKEQGYYIETPKTKSGIRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGRPKVAIDYNALFVRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASRNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            28984..29652
                     /gene="sdaAB"
                     /locus_tag="ONT67_001223"
     CDS             28984..29652
                     /gene="sdaAB"
                     /locus_tag="ONT67_001223"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCR2354976.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            29665..30537
                     /gene="sdaAA"
                     /locus_tag="ONT67_001224"
     CDS             29665..30537
                     /gene="sdaAA"
                     /locus_tag="ONT67_001224"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCR2354977.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(30628..31647)
                     /locus_tag="ONT67_001225"
     CDS             complement(30628..31647)
                     /locus_tag="ONT67_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCR2354978.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(31820..32725)
                     /locus_tag="ONT67_001226"
     CDS             complement(31820..32725)
                     /locus_tag="ONT67_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2354979.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(32784..33329)
                     /gene="nusG"
                     /locus_tag="ONT67_001227"
     CDS             complement(32784..33329)
                     /gene="nusG"
                     /locus_tag="ONT67_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR2354980.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(33430..33600)
                     /gene="secE"
                     /locus_tag="ONT67_001228"
     CDS             complement(33430..33600)
                     /gene="secE"
                     /locus_tag="ONT67_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR2354981.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(33624..33776)
                     /gene="rpmG"
                     /locus_tag="ONT67_001229"
     CDS             complement(33624..33776)
                     /gene="rpmG"
                     /locus_tag="ONT67_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2354982.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            33938..34582
                     /locus_tag="ONT67_001230"
     CDS             33938..34582
                     /locus_tag="ONT67_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2354983.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(34638..35141)
                     /locus_tag="ONT67_001231"
     CDS             complement(34638..35141)
                     /locus_tag="ONT67_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2354984.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(35154..35200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            35738..36703
                     /locus_tag="ONT67_001232"
     CDS             35738..36703
                     /locus_tag="ONT67_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCR2354985.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(36802..37974)
                     /locus_tag="ONT67_001233"
     CDS             complement(36802..37974)
                     /locus_tag="ONT67_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354986.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(37961..>38086)
                     /locus_tag="ONT67_001234"
     CDS             complement(37961..>38086)
                     /locus_tag="ONT67_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2354987.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        11796 a         7723 c         6691 g        11876 t
ORIGIN      
        1 ctccataatc tgctcgatat aaaccattta ttccagcagt tcttccaatt tcactcaaat
       61 ttcttattaa ttcctcttga tattcagtga atcttatttt attagtataa gcagtaaaat
      121 atagataatt caccaatcta tttattaaag gaccgggatc taattcatat ttttgttttt
      181 ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta ggatctttac
      241 ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt ttcattttag
      301 cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc aatacaagta
      361 gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc atttccataa
      421 tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat agacaaaatt
      481 tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat tattctaaca
      541 ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt cctgaataag
      601 aatttaatca cttttaagta tataaactat gtatattctt taatattttt tgagaatgca
      661 cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg atctctttga
      721 ctcgcaccca taaattcata atagtttgtc ggcgtctttg gacgtttcga agatgagcta
      781 cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt ccaaatttgg
      841 atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta ttttttccag
      901 ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg tggatctttt
      961 ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg gaaattgtct
     1021 aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat cgtatggatc
     1081 tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc atatttggca
     1141 aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt gatcgcttga
     1201 cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc tttcaagttg
     1261 tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg ctcgattcct
     1321 atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc catgtaggct
     1381 ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc gacttgatct
     1441 ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac catggcatct
     1501 gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt catcgttaca
     1561 tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac ttctgtatcc
     1621 cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa actccctata
     1681 ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg acgtttttta
     1741 cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa atgctgtgta
     1801 aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa tccattcccg
     1861 acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc accgatcata
     1921 gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc tgccatgatc
     1981 gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt gctcccgaat
     2041 gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt cgtaaaacga
     2101 acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat cccgataccg
     2161 aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat aaagtcaagg
     2221 atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc taatggtacc
     2281 ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt catcaaatca
     2341 ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa cgtaagtcct
     2401 ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa caaaggagct
     2461 ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat cgtttgtaaa
     2521 aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc tacccagttt
     2581 gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc ttgtttctgc
     2641 taatgcttca atcacactac ctcggatgac gacacctttt aattttccat cctctacaac
     2701 tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca ccatgtcttt
     2761 cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt gcttcctagc
     2821 ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt caacagccat
     2881 cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc catcagattc
     2941 gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt tggaacgatc
     3001 gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata tttcttcacc
     3061 tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc caatccgtaa
     3121 agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg cttgcaggtc
     3181 cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga acgcttcgtc
     3241 catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc gttgttgcat
     3301 cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag aattttctaa
     3361 tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt ctaacccgta
     3421 ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga aaaccatatt
     3481 gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata catcttctcc
     3541 atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac ggatcaatgt
     3601 ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt ttacgtcaaa
     3661 attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg ttttcggctg
     3721 gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct ttgttaaatg
     3781 ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat ttttctttct
     3841 gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa tgaaagaaat
     3901 ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg tttagccaag
     3961 ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg ggtaaaaaaa
     4021 gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt cccctgccgt
     4081 aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa ttgcgataac
     4141 tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca gatgcattgc
     4201 tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta ttcgcttcgt
     4261 ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt gttgcttctc
     4321 cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt gatattgcaa
     4381 taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat gccgtacttc
     4441 catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag gagaagcatt
     4501 gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat atcggttttc
     4561 aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga cgattttttt
     4621 ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta ctatgctcta
     4681 gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc gcttgatctg
     4741 ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg attttaaacg
     4801 agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga atgtcgccat
     4861 cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat tttggctgac
     4921 ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc cctaacgcta
     4981 cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact aaataagcta
     5041 aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt tcccgatgga
     5101 tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac aatgaataag
     5161 ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca ttttcggata
     5221 cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta gcgaccaatc
     5281 ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga aaaacaactg
     5341 ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt tgagcataaa
     5401 aattataaga agagctgaac cagatcacga tactagaaac aaagataatc gttcctgctt
     5461 ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac cattgaggaa
     5521 aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac aaacgtgttt
     5581 ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag gcagaaggag
     5641 ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc ggcaactttg
     5701 cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc tcgttttcaa
     5761 tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc ataggaatga
     5821 aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa gcgatcctcg
     5881 ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc aattgcggta
     5941 gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc tcctgcatcc
     6001 atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac catgtaccaa
     6061 acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata gccagataat
     6121 agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc gttgcaatct
     6181 gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca cagagtgtac
     6241 atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta tcgtcgtcca
     6301 atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa actgctatct
     6361 cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac cagcgtttct
     6421 tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct aaacgtggat
     6481 catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga atcttttcga
     6541 ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt acaccaagac
     6601 cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca tccatcatat
     6661 tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa taaagatttc
     6721 gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc tgaaccaaat
     6781 aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg ccgggtaggt
     6841 cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct actgttacgc
     6901 ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag ctagttttcc
     6961 cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt gtatcaagat
     7021 tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca gttcgattgg
     7081 atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca atcccatatc
     7141 cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc cagtctctcc
     7201 tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt tattgacaaa
     7261 gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca gtaaaatggc
     7321 attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc cgatattttg
     7381 caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa cgaaattctg
     7441 ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat catgtattaa
     7501 aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga gaatcattct
     7561 cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt atgagcgaat
     7621 ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt ccacaatttt
     7681 ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt ttttcaaaaa
     7741 cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt tgtttgactt
     7801 ttttactaag agttttatac tatctattat agataataag aatgtgttag aggaggcact
     7861 caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga tggtcaaacg
     7921 gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac agcctgaagc
     7981 agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt ctgatgcaga
     8041 aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac atgactaaaa
     8101 gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt ttgtcgcaaa
     8161 cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca agcatcatca
     8221 aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga tgtcagttat
     8281 ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt gaataaggat
     8341 aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat gacttttcta
     8401 tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc agaattcatt
     8461 caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga gcaacatttt
     8521 tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt gaaaaccaac
     8581 gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc gctttacttt
     8641 gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca tcaacgatat
     8701 cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga attggtgtct
     8761 tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat caactctgct
     8821 ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct tcgtgaagcc
     8881 ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt catgaaatta
     8941 ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc tggcgtggtt
     9001 tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa agaaaatgca
     9061 gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga taaattttat
     9121 gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt cgaaaaagaa
     9181 tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct agttgcaaat
     9241 ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat ctcaaaatta
     9301 caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg cgagaatcca
     9361 atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt tcaaacgcct
     9421 tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cggatatctc atgtaactta
     9481 ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc tgtgcgtacg
     9541 atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt accaccgatc
     9601 caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt acacacttat
     9661 cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac aaatatttac
     9721 ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg tgaaagaaaa
     9781 caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt cgaaaaatac
     9841 gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga aggcattttt
     9901 atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga tggactgtat
     9961 catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa cgatacgagt
    10021 gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa aatcggtaaa
    10081 atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac ttctgcttat
    10141 gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca cgtcgatcaa
    10201 ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta tgaatggaag
    10261 acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc tttccacaaa
    10321 ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga aatcggaagc
    10381 aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt catattttaa
    10441 ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga atgaaattga
    10501 agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc tagatacacg
    10561 tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg aaaaagatac
    10621 agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg aaagtggcat
    10681 ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga acaatattca
    10741 attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac tgaatacgaa
    10801 aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc aaaaaaaagc
    10861 tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa ttgccagcgt
    10921 ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt atttaggaaa
    10981 caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg aatctgtaca
    11041 tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg aagcagaaca
    11101 agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa acgaagaacg
    11161 ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga cattcctacg
    11221 ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc cagatactgc
    11281 caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca accatgactt
    11341 cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga aagacgacga
    11401 ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg aaaaataaaa
    11461 agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc ttattcactt
    11521 gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat tcttctcaat
    11581 aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac aggtatgcaa
    11641 atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa gatgatcgtc
    11701 aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt tccatctaat
    11761 tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga gataaacaaa
    11821 ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat aatattcgtg
    11881 attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct cattgccaaa
    11941 aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata ggcagataat
    12001 gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt cggaaagaag
    12061 tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa aataaatgga
    12121 acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga gacacttgca
    12181 gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc tgatgctaat
    12241 tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt taaccatttg
    12301 cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg ttccattaac
    12361 tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa aaacgtcaat
    12421 aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat tggatcgaac
    12481 atataataga taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag cattttcaat
    12541 attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat cgtgagtact
    12601 agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat acatctcctc
    12661 caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct gagattttca
    12721 tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag tacttaaaca
    12781 aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc aaataaacgg
    12841 tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata aggaactatt
    12901 tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct cagatcattt
    12961 attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac aactcctcga
    13021 tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt ttgtacactc
    13081 ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata tttttctctt
    13141 caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg atttctaact
    13201 ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata gcaatcccga
    13261 taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct aatccagcaa
    13321 ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg acgatgatcg
    13381 ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt gcttcatctg
    13441 aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca ccactagcga
    13501 tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata tgcgtacgca
    13561 tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca aatagcatag
    13621 cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc atctaaacac
    13681 cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa tttttacctc
    13741 ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa taaacggtgt
    13801 tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag ctatttttcc
    13861 tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta gcctactatt
    13921 gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt gtcgtgattt
    13981 tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc tttgattgca
    14041 tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat aattgaaccg
    14101 attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa tcgtttgttt
    14161 gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa tgaagcacca
    14221 ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat atcgattgga
    14281 tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc tgcttttcca
    14341 tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc catgaaatga
    14401 cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt tacagaagtc
    14461 atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac tggtgtgctt
    14521 cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat gatttcaaat
    14581 gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt gattgctcct
    14641 ttatattttg gtatagccca attatagcat tctttttttc tttttacaat aattagtata
    14701 gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt taggtttcat
    14761 aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat tcactgtgga
    14821 cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac tgacattaaa
    14881 caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc tgcaattaaa
    14941 tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta cattgattaa
    15001 aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa aaaatattca
    15061 caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg aagaatttct
    15121 aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc tttaccaaac
    15181 atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc aaacggaatt
    15241 agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca gcgggttggg
    15301 gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac aacgttcaaa
    15361 ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag atgaaccaac
    15421 caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg attttgacgg
    15481 tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca cccatatcgc
    15541 agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag cattgaaaca
    15601 aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa aacaaatcga
    15661 aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga tggcaaaaag
    15721 ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag caagtaaagc
    15781 tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa caactgattt
    15841 agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc gctttggtga
    15901 aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa aaacactgat
    15961 cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa aaatcaatta
    16021 cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat atttaagcag
    16081 tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag cagggttagt
    16141 taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa ctaaggtgaa
    16201 acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac cgacgaacca
    16261 tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc caggaacagt
    16321 aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag tcattgagat
    16381 tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac aaaagttttg
    16441 tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt attagctcaa
    16501 aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt gatgcagaac
    16561 agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt tcctttaaaa
    16621 acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac cgttacacct
    16681 aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc acctccaata
    16741 aagtacataa acataaatac agtattattt cggttgcttg ctgcttcacc tagcccttgt
    16801 actcgtgttt gattggcgac ttgaccaaat tgattcccga cgtctaataa aataatagct
    16861 aaaagcaaaa tggccaaatg gcttccgcca accaataaag caataaagct tattgtttgc
    16921 ataacaagtc ccatatacac gatttttctt tcagaataag aatctgccag tcgtcccacg
    16981 ataggtgcag caactgctcc agacaaacca aaaatcgcta atatccctgc ttcgaatgtt
    17041 ccccaattgt aggcgggact gctgatataa aaaatcaacg tagaccaaaa gatcgaaaat
    17101 gttccaaaca tgaaaaatcc actgactgcc gcttctctta atagacgttg agaagcaact
    17161 aaatgcggca aacttttaag tgaatcaagg tatgaaacag ctgtactact ttgctggaca
    17221 gaacgcggca ttttccagta aagcagaagt gtaagaattc cgactgcaca taaagcaaat
    17281 aaatagatca tccgccaaga aaaaagactg gcaacaatac cagataccgt ccgagaaagc
    17341 aaaatcccca cgagtaaacc gctaagcaat gtacccatga ccttccctct tgcttttggt
    17401 cccgctagta ccgcaccgta aggaatgatg atttgcggca cgatcgaaag taatcctatt
    17461 aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc caacagagag
    17521 acggcagata acgctgccat tcgaatgatc aattttggac gatccaccac gtcccctaaa
    17581 ggaactaaaa acagtaaccc taacgcatag cctaattggg tcaacatcgt caatatcccg
    17641 actgcacttg tgctaacaga taaactagct gctacttttg ttccaatcgg ctggatgtaa
    17701 tacatattgg caacaacaac gccacaagtg attgataata gtaaaataag cgaagttgtt
    17761 aaaggttttt tttcattact cttcattgat ttcccccatt tcctatctta gtttacaact
    17821 tatggaaaag attggaaata gaaaaataca actattcgcc acacttatta tttgtgcttt
    17881 tgttttcctt gttttttctt gcgatcttta taaatgaaga caactaaccc tactagcaca
    17941 gtaacacttg caaccaacgt gactgcagtt ttgttatatc cgtgaaacag agcttctcca
    18001 ccacacgcat acaacacaga cgaagggatg actccaatca cgatcgacaa tatgatttgc
    18061 tgtagaggca atttcatcgc atctgcagca aaattgacca tagaagatgg aataaccggt
    18121 atcatatagc ctaacattac acctattttt ggatgtttca tttgtcggat tgcttgcacc
    18181 caatgattcg tttcggtttt cttatctaaa aattttaagc gatgcataag gaaaattgca
    18241 gtgatgttac caagagcatt acccaaaatg ttgatgatcg cacctgtcag tggtccaaag
    18301 ctcaaaccag caatgactcc aactactgaa gtaggaatac ctggaacagc acacatcacc
    18361 gctgtcaata cgatcaagag tatcgatgca aaagtcccat gcgaacgaat atgtgcgagt
    18421 agctctgcct tactcgctct cggatttaaa aataattgga tgtctgtctg atattctaaa
    18481 tacaaccgat agcccactac cagtatcaga atgattccca acgagatcaa tatcaatcgg
    18541 attattttct ttttcatatt tcctcctgaa aaaaatctat attcattaaa taatagttta
    18601 acataaaaac aaaaaagcac cgaaatccaa cataggatct cagtgctttt aaatagtaaa
    18661 aattatttaa cagttacaga tgcgccaact tcttcaagtt tagctttcaa ttcttcagct
    18721 tcttctttag atacgccttc tttaactggt gcaggagcgc catcaactac agctttagct
    18781 tctttcaagc ctaaaccagt tgcttcacga actgctttga taactttaac tttttggtct
    18841 ccagctgcag ttaattctac agtgaattct gtttgttctt cagcagcagc agcgccacca
    18901 gcagcagcta cagctacagg agcagcagca gatacgccaa attcttcttc aatagcttta
    18961 actaggtcgt taagttctag gatagttgct tcttttaact cagcaacgat gttttcaatg
    19021 ttcaatgcca ttataatttc ctccgtttaa ttgttctttt ttatttaatg ctttatgcgg
    19081 caattaagct gcatctccat cttctttgtc tgccactgct ttgacagcgt aagccacgtt
    19141 gcggactggc gcttgtagta cagatagcaa catagataga agtccttcgc gatttggcaa
    19201 ttttgccaat gctgtgattt cttccaatga agagacttta ccttcgatga taccgccttt
    19261 gatttctaat gcttttgctt ctttagcaaa ttcgtccatg attttcgctg gtgcgactac
    19321 atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca tgccttcaag
    19381 gccagctttt ttagctgcac gtgaaagaat agaatttttg ataactttca tttctacgtt
    19441 agcatcacga agttgtttac gtaaacgtgt tacttcatca actgttaatc cgcggtagtc
    19501 aacgactact actgatgcag ctgctgtaaa tttttcgctt acttcatcaa cgattgctgc
    19561 tttttttgcg ataattgctt cactcatttt tgagtttcac ctcctgaatt ttgatggaag
    19621 agttttttgc actgaaaagg caagtcactt ttatagttac tttgctcaac cagtattatt
    19681 catcacctat tcacttcgtt catagtgatt ctactcgctt tgctcgaaag gaaactcctt
    19741 tcgctttgct caaccagtat tgttcatcat ctcttcactt cgttcatagt gattcacaat
    19801 aaaaaactct atgccaccgc agacatagag ggactattga atatattcaa taaacgtcct
    19861 cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa ttattaacct
    19921 caactagctt agcatgctaa actagttgag tcaagaatta tttttcagtt ggatcaatca
    19981 acattctaat atttaattag aaagaagctt ggtcaacatg gataccaggt ccaaatgttg
    20041 ttgtgatcgt tacatttttc atgtattgac ctttagctgc tgatggttta gcttttagca
    20101 atacatcatg gattgctgca aagttttcaa ctaatttttc atcgtcaaat gaaacttttc
    20161 cgattggtac atggatattt ccagctttgt ccacacggta agttacttta ccagctttca
    20221 cttcgtttac tgcttttgtt acgtccattg taactgtacc agttttaggg tttggcatta
    20281 agccttttgg tcctaatact cgtcctagac gacctacagt agccatcata tcaggtgttg
    20341 caacaactac atcaaagtca aaccatccgt tttggatttt ttgaaccatg tcgtcgtcac
    20401 ctacgaaatc tgcaccagct gcttcagctt cttttgcttt gtctccttta gcaaaaacta
    20461 aaacagtttg tgttttacca gttccgtttg gtaatacaac agcaccacgg atttgttggt
    20521 ctgctttttt agggtctacg ttcaaacggt atgcaacttc aactgttgca tcaaattttg
    20581 caatgtttgt ttcttttgct aaagctactg cttcggcaac aggataaact ttacttgaat
    20641 caactttttt taatgcatct tgcatttttt tgctcttttt agccattttg gttcctcctt
    20701 gattgtggtt ataacggttg tacctcccac gtgttctata tctttcaata tagagccaac
    20761 tgagaagctg ctgtcttagg gtgtatagat tattctacag tgatccccat gcttcgtgca
    20821 gttccttcaa ccatgcgcat tgctgcttca acgttagctg cgtttaggtc ttccattttt
    20881 aattcagcga tttctcttac ttgatcgcta gaaactttcg caactttatt tttgtttggt
    20941 tcgcctgaac ctttttcgat tttagctgct tttttcaata gaacagcagc tggcggtgtt
    21001 tttgtaacga atgtgaatga acggtcttcg tatacagaaa taacaaccgg aatgatcaaa
    21061 cctgcttgat cagctgtacg agcgttgaat tcttttgtga atcccatgat attgataccc
    21121 gcttgaccta gtgcaggacc tactggtgga gctggagttg ctttacctgc aggaatttgc
    21181 aatttaacga ttttttctac tttctttgcc acgagacata cctccttaag tccgtgatgt
    21241 ggttaattgg agatgcatat ttctcctccc acgttttatc tgatctgaaa aaatccagac
    21301 aaataatgtg caaaacaatg cacactggta cagtatagca aatttatttg ttttttcaag
    21361 atttttttct gctattttta cattttgctt gaataaacgc taaatttaat ataaatttta
    21421 tatctcattt tcaattatcc gcataaatac tatacattca agtattttaa ggtatagaaa
    21481 aacactcatt ttaccacgaa tttaccacga ttgaatgagc cttgatatga cacaaaaaac
    21541 aacatgggag atagcacaaa tatctgtgta tctcccgttt ttcattcatg taatatgatt
    21601 actttactct gatttcaaat ttcaagattg ctttcatcat ttctgtatga atttgacctt
    21661 ttaattcttc atcaacctgc atagtaattt taccactttc agagtggtaa aacggacgca
    21721 agcataattt acaaatataa gcgtcataaa acttcaaaat ctctcttatg gcagtttcat
    21781 ttccagcaga cgctttacat attactttta atgtagggta gccatattct tttttcattg
    21841 tttcaattcc tttctttcaa taatgttttt aatttcttca atccgttact tcttctgcga
    21901 taaatagcag aacgtgatac atgatataat tctgcaattt cttggtcgct catgctttga
    21961 aaatagcgta atagaattac atcacgctct ttactcgata acttatataa agcaacagca
    22021 agtttctcgt ttgatatttg aattgtgaaa ttcaagactt caaaagaaat ataatcacaa
    22081 gaatatttat cctcagttac acattcaaga tttttcgtat ctgataattc atagaataaa
    22141 acctcacgct tggaacgtct tgcaaactgt ctatttctgc tttttactgt gcatttaata
    22201 acagttatca tcaaagcatt aaactgtatt ctgacgatat gctcgaaaga agatgtgttc
    22261 ataatctcac cccctttctg catacgccga aatatataaa cgggaatacc gcctatacct
    22321 tttggtaatg taaaaaagaa agcaagaaaa aagcccgcac aaaacatata gtctgcacga
    22381 gccttcaaaa tttccttata ttcagatgtt gataattgcc atattagact gtttaatagt
    22441 gtgcttaaaa caatttatga caatattctt cgttaatatt ttcatttagt ttctccttga
    22501 aatcaacgca ttatttttat tttttctgtc aattcaagta tttttgcatg gattgaatgg
    22561 attgtcttta aaaattcatc atcatttttc cctgtagggt cattcaaccc ccaatcctct
    22621 ctatatttgc atggtattgt aggacaattc acattgcacc ccattgtaat cacaatatct
    22681 acctgtggta tttcagataa caatttagaa tattgtgttt tctccatatc tatctgatac
    22741 atttctttca ttagacgaac tgcatcttga ttgatttgtg gctttgtttc tgtgcctgcg
    22801 gaataactat caaaaacatt tgaagaaaga tacttgccca atgcctccgc aatttgactg
    22861 cgacaagaat tatgaacaca gacaaaggct actttaggtt ttttcataaa tgaattacca
    22921 acctttcttt gttttattat ttgctcaata atgcttttac ttcgtctacg gtcaatactt
    22981 ttccatagga tattacttcg ccattaaaca ccaatgcagg tgtagacatt actccataac
    23041 tagcgatttc tgcaaaatct gtaatgtgtt caatctcata atctaattga agttccgata
    23101 ttgctgttct agttgctttt tccaattcca tacattttgc acagcccgaa cctaatattt
    23161 taatgccttg ctcttgtttc ttgttttctg cattttgcat tgtttctgat gtgcaatttc
    23221 caccacaaca acatgatttt gtttcttttt tctttccaaa taatttcatt tcttattcgc
    23281 tccttttaaa taaataatgt actaaaaata ttaaagagat aacctacaat aataatgcca
    23341 aacgtacaaa tggcaataaa aagggtcaat agtttaggct ttactgcctt tttcagcatt
    23401 attaaagacg gcaaactcaa tgttgttaca gccatcataa atgataaaat tgtacctaat
    23461 tgtgcaccct ttgaaagtaa tgcttctgct actggaattg ttccaaaaat atcagcatac
    23521 ataggaactc cgacaagagt ggctaaaata acaccaaacg ggttattact gcccaagata
    23581 ctttcaatcc atgtttcagg tatccagtta tggataactg ctccgatacc cacaccaatc
    23641 aaaatgtatg gaaatacttt cttgaatgtt cctacaactt gttcttttgc atattgaact
    23701 ctatcctttt tggttaaagt aggagaacta atatcgacat tacttgcatt tttcacgaaa
    23761 tcttccacat atttttccat gtgcattttt tcaattaaag taccgccaat tacagcgatt
    23821 atcaagccta caatcacata agcaaaagca actttagccc cgaaaatact cattagcaat
    23881 acaagactcc ctaagtcaac cataggcgaa gaaatcaaaa aggaaaaagt tactcctaac
    23941 ggtaatcctg cacttgtaaa acctataaac aacgggatag aagaacacga gcaaaatggt
    24001 gtaactgttc ctaacaatgc ggatatgata tttgcaccga taccatgaaa tcgccctaaa
    24061 atttttttac ttcgttcggg tggaaaataa ctttgaatat acgatataaa gaaaatcagt
    24121 aaacataaca agattgtgat tttgattaca tcgtataaaa agaattgaat acttccacct
    24181 aaacgactgt taatgtctag ccctagcaat gatagcccat taccaattaa gccattcaac
    24241 catttcatac ctaatatttg gtcttgaaaa aatagccata ttgaatttac tacttccaat
    24301 aaagtccctc ctttacatga catatcaatt ttttttgatg tgataaagtt ttataaaagc
    24361 cacctttcga tgacctttat ttgcaacaaa ttgattgatt ttcagcattt ggtgttgtta
    24421 gttctaacaa tcgttttgac gcatattcac ttccactttt acttaggctg taatgagtcc
    24481 attttccctc ttgtcttgca ttaacaatac cagagtcgca aagtattttc atgtggtagg
    24541 ataaagccga ttgtcctatg ttcatgtttt caatcagaac acacgcacat ttttctccgc
    24601 tctttaagta atctaaaatc gtaagtcttt ttgtatcaca aagagcctta aaaatttttg
    24661 cgtctttaga aaaatcattc attattatca ctcctcacat caaataattt tgatatattt
    24721 atcataaggc ttacaacatg atttgtcaac acattacatc aaaatatttt gatgtattat
    24781 tcttttttcc tcttgattac ccataacaga aaagaaaaag ccctgtcaag agcttgccac
    24841 cattggtggt gctttgcact ctttactggg ctttttgttt gctgtatctt ccatgcaaga
    24901 ggaaaaagat tattcgtcct catcatcaaa atggttattc cttaatactt cccgtaataa
    24961 ttccatatct tcttttgaat tagggtttat cattttgacc acttgataag gtgtcctata
    25021 tttatgtccc tctatgcttt ccccaaacat atccatgctg tataaatcat catagcggtc
    25081 taacagtttt tgaaacttca aatgctggat aaattctttg attgggtaaa ggtcggacgc
    25141 ttcaatgttt gttccattga tgtagtcggt aatatattct cgtaactttt ctaattcata
    25201 ttccagccat tcttcctcgc tgtcaaagaa gttgtcataa tgtccatgtt ttaattctgc
    25261 atttagacgt tcttctgttc ttaaaaactg acagacttct ttttcggact gattgacata
    25321 tttccattcc cccgacaaca attcattcgg cgtaacgtcc agcacttcca ttatcttttc
    25381 cagcgtttca taagcaggat agttcaaccc tctttcaatc ttggaaaggc tctgcatatt
    25441 gataccaatt ttgtccgcaa gttcctgttg tttcattccc ctaaattttc ttatggtctg
    25501 tatattattt cctaaatggc ttattttcat tgcataaccc tccataactc gttatatctt
    25561 cgctgctata agtctatcat gcttaatatg atttgtaaag ataaaaaaag atattgacaa
    25621 ataagcatat aagaattact atgttcttgt aatcaaataa gcacaataga atgaaataca
    25681 aaaacattca aaagaaaata tctatctgat taaacccgta gatatgaata tcggttgaaa
    25741 tggaacttgt ttgaaaggac aaacacagga cttgccgaca gagcattgta ggactgttgg
    25801 cgtgcgtcag caacaacgcc atgacggctt gccgtcaaag gacggaaatg ctttgtcggc
    25861 tggcgaactt gtgagccttg tctgtgcccc cgttatctaa caggggggca ctatttcaca
    25921 aaagacaagt caaaaaccat acaaccccaa agctgtaagg agtaatgagg agttttaaga
    25981 agatagtaac ttcacattct tttgaatagt ggtttacagg tatttcaagg ctgttgtggg
    26041 attggaggaa tacaatgaac aatttagaat ttgcacttga aatgaaaaac aaacgtcttg
    26101 caagcggtct ttcacaaaga gaacttgcag gtcttaccca tgtatcaaga tatagtatca
    26161 accgctttga gaacggaaaa gccaacgcca gcaaggaaac acaaaatatt atcctgcgtt
    26221 gtctgaatta ctatatctgc gataagcctt tttatctgct cgttgattat ctgtctgtcc
    26281 gtttaccaac gactgacgca ttagaagtta tccgaaaggt gcttggcatg aaagcagatt
    26341 attttatcca ttatgactat gggtattatg gctataagga gcattacgcc tacggggaaa
    26401 tcaaggtcat ggcttctgat gatgaacaca tgggcgtatt tttggaatta aaagggacag
    26461 gctcacgcaa catggaatat gtcttacagg cacagaacag ggattggtat tcatttttaa
    26521 accgctgtct tgactgtggc ggtgttatcc gacgttttga tttggcaatc aatgatatgt
    26581 gcggtctgct ggatattccc gttttgtctg aaaaatataa aaatggcggt gcggaatgtc
    26641 gctgtaaaaa ctatgaaaat gtacaaagtg gaaaattaag tgggaaaaac cgcaatttag
    26701 caagtaccct ttatatcggt tcaaaggcaa gcacaaaata tttctgcctg tatgaaaaac
    26761 aaaaggaaca ggcaacgaaa aagaaacata ccgatattat caaccgtttt gaaattcgtc
    26821 tgcgtgataa aaaggcagta caggcagttg aggaattgtt attgacatat aaccctcatg
    26881 ggctggtgtt ttacctcatt actgattttg tgcaatttcc cgactatccg ttgtgggaga
    26941 tattcatttc ccatgacagt ttaccttttg aaatgaaccc cgtacctgtc aatatggaac
    27001 gcactctgca atggctggaa agacaggtca tgccgtcaat cgtgatgata gaggaaatcg
    27061 acaggctgac aggctcaaac tacatgaaaa tgattgatga atgtacccac ctttccgaaa
    27121 aacaggaaat gcttgtagaa cagatgtgta cggatatagc agatgtaatc gaaagtgagg
    27181 gggtgtttta tgagtaatgc acaggatatt cccgtatggg aaaaatatac ccttaccatt
    27241 gaagaagcgt caaagtattt ccgtatcgga gaaaacaagt taagacgatt ggcagaggaa
    27301 aacaaggacg ctggctggct cattatgaat ggcaaccgca tacagattaa acgccgacag
    27361 tttgaacagg ttattgataa attggacgca atctaatgca aatgagcctt gtatgtgtta
    27421 tgataagcac aagtcatatc aaggctcttt ccaacaagga aaggagcaga caccatgaga
    27481 gaaaaaagac gggataacaa aggtcgtatc ctgcatactg gagagagcca acgaacagac
    27541 ggaaaatact tatataaata tgtagacgca tttggaaaca caaaatatgt gtatgcttgg
    27601 agattgacac ccacagaccc gacaccaaag ggaaaacgga aaaaaccctc acttcgagaa
    27661 ctggaacagc agataagacg ggatattgag gacggtatcg acagcgcagg caagaaaatg
    27721 acgctttgcc aactctatgc caaacagaac gcacaaaggg caaatgtgaa gaaaagcaca
    27781 caaaaacaac gggaacaact catgcggtta ttgaaagagg acaaattagg tgctaggagc
    27841 attgatacaa tcaagccctc tgacgctaaa gaatgggcgt tacgcatgaa agacaagggc
    27901 ttttcctaca ataccattaa caaccataaa cgctcgttaa aagcgtcatt ctatatcgcc
    27961 atacaagacg attgcgtaag gaaaaatccc tttgatttca aattaagtga agtcctagaa
    28021 aatgatacca aagagaaagt cgcattgaca gaggaacagg aacaagcctt actctcattc
    28081 atcaagacgg acaatgtgta tcacaaatat tatgatgata tgctgatatt gttaaaaaca
    28141 ggacttcgta tctcggaact gtgcggactg acagtagccg atattgattt caagaatgaa
    28201 gttgtgatta tcgaccacca gttattaaag aacaaggaac agggctatta tattgaaacg
    28261 cctaaaacaa agagcggaat aaggcaagtg ccattaagca gagaaacgat acaggcattt
    28321 caacgggtta tgaaaaaacg cccaaaggca gaaccatttg tgatagacgg acggagcaat
    28381 tttctgtttg tcaatcataa gggtaggccc aaagttgcga ttgattacaa cgccttattt
    28441 gtccgaatgg taaagaaata caacaagcac cacaaggaca atcccttgcc acatatcaca
    28501 ccgcatacgc tacgccatac attctgcaca aggctggcaa gcaggaacat gaacccgaaa
    28561 gatttacagt atatcatggg acattcaaac atcagtatca caatgaactg gtatgctcat
    28621 gcgtccatag ataccgcaaa atcagaggtt cagcgtctaa tcgcataaga agtatttacc
    28681 acgattttaa ccacgattga cagggaaaat ataagaagat agatctagat atgtgaggtt
    28741 taccacaaaa acaaaatgcc cgtagagcct ataaaataag gctttgcgga catttaagaa
    28801 gatataaaaa gatagtcaaa aagacatata taatttattc taaaaaaaca acaatttttc
    28861 gttctcattt cttcttttat gttacacttg cgctagcatg gttcaacaga tgaaagcaaa
    28921 caataacacg gttttttaga cccttgtaca aagattccta cataacaaaa tgaggtggag
    28981 aaaatgaaag atttaaggta tagaagtgtc tttgatatta ttggacctgt gatgattggt
    29041 ccaagtagct ctcacactgc cggagctgct agaatcggga aaatcatacg tagtatattt
    29101 ggcgaacagc ctgattctgt agatatttat ctatatgaat catttgcaaa aacctatcgg
    29161 gggcacggaa cagatattgc attagttggc ggcctgttag ggatggaacc cgatgatgaa
    29221 cgtttagctg attctttgaa attggcatat gaagccggta tggaagtctt gtttgttcct
    29281 aagagtgaaa aagccgaaca tccaaactct gtaaagatgc ttctaaaaaa aggaacacga
    29341 aagctttcag tcactggcat ttcgatcggc ggtggcaata tccagatttc tgaattgaat
    29401 ggattcaaaa tttctttaag catgggtact cctaccttga tcgttgtcca ccaagatgtt
    29461 ccaggtatga ttgctaaagt gactagtata ctttctgaga ccaatacgaa tatcggcact
    29521 atgacagtga cacgggaatc aaaaggtgaa aatgccatta tgataattga agtagatgat
    29581 cctcaagtag aagaaacggt aaaaagactc aaacagctgc caaatatcga cagcgtaaat
    29641 tatttcgatt aaggagctaa aaaaatgttt ttatcgattg aagaattagt agatcaagca
    29701 aaagcttatc caagtgtagc agaattgatg attgcagttg aaatggaaat gagtggccgt
    29761 tcccgtagtc acatcatcga gatcatggaa aaaaacctct cagtaatgga acaatcaatc
    29821 aacgagggaa aagaaggtgt tacctccgtc acaggaatta ccggtggtga tgcaaaaaaa
    29881 ttagatgatt atttaaatga ggcagacttt ttaagtggag aaacaatctt aaaagctgta
    29941 cgcaatgctg tagctgttaa tgaagtcaat gcaaaaatgg ggttgatttg tgctacaccg
    30001 actgccggaa gcgcgggtgt tgttcctggt gtgctgatgg ctgtccgaga tcggcttgat
    30061 ttgacacatg aacagcaatt ggaattctta tttaccgctg gcgctttcgg gttagtcatc
    30121 gcaaacaacg cttctatcag tggtgcagaa gggggatgtc aagcagaagt tggttctgca
    30181 agtgcaatgg cagcagcagc tttggtttgt gcaaaaaatg ggactcctca tcaagcagca
    30241 caagcagtag ccattacatt gaaaaatatg atgggactga tttgcgatcc cgttgcaggc
    30301 ttagttgaag tcccgtgtgt caaacgtaat gctttaggct cttctcaagc ttttatctct
    30361 gctgatatgg cattagcggg tatcgaaagt gtcattccgc cggatgaagt cgttgctgca
    30421 atgtaccaag tcggacgtca aatgccgtcg attttcaaag aaacagcaga aggtggctta
    30481 gctgttacgc caactgctaa acgacttgcg gaagagattt taaataagag ggcgtgataa
    30541 agctccttaa tttcgagtat taagagatta gttatcttca taacaaaaaa cgaagagagg
    30601 actaattgcc cttctcttcg ttttttatta atccatcaat ttcgtatttt ttacttcatc
    30661 gatcgttttt gttcctgcaa gttgcatcgt gatcgacaat tctttgttca ggtgttcaaa
    30721 aacagatgtt actccttctg ccccacctag attcaaccca taaatcactg gtcggccaat
    30781 cgcaactaag tcagcaccac ttgccagagc tttgaagaca tgctctcctc gacggacacc
    30841 agagtcgaaa atcactggta cacgtttgtt gactacttcg gcaatttttg gcaatacttc
    30901 aaaggacgca ggacctccat ctagttgacg tccaccatga ttggataccc agataccatc
    30961 tgcccctgca gagatagcga ttactgcatc ttctggcgat tgtattcctt ttacgataac
    31021 tggaagatgg gtgatttctt tgattgtttt gatgtcatct ggtgtcaagc cttgttttgc
    31081 agctgcataa atttctgcga ttccttttcc ttcgccatta ccacttgcac tttgttcgct
    31141 atacgcagct aagttaggca ttggtaatgg aaattggaat tgattgatga cgtcttcttc
    31201 acggtaacca ccgagcgttg aatctgccgt taaaatgata gctttagccc ctgcttctac
    31261 tgctttgttc aaaatgaact cattgaatct gtcgtcttta ctcatatata attggaaaaa
    31321 ctgcggtgcg cctggcgctg catcggctgc gtctttgatt gtcgtatttg cataagtgct
    31381 gatggaaaag attgatccag cagcagctac tcctttagct gtatcagctt cccctttttc
    31441 atgtgccaat ccttgggccg ctgatggcgc ttggataatc ggtgtatcta attcaattcc
    31501 gaaaacagat gtatgcaaat cggctgagtc gattccgcgt aatatccgtg gcatgatttt
    31561 ctttgtatta aaagaagtcg tattttcttt catcgtccac tcatcttcag aaccaccgcg
    31621 aatatagcca aaagctcctg cttccatgcg ctcttttaca cgaccttcta gttaggcaat
    31681 gtttacaata tcgattggat gttcttcatt acttgtatga taagtcatag tagatccttc
    31741 tttcatcttc ttgattacat ataaatcata ctaaacttac aaaaagtaag caaacattta
    31801 ctctcagata cttttatcgc tattctttcc aaataaatcg acttacaagc tgatctactt
    31861 cttggttttc atgaagcata ctatgagagg cattttcacc tgtcactact ttttcctcta
    31921 tttcatttcc gcgttgcttc aacaacgcat agaccgctaa tgcactgttt aacggtactg
    31981 ttccatcact caagtctgtt tcatctagtt gaccagcaat caagaaaaat cgagtggtta
    32041 taggaatatt accgatcatt tgctggtaat cttgatagcg gttagaaacg acagcaggcc
    32101 cctttgctaa ctcatctgtc agagactgcg cactatcatc gacaaaatca ttaaaaggcg
    32161 cgccaattgc tgttaatgtc tgaacttgtg gcaatgacgt atcctgtcca tacgttccta
    32221 aatagcgtag tgcactaact cctcccatgg aatgccccac tatgttgact ttatctatgc
    32281 cgtagttttc tttcagatac aaaagacaag ttttgatcca ttctgcttga ttccactcgt
    32341 tatttttatt atcttcaaac aatacttgga ccatcggatt gtctgctttt ccagacaaat
    32401 ctcctgttgc ttgtatttgt ccatctgttc ctactgttaa aaccaattct tttactgctt
    32461 gctgattttt ttccaaacgc cgcaacatcc ctttaaagga attttctgtc ccgctgtagc
    32521 cgtggaaaaa aagtgttgga atagtggttt tttctatcac tttactggat tctgccgttt
    32581 tttctttatc agaaacttca gtagtattta tcttcgtcgt ctggcaacca cttaacgcaa
    32641 acaaactgat aaggactaaa gctgctgcag ttgcacgttt gcataaattt ttgattggtt
    32701 ctgtcataca cctcttcctt tccatataga aaaaagaaag acttgaaagg ccaatcatat
    32761 gatcgacatt tcaagtctct ttattaatcg attttatcta cttgatcgaa atcaagttct
    32821 gtactggttt cacgtccaaa catatcgatg ttgactttca atttttcttt ttctccgtca
    32881 acttctgtta cttgtccttc taatccagca aatgcgcctt cgatgatttt cacgacttcg
    32941 ccaacttcta cttctagatc attttgtcgc aggctcatac cgatagaacg caagatatga
    33001 ttgacttctt ctggaagtaa tggtgctggt ttgcttccgg ctccgtgtga accaacgaat
    33061 cctgtcacac caggtgtgtt gcggacaaca taccatgaag cgtctgtcat gatcatttca
    33121 acaagtacgt atcctgggaa tgttttatga acgatttctt tttctttccc atttttgact
    33181 tcagtttctt tttcttcagg aacaacgaca cggaaaataa agtcggccat tttcatactt
    33241 tgcgcacgag attcgatatt tgcttttact ttgttttcat atcctgaata tgtatgcaag
    33301 acataccagt ttttttcaaa agattccatt gaaggctcct catttcattg taaaataaaa
    33361 aaacctcagt acacccgaag tttttctttc gcttcattat agcactaaaa gtacgttgtg
    33421 gctagcgagt tattttaaaa tccagccaaa taagctttgg atggctgtat ccattacgta
    33481 aaacatcaat ccgaaaataa tagaagtttc gatgactact aacgtatctt tacggagttg
    33541 ttttctgctt ggccaagtta cttgtttgat ttcatctttt acgcttttta gaaatttcat
    33601 gagacaacct cccttgctac tgtttatttc gtttctttat gtaatgtata ttgattacaa
    33661 tatttacaaa acttattgat ttccaaacgt tcgccgcttt ttccttcact gactgatttt
    33721 gtgtagttgc gagacccaca aacggaacaa gcgagtgctg cttttttctt tgccatttac
    33781 ttctgccccc ttgctgagaa tcagacttat acttcataac agtatcacgt aactttttac
    33841 ttgtcaattt gtgaaataaa ctatctattg ttttggataa aattatgata tgattaattt
    33901 gaaattaagc taaaatgaga caattggagg tatagcgatg ctattaaaaa cacttgtata
    33961 taagaaaaaa gatctgacaa ccgttacaga gactagtaca ttagaagaag cattaaaaat
    34021 tttagaaact tccggatacc gttgtgtgcc gatcttagat tcttcaggta atattttccg
    34081 tggaaacatt tacaaaatgc atatctatcg tcataaagca aatggcggtg atatgacgct
    34141 tcctgtcact catcttttga aaaacgctac aaagttcatt tacctggata cttctttctt
    34201 caaagtattc tttacgatca aagagcttcc ttatattgct gtccttgaca gtgaaaatca
    34261 tttttatggt attttgacac atagtacgtt gcttaatatg ctctctcaat cttggagcgt
    34321 agaacaagga agctatgtac tgacaattgc ttccacagga aagcaaggag atttggctgc
    34381 tattgcaaaa atcatttcaa aacacagcag cattgccagc tgtattactc ttgatatcga
    34441 aaaagacgaa tacattcgac gtacattgat cacgctaccg gctaacactg aagcgaaagt
    34501 atgtgaaacg atcgtcaacc acttagagaa aaagaattat aaagtggtag aaattgaaaa
    34561 tttgcaaaac caagaagcat aatcaaaaag cgaaaaaaga aaacagctaa tctgctttct
    34621 tttttcgctt tttataatta gacacgcttt tcaaaatcaa gcgcgcgatc taaataatac
    34681 atcaatggtg ctgaaacagc catgacgata aactcactta atgcaagcgt gccataggtt
    34741 gtccaaaatg cttggccacc tgttggaact aacatataag caatcagtcc catgctgatg
    34801 gtaaagaaga tcgtgttcaa tactaaacgc atctttacat ttttcacttt gttttgcaat
    34861 aaagctgtca ggcctaaaga aagcaatgtt tggccgccac caaaaaccac atccatgata
    34921 ccaaaaccaa agaaggcatt atagacgatg accccgccta aaactcccca aagcaattta
    34981 cggttaaaga caactaaatg attcaaactt tctgaaatac gaaactggat cggccctgag
    35041 gatacaggcg caactaaaat agttaacgct agataaagag ccatgatgat gccatttaca
    35101 acgattacgg ataaccgttg tctgcttttc gttttttgca ttttctctcc tcttttctcc
    35161 tagttttttt aacgcgggat ggttgatgaa ccgcggttat gtgaaacaaa caataattat
    35221 aacagacttt tcatttattt caatccgtct tatctgcttt taaaaaagca aaagcttaaa
    35281 caatgtaaac gtttgctaaa aatcgtggga tatggtataa agtaagtgta aggggtcata
    35341 agaattccca gtaaagcaga cgaagtagga tctgcaagaa cagaattgtt gtgaaactta
    35401 aataaataaa aaaggatgtg ggaatttgag caacctctta ctagagaacg gtctagcaag
    35461 cagctgaaga tctaagtgga catgccgaac cttggaactt ctccttacgt aagtaatgga
    35521 tcatgcgctt gctaggcttt tttgtgtttt tcggtcttcg gacctttttc attttcctag
    35581 acttctgcta taataaccaa gaattaattt tgtaacagat ttttttgaaa aaacaagttt
    35641 ctttcattca aaaaatagaa aaagcttgct aattattggt taaccagcaa acaggagtat
    35701 taaaatatct tgttttattg aatagcagga ggaaatcatg aaattccatt atcatatatt
    35761 gatcaatcca tcggcaggca gcggaaatgg ctataaagta gcagaaagaa ttttaccggt
    35821 attaaaaaat aagcatattg actatacact tcattatagt gaatacaaag gacacgatgc
    35881 tgagattgct gaaactttgg caaaagaaac attgattcct tggatagaag aagaacagaa
    35941 aacaatcgat actttccccc ttttgatcat cgtaggaggt gacggcacct tacatcaggt
    36001 acttgatact ttctatcaga tggaagtaga attcccagtt gcttatattc cagcaggttc
    36061 tggagatgat tttgctcgag gggcaggcct tccaaaaaat cccaaaaaag gacttcagct
    36121 gattttagca gctcaatcac cagaaaaagt ccacataatg gcttatgaag aaaaaataag
    36181 tgaaaaaaaa ggaatagctg ttaataattt cggtatagga ttagatgcag ctatcgtcca
    36241 tgcgacaaat cattcttcca caaaaaagcg attgaacaaa tacaacttag gatcattttc
    36301 gtatttattc tctattctcc gtgctttatt cactcaaaaa ggatttccaa ttctggtaga
    36361 tgcaggagga aaacaattaa gtttttcaaa cgcattttta tgtacagcaa cgaagcatcc
    36421 tttttttggc ggaggaatcg cgattgttcc aactgctgat gcgtctaaac cagtcattga
    36481 ttttgtcgtg gtcgaacgaa tcaatttctt taaaatcttg tggctcattc ttttacttat
    36541 acagaaaaaa caattgaaat cgaaatattt ccatcactac acaacaaaca gattacgtat
    36601 cgtttcaaca acaccgcaac atggtcaaga agatggagaa gaaatggaaa aacgaccttt
    36661 tgatatcaca ataaccacaa ctaaacagtt gttttggtgt taataaaaga ggttttgtca
    36721 gaagctacat ctggaacacc gtttattcgg aaaaaaggac tgtgacacga cttttgtcac
    36781 agtccttttt ttactatgca gttaattctt tgcccaattg ggttgccatc gatagtttct
    36841 ttctttaccc aaaataaatt ggatcattga aaacaccgca gcaaaagcaa ctaagacaca
    36901 accattgaac caaaaagatt ctgcaaatgt ttgattgtaa ctgagtgttt cactgatcaa
    36961 gttattattt tgaacaaacc aatatgttgg tgtgaatgcc gctattttgc taaccatttc
    37021 tggcaagtat tctgctggta caaacacccc accgataaag caactgcctg tgataaaaat
    37081 atctttgatc ccaccgctgg tttctgagtt ttttaccaat gaagtgatac aagccgaaaa
    37141 gctaatgatc ggcaataaaa acaagaaagt atttaaaata tgataccata ccgttatatc
    37201 ccacgaggtg tggatcacta acagaaccat tagaagtaag aagccactga acaacagcag
    37261 cgagtaacta atcaaactaa aggttacttt gcgattcaag tttcgtttgg taatgggcgc
    37321 actctggttt cgtttactga tctctttgcg attaaatgct agattgatga cgctgaaacc
    37381 gctaaaaatg gatagaaaca acccataaga caagaaatta aaggttgtgc ccgtcaacag
    37441 ctttttcatc cgctgactat atcccgcact tagctcaact tctccagtaa ccgagagtgc
    37501 catttgtgtc tgttccatag ctgcatcgat tgttttatct tcttcttgaa acagaatcag
    37561 tgtttgcaaa aaggtgttca cttgctgtgt aacaactgtt ttagaaaagg catctgggcg
    37621 actctgtatc ttgatcgtcg gcatctttcc tacttcgact tgttcggtaa agtctgcagg
    37681 cagatgtaag ataatgttta cacgattaaa ataaagagca tcatcgattt ctttttgtga
    37741 ggttccatcc aatgtaacga ctgtttcctt gtcattcaaa tatttacgaa ggccatcgac
    37801 tacaggcgac tcttcttcac taatcaaggc gattttcgca tcttgcagag aggtattttc
    37861 aacatcaata ttttgcgcaa aaaagaatgt ggcaacaaca gtgataatta cacctatcag
    37921 taaattcgat ttatttgctt taagcaatgt ccaaatcgtt ttataaagcg tcataacgca
    37981 cccctctttc catccaagca tttcctaacg caaaaaggaa aataaatccg atcaaccata
    38041 gaagattggt ataaaaacta gccaaatcag tgtagaaata aagttt
//
LOCUS       DAJSBM010000020        37245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJSBM010000020 DAJSBM010000000
VERSION     DAJSBM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.020"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..839
                     /locus_tag="ONT67_001235"
     CDS             <1..839
                     /locus_tag="ONT67_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2354988.1"
                     /translation="YQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIF
                     LTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKR
                     NI"
     gene            complement(1263..1433)
                     /locus_tag="ONT67_001236"
     CDS             complement(1263..1433)
                     /locus_tag="ONT67_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2354989.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1627..1806)
                     /locus_tag="ONT67_001237"
     CDS             complement(1627..1806)
                     /locus_tag="ONT67_001237"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354990.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2193..3305
                     /locus_tag="ONT67_001238"
     CDS             2193..3305
                     /locus_tag="ONT67_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2354991.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3383..3676)
                     /locus_tag="ONT67_001239"
     CDS             complement(3383..3676)
                     /locus_tag="ONT67_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR2354992.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3844..4158
                     /locus_tag="ONT67_001240"
     CDS             3844..4158
                     /locus_tag="ONT67_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCR2354993.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4179..6158
                     /locus_tag="ONT67_001241"
     CDS             4179..6158
                     /locus_tag="ONT67_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2354994.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6171..6497
                     /locus_tag="ONT67_001242"
     CDS             6171..6497
                     /locus_tag="ONT67_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCR2354995.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6513..7031
                     /locus_tag="ONT67_001243"
     CDS             6513..7031
                     /locus_tag="ONT67_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2354996.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7031..8383
                     /gene="celB"
                     /locus_tag="ONT67_001244"
     CDS             7031..8383
                     /gene="celB"
                     /locus_tag="ONT67_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2354997.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8467..9972)
                     /locus_tag="ONT67_001245"
     CDS             complement(8467..9972)
                     /locus_tag="ONT67_001245"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCR2354998.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10064..10654)
                     /locus_tag="ONT67_001246"
     CDS             complement(10064..10654)
                     /locus_tag="ONT67_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2354999.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10833..12479
                     /locus_tag="ONT67_001247"
     CDS             10833..12479
                     /locus_tag="ONT67_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2355000.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12528..13247
                     /locus_tag="ONT67_001248"
     CDS             12528..13247
                     /locus_tag="ONT67_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2355001.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13726..14673
                     /locus_tag="ONT67_001249"
     CDS             13726..14673
                     /locus_tag="ONT67_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355002.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14685..15605
                     /locus_tag="ONT67_001250"
     CDS             14685..15605
                     /locus_tag="ONT67_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355003.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15646..17103
                     /locus_tag="ONT67_001251"
     CDS             15646..17103
                     /locus_tag="ONT67_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2355004.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17448..18062
                     /locus_tag="ONT67_001252"
     CDS             17448..18062
                     /locus_tag="ONT67_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2355005.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18055..19779
                     /locus_tag="ONT67_001253"
     CDS             18055..19779
                     /locus_tag="ONT67_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2355006.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19776..21227
                     /locus_tag="ONT67_001254"
     CDS             19776..21227
                     /locus_tag="ONT67_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355007.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21700..23853)
                     /locus_tag="ONT67_001255"
     CDS             complement(21700..23853)
                     /locus_tag="ONT67_001255"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCR2355008.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23855..24901)
                     /locus_tag="ONT67_001256"
     CDS             complement(23855..24901)
                     /locus_tag="ONT67_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355009.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25051..26340
                     /locus_tag="ONT67_001257"
     CDS             25051..26340
                     /locus_tag="ONT67_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCR2355010.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26353..29055
                     /locus_tag="ONT67_001258"
     CDS             26353..29055
                     /locus_tag="ONT67_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2355011.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29040..29897
                     /locus_tag="ONT67_001259"
     CDS             29040..29897
                     /locus_tag="ONT67_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2355012.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29968..30867)
                     /locus_tag="ONT67_001260"
     CDS             complement(29968..30867)
                     /locus_tag="ONT67_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2355013.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31036..33207
                     /locus_tag="ONT67_001261"
     CDS             31036..33207
                     /locus_tag="ONT67_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2355014.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33275..33616
                     /locus_tag="ONT67_001262"
     CDS             33275..33616
                     /locus_tag="ONT67_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2355015.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33892..35646)
                     /locus_tag="ONT67_001263"
     CDS             complement(33892..35646)
                     /locus_tag="ONT67_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355016.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35657..36493)
                     /locus_tag="ONT67_001264"
     CDS             complement(35657..36493)
                     /locus_tag="ONT67_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR2355017.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36495..37178)
                     /locus_tag="ONT67_001265"
     CDS             complement(36495..37178)
                     /locus_tag="ONT67_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCR2355018.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12016 a         6552 c         7639 g        11038 t
ORIGIN      
        1 catatcagct ggtagaaact caagcgccaa cggggtatga attagatgcg acaccgatcg
       61 agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa aaccgactta
      121 caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggggaagtc ctgcaaggag
      181 ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta accacaggag
      241 cagatggaaa actggcgatt gacggcttag caccaggagt gtatcagctg gtagaaaccc
      301 aagcgccaac ggggtatgaa ttggatgcga caccaatcga gtttgagatc gaaagaagtc
      361 agacagcggt ggtagaattg acgaaagaaa atcgacttac accaggcggt gtcgtgttga
      421 caaagatcga tgaccaaagc ggagaagtcc tgcaaggagc tgtttttgag ttacaagatg
      481 ccaatggaac cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgatag
      541 atggcttagc accaggagca tatcagctgg tagaaactca agcgccaacg gggtatgaat
      601 tggatgcgac accagtaact ttcaagatcg aaaaagaaca agaagtggcg atctttttaa
      661 caaaagagaa tagaaaagtt gctgatagct cagatataag aggaaacaat ccaacaagca
      721 gtggaaataa gcatcttcca aaaactggag aaaccatcat tagccagttt atattgtcaa
      781 tattgggtgt gctacttgtt ttcatcagta taaaatttag aaagaaacga aatatatgag
      841 ctaaaataaa aaattgaata agcatagttt ttaaaatcaa ctagtaccca tcaattgaat
      901 ggaaactacc gattttagaa ggggtataca aacaaatatt agtatcggag tagagaaggt
      961 tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg cgttgagaag tgaaaaataa
     1021 acctttgtag taatactagt taataaaaaa tatacaaaaa cactgcgaaa atcattcttg
     1081 agtttcgcag tgttttttgc ctaatgactc aagtttattg ggtaatactt tgattttctt
     1141 tctacaaata atcagcgatc agttgtaatt ttttctctag ttttaatggc tctttgtcaa
     1201 taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct tcaaccaact cgtgaataaa
     1261 ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc gatcaatcac caatttcgca
     1321 tttggtagaa catgtttggt gagctgaaag taggtggcgt tcatgtctgt aactaagaat
     1381 tctatttctt taggattagt gcagctaagg aaatagtttg ttaatcgagg aattttttgt
     1441 gtaggtaaat tttctcgatt aatttttctg tctcgccatc cgcggaaata aaactcagtt
     1501 tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc aacactcaag acagaattct
     1561 tttggaagac tttggtaaat agcttctcaa ctctttcaat gtgcgaataa cggttgtcaa
     1621 agatacctaa caacttttcg aaataaaaaa taacgacact ttttcagtca gtaaagaagt
     1681 gattttatgt ctgacatgat ttgaaatcga gtgccttgga cggataaaat aactttgagc
     1741 gttccaatgg cttcgacagt ttttacaggt atagcgctgc ttttttaggc gtataaccat
     1801 tggcatatga ttatattatt caaagtggac aatggtttcc ttcttcttgt ttttcaccac
     1861 cattaatttc ctatttccat ctaccacagc agaaccacaa tttctacaga cacatggagc
     1921 agggaagaga actgcagcaa cgatcaatgt ttttttcttc tgtaaggttt cgtaagagac
     1981 ctctgtaatc atcaaatttt tgtctattga tctcagaatt tttttgatag aaccattcat
     2041 aaagcatatc gttctctcag ttgtttattt tgtggtgatt taatcataca agaaaacgat
     2101 atgttttttt ataaaaacat gaataggaaa aatagttgac aagaaaatga caacgccata
     2161 tgatgttcca tagtatttac aacctgtcat agatgactgt aaaaaataaa agtaaaaaga
     2221 aaggacgaca acaggtggat ttttatcagt ctctacaact agatccgttt attttaaaac
     2281 aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat gtatatttgt tcattgtttt
     2341 tacgatcatt attcatcgta ttgtttgcca tttgttttat cattataatt acaacattat
     2401 ttgaatcgaa gcataaacca tacgctgtgg ttttattctg tatgttgatg agtattcgtt
     2461 ttgttgactt tggttataag atttcccatt cgatcatagg tttagcaatc gtcatgttct
     2521 cactattagt agcaccgtat gtccagctga tcaaatggtc agttatgggc atgttgattc
     2581 attttatttt actaagcagc atattaatgg caaccgctag tgatcccaaa atggggaatg
     2641 cttctttgta cggcttttca tatctcttta ttgtttattc gttacctaaa gattcgttga
     2701 acaagaattt ctttacacaa acaagtagct tattattttt gtttttttgt tggttttcag
     2761 ttctccttta cagaaaacat cgagaaaaga acaaagacaa aagcttgttt aaagagattt
     2821 ttttgaaagg catgtattcg caggaaaaaa tttggatgct catctatgca tttggcatta
     2881 gtcttttgat cgttgcagga gagtatgtcc cttttcagcg attgatgtgg gcagggtttg
     2941 cattttcttc tattgtatct agctatggat tgatgtcgat tggtttcaaa gaacgagcag
     3001 tagaccgaat cattggctca ttgattggat gtgttttatt tattggtatc agccaattta
     3061 ttccttttaa ctgggtcggt atattaggtg gtctagcttt aggtgtttgt tctacctata
     3121 gatataaaac agtttttaat tcttttggtg ctttggcaat cactgccagt ttattcgggg
     3181 ttccaggagc agtaacgatc cgcatttttg agaatatcct tggggtatgt ttaggaatca
     3241 tgtatattgg ggtgactgaa atacttatcc gaaaaataag agaaaaacac ggtttgaatc
     3301 attagatgag atcgaataaa aaataaggaa aaagtgaaga tacacatgga tgataaattg
     3361 ctatcattcc atgtgtatct ttttacggta aatcagtgaa acgttcgact ttcattttta
     3421 atttatactt tttacggagt tcgtttattt gagccatcat ttcggatttg tgatgctttt
     3481 cgattgcttc ttcatctttc caacggtcga tcagtaaaag agattctgga tcttctagtg
     3541 gaaagtaata gtcatatttc tcgtttcctt cttctgcacg aatcgctttg acaatgtttc
     3601 tttcgaccat ttcttctgca aattttctgg cttgtccatt tgtacttgta tacaaaatat
     3661 tcactgtgat actcactaaa tcccctcctg aaaaaattct atcactagta tatcacggtg
     3721 ccagaaatta ttattcaaga tatgatacac attttttccg caatgctacg gaaatatata
     3781 ttttgaatag aaaacgctta caagatatgc ttggtttata aaaataaaag gagtgattga
     3841 tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt catcctcgat gatggcctca
     3901 aagacaacgg atttctttaa gcaaaaaaga gaagaggtac tcgtagatgc agtttcagct
     3961 acagaaggcg ataatatgat taagaatagt gattttgatt tatttctgat cagcccgcaa
     4021 acgacgatgt ttttggataa attcaaaaaa ttaggagaat ctgttggaaa gcctgttgtt
     4081 agtatccctt tccaagcgta tgtcccaatc cctacaggca tccagaagtt agcagaggtg
     4141 atcgaagaga acatatgatg gaataggagt ggatgataat gaaacagaag gaaaaattgc
     4201 tgttgagata tttgattgat cacaagaagg agtatgtcac cagtcaacgt ttagcttctg
     4261 aattgttatt atctgatcgg acaatccgta attacttgca gcggatcaaa gagttagtag
     4321 aaaaaaacgg aggtaagatc attgctaaac caggttatgg ctaccaactg catattttac
     4381 aaagactgac atttgacttg tttttgagtc ggcaagaaat agtacctgga tatgctagag
     4441 aaccgcaagg attttatgag tccgaagatc gacaaaagta tatattgaac caattgcttt
     4501 tggaagatgc cattcttttt atggatgatt tggcagaaga gctttatatc agtcgttcca
     4561 gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat tccatattca ctaaaaatcg
     4621 tttctaagca tggtcaaggg acatggatcg aaggggaaga aagaaatcga cggcatttta
     4681 tcatggatac tttttttgga aaccattatg gaaattcatt gaaagaatat ttagggcaaa
     4741 gccgtttttt cccagacatc agttttgaag agctagtcat tatcattctc gatgaaacaa
     4801 gagaagctaa gttaaaagtt tcggatttta tcatacagaa tctgacttta cacttggctt
     4861 tggggatcaa acggctccga gaagggtttg aaatcaagga attaggcatc gaacaagaag
     4921 tttctaagcg agtagagtat caagtggcgc agcggatcgt tcaacgtatt gaggtaatcg
     4981 caggtgtatc gtttcccgaa gaagaaattg cttatttgac attacatctc atggctaagt
     5041 cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa ggaattagcg aaagttctga
     5101 aaagattgtc tcaggaattt catttttcat tagtagagga tctccagctt ttcaatggac
     5161 tactcgatca tttgtcaccg atgatgattc gattgagccg aggtattcgc attgagaacc
     5221 cattgactga ggagataaag aaagaaaatc ccaaagtctt tgacgcagta aaaaggcatt
     5281 tcagcaacat gccggctttg aaaaactata ccatcaatga agatgaatgg gcctatttgg
     5341 ctttgcatct catggcggca ttggaaaaag aaagagctgc gcataaacta catgcattga
     5401 ttatctgtgc aacgggttac ggcagtgccc aactattaaa gaatcgcgta gtcagtgaat
     5461 tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta tgagatcaat gaaacaactt
     5521 taaaaggaat cgacctgatt atttcttcta ttgatctttc gacaatgttt ttcaaaattc
     5581 cagttttaca tgtcagtatt tttttgaatg aagatgatgt tcgtaaaata cgaaaggtag
     5641 ttggagaatc tataccctat cattcctcag atagaccgat accagcattt tctctacagc
     5701 aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt tttcaagact tatacgtacg
     5761 aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa gctgagcata aatgaagaag
     5821 aaggatatat aagagaaatg cgaaagcaga tccagcaacg tgaaaatatg ggacaaatca
     5881 tattcagtga tacagtggca gtcccacatc ccgctgttcc agttggaatc gaaacaaagg
     5941 tagccgttgc attgattcct tctgggatgc agtgggatca atatcaagaa attcattttg
     6001 tttttttagt ttcgccttct tatgtcgaaa atgaaggaat tacggtcgtg actaaagcaa
     6061 tcgttaggtt agttgaccag ctaaaagtac agcaggaaat cttagctgaa cctatttttg
     6121 agaatttcag cagtaaattt atcaaactga tcgaataagg aggaaaacag atggctgaaa
     6181 aaataaacag tgaaacacta caagtgactt cttttgatat cattttccat agcggaaatg
     6241 cacggacact gatacatgaa gcatttcgct cgatgcgtaa agagatgttt gaagaagtag
     6301 aagagaagtt gcgacaagca aatgaagaga ttcttcatgc gcatcagtca caaactcaac
     6361 ttctgaaaga atatgccagc gggacaaaaa tagaaatgga aattatcatg gtccatgcac
     6421 aagatcatct gatgacgacg atgacattat tggaagttgc acaggaaatg aattatttgt
     6481 atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga tacagtaaaa aggaaaatcg
     6541 aattagaaaa aaagcaactt agtttgaaaa acatgtattt caatcgttat ctaatcgtac
     6601 gttacctgac tgcctttttc tttttcatga acatgcaatg gatgattctg cttctatcag
     6661 ctaaaagttt aggaagtagt ttaccgatga ttctgatttt agctattttg cctgcagtag
     6721 gagaacaagt aaagctttat cgaaaacacc aaacgaacgt tccatggaca aagtggtatt
     6781 ttttgttcca gagtgtcttt aatttattag tgattccagt gttgtttacc tcaggattta
     6841 cattcttgta tccttttatg gcaaataaca atggaggaca gttatttgtt ttcattcttg
     6901 ttgtaagcgg tatttttgcc agtgcattta tacagtatcg tttaaagaaa atttcactca
     6961 accaagacca acagtatatc cgtattaaac aatatgaaaa agcgttatat ttaggaaagg
     7021 agaataacta atgtttgatt ttttgcagaa atacctgatg ggacctatgg ggaaaattgc
     7081 gcaacttaaa atcgttcggg ctgtcatggc agcggggatg gcttctattc ctttcacgat
     7141 tgtaggctcg atgtttttgg ttttaaatat tttgccatta ccgttcccat ttttagaagg
     7201 attttttaat gcaacttttt tcaaagttag tgacttgtac atgatcgtca atacaatgac
     7261 gatggggatt ttatctattt actttgccat tgtgtttgct tatgaattaa cgtcaattga
     7321 acgagatgaa caaaaattaa atgttaatcc gctcacgggg gcattactat ctgtttttgc
     7381 attctttatg tgtatccctg aattgatcat gactgatggt aaaatcagct tgattgcttc
     7441 tatcaatgag acagaaactg tagtaaatgg tattaggatg ggctcttttg tcgaacgttt
     7501 aggaacttca ggaatcttta cagctattat tatgtcgatt attgcggtcg aattgtattg
     7561 tatgtgtgtc aagagaaatt gggtcataaa aatgccagat gttgttccgc caggagtttc
     7621 tcgttctttc actgctctga tcccaacatt tgtcattgcg tttgtggtga tgatcatcaa
     7681 tggattatta gttgcactag gaacggatat cttcaaaatg attgctatcc catttgggtt
     7741 tgttacgcag ttgacgaata catggcttgg tgtcatggtg atttatttct tgatccatgc
     7801 attgtggatc gtagggatcc atggagcgaa tatcatcact tctttcttga ccccgatcgt
     7861 attagctaat atggcagcta acgcacaagg cgcaaattat ccgttggcag gagaattcaa
     7921 caattcttat gtaaccgttg gtggatcagg tgcgacttta ggattgatca tctttattgc
     7981 attcctcgcg aaatctgatc agctgaaagt gttaggaaaa gcctcacttg taccaggatt
     8041 attcaatatc aatgaaccaa tcattttcgg gatgccaatc gtctacaatc cctatttagc
     8101 ggttccgttt ttcttagctc cgatggcttc tgctacactc gcttattttg ccattcggct
     8161 ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct tcaccagtcg ggatcggagc
     8221 atttgttggt agtggtggtg attggaaagc tgctgtgtta gcagtcattt gtgcaatcat
     8281 cgcattcatt atctggttcc catttatcaa attctatgat aataaattat taaatgaaga
     8341 aagagaaaag gcagcagaat tagctagtga aaagttaaaa tgatttctaa gaaaaaagga
     8401 actgtgacaa aaaatcacag tccctttttt tcttagaaat gggaaaccac tagtatataa
     8461 ataccattat tcttcaatca accgcttcac gattgcataa tccccttcat aaggttcatc
     8521 tacaccattg attttttgat agggatccac gccttttccc gcaataacga ctgaatcttg
     8581 cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga cgatccattt cataatgaac
     8641 gatcaaatct ggattagtaa ttgcttcatt gatttcttcc gcgatttttt tcggatcttc
     8701 aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct gtttctgata gcacttttcc
     8761 aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg cctaatacaa caattacacg
     8821 gccatctgga tgttcatttt tggcaaattt cagcaatgtt ttcaagctta gataattatg
     8881 tgcatagtcc acatagacat gcgctccatt ggattggatc aattgatcca ttcggcctgg
     8941 tacacgagct tcttttaatc cgtcttgtgc atcttgactg gaggcacctg ttagtgctgc
     9001 ggcaatgatc gcactggctg cattttcttg gttgaatcct ccagccagca aaatatcata
     9061 agcagtatcg cagatagcta agcgatcttc atgagaagat aaagtaaaac cgtgagtacc
     9121 tttatctgaa cggatcactt gataattgct ttcttccgaa cgaccataag tgatcgttgg
     9181 aatctggaat agatcggctg tttccctaag taaatcaaaa tagtcacttt catgatttaa
     9241 aacaactgtt tttgaattca agatcaattg tcgcttgcaa tagaaatagt catcaaatgt
     9301 cggatgttcg atcggactga tatggtcagg cgagatattc aagaagattc cgacatctaa
     9361 agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa gaaacttcca tcactagatg
     9421 agtcatacca ttttcaactg cttctgacat catacgatat aaatctaatg actcaggcgt
     9481 tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt gtattcatag tagaaagcaa
     9541 tgctgttttt ttatttgttg tgtgatccaa gattgctttt gtaaagtacg cagcagttgt
     9601 tttacctttt gttccagtga atccgatcac tttcaattta ttttgcggat agtcgtaaaa
     9661 agccatagca agaagcgcca ttgctttacg gatatccgtt acgatgattc ctactgctga
     9721 attttcgtat ggttgttctg atacataata ttgaataccc tgttcgattg ctgaatccaa
     9781 atattcttct ttgaaattca ttcctttaca gaaaaacaaa gtagaagcat cagcttttct
     9841 tgaatcataa cttaactgaa gaaattcagc tttttctggt acatcaagat gccagcctct
     9901 tgttgaaaca aattctttta atagattttc ttttaatagt aaatgacgga tgtcttctaa
     9961 agagaagctc attgattgat ccctcgcttt attcaatttc ttggtttatt atagcacact
    10021 ctttttaata tatcgtttgt ttccgctatg acaaaatcca gctttattca aattttctcg
    10081 acacagggga caagcggatg agtattttca taaacaatga actgactgta atagatagga
    10141 gaacgaatag tgtctccaac aatccaaaaa cataataatt caaaaagacg ataagactat
    10201 tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa tttgtggaaa ataatcggcg
    10261 gtatcgttat accgccattt gaggccccga ctgtgtacaa tgtgtaaacc ccaagactga
    10321 acaaaagact tcctgaaatc agtgaaaaga aatgtgagga gatcatttcc acttttggaa
    10381 caatctctaa aacaacagga aaaagcagac ttcccgctac agtcttaaaa aatactgatt
    10441 ttcctaatag gacagcggct agaagcagca tcataagatt gatgctccaa acagtaactg
    10501 agcgttggat agaaaaggcc gtttcaacta aatgtccgat ggcgcccact ccggcggaag
    10561 ctatatcgtg agggagcaag aaccaattca atccaactgt gataaagaaa agacctaaaa
    10621 atataacggg tagttttttt atttcttgtg tcattgatat ctcctataaa tgttagccat
    10681 aaaaggctgt atatttcttt actttaccaa aaaaacgaaa ccgaattcaa aaaaacaatt
    10741 gttcttataa aagttatata aaatcgattg ccagtttctg ttttaccaat taaaatgata
    10801 gtaatgtttt gtagaaagtt ggaatctgac cgatggataa tcaaagaaaa ccagcattag
    10861 atcaattaac accgcaagaa aaaatggcac gaggctctgc ttggatgacc gccagtaata
    10921 tcatttcaag attgctaggc gctgtctata tcattccctg gtatgcttgg atgggcgaaa
    10981 atgctaaggc tgccaatgga ttattcaata tgggatacaa tatatatgca ttgtttctac
    11041 tgatctctac agcaggaata cctgctgcga ttgcaaaaca aactgcacgc tataattcat
    11101 tgaatgaata tggcacttca cgacgattat ttatccgtgc attacagatg atgggcgggt
    11161 taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc ttggctagct catgcttctg
    11221 gtggcggaga agaactgatt ccaacgatgc gttctttgag tatcgctgta ttggtttttc
    11281 cttgtatgag tgtgatacga gggtatttgc aaggcaatca agaaatgatg ccttatgcgc
    11341 tgtcgcagat tgtagaacag gtggcacgta ttttttatat gctattggct acattcatca
    11401 tcatgaaagt catggatggt gactatgtga cggcggtcac gcaatcaact ttcgcggcat
    11461 ttatcggtat gctagcaagt attcttgtcc ttttgtattt tttgaagaaa catcaagcgt
    11521 atacatcggc gtttattcac tacagcgaaa acaaaataaa tattgcaacg aaggaattgt
    11581 tgttagatac cgttaaagaa gcgattccgt ttattattgt gggttcaggg gtaacgatct
    11641 tcaaattagt cgatcagttc acttttatga agatcatgtc tgcatccacc gagtattcca
    11701 atgcacagtt actggatctt ttttcgattt ttagtgccaa tccagataag ttgacgatgg
    11761 tagttattgc attggcaaca tcgatcgcgt ctactgggtt accgttgatt acagaagctg
    11821 ttacgttgaa agatcgagta ggtctggcaa aattgacaag cggaaattta cagttgtttt
    11881 cattttttat gtttccggcg acatttggtg ttatgttact tgcctatccg ctaaatacat
    11941 tattttatac accggattct ctaggaagta atgtactgat ccaagcaagt tttgtaggac
    12001 tcttcctagg actatatatg ctagtttcca acatgctgca aggaatgttt gaaaacaagg
    12061 cggcaattaa ttatttggtt attgggtttc tagtgaaact ccttcttcaa tacccagcca
    12121 ttcgtttatt tgaagtatac ggccctttat tggcgacaat gatcggattt gctgtgtctt
    12181 gtaccttgat cttaaagaga atccatgaag tagcccgctt taaaccaagt tttgtctggc
    12241 gcaggacatt gctgatcttt attcttacct tgattatgct tttggcttcg tggatcacaa
    12301 agatgatttt tggtgctttt ttaaacgaag atagcaaatt ccagtctcta ttgttgattc
    12361 ttgtagtagc aggtataggg gctttagtgt atggttattt agctttgaaa atccgcttgg
    12421 cagaaagact actaggacca ggaatagcgc gtttacgaca taaattaaaa ataaaataag
    12481 tgaatcagaa gagctagatt gatgctcttt tgaaatgggg gaaacgcatg cgtttagata
    12541 agtttttagc agatatggga cttggcagcc gcaaagaagt caaaggatta atcaaaaaag
    12601 gaacgatcga agtcaatgga caagtggtga aaagcgataa agcgcaagta aatgaattcg
    12661 aagatgcggt tacttattta ggagaaatca ttacgtatca aaaagatttt tactacattt
    12721 tgcataaacc tgcaggagtc atttctgcta ctcaggataa tcatgatcaa accgtaatcg
    12781 atttattgga agaccaggat tatcgggaag atctttttcc agtcggccga ttagataaag
    12841 acacagaggg cctcttgatt ttaacgaatg acggggtgtt tgcccatcag ttactatcac
    12901 ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag cggtataatg acagaagaag
    12961 atatccgttt atttgcagaa ggactgatta ttgatggcga tgagcagaca ttgccggcaa
    13021 aactccaaat tgatcaagta gatcaagaga cagaaacttc aattatccgt ctgattttgc
    13081 atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc agtaggaaaa gaagtcttat
    13141 atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa agatttgaat aaagggcaat
    13201 atcgacccat gaccaaagaa gagctagaac aagtgagaga aaaataattg aatggatagc
    13261 taaaagaata agcagagtag ttgtagcgcc atctgaaaag cagacttgtc cgtctgactt
    13321 tcggatggct tttttgtgaa atttcggctt atttccgtag aagctacttc tggaacaccg
    13381 tttgttcgga ataagggact gtgacaagac ttttgttaca gtccccattg ttccttgtga
    13441 ctcagagtta gatagtaagg acacgatctc cctaatactc aggccaaaac gctgaattca
    13501 caacctctga taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    13561 aatatgagga actattttcc cgtttttttc tctaatactc aggccaaaac gctgatttca
    13621 caacctctcc tatactttgt ttcattttcc ctatactttg tttatttatt ctcattattg
    13681 aaagcgctat aattacgttg cgaatgaatc gaaaggggtt tttagatgaa aaaacgaaag
    13741 gggttcaatc atttttttat gaatctatgg cgttataaag cacttgtcct gatggctgta
    13801 ccgggtatgc tttggatgat ttttttcttt tacatacctg taatggcgaa tgtcgtagca
    13861 ttcaaaaatt ttcatatttc agcagatggg ttcttggcta gcttgaggga aagtccttgg
    13921 gtgggttggg aaaatttcaa atttttattt tcttcggatc aagcatttct catcactaaa
    13981 aatacaattt tatataatgt agcatttatc cttttgaatc tgctgatctc agtcgtattt
    14041 gcaatcatta tgagcgagtt gagaaataaa cgactcgtta aagtttatca aacgatgtcc
    14101 ttactacctt attttctatc ttgggtcatt atcagttact ttgtttacgc ttttttaagt
    14161 cctgacaaag gaatattcaa tcagtggatc actggacatg gaggagaagc tgtcaattgg
    14221 tataatgaac cgaaatattg gccgtttatc ttggtgttca ttggtacatg gaaagggatt
    14281 ggttataaca gcattattta ctttgcatct gttatgggaa tcgatccgac ttattatgaa
    14341 gctgctatgg ttgatggagc tagcaaatgg cagcagatca aacatgtgac gataccacaa
    14401 cttattccac taatgacgat tttaacgatt ttagccgtag gaaacatttt ccgagctgat
    14461 tttggcctgt tttataacat tcctaggaat tccggtgcat tatatgatgt gactcaagtt
    14521 ttggatacgt atatctacaa tggcctgaca tccacaggag attttggtat gacggctgct
    14581 gcaggacttt accaatcagt tgtcggcttt gtcttgttga tgatcacaaa tacgatcgcc
    14641 cgacgttttg atagcgattc tgcattattc taaggaggaa aaaaatgaaa aaaaagaaag
    14701 tggagagagt ccaagtccgc tctttcaata agacggctaa ttttttctct aatataatga
    14761 ttgcactctt tgctatttcc tgtattttgc catttatatt tgttatcgcc atctctttta
    14821 ccgatgagtc catattgtcg actcacgggt acagtttttg gccagaaaag ttcagtatat
    14881 ttggttatac gtacttgttc gaccagatgc aggataaaat tttccaagcg cttttcgtca
    14941 ctgtacttgt tactgtttta ggtacactga tcaatagcac ggctacttct ttatatgctt
    15001 atgcgatttc acggtcaaac tttccattcc gtcgtttttt taccgttttt tgtttgatta
    15061 cgatgttgtt ctcaccaggg atggtggcca attatcttgt tatgacaaat cttcttcagt
    15121 taaaagatac gatttgggca ttgatcttgc caatggctgt cagtcccttc aatattattg
    15181 tgatgcgcac attctttaaa cgttcggtct ctgattcgat tattgaatcg gcacggatcg
    15241 atggagctag cgaactgcga atatttgttc agatcgtttt accgttagct gttccaggta
    15301 ttgcgacaat cagtttattt gccgcattgg gttactggaa tgactggttc aatgctttac
    15361 tatacatcca agatgataag ctagttccac tacagtatct tttgatgaaa atccaaagta
    15421 atattcaata tctgacacaa aattcaggag caggaagcca attgacaggc ggattagcct
    15481 cagttccagg agaatctgcc agaatggcga tcgtagtcat ctcgacgttg cctattgcga
    15541 tcagctatcc atttttccag aagcattttg tcaaaggtct aacaatcggc ggagtaaaag
    15601 aataaagaaa caaaaatata aatcataaaa ttaggaggaa gaaaaatgaa gttatggaag
    15661 aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa cattagcagc atgcggaggt
    15721 tctggttcta aagaccaggc agaagctagc aattcggaca ctttgcagat gtatcaaatc
    15781 ggagataaac cagataattt cgatcaattg atggaagtag caaacaaacg aatcgaaaaa
    15841 gagatcggtg taaaagtcaa tatcaattac attggctggg gcgattacga aaagaaaatg
    15901 aacgtcatca tttcttccgg ggaaaattat gatatcgcct ttgcgaacaa ctatgtttcc
    15961 aatgcccaaa aaggagcctt tgctgatttg actgaattag cgcctaagta cgctgaaaaa
    16021 gcttataatg atttagatga agcatatatt aaaggaaatc tggtcaatgg aaaattatat
    16081 gctttcccag tcaatggaaa tgtctttgct caacaagtac ttacttttaa caaagcttta
    16141 ctagataaat acgatctttc aattgatgga atccaatctt acgctgatgc agaaaaagtc
    16201 ttgaaggaat tccatgaaaa agaaccaaac acggcagctt ttgccattgg acaaggcttc
    16261 aaagtccaag ggaactttga ttatccttta gggaatacgt taccatttgc tattgatctg
    16321 gatggagatg attcgaaaat catcaaccaa tacgataatg aaacgtatat cggactgtta
    16381 cgtacaatgc atagctggta caaacaagga ctcattccaa gtgacgctgc aacgagcaat
    16441 aaggattatc cactagaagg aaatacttgg ctaatgcgtg gagaaacaca aggaccgtat
    16501 gattatggcg atacaatttt gacaaatgca gcaaaacaag aacttgtctc aaaagcaatt
    16561 actgttccac taaaatctac agctcaagca caaatggcaa attttgtcgt atcaaatact
    16621 tcaaaaaata aagaaaaatc agtagaactt ctagggctgc ttaacagtga tccagaatta
    16681 ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg aaaaagtcga tggttcagat
    16741 gataaaatca aattgctaga tggttatcag ccaaatacac atatgtctgc ttggaataca
    16801 ggaaacaaca agattctata tacacaagag tctattacgg atgacatgat tgccaaacgt
    16861 gatcaatcga ttgctgatgc agaaacttca ccgattttag gattcagctt caacactgac
    16921 agtgtcaaaa cagaattaag taatatttct aatgtcatga atcaatattt agatggattg
    16981 aatacaggga ctgttgatcc agatgaaaca ttaccgaaat tgaaagacgc tttggacaag
    17041 gctggctatg ataaggtatt gaaagaaatg caaaaacaat atgatgagtt ccgacaagaa
    17101 taataatgaa taaagacaaa ggggttgtga caaaagacgt gtcacaacct cttgttccga
    17161 ataaacggtg ttcaaaagcc agtttctcgg tattaagcta aatatatgta aaaatgtgga
    17221 aagccatatt cacaaaattt ttcttactac tcgaaactaa gcggctattt cacaaactct
    17281 tggtaaacgg tgttcaatca gctaaccctc ggtattcgct cacaaaacga caaaatatga
    17341 ggaactattt ttccgtttgt ttctctaata atcaggtcaa ggtgctgatt gcccaacctt
    17401 tttttgtata atgagaaagt ataaacttaa tgtttggagg agaagatatg ttagggaagg
    17461 ttttggaaga gttatttatc cggatctggg tcattataaa actaaccttg tatttctgga
    17521 tatatacatt tgccggcggg atcatctttg gattaggtgc ggcatggaaa acagtcaatg
    17581 aactatttta tttgtacgga ttcgagtata aagaaatcac aataaaaaga ggctggaata
    17641 tatataaaag aaatttttta agaggcaatc tgctatttag tttattttta tctggtactg
    17701 ctttattgag ttacaattta tatttatctg ttcaaatcaa agggctcatt tttttagcta
    17761 ttgattttat tttacttttt gccttgctct gcctatttgc tacttatcaa tacagtatga
    17821 ttttagatag tgaatatacg atctctgttc ctaatctgct aaagctaagt tttatttcgg
    17881 ttttttccag cttttcgtct tttctaaaaa taattattgg tagcggtata attttaggcg
    17941 tgacttggca attcaaagga ttgatcctgt ttggtgtcat tggtcttttg acggtttgga
    18001 atggtacgat gacaacacat tggcgtgaag aattagataa acagttggaa agttatgaat
    18061 aagtttcgga ttctattttc aaaaggaacc ttactaaatc gtctgttgcg gaaatacgcg
    18121 gttatcatga tcggtgtaac gatgactgct accgttattt ttagtatcca tacttgggaa
    18181 caaaatcaaa aacaggcaga aaacatgaca agtgatgccg ttcaaagcac aagcagaatg
    18241 ctgaatgata aaacaacctt gagtaggatc atcaaaaacc agcttgtagg gaattccgaa
    18301 aagatcgaaa atgtgacgac ctacttaaca aaacccatag atcagtatct gatgtatgta
    18361 tacgaacagc aaaacagtac cgatgaatta gtttctttcc caaatcaaat caaagatctc
    18421 tacgcaaatt atgaagaact aagtgcgata tatatcgtgt tgaatcaact accggagtat
    18481 tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa aggggacacc aaaattacta
    18541 gacgcctttt atatcaagtt gcctatcaca caaggaggag ctgagtcagg aagtattttt
    18601 attggttttc aaaagaaaga actagatatg attttagaaa atctaacgtc attcaatgga
    18661 ctctctttat acatgatttc tggaacgaca aatcgactat acacattcca tgataataaa
    18721 atagctaaag aaacgtttga aagacaagaa atgatcatca cagagagtct gaagaaaaat
    18781 tcacaattgc cgattgacga tctggaaaaa aacaattttg ttcaacacca agaactgtct
    18841 gatgattatc gtatattagc tgtactagat aaaaaaagtg taaaaatcga aacgggaaaa
    18901 aatcttgccc cgctgatcat tggtatcatt attttagaca atatactgct tatctttctt
    18961 tataaaacat tcaaacgata ttcacagcaa gtatctatcg tcatgcaggc catggatcaa
    19021 acagcagcag ggaatttaga gacgcggatc gacataacaa acacggaata tgaactgagg
    19081 gaactttcaa tcggtatcaa cgaaatgttg gatagcatca atcagtttgt agaagacatc
    19141 tacaaattag aaatcaaaca gcaagacgca catatgcggg cgcttcaagc acagatcaat
    19201 ccacactttt tatataatac attagaatat atccgaatgt atgcaatcag cgaaggaagt
    19261 gaagaattag cagatgttgt gtatgcgttt tccgctttgc ttcgaaacaa tacgaatcaa
    19321 gagaaaacga tcactttaaa agaagaactg gacttttgcg aaaaatatgt ctatttgtat
    19381 caaatgcgtt atcctaatcg agtagcctat cattttatga tcgatcctga tttggaaaag
    19441 atcgaagtac ctaaatttgt gatccagcca ttagttgaaa attattttaa acacggtatt
    19501 gattttactc gattcgataa tgcgctaagt gtcaaagtct tgcaggaagg caaacgggta
    19561 aggatcatca tcaaagataa tggaaaaggg atgactgaaa aacggctaaa acagattgaa
    19621 gaaaaactgt ctcatcctaa agtggaactt cacggttcta tcggtctgca aaacgtgaat
    19681 gaacgtttac gtgccagctt cggcagcagt tattatatgt cgcttgagaa taatgagaca
    19741 ggtggactga ccgtttctat tacattcaag gagggatgag cgtgtatcaa gtgttattgg
    19801 tagatgatga atacatgatt ttgaatgggt taaaaaagat aattgattgg caaagtttag
    19861 gctttcaaat agtagcaaca gcagagaatg ccaaagaagg actagctgta cttgaacagc
    19921 gacagattga tttagtagta acggatgtga caatgccaga aataaatgga ctggaattta
    19981 ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat gattttatca ggataccaag
    20041 aattcgatta tttgaaagga ggtatgcaat taggcgccgt gaattatttg atgaaacccg
    20101 tgaataagtt cgaattggtt gaaagcctca agaaaatcaa aacacggtta gatcagcaaa
    20161 atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt attcagtcaa tggttgaatg
    20221 aagaattgga tgaaataagt gaagaagaaa taataggaca aataagtgag aagcagcata
    20281 ggatagtatt gattcaactg gcaagaactc atgatactct tatcaataga tggttgcaag
    20341 aacggcaaca aatcttttac tatcaacgaa attacggaga ccgtgtattg atcacgttgt
    20401 tgttggaggc agatgaagtt gatggcttct gttgttttgt tcaagaaaac tttcctgatc
    20461 aggaatggct gatcagtatc ggggaagaga cagattcttt ggacaaaatc cctgaaacat
    20521 tccaacaagc gaaagacaat ctacagttgc atcaatttta tggagataaa gacgagcatg
    20581 ttttttacgc agtacaagca gaaacaaaag atcaaaccat cgatttttca agtttcagac
    20641 gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat gatcactgat ttttttgaac
    20701 agtttcagtt agcagccatg atgccggaag atatacgata ttccagtttt ttactgttta
    20761 tggagattca acgggaattg attgatctgg aagatgagga atatttacaa ggaatcgaaa
    20821 aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct acttctgtca ttcatcaaaa
    20881 agcatcagag acagaaaaag tacagtgaca atgtggaaaa agtaatcgta atcctgcacc
    20941 agcattatca agaaccactt acactcaaag aagtgtcaga aaacctgcat ttaaatgtca
    21001 tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag tttttcagct tatttgaacc
    21061 atttacgtat ggaaaaagcg aaacaactgc ttttgcacag taatcaaaac atcaatgaga
    21121 tagcaagtga gattggctac aacaacacca cgtatttctc taaattattc aagaaaatcg
    21181 tcggcagatc ccctaaagag taccgagaaa acagtggaga atcataaaag aaaaagccaa
    21241 tgtaatgaaa taacttataa aaaattgtct agaaatctaa ttttgggctg ttcttattac
    21301 ttgaggcaag gcagcttttt catgacctct cgataaacgg agttcaaaag ccagtttctc
    21361 ggtattaagc caaatttatg caaaaatgtg gaaagccatt ttcacaaaat ttttcttaat
    21421 actcgaaact aaacagcttt ttcacgacct ctccactaaa cggtgttcaa tcagctgacc
    21481 ctcggtatta gctcaaaaaa tggcaaaata tgaggaacta ttttgccatt tgtctctcta
    21541 atactcaggt cagaacgctg atttcacaac ctctcaataa acggtgttca aaagcttgca
    21601 tctgcggtaa taagaccaac caaacgaaaa atatgaggat ctatttttgc ttggctgttc
    21661 ttattacttg atgcaggaca gctttttcac aacctcttgt taattatagt ttgataagga
    21721 aaaaggcaaa tcttttgctt gatatttctt agggtgaggg atgatcccta attggtaaga
    21781 gatcgtgccg cctttcagca aatcttgatg ggtaaaatat aatttcgtat attctctgtc
    21841 catccgtttg attttatgga taaattgttg ctgcggccga ttaggcgttg cttcaatcgt
    21901 caattccttt ccatttgaca aaagcaaaac agccttgtca attaatggca taccgataac
    21961 atattcaccg gttccaggcg tgacaggata aaatcccaga ctattgaaga tataccaagc
    22021 ggccatactt ccattgtcct catctccagg gtaacccatt ggagaatcgt cgaaggtctg
    22081 agtcatcaac tgtttaagca gtggctgtgc catttcaggt tgtcctatat aactgaacaa
    22141 gtaaggatag tgaaaacttg gctggtttga aatagctaat tgtccaaatt ctatcgcggc
    22201 catctcactc atttcgtgga tctcaaaacc atatcctcct gggtcaaacg ttgggggctg
    22261 attgcacagc tcaatgagtt tttctttata tctctcactg ccaccatatt ttccgatcag
    22321 tcctgaaaaa tcgtgataaa cagcaaagct gctttgccaa gcacttcctt ctgcataatc
    22381 ttgtccccaa cgattagctg aaaatggttc tctgaacttg ccatctttat cttttgaacg
    22441 cataaatcct gactcatagt caaagatatt ttgatagttc aatgattgct gctgatattt
    22501 cttcgtcatt tcctcttctt ctagtacctt ggcaacttgg ctaatacaaa aatcgctata
    22561 agcatagtct aatgtgtgat tgactgattc gtggtagtga ttaggaacat agccatatgt
    22621 caagtaatct tctgttcctt ggcgtccata atttggatgt ttgctttgaa ccgtagcacc
    22681 tttttccatt gccgttagaa attcaggcat caagtctcgt cggatatctt ttacagcagc
    22741 atctgcaatg accgcatcga tcaatgtccc aggcataagt ccacgttcat caggagacag
    22801 ccattttggc aaatagccac tttctctata gctgttcaaa aaaccttcca gcatttcttc
    22861 gtatttttct acagcaatca aagaataaag aggataaacc gttttatacg tatcccaaaa
    22921 cccattattt gtatagagaa caccaggttt tatagatcga ctagtcgtat catagtggat
    22981 tttttgccca ctaggatcaa tttcataaaa tgtttgtgga aataaaaaca cccgatataa
    23041 attatgataa aacgtccgca attgttgttg atcatggtgt gtgagctgta tacgatcaaa
    23101 ataggactgc cattgttctt gactgtgagc aaggtaagtc tctttgcttt ggttttgttc
    23161 tagttccagg ttataagctg cttgttccga gctgataaaa gatgtcccaa atcggatagt
    23221 ttgttttttt gtctgttcaa aatacaacgg gatcgagtcg ctatttttgt tggaaacagc
    23281 taactcatca attgttaaag gatattcaaa tctcaatata aaataaaaag aaaaatcagg
    23341 atcttctgaa ccagcaaaat gtgtgatttt tccttccact gtgtaaggat ctataacaaa
    23401 gagctgatgg cgtcccggaa aggataaaag taaaccgctg tcatttttgc tgtaatcaat
    23461 agataagatt ccgccataca tgctaggtat caatgtagaa cagatgccat aacgtaattg
    23521 acagatcgac agatgagtcg ggcaaaatat actttcttct gggcggtaag aactttgagc
    23581 atggaaaagg gtgttctcct gtagttttcc attgatcggc gtaaacagca tatgactgaa
    23641 atcacccatc caaggacttg gttgatgggt aagacggtag ccttgaaaaa cgcggtcatc
    23701 aggatgaaac caccagctgc cgttttgatc cgtagtttgc ggtgcaaaat aattcatccc
    23761 aaaaggaacg ccagtataag gctggcagtt cccattagag aaatttggct gattcgctgt
    23821 tgcatgtcta gtatctattt gaccgatatt cattttattt ctcctttgtt gattgtcttt
    23881 tgatgaatga aactggaaat ttactatcgg atggtcgatc ttttgtctct aaccaagtga
    23941 ccaaagcatc tcccgcaagc ttacctaact gttcaaaatc ttgtgcgatg gttgtcagtg
    24001 gcgggtcaac aagagctgct gcttggatat catcaaatcc gataattgaa aaatcttcag
    24061 ggatatgata accatgctgt ctcaagagat tcatggtctg gatagcgaca acatcgtttt
    24121 cacaaacaaa agcagttact tcatacttag tgatataaga aagcaattca tctaatgtcg
    24181 cgcttttgtc atccagtgaa gtgtggaaag tcaaaccagc tttgtccaat gcttcaaggt
    24241 aaccaaggta acgctgtcga acggattgtg gatgagtctg cgtaccaaac aaataagcga
    24301 tttttgaatg tccctgttcg atcaaataag aggcagcaag cgcaccgcct tggaaattat
    24361 cggaaagaac ggtaggaaaa ggtaactcat agattttttt atctaatatg acgacaggga
    24421 attctttcaa tgaaagctca aaaagtaagt caagatgttg ttctgtatca agagcataat
    24481 aaatcaatcc gtcaaattca ttgattagct cttttgctgt tttattttgt agaaagtcaa
    24541 gcgtcgtcat catgacatca atctgtttat cttgcatcac agggttcaat cccttgctaa
    24601 aatcaccaac agacaaatca gcgacatatg gaagaagaaa cagaatccgt cgtgtttttg
    24661 taggagaagc aagaatctgt tccggagaag cggatttaac gaaactgcct ttcccgcgaa
    24721 cgcgataaat aagtcctaat tgctctaatt cagtcaatgc acgtttggaa gtgattctgc
    24781 ttactttata ggtacgtgaa agttcttttt cagttggtag ttggctgtct acaggtaatt
    24841 caccagttaa aatagccgtt tgcaaatcat taaatattgt ctggtacaat ggttgagtca
    24901 tataaacacc tcttaatgat atatcatatt caaataatac cataatggat gaaaaagtaa
    24961 ataaaaagat attttttgaa agcgtagaca taattggctg tatgaagtat aatctttaaa
    25021 gatatatcaa atagaaagaa ggttttatta gtggtttata aagatatacc aaaatcggtg
    25081 cagaaattta tggaagatat cacacaaaaa tgtggggata tccatgcgga ctgggcaaag
    25141 aattttacag cagcatttgc caatactctg ttgacaacag tcaaaaagca aaatgacgga
    25201 accacctttt tattgacggg agacattcca gcgatgtggt taagagactc tactgcacaa
    25261 gttcgtcctt atttagtgat tgcaaaagaa gatgaggaat tagctgacat gatcagcggg
    25321 ttagtcaaaa agcaattttt ttatatcaat atcgatccat atgcgaatgc cttcaacgaa
    25381 gaagctaacg gtgctggcca ccaaacagat catacaaaaa tgaacgattg gatctgggaa
    25441 agaaaatatg aaattgattc tttatgctac ccaatacagt tggcctatct tctttacaaa
    25501 aatacaggaa aaactgatca attcaatcaa tcttttattg aaggtatcaa aaaaatcttg
    25561 gacgtgttca aaacagagca agatcatgaa cactctcctt atacatttgt acgagacacg
    25621 acaagaaaag aagatacgct gatcaatgac gggcgaggtg caaaaacagc ttttacagga
    25681 atgacatggt ctggtttccg tcctagcgat gatgcttgcc gttatggata tctggtacca
    25741 tccaatatgt ttgcggtggt cgtgttaggt tatgtggaag aaatttttca aacgatactc
    25801 gatgataaag aaattgtaca gcaaacacag aaattgaaga aagagatcca agaggggatt
    25861 cagaatcacg gacgaacggt taatcaaaaa gggcaaacga tttatgcata tgaagtagat
    25921 ggaatgggaa acgccacagt catggatgac agtaatgtgc ccaatctgat cgccgcacct
    25981 tacttgggat attgttctga acaagaggaa gattatctgc aaacaagaag aacattgttg
    26041 agtaaagaaa atccatattt ctatgaagga aagtacgcaa aaggaattgg atcctcccat
    26101 acgccagaaa aatacgtttg gccgattgca ttagctatgg aaggaatgac aacgtcagac
    26161 aaatctgaaa aagagcggat attgaattta cttgtagcaa ctgacgcagg aacacacctt
    26221 atgcacgaag gattcgatgt gaacaatcct gaaaattata cgagagaatg gttttcttgg
    26281 gcaaatatga tgttttgcga attagtcatg gattactttg atattagagt agaaaagtag
    26341 gaggtatcgt aaatgaaaaa gaaagtatat atcatttctc atagccactg ggatcgtgaa
    26401 tggtacatgg cttacgaaca gcatcacatg cgactgattg aattgataga cgatctacta
    26461 gagctatttg aagttgatcc gtccttcaac agttttcatt tggatgggca aacgattatt
    26521 ttagatgact atcttcaagt aaggccagaa aaaagacagg caatccaaca agcaatcaat
    26581 gaaggaaaat tgagaattgg accattttat atcttgcaag atgattttct tatcagctcg
    26641 gaatcgaacg tgagaaatat gctgattggc atggaagaaa gccgtaaatg gggaacccct
    26701 gtcatgttag gctattttcc cgatacattt ggaaatatgg gacagacgcc tcaattaatg
    26761 aaacaagctg gtatttcagc agcagcgttt ggccgtggtg taaaaccaat tggctttgat
    26821 aatcaagttt tggaagcaga aaactattct tcccaatact cagagatgtg gtggaaaggc
    26881 cctgatcaaa cagcaatctt tggtctgcta tttgccaatt ggtatagcaa cgggaatgag
    26941 attccagtag aaaaagaagc agctcttgcc ttttggaagc aaaaattagc ggatgcagaa
    27001 caatatgcct ctacgaacca cttattgatg atgaatggtg ttgatcatca accggtacaa
    27061 aaagatatat caaaagcaat ccatctcgca aatgaactgt ttccagatta cgagtttatt
    27121 cattctaatt ttactgacta tctggaagca gttcaaaaag atgtgccaga agacttaggc
    27181 tcagtagaag gagaattgac aagtcaagaa acggatggct ggtatacttt agccaataca
    27241 gcatctgctc gggtttactt aaaacaatgg aataccaagg tccagcgaca gctagaaaat
    27301 attacagaac cacttgcgac gatggcttat gaagttagtg gaaactatcc ccatgatcaa
    27361 ttagattatg cttggaagac attaatgcaa aatcatcctc acgacagtat ttgcggttgt
    27421 tcagtcgatt ctgtccatcg ggaaatgata cctcgttttg aaaaagcaga tgaagttgga
    27481 aagtatcttg cacaagattc cttagaacag ctgaccgcag caatcgatac gaccggattc
    27541 ccaaaagaca gctttccatt tgtcattgta aatactgcag gaatggataa aactggtgaa
    27601 gcagaaataa ccatcgaatt agaaagaaaa agattcgctg aaggaatacc tgaacaacta
    27661 tatcaagagt tagaaaattt gcctaaacgt aagtatcatg tagaaacaaa aagtggggcc
    27721 actataccag ctattttatc tgaagaaact gtccaatttg gttatgattt gccgaaagac
    27781 cgtttccgtg tgccatatat ggcaagaatg atcaaagtga ctttaccatt agaaaacatg
    27841 ccagcatttt cttgggaaac attcgcgctt gtagaagggg aagcgaaagc agaagaaaag
    27901 gaaacaatga ttcatcagag tggacgaatc atcgaaaacg gccctctaca tttgacgatt
    27961 gaaaaaaatg gtaccattac aatggaagat cgcaaaaaca aacgaaagtt gaataatctg
    28021 catatttttg aagatatcgg cgatatagga aatgagtata tctttaaaca acctttctgt
    28081 gatcagccaa tcctatccag taacaaagaa aactctgaag taaaagtatt agtagataca
    28141 ccggaaatag caaaaataag tattctacag gaaatggaaa tccctgtttc tgcagacgag
    28201 cgtttagaaa aagaacagca gatggttgtg gaatttcgtt atcgaaaagc agaacgatcc
    28261 aaagaaaaac gaatccttca aataaaaaca atcatgacaa tcagaaaaga tagtaaaaaa
    28321 atcgactttg aaacgactct cgacaatcag atgaaagatc atcgcttacg tgtcttgttt
    28381 cctacaaagt tacatgtgga acatcacgaa gcagatagta tttttgaagt ggtgaagcgc
    28441 ccaaaccatg tgtctaaaag ttgggaaaac cccacgaatc cgcaacatca gcaagcattt
    28501 gtcaatattc atgatgaaga gtacggtgta acagtcggaa actttggttt gaatgaatat
    28561 gaagtaacag aagacggaca aatcgcagtg acgctacttc gcagtgtggg agaactaggc
    28621 gattggggat attttccaac accagaagca cagtgtctag gtgagcatcg gttcaattac
    28681 agcattgaac tacatggacc agaagaaaaa ttctctactt atttgcatgc atacgcagca
    28741 cagattcctt tttccactca acaaatcaaa catcatgaag gaacattgat ttcaaaacaa
    28801 caatatttaa cgataaaaag tgaaacgttt gcaattacag ctttgaaacg cagcaagttt
    28861 agtgataaag tagtagtaag aggtttcaat atgtctagtc atttagaaaa attggaaata
    28921 actaaagaca atggaaaaac tgtcatattg aatctgcttg aagaaccaac gaagcaagcg
    28981 gttgtcccaa taatccaacc ttatgagatt cggacaatcg gattcgagga ggaaaacgaa
    29041 tgtatggcgg aatagaagca ggcggaacaa aatttgtgtg tgcggtttca gatggtggag
    29101 aaattttaga aaaattaagt atccctacca caacaccaga agaaacattg gatcaggtat
    29161 ttgaattttt tgatcgttat gaattgaagg gaatcgggat tggctcattc ggcccgattg
    29221 gtatcgacaa aacaaaagat aattacggat acgttttggc aactcccaaa aaagggtggg
    29281 cacaattcga ttttcttggc acaatcaagg aacgctacaa tattcctgct gcttggacaa
    29341 cggatgtcaa tgctgctgca tatggggaat tgaagcaagg agcagcacaa ggaaaaaata
    29401 gttgtattta ccttaccgtg ggaacaggaa tcggtgcagg tgtcgtgatc aatgaagaaa
    29461 ttttttcagg gatcgcacat cctgaaatgg gacatatttg ggtgaaacgc catccagatg
    29521 accattatga aggtacttgt ccttatcata aagattgttt agaaggatta gcagcaggac
    29581 cgtctattga ggcacgcaca gggatcaaag ggcaagatct gccgcaagat catcctgttt
    29641 gggctattca ggcttattat attgctcaag cactgatcaa ctatacgttg acgttagcac
    29701 ctgaaaagat tattttaggc ggtggggtaa tgaatcagga tcatctcctt caaaaaatcc
    29761 gtcagcaatt tgttgaattg atgggtggtt atatggaaac acctcaagta agtgaatata
    29821 tcgttcgttg gggactgccg aatgaaagtg ggattatcgg tagtttgctg ttagcagaac
    29881 acgaacatca aaagtaagca gacaaattac acaaaaagaa cagagtctta tcattatctg
    29941 ctgataagac tctgttcttt tttatttcta ttcccataat ccaggtaaag ctttaccgac
    30001 tattttttct tgagtaaatg gatggatcat gtgcagttct aatgcatgga gcatcagtcg
    30061 ttcagctgtt ctattttggt agagagggtc tccaacgatc ggatgtccct tatgagctaa
    30121 atgaactctg atctggtgtg tacgtccggt ttccaaaaca caatagactt gtgtttgttc
    30181 agaaagtact ttgttgacag tgacatgtgt caaggcgctc tgaccatttt tatcatctac
    30241 gacccttttt ctccgatcat ggcgatctcg tccaatttta ttggtgatgg tcatatcttt
    30301 ggcaatttct ccccatacga tggcttgata tctccgatag atcatttttt gttccaacat
    30361 tcttccaagt atcggcaaga caaatggatt ttttgcaaat aaaacggcac cgcttgtttc
    30421 tttgtccaag cggtggacaa cgtaaggtcg ttgctgttta gtgtcgagat aagcagctaa
    30481 atgattgagt aaggtactgt tttctgtcgg ttcatttgga tgagtcttca ttcctgcagg
    30541 cttatccact ataatcaaat gctcatcttc atataaaaca cggatgtgct tcgcatctcc
    30601 taattgaact tcttgataat tataatctgt ttcttcgaat aacaatgtga tctggtcatt
    30661 gccctttact tcatgatgga acaaagcagg ttgttgattg atccaaacgt ttttacgtat
    30721 acgcaaaaag tgtcgaacct ttctaggaac taaccattcc ttttcaagca agtcgcgaat
    30781 tgttgtttca ggatggttca taggtaaagt gatcgtgatt ttcacaagtg tactcctttc
    30841 agtgcaattg cagactaatt gtaccatttt taaaacatat ttaagaaaaa acttacattt
    30901 ttctgttcat tcttatgtta ctctacttgc actaacgtaa aaaaacatat tgaactacaa
    30961 cttaagcagg aagaaaacac tctttcacta tggtaaaata agacgattga atcaaataga
    31021 caggagaaga agcaaatgga ttttcaaagc attttagaaa agataaaagc tgctttgatc
    31081 agtttttggc gttggatcag accatatctc ggacaattcc atcgatggcg aaagcgcatc
    31141 tggaaaaaat atcatgtgaa caaattgatt ttgctgattg gtttgatctg tgtcttagtg
    31201 acaagtatct acctctttat tttagcaaaa caagcaaatg tcgaaacgtt aaaatcagga
    31261 ttaagccaat cgacagtcat ttatgataag aaaaacgaag aagctggcac actgtacgcc
    31321 caaaaaggaa catatgtaga attaaacgcg atttctccct atatccaaga tgcagtaatc
    31381 tcgacagaag ataggaattt ttacgagcat cacgggtttg atatcaaagg gattgcccgt
    31441 gcagcagtcc gaatggtctt gtctggcgga acatcagggg gcggaggaag tacgatcact
    31501 cagcagcttg ccaaaaatgc gtatctgaca ttagaccaaa cgtttgatcg aaaagcgaag
    31561 gaattatttt tggcaatcga gatcgaaaag aagtacagca aagaagaaat cttgaccatg
    31621 tatcttaaca atgcctattt tggcaacggg gtatggggtg tccaagacgc ttccagaaag
    31681 tattttggag tagatgcaac gaatgttacg ctgtctgagg ctgctacttt ggcaggaatg
    31741 ctgaaaggtc ctggtattta taatccgatc gatcacccaa aaaatgcgaa tgatcggaaa
    31801 aacacagtac taagtgtaat ggaagaaaac ggaaaaatca caaaagaaca agaacaagca
    31861 gaaagccaga cagatatcag cgcgtatctt aatgacacat acgataattc agattctggt
    31921 tatcgttatc cttattattt tgatgcagtg atcgatgaag cagttaacga gtatggtctt
    31981 gatgaagaag atgtcatgaa taaaggctat aaaatctata cagctttaga tcaggattat
    32041 caaaagcaat tagaagctac ttatcaaaac agtgcattat tcccagcaaa tgcttcagat
    32101 ggtgccatgg tccaatctgc ttcagtagct ttagatccga atacaggtgg tgtacaagca
    32161 ctagtaggac gaagaggaga tcatgtcttt agaggattca gctttgctac acagatgcgt
    32221 cgttcaccag gatcaacgat caaaccatta agcgtatata caccagcact ggaagctgga
    32281 tacaagcctt ccagtatctt gaaagacgaa ccgcaaagtt attacgatgc gcataatttt
    32341 gacgggacat accaaggaga agtaccgatg tatcaagctg ttgcacaaag tttgaatttg
    32401 cctgctgtat ggcttttgca tgagatcggt ttacaaaaag gatatgataa agcagaagaa
    32461 ttcggcctgc ctttagcaaa atctgataag tattatggct tagcccttgg tggactggaa
    32521 aaaggagtat ctccattgat catggcgggt gcttacagtg catttgccaa tgatggtcag
    32581 atgtatacgc cgcatcttat cacgaaaatc gttgattcga ctggagcggt gatcgtcgat
    32641 aacacaaaca aaaaaccaaa acaggtcata tcaaaggaaa cagccgatga aatgaccagc
    32701 atgcttcttg gtacattttc aaatggaaca gcggctgctg cgaatcctgc aggttatacg
    32761 atagcaggga aaacaggaac cacagaaact aatttcgatg ccacaaaagc aaatgaccaa
    32821 tggatgatcg gttacacgcc agacgtagtg atttcgacat ggatgggtta cgaagaatcc
    32881 agcaagctgc actatttgga agggacaagt ggaaatgttg tcgggaaagt cttcaaatca
    32941 gcggcagaag gcatccttcc gtatacttca aaaacaggat ttagtgtagc agatgcctat
    33001 gccacaggag gacaagtggt agctgcagat caagttggaa acacaggaaa ttctaatgga
    33061 gagtctggaa attggcaaga taacttgaat caatatggtg aacaagcaaa agaagggctg
    33121 aaaaacttcg gcgatatggt gaaagaaggc gttcagggaa ttggtgaagc ggctaaagac
    33181 ctctggcgga aatatcaagg agagtagccc ctttgaaaac gaataacccc atgttacaat
    33241 agagagtaac tactaaaaga aaagagggca taacatgtca aacatctacg atagcgcgaa
    33301 tcaaatcgaa cgtgagatcc gtgaattacc agaattcaaa gcgttgaaag aagcgtacga
    33361 gaaagtacaa gcaaacgaag aagcacacaa actgttcaaa gacttccaag cgatgcaaat
    33421 cgaattacaa gaaaaacaaa tgagtggaca agagttcagc gacgaagatg caatgaaagc
    33481 acaagaaatg gctgtgaaaa tccaatcaga agaagtaatc aatgacttga tgcaaaaaga
    33541 gcaaggcttc agtacaatca tcaatgactt gaaccgtatc atcatgacac cagttcgcga
    33601 cctttacagc gaataaaact attttatgaa acaacagagg gtgtgaaaga gacgttcggc
    33661 cttgagcgat gacaaaattg cttcttattg ccgataggct gtctcttgaa caccatttac
    33721 caagaggtcg tgagcctacc gtttaactct gagttacaag gaacaatttc aaaaaacagc
    33781 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
    33841 attcagaaat aggagctacg acaaaagtct tgtcatagct cctatttttt gtcattcatt
    33901 tttcaagcgg tagaaatagc ctttttgagc taacagtttt tcgtaagtac ctatttcaac
    33961 gatttttcct tcatcgatca caacgatttt atctgcctct ttgatcgttg aaagccgatg
    34021 agccacaata aaagtagtgg gtgtttctgt caggtaattc agggcttgct ggattttttt
    34081 ctctgactgg ttatctaaag ctgaggttgc ttcatctagc aaaatgattt ttggctgacg
    34141 tatcaacgct cgtgcaatgg agatacattg cttttggcca ccagacagct tgttgccatg
    34201 ttcgcctact aaggtatcca gaccatcagg aagctggtca atcagtgaag aaagttgcgc
    34261 agcttcgatg acttcatcta aacgttcttt tgatacgttt ttcaagccat aagtgatgtt
    34321 ctctttgatc gaagcggaga acaacaaagt ggtttgagga acaacggcta aataatttcg
    34381 cacacttcgc atatccagtt catccaaagg ctgatcatct agaaaaattt ttcctgaagt
    34441 cggctggata aatccaatca ataagttaag gagtgtagat tttccagagc cagatggccc
    34501 aacaaaagca attgtttctc cagctttcac ctctagagaa aaatgatcga taatcggttt
    34561 gagtgagtcc ggataaagaa aagaaacatc ctcaaaacga aaatttccat ccaaatgttg
    34621 gatttttttc ttaccgtgat aagtttctac atcgccagca atcaacagtt cgccaataga
    34681 agtgatagat tccatgcctt ttgaaatcgt aggaatcagc gtgatcattc cagaaacgga
    34741 attgacgatc gtcgtgaaat acgtctgata catcacgaca tcgccgggtt ggatgcgccc
    34801 ttgtaaagct aatatcccag tgaaaatcaa acaaatcagc tgaaatatct gaaaactagc
    34861 ccagctgact gatccgaaaa cagattgcag gatatctaag tggaatcctt tttcatagat
    34921 ttgagtaaaa tgcttattta agcgagttgt ttcgtgtttt tctaacccat gagctcgagc
    34981 aatcggtgct aattcgatca cttcgatcac ttttgcagaa gtctcttcca tttcttgcct
    35041 gaagtctgag ttggactgtc tgatcctttt acggaaaaag agagtaacga atgcggcgag
    35101 aggaacagtc aataagaaaa acatgaaaac aacaggactt ttataaagcg tgatacctaa
    35161 agctacgaat aaattgacag cgatatttaa tccgctgaca aatacttgag aggacaacgt
    35221 ttggatagct tctacatcac ggatgatctt tgactggatt cgtccagatt gcaattcttt
    35281 ttgataagga atagatagtt cttggatttt cttgataagg gtcgctctta gtccagcttc
    35341 tacttgacgg atcgaagtgc tatggtattt tacatgaaaa taattcgtca atatattttg
    35401 accaatcaga caagccatca aaaaagcgtt gaaataaatg gcctgcaact gagaagaatc
    35461 tttcgatgaa gctgcattga ttacattggc aatagcgatc ggcattaacc aagatggaag
    35521 atgtttgata ataaaaaaga gtcctgacat ggcaaaacgc caataatttc cctgataaag
    35581 atgcagcaat gtctgaaaag catttgcatg atatttttga taacttttct gtattcgttc
    35641 ctccataatc ttctccttaa caatcttggt aaataaactg caagtgttct attgcttcag
    35701 gaacataaat ctcaggagta gcttcaagag tggcgtacat tagaggacgc tcatatttga
    35761 tataatgcag taccgggcgg aaatctaaac agcccttgcc aacaggtacc atgcggatct
    35821 ttccattttc catgatatag tctttgagat gaagcgctgt aatataacca tgtaaattgt
    35881 ccaaggcacc tcggatcact ttttcctgat cagcatgatt ttctttatgc atcaaattcg
    35941 cacaatctaa aatgattttt aaatttggtg aagctacttc atcgattaaa cgtttagcta
    36001 actgatagtt atagattgga tgattgatcc ctgcttcaac agcaacagtt acgccaaaat
    36061 tttctgcatg agcgactaat tcaatgatag aatttctggc gatctgataa gcttcttcag
    36121 taaaattttc ttttgtgtaa ccgacagaga cactaccggt ttccgtagca atcatcttgg
    36181 cttgataggc agagcacaga gcaatttgct gtttgaaagt tgccaatgct tgtgcacgaa
    36241 cttcttgatt tttgctggat aggttgatat agcatcctaa aatggaaagt tcgatattgt
    36301 ttttttggaa gtattcacca aaataggcag cactcccaag tgtcatgtca gttaatttaa
    36361 gttgttcagg aaatgctttt tgtggtgcaa attggatatg ggacaagtga agctgtgata
    36421 ctttttgaac gatttcttca cgatttcgaa catctaaatc gtgggcacgg atgcctaaat
    36481 ttaatggaaa catctcaaac tcctttttgt aaataattag caagaggcag acgggattct
    36541 tttatcaatt caacaattat tcgggcaact ttatcagcgc cttctggact aaagtgggta
    36601 ttgtctttta gtccttctgg gtaattttta caagtatcag gcataagatg caggtagaat
    36661 ttttttgtct cttcttttga gaaggtctgg aacaattctt gtgatcttga gaacacatct
    36721 aaaagaggaa tatggtgttt ctcagcaaat gcacgcatcg cttttggata gtctccaagt
    36781 atatctggat tcaatgttcc gttttctaag taatctcgtc gcgtcacaga tgtcagtaag
    36841 accatttgga cgttcgcttt ttttgcgcat tcaaaaaata tctgcagatt ttttaaatag
    36901 ctaccgaagg gaagcgtacc tctttcttct tggattttct ggtcgttatg tccaaattgt
    36961 acaaataaga aatcattcgg ctgaaactgt gaagctagac gggctaacct tccttcctgg
    37021 ataaatgatt tggtgctgcg tccattcttt gcttggttat tgatctggat ctcgggtgaa
    37081 aaataagcag agattttttc tccccaacct gtctcgggac gcttgttttc atctttttct
    37141 gccgcagtag aatcaccggc aatatggata gtaggcatgt tatctcttct ttcataaagg
    37201 tttataggca atgaagcaga acagcaagag aagaagacgg agcaa
//
LOCUS       DAJSBM010000021        34666 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJSBM010000021 DAJSBM010000000
VERSION     DAJSBM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.021"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ONT67_001266"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ONT67_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCR2355019.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ONT67_001267"
     CDS             complement(2020..2922)
                     /locus_tag="ONT67_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2355020.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ONT67_001268"
     CDS             complement(2919..3611)
                     /locus_tag="ONT67_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCR2355021.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ONT67_001269"
     CDS             complement(3654..4295)
                     /locus_tag="ONT67_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2355022.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="ONT67_001270"
     CDS             complement(4333..4602)
                     /locus_tag="ONT67_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355023.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="ONT67_001271"
     CDS             complement(4575..5264)
                     /locus_tag="ONT67_001271"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR2355024.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ONT67_001272"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ONT67_001272"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR2355025.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ONT67_001273"
     CDS             complement(5984..6916)
                     /locus_tag="ONT67_001273"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCR2355026.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ONT67_001274"
     CDS             complement(6913..7689)
                     /locus_tag="ONT67_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCR2355027.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ONT67_001275"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ONT67_001275"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR2355028.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="ONT67_001276"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="ONT67_001276"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR2355029.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ONT67_001277"
     CDS             complement(12014..13294)
                     /locus_tag="ONT67_001277"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR2355030.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ONT67_001278"
     CDS             complement(13313..14239)
                     /locus_tag="ONT67_001278"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCR2355031.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ONT67_001279"
     CDS             complement(14252..15535)
                     /locus_tag="ONT67_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR2355032.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ONT67_001280"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ONT67_001280"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCR2355033.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ONT67_001281"
     CDS             complement(16323..17234)
                     /locus_tag="ONT67_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2355034.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="ONT67_001282"
     CDS             complement(17231..17695)
                     /locus_tag="ONT67_001282"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR2355035.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ONT67_001283"
     CDS             complement(17700..18398)
                     /locus_tag="ONT67_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2355036.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ONT67_001284"
     CDS             complement(18385..18771)
                     /locus_tag="ONT67_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355037.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ONT67_001285"
     CDS             complement(18918..20588)
                     /locus_tag="ONT67_001285"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCR2355038.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ONT67_001286"
     CDS             20771..21004
                     /locus_tag="ONT67_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCR2355039.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ONT67_001287"
     CDS             complement(21103..21534)
                     /locus_tag="ONT67_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCR2355040.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ONT67_001288"
     CDS             21613..22026
                     /locus_tag="ONT67_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCR2355041.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ONT67_001289"
     CDS             complement(22064..23212)
                     /locus_tag="ONT67_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2355042.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ONT67_001290"
     CDS             complement(23334..23930)
                     /locus_tag="ONT67_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2355043.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ONT67_001291"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ONT67_001291"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR2355044.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ONT67_001292"
     CDS             27160..27591
                     /locus_tag="ONT67_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355045.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ONT67_001293"
     CDS             complement(27636..28985)
                     /locus_tag="ONT67_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR2355046.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ONT67_001294"
     CDS             complement(29055..30218)
                     /locus_tag="ONT67_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2355047.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ONT67_001295"
     CDS             30347..30670
                     /locus_tag="ONT67_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355048.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ONT67_001296"
     CDS             complement(30853..32202)
                     /locus_tag="ONT67_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355049.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ONT67_001297"
     CDS             complement(32271..33224)
                     /locus_tag="ONT67_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR2355050.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ONT67_001298"
     CDS             complement(33326..34516)
                     /locus_tag="ONT67_001298"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR2355051.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9847 a         7593 c         6088 g        11138 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat cttttt
//
LOCUS       DAJSBM010000022        32178 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJSBM010000022 DAJSBM010000000
VERSION     DAJSBM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.022"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(407..766)
                     /locus_tag="ONT67_001299"
     CDS             complement(407..766)
                     /locus_tag="ONT67_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR2355052.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="ONT67_001300"
     CDS             complement(796..1137)
                     /locus_tag="ONT67_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355053.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="ONT67_001301"
     CDS             complement(1134..1808)
                     /locus_tag="ONT67_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2355054.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT67_001302"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="ONT67_001302"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR2355055.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="ONT67_001303"
     CDS             complement(4322..5512)
                     /locus_tag="ONT67_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2355056.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="ONT67_001304"
     CDS             complement(5533..6060)
                     /locus_tag="ONT67_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCR2355057.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="ONT67_001305"
     CDS             complement(6107..8896)
                     /locus_tag="ONT67_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR2355058.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="ONT67_001306"
     CDS             complement(9043..9243)
                     /locus_tag="ONT67_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2355059.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="ONT67_001307"
     CDS             complement(9696..10016)
                     /locus_tag="ONT67_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355060.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="ONT67_001308"
     CDS             complement(10519..11637)
                     /locus_tag="ONT67_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355061.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="ONT67_001309"
     CDS             12108..13415
                     /locus_tag="ONT67_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCR2355062.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="ONT67_001310"
     CDS             13536..14735
                     /locus_tag="ONT67_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2355063.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="ONT67_001311"
     CDS             complement(14762..16030)
                     /locus_tag="ONT67_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCR2355064.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="ONT67_001312"
     CDS             complement(16200..16841)
                     /locus_tag="ONT67_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCR2355065.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="ONT67_001313"
     CDS             complement(17250..17753)
                     /locus_tag="ONT67_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2355066.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="ONT67_001314"
     CDS             complement(17876..18640)
                     /locus_tag="ONT67_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCR2355067.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="ONT67_001315"
     CDS             18748..19023
                     /locus_tag="ONT67_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR2355068.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="ONT67_001316"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="ONT67_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR2355069.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="ONT67_001317"
     CDS             complement(20579..20980)
                     /locus_tag="ONT67_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2355070.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="ONT67_001318"
     CDS             complement(20994..21803)
                     /locus_tag="ONT67_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2355071.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="ONT67_001319"
     CDS             complement(21790..22680)
                     /locus_tag="ONT67_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355072.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="ONT67_001320"
     CDS             complement(22693..23172)
                     /locus_tag="ONT67_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355073.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="ONT67_001321"
     CDS             complement(23348..24289)
                     /locus_tag="ONT67_001321"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCR2355074.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT67_001322"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="ONT67_001322"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2355075.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="ONT67_001323"
     CDS             complement(25274..26470)
                     /locus_tag="ONT67_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2355076.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="ONT67_001324"
     CDS             complement(26488..27216)
                     /locus_tag="ONT67_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2355077.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT67_001325"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="ONT67_001325"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCR2355078.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="ONT67_001326"
     CDS             complement(28628..29086)
                     /locus_tag="ONT67_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2355079.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="ONT67_001327"
     CDS             complement(29083..30546)
                     /locus_tag="ONT67_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2355080.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT67_001328"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="ONT67_001328"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR2355081.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="ONT67_001329"
     CDS             31602..>32178
                     /locus_tag="ONT67_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2355082.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DAJSBM010000023        31560 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJSBM010000023 DAJSBM010000000
VERSION     DAJSBM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.023"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            49..510
                     /gene="mscL"
                     /locus_tag="ONT67_001330"
     CDS             49..510
                     /gene="mscL"
                     /locus_tag="ONT67_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR2355083.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(570..1016)
                     /locus_tag="ONT67_001331"
     CDS             complement(570..1016)
                     /locus_tag="ONT67_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355084.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(1109..1354)
                     /locus_tag="ONT67_001332"
     CDS             complement(1109..1354)
                     /locus_tag="ONT67_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCR2355085.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(1470..2048)
                     /locus_tag="ONT67_001333"
     CDS             complement(1470..2048)
                     /locus_tag="ONT67_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2355086.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(2125..2655)
                     /locus_tag="ONT67_001334"
     CDS             complement(2125..2655)
                     /locus_tag="ONT67_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCR2355087.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT67_001335"
     CDS             complement(2659..3519)
                     /gene="rlmB"
                     /locus_tag="ONT67_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR2355088.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(3512..3907)
                     /locus_tag="ONT67_001336"
     CDS             complement(3512..3907)
                     /locus_tag="ONT67_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCR2355089.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(3904..5316)
                     /locus_tag="ONT67_001337"
     CDS             complement(3904..5316)
                     /locus_tag="ONT67_001337"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR2355090.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT67_001338"
     CDS             complement(5313..5849)
                     /gene="cysE"
                     /locus_tag="ONT67_001338"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR2355091.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(5935..6174)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6171..7631)
                     /locus_tag="ONT67_001339"
     CDS             complement(6171..7631)
                     /locus_tag="ONT67_001339"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR2355092.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(7801..8958)
                     /locus_tag="ONT67_001340"
     CDS             complement(7801..8958)
                     /locus_tag="ONT67_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCR2355093.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT67_001341"
     CDS             complement(8992..10362)
                     /gene="radA"
                     /locus_tag="ONT67_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR2355094.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(10425..10952)
                     /locus_tag="ONT67_001342"
     CDS             complement(10425..10952)
                     /locus_tag="ONT67_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR2355095.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            11182..11988
                     /gene="proB"
                     /locus_tag="ONT67_001343"
     CDS             11182..11988
                     /gene="proB"
                     /locus_tag="ONT67_001343"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR2355096.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            11985..13232
                     /locus_tag="ONT67_001344"
     CDS             11985..13232
                     /locus_tag="ONT67_001344"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR2355097.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            13308..13652
                     /locus_tag="ONT67_001345"
     CDS             13308..13652
                     /locus_tag="ONT67_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355098.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(13769..14095)
                     /locus_tag="ONT67_001346"
     CDS             complement(13769..14095)
                     /locus_tag="ONT67_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355099.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(14710..15912)
                     /locus_tag="ONT67_001347"
     CDS             complement(14710..15912)
                     /locus_tag="ONT67_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355100.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            16029..16544
                     /locus_tag="ONT67_001348"
     CDS             16029..16544
                     /locus_tag="ONT67_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2355101.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(16743..17189)
                     /locus_tag="ONT67_001349"
     CDS             complement(16743..17189)
                     /locus_tag="ONT67_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2355102.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT67_001350"
     CDS             complement(17236..18714)
                     /gene="msr(C)"
                     /locus_tag="ONT67_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2355103.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(19068..19697)
                     /locus_tag="ONT67_001351"
     CDS             complement(19068..19697)
                     /locus_tag="ONT67_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2355104.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(19681..20145)
                     /locus_tag="ONT67_001352"
     CDS             complement(19681..20145)
                     /locus_tag="ONT67_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2355105.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(20632..21900)
                     /locus_tag="ONT67_001353"
     CDS             complement(20632..21900)
                     /locus_tag="ONT67_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2355106.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(22131..22868)
                     /locus_tag="ONT67_001354"
     CDS             complement(22131..22868)
                     /locus_tag="ONT67_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2355107.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(22935..23504)
                     /locus_tag="ONT67_001355"
     CDS             complement(22935..23504)
                     /locus_tag="ONT67_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2355108.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            23632..24504
                     /locus_tag="ONT67_001356"
     CDS             23632..24504
                     /locus_tag="ONT67_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2355109.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            24846..25718
                     /locus_tag="ONT67_001357"
     CDS             24846..25718
                     /locus_tag="ONT67_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2355110.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            25715..27673
                     /locus_tag="ONT67_001358"
     CDS             25715..27673
                     /locus_tag="ONT67_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2355111.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(28005..28856)
                     /locus_tag="ONT67_001359"
     CDS             complement(28005..28856)
                     /locus_tag="ONT67_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2355112.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            28979..29446
                     /locus_tag="ONT67_001360"
     CDS             28979..29446
                     /locus_tag="ONT67_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCR2355113.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(29709..30191)
                     /locus_tag="ONT67_001361"
     CDS             complement(29709..30191)
                     /locus_tag="ONT67_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCR2355114.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(30291..31154)
                     /locus_tag="ONT67_001362"
     CDS             complement(30291..31154)
                     /locus_tag="ONT67_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2355115.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9033 a         6670 c         5709 g        10148 t
ORIGIN      
        1 tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa
       61 ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt
      121 agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt
      181 tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac
      241 tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca
      301 tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat
      361 ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt
      421 agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag
      481 caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag aaagccgatt
      541 ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt tttcatgtcg
      601 atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc aaacgactca
      661 taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg aaaatttttc
      721 tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt acggttgtcc
      781 tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac tcgcgtgtag
      841 gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc atcaatgtca
      901 tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg ttctttgacg
      961 atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata ttccactaag
     1021 caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa agcaaacgaa
     1081 ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat tcgatctcaa
     1141 ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa ttttcttcag
     1201 gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg cggcgttctg
     1261 tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata cgaccttcta
     1321 aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact atcaactata
     1381 ccacatattt ttaaaatttt caagtttacc ataaaaaaaa aaagaaatgc aataaaaata
     1441 aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt
     1501 tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa
     1561 cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct
     1621 atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt
     1681 gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt
     1741 tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac
     1801 agagaataga tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg
     1861 cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc
     1921 tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat
     1981 ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc
     2041 tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt
     2101 catcgaactg attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg
     2161 ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg
     2221 catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc
     2281 ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct
     2341 tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata
     2401 actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt
     2461 ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata
     2521 tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg
     2581 ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa
     2641 cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa
     2701 accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa
     2761 ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac caatgactaa
     2821 agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa
     2881 tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc
     2941 aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac
     3001 tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat
     3061 cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt
     3121 tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc
     3181 aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac
     3241 ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc
     3301 tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc
     3361 ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga
     3421 ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc
     3481 acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa
     3541 tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca
     3601 tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg
     3661 tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat
     3721 gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg
     3781 ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat
     3841 agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat
     3901 ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt
     3961 ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg
     4021 atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa
     4081 aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc
     4141 agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt
     4201 gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt
     4261 ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg
     4321 gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat
     4381 cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa
     4441 gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt
     4501 actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc
     4561 gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc
     4621 taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac
     4681 ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc
     4741 gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc
     4801 ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt
     4861 cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata
     4921 gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct
     4981 tggatgcagt gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat
     5041 gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt
     5101 atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata
     5161 gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata
     5221 cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc
     5281 tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct
     5341 ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc
     5401 aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc
     5461 tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct
     5521 tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg
     5581 gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct
     5641 cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat
     5701 aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa
     5761 ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct
     5821 gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg
     5881 taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa
     5941 gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg
     6001 cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt
     6061 ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc
     6121 ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt
     6181 acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat
     6241 tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct
     6301 tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact
     6361 acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca
     6421 gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac
     6481 agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc
     6541 acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa
     6601 ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc
     6661 cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac
     6721 attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat
     6781 aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata
     6841 aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc
     6901 aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt
     6961 tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga
     7021 tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag
     7081 tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg
     7141 tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt
     7201 tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct
     7261 cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat
     7321 gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat
     7381 tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac
     7441 caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta
     7501 tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat
     7561 gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt
     7621 actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg
     7681 tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt
     7741 ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt
     7801 ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc
     7861 atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac
     7921 gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc
     7981 atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat
     8041 cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac
     8101 aggtacattt tggaattcag agactttatt caagttatag tcattggtta caatgacacc
     8161 atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc
     8221 tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa
     8281 tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc
     8341 gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc
     8401 ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc
     8461 tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt
     8521 tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa
     8581 tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat
     8641 ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc
     8701 gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt
     8761 gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc
     8821 taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg
     8881 ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt
     8941 aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa
     9001 cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc
     9061 ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac
     9121 ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc
     9181 accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac
     9241 agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta
     9301 agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga
     9361 tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt
     9421 gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc
     9481 agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc
     9541 ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt
     9601 ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt
     9661 atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc
     9721 gacgataaaa atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc
     9781 tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat
     9841 ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat
     9901 atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa
     9961 ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc
    10021 taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat
    10081 caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt
    10141 tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt
    10201 ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac
    10261 catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc
    10321 gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat
    10381 cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa
    10441 ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa
    10501 aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg
    10561 gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc
    10621 acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca
    10681 agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt
    10741 ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac
    10801 ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa
    10861 tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat
    10921 ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc
    10981 tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc
    11041 tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa
    11101 actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg
    11161 gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc
    11221 aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat
    11281 cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca
    11341 tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt
    11401 cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa
    11461 agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat
    11521 tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg
    11581 atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa
    11641 tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg
    11701 atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct
    11761 cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct
    11821 ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt
    11881 gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt
    11941 cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc
    12001 aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca
    12061 aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg
    12121 aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag
    12181 accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg
    12241 cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga
    12301 gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa
    12361 atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg
    12421 gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg
    12481 tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg
    12541 caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa
    12601 aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa
    12661 ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca
    12721 atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg
    12781 agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt
    12841 tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag
    12901 attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg
    12961 aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata
    13021 gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg
    13081 cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta
    13141 ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata
    13201 tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct
    13261 tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt
    13321 taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta
    13381 ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt
    13441 cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca
    13501 tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg
    13561 cttatacgat tacgctactg attgctggga tttttcactt atcttataaa tatcagctga
    13621 ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc
    13681 ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag
    13741 cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt
    13801 cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca
    13861 tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc
    13921 gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg
    13981 cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt
    14041 caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt
    14101 atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc
    14161 attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat
    14221 aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa
    14281 actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg
    14341 gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga
    14401 agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct
    14461 gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
    14521 gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc
    14581 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
    14641 gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc
    14701 tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca
    14761 tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc
    14821 accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata
    14881 gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag
    14941 tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg
    15001 catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa
    15061 aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat
    15121 agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat
    15181 caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat
    15241 tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac
    15301 ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa
    15361 catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc
    15421 gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt
    15481 ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc
    15541 attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc
    15601 gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc
    15661 gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact
    15721 agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa
    15781 gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag
    15841 cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc
    15901 caatatttcc atccctttct ctctattagc atcatacatg atttatttta catgtcaata
    15961 tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta
    16021 ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt
    16081 ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct
    16141 atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc
    16201 actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga
    16261 tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt
    16321 tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa caacagctga
    16381 acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag
    16441 aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat
    16501 ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct
    16561 ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa
    16621 agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa
    16681 ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc
    16741 catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc
    16801 aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat
    16861 aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt
    16921 cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact
    16981 ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa
    17041 gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc
    17101 agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa
    17161 attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa
    17221 atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat
    17281 ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat
    17341 cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa
    17401 attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt
    17461 cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc
    17521 aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat
    17581 gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg
    17641 aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa
    17701 ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg
    17761 aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat
    17821 gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat
    17881 ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt
    17941 cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct
    18001 gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt
    18061 ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag
    18121 ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc
    18181 tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg
    18241 atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc
    18301 aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga
    18361 agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga
    18421 agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc
    18481 agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg
    18541 atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc
    18601 tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag
    18661 ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt
    18721 tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg
    18781 tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc
    18841 accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca
    18901 tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc
    18961 gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca
    19021 cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata
    19081 gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt
    19141 gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga
    19201 tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg
    19261 aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa
    19321 aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc
    19381 ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg
    19441 ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa
    19501 ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata
    19561 ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg
    19621 attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa
    19681 tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg
    19741 ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg
    19801 ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc
    19861 tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac
    19921 atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa
    19981 cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc
    20041 gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc
    20101 taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct
    20161 aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa
    20221 agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt
    20281 tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga
    20341 ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat
    20401 ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta
    20461 aacagtaata aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt
    20521 caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt
    20581 gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc
    20641 ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc
    20701 actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg
    20761 cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga
    20821 gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg
    20881 gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac
    20941 agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg
    21001 gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt
    21061 ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc
    21121 tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata
    21181 gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga
    21241 ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac
    21301 atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag
    21361 attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg
    21421 agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc
    21481 aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc
    21541 agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc
    21601 tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt
    21661 actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt
    21721 attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc
    21781 tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt
    21841 gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat
    21901 cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa
    21961 agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct
    22021 gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt
    22081 ttgaataact atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc
    22141 tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca
    22201 aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga
    22261 agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct
    22321 ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg
    22381 attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt
    22441 cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt
    22501 acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca
    22561 tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca
    22621 tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct
    22681 cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt
    22741 aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc
    22801 gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa
    22861 tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta
    22921 tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct
    22981 gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca
    23041 ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc
    23101 ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc
    23161 accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct
    23221 aagtcaacga acatcacttg acgattgata aagacatttt tccctatact aatatgtttg
    23281 ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg
    23341 atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct
    23401 aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact
    23461 cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt
    23521 atccttactt tataacggac ggcttatgat gtctaatact tataaaacat acttaactat
    23581 tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt
    23641 cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa
    23701 tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt
    23761 ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat
    23821 ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca
    23881 aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct
    23941 ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta
    24001 tatagcggga atgcagatga tatcatagac aaactggata gcggattact tgattttgga
    24061 ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat
    24121 cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg
    24181 caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta
    24241 gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg
    24301 tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc
    24361 atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca
    24421 ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat
    24481 gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca
    24541 acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct
    24601 tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa
    24661 cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca
    24721 tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt
    24781 tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg
    24841 cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac
    24901 aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt
    24961 ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa
    25021 atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg
    25081 cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca
    25141 ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag
    25201 aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga
    25261 caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc
    25321 acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat
    25381 ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt
    25441 cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc
    25501 aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa
    25561 gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca
    25621 cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac
    25681 aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg
    25741 acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg
    25801 aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa
    25861 cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt
    25921 ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt
    25981 tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc
    26041 ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct
    26101 ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg
    26161 tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt
    26221 atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg
    26281 gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca
    26341 agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt
    26401 ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat
    26461 gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa
    26521 agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac
    26581 agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat
    26641 tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt
    26701 tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt
    26761 gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc
    26821 tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga
    26881 tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc
    26941 attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca
    27001 agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat
    27061 gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc
    27121 agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt
    27181 ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa
    27241 tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg
    27301 tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat
    27361 cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt
    27421 cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt
    27481 aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat
    27541 tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca
    27601 aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc
    27661 caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg
    27721 agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc
    27781 cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct
    27841 tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt
    27901 ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
    27961 ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat
    28021 atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca
    28081 tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca
    28141 gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct
    28201 tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag
    28261 tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt
    28321 gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca
    28381 taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac
    28441 caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat
    28501 tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg
    28561 atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga
    28621 tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt
    28681 gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct
    28741 cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag
    28801 aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt
    28861 gccactccct attcttttta tttgttaaga tttacagctg tacatttata aattacacct
    28921 ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat
    28981 gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca
    29041 agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa
    29101 tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa
    29161 accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag
    29221 catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt
    29281 tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga
    29341 agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa
    29401 agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg
    29461 cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag
    29521 ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc
    29581 ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg
    29641 gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct
    29701 ctatattatc atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg
    29761 gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct
    29821 cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct
    29881 gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt
    29941 ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg
    30001 ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta
    30061 ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg
    30121 ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta
    30181 tactttgcca tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag
    30241 ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt
    30301 gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta
    30361 aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag
    30421 attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc
    30481 caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta
    30541 gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca
    30601 taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt
    30661 tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg
    30721 acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat
    30781 tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt
    30841 atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa
    30901 aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct
    30961 aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc
    31021 cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct
    31081 attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc
    31141 agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa
    31201 gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc
    31261 taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg
    31321 ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa
    31381 aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt
    31441 tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta
    31501 tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa
//
LOCUS       DAJSBM010000024        31245 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJSBM010000024 DAJSBM010000000
VERSION     DAJSBM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.024"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            202..1077
                     /locus_tag="ONT67_001363"
     CDS             202..1077
                     /locus_tag="ONT67_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355116.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="ONT67_001364"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="ONT67_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="ONT67_001365"
     CDS             complement(1642..2973)
                     /locus_tag="ONT67_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355117.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="ONT67_001366"
     CDS             complement(3122..3835)
                     /locus_tag="ONT67_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2355118.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="ONT67_001367"
     CDS             4010..5434
                     /locus_tag="ONT67_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2355119.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="ONT67_001368"
     CDS             5733..6440
                     /locus_tag="ONT67_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2355120.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="ONT67_001369"
     CDS             6427..6750
                     /locus_tag="ONT67_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2355121.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="ONT67_001370"
     CDS             complement(6839..7876)
                     /locus_tag="ONT67_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2355122.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="ONT67_001371"
     CDS             complement(8197..9180)
                     /locus_tag="ONT67_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCR2355123.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="ONT67_001372"
     CDS             complement(9595..10365)
                     /locus_tag="ONT67_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355124.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="ONT67_001373"
     CDS             complement(10358..11293)
                     /locus_tag="ONT67_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355125.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="ONT67_001374"
     CDS             complement(11609..13258)
                     /locus_tag="ONT67_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2355126.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="ONT67_001375"
     CDS             complement(13375..15978)
                     /locus_tag="ONT67_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR2355127.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="ONT67_001376"
     CDS             complement(15990..17039)
                     /locus_tag="ONT67_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR2355128.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="ONT67_001377"
     CDS             complement(17065..17379)
                     /locus_tag="ONT67_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR2355129.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="ONT67_001378"
     CDS             complement(17395..17841)
                     /locus_tag="ONT67_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2355130.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="ONT67_001379"
     CDS             complement(17842..19764)
                     /locus_tag="ONT67_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCR2355131.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="ONT67_001380"
     CDS             complement(19976..20872)
                     /locus_tag="ONT67_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2355132.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="ONT67_001381"
     CDS             complement(20865..21545)
                     /locus_tag="ONT67_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355133.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="ONT67_001382"
     CDS             complement(21546..22328)
                     /locus_tag="ONT67_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355134.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="ONT67_001383"
     CDS             complement(22321..23238)
                     /locus_tag="ONT67_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2355135.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="ONT67_001384"
     CDS             complement(23402..23725)
                     /locus_tag="ONT67_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355136.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="ONT67_001385"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="ONT67_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCR2355137.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="ONT67_001386"
     CDS             27853..28167
                     /locus_tag="ONT67_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2355138.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="ONT67_001387"
     CDS             28180..28554
                     /locus_tag="ONT67_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2355139.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="ONT67_001388"
     CDS             28564..28899
                     /locus_tag="ONT67_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCR2355140.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="ONT67_001389"
     CDS             28982..30322
                     /locus_tag="ONT67_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2355141.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="ONT67_001390"
     CDS             30400..31074
                     /locus_tag="ONT67_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCR2355142.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="ONT67_001391"
     CDS             31067..31231
                     /locus_tag="ONT67_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355143.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
BASE COUNT         9736 a         6069 c         5515 g         9925 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaat
//
LOCUS       DAJSBM010000025        29981 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJSBM010000025 DAJSBM010000000
VERSION     DAJSBM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.025"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            137..475
                     /locus_tag="ONT67_001392"
     CDS             137..475
                     /locus_tag="ONT67_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355144.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(680..1255)
                     /locus_tag="ONT67_001393"
     CDS             complement(680..1255)
                     /locus_tag="ONT67_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCR2355145.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1987..2568)
                     /locus_tag="ONT67_001394"
     CDS             complement(1987..2568)
                     /locus_tag="ONT67_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2355146.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2751..3377)
                     /locus_tag="ONT67_001395"
     CDS             complement(2751..3377)
                     /locus_tag="ONT67_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2355147.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3399..4730)
                     /locus_tag="ONT67_001396"
     CDS             complement(3399..4730)
                     /locus_tag="ONT67_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCR2355148.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4885..5286)
                     /locus_tag="ONT67_001397"
     CDS             complement(4885..5286)
                     /locus_tag="ONT67_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2355149.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5372..5578)
                     /locus_tag="ONT67_001398"
     CDS             complement(5372..5578)
                     /locus_tag="ONT67_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355150.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5673..7175)
                     /locus_tag="ONT67_001399"
     CDS             complement(5673..7175)
                     /locus_tag="ONT67_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCR2355151.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT67_001400"
     CDS             complement(7193..7648)
                     /gene="argR"
                     /locus_tag="ONT67_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCR2355152.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7746..8363)
                     /locus_tag="ONT67_001401"
     CDS             complement(7746..8363)
                     /locus_tag="ONT67_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR2355153.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8584..9957
                     /locus_tag="ONT67_001402"
     CDS             8584..9957
                     /locus_tag="ONT67_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355154.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10032..11144
                     /locus_tag="ONT67_001403"
     CDS             10032..11144
                     /locus_tag="ONT67_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR2355155.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11216..12853)
                     /locus_tag="ONT67_001404"
     CDS             complement(11216..12853)
                     /locus_tag="ONT67_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR2355156.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13021..13629)
                     /locus_tag="ONT67_001405"
     CDS             complement(13021..13629)
                     /locus_tag="ONT67_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2355157.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT67_001406"
     CDS             complement(14013..15164)
                     /gene="queG"
                     /locus_tag="ONT67_001406"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR2355158.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15183..15935)
                     /locus_tag="ONT67_001407"
     CDS             complement(15183..15935)
                     /locus_tag="ONT67_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355159.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16076..17026)
                     /locus_tag="ONT67_001408"
     CDS             complement(16076..17026)
                     /locus_tag="ONT67_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCR2355160.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17027..17866)
                     /locus_tag="ONT67_001409"
     CDS             complement(17027..17866)
                     /locus_tag="ONT67_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCR2355161.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17863..18609)
                     /locus_tag="ONT67_001410"
     CDS             complement(17863..18609)
                     /locus_tag="ONT67_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCR2355162.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18847..20217
                     /locus_tag="ONT67_001411"
     CDS             18847..20217
                     /locus_tag="ONT67_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355163.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20272..21168)
                     /locus_tag="ONT67_001412"
     CDS             complement(20272..21168)
                     /locus_tag="ONT67_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2355164.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21282..21452)
                     /locus_tag="ONT67_001413"
     CDS             complement(21282..21452)
                     /locus_tag="ONT67_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355165.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(21449..22126)
                     /locus_tag="ONT67_001414"
     CDS             complement(21449..22126)
                     /locus_tag="ONT67_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2355166.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22217..23122)
                     /locus_tag="ONT67_001415"
     CDS             complement(22217..23122)
                     /locus_tag="ONT67_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR2355167.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23297..24751
                     /locus_tag="ONT67_001416"
     CDS             23297..24751
                     /locus_tag="ONT67_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355168.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            24767..25798
                     /locus_tag="ONT67_001417"
     CDS             24767..25798
                     /locus_tag="ONT67_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCR2355169.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25805..26992
                     /locus_tag="ONT67_001418"
     CDS             25805..26992
                     /locus_tag="ONT67_001418"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR2355170.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27148..28530
                     /locus_tag="ONT67_001419"
     CDS             27148..28530
                     /locus_tag="ONT67_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2355171.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28502..28888
                     /locus_tag="ONT67_001420"
     CDS             28502..28888
                     /locus_tag="ONT67_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355172.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29088..29756)
                     /locus_tag="ONT67_001421"
     CDS             complement(29088..29756)
                     /locus_tag="ONT67_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR2355173.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         8404 a         6140 c         5483 g         9954 t
ORIGIN      
        1 taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca aatactatag
       61 ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa tttcaaagac
      121 aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct tttgctctga
      181 caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa ctgttcggat
      241 caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta gacaagactt
      301 tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt tacatgatcc
      361 aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag aatatacaac
      421 tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt cataatgata
      481 aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa atagaagcta
      541 tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta tgagcatttc
      601 ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt gccgctcttt
      661 ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc tgctgagtaa
      721 tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct tcagtcaaga
      781 caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta gtcattatga
      841 ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata tacactattt
      901 tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcataa aagccactca
      961 ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt tcttctactg
     1021 tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat ccccacttca
     1081 tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca agatttgacg
     1141 gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc tgggcttctc
     1201 gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca cacatcctaa
     1261 tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc aacaaaacgc
     1321 tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca gtgaatggta
     1381 ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag aagctgcttt
     1441 ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct gggtaaacgg
     1501 tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     1561 ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct cttccttaaa
     1621 cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa tatgagcagc
     1681 tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca acctctccac
     1741 taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa aatatgagga
     1801 actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca caacctctaa
     1861 tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac aaaaatttga
     1921 gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
     1981 taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt gaaaattctg
     2041 cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct ggatttcagc
     2101 aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt cagaaagtgc
     2161 caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta gcaacagacg
     2221 gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac agccatgata
     2281 agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta atgcttcttc
     2341 tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata cgtccatatg
     2401 cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat aaaattgacc
     2461 ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat agcctttttt
     2521 ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg atattctcct
     2581 ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct tcttaatcgt
     2641 ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg ttctcatata
     2701 gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc ttatgatagt
     2761 tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa gttcgtcaac
     2821 gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac cacaacattg
     2881 aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc gttcattcca
     2941 ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc attgctttct
     3001 ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata aggtttcaat
     3061 tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact ttttctagca
     3121 aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca tttatggtca
     3181 tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt tgcttgtttt
     3241 accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt tgtcaaacca
     3301 aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac tactgctgta
     3361 tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac atcatttaga
     3421 gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata aggaagtttt
     3481 ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc atagatactt
     3541 gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc tttaggatct
     3601 tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt gtaattgacg
     3661 ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg agtgataaat
     3721 acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc cttcaactga
     3781 tccataatga ttcgataatc atctagagag atataagtga actgcaatcc gcctttgacg
     3841 tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac agcgattgct
     3901 ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt acttggtttt
     3961 cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc atcttctgta
     4021 aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac acctagatgg
     4081 aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc ttcatcttca
     4141 cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta ttcattgaac
     4201 attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt ttccggtaat
     4261 tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt caatcctttg
     4321 attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc ttcaacaatc
     4381 actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg tgctgtaccg
     4441 tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc gcgaagcatg
     4501 cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct ttctccattg
     4561 aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc atacatacgt
     4621 gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt cgctaaagtc
     4681 ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat ttttccctcg
     4741 cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt ctattctcca
     4801 aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc acgtttttag
     4861 tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga tatttcctct
     4921 agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt gggcttcttc
     4981 ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc cgccatcatc
     5041 taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc ctgcttcttt
     5101 gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa ataattgttc
     5161 tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca tcgagccatt
     5221 tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt cgtatgcttt
     5281 tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt atagtgtatg
     5341 acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg ccgtcttcta
     5401 ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata tttgttccaa
     5461 aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga tacatatata
     5521 ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc gttaccattt
     5581 tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac tttttttgcc
     5641 gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga ttcaagatca
     5701 gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt tccgaattca
     5761 tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat caaaaaccag
     5821 ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc cgggtcgaag
     5881 ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt gtttccttgt
     5941 tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac gggctgtttc
     6001 aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg caaaagcatc
     6061 aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt gaatgcagcg
     6121 tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag ttctaatgct
     6181 ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc tttttcttca
     6241 atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt tcctggatgc
     6301 tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat cactgttcca
     6361 gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc aagatctgct
     6421 tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc aggatttggg
     6481 atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga atccgcacct
     6541 aagctattca atactttttc agcaaagaat ccaccagcac catttccggc atcaacaatg
     6601 attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt aatttttgtg
     6661 actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc agtttgttcg
     6721 ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc tgctcctcct
     6781 tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc agtcagcata
     6841 accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc aggcgtagta
     6901 gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat caacgcctct
     6961 ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca ttttttattt
     7021 ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc gatctttttt
     7081 accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat tcctcgaata
     7141 tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc atttattccc
     7201 tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg caaataataa
     7261 tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact tcatctagcc
     7321 aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg aactgaacag
     7381 actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca tttaaagaaa
     7441 cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga ctttttacta
     7501 acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg cctgcttcat
     7561 gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg atctggcgga
     7621 tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga ttatagcaaa
     7681 agataagtaa tatttcttca tattttggta ttttttactt ttattttttg ttttcattca
     7741 ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc aggtgctgat
     7801 tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc atagccttct
     7861 tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata gggcccgacg
     7921 tgtaagattt gggcacaaag accttcttcc atctttttga atactagttg ttctttcaag
     7981 tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc tgtaacaaag
     8041 tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac tggttcatgc
     8101 aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata catctgataa
     8161 tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac tgcatataga
     8221 caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct gatgctaaag
     8281 aatggttttt taggaacctc tagtatttgc gggctttttt tcatactata gctcttgtct
     8341 ttacgccatt caattttttc catttttctt cctccgcgaa attccattta gttatttatt
     8401 atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc agtttgtgct
     8461 agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg agagagaggc
     8521 ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt agaggtgatg
     8581 taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac agggatgatg
     8641 tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac tcttatcaga
     8701 cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct tttgatgatt
     8761 tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa aaaactcttt
     8821 ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc tcctaccttc
     8881 atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc tcttccgttg
     8941 atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat gatgggtgtc
     9001 ggtactttga caacggccat tgctccagca attggcccca cttatggagg cattatgact
     9061 tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgat tctttctttg
     9121 attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta tttggattgg
     9181 ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt gagtacgttg
     9241 ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt ccttttttac
     9301 tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc aaataaaaca
     9361 ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg tgtatctttt
     9421 gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc tggattagcg
     9481 atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa aatgctggat
     9541 caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat tggttggcta
     9601 gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg tcatgtgttt
     9661 tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat gaatgcactg
     9721 aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca gttttctggc
     9781 gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca tacttcccat
     9841 aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt gttgctgttg
     9901 cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa agcgtagatt
     9961 ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg tttgtaaaag
    10021 gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt atgactctag
    10081 ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg acaggacagg
    10141 caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga aacaaacaat
    10201 ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa gatttccaac
    10261 ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa aaagatttag
    10321 ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt ggaaacatcg
    10381 aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca gcagtttcac
    10441 tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat cttggtcagc
    10501 gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat aagaagagcg
    10561 attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta attttgacac
    10621 ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat atacgtccgc
    10681 aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac tggcctgttg
    10741 ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc cttttaggtg
    10801 ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct tataagggat
    10861 tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac tggccatacg
    10921 agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc ggcgtactgc
    10981 cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg accacaggtc
    11041 cttatatcgg ctatctatta gccaacttct taaatactgg tcattggtct acggaagatt
    11101 atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac agctttttca
    11161 agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg ttattttagt
    11221 tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag agatcttttc
    11281 ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc agatttttat
    11341 aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg atgtcttcaa
    11401 atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg attgacaatg
    11461 tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca tagcaggctg
    11521 tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga ggatagacac
    11581 ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt gtagtgatat
    11641 tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct aaaactgggt
    11701 cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa acagcatcga
    11761 tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg actgtccgcc
    11821 catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt gtacatgtca
    11881 cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac ggatagtctt
    11941 gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt cgagaaataa
    12001 cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt gctaaggtaa
    12061 tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc tggtcattac
    12121 tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt cccaatgccc
    12181 aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct agcacagccg
    12241 ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca tgtgtccatt
    12301 cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt tgataagtct
    12361 ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa ttgttctcaa
    12421 atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg ccaacgatta
    12481 cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt aaaattttct
    12541 cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt gctgttcttc
    12601 ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa tctagttctt
    12661 cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt gctagttgat
    12721 aagctgtctt cctgtcaaaa acttcatgaa cattattacc gtaaaatgtc gtttctacaa
    12781 tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat tcgagactct
    12841 cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct ttattataca
    12901 ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta tttttcacac
    12961 aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc ttttatcggc
    13021 ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac cataacgaga
    13081 tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc cgtttaagta
    13141 tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt atgaaccgcc
    13201 tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg ttgcagcagc
    13261 tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat gttcttgcgt
    13321 tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg ttgctgaaga
    13381 atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga tgtttgcgat
    13441 cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt tttgtgagat
    13501 cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag catttgtagt
    13561 tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa ttttcttaaa
    13621 tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc tattaacttt
    13681 atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa aataacgaat
    13741 tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat aaaagccttg
    13801 ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa aaacgcagat
    13861 tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg atttttatca
    13921 aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca acaattactg
    13981 gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg caataatttc
    14041 tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa tatttcatct
    14101 aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc ccaagctgct
    14161 gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg caatgccgtt
    14221 cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt cttcccacgc
    14281 catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat cgaaagcatt
    14341 ggctttaatt ttggatatac ttcgtctact tttggttcca tctcttcatg aaaatgaaaa
    14401 tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata aatgacattt
    14461 cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg ataagacaaa
    14521 catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca agcagtcacg
    14581 cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa tacaatatta
    14641 gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa caatccgttt
    14701 cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag ttctccagta
    14761 tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc tcttgtttgg
    14821 atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc gatcccccaa
    14881 gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg gatcctagta
    14941 ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat ttctggataa
    15001 agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc tttttgttct
    15061 tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat tttgtcaatt
    15121 cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt cctcgcttgc
    15181 tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac aaactggatc
    15241 tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa tacttcccaa
    15301 taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt caatgaaact
    15361 ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa ctcagacaga
    15421 tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt gaatgtttgc
    15481 gggattttcc caggcctagc aatttctact agacgattat aaaacatatt cattaatttg
    15541 ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat tactccttct
    15601 gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc gttttgttta
    15661 actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc gataccggta
    15721 ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag ctcttcatct
    15781 ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc ttcaagaaat
    15841 tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa gacttcttct
    15901 ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt ccttactcta
    15961 actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca taattttttt
    16021 tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc acttttcagc
    16081 tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc atcatctgat
    16141 agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt ttatcataga
    16201 ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca ctggtttcca
    16261 cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc atttgatcag
    16321 gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa tcatatgctc
    16381 gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc tcttcgatag
    16441 aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca tatgcttctg
    16501 catttttttt atagagtgtc gcattttctg catctttttc agagaatata cgtgcgattt
    16561 cctccacata ttttattccg ttagataggt ctagccaagc atggggatct gcttttgtat
    16621 gttcttcacc gctagttaaa taaagaggtt ctacattttt gctaactgca aaataatctt
    16681 ttccttcttc ttttttagcc gtttccatca agttatcgaa ccagctgtta cctgtttcaa
    16741 gattcaaacc gttgtataaa ataacatcag catcgcttgc ctttttgatg tcttctggta
    16801 gtacttcata ttcatgcgga tctgttccga ctggtacgat actgtggata tctatttgat
    16861 ctgttccgac ctcttttgcc atgtccgcca agatcgaatt ggtagccacg actgacaact
    16921 tttctttact tccttcatcc gcttggtcag aagcctgctt tccgcaaccg gctaatacca
    16981 agaaactaac tgccaagatt aaaaagagtg atttttttgt tttcatctat ctgcttcctt
    17041 tctgaaataa aattcctttt gttggagaaa ataaaaaagc aaggataaaa aatgcggtac
    17101 atgctaacac gatcgtagct cctgatggcc aattgtaact ataactgaaa aacacaccga
    17161 caactgcact tagcataccg attcctgcag ctgtaatgat cattttcagt aaatggttcg
    17221 ttagcaagta agccgtggcg gctggtgtga tcaacatggc aatcaccaag attgttccta
    17281 ctgtctgtaa actgactaca gccactaatg tcaaaaagaa catcaaaaga taatggatca
    17341 gcgatgtgtt cagcccataa gcttttgcca ttgtcggatc gaatgatgtg atcttcaatt
    17401 ctttataaaa gaaaaagaca aatatcaaga cgattccact aacgagtgct gtcagcaaca
    17461 aatcgctttc cctaacagct aagacattcc caaataaaat atgatacaaa tctgtcgcac
    17521 tctgggcata tgaaatcaag ataatcccta atgcaaaaaa ggagctaaag acaattccaa
    17581 tcgctgtatc atttttcaat cggctccgct gagtcacgaa accaatcact gctgctgtta
    17641 gcatcccaaa agcagtggct ccaaagatgt aactaaaacc aaacatataa gaaatggcta
    17701 cacctggaag aactgcgtga gagatagcgt ctcccatcaa agacatccct cgtaaaataa
    17761 taaaagaacc gatgactcca gaagcaagtc ctacaagcat ggaagtgacc aaagcgtatt
    17821 gcaggaattg ataattcatt aatccatcaa taaaggattg gatcattttt tctctcctcc
    17881 ttttactaaa tgtctgatga tctcaccata ggccaattga aggttcttcg atgtaaagac
    17941 ttcttgtact ggaccggaag cgatcagctg tttatttaaa agaataacct catcgaaata
    18001 ttcttctact ttatgaagat catgatggac gatcaaaatc gtttttcctg aattcttcaa
    18061 ttcctgcaag atgtcaaaga tctttcgttc acttaacgca tcaatcccta cgaatggttc
    18121 atctaaaaag atccattctg ctccttgggc tagagctctt gcaataaaaa ctctctgaag
    18181 ctgtccaccc gatagttcgc tgatctgtct tttggcatat tcttccatcc ctactttttt
    18241 caaagcttgt cttgcacgtt ctttttcttt tttcccaggt ctttgtccaa ttcgtaaaga
    18301 tggatatgtt cctaaaagta caacgcctaa tacatcaatt ggaaaggaaa gatccaattc
    18361 acttcgctgt tccacgtagg ctattttttt acggactgag tttaccggct gtccagaaaa
    18421 ggtgacttta ccagtagtag ctggtatcaa ctctagcaaa gatttcataa atgttgattt
    18481 tcctgcaccg tttggtccga taataccggt aattttttgt ggatttatcg tcaagttgat
    18541 agttttcaaa acagttttcc cttgataagc ggctgaaagg tcttgaatta caatggccgt
    18601 acgttccaaa tcttacactc ctttttaggt ttacctaact ttttatttaa atatagttta
    18661 actaaaatgg aaagcatttt caagtagaat gatgaaaaag tttttttcga attttcctcg
    18721 cattttatga aaattttctg tagaatcaca tcaagagttc aataaggcta acctaataat
    18781 gaaaaacatg tgttgaaacg tttgattatt ttttcttgtt gactcaacaa cgaaaggagt
    18841 ttttgtatgg aagaagaaaa gttaactcga agtcttagtg cccgtcatat ccaaatgatc
    18901 gcactaggtg gaacgatcgg tgtcggtctg tttatgggtg cctcctcaac catacgttgg
    18961 acagggccct cagtgatggg agcctatgcg attgcaggat tctttttgta tttgattatg
    19021 cgtgctttag gcgaaatgct ttacgttgat ccttcgactg gttcttttgc aaattacgcg
    19081 agcgagtaca tccatcctgt ggctggctac ttgaccgctt ggagtaatat ttttcaatat
    19141 attgtcgtag gaataagtga agtgattgct gtaggatctt atatgaatta ttggtggcct
    19201 gatttgccgg caattattcc cggaatcatt gtcgtcttgt ttcttatgct ggcaaatctt
    19261 atttctgtca aggcgtttgg cgaattagaa ttttggtttt cgatcatcaa agtcataacg
    19321 atcattttga tgatcattgc aggtcttggg gtaatcttat ttggatttgg taatcatggt
    19381 caggcagtag ggatttcaaa tctttggaaa aatggtggat tcttcactgg aggcgtcaaa
    19441 ggatttttct ttgcattatc gatcgttgtt gcttcctatc aaggaatcga actgatcgga
    19501 atgactgctg gtgaagcaga gaatcctaaa aagacgatca ttgaagctgt ccagtccact
    19561 atcggtcgaa tcttgatttt ttatattggt gctattttta tcattgtgag tatctatccg
    19621 tggaatcaat tgagtgagat tggttcacct tttgtacaaa ctttttctaa agtcggcatt
    19681 acatttgctg caggactgat taattttgtc gtgattacag cggcattatc aggttgcaat
    19741 tcagggattt tcagcgcaag tcgtatggtc tatacacttg ccataaatgg aaagatgtct
    19801 aaaaaatttc tgaaactatc tcgtcacggt gcgccttttt atcctgtcgt agcaatctca
    19861 ggtggaatct taattggtct gatcttaaat tatcttctac caatgttcat caaccagtca
    19921 gaaaatattt tcgttttcgt atacagctca agtattttgc ctggtatggt tccttgggta
    19981 gtgattttga tcagcgagat tcgcttcaaa aagaagcacg cagataaaat gcatgatcat
    20041 ccttttaaaa tgccactatc tccttatacg aattatgtga cacttgcttt tttagctatc
    20101 gtattgattt tcatgtttat caatcctgaa acgagaatct cattgattat tggactcgtt
    20161 ttcttgcttt acatgacgat ccattacttt gtacgcgaaa aaagaaccgg aaaataaaaa
    20221 aagaacgaag cttcacctta gtggaagctt cgttcttttt tattgctttt atcaattcaa
    20281 ttttagcgtc tcaccgggag tcaggaaata attctccatg ctcatcccat tcagcgacaa
    20341 gatcgtttct actggtacgc cagttcgttc ggctatttga tttggacctt ctccttccaa
    20401 aactacggtt gtcgcagcct gtgaagtcgc gctgttggta ttagtttcag aggaatctgt
    20461 tgtgtcttgt gttgttcgag cttgcatttc agaagcttct accgactctg ctgtctcaga
    20521 atcagaagat gttgtgtctt gtgtgcttgt tgactctgct tgaagagagg cgctagtctg
    20581 caaataaata tcggaaatct tagcgacatg attcgtataa ttagccgata attgattgat
    20641 caaagtatct aagtctacac cgtctttatt ttctgctgcc tgatacgctg cacgtaattt
    20701 tctgatccct tcgtcggaga ttgcttgaag ctcacgtgct ttttggttag caactgctga
    20761 taaaccagct attccctctt gatttccctg aatttctgtc tggtattctt caatcaatgc
    20821 aggcgtcttt tcttcgatct ttttcgaata atcatttacg attgcttgaa gttgctctgg
    20881 caaaccattt gctgtttttt cttcagaagt ttgtgtctct tttgttttag ttgatgactc
    20941 acttgtttgt ttcgtctctg ttttctttgt cgttgtttcc gttatggtcg tacttttcgc
    21001 ttctgacgaa ttttttccat gaaaatatag acccgcaact cctcctgcta tgatcaaaaa
    21061 cacggcaact gctgccccaa tcatgatcat ccgcttacga gctgcctgtt tttgctgtct
    21121 tcttcgggtc atccgaacat tttctcccca ctcattttca tcatacattt ttttcaccac
    21181 caatttttat gtataatagc gttatcaaac gcttcatatt cttattgtag caaataattg
    21241 tcgctgtgca catttttatc tttgttattt ataagaaaat ttcaccagtt gagatgattc
    21301 caagattgtc cgtcgatttc tccttgcagc tgcattgttc gttcgtctat ctcagccatc
    21361 agtttcttta tttcatataa gagcgcacgg tcttgatatt ctgatttttc gcttaattca
    21421 tctaattgtt ggatcagcca atcatttgtc ataatgaagt tcctttcatt tcttgcagtg
    21481 taaaggtcaa gtcttcgatc tgctcttttg cctcagtcag tgcttctacg atatatgctt
    21541 gtttttcaga ttcttcttcc tgcattttct gattttcctg tactctttga ataaaccagt
    21601 caatccgctg agtcagcgat ggtttgcctt ccaatgagag atactgctga taagcaatta
    21661 gcttctccca acgtttttcc tgggataaat acataaattg tgctaaaacg aatggcggaa
    21721 gataggtcat atggaattta tttgcgattc gctgatacgg cctcaaaaat tcggaaatcg
    21781 tatattcttc ttttcctcca gcttggtatt ctttttccgc cacacctgtt gtcaatacga
    21841 ttcctagttc tttccctttt aatacatgtg ctgctcgttc caagacatta tcttgccaaa
    21901 tttttaaatg agcaggagaa ctataccagt aaaacggaaa ctggaaaatg atccgatcat
    21961 gctgggccaa gagctgtagt tcagtctcta tatctatttt tccgtctgga taacttgatt
    22021 ccaaatgatg ccaagtagct ggtaaaccag cagaagcttc tttgaaaaat tgctgattta
    22081 aagattcttc gatcgttgga tggctaatga tgataagtgt acgcatccct tttcctcctt
    22141 tttagtcttc gctttacagt gtagcataac cgccacaagt ttcttttgcc gatgcttgtc
    22201 attttaacca gactaatcag gttgtccaag aaactgcggt ttgtttttcc aactgttttt
    22261 tcaaggtatc caaagcaata ttcttaaaat tttcgttttt catacgttta cgatagttcg
    22321 tagccgtttc tccggtactc tttttgaaca ttcggctaaa tacgactaga ctattgaacc
    22381 ctacttgata ggcaatactt tccacggatt catccgtcag agaaagaaga aacgcccctc
    22441 gattgatccg taccccgatc aaatattcct tgatactggt acccatatga tttttgaata
    22501 ctcgcgacaa atggctccga ttgaccgcta aatgctgggc gatttcctca acagacaatt
    22561 tttcagcata atgtgcctcg atgtatttga ctgtttcaac agctaaagag gaatgaacct
    22621 tttgtacacc accgctgacc tttccgccat cttttaaaag gactgataaa aaacggtagg
    22681 ttaatgctgt taactgtaat tcattttcca aggtttcttc cgtatctttc atgcattcta
    22741 aaataatgtc gatatatttt tgtatgtcag aagagaccat cgtgtattgg tcttctctga
    22801 aaagcgactg ccgaatgatt tcttctgcag cactcccacc aaatgaaagc cagcaataca
    22861 tccacggatc ttctccatct gataagtaaa acgtcgagtc tcctggacga atcaagaaca
    22921 gatcaccttt tttcaattgg aattgttgat ttttcacatg ataagatcct ttgccttcca
    22981 tcacgacatg caggatgtaa tcattgcgaa tagcaggacc aaaagaatga aacggcatcg
    23041 ttttagagaa accacaaaaa tcaaaataca attctttttc tacttgttga tgttcctttt
    23101 ttaaaaaaat cgattgctcc acgtctatcc ctccttactc ctttagtata atgcaacaaa
    23161 aagttaaata aaagtcacag aaatatatta aaaaggaagc gatttcttgt taagtttaag
    23221 tcattaaaag atctttgtta ttcgttgtaa ccggtttact tagtattata tattgaacag
    23281 gagaggaata cagattatga aaagcaaaat gatccaacgt gtgtcctacg ctttaggcgc
    23341 ttttggacac gacacattct atgttacatt aagcacttat tttatggtat tcgtgacaag
    23401 ccagatgttc gcaggaacag ataaggcaac gaatgcaaaa atgattgcta cagttacaag
    23461 cttagttgta ggtatccgct tagttgaaat tatttttgat cctttgattg gtggcatcat
    23521 cgataatact cgtacaaaat acggtaaatt caaaccatgg ttagttatcg gcggcgttgt
    23581 tagttccgta ctcttggtag caatctttac caatttcttt ggcttagcta ctcataatac
    23641 gactttattt atcatattat ttgcaatcgt tttcatctta ttggatagtt tttattcatt
    23701 taaggatatc gctttttggt caatgattcc agctttgagt acggaaacaa aggaacgtga
    23761 aaaatttgct acgtttgctc gtttcggctc ttctctaggt gcaaatggta ctactctagt
    23821 cgttgtccct atcgttactt tctttaccta tcttgttact cataaacata ttgaaggcgc
    23881 ttcaggctgg ttctggttcg gtgtgattgc tgctgttatt tcaggcggta ctgcgattat
    23941 cactgccttg ggcacaaaag aagtcgatac tgcgatccgt cagcaaaatg aaaaagctgg
    24001 gatcaaagat gtgttcaaag cagttgctca aaatgatcag ttgatgtggc ttgcccttgc
    24061 ttatatcgct tatgcgattg ccaatgtagc aacaactgcc gtattattct acttcttcaa
    24121 atatgtactt ggccaaccaa ctgaattttg gatcgttggt ctagttgctg ccttacttgg
    24181 tttagtagct gttccactat tcccaatgct tacacgcgtc atctctcgtc gctacgttta
    24241 ccttggaggg atcgcaatga tgatttcttc ttactttttc tttacgatcg caggttcaag
    24301 tttgccaatc gttatcatcg ggctagtatt gttctacttc cctcaacaat tgattttctt
    24361 atccgctttg atgacgatca cagactctgt tgaatatggt caatggaaaa atggtatccg
    24421 taacgaagct gttactctat caattcgccc tttactagat aaaatcgctg gtgctttgtc
    24481 taacgggatc gtagggtttg tcgctatcgc ttctgggatg actggtaatg ctactgctgc
    24541 tgatatcact gctcatggcg taacgacttt caaagcttat gcattctatg ctcctgctgc
    24601 tttgatgatt ttctccgcta ttatttttgc ttggaagatt acattgacag agaaaaaaca
    24661 tgcacaaatc gttgaggaat tggaaaacaa attggctcca gctgatacaa tggaagatga
    24721 acttcacgat gcagtagttc cttcccatta agaaaggatc ttgaaaatga agattacaga
    24781 aaaagatttt ggccaaggtt atcacttgat aaccttggag aacaaaaaca agctagcact
    24841 ttctatctct gatttaggtg ctagaatcgt cagtttaaaa tcaaatgatc gagaattagt
    24901 tttgggattt gacaccgctg aagaatatat cgaaaaagat ccgtatatcg gtgcttctat
    24961 cggtcgcacc gcagggagaa tcgaaaatgg ccgcttttct ctcaatggaa aaacttacca
    25021 attagccaca gatccaaaaa ctggacataa tctgcatggt ggtgctcctg gatttgaatt
    25081 aaaaaaatgg tcttatgtca ttttgaatgg cgaaaatgaa gcttctgtca tttttactac
    25141 tacaagtccc gacggagaac atggttttcc aggtacaatg gatgtggaaa tccgctatac
    25201 tttgacaaaa gataatatct ggcgagtgac ctctcgcggc accagtgacc aagatacttt
    25261 attcaatccg acaaatcatg tttacttcaa cttgacggga gatgcttctc agtcaatcga
    25321 tcaacatgag ctttggctca atagcgaagc ttatgctcct ttgcgaacag atagtattcc
    25381 tataggggta aaagaaaatg ctgcaggaag tgctttcgat tttcaaatac ctaaaaaatt
    25441 agcgtccgtt tttgctagtg atttggatca aaaaaatctt gttgacggaa tcgatcatcc
    25501 atttttctta aaagaaaccg gcttgggtaa agaagccgct cgcttgacta gccccgataa
    25561 aagaatccaa gtaggtattg ctacagatgc ttctagtgtg gttattttca ctgcaaattt
    25621 cggcacagaa acaccagaaa tgcgcaaccg aaaactggca catcacggtg gcatcacatt
    25681 tgagactcaa acagctccag gtgcagagcg cttctcagca tttggttcga ttcatttaaa
    25741 agctggatca gtatttgaaa cagtgacaga attcaaaatc aaaacaagaa aggaataact
    25801 catcatggaa atcaaaagaa gcttaaacac aaaatttgcc gaaacattcg gacctaaaaa
    25861 aactacacaa tacttctcac caggacgcat caatttgatt ggtgaacaca cagattacaa
    25921 tggaggccat gtcttccccg cttccatcac ttatggaacg caaggtgtag cagcacctcg
    25981 agacgataat aaaatattag tttattccac aaactttgac gatatcggtg tcattagttt
    26041 tactttagat gagctagaat atgataaaaa agcaaactgg gcaaattacg taaaaggaat
    26101 ggtccttaaa ttaaaagaag ctggacactc aatcgatcat gggtttgagt tgttagtgga
    26161 aggaacaatc cctaatggtg caggactttc cagtagcgct tcattagaat tattagtagg
    26221 tgttgtatta gaagatctgt ttgatttaaa aacagatcgc ttggaactag tccaaacagg
    26281 taaaaaagta gaaaatgaat tcatcggtgt caattcaggt atcatggatc agtttgccat
    26341 cggcttcggt gagctagatc atgcaatctt attagataca aatacgctga aatacgaaat
    26401 ggttccagtg aaactagatg gttatgcaat cgtcatcatg aatacgaata agcggcggga
    26461 attggcagat tcaaaataca atgaacgccg tagcgaatgc gaagaagcct tggcacgttt
    26521 gcaaaaaact ttgcctatct cttctttagg agacttagat gaagaagaat ttgaatcaac
    26581 tattgaccaa atcggcgatg atacattgat acgcagagct cgccatgccg tttctgaaaa
    26641 tcagcggact ttgaaggcaa aagctgaatt agaagctggg aacttagaag catttggtca
    26701 actgttaaat gattctcacc actctcttcg ctatgattat gaagtaactg ggatcgaatt
    26761 agatacacta gtagatgctg ctcaaaaaca agaaggtgtc ttaggtgcac gaatgacagg
    26821 agctggattt ggtggctgtg cgattgctct tgtcaaagaa gaaaatatac ctgccttcaa
    26881 aaacaatgtc tatgatgaat acatgaaagt tgtcggctat gctccggaat tttatgttgc
    26941 acacattgga aatgggacaa caaagatcga tccagaagct gttcatgttt gataaaaaaa
    27001 tcaacgatct cagctaaaaa caaatagata aaaataacct ctcagcaatt tccaaatgtg
    27061 agccttttgc tcgtatttga aaattgctgt tttttattga cacggaaaga gaacgtgtgt
    27121 tctaattgtg ttgaagaggt gattgttatg tgggcgaatc aaaatccggt atttgtttat
    27181 gaaaaagaac cttcaagaga tattttatgt attgactgca aatcttttta cgcctccgta
    27241 gagtgcgttg aaaaggatct tgatcccctt acaactaaat tagtagtcat gtcctatccg
    27301 tccgattcga cagaaacacg gggtagcgga ttaatcttgg cttccagtcc tgcagcaaaa
    27361 aaagcttacg gcatcaccaa tatcagccgt gccagagatc ttcccttccc ctatccaagt
    27421 gatttatata ttgttgcccc aaggatggcc tattatatgg aaaagaacag agaaatcaat
    27481 tctatctata aaaaatatgc agacgaagcg aaccatcatg tctattccgt cgatgaaagc
    27541 ttccttgatg taacaaatag catggcactc tttggtaaaa caaatgcgta cgacatggct
    27601 aaactgatcc ataaagatgt gtttgagcag accggaatct acacgactat aggaattggc
    27661 gataacccgc tacttgctaa actagcctta gacaatggct caaaagacgg aaagcaaatg
    27721 tttgctgaat ggcgttatga agatgtacca gaaaagttat ggaaaatctc agatatcact
    27781 gacttttggg gaatcggcaa acgaacagca aaacgtctga aacaaatggg tatacattct
    27841 gtttatgatt tagctcacgc ggattattat cacttgaaaa gcaagctagg ggtcatcggt
    27901 actcagcttt atgcccatgc ttggggcatc gatcgaagtt ttttgggaca agaatataca
    27961 ccaaaatcaa aaagtatcgg aaatagtcaa atcctagata gagattatac aagacgagaa
    28021 gaaatcgaga tcgttatcaa agaaatggcc gaccaagtcg gcactcgctt gagacgatct
    28081 gcagcaaaaa cacaagtagt cagcttatgg gtaggatact ctttaggcta tcttgatcca
    28141 aatggcaaga ccgggtttca ccagcaaatg aaaatcgaag cgacaaatgc tagcagagat
    28201 ctagcagatg ctttattgac cattttccac cagcattacc actaccagga tattcggcgt
    28261 gtaggcgtga actgttccga cctcgtatac gcgacaggtt tacagcttaa cctttttgaa
    28321 gatcctgaaa agcagataaa tgaagcgaaa gttgattttg ttgtcgatac gattagaaaa
    28381 aaatacggct ttcaatcaat catccatgcg catagtctct tgcaaggagg acgcgccatc
    28441 gcacgtagtt ctctcgttgg cgggcatgca ggtggtatga gtggattaga aggtggaaaa
    28501 gatgagacgg acagcaaaaa aattcattga ctataacgag tatcgagatc ggccatttgg
    28561 attaaaatgg gggactgctt ttgcgatgga agaactagtc aacggtatca aaaaaaatga
    28621 agaagaagca cgcaaacagc ctcttcctaa acaacaaatg acattgcaag aaatcgatag
    28681 tatactgaca gaagcctttt tatctcgtaa agcagtaatc attcagctca atttaaaaga
    28741 tgaatttgga agactcttgg atgaggtcga agggcacttc attggtgaag cctatgaaga
    28801 ttactttatg attgaccatc agccgatatt gtgggaagac gtacggtata ttgagttaaa
    28861 ggaaacaaaa aaatggtttg tgacatgaga ggttgtgaca gaagtgatca acttcaaaaa
    28921 ataagaaagg atttttcgaa aattgcttct taaatttttg tggaattttg gcttattttt
    28981 cgaagaagca acttctggaa caacgtatat tagatatcag aggttgtgaa aaagtcgctt
    29041 agttttgagt aacaagccaa ggcgctgatt tcacaacctc tgtctatcta tccatctatt
    29101 cggtaagatt catgcatgac gtcttcttta tttagtatgt agagcccttt ttcttctgac
    29161 cattcaacat aaatcagctg ttttacatct gcatagcctt ctttcttcaa gttctccata
    29221 agccatgctt cagtcaattg attggactga agcactttat gctgaatttg cccttcatct
    29281 acaagcagga tcgataaagc tttgtcttca tctgattttt tcaatacact catttgacca
    29341 gcattctcta ggataacatg tttggcattt tcaagagaaa agcattcttg ctgtcttaag
    29401 attgctcgca actgctccat ctcgatatgg ttatttgtca actctgtcat gttgatgacg
    29461 ccgtctttga ttagaacaga tggctcgcct ttgacccaac gattgacctt ttcatttttt
    29521 tggacgatac gttcgatacc ataaatcatt actgcccaaa gcgcaatggc aaataggaca
    29581 tgccctacat ggacattgtc atcataggtt gattcttcta agatcccacc taaaacaagc
    29641 gtatagacta aatcaaacgg tgtgatttct gacatggatt tcttcccaag caaacgtacc
    29701 actaataata aaccgatcaa cccgaacacc agtttcattg taattgaaaa aatcattttt
    29761 tctcccccta actatctgtt aattaaatta tatatgcttt ctcacgaaca cgcatggaat
    29821 atacaaggaa gaaatgtatc cttttactaa aaaaaggact gtgacactgt agtgaccccc
    29881 aaaagttaga cttttttgga gtggagaaat ccactccttt tttttatgct ctaaaatgat
    29941 atttttacac aaccgttacg gttgttttac gaaattggac g
//
LOCUS       DAJSBM010000026        28197 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJSBM010000026 DAJSBM010000000
VERSION     DAJSBM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.026"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            160..1302
                     /locus_tag="ONT67_001422"
     CDS             160..1302
                     /locus_tag="ONT67_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR2355174.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            1318..2529
                     /gene="thiI"
                     /locus_tag="ONT67_001423"
     CDS             1318..2529
                     /gene="thiI"
                     /locus_tag="ONT67_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR2355175.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            3102..3749
                     /locus_tag="ONT67_001424"
     CDS             3102..3749
                     /locus_tag="ONT67_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCR2355176.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    3878..4094
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4142..6787
                     /locus_tag="ONT67_001425"
     CDS             4142..6787
                     /locus_tag="ONT67_001425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR2355177.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            7097..8410
                     /locus_tag="ONT67_001426"
     CDS             7097..8410
                     /locus_tag="ONT67_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR2355178.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            8400..9053
                     /locus_tag="ONT67_001427"
     CDS             8400..9053
                     /locus_tag="ONT67_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2355179.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            9108..9659
                     /gene="radC"
                     /locus_tag="ONT67_001428"
                     /pseudo
     CDS             9108..9659
                     /gene="radC"
                     /locus_tag="ONT67_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            9790..10875
                     /locus_tag="ONT67_001429"
     CDS             9790..10875
                     /locus_tag="ONT67_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2355180.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            10872..12764
                     /locus_tag="ONT67_001430"
     CDS             10872..12764
                     /locus_tag="ONT67_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2355181.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            12771..13148
                     /locus_tag="ONT67_001431"
     CDS             12771..13148
                     /locus_tag="ONT67_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2355182.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            13302..14084
                     /locus_tag="ONT67_001432"
     CDS             13302..14084
                     /locus_tag="ONT67_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2355183.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT67_001433"
     CDS             complement(14210..14941)
                     /gene="erm(A)"
                     /locus_tag="ONT67_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2355184.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14999..15058)
                     /locus_tag="ONT67_001434"
     CDS             complement(14999..15058)
                     /locus_tag="ONT67_001434"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2355185.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            15450..16112
                     /locus_tag="ONT67_001435"
     CDS             15450..16112
                     /locus_tag="ONT67_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2355186.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            16347..16490
                     /locus_tag="ONT67_001436"
                     /pseudo
     CDS             16347..16490
                     /locus_tag="ONT67_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            16640..16840
                     /locus_tag="ONT67_001437"
     CDS             16640..16840
                     /locus_tag="ONT67_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2355187.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(17094..18773)
                     /locus_tag="ONT67_001438"
     CDS             complement(17094..18773)
                     /locus_tag="ONT67_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2355188.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(18775..18987)
                     /locus_tag="ONT67_001439"
     CDS             complement(18775..18987)
                     /locus_tag="ONT67_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2355189.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            19380..21110
                     /gene="efrA"
                     /locus_tag="ONT67_001440"
     CDS             19380..21110
                     /gene="efrA"
                     /locus_tag="ONT67_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2355190.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            21107..22867
                     /gene="efrB"
                     /locus_tag="ONT67_001441"
     CDS             21107..22867
                     /gene="efrB"
                     /locus_tag="ONT67_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2355191.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            22962..23159
                     /locus_tag="ONT67_001442"
     CDS             22962..23159
                     /locus_tag="ONT67_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355192.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            23312..23797
                     /locus_tag="ONT67_001443"
     CDS             23312..23797
                     /locus_tag="ONT67_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR2355193.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT67_001444"
     CDS             complement(23901..24494)
                     /gene="clpP"
                     /locus_tag="ONT67_001444"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR2355194.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            24674..25201
                     /locus_tag="ONT67_001445"
     CDS             24674..25201
                     /locus_tag="ONT67_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355195.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            25292..26029
                     /locus_tag="ONT67_001446"
     CDS             25292..26029
                     /locus_tag="ONT67_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2355196.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            26033..26950
                     /gene="miaA"
                     /locus_tag="ONT67_001447"
     CDS             26033..26950
                     /gene="miaA"
                     /locus_tag="ONT67_001447"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR2355197.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            26952..28181
                     /gene="hflX"
                     /locus_tag="ONT67_001448"
     CDS             26952..28181
                     /gene="hflX"
                     /locus_tag="ONT67_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR2355198.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         9420 a         4609 c         5934 g         8234 t
ORIGIN      
        1 aaaagttagt aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt
       61 atatttgaaa attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc
      121 gttataatag aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt
      181 gcaacaaccc caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt
      241 attgggaatc catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa
      301 gcaagaaagc agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca
      361 ggcggaacag aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat
      421 gggaatcata tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa
      481 ttaaaagaat taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt
      541 gtcgacgaat tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg
      601 aataatgaag taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt
      661 cctaaaattc atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa
      721 tggttgacag aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg
      781 ggaactggtt tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga
      841 gggcaagaaa gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt
      901 aaagctttac gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta
      961 aaagtagctc taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga
     1021 actgactatg caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc
     1081 gtccatgcct tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt
     1141 aaacatatgg caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct
     1201 gctgttcgaa tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt
     1261 attttcaagc agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg
     1321 aaatacagtg aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa
     1381 agctttatca tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc
     1441 aaaatccatg ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta
     1501 gttattccaa aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta
     1561 gaaaaagatg tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact
     1621 gggaaagaaa catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt
     1681 aatgaattga atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt
     1741 caaatgcgtc aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta
     1801 tcgtatgaaa cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg
     1861 cttatgcttt ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga
     1921 gtggaaattg aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa
     1981 aaagcaaaag atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa
     2041 gtgccattta ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg
     2101 acaattactc gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt
     2161 ttagtcattt tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg
     2221 gtggctatca atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa
     2281 cttgaaatca ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt
     2341 gaagattgct gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag
     2401 gtccatcaat atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc
     2461 ttgaagatgg aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa
     2521 ttgctataag aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa
     2581 aaatagctcc atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac
     2641 catttatcaa gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt
     2701 atttagagat caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag
     2761 cgaggttgtg acagaagtgg gcagcttcaa gaaataagaa ggaatttacg aaaattgctt
     2821 ttcaaatttt tgtgaaattt cggcttattt ttcgaagaag ttacttctgg aacaccgtgt
     2881 atcagaggtt gcgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2941 ttcctcatat tttacgtttg gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt
     3001 tttatttgtc tcacaccctt ttccttccta cttgtaacct tgctcccgtg ttagaaacat
     3061 gttacactaa atatgtgaaa ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata
     3121 cactgccaaa agcaactgcg aaaagattac ctttatattt acgctacttg aagatgctgg
     3181 atgactcagg tataagtaga atcaaatcaa atgagttcag cgaaatcacc caaattcctt
     3241 ctgcaacgat ccgcagagac ttctcgcaat taggcgagct aggcagaagc ggatatggct
     3301 atgatgtccc ttttcttatc gatgtattca ataatatcct taatactaaa gaagaaaaac
     3361 gaatcgcatt agtaggatat ggaaatcttg gcaaagcatt gaaacacaac aattttagaa
     3421 gaaatgaaaa tctaaatatc gtgtgcgtct ttgataatga tccagcactg atcaatcgag
     3481 taattgatgg agaaatgatc tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa
     3541 atgtgacagt agcaatctct acggtaccaa gtaaatattc acaatctgct attgatgaaa
     3601 ttgtcaaagg aaatgtaacc gctatactta attttgcgcc tgacagagta actgtcccag
     3661 cttatgtcaa tgttcaatac attgatttga caaccgagtt gcagacgctt atttactttg
     3721 atgagaatta ctcagaagtt ttttcttagg aaaatttgag taggcttatt gatcttgcga
     3781 gaactcgtcg taattctttc aaaaattttt tagtactaaa tgaagtggtt ttttcacgac
     3841 cacttgttga ctaaaaaaaa aaaccattgt agaatgaagc ataacgctga acaagaggag
     3901 taactggcac attcattctt agagagagaa ctatctgctg aaaggttctc aatgacaacc
     3961 agtgaaggta gcttgggagt ttctgtcttg aatggagtag agatagacga gtcgtgatcg
     4021 ttaactcact tgagaggcaa taatgattga ttattgcaat ttaggtggta ccgcgtaaat
     4081 catacgtcct agaatacgaa agtattttag gacgtttttt agtttaaagg aggaaaacag
     4141 catgacggaa gaaaataacc tatcaacaaa atataatccg caagaagttg aagctggacg
     4201 ttatcagcat tggttagaac aagatttgtt taaaccgagc ggagataaaa aagcaaaacc
     4261 ttattcaatc gtaatcccgc ccccaaatgt tactggaaaa cttcatttag ggcatgcttg
     4321 ggatacgacc ttgcaagata tgctgattcg tcaaaagaga atgcaaggat atgatacttt
     4381 atggctgcct ggtatggacc atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct
     4441 tagagagcaa gggatttctc gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg
     4501 ggaatggaaa gaagagtatg cgagccacat ccgcgaacag tgggctaaaa tgggcttatc
     4561 tttagattac agtagggaac gttttacatt agatgatgga ttatctgaag cagtacgtaa
     4621 agtttttgtt accttatacg aaaaaggttt gatttatcgt ggtgaatata tcatcaactg
     4681 ggatcctcaa gcacgaacag ctctatctga tatcgaagtt attcataaag atatcgaagg
     4741 cgccttttat catatgagct atgaactagc agatggatca ggcgtagtag aaattgcgac
     4801 aacccgtcca gaaacgatgt tgggagatac ggcgattgct gttcatccag aagacgagcg
     4861 ttatcaagcg ctcatcggca aaaaagtgat tctaccttta gtcaataaac aaatcccaat
     4921 cattgcagat gaatatgtag atatggaatt cgggacaggt gtagtaaaaa tcactccagc
     4981 acatgatcca aacgactttg aggtaggtaa tcgtcatgat cttccacgcg taaatgttat
     5041 ggatgatgat ggtacgatga atgaactagc aggaaaatat gctggaatgg aacgttttgc
     5101 tgctcgtaaa gcaatcgtgc aagatctgaa agaaatcggt cgattgatta aaatcgatac
     5161 gatgatccac agtgtgggtc attccgagcg gacaggtgtc gttgttgaac ctcggctgtc
     5221 tactcaatgg tttgtaaaaa tggcgccgtt agcgaaaaaa gccattgata accaagaaac
     5281 agatcaagca gtggaattct tccctccaag attcaatcaa acgtttttac gttggatgga
     5341 aaatgtccat gattgggtaa tctcacgaca attatggtgg ggacatcaaa ttcctgcatg
     5401 gtatcacaaa gaaacaggtg aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa
     5461 ctgggtacaa gatcctgatg ttttagacac ttggtttagt tcagctttgt ggcctttctc
     5521 aacaatgggc tggccagata cggaaagcga agattataag cgatacttcc ctactagcac
     5581 tttagtaacc ggttatgaca tcattttctt ctgggtaagc agaatgattt tccaaagctt
     5641 ggaatttact ggcgaacgtc catttgagaa tgtcttgatt catggattga ttcgtgacga
     5701 acaagggcgg aagatgagta aatcattagg taatggaatc gatccgatgg aagtaatcga
     5761 aaagtatgga gcagatgctc tccgctggtt cttatcgaat ggttctgcac caggacaaga
     5821 tgtccgcttc agctatgaaa aaatggatgc ttcttggaac tttatcaaca aaatctggaa
     5881 tgcttcacgt ttcgttatca tgaatatcga aggaatgacc gtagaagaga tcgatttatc
     5941 tggagaaaaa tcagtggctg accggtggat ccttactcgt ttgaatgaaa cgatcgaacg
     6001 agtaactgag ctatttgatc gttttgaatt tggtgaagca ggtcgacaac tgtataactt
     6061 tatctgggat gatttttgtg actggtacat cgaaatgagt aaagaagtac tttatggaga
     6121 tgatgctgtt tcaaaacaaa ccacccgtag catcttagtc catacacttg atcagatcct
     6181 acgtttattg catccaatca tgccattcgt tacagaagaa atatgggaac atattcctca
     6241 tcagggaaat tctctagtag tggcagaata tcctgttgta cgtccagagt ttgatgatga
     6301 aacagcttct aaagggatgg aagtattgaa agaattgatt cgagccgttc gaaatattcg
     6361 ttcagaagtg aatacaccat tgtctaaacc aattacgttg ctaattaaaa caaacgatgc
     6421 taaaatcgat caatttttag ttgaaaatac aagctatatc gaacgcttct gtaatcccga
     6481 agagttgacg atatcaagtg agatcactgc gccagatcta gcgatgtcag ctgttctgac
     6541 aggtgcagaa atcttcttgc cgttagcagg attgatcaat attgaagaag aaatcaaacg
     6601 attagaaaaa gaactggaaa agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc
     6661 taatgaacgt tttgtttcta acgcgccgga agaagtagta gaggcagagc gtgcaaaaga
     6721 aaaagattat ttagagaagc aaacggctgt caaagaacga atcgaaagtt tgcgtactat
     6781 tcaataagaa tagaaaaaga gagtgtgaaa tcagctagct attactaaaa gctagctgat
     6841 tgaacatcgt tacaggaaag agtttgagcc tcactcttag cttcgattca taaggagatg
     6901 tgacataaat tttgtcgcat ctctttttta gtctactctt attatgtagg agaaggaggg
     6961 aggtggatca tgtatcaaga gtcaataaag tagttgataa aatcgttcct ctctctgttt
     7021 cttttctctt tgtcatactt ttatttttct tatgtgttct atttgttata ataaaagaga
     7081 aatgggggaa ttgttgatga cgattgaaga agcaatagaa tggatccata gtcgattacc
     7141 atttggttca cgtcctggcc ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc
     7201 agagagaaaa gtaaaaacga tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc
     7261 ttatttgaga agcctattag aagaggctgg gctgacagta ggtacattta cttcacctta
     7321 tatcgaaacg ttcaatgaac ggatcgcaat caatggcaaa agcattccag acaatgattt
     7381 gatccagctt gtaaaaaaat atcagccact gatcaaacaa cttgatcaat ttgaagcagt
     7441 agctggtatt actgaattcg agacattaac agcaatggca tttgattatt ttttgatgaa
     7501 gaaagtggac gtagcgatta tcgaagttgg cttaggcggc ttattggatt ctaccaatgt
     7561 agctgtacca atgctgacgg gaataacaac gattggactt gatcataccg aaatacttgg
     7621 agatacaatt gaagaaatcg ctgcacaaaa agctggaatc atcaaacaag gcgttccagt
     7681 agtaacagga aatattgccc cagaagcttt tactgtcatc caacaaacag ctaaagcaaa
     7741 aaatgcaccg ctttatgtat acggggaaaa ttataaaata aactatctgc atcctgatga
     7801 gatgtgggga gaagtatttg aattttttaa tgaaatagga aagatccctc aattaaccac
     7861 tccgctttta gggcaacatc aagctgaaaa tgcaggagta gccatcgaat tgtattttgt
     7921 ttactgtcaa ttggaaaagc tgcctttccg agaaaaagat gttttgaagg gattgaagaa
     7981 atctcgatgg ccaggaagaa tggagcgttt gagtaacgag ccattagttg ttttagatgg
     8041 tgcccataat acgcatgcaa tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg
     8101 atatcaaatc aacattttgt tttccgcctt acgaactaaa aatgttagag aaatgctaga
     8161 acgattgcag caagtaccaa atgtccatat ttatttgacg acctttgaac atccaaaagc
     8221 catcgaatta agtgaagtat cagatttagc aagtgagaga gtatcaatcg tttcattgtg
     8281 gcaattcgga ttagcagata tattggaaaa gatgacaaat gaagacgtat tagttgtgac
     8341 cggttcactt tatttcattt cagaagttcg ccatttatta atacaattag gaggagatca
     8401 tgaagtataa tgcagtgatt tttgacatgg atggtttgct gttcgacaca gagttagtgt
     8461 attacgaagc cacacaagta gtagcagaca agatgggttt tccttatgat aaagacctgt
     8521 atttaaaatt cttaggtgtt tcagatgaag aagtatgggc gaattatcat atgattttct
     8581 cggaatttgg caaaacaagt gtacaaagat tcatagatga ttcataccag gaaacgcttg
     8641 aacgtttttc gcaaggagcg gtacaaatga agcccggtgt gttggaactc ctctcttttt
     8701 tagaagaaca tcagattccc aaagttgtcg cttccagtaa ccaacgagca gtcattgaac
     8761 ttttattgaa gaagaacaat ctgtttgaac gatttgagac gattgtttct gcagaaaatg
     8821 ttcaacgagc aaaaccagac ccagaaattt tcttgttagc taatcagaag ctaggtacag
     8881 cgaagaaaga aacattgatt ttagaagatt cacaaaatgg tattttagca gcttctgcgg
     8941 ctgagatccc ggtagtcatg gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa
     9001 aaacagctgg ggttgtttct tctttacatg aaatccctgc attgttaaaa taaaaatcca
     9061 tctcttttcg tatcttattt tagataagat agaaaggaga tgtttttttg ggaaaacgat
     9121 tgatcaaaga agtacctact agctctttac caagggaacg tatggaaatt tatggcgcgg
     9181 aagctttatc ggatcaggag cttttggcaa tcttactgcg taccggtcaa cacccttata
     9241 gtgtgatgtc gattgctgga aatctgttaa aaacgtttgg aggactggcc tctttacgac
     9301 aggctacttt gcatgagtta gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa
     9361 tcaaagcctt gatcgaatta ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac
     9421 cggttcgtac cagctacgaa ttggcccagc aattgattct ggaaatgaaa gatcacaaac
     9481 aagaacattt ggtttgtatt tatttggaca cgaagaatca agtgatctta aaaaaaacag
     9541 tgttcatcgg ttcgctgaat cagtcagtcg ctcatcctag agaaattttc cattatgctg
     9601 tacgttattg tgcagcaagg atcgttttag cgcataatca tccttctggt aatgttttaa
     9661 ccatagtata ataaataata gtagttaaag tagtttaaga ggagatatta aagggatatt
     9721 ttactttaaa taaaaattaa caaggtttat aatttgtagt agttaaagta tttaaagagg
     9781 tgggaacatg tgaaggttca aaggatagaa gtggagaata agccgtatcc attgtattta
     9841 ttactagata aagaatacca gctaatagaa ccagtaatga aatttattaa atacttagat
     9901 aatactggta agtctcctaa taccattaaa gcatactgct atcatttaaa gttgctgtac
     9961 gagttcatgg aacagagagg tgttattctt aatgatatta actttgagtt gttagcagac
    10021 ttcgtaggtt ggttgagata tccttcagca tcaaatgtaa ttgatcttca gtcaaaaaaa
    10081 gccataagag aagaaacgac agtgaataca attttaaatg tagttatgag ttttcttgat
    10141 tatttaagta gattaggaga atttaaatca attgatgtat ttaaacaagc aaagggaaga
    10201 aatttcaaag gatttttaca tcatgttaat aagggtagat accaaaagaa tgtcttaaag
    10261 ttaagggtta aaaagaaaca gataagaaca ttgagatcag aggaagttaa gcaaattatt
    10321 gatgcttgtc atacgaaaag agataaatta attttaatgc ttatgtatga gggtggttta
    10381 agaatcggtg aagtgttatc gcttaggctt gaagatattg tcacttggga caatcaaatc
    10441 catttaacac ctagagatgt taatgttaat gaagcttata ttaaattaag gaaggaaaga
    10501 acaatacatg tgagtaaaga acttatgtca ctttatacag attacttgat atatgagtat
    10561 agtgaggaat tggagcatga ttatgttttt atttccttaa aagaaggcta ttttgggaaa
    10621 ccactaaagt accaaagtgt tcttgatcta gttagaagaa tagttaaaag gactggaata
    10681 gaatttacat cacatatgct tcgccacact cacgcaacgc agctaattag ggaaggatgg
    10741 gatgttgcgt tcgttcaaaa gagattaggt cacgcacatg ttcagacaac gttaaatacc
    10801 tatgttcatc tttcagatca ggatatgaaa aatgagttta ataaatacct cgagagaaag
    10861 gagcataaga aatgaatgct tctagtaaaa ggaaaattat tagtcagagt gagattagca
    10921 aaaaaatagc tgtaatgaat gaagaaatgc aggggttttg ggctaataat agttgggata
    10981 taagaaaatg tccacatcct tctgccatag aattaagtaa gaatcctgct ttaaggaatc
    11041 gttgggttcg ttttgaacgt gttaaaaatc tgtggttaag aacagaattg aaatattttt
    11101 atttttacca tttaaacaat ggaatatgga atgcaaaaac tgtctggatt agaaaaggaa
    11161 cagtaattaa taaaatgtta gatttcttag atttaaagta tcctagcatt acttcaatta
    11221 ctgaagttcc tattgaaaaa gcaatgacgg agtatagaac ttatttgaca aaacggggtg
    11281 ttagaattac cactactaat tataagatta ctgctaatca agaaaaaaca cctgtaaaag
    11341 ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag aacttttatt
    11401 ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct ttgccagatg
    11461 ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt cggaatacat
    11521 attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat agcttttcct
    11581 atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac atgaaattta
    11641 aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat ttaagtgaac
    11701 taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata ttggaaggac
    11761 tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata ttaatttatt
    11821 ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa ttcgtcctag
    11881 agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg actatgattg
    11941 ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc tgtctattag
    12001 aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa aaggagcata
    12061 tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat aaagtttcag
    12121 aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca ttaaaacaag
    12181 aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg gataaatcag
    12241 gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca agaatgatta
    12301 acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc ccagaaatga
    12361 caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact aaatttcagg
    12421 aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat gaagtcgatg
    12481 atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca aatggttatt
    12541 gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta gattgcactc
    12601 acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag cgtacagaag
    12661 agttattagc catagctaag gataaacaat ggcaaagaca agtagagact aatagccgtg
    12721 ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca atggataaac
    12781 aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa aagacaaggg
    12841 aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt aattttaatt
    12901 caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac gatattaggc
    12961 aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca aaacccaaga
    13021 aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta atggaattag
    13081 aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat ctgtataata
    13141 aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg ctaacggtca
    13201 gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat tgagtatcaa
    13261 tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat ttgattaacg
    13321 gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta tttggaagtt
    13381 caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc attaacagcg
    13441 atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga aaaaaactaa
    13501 cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt agaccacttg
    13561 aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca aaaagagaat
    13621 ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag gaaccaagct
    13681 atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt tctctatttg
    13741 gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga agagcaatta
    13801 aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat gtaattttaa
    13861 ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa gatgtcgctg
    13921 cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat atagctagaa
    13981 aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag gtgaaagcac
    14041 tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat tttattgcaa
    14101 taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca atttttggga
    14161 agaataaagc gtcctcttgt gaaattagag aacgctttat tactttaatt tagtgaaaca
    14221 atttgtaact attgaaaata gaaagaaatt gttccttcga tagtttatta atattagtga
    14281 catttgcatg cttcaaagcc tgtcggaatt ggtttttagt gaaaagaaca cgatattcac
    14341 ggtttaccca cttataaaca aaagatcgat actttttgta gtccttcttt gaaatcaatg
    14401 gttgatgtcg ttcaagaaca atcaatacag agtctacact tggcttagga tgaaaatata
    14461 gtggtggtac ttttttgagc atttttatat ccatctccac cattaatagt aaacccaaag
    14521 ctcgttgcag attttgcaat cttttcgcaa atcccttctc aacgataaga tagctatatt
    14581 tagcctgact ttcaaaggta attcttttga caatatccgt actgatgtta taaggaatat
    14641 taccatatat cttatagttt atatgttttg ggaaggaaaa ttttagaata tccgtttgaa
    14701 tcacttttat attctcagag gggtttaccg cttctttagt cacttgacat aagcctccat
    14761 caatttctat agcagtaact gatcgactca ttttgactag ctctttggta aaatgtcctt
    14821 ttcctgatcc gatttctatt acgttgtctt gtttactgat attcgtgtga ttcaatattt
    14881 cttttacatg ctttttagaa gtaataaaat tttgcgtgtc tttagggttt ttctggttca
    14941 ttataacctt ctccttactg gttataatga actgacatta agcgttcatt ataaccaatt
    15001 aattttgatt tggttgataa cgaactttat taataacaaa tatagaaaaa gtacccatac
    15061 ttttatctcc tttccatttt tttcattatg aatgagataa agtaatttct actactgcga
    15121 tactggtgca cataattgaa acctccttat gtaactgaat atgattgtat catcttttca
    15181 ttttagaata aacctttata tgtaattttg cacttgacac ttcagatgga cgttttcttc
    15241 aaaataatat gttttgaaac ttgataagag aagccactgt ctttactaaa aagtaaaggc
    15301 tgcgataaag gacagtgact ttaataaagt tgcactgttg tattgtcttt ggataaaatt
    15361 gtctaaaggg aagttttatc cccaacgaaa aaacatctaa agtggtaagt aatgaaagaa
    15421 tgtcgataaa gtaggaatgg gggatctttt tgaatacaat agaatcacag tttgataagg
    15481 ttgcagaaga ttacgatttc gtgaatgagc ttttgaatga ttattcattc tttgtgtcta
    15541 atatgtctcc aaagaaaggc agagcattag atatcggatg tggctcgggt ttgttagtgg
    15601 agaaattagc aagttattat gatgaagtgg tagggattga tatttctaat caaatgctcg
    15661 atcttgccaa atctaaacgt caactaacaa atacggtcta tctgaatatg aatgcagaac
    15721 aacttaattt taatgagaag tttgatttta ttgtaagccg aacaaccttt catcatttgg
    15781 atgatatagc cagcgtaata cagcaaatga aggaactgtt gaatgaggaa ggaagaatag
    15841 ttattcttga taatgtatct gaagtcgaga caccgcctac ctatgtttat aaattagggg
    15901 caatccaaga gtttttaccg cattgtttca aatttggaat aaaaaatgcg attagaattt
    15961 ataatcacaa cacatctaaa tcatggcttg agcatttagc ttcagataag tacctatctg
    16021 aacaaaatta ttacgattta tatgaaaagt tgttgcctgg atgccaattc cataaaatgg
    16081 gttgggcaat gggggttgtc tggacaaaat aacttaaagc tgtggctttg aaaagttgaa
    16141 tttgtgattg ttttcagagc tatcgggtgc tttagtataa ataacaatga ataaaaaatc
    16201 agcctgtatc ttatgttact attaagtaat ttaagataca ggttttacta tttttgaaat
    16261 aagtaccttc cacttcaaat acatgtattc aaagtgaaag tattgattta aatgattctt
    16321 ttgtaattta ctcttgataa tatgaaattc catcgcagca ggatatgaat tttaccaaaa
    16381 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    16441 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    16501 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    16561 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    16621 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    16681 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    16741 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    16801 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    16861 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    16921 agacaatttc tcttgtggca ggtatttttt tgtatgaaaa agtaaataaa tttaagaggc
    16981 tatgaaagag ccaccttgta tcaaggaata agaacaaaca aaaatggctt ctcatgtttt
    17041 tcatttgttt tctcttatat tcaggacaag gtgctgattt cataacctct tatttattct
    17101 tcatctggtg tcattaccat tggcaaaatc attggatgtc tttctgtacg ttcgaataag
    17161 aaaggctgaa gcgcgccagt catcgcttct ttcaatttat attctgaaca gtcaggtcca
    17221 ttcaaacatt ctcttaaagc gtggaataag attcgctgag cttcattgat catttctcct
    17281 gattctcgca tgtagacaaa tccacgagag agaatatctg gaccagccaa gatttctttt
    17341 ttcttgatat ccactgtcgc gacagctaat acaagacctt cttctgaaag caggtgacgg
    17401 tcacgtaaaa cgacattacc aatatcaccg atcccgctac catcgatata cacatctcca
    17461 gccacaaaat gcccagcagg acgcgcagaa tcgctagtta acgctaaaac atcgccattt
    17521 tccatgatga agcagttttc tgcaggaaca cctacatctt gtgctagaga agcatgtact
    17581 ttcaacatgc ggaattctcc gtggacagga ataaaatatt ttggcttcat caaacgaagc
    17641 attaattttt gttcttcttg tccaccgtga ccggaagtat ggatgttatt gatttttccg
    17701 tgaatcactt ctgcaccagc ttcagacagc aaattgatca aatgattgac acttgttgta
    17761 ttaccaggaa taggtgaact tgaaaagatc actgtatcgt ctggctggat ttggatttga
    17821 cggtgagtac cattcgcgat gcggctcaac gcggccatcg cttctccttg agaacctgta
    17881 cataggatca tcgtttcatt ggcaggcaag cgattgattt cgtgtgcatc tacaaatgtg
    17941 ccgtctggaa ctttgatata tcctaaacgt tgaccgttga cgatggcatt ttccatgctt
    18001 cgtccgaaaa cagcgatttt acgaccagtt gccacagcgg catctgctgc ttgctgcaaa
    18061 cggaagatgt tagaagcaaa gctggcaaaa atgatacgtc cttcgatttt tgaaaagatc
    18121 ttcaaaatag aatgcccaat cgttttttct gatttagtaa atgttggaat ctcagcattt
    18181 gtactatcag ataaaaggca caagactcct tcttccccga tacgagccat tttatgcaag
    18241 tttgctggtt cgccaacagg agtgaaatcg aatttgaagt ctccagtcgc tacgatattt
    18301 ccagaaggtg ttttcaccac gatccctaat gcatctggga tactatgggt cgttctgaaa
    18361 aagctgacag aagtctttct aaaacggatg atcgtatctt cattgatctc atggagctct
    18421 gcatctcgaa gcaagccatg ctcatctaat ttgtttgtga tcaacgctaa tgcgagagga
    18481 ccagcataga taggaatatt tgcttgtttc agcaagtagg gtattccgcc gatgtggtct
    18541 tcgtgcccgt gagtaatcac caaggctttt actttgtgta agttttgtat gatgtagctg
    18601 tagtcgggaa tgacatagtc aattccaagt aaatcgtctt ccgggaattt gatcccggca
    18661 tcaatcaaaa tgatttcatc ttggaattgt accccatacg tatttttccc gatttcacct
    18721 aatccaccaa tagcaaagac gcctgtctcg ttatttttga tggaaagttt catattaaaa
    18781 ctccactaat gagaaatcag cattttcttt ttcgtactct agatgacttc cttctagaag
    18841 ttgaacgaat tcaatattgt atggggtatt ttcttccaca cgttggcgag cgtctacatc
    18901 gctttttgcc tcaatgtaaa gagattttgt ttcttcccgt ttagggttgc gtacttttgt
    18961 ttcttgataa taaactttat agatcattgt tttgtctcct ttatctgcgt aaagcagtga
    19021 tttagctagt tgattttttt agacaacaca aaacgagggg acaagccacc tggtttttca
    19081 tatgctttga ctagaatact ggattcgagt caaagagaca agcctcgttt ttgcttatct
    19141 gttcggatat tttttcaacc gtaccttctg tataaaaaat atacaacaat taattgttat
    19201 tctagcataa aacagcaaat aagtatagaa atcaactaga ttgaaagaaa aaatcaatgt
    19261 ttatcatccc gctataatca taatgacttt tttatgagag aagtgttcta ttaataggct
    19321 cgttgtgcta tcatttttag taatgaaaat gtaacaaaag attaaaagga gatgagaaag
    19381 tgaaactaat gtggcattat accatgcgtt ataagaaatt cttgtttttc aatttcatat
    19441 gcgtatttgg ttttattttg attgaattag gacttcctac gattttagca cgtatgatcg
    19501 atgtaggaat ccgcaatgat gattttgatt atgtgaagca acaagggata tttatgatcg
    19561 ttattactgt aatcggtatt attttgaata tcttgttagg ttattttggt tcgaggatta
    19621 cgactaatat cgtagcagat attcgagatg atttgttcga aaagatccaa agttattcgc
    19681 atcaggaata cgaaaatcta ggcgtatctt cattgatcac tcgtacaaca aacgatgctt
    19741 atcagatcat gttgttcctg caaaatattt tacgtatcgg ttttatggca ccaatgatgt
    19801 tcattgtcag tttgtacatg gtcatgcgta caagtgtgac actcagtttg tatgtagtag
    19861 gtgcattacc tttactatta ttggcagtcg tagcgattgc taaggtgtct gaaccgttat
    19921 ctaaaaagca acaaaaaaac ttagataaaa tcaacagtat cttgcgggaa aatctttcag
    19981 gattgcgtgt gattcgtgca ttcgtcaatg aaaagtttga agaaaaacgt tttgctgtag
    20041 tcaatgagaa ttatgcttct agttcaaaaa gcttattccg tttgatggct gttgcacaac
    20101 cgggttttta tttcttattt aatatcgtaa tggtattgat catctggagc ggggcagtgc
    20161 aaatcgatgc aggcgagctt cgtgtaggag atttgattgc gtttatcgag tatatattcc
    20221 atgccttgtt ctcattcatg ttatttgcta gtgtgttcat gatgtatcca agagcagctg
    20281 tttcagctgg acgtatccaa gaagccctag atgccaaacc ttcgattgta gaagatccaa
    20341 atggtatatc tgatacaaaa actaaagggt atatcgaatt tcaaaatgta acatttgctt
    20401 atccaggcca ttcgcaagag ccagtgatac gaaatgtcag ttttacagcc tctccaggag
    20461 agaccgttgc ttttattgga agtaccggta gcggaaaatc tacgttgatc caattgatcc
    20521 ctcgctttta tgatgtgagt gaaggaaaga tcttgatcga tggagtagac gtgagagatt
    20581 atcggctaag caagttgcga gataagattg ggtttattcc gcaaaaagcg ctgctattta
    20641 ctggaacgat tgcagataat ttgcgatacg ggaaagaaga tgctacgcaa gaagaaatgg
    20701 aacgtgcagc tgagattgct caagcttcag aattcatttc tcgaaaacca gatggattta
    20761 atgaacattt atcagaaggt ggttccaatt tttcaggtgg acaaaaacaa cgattggcaa
    20821 tagccagagc catcatacgc cgtccggaaa tctatatttt tgatgacagc ttttctgcat
    20881 tagattatca aacggatgcg aagttacgtg ctcgtctgaa aaaagaaacg acagaatcta
    20941 ctgtattgat cgtagcacaa cgtgtgggaa cgatcatgca tgcagataag atcatcgtat
    21001 taaatgaagg agaagtcgtc ggtatgggga cgcatcggga gttgttggaa aaatgcccga
    21061 tttattatga tattgccgct tctcaattat caaaggagga attagcatga aaaaaacctt
    21121 ttcttcctta aaacgtttag ctcgatatat cagaccgtat cgtgagactt ttcttttagt
    21181 gatcctattt acagttctga cagtggcttt caacgtggct ttgccatatg ttacaggact
    21241 tcctactaca gaaatcagcc gtaatattgc caatggcgaa tcaatcaatt tttcttatat
    21301 attcaaatgt ctcatttgga tcacagttgt tggaataggg tattgtattt cgcaattact
    21361 ttctggtgtt ttgatgacaa atgtcgttca aagtgccatg catgatttga gaagagacat
    21421 agaagaaaaa atcaatcgtc ttccggtttc atatttcgat aaaaaccagc aaggaaatat
    21481 tttgtctcgt gtgacgaatg atgtagatgc agtcagcggt gctttacaac aagcttttat
    21541 tggtatcgta aatgcgatat tgggaattac gttggctgca gcaatgatgt tttatatcca
    21601 gccgatgatg gcgctgatct ctatgatcat gattccaaca tcgatctgga tctcaaaacg
    21661 agtgatcaat gcttcgcaga aatattttca aagcatgcag aattctcttg gtgagctaaa
    21721 cggctacgtc caagaaaata tgacaggttt tagtgtattg aaagtatatg gacgcgaaaa
    21781 agaaacattg gaaggattca aaaaagttaa tcacagcttg aaatactttg ggtttcgtgc
    21841 agcattcatt tctggcttga tgatgcctct agttcaatta actgcctatg ctacttatat
    21901 tggtatggct gttctaggaa gcttttatgt tattactggg gcgatcgttg tcggtcaact
    21961 acaagcattc atccaatata tttggcaaat cagtcagcca atggggaacg tgacacaatt
    22021 gtcttcagtt ttgcaaagtg catcagcggc aacgaaacgt gtattcgaga ttttagatga
    22081 gccagaagaa aaagaaaaca aagtagatgc ttcactgcca gaaacgattt taggagatgt
    22141 gacattcgat catgtcgatt ttgcatatga tccgaagaaa ccattaatcc aagatttgaa
    22201 ttttgaagtg aaagctggtc aaaccgtagc tgtggtcggt ccgaccggtg ccggcaagac
    22261 gacattgatc aatctattga tgcgttttta tgacgtagac aaaggagcaa taaggattga
    22321 tggaatcgac accaaatcaa tgagtcgcag tgatgtgcgt tcgctatttg gtatggtctt
    22381 gcaagatgca tggctttatc aaggaacgat tgcagataat atccgcttcg gaaagcttga
    22441 tgcgacagat tatgaagtag ttgatgctgc gaaaacagca aacgtagatc attttatccg
    22501 aacgatgcca gatggatatg atatggccat caattctgag ggagacaatg tgtctttagg
    22561 acaaaaacag ttgctgacga ttgcccgagc agtggtctcc gatcctaaaa tcttgatttt
    22621 agatgaagca acaagttcag tagacacccg tttagaagcg ttgattcaaa aagcaatgga
    22681 tcgcgtgatg gagggacgga caagtttcgt catcgctcac cgtttatcga caatccgtga
    22741 tgcagatctg attctagtga tggatcaagg tcagatcatt gaaaaaggaa cgcatgagag
    22801 tttattggca caaggtggat tttatgaaaa actgtataac agccaatttg ctgaggaagc
    22861 agaataagtc atataagaat aaaacagttt aaaacttatt ttttgttctc tattcttgat
    22921 aatggtaaag taaagttatc aagaaaagtg aggacacgga gatggaacag tttttaaatg
    22981 aaaaaattac tgaagccaaa agagaagttg atcgtttaac gcacgtctgt cagacgaagc
    23041 cttttgattc agctgatttt tttgaaaatg agtttcaaat cgaacgcttg gcagcacaaa
    23101 tagaagtgta cgaattaatt ttaaaaaaag taatagaaga aaatatagat gtacaataaa
    23161 aacaggtaaa acgtcagtta ccagatactg taaatagaca tctggcctga gcaggtttta
    23221 cctgtttttt ttcttatgaa ataaatatat gctatgatac ctttattcaa taggattagt
    23281 ttctacaaaa aatgaaagga gaaaaataaa tatggaacgg tttatagcat caaatgccac
    23341 agtgattggt gatgtgacat tgtcagaaga tgtgacgatc tggtatcaag ctgttttacg
    23401 cggggacagt aattggataa aaataggaca aagaacgaat atccaagatg gtacaatcat
    23461 ccatgtcgat catgatgcac ctgtagatat tgcggagaat gtgacggtag gacatcagtg
    23521 catgctacat ggctgtacaa tcgaaaaagg ggcgctgatc gggatgggga ccacgatatt
    23581 gaatcatgcg gttattggtg agaatagttt gattggtgct ggatcattag taacagaagg
    23641 aaaagttatt cctcctaatg tcttggcttt tggtcgccca gctaaagtga tccgtccttt
    23701 gacaaaagaa gaaatacaaa aaaacaaaga aaacatccag cattatgtcg agataggtcg
    23761 gatgcatgcg caaggaaaat tcaaggagca aagataggca gtagcatcca taaaataaaa
    23821 aaggatggtc agtttgagtt gaccatcctt ttttatttgg tttagataag agagcttaaa
    23881 aaagcttcct ttgaaacaga ttaatttaat gaagaactgt tttccatgac ttcatcgatc
    23941 aatccatatg cttttgcttg ttcagctgtc atatagttgt cacgatctgt atctttttca
    24001 atcacttcaa gcggttgtcc agtgcgttca gctaaaattt tgtttaagcg ttcgcgtgtt
    24061 tgcaaaatat ggcgtgccgc aatttcgatt tcagttgctt gcccttgagc gccgccaaga
    24121 ggttgatgga tcatgatctc agcatttggt aaagcaaatc gtttaccttt tgtaccagct
    24181 gtcagcaaga agcttcccat tgaagcagcc atacccataa caattgtttg gacatctgct
    24241 ttgacaaagt tcattgtgtc gtaaatagcc atacctgctg taactgaacc accaggagaa
    24301 ttgatataaa gatagatatc tttttcagaa tcttgagcgt ctaagaaaag caattgggcg
    24361 atgatcgaat tagcaagatc atctgttact tggccgctta acatgatgat acggtctttc
    24421 aataaacgtg agtagatatc gtaagctctt tcacctcgtg aagattgttc aatgactgta
    24481 ggaattaaat tcataataga gttcctcctt gatttcgttt agaaatagtg taccataatt
    24541 tagtttctaa tacctaaaat gtagtataca tttttggtca aaaaaggtca aataaaaaac
    24601 ttaggcaaat tagtcgcata aacccgcttt atggtatgat aagcagagaa acaaaacaag
    24661 gaggaacaac agattgatag ttggtattac tggccttatc ggagtgctga caggtgctgt
    24721 agttgtctat atcggcatga tactgcaaat gcgtcacttg gaaaataaag aacagcgtag
    24781 acgtgaattg gaattgaaag tcaaagaaat cgagacgctg aatcaattaa ataaaaagat
    24841 caacgagatc ttgcaaaaac ggacaacgct gatggaagaa tatgtcagct ttgatgcatt
    24901 tgatgattgt tatatcacga ttgacgattt tgcgtatctc caatcatttt cagcgcaaaa
    24961 tagtttttat ctgccaaatt atattttaga cgagctattt aagaatatct cccatcgaaa
    25021 agtgatttta tctccagatg aaacaatgaa gattggtgga tatgcttata aaggcggacg
    25081 cgtgatttta gagaatttta cggatagtct gactgaaatg atcaatgaaa aaaaagctca
    25141 gttgaagact atgacgcgcc agccgattgg tttcttctcg aaagatgatg tttggagctg
    25201 atattttgaa gcattgccgt tgcacatgag agcgtggtaa tgctttcttt ttttcttaat
    25261 gatacaatag aactgaacgg agtgaagaaa gatgttacaa atctatgccc atcgcggaag
    25321 cagcggcacc cacccagaaa atacgcttcc tgcatttgca gaagctgtac gagtaggtgc
    25381 agatggtatc gaactagatg tccatctaag caaagatggt tatttgatcg ttatgcacga
    25441 cgaagaagtg gaccggacga ctaacggtaa agggctgatt cgagaaaaaa cattggaaga
    25501 actcaaaaaa ttgaatgcag ggggcaaatt cagcaaagaa tttccagcag ctaaagttcc
    25561 tgaattgaga gaagtgttag gactgctcct ccaaaaaaat taccgaggga tgctaatgat
    25621 tgaaattaaa actgatcagt atgaatatcc cggtatcgaa gaaaaattag ccgaaacatt
    25681 aaaaagtaga gattggcctt ttcaacatgc atattgcagc tttcatttgg attctttgga
    25741 aaaaatcgca ttactggaac cggagataca gctggactta ttgatgagta cttctgataa
    25801 aaagatccaa ctagcagcag aaacccctta tatagaagga gtacatccaa aaaatacttg
    25861 gttgttcgaa catccagtcc aggcagctga ttttccaaaa gcaattcgtg catggacgat
    25921 taacgatgaa gaagaaatgg ccaaatgtca agaactcaaa atagatggaa taataactga
    25981 ttttccggag aaagctcttc ggtggaaaca atcaacgaaa agtaggtaaa atatggaaaa
    26041 agtattagtg atcgtcggtc cgacagcagt cggtaaaaca gcgctaagtg tggaactcgc
    26101 acaactattt ggaggagaga tcatcagtgg tgattcattg caaatctata aaaatctgga
    26161 tatcggtaca gcaaaaatca aagaagaaga gatgaataat gttccgcatc atttgattga
    26221 tgtgatcgaa cctactcaac gttactcagc cgctgatttt cagcattcag gtcgacaatt
    26281 gattaccgag attgcgaacc gagggcacct acctatcata gctggtggta cgggtctcta
    26341 tatccaatcc cttctatatg attatcagtt aggagcgaca gaagaagcga atcctgagat
    26401 tagaaaaaag tatgaaacgt tagctgaaac aattggttcc caagcattat ggaatcagtt
    26461 gaaagaaaaa gacccggcag ctgctgagaa aatccactgg aacaatcaac gcaaagtgat
    26521 aagagctctg gaagttttag agacgacagg acatagtatc acggcaccta aagaagagcc
    26581 aaagcaattg tatgattacc tcatgattgg attagatacg aagcgtagta ttctttatga
    26641 acgaatcaat caaagagtcg atttgatgct agcagaaggt ttagtagcag aagcgcgtaa
    26701 ggtgtatgag cttggagaag tccaggccag tcaaggtatt ggatataaag aatttttccc
    26761 ttattttgaa gggaaagaaa cattggaaga agcaacagaa caagtcaaac tacattcgag
    26821 aagatacgcg aaacgtcagt tgacgtggtt tcgtaaccgt ttggatgcac agtggtttga
    26881 tcttataagt gagccaaatc aaaaaagtca gattgagcaa acaatcaaaa aatggctgga
    26941 ggcggaataa aatggaagaa aaagtgatca tcgtaggggt agaaaccgaa gcaaaccagc
    27001 gttacttttc agagtcaatg gaagagctag ttcagctgac gaataccgca tcaggagaag
    27061 tcgtattcac aattacacaa aagagaccac aagtggatcg gcaaacgatt atcggtaaag
    27121 ggaaattaca agaattagtt caacaagcgg atgcgcatga agcggatttg atcatcttta
    27181 atcatgagct aactccgaga cagagccaac tgattaccga tgctgttggt cttccggtaa
    27241 ttgatcgcgt gcagcttatc ttagatattt ttgcgatgcg tgctcgttcg aaagaaggga
    27301 agctgcaggt tgaacttgct cagttagaat acctgctgcc tcgtttagta gggcaaggga
    27361 aaacattatc tcgtcttggc ggtgggatcg gaacaagagg gccaggtgaa acaaagttag
    27421 aaacagaccg ccgacatata cggaataaaa tttttgctgt taagaaagaa ttgaaagaag
    27481 tagaagctca tcgagagcgc aatcgccaaa aaagaaaaaa tagcgagatt tttcagattg
    27541 gacttattgg ctacacgaat gccgggaagt caactatctt gaatctattg acacaagcgg
    27601 atacttattc gaaagatcaa ttgtttgcaa cgttagatcc attgactaag agatggcgtt
    27661 ttgctgaagg atttgagatt actgtaacgg ataccgtcgg gttcatccaa gacctgccta
    27721 cgcaactgat cgatgctttc cattctacat tagaagaaag tcaaaatatg gatcttttgc
    27781 tacatgtagt cgatgctagt tctccagatc gtattttaca ggagcagact gtcttaaaat
    27841 tgatggatga actgaatatg aaagaaatgc cgatactgac tgtttacaat aaggcagacc
    27901 agatcgaccc agcgatgttc acgcctacgc tattcccaaa tgtattgatt tctactcaga
    27961 cgaaagaagg taagatggcg ttgatcgaag caatcaaacg tcagctgatg gaactaatga
    28021 ccccttatac actttctgtg ccaagcaacg aaggacagaa attaagcgag ttgagaagac
    28081 aaacaatggt tttggacgaa gcgtacatgg aagaaacaaa cgaatatgaa gttaaagggt
    28141 ttgcaaaaac caattcaaaa tggttaagaa aaaaccaata aaattttatt tttttaa
//
LOCUS       DAJSBM010000027        27998 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJSBM010000027 DAJSBM010000000
VERSION     DAJSBM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.027"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..850
                     /locus_tag="ONT67_001449"
     CDS             2..850
                     /locus_tag="ONT67_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2355199.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            950..1384
                     /locus_tag="ONT67_001450"
     CDS             950..1384
                     /locus_tag="ONT67_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2355200.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            1564..1974
                     /locus_tag="ONT67_001451"
     CDS             1564..1974
                     /locus_tag="ONT67_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2355201.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            2031..2471
                     /locus_tag="ONT67_001452"
     CDS             2031..2471
                     /locus_tag="ONT67_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2355202.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            2574..3653
                     /locus_tag="ONT67_001453"
     CDS             2574..3653
                     /locus_tag="ONT67_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2355203.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3779..4920)
                     /locus_tag="ONT67_001454"
                     /pseudo
     CDS             complement(3779..4920)
                     /locus_tag="ONT67_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            5068..6621
                     /locus_tag="ONT67_001455"
                     /pseudo
     CDS             5068..6621
                     /locus_tag="ONT67_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6729..7811)
                     /gene="uxuA"
                     /locus_tag="ONT67_001456"
     CDS             complement(6729..7811)
                     /gene="uxuA"
                     /locus_tag="ONT67_001456"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2355204.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(7802..9448)
                     /locus_tag="ONT67_001457"
     CDS             complement(7802..9448)
                     /locus_tag="ONT67_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR2355205.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            9632..10294
                     /locus_tag="ONT67_001458"
     CDS             9632..10294
                     /locus_tag="ONT67_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2355206.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            10438..11001
                     /gene="ahpC"
                     /locus_tag="ONT67_001459"
     CDS             10438..11001
                     /gene="ahpC"
                     /locus_tag="ONT67_001459"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCR2355207.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            11011..12540
                     /gene="ahpF"
                     /locus_tag="ONT67_001460"
     CDS             11011..12540
                     /gene="ahpF"
                     /locus_tag="ONT67_001460"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR2355208.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            12697..13047
                     /locus_tag="ONT67_001461"
     CDS             12697..13047
                     /locus_tag="ONT67_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCR2355209.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            13146..13676
                     /locus_tag="ONT67_001462"
     CDS             13146..13676
                     /locus_tag="ONT67_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCR2355210.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            13830..14981
                     /locus_tag="ONT67_001463"
     CDS             13830..14981
                     /locus_tag="ONT67_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCR2355211.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(15090..17129)
                     /locus_tag="ONT67_001464"
     CDS             complement(15090..17129)
                     /locus_tag="ONT67_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCR2355212.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17187..17744)
                     /locus_tag="ONT67_001465"
                     /pseudo
     CDS             complement(17187..17744)
                     /locus_tag="ONT67_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(18283..18489)
                     /locus_tag="ONT67_001466"
     CDS             complement(18283..18489)
                     /locus_tag="ONT67_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355213.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(18505..19119)
                     /locus_tag="ONT67_001467"
     CDS             complement(18505..19119)
                     /locus_tag="ONT67_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR2355214.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(19266..19949)
                     /locus_tag="ONT67_001468"
     CDS             complement(19266..19949)
                     /locus_tag="ONT67_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2355215.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(20058..20516)
                     /locus_tag="ONT67_001469"
     CDS             complement(20058..20516)
                     /locus_tag="ONT67_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR2355216.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(20600..22363)
                     /locus_tag="ONT67_001470"
     CDS             complement(20600..22363)
                     /locus_tag="ONT67_001470"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR2355217.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(22584..24044)
                     /locus_tag="ONT67_001471"
     CDS             complement(22584..24044)
                     /locus_tag="ONT67_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355218.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(24252..25178)
                     /gene="glsA"
                     /locus_tag="ONT67_001472"
     CDS             complement(24252..25178)
                     /gene="glsA"
                     /locus_tag="ONT67_001472"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR2355219.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(25201..26628)
                     /locus_tag="ONT67_001473"
     CDS             complement(25201..26628)
                     /locus_tag="ONT67_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355220.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(26659..27873)
                     /locus_tag="ONT67_001474"
     CDS             complement(26659..27873)
                     /locus_tag="ONT67_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCR2355221.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         8198 a         5326 c         4996 g         9478 t
ORIGIN      
        1 aatgaaagaa acgagacaat ttatatggac atacattaag attatttttg ccctcttcat
       61 attaggagtc gctatcaata tgtttttagg gcctcatcat attgctgctg gaggtgtcag
      121 cggtctagga atattactgg aagctgcttt aggctttgac cgagctatag ttattttagt
      181 gttgaatatc gttatgttgg tactagctct tctattttta ggtaaaaaac ccttttttaa
      241 agtacttttc ggaagcttgg tttttccttt aatcattgca atagttccag aaacaatgat
      301 tacttcagat cggttgttgt ctgtcatttt tggaagtgcg atttttgctt taggagttgc
      361 gattttatat aaaaacaact cttctagcgg cggtacgacg atccctcccc tcatttttaa
      421 aaaatatttc cacttgaata cttcgattgg cttatttttt acagacgcat tcgttgtttc
      481 tcttaatctg tttatttttg gaatagaaga gtttctttat gctattttat ctattgcaat
      541 cacttcaatt gtcatgactt atatcgaaac aggtatcaat cgaaagaaat ctgttatggt
      601 catgagtgaa cattatttgg aagacatccg ccttcgctta agtgctgaga ttggacgtgg
      661 ccttaccctg ctagaggcaa aaggcgggta taatcgaaag tcaaaagagg ttttattgat
      721 cgttgtgact gatcatgaat tttcccgaat gaaaccattg atagaagaaa tcgatccaag
      781 tgcctttgtc attgtcagca gcgtagcaga agtaatggga cgcggattta cttatcatcc
      841 aatcgaatga gttggtaagt aaatccgcta tttttttgaa tgcgttttct ttagctggta
      901 taataaaaga atctagtaac tgttcgactg aaaaggagag gagatcttta tgcataaagg
      961 aacggaaatc gattttgtat taaatgatag tttgaaagca ctagaactgt atcaaacaat
     1021 ctttgatgaa atacaactga ttgaggcaac agattaccct agaggcaata atgaagtcat
     1081 ttttaccttg tatggtacga gattccatat gctggatgaa aacacgtctc ttggtttgac
     1141 tgctcctact gctgaacatc caaatacgat ctggttcaat atcatcgtct ctgatatcga
     1201 ggacacacat aataaagcat tacgaaatgg ctgtgtagag attcaagaaa tgacagaaat
     1261 gatggacttt ggtgtgacta actcaatgtt cagagatcct tttggttatt tatggatgct
     1321 tcatcaggtc catcgtgaag taatttttga agaaagaaaa caactttttg agaaatatat
     1381 gtaatgttta tgatgagatt attttttgtc tctgatattt tttgtatatg tttgctattc
     1441 gagaaagcgc gtctgtttca tgtgctttct cttatttatc ttatgccaat ccatttcttt
     1501 gctttccaca attatattgt gtaaaattca attgtataaa atataattaa aggtggtaac
     1561 tttatgaaaa aaatgtattc tacgaaaatg atcaacacag gcggacgctc tggagaagtg
     1621 cataatcccg atcgtacatt cgaattgaat attgcagctc ctggtaaacg cgtcgaaaat
     1681 gcaaccaacc ctgaacaatt gtttgcagct ggtttcagtg catgttttaa cagtgcatta
     1741 gaattgatca aacagcaaaa aaatatctct ggggcttcta ctgtcagtgc acaagtttcc
     1801 ctttattcag aaagtgaaac gagctttgtc ttaggtgtag agattgaagg atctatcgaa
     1861 ggtctttctc tggaagaaac gcaagaatta ttagaggcag cgcataaagt gtgtccgtat
     1921 tcaaaagcta cagctggaaa tatagaagtt actctgaaag cagttagtga atagactttt
     1981 ttgtgtaaat ccgctatgat acactatagt aaaacagcgg aggtggaaaa atggaagatc
     2041 tatatttagt taatcaagta tgttttcctt tatatgccgc ctctaaagaa atcattagac
     2101 actacactcc cctcctcaag ccgctgaatc ttacctatac ccagtatctt gtgatgttag
     2161 ttttatggga aaagaaaaag acttcagtga aagagctagg aaatgatttg tgtctggatt
     2221 ctggtacgct gactccctta ctaaaaaagt tagcagaaaa aaattatatt aatcgtcagc
     2281 gtgaaacggg tgatgaaaga atcgtaaatg ttacgctgac tgctcaaggt gctgcgttgc
     2341 aaaaacaagc agaaggtatt ccacaaaaga tcaagtctta tcttccactt gaacaggaag
     2401 aattggatac actgcaacat ctgacccgaa aaatgctaga taattttcat caagaccaga
     2461 aaaagggctg aaactgctag atgaacgatt ttatgaggaa aaccaactca taagataacg
     2521 tcatttagcg gtttttcttt ttgctctttc ttattttcat attccatcat ataatgaata
     2581 acgtgtttat tttttgttat ttggaggttg tcgatttgac cgtttatcag cgttttttgg
     2641 cgaatgaaaa aatacgccgt ctcactgttt tattatttgt cgtttttgtt cttttcttag
     2701 ttagaagtat gattacaacc attttgctca catttatctt tacttatttg atgattcatc
     2761 ttgttcaaat cattcagcga tttgtccgaa tccctacagg tgttttagct atcatgattt
     2821 atacactgat cgtttacctt atctatctgg cattcacaaa gtatatcccg atgcttattc
     2881 atcaaacatt tgacatgatc cagtccgtta ttaattttta cgagcatcag ccaaaagatg
     2941 cagatcctgt tttacaatat atccatactt atattgagcg aaacgatttt tttgaacagc
     3001 ttcaaaatgg cgcttcgatt gcgcttggct atttacagga tataggaaaa atggcagtgg
     3061 cgtttgctat gtcattcatc ttaagtttct tttttatgat tgaaaagaaa aaaacaattg
     3121 tattctcaag attgttttta aaaagtgaat tttcttggtt ctttcaagat atttactatt
     3181 ttgccgataa gtttgtcaat acatttggtt tggtattaga agctcaattt gttgtcgctc
     3241 ttttaaatac gttgctcact acaatcgcct tggctgtatt tggctttcat cagctgctga
     3301 gtttagcgat catgattttc attttaagtc tgatcccagt agcaggtgtt atcatttctt
     3361 gtattcctct atctttcata gcttattccc aaggaggcat ccgagatgtt gtttatatcc
     3421 tgattacgat tttgattgtc catctaattg agtcttatgt gctgaatccg aagttgatgt
     3481 ccagcaaaac agaactgccg attttttata catttgtggt cttacttgtt agcgaacggt
     3541 tctttggcgt ttggggcttg attgtcggca ttccgatctt taccttcttt ttagatgtat
     3601 tgaaagtgaa aaagatcccg aaccatcttc caaaagaaac agatatccct taaaataaat
     3661 atgaagaaag ataaagtatg aacgacccca aatatcagat ttcaatctgg tattttgggg
     3721 tcattttctg gatatttctt tttatttgaa aaatgcgttc acagacatta ttcaaatgtt
     3781 agagagatct gttttgttta aatctttttg cagaaagcat tgttgctata cttaatagaa
     3841 tgattccgct ccacagtata actttgtttt gtgactctcc ggattttggt agctgtttat
     3901 ttttcgtttc aggacagcaa aggttgtatc cttgctactt tttaaggttt cagctgattg
     3961 ggcgctatca aatattcgtg attttttatt cagctgctct tcatttattt ttagaattgc
     4021 tagatttgat tttttactct ctttttttcc atcctcttga attgtttttt tgtttatttt
     4081 ttctggtatt ccttggtctc ttgcgtccgt atctggttct ggagtcacat ctggtttttc
     4141 ttctggatct acctctggtt tttctggatt ttcagtttca ttatctgtgt ctggatttgt
     4201 ttccggtact gtttggtttc cagaatccgt atctggttct ggagttacat ctggtttttc
     4261 tccaggggtt atttcaggtt ttgatttatt gtctggctct ggttgagttt ctgtatcggg
     4321 ttcttctcca ggttctattt ccggtttctg ttctccgtct ggattttctg taggcggtac
     4381 ttctggctta ttttcatttt ctggtttatc tgttggttct tcaacaagat ctcctcctga
     4441 gtcgatggct gaatcttgct caattgtctg ccgctgacta tacggcgtta aatgatgatc
     4501 gttcggtagt ccatctgata ccttgacagt aaaaagcgct ggttttaaca agtcctgatt
     4561 taatgtaacg gtgaaggtaa atatcgcttg cttgtgttgg atagtcaaag tgttctcgtc
     4621 atagccaatc gacaaagtgg gatcaacatt ctcaaatgta acagctgatt gtagtatagg
     4681 tgctaagtct cccgaatagt caatttttgc tttgatggtc gctgtttgtc cttttcttaa
     4741 ttttgcgact cctgaattga aggatgaaaa ttcatcctga aaggtaattc gattgacgtt
     4801 ggcccacttg gtttctgtcc atttggctgt ttctgtctcc tctgtgtgga ctgtctgatt
     4861 tatagtaagc gataagctta aggctgcaaa tgttgatatt aagatggttg aatatctcat
     4921 aactctctca ctccttgatt tttttgtatg caagaaataa gatagtagag attctaccta
     4981 tatgtttact gtttttttgt ctaaatttat gaaaaaaaca gtaatatttt caagttttaa
     5041 caatttaagt aaaaaggagc ctctttcatg caaactcaag aagcgattaa aacgtttatc
     5101 ttgaagaaaa aaagcaatac aaaattagat atttttcttt tcctatctga acatcgtttc
     5161 tttattacta cacaatattt agcagatcat ttccatatgt ctgaatcaaa ttttttacta
     5221 tacatcaaag aattagaaca agactttgag cgtctcaatc taacagaact gcatattgat
     5281 aagcaaaaac cttttttaaa gttgaatttt gaaggaattg atccggctta ctgctattat
     5341 cgtctatttg gaagatactg caacgaatca gtcagttatc agatcttgac ttctttgttc
     5401 agctgtcaga caaattcgat tatcagtttc agtcagcaaa caaactatag tgcctcttat
     5461 ttgtatacga aaatgaaaaa gattaatgca tttttagcat agtacggttt gtctatctcc
     5521 ttttctaata atggcagaaa gtctttttct ggaaaagagg tacaaattca atatgcagca
     5581 ttagatattt attggaacat cttttcaagt actgctacag aattttatga tgaagatccg
     5641 caagtagtgg cttttatgat gaatactttt tttaaaaaag aagtacttga ccgactaaat
     5701 agcggtatga tagataaatt atattttcta ttaaaaatat gtaaagagaa ttttcctgca
     5761 acttctgtga aagacgtaca aaaagaattc acagagtatg aatatattga cttttttatt
     5821 gatctctctg tagatatttt gcgacctaat ctgccaattt gtcgcgaaca acgcatctta
     5881 gtgaatattt tagctcgact ttctatcacg aaaatcgaat cggaagaaac aagcttggag
     5941 caatacaaac ttttaaaaaa agcaaacgtt cctcatgttc tttattcaag agaaatggtg
     6001 gagtcccttt gtactgtatt tgatttgttt atacctgaaa aagaaaagat tctttactca
     6061 ttggcattcg cacgaaataa actttataat ggatttctta acttcaatca accaaatacg
     6121 ccccttccta ctttcatgct ttatcaagaa catagcgtat tgaaagaagt ccaaaccgcc
     6181 attgaacaat tttatatgga tttcagagaa caaaatcaac acttgttacc agttatttta
     6241 gaaaaggaaa atgtgcaatg gatggttgaa gacctagtac atttgtatga tcgctacaaa
     6301 aaacagccgc gaattgtgat tggtgtgaac tatacgcgtg atttttatat cagtaaagat
     6361 ctgatcgtta agatagaaca aattttttca aatgaatcga tcaaaataca aaatgatttt
     6421 atggagaact gcaacatcgt tatcagcgac tgccctttga aacaattgcc ttctcatatc
     6481 aaaaaaatct atattttaaa aggaatcatc acaccagaag actggaaaaa agtgattatg
     6541 aggatctcag aatatatctt tgaattaaaa gaagaagcac tcatggatca atcgaaaaaa
     6601 gaaaatcaag ttacttccta gaccaaataa gatacataaa tccgaatgct tattaacaac
     6661 ctccggattt atgtatcaag aatttgaaca tccttcttat ttacataata aaattacagt
     6721 ttttttcact aattatcttt acttttgttt cctttttcga ttgcttccca taatccattt
     6781 agataaacag cacctaatgc tcgatcatat aagccgtatc ctggtcttcc tgtctcattc
     6841 cagatcattc ttccatgatc aggacggatg tatccgttaa atccgttgtc atacattgtt
     6901 tttagtatgc ggtacatgtc taatgaaccg tattcggatt tatgtggtgc ctcatagaaa
     6961 tctcgattag ctaaaaattt aatatttcga acatgtgcaa atgggattcg atctctttgg
     7021 caaaattctg ctaaaatccc atatacatca ttttctggat cttctgcaat actccctgta
     7081 caaagagtga ttccgttata agggctgtct actatgcgac acaaccaatc taaatcttct
     7141 cgattcttta cgattcgagc taatccaaaa atcgagtatg gcggatcatc tggatgtaca
     7201 gccattttga cccctacctc ttcacatgtt gggatgattg attggagaaa ataggcaaaa
     7261 ttttctcgaa gtttttcttc gtctactgat gcataggctt caagcaagga tttgatagga
     7321 gctaagcgct caggttccca tcccggcaat ccaaattcat tgctggaatg ttcgatgcgc
     7381 gcgacgattt ctcttggatc atctggaata tcatcagata taaaagctaa cgtggatgag
     7441 ccgtcttcta agcggtagtc aaggtcagat ttcacccagt caaatacagg cataaaatta
     7501 tagcagatga cttctattcc atattctgcc aagtttcgga ttgtctgctt atagttttcg
     7561 atatactgct cacgggaagg taatccaatt ttaatggcat catgcacatt gacgctttca
     7621 attactttta gtgttaaccc acgagactca atttcttctt ttagttcttt gatcctctcc
     7681 ctaggccata cctctcctac tggtatatcc atcaatgtgc ctacaatccc tttgaccgca
     7741 ggaatctgtc tgatttcatc taaggtaatc ttttcttcct gtgatccgta ccatctaaaa
     7801 gtcatttcca tgataaattt cctccatgtt cttgtaagta atcctgtaat gtttttcgta
     7861 cggcatttgg tcctgctacc atcttgatga acatttcttc tatttttctg ccaagctctg
     7921 cttggtaaag atctacacca aaaatttctt tatttgacaa aataggagcc attttctcat
     7981 gaatatgtga tgtatcttgc gtacccagtt gaatatcgga aagcttatgc tgaaggtctg
     8041 tcaatagtgg gtctggactc gggatgaatg gttttccttg atcatcaatg ccaataaggt
     8101 atcgaagcca agcagcgaga gtcaatggta tgaatacaag tgtctgcggg tctttaactt
     8161 ctatttccat atatttttta atcgtctcac cataacgaat cgccaccttt tgagaggtat
     8221 ccgtggcgat tcgctgaggc atattaggaa tatttggatt agaaaagcgc acagtaatta
     8281 cttcttccag aaagatcttt ggatcaatga tacctggaga ctccacaact gggaggcctt
     8341 cattgtaccc gatttcagaa actagcagcg ataaatctgg atctttcatc tcctcagcta
     8401 tgctcctata ccccagtaaa caacccataa tagctaaaga cgtatgcaaa ggattaaggc
     8461 aagtcgtcac cttcatcgta tctgctttat ctaccgttgc acgatcagtc aagaataccc
     8521 cagctttttc gaatttcgga cgtccattgg gaaaatgatc ttccatgatt aaataatgga
     8581 tgtcctctgt attgacaaat ggagcaatat tatttccttt tgcggtttgg aaaatcgaca
     8641 tatttgaaaa gccctctttt gccaacattt tgctaactga caaggaagga ttcggggtga
     8701 tacgatcaat catcgtccat ggaaacgcaa caatttcttc attcgataag tattggataa
     8761 atgtattttg tacataacct tccttttgcc attcttttgc gatcgttagc aaagaagtac
     8821 gtaattgact accgttttgt gaaaaattat ctgtagagac aagtgcaatc ggccaagctc
     8881 cagataaaaa acgtttataa agtagcacag caagaatact cattgtatgt gatggcttct
     8941 ttggaccatt ttttatatcg actgcaatcg gtcctatata tttattattt gtatctttca
     9001 atcgataacc tttttcagta atagcaaaag taactaattg taaactagga ttttcaaagt
     9061 gtcgaatcat cttatcaaag tgttcagaac aatctggatg acaatacaga ctgtcagctg
     9121 tcgcagctaa tatacgtttg acgaactctc cactgcttgg catgaccact tcaagaatca
     9181 aatcatcaaa atcttggtaa gccctttcaa ttatttcttc atcgaatgtt tcacaaacaa
     9241 tgacaccagt ggacaattcg cctagatccg ctaaacgttg agcgatttct gcatgaaatc
     9301 ctcgataaag atttccgcca ccgaaatgga tccagacagg atttttttgt ccattttctc
     9361 ttaatgatgc acggtcaaaa tgaggtattt ctacttcttt ttgagataat tcagggataa
     9421 agttcctcca gttgtttaaa tcaatcattt ttcccctcct aaactaacat attagttttt
     9481 gtattcaagt gaattttatg caataaaaat cgctatgtca accaaaattt tactatttat
     9541 acaaatttac tagttaatgt gacataccag attgtttttc agacctcttc tgttatacta
     9601 cgttcataga aatgattttg gaggaataaa gatgaaaagc agcttgcaat cacaagctta
     9661 tcaagcgatt cgtaaacaaa ttatctatgc tgacttagaa cctggaagga aaatatctga
     9721 aaaggcactg gaggaacatc ttgctatcgg ccggactccc attcgcgaag cattgattca
     9781 actacgtcag caagaactcg tgtatacgat acctcaaagt ggtacgtatg tttccaaaat
     9841 taatcttgtt tctgcagaaa atgctcgctt tgtccgagaa cagttggaag agcaaatcat
     9901 gattgaatgt tgttccaaat tggacacaca aacacgaata gtgctagaaa cgattttaga
     9961 agaacaagaa aaagcagtca aaataaatga cgaacgcgct tttttccgtg cagataattt
    10021 atttcatgaa gcttgtttcg aaattgcagg aagaaaagaa gtttggaact ggttagatga
    10081 ccataatacc catttagaac gtttccgctg gttacgagtg accattcagg ggcttccatg
    10141 ggaaacgatc atggaacaac attatgattt actcaaggct ttagttgata aaaaaccaga
    10201 agaagctcga tttcaaacgg gattgcactt acacatgatg ctgaaagaac aggaaactgt
    10261 tactcaacgt tatcccgagt atttccagca gtagcatttt cttgcgacct cttctatttt
    10321 tataattaac cttcgttttt gagtaatctt tattaaataa caaaatatcc attgactttg
    10381 caactccaac taaattacaa taattataaa taaataatta ttattggagg ttttactatg
    10441 tcattgattg ggaaaaaaat cgaagaattt aatgcacatg cttatcatca aggagaattt
    10501 ttagaagtca gtgaaaaaga tcttatgggg aactggagta ttctttgctt ctatccagct
    10561 gattttactt ttgtttgtcc tactgaatta gaagacttac aagaacaata cagtacattg
    10621 aaatctttag gtgttgaagt cttttcttgc tctaccgata cccactttac tcacaaagca
    10681 tggcatgata catcagatgc tatcggtaaa atcgaatacg tgatgatcgg tgatccgtct
    10741 catcagattt ctcgtatttt tgatgttttg gatgaagagc aaggattagc tcagcgagga
    10801 acatttatta ttgatccaga cggaatcgtt caagctatgg aaatcaatgc agatggaatc
    10861 ggtcgtgacg ctagtgcact gattgataag attcgcgctg ctcaatatgt acgtacccac
    10921 ccaggtgaag tttgtccggc aaaatggaaa gaaagtggcg aaacattgaa accaagtttt
    10981 gatcttgttg gaaagattta ggtggtggat atgttagaca aagaaactaa acaaaatctc
    11041 gaacaatatt tagcactgat tgaatctcct attgtttttt ctgttagttt ggataatagt
    11101 gaaaatagtc aaaaactagc tgaatttacg aaggaaatag cagaaatgtc tcctaaaatc
    11161 tcttgggaaa aaagagattc gatgcgcaca cctagttttt ctatcaatcc agttggcaaa
    11221 gaaagtggaa tcgtttttgc tggtatcccc ctcggtcatg aattttcctc ttttttactg
    11281 gctatgttac aaattagtgg acgggcaccg aaaatcagtg aatctttgag tcttaaaatc
    11341 aaaaaaataa cacaaacatt gcattttgac agttatgtta gcttgacttg tcataattgc
    11401 cccgacgttg tacaggcatt gaatatatta gctgtattaa atccaaatat tactcatacg
    11461 atgattgaag gtgggatgtt ccaaaaagaa atcgatgata aaaaaatcat ggcagtgcca
    11521 actgtgtttt taaatggaga agaatttagt agcggacgca tgacgatcga acaaattgtt
    11581 gaaaaaatca ctggcccttt gattgaagaa gagttgtttg aaaaagaacc ttatgatgta
    11641 ttagtgattg gcggtggacc agcagctagt agtgcagcta tctatgcagc acgtaaagga
    11701 atccgtacag gacttgtcgc tgattctttc ggcggacaag tcgttgaaac actagggatt
    11761 gaaaatatga ttggtacctc ttacacagaa ggtccaaaat tgatgcagca agtagaaagt
    11821 catgtgcgta gttaccctat cgacattatg atgaatcaac aggccgtttc cttaaataaa
    11881 gagcaacatt ttattaatat tggcttagct aacggtatat cacttaaagc aaaaaccgcc
    11941 atcatcgcga caggtgcgca ctggcgttct atcaatgtac caggcgaaaa agagtttaaa
    12001 aataaaggaa ttgcttattg cccacactgt gacggccctt tatttaaagg aaaagagatt
    12061 gctgttatcg gtggtggtaa ttctggtgtt gaagcagcaa tcgatttggc tgggttagca
    12121 aaacatgtat atgttctaga attcttacca gaattaaaag cagatcaaat cttacaggat
    12181 aaactagtct ctcttgcgaa tgtcactgtt atcaaacatg cagcgacgaa agaaatcagc
    12241 ggtacgaatc aggtagaatc tctctcctat atcgatcgcc gtacgatgga gatgcataca
    12301 ctagctgttt caggagtatt cattctagtc ggcttactcc ctaatacgga ttggctagat
    12361 agaacgatcg aaatgaatcc aagaaatgaa atagtgaccg agaaaaatgg cgcaacgaat
    12421 atccccggcg ttttcgcagc tggtgattgt acagatagtc catacaaaca aatcattgtt
    12481 tcaatgggtt caggagctac tgcagcttta ggagcattcg attatctgat gcgcacttga
    12541 gctatgaaga ttgtaaaaag tcagagagcg gaaaaaagga tggtgacaag aattttgtca
    12601 caatcctttt tgtttttttg aataatcgct ttttcttctc tttttcttat atactataag
    12661 tagtaaataa tttgaaaaaa agaaaggtga ctgattatgg cgcaaaatcg tatttttacg
    12721 acaagttttg caagcgttta tcctatgtat gtagcaaaag cggagagaaa agatcgtata
    12781 aaagaggaag tagatgaaat catttgttgg ctgacgggtt attcccaaac agaactagaa
    12841 tatcaactac aaaaacaagt gactttcgaa caattttttg cggaagcccc tgtccccaat
    12901 ccttcacgtt ccttgattac aggtgttgtt tgtggtgtac gagtagaaga catcgaggat
    12961 aacttgatga aagaaattcg ttatttagat aaaatgatcg atgagctagc aaaaggcaaa
    13021 aaaatggaaa agatcctcag aagttgattt ttccagagat attcttctcc ttcgctcatt
    13081 ttgtaacatt taatttggta aaatgactag ttcacgttaa aataagtcaa aggaggtttt
    13141 cttatatgtt tgagacgatt aaacaaaata ttcaaaaata cgccttatta agagctgctg
    13201 tttatattat ttctgggatt gcgattgtgc taaacccttc tgctgttttt cattttatcg
    13261 gttatctgat caccgcctat cttgttttgc ttgggttgtt gaatcttttt gaggcttacc
    13321 gttttaaaag gaaaacaggt tcttggagta tcggtctgtt cagtggtctt tttttcctga
    13381 tcttagctct gatcgtctat gcttttgcac ctgcaatcgt ttctatcctg ccgattcttc
    13441 ttggattagc tgttatcatc aacagcttgt tccagttgtt cttcagcatg aatacaaaag
    13501 ctaaaagttg gagtgtttat agtagtattc tgttgttgat tggcggattc gtcttattgt
    13561 tcaatccatt caaaagtttg atgcttctat tccaaatatt cggctttatt ttgattttca
    13621 tgggcatcca ggagattatc aatttcatca gaataagaaa aatgactata cgttaatcat
    13681 ttttagttat taaaatagac cgtataaaaa ccaacaatcc aaaaaaatct tttggattgt
    13741 tggttttttt gtgagtatat tttcctattt tttcaataat gtatgttatg ctaagttcgg
    13801 gctatatttt atacaaagga atgatgagaa tggaatttat tggtatttta tgcctgatat
    13861 tgatttcaac aacgattggc tcacatatct cgcggagatt aggtatccca gcagtgattg
    13921 gacaattact tgttggtgtt ttgctaggcc aagctggatt gaattgggtc aaacctgata
    13981 ttctagtcca tgacttttcg gagattggtg tgatcttatt aatgttttta gctggtctgg
    14041 aaagtgattt atctcttttg aaaaaatatt tccgacctgg tatgttcgtt gctttgcttg
    14101 ggatcatctt cccggttgtt tttggctggt tgacaggcaa agcattccaa gtttcgggaa
    14161 atgaagcgat tttctttggg attattcttg cggcaacttc tgtcagtatt tcagtagaag
    14221 tattaaaaga actaaacgta gtgaatacaa aagaaggatc aacaattttg ggtgcttctg
    14281 tggtagatga cattttagtc gttttagttc taagtttcag cctatctttt ctaacggggg
    14341 aagcttctag caatgcttct ttaccgcttg ttttgattga acagctaatt tattttgttt
    14401 ttattttctt attagtcaaa tggattgcgc ctttcctact ttcatttgca gaaaaagtgt
    14461 acgcaaattc ttctattatc atcgtatcgc tagttatttg tttaggaatg tcttacgtag
    14521 cggatcttgt tgggcttagc tctgtcattg gtgctttttt tgctgggata gctgtcagtc
    14581 agacaaaggt taaagaagaa gtataccaca atgtagaggc tttaggatat gcagtattta
    14641 taccagtatt ttttgtcagt gtaggattag aagttgattt ttctgacttt agtgaacaac
    14701 tactctttat ttctatattg acgcttgtag cgattttgac caaattgctt ggaggttatt
    14761 ttggtgctca gattgccggt ttttctcaac atagttcatt gatggtcggt gctggtatga
    14821 tttcgcgtgg agaaatggct ttgatcattt tacaaattgg acagcaaagt caattgatcg
    14881 aaccacagta ctattcacct ttagttatcg tgattttatt aagtacactt atttctccac
    14941 ttgttctcaa atattttaca aaaaaagttt atgaagtgtg agtaaatcaa aaagaagttg
    15001 tgaaagagac atcgtgaaaa ccgtttattt gaaaaaagga ctgtgacgat aacgccacag
    15061 tccttttact gtacttaaaa agcaattttt tattgataat tttggttgag gtattgcaat
    15121 agttccgatg ctcgtttggt tgctgaatca tcatcttctt tattttccaa catgctgacc
    15181 atcatataac cttggttatc aagattgaaa gcaaacaaga aactgttctc ttgccccttt
    15241 acatcctgtt tttctttgat ttccgctgta ccagttttcg ctgccaacgg aatccctaaa
    15301 gcagaaagag aatgtgctgt accatttaca tcttgcacaa cttctctcag atctggcacg
    15361 atcgtttgta ctgctgtttc gctgatgaca ttcttcttat cttttgtctc tttatctgca
    15421 atcaatttag gatagacaag tgtgccattg ttggtaaaaa cagaatacat tgctgcttgc
    15481 tggataggag taattagaag ttcgccctgt ccatatccag tatcagctag caagatatct
    15541 gagttaaagc tatcttcatt agaaatttgt gctggattca tactaattgg caaatcaagg
    15601 tcttcaccaa aaatgaattt atctaagcct gtacgaaatt ttttctcacc cattttcaac
    15661 gtttcttgtg ccgcatatat attatcggaa tagatcaaag cagtttttaa gtctacttgt
    15721 gatacatcac tactgacacg cgttgtttta aaagatcccc aagaactgtc tttttgccat
    15781 ttaagcccgt tgatcgtcaa cacttcattt ggatcgatag tgccgttgtc gagaccgatt
    15841 gctgctgtaa tcattttaaa cgtagatcca ggagcataac ctgtcgcaaa tcggctgatg
    15901 aatggatgtt caggattttc ttcataagct ttgtaatctt cttgtgagat cccgtttgtc
    15961 attttgtttg gatcatagct tggagagcta gcaagcgcaa gaagatcacc ggtttttggc
    16021 gttgtcgcaa cagttgatgc agcttttcct cctagactgt caaaagctat tttttgtgcc
    16081 ttcgcatcga ttgtcaattt gatatctttg ccattttgaa cttcatgttc gatcagaact
    16141 tttttctcta caccgtctgc atctgtaatg cttaatttcc cacctgttgt cccacgaaga
    16201 tccttatcaa aagccatctc caaacctgag cgaccgattt ttccattact gcttaattct
    16261 ggattcttat caatatcttc tgctgtgata tctcccacgt aaccaatcag ctgagcagct
    16321 gcttcaccca aaggataata tctgccgtct acttcttgaa tggtagctcc agctggaagt
    16381 tctggcgttg ctccatcaat gattttcaat gggacaaagt aatcgggttg tacccagctt
    16441 tgtgaaatcg cctgattgat agcatcttct gttaagtcga aggaggaagc aatcgctttg
    16501 atattggctg ttttttcgcc tccatctcca agtttacttg gaacgactcc taattgtttt
    16561 agtttgcctg ttgttgctaa tggttcacca tttcgatcta taatattccc tcttgctgct
    16621 tcttgtgtgg tcaggcttac tttgtcattt ccttccattt ctggaaaaac caagttgggt
    16681 tgccagttga tcgtggtttg accatcattt ctgtctaatg ttcctttata agaaagatct
    16741 ttcaattcac ctaatgaggt attcatcttt gctttatatg aaaaactgta agtagaatcg
    16801 tcttttttat ctacttttag attcgatatc tgaagtcctt tgacatcagc agcaccgtat
    16861 atattctggt atttatctaa gatctctttt tcagataatg cacttttatt tgccgccttt
    16921 tttgatgtca tttctgcagc tttgttataa tctcccttgt tcaaagcttg gacaaattgc
    16981 tcaactgtct tttctccagc ttctactgct tgggtttctt gatagtgctg gtagtaaaaa
    17041 taaccgccac ccgcagctgc tattaagatc actgcgccgg caatataagc gcctgttcga
    17101 tttttgccgt gcttgtcact tcttttcatt ttttcctcct tctcttgctt gtcattcctt
    17161 tatttcacta tttatacctg tttattctaa catatgtctt tgaggaaatc cataccacaa
    17221 tccagaagca cactttctgg aaggttcgtt tggatactac caagtaattt cccaaccgct
    17281 ttgggtagct tgaatattga agttacgtct ttcagctgct gagagatagc tttgatcact
    17341 tgctgctgcc ttctgatcct cccgaagtct ccttctttgt cttcccgaaa tcgagcatac
    17401 tggagtaaag tattaccatc catcacttgc tctcctttgt ttatatatac gccatctaaa
    17461 tctagagatt tttctgcatc gatcttgact ccaccaggaa acagttcatt aacgcaatcg
    17521 ataaaatctt gaaatgtgat acttgcataa tagggagctt caaatttgaa attttccttt
    17581 aatgtttgat ttaataaatc aactccccca taagagtaag ctgcatttat cttattcatt
    17641 ccgtaacctg gaatatcgac atatgaatct ctcatgatag agatcagttt tgcttgttgt
    17701 gttttctggt tgtattgtgc aaccagaagt gaatccgagc gacctccacc aatcgatctt
    17761 ttttcaccgc atctcctcct cacacagcaa tccacttgtc aggtgaggag cgagtttgtc
    17821 tcatcaaata agtcagtcac aaaaatataa agaaaaggtc agaaaccttt tcgaacaaga
    17881 attctgacct agcatcatct catcgtccga gttttagcac tatacaacgt aaagaaaaca
    17941 ttcttagcta ttggaaaaag ccttaatcca acagtttctg ttttacccat tggcgacttt
    18001 cgacgtttct gggcaacagc ctatgtttgt ataggagcct cacctaacaa ggttattact
    18061 atattctatt tacgagttca tgatagactt ttcctaatga ttaggcaatg gaatttgaaa
    18121 tgaaatagtg aaatttaaca atctttaata ttttttagaa aaaaataaca aataagttga
    18181 aagaatcccg ttgcaaattc tttcagttgc accaggattc tttgataatt ttttagtttt
    18241 atgtgttttt ttatatgttt tttcactttt cggaataaat atttatttgt taaagtaatt
    18301 aaacaatccg ctaatcgttt gatctggcat ttctatttcc ttaccatcta aagtaacagt
    18361 agtaacgaca aaatggtctt gcaatggcgg aacttctaag tactgccctt gcagaaaatc
    18421 attcactgtt tcatattctt ttttcatttc ttttgaatct gtcgatggtt tatagcttat
    18481 ttttaacata actagtgctc ctttttattc gggacgtgca atcgtgaaaa tttctcctat
    18541 tgctgcataa tcatttcctg ccaaacggct tacagcattc aattttcttg gatcgatcct
    18601 tcctgtctta tcatcataga ctgtttcatt taaatgatat ttctgtactt ctagcaaaaa
    18661 taaatcagct gttactttat ttttattccg tatctcgaca tgttcatata aggatgtttc
    18721 aaaacgaact tttgcttctg tcaatgctgg tactgaaatg attactgaat cagttaaagt
    18781 caagtcagtc aggtacagtt cgctttcctc attaggcaga ttggctgctg ttttatttac
    18841 ttcttctacg ttgtcctcat caaggatatg gataacagct tctcttgtct caagaatatt
    18901 ctttgctgtg tcttttactg catcttgttt tctctggatc gccaaggata agatgggtcg
    18961 attcgaactg acgatattga agtaactgaa gggtgcgata ttcactatat cttttgctga
    19021 tttagtcgtt actactgcta ctggtctagg gatgactgaa ccaatcagca acttgtaatt
    19081 ttcacgttct gtcaattctg aaggactccg ttcaaacatg gtttcgcctt ctttctataa
    19141 aattcgttaa aaagcagatc ttgaagacaa aatgaaaagt aatgcttttt tcatcttctt
    19201 agaatctgct ttaaactatc tatcaatttt tcttttttgt caagaatctg tgccgtgcgt
    19261 gttttttagt tatttgcttt acttgcggct tcgacatatc ttttagtgat caattgcgga
    19321 cgggtgataa tagatccaac tacgactgaa aaagcaccta attcatatgc tttctctact
    19381 tgttcaggag agtcagtatg tccttccata attactggga tatcgacttg ttctatgatt
    19441 tgattcaata aatcaaaatc aggtgtctgc gtctttcgtg aatattctgt atagcctgct
    19501 aaagtcgtag acaaaagatc aaaaccatct tcttgcgctt gaagcgcttc ctcaaaagtc
    19561 gaaatatctg ccatagctaa ccgaccgtgt tcgtgaatca tttggacaag ttcagcagtt
    19621 gttttttctg tcggacgttt acgagaagtt gcatccagtg caatgatttc tgcttgcgtc
    19681 tccaaaagtt cttccacttc tttttctgtc gcagtaataa acacttctga cccatcgtaa
    19741 tctcttttga taatccctat taccggtaaa tcaacgattc gcttgatttc attgatatct
    19801 tctgtcgtat ttgctcgaat accaactgct ccgccttctt ttgcagccaa agccattctc
    19861 cccataataa aagaactgtg tagcggctcg ttttccaaag cttgacaaga aacgacgact
    19921 ccgcctttga tttgttccag tgtatccaca atatctcctc cagtaatgtt atataaaaat
    19981 acagcttcat ataaaaatgt aacccttttc tattcaggag acaataatga gctatttctt
    20041 tcagcagacc tttcttacta aaagaaaatt acttcaaatt cttcgtatac gaccagcttc
    20101 tcttctgtaa attctatgtt caacgcttct ttataagcat gtttaaaaaa ctcagcatgg
    20161 actttccgcc aataagctaa tgtccgatcc ccttctcctt ctttccaagc atgctctttc
    20221 gtgacttcac taaaaggcaa gatggtcaca tttatatttt tgatgataca aactggttca
    20281 ttttttccat tcaggaccac gtctaattga ccaacttttg gtaaaggctc attctcttgt
    20341 ttatacagat cgtaaccact tgatgtacca gtctttatct tttttacaac tagttcgctc
    20401 aactcgtctg ccattttttc tgtatcccca aatgaaaaga aacctaattt ttctgattga
    20461 taactaggat atttttcttg gaactttttg acatattctt ctacttgctg atacataaca
    20521 tccacccctt tgctttttat aatgaaaaaa ccatccagat actagtataa acaattatct
    20581 ggatggtgag cataatgaat tattgaatat tcaagacacg cagactatct tcaacgattt
    20641 gttttggtgt taaatgataa cgttcataaa gctgccctac aggaacgcta tccgtgaatt
    20701 ctttctctgc accaaaattt aatacgttca ttgaagaatc accataataa cgactgatat
    20761 tttcgccaaa tcctcctgac aaactaccat cttctaatgt gatgactaat tcgtgtgttt
    20821 cttttaattt atctaatgtt tctacgtcat tttctgtgat cgttcctgga ttgatcaacg
    20881 ttgcttcgat tccttttttt ctaaaagctt ttactgtttc ttttcctaat tcaagaaaac
    20941 cgcctaaagc taaaattgct acttttcctc cattatttac tgtttgatag gctggaatac
    21001 tataatcatt tgcgatagct gtaccatgtt ctacaccatg tgtaggaatt cggattgcta
    21061 caggctgcgc tgtttgttcg aacgcccaat ctagcataga gatcatatcc tcttttgttg
    21121 ctggagcaag atatttgatg ttcgggatac ttgataagta aggaatgtca aatgttcctt
    21181 ggtgtgttcg gtcactagct gagatcgttc cgccaccgac taaaataatc actggattct
    21241 cgttgatcgc taaatcatgt gataattgat catatgcccg ttgcaagaaa gtacttgatt
    21301 ggaagacgac tggacgtact ccctgagaag ccaaagcaga agcgaaagtg atagagtgct
    21361 gttcagcgat ccccacgtca aaataatgat ctggatgttt ttcttggaat gttttcagtc
    21421 caaaggcgcc tggtatcgct gcattgactg cggcaatagg agctgtactt gtttgcattt
    21481 tcttatccaa ataatccatg atgatcatat taaatgtttc tccagaagtt acttgctttg
    21541 cctccccggt ggacagatca aatggtacat gccaatgata tttcatttta ttatcgattg
    21601 ccggctgata accgcgacct ttttccgtat ggacgtgaag tacaatcgga tgatcaatat
    21661 cttttacttc ttcaaataaa cggatcaccg tttccagatc gtttccatta tccacgtaac
    21721 gataatcaaa tcccattgct ttaaataggt tattgctgct tttaccgttg gtttgacgaa
    21781 gctcagctaa gtttttatat aaactgccat gattctcatc aatagacatc tcattatcgt
    21841 ttactaatac gattagattg gattgtaaaa caccaccatt gttcaatcct tcaaaagcta
    21901 agccaccact tagggagccg tcaccgataa atgctaccac atttcctgat tctttcttca
    21961 gatctctcgc tttagctaaa cctgttgcta agctgataga tgtagatgtg tgccccactg
    22021 taaaatagtc atgtttactt tcatgttggt tggaatatcc gctcacttca cggtaatgtc
    22081 ctttttcaaa accatttttt cgaccagtta aaagcttatg accataagac tgatgggaaa
    22141 catcccaaac gattttatcc tttggtgaat caaatacata atgaaacgca atcgttaatt
    22201 ctactacacc tagattcgga ccaacgtgcc cacctacatc gctgtccttt tgcaagattt
    22261 tttggcgcat ctcttctgcc aactgtttca tttcctcaac agataatttt ttgacatctg
    22321 cggggctatt gattgcatcc aatacatgta atactgtact cataatacat tctctcctcc
    22381 gtactatctt cttttaatga aaatagattt gttacacttt atctattcta ctacttagag
    22441 ttgactctaa gtcaatagca ttttaaaaaa atttcagaga aagcttgtta tataagtaga
    22501 tcttcgcacg aatcaggact caaataaaaa ccttctacag acaattaaaa agctttgtag
    22561 aaggtctcct aattaaggtc tcatcaagaa atatgttttt ttaataggtg aagttcctga
    22621 gcaatatgat acaaatggat gtttgcacct tttaatgaga ttggatagat ctttgtcttt
    22681 tcagtttcta atccattttg atagctagca gcatcgcaca aaaccactct ttttttcgca
    22741 catagacatt catcaggcaa ttcttctaat gaacgaaata aaataggatt tatttcagcg
    22801 atgccttctt ccgcatacaa atgaaattgg gttcgataaa aaaattgttt gactgaactt
    22861 tctgaaacaa caaaaatttc tatttcctgt ctccccgctt gttcactgtt gatcaaagaa
    22921 agacttttga cgaattttcg aatgaaattt tcattccatc ttagttggat ttgatttttc
    22981 tttttccact cttccaatat ttctacaatt tttccgtaca gaagttgttc tttttctgat
    23041 agaagataga tcctttccgg taaaaatact tgtccatctg cccacaacgt acggacaaat
    23101 tgtaaaaagg attgcttgaa aataaaattt tctgcatcgt cgatttcagc gatactaata
    23161 atctgtaaaa tcaattcatt cagtgatagg tcattgagaa caaaattttt ataaacaact
    23221 tcgaaatctt ttcttaacag ctccatgttc tcattggtat aatttctact gttgaacaaa
    23281 gaaaaaatat aatagacatc ttcttcttgt aagtctagaa ttcctttttg ctcttccaat
    23341 ccttgacaga ctaattgaaa taacggcttc gtttctgcgc ttttcttatc ttcttctttc
    23401 agtccaaggg gaaatgcctt tcttccgatt gcgatctcta ttcctcgtat gataaaggac
    23461 ttttcatctt ccgaatatct tcttttaaaa aaatgagtct ctacataatc aatcaactcg
    23521 ttacaagctt cttctacata cggattttta ttttcatagc cttcccaatt gacataacgt
    23581 actaaagcta gcaagatgtt ccgcgagtca aattctggga taatgatttc tttatttttc
    23641 aaataatcat tttctttaaa aaaatccaat acgatttttt tgattgtata tactttgctc
    23701 aaagaaataa attcaagatc agccagttct gtactcgtga atataccatt aaaataataa
    23761 cttaaaaact ttaaaaaata agattgaccg tacagaaact gtgtgagctc agaaagagca
    23821 ataccatgtt tgaatacgag tttatactta tgcttctctc ttacttgttc ttttatacaa
    23881 tattctagac actcggctag actttctaga tcgtgaagga tcgttggtgt agtcaccctt
    23941 agagaaatag ctgtttgttc gatgtctatt tcttggaatt caaataattg ttcacacaga
    24001 tacacttttc taaatatatt tcgctcgatg taattttcca gcattcctga tacacgcctc
    24061 cagtttttct tatgatttta tttaatcata tctgtaaatc gtatttttgt ttccttttta
    24121 tttagaaaag ccctttcaaa aaccaataaa aaaagaaact gggacaaaag tcaaacaagc
    24181 tcacaatttc agcctcattg taggtagctt tgagccgttt ggacttttac cccagcctct
    24241 ttcaagaatg gttattcaaa gatgtcagcg tctagttctt tgactacatc tttcaacaat
    24301 tggataccag ctgcgctgtt accaaatgga tctagtgccg ggctgaagac acctatgccg
    24361 taacgatttg gcacagctgc catcaacccg ccgcctacac cgctttttgc aggtacacca
    24421 acatggacgg aaaactctcc tgattcatca tacaaaccag cagttgtcat aattgatttg
    24481 acgattgtag cactttcttc agtaatgatt tgtttgccat tccaaggagc aattccttta
    24541 tttgccagca cagcagctaa ttttgctatg cctgtagcgg tgacattgag ggagcattgt
    24601 ctgaaataag catcgagtat ctccggtacg tctccttcga tcatttgatt acctttcata
    24661 tagtaagcaa gagagcgatt gatatcacct gttcttgatt cagaatgata gatttcttca
    24721 ttcaatgtga tattaggatc atcacagatc tctttcataa atgacaagat tcgctcaaat
    24781 ttttcatctg catctttacc ggcaatcaac gaggttgttg caatcgctcc tgcattaaca
    24841 aaaggattca ttggatgttt gcgatgattg atttccatat tcatgataga attgaaggca
    24901 aaaccggttg gttccgtgtt gattgtttga aagacttttt ctattccgtt gtctttgatt
    24961 gctaataaca atacgggaac ttttgacatg ctttcgatgg caaagcgcac atcggcgtct
    25021 cctccctcaa tcagttgttt tgtatccaaa tcataaatcg ccatacctaa ttgtttggga
    25081 tccacttcag ctaatgctgg aatgtaatcc gccacttgtc ctgtttgata atagtggaaa
    25141 ttttttccaa tcaattgttc taattctttt acttccattt gaataaccag ctttcatttt
    25201 ttatttttct gcatgctgga atttatgacg gcatgcgtag atcgtaaatg gaataactaa
    25261 gacgataaga taagaaacaa tcagaatcgt ttcataaatc ccatgctgtc cagctgcgat
    25321 actagcaggt ggaacaaatg agatacaaaa agcaaaaagt gaagtcaaaa gacctaaaca
    25381 agcaacgatt acttttacaa cttttccgcc tggaacctga taggctcttt caagactgtc
    25441 atctttaaaa accagcttca aatagcccgc gaaaaacata aagtacaaca tcaggtagat
    25501 tacaacagtc aatgacatcg ctgcaagaaa tgaaagatta ttccccccgc cgcttaatgt
    25561 aaagacggct gcccagattg ttgcaacgat cccatttgcg atcatcaaat gagcaggaac
    25621 accgtgtgtg ttttcttttg caaaatattc aggaagtaat ccttcttctg ctgctacgtg
    25681 aagtgctttt gaaggagagg ttacccaaga agcaatttct ccgatgacac ctaaacaaac
    25741 aagaatcgca agaattttcg caatccatgc aagatttgga tcaatgtgtc ttaataggaa
    25801 ttctaacgat tgaatcaccc ctgtattaag agacaaacca tgttgaggta ctgtagtagc
    25861 gacggtcaat ccgccaaaag tatctaaaat gattgccaga ataaccaata caaacattgc
    25921 taatggataa tcctttttag ggttttccat ttcatttgca tgactggcag atgtttctac
    25981 gcccatatat gctaaaataa aggaaacaaa aacaaccagc gtaggcaatt tcgaaaaatc
    26041 aggaatcaat gttttcatag acagtatcgt ttgtagtgga gcgcctgaag cgacataatg
    26101 gatccctaag ccaaacaaga ctaatgctgg caacaagata ccagctacaa aaccgatccg
    26161 agcaatcaat gaagtgtatt tggttccttt taactgtgag agtaaaatag cccagaaaat
    26221 aatcaatagg attgccaatt tcagatattt attgttttgt atagcaccaa tacccgttgc
    26281 ataggataat gcaccagtta agaaatacag catggtcaag tagccaactg ttatctcaaa
    26341 ccattggaaa aagattgcgg caaaaccgaa tcgttttccc aatgtgcgac tgacccaagt
    26401 atagacaccg cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac
    26461 agggataaac catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt
    26521 cgcgaatgtg ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc
    26581 aaagaacgtt aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct
    26641 tttctatttg aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc
    26701 ttcttgttct tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac
    26761 tgttgtcagc acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa
    26821 gaagtaccag ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag
    26881 aatcgtacct gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca
    26941 ttgtttcttt ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat
    27001 cagcatggca ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc
    27061 aacggatcct gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat
    27121 cagccaagta atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc
    27181 ttgttcatct gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag
    27241 tcctgtaccg atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat
    27301 ctttctttga ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat
    27361 gactgctcca ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat
    27421 ccagtggcaa acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc
    27481 ttttaaattc aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa
    27541 catcagttgg taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg
    27601 gaagcttgta acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat
    27661 atcccgtcca aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac
    27721 ggagagaaaa gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta
    27781 aaaaaaagct agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc
    27841 tgtatctcct gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg
    27901 agtataaaag aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt
    27961 ataaaagaca gacaattaat aaaagcttga tgtgttgt
//
LOCUS       DAJSBM010000028        27750 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJSBM010000028 DAJSBM010000000
VERSION     DAJSBM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.028"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            320..2086
                     /locus_tag="ONT67_001475"
     CDS             320..2086
                     /locus_tag="ONT67_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCR2355222.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2188..3447
                     /locus_tag="ONT67_001476"
     CDS             2188..3447
                     /locus_tag="ONT67_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2355223.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3594..4895
                     /locus_tag="ONT67_001477"
     CDS             3594..4895
                     /locus_tag="ONT67_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355224.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4898..5749
                     /locus_tag="ONT67_001478"
     CDS             4898..5749
                     /locus_tag="ONT67_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355225.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5762..6577
                     /locus_tag="ONT67_001479"
     CDS             5762..6577
                     /locus_tag="ONT67_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCR2355226.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6728..7894
                     /locus_tag="ONT67_001480"
     CDS             6728..7894
                     /locus_tag="ONT67_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2355227.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8124..8771
                     /locus_tag="ONT67_001481"
     CDS             8124..8771
                     /locus_tag="ONT67_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2355228.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8855..9799
                     /locus_tag="ONT67_001482"
     CDS             8855..9799
                     /locus_tag="ONT67_001482"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCR2355229.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9934..11385
                     /locus_tag="ONT67_001483"
     CDS             9934..11385
                     /locus_tag="ONT67_001483"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCR2355230.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11406..12242
                     /locus_tag="ONT67_001484"
     CDS             11406..12242
                     /locus_tag="ONT67_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2355231.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12242..12901
                     /locus_tag="ONT67_001485"
     CDS             12242..12901
                     /locus_tag="ONT67_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2355232.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            13027..13716
                     /locus_tag="ONT67_001486"
     CDS             13027..13716
                     /locus_tag="ONT67_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355233.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13722..14678
                     /locus_tag="ONT67_001487"
     CDS             13722..14678
                     /locus_tag="ONT67_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCR2355234.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14722..15234
                     /locus_tag="ONT67_001488"
     CDS             14722..15234
                     /locus_tag="ONT67_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355235.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15490..16038)
                     /locus_tag="ONT67_001489"
     CDS             complement(15490..16038)
                     /locus_tag="ONT67_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2355236.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16127..17308)
                     /locus_tag="ONT67_001490"
     CDS             complement(16127..17308)
                     /locus_tag="ONT67_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR2355237.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17544..19052)
                     /locus_tag="ONT67_001491"
     CDS             complement(17544..19052)
                     /locus_tag="ONT67_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCR2355238.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19211..19402
                     /locus_tag="ONT67_001492"
     CDS             19211..19402
                     /locus_tag="ONT67_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355239.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19605..20522
                     /locus_tag="ONT67_001493"
     CDS             19605..20522
                     /locus_tag="ONT67_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCR2355240.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20515..21588
                     /locus_tag="ONT67_001494"
     CDS             20515..21588
                     /locus_tag="ONT67_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355241.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21736..22254
                     /locus_tag="ONT67_001495"
     CDS             21736..22254
                     /locus_tag="ONT67_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355242.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22235..22627
                     /locus_tag="ONT67_001496"
     CDS             22235..22627
                     /locus_tag="ONT67_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCR2355243.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22703..23638
                     /locus_tag="ONT67_001497"
     CDS             22703..23638
                     /locus_tag="ONT67_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCR2355244.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23771..24550
                     /locus_tag="ONT67_001498"
     CDS             23771..24550
                     /locus_tag="ONT67_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCR2355245.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24543..24860
                     /locus_tag="ONT67_001499"
     CDS             24543..24860
                     /locus_tag="ONT67_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCR2355246.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25104..26714
                     /locus_tag="ONT67_001500"
     CDS             25104..26714
                     /locus_tag="ONT67_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2355247.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26871..27719
                     /locus_tag="ONT67_001501"
     CDS             26871..27719
                     /locus_tag="ONT67_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2355248.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8996 a         4902 c         5557 g         8295 t
ORIGIN      
        1 tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt ctagaatata
       61 cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa attgatcgct
      121 taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt aaaagtttta
      181 agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac tgttaccgat
      241 aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga cgtcatatta
      301 aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg aaagtgaata
      361 tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga aaaatgatat
      421 caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa aatggtatca
      481 aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt gggaaattga
      541 agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg aagataacac
      601 ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga ttactgaacc
      661 aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta ttccagaatg
      721 ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg gggacaaagc
      781 aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag aagacttttt
      841 cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc ttggcatcaa
      901 tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg atacgattga
      961 ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact tgattgatga
     1021 agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata tgggtgtcca
     1081 ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata aagactggtt
     1141 ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag aaactgctga
     1201 aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa atacagcaaa
     1261 tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag aattcgatat
     1321 tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga aaaaattcag
     1381 agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt ggcattcctc
     1441 tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat ttacagattc
     1501 gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca gtggaatgaa
     1561 tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact tattagattc
     1621 tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga tgaagtctgt
     1681 gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga cagaaatagg
     1741 gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga aggaggaaca
     1801 agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac agttgtctaa
     1861 gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag ataagctcta
     1921 tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt caaaagaacc
     1981 ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt tgttagaaga
     2041 cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac tgttaccggt
     2101 aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg gtgtaacagc
     2161 acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca gttgggttta
     2221 gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg taactcttca
     2281 gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc tggatataaa
     2341 gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa aatcaagcta
     2401 gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc tgctggaaag
     2461 ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca gcaaggacat
     2521 ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa agcccaagtg
     2581 acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt tttgtactat
     2641 aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa tctatccaaa
     2701 gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt agctaaatgg
     2761 actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt atttggtgat
     2821 gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga aggaatctct
     2881 tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat aaaaagtgca
     2941 ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat tgacggtcca
     3001 tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa aattcctact
     3061 ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt agtcagcaat
     3121 tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa tcaggaaaat
     3181 caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc tagggaaaca
     3241 gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga aacagctcaa
     3301 gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa cttgatgttc
     3361 gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt aaaaacaatc
     3421 agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt tataaaaaat
     3481 ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct tgctcttgtt
     3541 gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt cagatgcagc
     3601 aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt attccaggga
     3661 tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc atattcgtcc
     3721 tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg gttactttgg
     3781 gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact ttgcaattca
     3841 tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct tatcgagtag
     3901 cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc atacataata
     3961 taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc atgattttta
     4021 cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat gacttcaatc
     4081 atattccacc agccagttta attgattggg taggggctaa aaactttttt agtatcttct
     4141 ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg atttggactt
     4201 tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt gctcaccaga
     4261 aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg gcggtcccag
     4321 cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt gccatcaata
     4381 ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca atctcttgga
     4441 aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg ttaggatttc
     4501 cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac ttatatgaag
     4561 cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg cttcctgctg
     4621 tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc aataatttct
     4681 ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga gctggttcta
     4741 ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg cagtattcta
     4801 tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca ttgctggtat
     4861 ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc attcgagtgc
     4921 tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa tactgtcagt
     4981 cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag gaaatattgc
     5041 agcttttagt ttagatcttt caagtgactg gactttagga aactttactc ggctattcag
     5101 tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc taacaatgat
     5161 cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc gttttatcgg
     5221 ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta tggcagcatt
     5281 gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact ggttcttgac
     5341 aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag gatactttga
     5401 cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc atttcaggat
     5461 ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag cattatgggc
     5521 ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa cacctgaaaa
     5581 tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc aaagagtctc
     5641 tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat tcttcttttt
     5701 acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg agcagagtat
     5761 catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc aagtgtttgc
     5821 gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt tagtttcctt
     5881 gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc cattagaaca
     5941 atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt ctgaacttga
     6001 attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc ctgaaatttt
     6061 ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta ttgattttga
     6121 gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct atacgatgaa
     6181 ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc aacgattttt
     6241 agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata gtttaagtct
     6301 tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct ttttcttgtg
     6361 gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg caaatttgat
     6421 gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta tccactttga
     6481 ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt tatgggtttt
     6541 tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga tttatgcgac
     6601 aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga aaaaattaaa
     6661 cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt ttttaaggag
     6721 gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt tctcgctccc
     6781 tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg acactcctcc
     6841 ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa gtaacgcttc
     6901 tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa gtatccaagc
     6961 attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag attatctaaa
     7021 atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata tccatgcgca
     7081 ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga cttattcttg
     7141 tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata aagtccatca
     7201 tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg gttgtggctg
     7261 gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg ggatcacact
     7321 aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt tagaagataa
     7381 atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg atcatatcac
     7441 aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt tcaaagtggt
     7501 aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc gacaacaagg
     7561 cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg tccctggtgt
     7621 tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt tggaaagctt
     7681 acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc aagtccaaga
     7741 tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat atttacaagc
     7801 ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc ttgttcaacc
     7861 tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact gtttaaagac
     7921 tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg tctaacttct
     7981 aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa agtttggttc
     8041 tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata gaatactatt
     8101 ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt ttatttgcag
     8161 ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg aatacgtctt
     8221 ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca agcacatctt
     8281 cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc aaagtgtctg
     8341 cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt actagtttgg
     8401 aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat gatcaaaaag
     8461 aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat ccagaaactc
     8521 tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat gttgataaat
     8581 tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc ggtacggcaa
     8641 cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa gtaaaagacg
     8701 gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg tcaactgaac
     8761 aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt catgatacac
     8821 tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga cctttgccaa
     8881 tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga ctacccagga
     8941 attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct gtacgatcaa
     9001 tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga gtatcaattc
     9061 tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg cgtatgaaaa
     9121 agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga gtgtccagga
     9181 aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca ctgaattaaa
     9241 tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg aggccactta
     9301 tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca aattgcctcc
     9361 ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt ttccattgac
     9421 ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag aacaagaagc
     9481 agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata tcaagccgac
     9541 tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc aaatcattgg
     9601 tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg gcgcaagcat
     9661 gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta gaatcataaa
     9721 agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat ttaaaggcaa
     9781 attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt ctttttttat
     9841 aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta gacgaacttg
     9901 tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga attagcaaaa
     9961 gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca gaaaaaaata
    10021 aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga gagtaattcc
    10081 cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc atttccttta
    10141 aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg tttatttgaa
    10201 acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat aggtctagtt
    10261 ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga cccagtcatc
    10321 tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc aagtggagga
    10381 gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga tggcggtggt
    10441 tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc gcgagggacg
    10501 atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga tttcggcttg
    10561 acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat ccattccggt
    10621 gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa acggttgaag
    10681 atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc agtacttgct
    10741 gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga gattccttac
    10801 ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc agaaacagca
    10861 gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga agaacttgaa
    10921 cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac ttacatcgat
    10981 tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca agaatacgat
    11041 ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga tttgcaaagt
    11101 gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga aggtttagac
    11161 gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca gttggctaat
    11221 ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa catgcctcta
    11281 ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat cggctatcta
    11341 tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa acaaggagaa
    11401 gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt attttaagcg
    11461 cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta gaaaaaaaga
    11521 aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat aatgatcaaa
    11581 atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg ttaaatgtca
    11641 aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca aaaagagaat
    11701 cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa ttctcccttt
    11761 cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat gattcaggaa
    11821 tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat actagctata
    11881 tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc acgaatgatc
    11941 agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat tattatctgc
    12001 agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag aacgtttatt
    12061 tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg aaaaagaaaa
    12121 tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata tctgaaaaat
    12181 attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg gtgagtgagt
    12241 gatgtacttt cctctaattg atttttccct catgtggact tcacttcctt tcgtactgca
    12301 aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa atctggtagg
    12361 gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat ttattcgttt
    12421 ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt tatattttgg
    12481 gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga ttaccagcag
    12541 tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag gtcagtggga
    12601 cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg ttttgccgca
    12661 agcttttcga atctcagtac cggcactcag taacgtagct atggatctag tgaaaggtac
    12721 ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga tcataggcgg
    12781 tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt gggtcatttg
    12841 tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatgcatc agtcaatctg
    12901 aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt cttgtatatt
    12961 ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg ttcggaggta
    13021 gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca atattgcatc
    13081 gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg aattgtagat
    13141 acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta tgagctgaaa
    13201 gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca ttataattat
    13261 tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac aggaataggt
    13321 gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg tacatttctg
    13381 attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt gacaaaccgc
    13441 ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg aggcgtaaaa
    13501 tattttccaa cagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa tcaagatcca
    13561 ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca ggcaatcaat
    13621 gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag gcaacaacag
    13681 cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat ccttatttaa
    13741 gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga ttgctctcca
    13801 ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa aatgtgatcg
    13861 aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa gtaggacaat
    13921 tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc tctgtcgtcc
    13981 ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct ccagcgtttc
    14041 ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag ttggcaggag
    14101 ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg cccgaaaaca
    14161 aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat gcagataaaa
    14221 cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat attggatttt
    14281 ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc cgagcgcttg
    14341 ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat tttgtctctg
    14401 acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga gataaaatga
    14461 aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg attgaattat
    14521 tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag tggtcactta
    14581 tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg cttaaatatt
    14641 attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta aatgaaaaag
    14701 gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga agtagaagaa
    14761 gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc tcctcaatgg
    14821 caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag aaaagaaagc
    14881 tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc aagcgtggat
    14941 cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta tttatcgatt
    15001 catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc gttcttagaa
    15061 catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga tacttatcca
    15121 gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca agggatgatt
    15181 gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa ttgactataa
    15241 aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca tatgatagcc
    15301 aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc gaaaatgctt
    15361 ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg aaactccctt
    15421 catcgagagg tcgtgacata gcttttgtca cgacctctcg gcttatccta cgagtattat
    15481 tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt ggaacgctcc
    15541 atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact gtgctaaatc
    15601 ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac gttttttcag
    15661 gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta ccgtcccgaa
    15721 atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat ttcctactgt
    15781 cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat attggtccca
    15841 ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa caaaataata
    15901 atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa tgatcttttg
    15961 ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag gaccgataaa
    16021 tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga aaaagctttg
    16081 gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtatattat accagcaagc
    16141 cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt ttcaatccgt
    16201 gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca gaaatgatcc
    16261 cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct gttgtacgct
    16321 cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca tttgtaacca
    16381 gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt ccgctgatga
    16441 aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg aatatcccgt
    16501 ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata gcagcaaccg
    16561 tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa aatgtctgtt
    16621 ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag ggatttacaa
    16681 ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc acatatttag
    16741 aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac atagccgata
    16801 cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt atggaaataa
    16861 aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc aatttgccca
    16921 tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga agaatcttga
    16981 tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat acggaaagaa
    17041 agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca ccttgcccag
    17101 caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct gtaccaaaag
    17161 ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca attagtggtt
    17221 tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat gtgacaaata
    17281 aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt ccgttttatt
    17341 ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact tttttagagt
    17401 gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt taaaaagtgt
    17461 aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata gtttcgtttt
    17521 caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg gtagacaggt
    17581 gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt attgaccatc
    17641 acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc aacttgagcg
    17701 attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat gcttagtgca
    17761 tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag aactaatggt
    17821 gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt taagataact
    17881 gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc atctggtaat
    17941 tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat cttatctgca
    18001 tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac ttcttccata
    18061 atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa gtcgcattct
    18121 gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac ggtatcatcc
    18181 acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata aacagattct
    18241 ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac atcccgatta
    18301 tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat attgacatct
    18361 tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa cggaacattc
    18421 catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg gctttcatga
    18481 tacagttggt tcaaggaagt agcgagcttt ccacttcctg ggccaggagc tgtcactaca
    18541 acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc tgagacgatt
    18601 ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt gatgtttcgt
    18661 ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg agtgataacg
    18721 acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa gatatccata
    18781 tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc agcatagacg
    18841 caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt cgcatcttct
    18901 tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctagcttccc accaaattct
    18961 aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg ctcttcaata
    19021 tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt actaaagcat
    19081 tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa aaaacaaaga
    19141 aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt tttgataagg
    19201 aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact atatccgttc
    19261 gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat tgacgtttac
    19321 ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc atttcacaac
    19381 aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga ttctgtccta
    19441 tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt gcagcctgtt
    19501 ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg tactattgaa
    19561 gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa gagccagata
    19621 caaatgcaaa ttatgagcca gacgaactag aggagacgaa tgcggatcag gatacaggac
    19681 aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt tcccaattat
    19741 tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct gagatctacc
    19801 gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa aatcacgaga
    19861 tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt ccttttatgg
    19921 aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact tattaccatg
    19981 atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg cctaagatcg
    20041 atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa aaaatcaatg
    20101 agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat gaacaaataa
    20161 aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg aatcgtaaaa
    20221 aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct aatgaaaaat
    20281 taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa gaagcattga
    20341 agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa caaaggcaaa
    20401 tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa ttcttgatga
    20461 attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc gagtatgaat
    20521 gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca tgaagaaacg
    20581 aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga aatccctatg
    20641 ctagaggaaa caaaaataca cgaaaaagaa aaagtaactc cgacgaatac gaatactgca
    20701 gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg aaaagagcaa
    20761 gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc ggatttacgt
    20821 atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata tgatcgtttt
    20881 atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga atacttcacg
    20941 tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc agcttatgac
    21001 acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa cgaattagtc
    21061 ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat gagtctccta
    21121 aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct ttctttgatc
    21181 cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt caatcacaaa
    21241 ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca gacattacta
    21301 gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac gaatgaagta
    21361 ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga aaagatcaac
    21421 gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac aacaaacgaa
    21481 aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt cattgatttc
    21541 tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt ggataagttg
    21601 gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt tatccagaat
    21661 aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc tgatgaagga
    21721 gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt gtctgctgct
    21781 tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat gactgctatt
    21841 tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat gagtctttct
    21901 attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca atggatcagc
    21961 tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt gcaaaggaat
    22021 ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat aggtatgacc
    22081 gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct tcaaggggtg
    22141 tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt tctgccagtc
    22201 gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt atagtttgct
    22261 tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata cacaatcggc
    22321 ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt tacaagaaaa
    22381 tggtcaaaac ttttcggaaa aagaagtgtt cttcaaaaaa ggcgatgatt tactgagtat
    22441 tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc ttgaagggca
    22501 ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga aaagtgctac
    22561 tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca atttgtctaa
    22621 actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca gtgagttcag
    22681 ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt tttccgtaat
    22741 tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct atgcaatcag
    22801 tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca gcaatcagat
    22861 tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc atcaatttgg
    22921 tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt tcttcggcgg
    22981 tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc atgaagtggg
    23041 taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag gaagttcgtt
    23101 acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca cgatgcgctt
    23161 tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact tgtgtgctgt
    23221 aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg ggattgctgc
    23281 atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg ccattttcct
    23341 agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg atttatcaaa
    23401 aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa aaacagttca
    23461 tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta acaaagagaa
    23521 tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc ttcctgacaa
    23581 gaagatgtat atatacattg aaacagataa atatgaccca aattattctc gtaaataaaa
    23641 cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt aaataaaacg
    23701 gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg taattaaagg
    23761 agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc atgcagtgat
    23821 gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt tgcaaaaagt
    23881 tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg gtggcgcgca
    23941 gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat tattgatggt
    24001 tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt ttatgaaaaa
    24061 agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa actatgcggt
    24121 gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt tatatgtcaa
    24181 ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt tcggctcggt
    24241 gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca ttttcatgtt
    24301 tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag gcgtattagg
    24361 ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa cagtcattgc
    24421 ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta agaaactaag
    24481 agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg aggccaatca
    24541 ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg gcatatatcc
    24601 cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg ttcagtgact
    24661 ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat gttttcatta
    24721 ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt ctagtagcag
    24781 gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt gcgtctgtct
    24841 ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg cgacataagt
    24901 tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg caagtttatt
    24961 cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta ttttaggaat
    25021 tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt ttattttatg
    25081 aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca ttttggctaa
    25141 cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc tggctaagta
    25201 tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt ggctatcttc
    25261 cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt gcaaaaaaag
    25321 tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt agaagtacga
    25381 tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta tttgctcctt
    25441 tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct atcagaagtt
    25501 tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat ttccaaggaa
    25561 aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg cgagtgatcg
    25621 ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata ttagaagctg
    25681 tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc catatgtata
    25741 tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc agccgttatt
    25801 tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta ccgtttattt
    25861 ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg aaaccactgc
    25921 ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc agccgtgctt
    25981 ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca gtagctataa
    26041 gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc gaaaaaacag
    26101 taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact ggaatgtctt
    26161 tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt gtcggatatt
    26221 tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta tctacgatgc
    26281 ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca attatcctga
    26341 aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg agcttatcaa
    26401 atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc aaagaatacc
    26461 gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc tccaccctga
    26521 ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg tcaatggaat
    26581 caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc ttagtatttt
    26641 tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa catcatagca
    26701 gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg gcaactcgta
    26761 tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat cttacttatg
    26821 atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac atgaaacttg
    26881 cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat cccgacttat
    26941 tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga atcgatatcg
    27001 aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact acttcacaac
    27061 ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg tatgacacga
    27121 ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta tttgcgatga
    27181 aagatgacat cagcggagta acggtcattc cttatgactc taagatcact agtatgccga
    27241 tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc cttgatgaaa
    27301 tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt gtagatgatt
    27361 tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt ggcagcttat
    27421 taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc gaaaaaatcc
    27481 gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga aataacgaaa
    27541 tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta gcgattgcgg
    27601 agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt catttcggac
    27661 cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca gcgcaataat
    27721 tccttttatt caaaaaacat accaaaaata
//
LOCUS       DAJSBM010000029        27649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJSBM010000029 DAJSBM010000000
VERSION     DAJSBM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.029"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(214..1158)
                     /locus_tag="ONT67_001502"
     CDS             complement(214..1158)
                     /locus_tag="ONT67_001502"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCR2355249.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1454..2398)
                     /locus_tag="ONT67_001503"
     CDS             complement(1454..2398)
                     /locus_tag="ONT67_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355250.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2619..3833
                     /locus_tag="ONT67_001504"
     CDS             2619..3833
                     /locus_tag="ONT67_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355251.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3855..5870
                     /locus_tag="ONT67_001505"
     CDS             3855..5870
                     /locus_tag="ONT67_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HCR2355252.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5953..7326)
                     /locus_tag="ONT67_001506"
     CDS             complement(5953..7326)
                     /locus_tag="ONT67_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2355253.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7352..7636)
                     /locus_tag="ONT67_001507"
     CDS             complement(7352..7636)
                     /locus_tag="ONT67_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355254.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7638..9653)
                     /locus_tag="ONT67_001508"
     CDS             complement(7638..9653)
                     /locus_tag="ONT67_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCR2355255.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9832..10137)
                     /locus_tag="ONT67_001509"
     CDS             complement(9832..10137)
                     /locus_tag="ONT67_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCR2355256.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10226..11257)
                     /locus_tag="ONT67_001510"
     CDS             complement(10226..11257)
                     /locus_tag="ONT67_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2355257.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11464..12339
                     /locus_tag="ONT67_001511"
     CDS             11464..12339
                     /locus_tag="ONT67_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2355258.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12491..13333)
                     /locus_tag="ONT67_001512"
     CDS             complement(12491..13333)
                     /locus_tag="ONT67_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2355259.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13425..15302)
                     /locus_tag="ONT67_001513"
     CDS             complement(13425..15302)
                     /locus_tag="ONT67_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2355260.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15299..16720)
                     /locus_tag="ONT67_001514"
     CDS             complement(15299..16720)
                     /locus_tag="ONT67_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2355261.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16723..20112)
                     /locus_tag="ONT67_001515"
     CDS             complement(16723..20112)
                     /locus_tag="ONT67_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR2355262.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20369..21760
                     /locus_tag="ONT67_001516"
     CDS             20369..21760
                     /locus_tag="ONT67_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355263.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21833..22480)
                     /locus_tag="ONT67_001517"
     CDS             complement(21833..22480)
                     /locus_tag="ONT67_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR2355264.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22488..23174)
                     /locus_tag="ONT67_001518"
     CDS             complement(22488..23174)
                     /locus_tag="ONT67_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355265.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23180..24253)
                     /locus_tag="ONT67_001519"
     CDS             complement(23180..24253)
                     /locus_tag="ONT67_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355266.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24475..25149
                     /locus_tag="ONT67_001520"
     CDS             24475..25149
                     /locus_tag="ONT67_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355267.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25154..26494
                     /locus_tag="ONT67_001521"
     CDS             25154..26494
                     /locus_tag="ONT67_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2355268.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26643..27629)
                     /locus_tag="ONT67_001522"
     CDS             complement(26643..27629)
                     /locus_tag="ONT67_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCR2355269.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8055 a         5238 c         5021 g         9335 t
ORIGIN      
        1 tttaattaac cacagataaa cattgataac gattcgctta ttgtaatcaa cagattaaat
       61 ttcatatgtt catatgtacg gatacatttt cggaccgatt aattttaacc attctgttta
      121 tttgcttacc tattagatat caataaaaaa ctgcttaaca gctatttcag ctgttaagca
      181 gttttaggat ttttagttca tattcattgt ttcttataat acagtgttca atacttctct
      241 taaagcactt gttgatttgc tgaacattgc tttttcttct tcgttgattg ataattcaac
      301 aacttcacga acaccatttt cgtcaacgat tgatggcaca cctgtaaaga tatctttttc
      361 gtcgtattca ccagttaaat aagcagatac aggcaataca gcttgttcgt tattcaagat
      421 agcttttacg atacgtgcag tactcatacc aataccatag taagttgctt ttttacgatc
      481 aatgatctca taagcagcat tacggacttt atcagcaatc acatctaatt catcttgggc
      541 aatacgatgg tctttttcta caatttcaaa cactggttta ccaccgacag ttgtatgtga
      601 ccatgcagca acttctgaat caccatgttc acctaagata tagccgtgga cgctgcgtgg
      661 gtcaactttt aatttcaatg cgatttcttt acggaaacga gttgtatcca atgttgttcc
      721 agtaccgata acacgtgatg ttggcagacc agattcgttc caagcgatat atgtcaaaat
      781 atctactggg tttgaagcaa caacgataat accgtcgaat cctgatccca tgatttcttt
      841 tacgatttgg cgcatgattg acgcatttgt tttaactaaa tctaaacgag attgaccagg
      901 tttttggttg ataccagctg tgataacaac gatatcagca tctttacatt cttcatagcc
      961 acctgaccat acagccacat tttcatcgcc ccaagccata ccatctaaaa ggtccaatgc
     1021 ttccccttct gctttttctt gattcacatc gatcaatact aattcattag agattccttg
     1081 gttgatcatt gcataagcga tacttgttcc aacaaaacct gtaccgacga ttactacttt
     1141 acgacttgtt tttttcatga ataaacagct ccttttcata actatttgtt actttcatga
     1201 aaagtatagt cttgtttatt cattttatca acatgttaat tataatagtt gttattataa
     1261 tttttgatgg aaacgaattc ctgaatacgc ataacagata aatgacaaat gtttacacct
     1321 atatcccctg ataatacaac caaaagttta gaactgaaaa taaatccaaa aaaattcgct
     1381 gcaacgatat aataagacag caatcgcacc taaaacaatt ttccttcata tgctttagtg
     1441 aaagattttc ttcttaatgt ttatttctcc attcattggg agtacatgtt gaatacttct
     1501 tgaataattt gaataaagaa gtcgtttgtt tatatccgat acgattagcg atttcttcca
     1561 acgaaaaact cgtttcttga atcagttcca acgcaaagtc gaattttttc tttagaacca
     1621 gttcttgaaa ggaagcatca aaatacattt tcaatttatt actaagatag accggattgt
     1681 agccaaaatg agctgctgtt tcttttaacg tacaagaagt aaaatgctta tcgatataat
     1741 ctaatactct aagcatctca ttttctgcgc atccatctgc tgatgaaaag ctttgttgtt
     1801 ctaataaatg agtcaattcg atcagtaata gtgatgtata gagatttaac gaacggatct
     1861 tatgactgta tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc gtcaaacgac
     1921 ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct tttgatgcat
     1981 ttatcaaaaa agaagaaagt agattatttg tgcttgccaa atgattcaat atctctgtag
     2041 ataaggtttc ttcttttaat aagatgttga ttaaaatatc ctcttgccct gtgtattcga
     2101 ttctttgagt agcttcttta tcaatgatca ccacgtcact ttcttccatc tctatccttt
     2161 ctccattaat aatttgtaaa catgtaccgc tatacatata attgatttca acaaaatcat
     2221 gtgtatgctc gggaacttcc gcatatcgac ggtgtttact gatatgaata tttccctgtt
     2281 gaaatagatc ccctcggtgg atttttggcg gtgtctgaag atggtaagct aagtttaaat
     2341 cattgatatt cattccttgt tcacgctgca gagtttcgat tgattcttcg ttacgcaaaa
     2401 aaaatgtcta attcagctag ttccaattga ctccctcctt tttgtaagcg gtttctttcg
     2461 cttattagtt cacgctgtac tgctattata tgcaaagcta tttttggttt cataaagact
     2521 ttctatggtt gggacatgaa cttcctgtga ttccgctatt gagagaataa ccacttcata
     2581 ctatattttt gataagcgct tacaaaaatg aaaggaacat gcttatgaat aaaaagatga
     2641 tgaaattttc tctgctgtca gtctctctta ttttgacttc tgctggtgca gtcacgtctg
     2701 caattcccag tatgtcagct acttatgcca cgcgttcttt atctgctgtt gaattattaa
     2761 cgactgctcc ctcttttatg gtcactattt ttgttttgat tagtagtttt attgctagga
     2821 aaatcggaca aaaacaaact gtgatcatcg gattagtgat tgcatcgatt tgtggtatcc
     2881 tccccctctt ctcacaaagt tacagtattg tcttagcttc aagagctgga ctaggtattg
     2941 gctttggatt gatcaattct ttggcagtca gcatgattgc tgccttttat cacggagatg
     3001 aacgagcctc tatgattggt tttcaaagtg catttcaagg atttggtgca gctttaatga
     3061 catttgttgc aggacagcta gtacagttcg gttggcaata taccttttta gtctacgcca
     3121 tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa acaactgaac
     3181 ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa aacgaatcct aaaatgatta
     3241 gttatggtat ttttctcttg ctggtcatcg tggtatataa tgctgtatcg attaaattag
     3301 ccacagtcct gacgacttat tctctaggaa acgaaactaa tgcagcgaat atcttaacaa
     3361 tcatgcaatt ggcaagtatg gctgccggag tttgttttgg ctggcttcag acaaaagcac
     3421 atcgctatat gcttacactt tctttactac tgatgggaat cggctttgta ttgattgcca
     3481 ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct aacaggaatt tctttttcgt
     3541 tattcattcc ttacttattc aaccaagtaa gtatccaagc gcccaagtac gcggagagtt
     3601 tcaatacttc aatcttattg gttggcgcta atttaggtac tttcattgca ccatatggtt
     3661 tgctctttct ctcttttgct tctcttggga gtaaaacact tgctgttttc gtcaatggca
     3721 gtatcttgct gttgattagc gcgcttatta gccttctagt agtacacagg acgaatagaa
     3781 agacagtgat aagagtagat gaagaaataa aagaacaatt gacaacaaaa taaatttgat
     3841 ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag atggctttgg tatcctggag
     3901 actttgagat ccgccatgga ctgctacaaa atttccaacg ggaagaacga ggttttgact
     3961 ggccagcgta ctggtacatg gatgattgcc atcggaacgt caagtttaag agatattact
     4021 ttcttgatca accttccatg tttaaagtga cgatccaagg agttgggtat gtagaaatta
     4081 atggacaaaa acatccatgt ggaaaatggc tgacttgtcc agctggtaaa gcaaaaattc
     4141 gtatctttgt aggacataca tcagggctac cagcgatgtt tatcgaggga gacgaagtaa
     4201 aatcagatat tggctggaca gcaagtaatt ttattgaaga atatccagct ggctggagtc
     4261 cgctttatgt cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa gaatgcgtcc
     4321 atccagtcac tgaaacggag attggcggag gaatcttatt cgattttggg cgagcagtga
     4381 atggctgtgt agaactaata tctctggatg accatatcga atctatcact ctatgttatg
     4441 gagagtccga tgtagaagct atggatatcg attattgcta ctataagcaa ataggaatga
     4501 caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt gtttatacca gaggtttcta
     4561 ctggaatggt tcaacttcaa gtttttcatg aatttttatc tctttcgaaa caagctgctt
     4621 tttcaagcga ttcttcttta ctgaatcaga tctggtcagt atcagaagaa gccttcaagt
     4681 tatgtagtga tttgtttttt atcgacggta tcaaacgaga tcgctggatt tggtctggag
     4741 atgcgtatca atcattcatg atcaatcaat atcttttttt tgacgaggaa atcaataaac
     4801 gaacgatatt agcattaaga ggacaaaatg aaatcaagca gcatatgaat actatcgttg
     4861 actattcgat cttgtggttg attggtatcg aaaaccacta tatgatgacg aaagactatg
     4921 aatttttacg catcgtctac cctaagatgc aatcattaat gagatacttg atggagcaaa
     4981 cgaatgaatt agggtttatt tatgggagag caaaagattg gatatttatt gattggtctg
     5041 aaatggataa agaaggaacg cttgctgcag aacaagtctt attactgaaa gcctatcatt
     5101 ccatgatcac ttgcggagat atcttagctg aaacatatgg agagaacgaa aattatcata
     5161 ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac tacttttggg
     5221 atgaagaact tggcgctttt atcgattgct atgaatctgg gagaagaaat gtgacgcgtc
     5281 atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa agaccaacag caatcgatct
     5341 taaagaatgt cttattgaat gacactatca cacagattac tactccatat ttcaaattct
     5401 atgaacaaga tgctttatgc aaactcggac agacgaatat cgtctaccaa acgattcttg
     5461 attattgggg agggatgtta tctcatgatg cggtaacttt ctgggaagaa tacgacccta
     5521 ctcaaacagg aaatgaaaag tacagtatgt atggagatcc ttatggaaaa agcttatgcc
     5581 atgcatgggg agcaagccca atctatcttt taggacgcta ttttatcggt ttgtatccaa
     5641 caaagccagg ttatgaagaa tttatgatac aacctaatct agattcattt gaaacactga
     5701 attgtacgct acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa gataagatcg
     5761 tgattacttc tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt tatcctcttc
     5821 tagcaaaaca gccacttgtc ttaacaagct attcaaaaat aaatgcataa aacaaaaaac
     5881 tgcttgcgac atatcagaag actttaaatc ttcttctgat attgtccaag caatttttat
     5941 tttgaagttt gcttaattct gttctttaga aacaatgttt gttctttttc cggcaaaaag
     6001 aaagctagat aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca aggctatttg
     6061 accgtattct cctagtttcc caagtacaag tcctggtact agataatcag tatcggcaaa
     6121 tgtagaagaa gcgattttaa gatctcctaa tactggaagg ataaataatg gcagccaact
     6181 aatcaataag ctattgaaag cagaacctaa taccgcccct cttctaccgc cagtcgagtt
     6241 gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct actaccccag gaatgataac
     6301 tgtagtttgc aagcctatca tagcaaacat ggatagtgtt cctacaaaga agctaacaaa
     6361 aaagccaatt aatacagcat taggtgcata tggaaaaacg ataggaacat ctaatgctgg
     6421 tttagaatcg ggtacgagtt ttttagcaat cccttggaat gctggaacga tttctgccaa
     6481 caccattcga accccttgaa ggacgataac aaacccagct gtgaatgttc cagcttggat
     6541 cagtgcaaag attacagcgt tgtctccatt gcttagttct ctttcaacaa atccaggacc
     6601 agctaacaaa gcaagtgcga catatacgat cgacatcaag agcatgatcg atacagtaga
     6661 atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc tctgtagaac ggtctttgtt
     6721 tccaaaaaac tttccgatcc atccactaga agcataaccg atatttccgg tatgccctaa
     6781 cgcaacttga ttgtttccag tgatgttctt cataaacggt tgacaaagag caggcgtaat
     6841 agttaatagt gttccttcga agaccccgcc taataagatc atccacaaat ttgcagtaaa
     6901 ccccgcgctt actaaaatga ctgctgttag acaagaaaca taaagcattg cttgacctgt
     6961 aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa atattcacga tcatccctac
     7021 tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca ctactgcttc
     7081 attgctagga actactcctt gagtatgcag agctgtttgg aacatcgaag caaaaggggt
     7141 tagtgaatta ctgattaaac cagcaccgcc gatcagtact aggaatccga caaatgtttt
     7201 gatagttcct tggatcactt ctgaaaattt cttcttctga agaacgagac caagcagtgc
     7261 aatcaaagaa acgaggatgg agggctgact gacgattgac acaaacaaat tcaacaattt
     7321 atccattgat agttcctccc ttgaatcatt tttattcttt tgacaagatc ttcgttactt
     7381 tttcttttac ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta
     7441 tagaagttag cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag
     7501 atacacttcc aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg
     7561 caagtgcttg tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata
     7621 ctgcaaaaat tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt
     7681 gttgatgcgt gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt
     7741 gccaaagccc gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg
     7801 ggatgggctg gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct
     7861 acttcgttta cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg
     7921 ataaacgcac catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt
     7981 ttattttctg ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt
     8041 tgatccgata attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc
     8101 tccttctttt ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg
     8161 tctacgcctt cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa
     8221 ggcattgaaa atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt
     8281 tccaagtcag tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg
     8341 agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca
     8401 gctgaattag tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag
     8461 atcaatgaag agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt
     8521 tcttctgcgt ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct
     8581 tcttcaggga tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt
     8641 tcgtagatgg aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa
     8701 gaataggaaa tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca
     8761 cgaaacgaag caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa
     8821 cattccagca aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag
     8881 caaagagaga aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat
     8941 acttgtcgac taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt
     9001 tccatggatg tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc
     9061 attcgatttg gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta
     9121 actttggcgt aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca
     9181 gcaagtgact gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct
     9241 gaccctagca gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct
     9301 aattcatttc ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa
     9361 tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga
     9421 tctcttcgct cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct
     9481 gcccagtcga ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca
     9541 aaaggagtta attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt
     9601 tctagactga tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg
     9661 ttcacctcct acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca
     9721 acttacagtt ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa
     9781 aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg
     9841 atcatgttta ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt
     9901 ttcgatatct ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt
     9961 tgaaccagat aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga
    10021 ttcttctttg tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt
    10081 tttaggggcc attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt
    10141 ggttcctcct agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa
    10201 aacaaacccg attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga
    10261 ttccttcttc caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg
    10321 cgccattaac taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg
    10381 aaattacttt ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat
    10441 gttcgggcaa atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt
    10501 cttcttgtcg atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac
    10561 ttggacgttc tttgatgcgg aaataactgg attttatcga aattttcata tttccataga
    10621 aaaaatccag atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat
    10681 agctgatatc atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat
    10741 gtccatataa atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc
    10801 tgaaataatc ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta
    10861 ctttttggac tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata
    10921 gacctttttc ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag
    10981 gtttctctac tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa
    11041 attgactagg tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca
    11101 gctgatcagt atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa
    11161 tttttttgat attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg
    11221 tactctttcc attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat
    11281 gttttcagta tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa
    11341 gttcagaaaa acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa
    11401 aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag
    11461 atcatgctta ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg
    11521 attgtcgatt acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta
    11581 gcaaaagcta cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc
    11641 agcggattta aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag
    11701 ttcaatcaaa cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt
    11761 gcaaagaaaa tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa
    11821 catgaagatt tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt
    11881 gcaaaaggtg attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat
    11941 aagtttttga tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact
    12001 agcgaggatt gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata
    12061 atgacctatt tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca
    12121 gaaatggcca aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct
    12181 aaagtagcga ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca
    12241 gtgatatatt ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg
    12301 atgcaaaaag gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa
    12361 taagaaagat agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt
    12421 tctttttcaa acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa
    12481 tgaggaatag ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa
    12541 ggaggaaaat cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg
    12601 atactttctg tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga
    12661 caagtaagcg atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct
    12721 gccctttttc gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga
    12781 cttgataagc gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt
    12841 tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat
    12901 gtgtattttc tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta
    12961 gcagcacagt attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca
    13021 aaataccgat cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct
    13081 tagtaaaggg atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg
    13141 tcatgttttc ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat
    13201 aatgagagat gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag
    13261 ctagtatccc tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt
    13321 tctttggttt catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc
    13381 atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc
    13441 ttgcgtctag caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac
    13501 acatagattc ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt
    13561 tcaggagaaa tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca
    13621 cggtttgtta atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca
    13681 tattttagac ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat
    13741 tctttcgccg atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct
    13801 gtgtcactat cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta
    13861 acgtcaccat ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt
    13921 gtttgatcat ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga
    13981 tctgcttttt cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat
    14041 gaaggaatat aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa
    14101 taatttactg ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa
    14161 gaatcattat caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac
    14221 ttgttttgcg ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat
    14281 tgaatttttt caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact
    14341 ttagacgtat catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca
    14401 aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct
    14461 aaattatatt ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc
    14521 tcggcaaagt cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg
    14581 gtttgatcag ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg
    14641 cttggtgttc cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg
    14701 gcagtaccga tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag
    14761 agatggatag tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc
    14821 atctcatata cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt
    14881 tcttctttga tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg
    14941 acatttccat cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg
    15001 actgcttgtt ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga
    15061 gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc
    15121 atttctttcc cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga
    15181 aggatgactt gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg
    15241 agtaatggta aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc
    15301 attttttcac tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca
    15361 taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc
    15421 tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa
    15481 aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga
    15541 tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat
    15601 cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt
    15661 gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat
    15721 ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct
    15781 ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct
    15841 gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt
    15901 ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc
    15961 cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg
    16021 cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg
    16081 tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta
    16141 ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga
    16201 tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg
    16261 ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt
    16321 tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg
    16381 tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt
    16441 gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat
    16501 aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc
    16561 cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa
    16621 tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta
    16681 acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc
    16741 ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat
    16801 tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc
    16861 taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc
    16921 cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta
    16981 tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag
    17041 aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag
    17101 tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc
    17161 aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt
    17221 ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga
    17281 agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga
    17341 atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc
    17401 atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt
    17461 cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt
    17521 caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc
    17581 agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt
    17641 tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata
    17701 ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac
    17761 agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg
    17821 tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg
    17881 tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg
    17941 tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc
    18001 acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt
    18061 ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg
    18121 atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg
    18181 agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa
    18241 agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg
    18301 gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct
    18361 cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac
    18421 actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat
    18481 ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat
    18541 atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat
    18601 ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag
    18661 ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat
    18721 ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata
    18781 gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc
    18841 gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac
    18901 accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc
    18961 tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga
    19021 ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct
    19081 atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc
    19141 tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc
    19201 gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg
    19261 tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc
    19321 atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg
    19381 ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt
    19441 tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg
    19501 ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact
    19561 cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt
    19621 tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt
    19681 ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct
    19741 gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga
    19801 gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct
    19861 ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact
    19921 ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac
    19981 ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa
    20041 tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc
    20101 tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa
    20161 ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg
    20221 aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga
    20281 ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat
    20341 caggttgttg gaaggaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc
    20401 tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt
    20461 tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc
    20521 acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat
    20581 ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg
    20641 taaaacgatt gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa
    20701 gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg
    20761 ttttcagtta actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt
    20821 tttaagttac tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga
    20881 aattatggtc aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca
    20941 atctttcttt attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt
    21001 tgaatggaaa gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt
    21061 aatcttgaaa aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta
    21121 ttttgtcaat acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca
    21181 gatacgaaaa aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga
    21241 agaatatgtt gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga
    21301 tacgactcat ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat
    21361 acgtctatat gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa
    21421 tctgatttat tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca
    21481 ccattatact tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca
    21541 atatattgaa gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac
    21601 tgctattact gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac
    21661 gaatcaaccc attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt
    21721 cgcaaatgaa gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg
    21781 tataaaccac cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta
    21841 ttccttcagc aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc
    21901 aattatttgc aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga
    21961 aactaactgc ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc
    22021 atttttcgtt cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat
    22081 ttcgctgggt taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc
    22141 accactctaa atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt
    22201 ttaagatatc ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt
    22261 ccaaatcttg aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt
    22321 ctgagacttt gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga
    22381 ttctctgttt ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc
    22441 ctttatctct gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt
    22501 ttctcttctt ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc
    22561 atacggacat catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact
    22621 agtaatttga cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct
    22681 aagatcaagc ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca
    22741 ccagaaaggt ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag
    22801 gaatcgattt ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt
    22861 tcctttaccg ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta
    22921 aagcgtaaat ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact
    22981 atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct
    23041 gaaccagaag gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct
    23101 ttcaaagctg agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt
    23161 aatatttttt tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg
    23221 tacgtatcga aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca
    23281 accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat
    23341 aagcaaacaa aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta
    23401 aagatttagc gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag
    23461 tcttctgaag tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca
    23521 aaaagtatat catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa
    23581 tgaatgtccc aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag
    23641 ctgaatcctt tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt
    23701 gtccatactt caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt
    23761 aataagtttc aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga
    23821 tcgtatctcc aattttatag cctgaatcag ccagattttg agagatgatg atttcattct
    23881 ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc
    23941 cgaaaactgt aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg
    24001 gtgctttctc tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa
    24061 atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt
    24121 ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa
    24181 tcaagaacat aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga
    24241 tagctagaaa catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg
    24301 ttgaatagtg taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata
    24361 aattgtctaa gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc
    24421 cttatttatg ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt
    24481 aaactattag tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct
    24541 gcaatcggcg agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt
    24601 ggagtttatg atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg
    24661 ttagcaaaaa tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat
    24721 agtttggaag ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct
    24781 ttctatcgag aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc
    24841 tttgatgaaa atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc
    24901 ttgtataaag aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt
    24961 ttacaaaata agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt
    25021 gattctgata cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg
    25081 cgaagtactg gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa
    25141 agggtgtgat gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac
    25201 aaatgtagca gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt
    25261 aaaccaatct gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg
    25321 aacagtacaa ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca
    25381 aacggaacaa aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt
    25441 taacactcaa gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg
    25501 tgggcgtttc aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca
    25561 aaatattgaa ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa
    25621 aaaagcggag acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca
    25681 aatcaagaat tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg
    25741 gattctctct attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct
    25801 ttcttggaga agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt
    25861 aacgattatt caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga
    25921 agaatccgaa agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc
    25981 ggatttgctg acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat
    26041 caatacacaa gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga
    26101 tggaaaagag tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa
    26161 aatccatcaa gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga
    26221 taagatcatt ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac
    26281 tggcccttct atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga
    26341 tcaatctcgg gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt
    26401 aatcgaacag catcatggac agatttctgt atccaattat cttccacaag gagtcgattt
    26461 taaagtccaa ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga
    26521 caaaaatctt gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct
    26581 tcggaaataa gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac
    26641 gcttacgatt ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat
    26701 tcttctcggc taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc
    26761 ccaacttctt cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg
    26821 atagtaatcg tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact
    26881 ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg
    26941 ttgaccacat gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct
    27001 aatttttctt cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct
    27061 tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct
    27121 ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt
    27181 ggatctgttc cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta
    27241 tcttgtgtat ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg
    27301 acaaattttc catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta
    27361 gccgtctcag aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa
    27421 cggtcttctt caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg
    27481 tcaaatccta gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca
    27541 tacccttcgt tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt
    27601 ccgatgaccg ttaaattttt ctcttccatg taaattcctc ctagcaaat
//
LOCUS       DAJSBM010000030        25650 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJSBM010000030 DAJSBM010000000
VERSION     DAJSBM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.030"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            133..3660
                     /locus_tag="ONT67_001523"
     CDS             133..3660
                     /locus_tag="ONT67_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2355270.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ONT67_001524"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ONT67_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCR2355271.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ONT67_001525"
     CDS             7448..7789
                     /locus_tag="ONT67_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2355272.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ONT67_001526"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ONT67_001526"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR2355273.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="ONT67_001527"
     CDS             9146..11566
                     /locus_tag="ONT67_001527"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR2355274.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT67_001528"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ONT67_001528"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR2355275.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ONT67_001529"
     CDS             13000..13737
                     /locus_tag="ONT67_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2355276.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ONT67_001530"
     CDS             13751..14566
                     /locus_tag="ONT67_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCR2355277.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ONT67_001531"
     CDS             14579..15226
                     /locus_tag="ONT67_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2355278.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ONT67_001532"
     CDS             15242..15898
                     /locus_tag="ONT67_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2355279.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ONT67_001533"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ONT67_001533"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR2355280.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ONT67_001534"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ONT67_001534"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR2355281.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ONT67_001535"
     CDS             18240..18758
                     /locus_tag="ONT67_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2355282.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="ONT67_001536"
     CDS             18865..19365
                     /locus_tag="ONT67_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2355283.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ONT67_001537"
     CDS             complement(19645..20565)
                     /locus_tag="ONT67_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCR2355284.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="ONT67_001538"
     CDS             20730..21743
                     /locus_tag="ONT67_001538"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCR2355285.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT67_001539"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ONT67_001539"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR2355286.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT67_001540"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ONT67_001540"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2355287.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ONT67_001541"
     CDS             complement(23383..24144)
                     /locus_tag="ONT67_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2355288.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ONT67_001542"
     CDS             24333..25427
                     /locus_tag="ONT67_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2355289.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25650)
                     /locus_tag="ONT67_001543"
     CDS             complement(25513..>25650)
                     /locus_tag="ONT67_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2355290.1"
                     /translation="RKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSL
                     I"
BASE COUNT         8493 a         4443 c         5408 g         7306 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct
//
LOCUS       DAJSBM010000031        25166 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJSBM010000031 DAJSBM010000000
VERSION     DAJSBM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.031"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            510..1154
                     /locus_tag="ONT67_001544"
                     /pseudo
     CDS             510..1154
                     /locus_tag="ONT67_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            1382..2671
                     /locus_tag="ONT67_001545"
     CDS             1382..2671
                     /locus_tag="ONT67_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2355291.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2756..3628
                     /locus_tag="ONT67_001546"
     CDS             2756..3628
                     /locus_tag="ONT67_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355292.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            3618..4433
                     /locus_tag="ONT67_001547"
     CDS             3618..4433
                     /locus_tag="ONT67_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355293.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4449..5573
                     /locus_tag="ONT67_001548"
     CDS             4449..5573
                     /locus_tag="ONT67_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355294.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5551..6354
                     /locus_tag="ONT67_001549"
     CDS             5551..6354
                     /locus_tag="ONT67_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2355295.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6805..7077
                     /locus_tag="ONT67_001550"
     CDS             6805..7077
                     /locus_tag="ONT67_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2355296.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7067..7396
                     /locus_tag="ONT67_001551"
     CDS             7067..7396
                     /locus_tag="ONT67_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2355297.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(7575..8789)
                     /locus_tag="ONT67_001552"
                     /pseudo
     CDS             complement(7575..8789)
                     /locus_tag="ONT67_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8990..10264
                     /locus_tag="ONT67_001553"
     CDS             8990..10264
                     /locus_tag="ONT67_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2355298.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10647..11375)
                     /locus_tag="ONT67_001554"
     CDS             complement(10647..11375)
                     /locus_tag="ONT67_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR2355299.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11353..12927)
                     /locus_tag="ONT67_001555"
     CDS             complement(11353..12927)
                     /locus_tag="ONT67_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2355300.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13156..14094
                     /locus_tag="ONT67_001556"
     CDS             13156..14094
                     /locus_tag="ONT67_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355301.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14106..15017
                     /locus_tag="ONT67_001557"
     CDS             14106..15017
                     /locus_tag="ONT67_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355302.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15041..16513
                     /locus_tag="ONT67_001558"
     CDS             15041..16513
                     /locus_tag="ONT67_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2355303.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            16564..17175
                     /locus_tag="ONT67_001559"
     CDS             16564..17175
                     /locus_tag="ONT67_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2355304.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            17205..19118
                     /locus_tag="ONT67_001560"
     CDS             17205..19118
                     /locus_tag="ONT67_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HCR2355305.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            19246..22227
                     /locus_tag="ONT67_001561"
     CDS             19246..22227
                     /locus_tag="ONT67_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HCR2355306.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            22224..23885
                     /locus_tag="ONT67_001562"
     CDS             22224..23885
                     /locus_tag="ONT67_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HCR2355307.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            23925..24857
                     /locus_tag="ONT67_001563"
     CDS             23925..24857
                     /locus_tag="ONT67_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCR2355308.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         8107 a         4053 c         5142 g         7864 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaaatc tggccacaaa ttgttcttag tggctatgta caattatctt
      841 gggctgcgat ttggggaaag catatatttt ataatattga aaatcagttc gaagggaaaa
      901 aacttgggga tattaccgaa gcagatttga tcgatacctt acagaaatta gcctatcaaa
      961 gcgttcaaat cgtcaatatt catgaaggat ggttaactaa gttggcccgt gaagtggggc
     1021 agcagctagg attgacattt tctgtatgga cagtcaatga actagaaacg attgagtacc
     1081 ttgcgcgctg tggtgtttat aatataacct cacgttgtgc atgggcctat ttatggaaga
     1141 aaggagcaca gaataaacta ttttataatc aaaacagagg agttaagaga tttggagatg
     1201 ggatacatga ccatatgggg gctttattct tagcttgatg gatatgtcca gtgaccctgt
     1261 accccgaaat gcacaaattt cgaattattt acagaaaatt aactagaaat ttacattggt
     1321 agttcataat atttcttgat tagaaaaatg gccataaaga aattaggagg aagttaatgt
     1381 tatgttaaag aaaaatcttg ttataggggt tgtgtctgtt ttaagtgtca tctctttggc
     1441 agcttgtggt agtgggaata ccaagacaac aaaaaaatcg gagggtcgtg taacgataga
     1501 gtattggcat gttaatgcgg ataccctagg tggaaaaaca gtgaatgaat tagtagataa
     1561 atacaatcaa tctcaagaca aagtgaaggt tgtagcaaaa tataatcctg atatgtataa
     1621 aggcttgatg caaaatttac aagcttctgt tgcctccaaa gaaataccgg acgtagtaca
     1681 agtgggatgg gcttttaaag attattttgc agaaaatttc aaatatacta atcctcaatc
     1741 tttagtagaa gaagtggatc cggataatgc tgattatttt aaagatcatt ttttagacaa
     1801 tatcatggca ctgtctgaaa aagatggtaa acaagtgggt attccttact ccattagtaa
     1861 tccggtttta tatttaaatc aagatatatt aaaagcagcg ggcttggatg aaaatggtcc
     1921 aaaaacttgg gaagaagttc aaactttttc taagataata aaagaaaaaa ctggaaagta
     1981 tggcgtgtat attcaagaac ctgctgataa ttgggcacaa caagcaattt tagaaagtaa
     2041 tggggcaagt gtaataaaag atggcaaagc tacctttgct tctaaagatg gtatcgctgc
     2101 ttatgaatta tatcaaagga tggtaaaaga ccagacggca ttacacacta cgtgggaaca
     2161 aggtgtgcaa agctttattg atggtaacgt cgcgatggtc tataccacta ttgctcaaag
     2221 ggacaacatt caaaaaaata gtacttttac tgctaaagca gttgagtcac caagttggga
     2281 agggaaaaaa gttaaactac ctgctggtgg cgcaatgtta gctgttacat cacaaacgga
     2341 taagaaaaag gaagcaactt gggatttctt aaaatatctt tatagtgttg aatctttagc
     2401 taaatggaca gaaggtactg gttatttacc accacgtaaa gatgttgcag acgcccaaaa
     2461 tggattaaaa gctttcttag ctgaaaatca gatgatgata ccagcaattg atcagatgga
     2521 taagatggtc agttggagtt catttcctgg agatgctgga ttagaagctg aacaaaaatt
     2581 gttggatatg cgggataaaa ttttatcagg taattccgtc aatgaaacga tgaaagctac
     2641 tcaaaaggaa attaatgagt tactcaaata agcaaagcaa gcagacgaca gcgcctgtaa
     2701 gaaaggaaaa acaaggtcgt ctgcttttga cataaggatg aggaggctgt cggaaatgaa
     2761 gcgctatgta ggttatttgt atctggcgcc agcaattatt ttgttgggcg tgtttgtttt
     2821 ttatccattg atttatacga tctatttgag tttttttgac tggaatatgg tggcccccac
     2881 taaagaattt gttggctttg ccaactatgt aaaggtgctg actgatccag tctttcataa
     2941 agtgttgaag aatactttgt cctataccgt tctatttgtc ttgctgagct gtgtgttccc
     3001 atatattaca tcctttttat taggaatagt tatagaaaaa gggcagaatt tgtataaacc
     3061 attatttttt attccagctg tgatttcttt ggtagtggcg gggatggttt ttacatggat
     3121 attaaatccg gtatcgggac caattgctgt cttgttggga aattttggtt taagactgcc
     3181 tatgtggatg aatttacaag gttgggtaat tgctgtgatt gcaggtatta ctgcttggaa
     3241 agtttttggt tataatttca ttgtcatttt tgcagcaatt gtgggaattg atcaagagat
     3301 gatcgaagca gctaagttgg atcgtgtacc catctggcgc atttttttta caattgtgtt
     3361 gcctatgagt agttcgacag gactatattt attggtgatg accattgttc aaggtctgca
     3421 gtatgtgttt actccgatta agatcatcac tcgtggtgga ccgaataatg gtagtgcaaa
     3481 cgtgatctat tatgcttatc aaaaggcttt tgaaatgtat aaagtcggag aatcttcagc
     3541 aatttcagtt ttaactttat taatatttgc tgttttctta ggggtaatat ttaaatttgt
     3601 ggaaaagggg gtttactatg aaaattaaac gctggttgcc acatctctta tttatgttca
     3661 tcataatcat tattatctat ccattattgt ttattatggc caattctttt aagccattga
     3721 aagatgccta tcagacgatt actagtttga taccacagcc gtttaccttg gaaaattttt
     3781 gctatttgtt cgaaaatctt ccaattatca aaattacgag aaatactttt tttattgcta
     3841 caattataac ggttgttaag ttaggcgtag ctttttttgc ggcatatagt tttacttatt
     3901 gccgaatcaa agggaagaaa atctggtact ttatcatcat tggtacgatg ttcattcctt
     3961 ttactgttgc gatgattcct aattatttgt tgattgcaaa acttaattta attgatacta
     4021 cttggggtgt gataatacca caatttgctg atgcgatggg cattttttta ttgacgcaaa
     4081 caatgcggga aattccagtg agccttctag atgtggcggc attggataac atcaaacaaa
     4141 caaccgtcat gagtaaaatt gtttttcctc ttactagaca tgcgattact tctaccggta
     4201 tttggttttt tattacctct tggaatgagt atgtttggcc ggtattaatt ttacggacag
     4261 tagataatta tactttgccg ttggcgatgc agacctatat tagttctgaa ggtgggacaa
     4321 attttaccgt agcaatggcg attagtttaa ttacaatgtt tgtaccgtta gtcttgtatg
     4381 caatttttca aaaatatatt atcgggactt ttgtttctgc aggtattaaa taaattggag
     4441 gacaaatcat gggagaaacg cttgaattaa gaaatatcgg aaaaaaatat ggagaacata
     4501 ccattttaaa agaaattgat ttagctgtgg ccgctggaga acgaattgtg ttactgggac
     4561 catctggttc aggaaaatct actttgctga gaatgattgc tggtttggaa aaaattacga
     4621 gtggagagct ttttttaggt acacaactag caaataagct accttgtgga gaacgagaga
     4681 tcgcaatggt ttttcaaaat tatgctttat atcctcacat gactgtaaag gagaatatta
     4741 tttttgcatt aagggcgaat aaaattaaga cagctgacat tcagcaccgt ttggaagaag
     4801 ctttgaagat gttaggttta acgccatacc aagatcgact tcctaaagat ttgtcaggag
     4861 ggcaacggca gcgtgtagca ttggctcgtg cggtggttaa acggagtaat tattttttat
     4921 tagatgaacc gttatcaaat ttggatgtgc gtttacgatt ggatgcacga aaagaattgg
     4981 taaaaattca tcaacgttat aaacaaactt ttgtctacgt cactcatgat caaattgaag
     5041 cgatgacgtt agctgaccgg attgttttat tgaatgaagg tagaattcag atgattgata
     5101 cgccgaaaaa tgtttatacg aaacctaaaa atacttttac tgcaaatttt attggttcgc
     5161 caggcatgaa tattatgcca gttacttatg atcaaggaac attaaaggta gccagtcaat
     5221 ttttaacatt gaatccagct tggcaaaaac aacttcttca ggttagtggc tcattgtatt
     5281 taggcataag accagaacac gtaacctttt caaaagaaac aagcgattta cacggtcaag
     5341 tgaagtatcg tgagctatta ggtcaaagtt atgcgtacac cgttaaagtc gatggtatag
     5401 aaatgattgt ccttaatgaa aaacctactt ggcagattgg tgatgcagtc aatctccgca
     5461 tagaacctga aagaatgcat ttttttgatt atcagacgca agaaaattta ggatatcctg
     5521 attattttat tgaagaaaga gggaatagcc atgcgctttt tgcatctctc tgatatccat
     5581 tttagagaaa actatcaaac taacttgttt actgctgaac tatttccaac aattaaaaat
     5641 ccacagttgg cattggaaaa attgttacaa gaactctcgt taacagtgta cgattttatt
     5701 ctattaacag gtgatttagt acatgaaggg gggatttctg attatcaagt cttacggaaa
     5761 atgttggatc aatatttccc aaccacacct tactattttt gtcgtgggaa tcatgatcgt
     5821 cgcagtgttt tctttgaagg aatgagtgtt gaaagcaatg aaacgggaga gtatatccat
     5881 tgtacgaatc aaaatgggtt acgaattatt tccttggatt ctgctcaaga tacccagcac
     5941 gaaggaaaaa tatcgatagg acagatgatt caattacaag agtggttggg tacacctgct
     6001 gaaaagggaa cgattttatt ggttcatcat ccattgtcgt gggaagatcc atctattgta
     6061 actgaagttc cggaaggttt tgctgaattg attgccgaaa gtgatatttt aggtattttt
     6121 gtaggacata tccatcaagg tagcagtgct gaatttgctg gtaaaaaaca gtttatggct
     6181 gaatctttat catttggtgt agatgaatac ccagactatt ctctttttac caatagaaca
     6241 ggattcacta gtggcagttt aattgacgga aaaattttta cttatcagca ttatttaact
     6301 ccacatcaaa tagaaatagg gcgcatgaaa aaaccaatta ctggtaatca ttaaaaatta
     6361 ttgattaaaa ataaagactt cattcacttt ttagccgtgg tgctcaatgt ctggttccac
     6421 ttgaagaact tggaaaatcg ctatcaataa accactatcc ctccaattac aaaaagggtg
     6481 gagaaagttt tccaagaacc atatcggtct tcgtggttgt gccattcgtt ttttaccaga
     6541 gcataccaca tctttgtgga gttttcagac acgtcctagt taatttggtt cttttaggag
     6601 tcttttcctg ttctgtgtgt cgttgagtgt tcgcttcaat tatcgctgtg gcggattttg
     6661 aaacttttct atctatcaag gccatttagc actatatcct ttccttatat ttagataaat
     6721 acactgcatt tcaaggagtt caaaagtagt tgtattaatt ccatttgaac tctttccgtg
     6781 atataatgtg tttgtcagaa agaggtggag aaaatggcta agttacaaat ttctatggac
     6841 gaaaaattga aaaatgaagc agaaagcatt attgatgaac taggcatgag ccccaaaaca
     6901 gccgtcaccg ttttttataa gcaaattgtt gagcaaggaa aaatcccatt ttctattgaa
     6961 ttgtcggaaa aaagtaaacg aatccataat attcaaagat tagcggaaaa attgcctgtc
     7021 caaaaactag acactgacga aaaaattgaa gaatggttca atgaagatga atactaatga
     7081 tatagcaacc gtctacgttg ctttcacagc tgcagagggc ggtaaacgcc ggcctgtcta
     7141 tattctaaaa aatggactgg acacaatttc gtttctgtca attacttcaa aatatcatac
     7201 taaatccgat aaaataaagc tgaaatacgt tgaaataagg gattggaaac aggcaggact
     7261 aaaaaaagaa tcttggattg acgtaggaac tctaaacgaa ttacccaaaa acaacaaaaa
     7321 ggtcaaatgg aaaaaaattg gggaattaag tccaagcgat ttacagcgac tggaagaacg
     7381 catggaagaa ttataataat actaaaaaga agggctcttt gtcaataagg actgatgggt
     7441 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcagattt ttggctcttt
     7501 gtcaataagg actgatgagt tgtgcaaaat caaatccggg tcagaatgaa ccacattctg
     7561 gttcagattt tttgttatct tacattgatt aattgattga tcaaaaagat tctaattctc
     7621 atgttctcga atgatttaaa tccgtaggat actcgtttca ttgtctttat gtgggtattc
     7681 ttcgcttcaa tttttccatt ggaataagga tagatcattg cgctggtgat accttcttca
     7741 taggtcagaa ggttttgaag cttctcccga aagccgtcat ctaacgtttc gggaagttct
     7801 gccaataagg agaaaaataa gtcagggtct ttgtctcgaa aggcttcgac taattcatga
     7861 aaaaagggat acgcctcctt tagaggcgca gaaaactcaa gcaatcgatc aatcatcatt
     7921 gcttcagtga gaaatgggta ttttggtgct cggaaacttt tccatgtttt gtactcataa
     7981 tggttgatgc ttgcacgatt ttttagcaga aagcgccagt tctttttcag tttttctgcc
     8041 tgacttttct gtcctgcttt acgaagctca ttcatttcac ggatacgcaa ctcattgagt
     8101 gcctgattca tgtgtttgac aatatgaaac cgatcaatca ctactttcgc atttggcaga
     8161 acacgtttgg taagctggaa gtaagcggcg ttcatgtctg tgactaagaa ttctacttct
     8221 tctggattgg tgcagcctaa gaaatagctt gttaatcgag gtaatttacg cgtaggcaag
     8281 acagcaatta agtttcctgt ttcaccatct gcgcaaataa agctcatttt atcttctatg
     8341 gaagcgtgcg aacgaaattc atcaaccatc aatactctgg ggagaatctt cttagaaggc
     8401 tttggtaaat agcttttaaa ctctttcaat gtacgagtaa cggtagtcaa agatacctga
     8461 caacttttcg caataaaaga caaagatact ttttcagtca gtaaagaagc aattttatat
     8521 cgaacatgat ctgcgattga atggctgagt cggacaaaat aactttgagc cgtccaatgg
     8581 gtacgacagt ttttacaggt atagcgttgc ttttttaggc gcgtaaccaa aggcatatga
     8641 ttgtattgtt caaaacggac aatcgtttcc ttcttcccat ttttcactat aattgctttc
     8701 ccgtttccat ctaccacagt agaaccacaa cttctacaag cacgaggagt aggcgagaga
     8761 acagcatcga cgaccaacgt ctttttcttg gctctttgtc aaataggact gatagagtga
     8821 tataagaaca tctaaggtag aattgatttt gcttctataa tattttgtgt tggtggaaaa
     8881 tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt
     8941 tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg agtctctcaa tggataatca
     9001 tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag aatggctgat
     9061 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa ggaaaattgg atgactctcc
     9121 ttcctattgt gaacactgtc gatcggttcg tattatccgc aacggttcct atacgacacg
     9181 cactcagata ttgaaagtca aagaaaagtt aactatttta gaattaaaac gtacccgttt
     9241 tctctgccat gattgtggaa ggaccttttc tgcgaaaacg gacttagttg atgaacatca
     9301 tcaactaaca aaggaactga agcaggcaat tctcatggaa ctgtacgaaa atcaatcacg
     9361 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
     9421 agcaacgaag cattaccagt cacgtatgaa cttccttccg acggtgttgt gtatggatga
     9481 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
     9541 ccatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
     9601 gcactttcct agaaaagccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
     9661 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
     9721 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
     9781 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     9841 cttactcaaa gattccacga catgggagcc actcaaacgg cattatcatc gactgtttaa
     9901 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
     9961 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag gtacggaagg
    10021 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
    10081 cacgatcttc cattgctacc aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa
    10141 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
    10201 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
    10261 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    10321 gtcttcacca acacgatttg acgaagagcc tatttaaata actacatcca tttattctat
    10381 cttctaaaat agcctttttc atttcagtta aatagttcag cgccattttt gcactcgact
    10441 tgcatagtag ttttttccat attcctcaac ctcagcgggg gttgaagaca taaggaaaat
    10501 cctatgcgtt atgctcaact ggatctgatt aaatattcta atgtattttt ctctaccatc
    10561 attaaatggt taatagccaa aaatcaccac ttttcaatgg ctccttgttg tataagtata
    10621 actagaagat gaacacgtct gagaaattat agaagctttt tacggtaatt actggggctg
    10681 tgtccagtag tttttttgaa gagaacagaa aaatagctca cgtgatctat tcctactttt
    10741 tgactaattt catcaataga taagaatgtg tctttcagta gagcacttgc tcttttgatt
    10801 cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt cttttttgaa ggcccgtcct
    10861 aaataagcgg gatggtaatg gaattgtgta gctacatctt tgagcgtaag cttttcacta
    10921 taattgtttt gaacgtaatc aatcacttgt tgaattgact cattaaaaga atcatcagtt
    10981 agagagtttt gattagctgc tgatttacgc attttttctt cttgtactaa cttaactaaa
    11041 taaggaaaat caatgggttt cgttatgtaa cttttaatag ataaatcaat cccctttttt
    11101 gcatactgaa aattttggtg ggcactaatg acaatactag taaagtagcg attggatgtt
    11161 tgttgcaaag attcgattaa ttcgagtcca gaaattttcc ccatcgtaat atctgtgata
    11221 agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt cgtctccgtc tgtgtagcag
    11281 tgatcgataa tgatgtcggg gactttcttc aaaagataat gccttatgcc ttctacaatt
    11341 gtacaatcat cgtcaacaat tgcaattctc aacatgcata ttcccccctt tttgtatttt
    11401 gatagtaatc gttgttccga cgtgtagttg gctttctata acaaaacgtg tatctccagg
    11461 atagagatgc tttatacgtc gaaaagaatt tgagatgccg atattctcac tataaggaat
    11521 atcatcatta agtttttgtt gaatatgctc cagcttgtct tggtccattc ctttcccatt
    11581 ttcaacgatt tttaacacat aatggttggt tgtttcagtt gcatagacag tcaaacgatt
    11641 ttcctttttt gatgaatcga agccgtgttt aatataattt tcaacaaatg gctgtaaaga
    11701 aagttttggt aacattaatt gatcaatatg atcaatcagc tgatagtcaa agttatgtcg
    11761 ataacgacgt tgatgtagtt gcaaggagag gtcgatatta attaattcct ctttcaaatc
    11821 aattatgtcg ggctgatatg tattattgcg atacaacttg ctcaataaat atatactgtc
    11881 gctgatttct tcgtcatcat tcaatatggc agtcattcga ataaattcca aattattaaa
    11941 tagaaaatga ggctcgattt gatattgaag tgctgcatac tttgcttctt gcttcaacca
    12001 attattttca taaagtgttt tttgatatgt agcattatct aaataaaact gattgatatg
    12061 catagcgatt tctttcaaat caccgacttt gttttcgata ttgattgact tgtagtctcc
    12121 tgtagatgaa tgatttaagg aagtaatgat ttcctgatac tgctggatat agttacgaaa
    12181 acttctattt aataattgat gaatagcaag aataaacaaa ctcattaata agaaaatcat
    12241 gattgccaga cctaataagg tttgtgacga ataatttgga ttttcatata cttgtaatgt
    12301 gtggttaccg atagctatct gttgcgtttt tttccattta gaaggcgaga tgtcttcaga
    12361 taaattgacg gaatcaatac cattagacaa agaataattg taatgtgttg gaaagagact
    12421 cttttttagc gcgttgatcg aaacgtctag aatcaatgta gcatcgggca tgtcctcact
    12481 aatgaaactt aatgaacgaa taaaagaaat tttttcgttt tttgtaggtg cgtaggcact
    12541 taagtatttt tcgtaatcta atgagataga tatattttta acttcatcat cttgcacaag
    12601 tttttttaag ttatctacta aataggtagg ggagcgatcc tttgacaaaa tatcttccat
    12661 gtattcagca ggtgatgaga ggtagtagtc attaaggttt tcttggaaat cataattttg
    12721 atagatctct gtcacgatac cggagagaat acgattatat gtttccatat agtgggtgat
    12781 ttgctgaatg ttttgcaggt tttgattttt ttctttttca atcacattga tataggaaaa
    12841 gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc aatatgtatg taaaacgcat
    12901 aataactttt gataaatcgt ttttcattca aagtcctcat tttgataatc tctaatttta
    12961 tgaatcatta tagcacatat tacaagtgat gtaaacgctt tgcaaacagg gctacttatg
    13021 attgtgaata cagtttccgt ttaaaaacta ttctattgta gaggtttaat aaactaaaaa
    13081 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
    13141 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
    13201 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
    13261 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
    13321 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
    13381 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
    13441 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
    13501 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
    13561 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
    13621 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
    13681 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
    13741 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
    13801 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
    13861 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
    13921 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
    13981 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
    14041 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
    14101 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
    14161 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
    14221 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
    14281 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
    14341 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
    14401 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
    14461 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
    14521 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
    14581 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
    14641 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
    14701 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
    14761 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
    14821 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
    14881 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
    14941 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
    15001 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
    15061 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
    15121 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
    15181 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
    15241 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
    15301 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
    15361 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
    15421 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
    15481 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
    15541 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
    15601 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
    15661 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
    15721 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
    15781 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
    15841 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
    15901 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
    15961 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
    16021 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
    16081 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
    16141 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
    16201 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
    16261 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
    16321 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
    16381 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
    16441 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
    16501 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
    16561 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
    16621 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
    16681 acgatagctt tgattgctgg cgttgtgaat cgaaaaaaat atggcttgca agagtctgtg
    16741 aagttcatga gtaaaatcta taagaaaaat tttgttagat ataatctata ttgctggtca
    16801 gcaactttgt tgttggccgg caatattact acgatctggc tttgttggca gcaaagcgca
    16861 ccattgtttt atttattaag tacattatta tcggtaatta cactgttcct agtctttgca
    16921 atgttgaatt gggtcgtgat caaaagtctt gggttgtgga tgatgggaga ccgactgatt
    16981 gactatttgc ttttcacagc aacgatgttg ccaacggtag tcatgcaagg cttggtgctt
    17041 ggaatttatt tccgaatatt gatgattttg cctgtgattt ttctgtttgg cggaacagct
    17101 gtaacggtaa tattgattag taaattaact gaaagagcgt tagcaaagtt gccttgtcag
    17161 atacaatcgc actaattttt tcacaaaaag gagtatttct ttctatgaag ttaactgata
    17221 aacaagtagc taatatagcc tttatccagc aacttgagat tccattgaca caaaaagatg
    17281 aagaacaaat agacttgatt tttactgaca caaaggctaa tgtgcgacaa atcaagatta
    17341 ctaaaaatga gcagcaacga atttgcttgt cttattcaca tgaagacttg ctgtatcgag
    17401 gattgtttat ctgccttcca cgttttttag ctcaaggagg agagatgcca tacattgaag
    17461 aaacctcgat cattgagacc agttattcat tagatgcttc tcgtaatggc gtgatgaata
    17521 aagagactct aaaaaaattc atttcttact tagcgttgat ggggtacaac acattgtata
    17581 tttacacaga agatacctat gaagtagaag accttccata ttttggttac cttcgcggag
    17641 cgtatacaaa agaagaagtg aatgaaattg ttgaatttgc ttctggtttt ggcatagaag
    17701 tagttccatc gatccagacc cttgctcact tgacccaatt tttgaagtgg tatgctcaac
    17761 aagaactgat ggatgatcaa aatactctac tgatcggtga agaaaaggtt tatgcggcaa
    17821 tcgaaaagat gattattgcc tgcaaagaaa tgtatcagac agagcgtatt catttgggaa
    17881 tggatgaagc ctatcaagca ggtctgggac gatatttaag cctgtatggg tattgtgatc
    17941 ggacaacttt aatgattcaa catatggacc gtgtgttatc aattgttgat aaatataaga
    18001 tgaaaccaat gatttggagt gatttcattt ataagctatt agatgaaacc ggtaaagata
    18061 ggtattacaa tcctgatttt aaactcaaag cattagcagc taaaggatta ccagcaggat
    18121 taacctatgt tcattgggac tatggttcag atgaagtttc ctcctatgag aaagtaattg
    18181 ataaccattt gaaattttgt gacattgatc attacgtttt agctagtggt gcccatatct
    18241 gggggagact agccccgaat catgggaaaa cagaagcaat tgtttctgca gctgtaaaag
    18301 cttctaaaaa taaaggtgta cgttctgtga tgttaactac ttggggagat gaagggcagg
    18361 aaacagagca ttggcattcc ttaattgggg cattaaaatt agcagattat gtctattctg
    18421 aagaagagtg gaatgatttt gaggaaatct acgatcgttt attaggaaca ggtagttatc
    18481 aattaatgtc cgaattacga tatttcgatg aagtggatac cattacaaat aacaacttac
    18541 aaatggcttc cgtgagtaaa ctgctacttt ggcaagatcc gttagttggt atatatgact
    18601 ttcatgtagc cgctcataac aaaattgcca ctagaacaat tggtacttat tatcatgagt
    18661 tagctcagaa attagatatg aataaaaatt ggaatccaaa acaaccaatt ttaacaacta
    18721 ttcgtcaacg atatatactt ttagctgaaa ttttagcaca aaaggcagaa ctaggcatct
    18781 atttacaaca ctgctgtctg gtagaagacc ttgcaggatt aatggaatta gcgaatcggt
    18841 gtgaagcact agccaatcga gtaaatgaat tggcatctat tcatgaaata atttggtatc
    18901 aaacatacaa aacctatggt tgggaagtat tagaacaacg atatgctgca ttagctcatc
    18961 gtctgaaaac cgttgcgcgt cgtattaaaa atcatcttca ggggcaagat gccctagagg
    19021 agcttttgga agaacgatta ccgttttgtc aatatcaagt gccgcttgaa atttctggct
    19081 ttaattatcg gcaaagctcg gtgtcaggct acaattaacg aaggaatctt cccgttgaac
    19141 ggaccacttg ctcatctaat gggaaagtgg ttcgttttct attcataagt agcttaccac
    19201 taactctttt attaagaaga gaaaaaaagt aggaagtgag aaaatatgtt atcggactat
    19261 tatcaaaatc tgtcagttat aagtgtcaat acagaaccat tacgtaatta ttatattcct
    19321 tatgcaagta aagaagaagc aaaacaagag ttagaaaggg aaaaaacgag ccagtttatc
    19381 gatctcaatg gtacatggga ttttcagtat tatccttctg tccaacagtt accggaaaat
    19441 ttttttcaag aggcctttac tgaaagctat gatagaattt ctgttcctgg agtttggcaa
    19501 acagagggat ttgacttacc tcagtatatc aatgtatctt atccaattcc ttttgatccg
    19561 ccttatgtac ctaatgatac cccttgtggt gtatatcggc gtcagtttca aattgagcaa
    19621 acagatgcac gcttttactt aaatctagaa ggagcagata gtaattgttt tgtttggata
    19681 aatagaaact ttgtaggcta tgaccaagtg agtcatagta catctgaatt tgatgtgacc
    19741 aagtatttac aggttggggt taatgaaatt caaattctag tgatgaagtg gtgtgatggt
    19801 acctactttg aagatcagga taagtggcga atgagcggtc tatttcgtcc agtgtatctt
    19861 cttcaacgac caactatgca tctgacaagt tatcaactac ggacgttgac tgatcctaaa
    19921 aaaggaagcg gaacattaat ggtttctttt caacgattta ctcctattga agtatcgtta
    19981 cctatttctt acgaattatt ccatcctgat ggacgtctca tagcgaaagg ggagtgttac
    20041 tcggaacaac aaataaattt ggatgttgaa gattgccttt tttggacagc agaaacgcct
    20101 aaattatata cattactttt aacatgtaat ggtgaatatt ttaaagaaaa agttggcttt
    20161 cgagcactga cgatcaagaa tcgtaagtta ttgctgaacg gaacacctat taaattatat
    20221 ggagtcaact atcatgacgt gaatcagcaa ggcacgccag tgatgacccg agagcagtta
    20281 cgtgcagatt tgttattaat gaaaaaatta aatgtgaata ctgtacgaac ggcacattac
    20341 cctaaatcac cagattttta tgaattggcg gatgagattg gtctgtatgt aatcaatgaa
    20401 gcagatttgg agtgtcatgg tgtggttaat ctatatgata ctgaagcgaa ttatcaacta
    20461 attgctaatg atccgacatt cgaaaagccg attatggacc gagtgatccg ttctattctt
    20521 ccttatcaaa atcgaagctc tatcattatg tggtctatgg ggaatgaatc tggttatgga
    20581 gtaaattttg aaaaagctca agctttagca cggaaattgg atgattcacg gttgattcat
    20641 aatgaacgct cgattgaacc agttattgac caagtgaatg attttagtaa cttggatgtt
    20701 attagtcaaa tgtatccccc actggaagat gttcatcgtc attgtcaaag aagtgatact
    20761 gaaaaacctt tttttatgtg tgaatatagc cacgctatgg gcaacggtcc tggagatctt
    20821 aaaacgtact tcgatttgat tcaacagtat gattcctttc ttggcggttg tgtctgggag
    20881 tggactgatc atgcaatcaa tagaggaact ttagaaacgc catcttattt atacggtggt
    20941 gattttggtg aaaaatatca cgacggcaat ttttgcgtag atggtttatt gaccccagat
    21001 cggcaattaa cagtaggtgc attggagtac cgtaatattc attcgcctat tcaactgatt
    21061 gctgtagatg aggagctgca agaagtaacc ttgagcaata taactgcttt tacagccggt
    21121 tcagcattta acctttctct tgaatggtat atagatggaa aaaaaacagt ttatgaagta
    21181 atgaatcctt tcatacttga gcctggcaaa agttgttcat tgcccatcac agtacctaca
    21241 acgattcatg gtgctcatac ggttgctgtt tcgctatctg tttataaaaa tgatgagctt
    21301 tatggacagc aacagctctt tcttaaaaaa gaaaaaatga gattgccaag aactgctcat
    21361 cataatgatg caccacaaat ttcagtaaaa ggcacagagc tgataatcaa aggagaatcc
    21421 tttatttata tatatcaatt agcaaaagct gcgttcactt ctgtaaagat caatcaaaag
    21481 gaacttttgg aacgtcctac tcaattttca atttggcgtg caccaacgga taatgatcgt
    21541 aacttagtaa acaaatggaa agcagctgga tatgctgaaa tgtctttaga aatcagaaaa
    21601 aaagactgga aattcgtaga tgatcatctc ctgatcgaaa cgtcttatcg aggagttatt
    21661 ccaggattac agccaatttt tacaatgcac atacagttga aaataaatgg tgcaggcgta
    21721 atcaaccaaa aagtatcagt ggagaaaaat ccgatttttc cagatttccc tcgctttggg
    21781 atggactggc cgttgaatga tactttttcg aaggtttctt attgtggcta tgggccgtat
    21841 gaaagttatc ctgataagca ctatgctgcg atgtttggta tttatgagat ggagattgag
    21901 gaatttgatc agccatatat caaaccacaa gaatatggta accgctggca atgtgattgg
    21961 gtatcaatca aaaatcatca aggatctacc atgacggttt atggaaactc taatttttct
    22021 gctctgcctt atagtcaagc tcaattaacc gaaaagtcac accattatga attaataaaa
    22081 gaaaatagaa cttggttcaa tttaagttat aaacaaaatg gtatcggctc acacagttgc
    22141 ggtccttatt tagaagatcg tttccgtttt atggaagata cttttgactg gaatatagtg
    22201 attactttca aaggagaaaa tagatgaaag aaacaaagat caatcaagtc aaaaaaaaat
    22261 tatctcgtta tcaagaagtt gtttatcgaa accattgctt aaaatggtca aaaaataagc
    22321 tgaatgttcg ttttaatgga ttattttatc gtttcgaaca gttagaagaa gtctggaaac
    22381 cggtaacgaa tttccaatca tgtcctgtac agctcataaa tgatccaaaa gaaacatacg
    22441 atttatgtat aacacacctt tataaccagc atttgaaaaa tgacggattt tatttaaagg
    22501 ttactaatga caatcaactt acgattgaat cagcaaacct tcgagggttt caatatgcta
    22561 tggaagcctt attagagagt tttttttcgg taggagatca attattatta ccggaattga
    22621 ttctgaaaca tgaaccatca gtgaaaattc gaggaatcat tgaagggttt tatggtatcc
    22681 cctggacaca tgaaatgcgc ctctctcttt tttcatatat gaaagataac caattgaata
    22741 cttttatgta tgcccctaaa gatgatgaat tattacgtaa aaaatggcgt gaactctacg
    22801 atgcgcaaga attagacaaa tttaaggagc ttttatctgc tgctgaggct agcaatatcg
    22861 atttttggta cttaattagt cctggaaatg atattgatat tacgtgtgaa gaagacattc
    22921 aagttcttct gaaaaagtta gaacaactga ttgagcttgg cgtatttcaa tttggtctat
    22981 taatggatga tattgactat caattaaaag gtgctgctaa acgccgtttt cgagagccag
    23041 cattcgctca tgcctattta gtgaatcgag tagaggaata tcttagcact cggttgtcca
    23101 atcatgaatt agtcatttgt cctacagaat atgataatcg tcatggttct cgctatttag
    23161 caacgttaag tcaagaaata ccagaacaat taccgttctt ttggactggc ccaagcacgt
    23221 tagcggcgtc aatttctaca gaagagctgc aggaaatggc ttctgtctat caacggccga
    23281 tgctgatttg ggataatatt ccggtaaatg attatttaga ggataaagaa ctattgttca
    23341 tgagtccata tgagaatcga acaccgaatc tttctaagga gagataccag gttacaggag
    23401 tagtttcgaa tccaatggct caattagaag cttcaaagtt tacgatcaat agtatggcta
    23461 attacttatg gaactgtgag cgttttgatc ctttggaaac ttggacgtca gtcgttgaaa
    23521 aagtagttgg aagtaaacta caaccagctt atcttacctt aaccaatgct tttccaaatc
    23581 attacacaag cagtctgctt tcagatgaag aattactgct agccaaagac ccagaatggg
    23641 tcacaaaaga aatggctgct ttagtccaag cagtaaaatg gatccagcaa gaacaacata
    23701 catctagatt gagaacagaa ttggagcctt ggttacaagc aatcacagaa agttggactt
    23761 tctggcaaga atggcaatta aaaaaagatc caaaagaagc agaagaatgg ttggctaaga
    23821 aaaaaacaca ccgcatagga cgtgatttag taactgccca tttagagcaa attatcaaaa
    23881 gttagatatc ttaaaaaata aaaagcaaga aaaaggagaa caaaatggtc aagagattaa
    23941 ttagtgcaaa ctacagtgat gtcaagaaaa tgacgtcaaa agagttgaag caatccatca
    24001 aagcgagtga aggacgggtg attgtttgtg aaacggttgc taatactctt ccacaatctg
    24061 gagaaattac gaatgcagaa gttgcgtcag catttggtgc agatttgatt cttttaaatg
    24121 gtttcgattg ttatcgtcct gcagtttttg gactaccaaa tgtgacgatt gagaaggttt
    24181 ttgatctaca gcaaacgatt gaaaacccga ttcctaaatt aaaagaacta gtcggtcgtc
    24241 ctgtgggaat taatttagaa cccattgccc ctaaaacaaa tatgatgagt gagaaagtag
    24301 aaatcagcca aggaagagca agctctgttg aaacgataca aaaagcagaa gaactaggag
    24361 tcgattttat ttgctttacc ggtaatccag gcactggagt aaccaattta gaaattacta
    24421 aatccgtaaa aatggcaaaa aagtattttt cgggattgat tattgctggg aaaatgcata
    24481 gcgccggctc agaagagcca gttgtgaata aagaggcgat tatttcttat gcggaagcag
    24541 gcgcagatat tttactagtt ccttcagtag ggtctatccc aggcttttta gagcaagacc
    24601 taattgaagc ggttcggttg gcaaaaaaat atgatttgtt aacgatgtct gcaatcggta
    24661 ctagtcagga aagtgcccga ccagaaacat tgcggcaatt agccattatg aataaaagtt
    24721 gtggtgttga tttacaacat attggagatg ctggatacgg gggattggca ccagcagaaa
    24781 atattttcga aacctcggtt gcaattagag gaatgcgaca taccatcaac tgcgttagcc
    24841 gctcgatcaa tcgctaacaa aattaaataa aaaatggtta aattgattat ttaagtggga
    24901 acatacttga gatatcagct aacttctgtc actttaaaaa agggactgtg acagaagtga
    24961 aaatagacaa aaaatatccg aacaagagac taggatagca gaaaaactat cgttttgcaa
    25021 cgaggaccaa ctggaccgta ggagcaattc tcttattgtt cggatatttc gcttgttcaa
    25081 ggacttatgt ctgtagtgcc cttttttggt aggaaaaatg gtagggaacc atatcatttt
    25141 taatgaaaat caagcatttc aaaagt
//
LOCUS       DAJSBM010000032        24365 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJSBM010000032 DAJSBM010000000
VERSION     DAJSBM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.032"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            21..419
                     /locus_tag="ONT67_001564"
     CDS             21..419
                     /locus_tag="ONT67_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR2355309.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            403..1098
                     /locus_tag="ONT67_001565"
     CDS             403..1098
                     /locus_tag="ONT67_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR2355310.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1682..2569)
                     /locus_tag="ONT67_001566"
     CDS             complement(1682..2569)
                     /locus_tag="ONT67_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR2355311.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2754..3593
                     /locus_tag="ONT67_001567"
     CDS             2754..3593
                     /locus_tag="ONT67_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2355312.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3789..4763
                     /gene="sgrA"
                     /locus_tag="ONT67_001568"
     CDS             3789..4763
                     /gene="sgrA"
                     /locus_tag="ONT67_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCR2355313.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5061..5417
                     /locus_tag="ONT67_001569"
     CDS             5061..5417
                     /locus_tag="ONT67_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355314.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5420..6577
                     /locus_tag="ONT67_001570"
     CDS             5420..6577
                     /locus_tag="ONT67_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355315.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6646..7986)
                     /locus_tag="ONT67_001571"
     CDS             complement(6646..7986)
                     /locus_tag="ONT67_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2355316.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(8145..8585)
                     /locus_tag="ONT67_001572"
     CDS             complement(8145..8585)
                     /locus_tag="ONT67_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2355317.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            8745..9251
                     /locus_tag="ONT67_001573"
     CDS             8745..9251
                     /locus_tag="ONT67_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2355318.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9419..11332
                     /locus_tag="ONT67_001574"
     CDS             9419..11332
                     /locus_tag="ONT67_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2355319.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            11337..12338
                     /locus_tag="ONT67_001575"
     CDS             11337..12338
                     /locus_tag="ONT67_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2355320.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(12540..12746)
                     /locus_tag="ONT67_001576"
     CDS             complement(12540..12746)
                     /locus_tag="ONT67_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCR2355321.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(13392..14240)
                     /locus_tag="ONT67_001577"
     CDS             complement(13392..14240)
                     /locus_tag="ONT67_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2355322.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            14239..15306
                     /gene="gnd"
                     /locus_tag="ONT67_001578"
     CDS             14239..15306
                     /gene="gnd"
                     /locus_tag="ONT67_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCR2355323.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            15308..16309
                     /locus_tag="ONT67_001579"
     CDS             15308..16309
                     /locus_tag="ONT67_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCR2355324.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            16306..16590
                     /locus_tag="ONT67_001580"
     CDS             16306..16590
                     /locus_tag="ONT67_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR2355325.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            16623..17420
                     /locus_tag="ONT67_001581"
     CDS             16623..17420
                     /locus_tag="ONT67_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2355326.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            17434..18267
                     /locus_tag="ONT67_001582"
     CDS             17434..18267
                     /locus_tag="ONT67_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355327.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            18285..19142
                     /locus_tag="ONT67_001583"
     CDS             18285..19142
                     /locus_tag="ONT67_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2355328.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            19164..19673
                     /locus_tag="ONT67_001584"
     CDS             19164..19673
                     /locus_tag="ONT67_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355329.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            19710..20111
                     /locus_tag="ONT67_001585"
     CDS             19710..20111
                     /locus_tag="ONT67_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355330.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            20223..20576
                     /locus_tag="ONT67_001586"
     CDS             20223..20576
                     /locus_tag="ONT67_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355331.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            20610..21053
                     /locus_tag="ONT67_001587"
     CDS             20610..21053
                     /locus_tag="ONT67_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2355332.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            21070..22155
                     /gene="uxuA"
                     /locus_tag="ONT67_001588"
     CDS             21070..22155
                     /gene="uxuA"
                     /locus_tag="ONT67_001588"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR2355333.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            22148..22861
                     /locus_tag="ONT67_001589"
     CDS             22148..22861
                     /locus_tag="ONT67_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2355334.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(23068..23217)
                     /locus_tag="ONT67_001590"
     CDS             complement(23068..23217)
                     /locus_tag="ONT67_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355335.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            23613..23813
                     /locus_tag="ONT67_001591"
     CDS             23613..23813
                     /locus_tag="ONT67_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2355336.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         8287 a         3787 c         5067 g         7224 t
ORIGIN      
        1 ttttacgagg aggaacagag atgacgatct ttgaaacggt tgtaacagat gttggtatag
       61 aagcagaagt attcaggaaa gaaaaaataa tgattttatt tggagatgaa gcaccggaaa
      121 cattaacaga ttattgttat aagattgaat tgaatcaggt aagaaaagaa ataacttgtg
      181 atatgaagct ctgttttgat aaacaaatgt atgtaatcac agcagtagga gatgttgtcc
      241 aaactaattt gaatgaatta ggacatatca cgataaaatt tgatggatct catgagccgg
      301 aacttccagg aacaatgtat gtagaagata aggaaatgcc gcatttaaga aaggggacaa
      361 tcattaaaat cgatcaagag gatcagaatg ggggaaagct atatggaaat catgcttgat
      421 acagcaaata caaaaagtat ttattcttgt caacagtttt tgaatttaac aggcgtaaca
      481 acaaatccta caattctgaa aaaagaagga gcgtcaaact tcttctcgca tatgaaagaa
      541 atcaaaaaaa taatcggaca gattcctatc catattcaga caataggaag aacggaagaa
      601 gaaatgatag aggatgctcg agttatcata gaagaactgg gaaaagatac gtttatcaag
      661 gtgccggtga atcaagcagg ccttcaagcg atcaaaaagt tgaaaaaaga aggttatttg
      721 attaccggta cagctattta cacagaatta caagggtatt tagctattaa taatggagca
      781 gattatattg caccttatta taacaggatg caaaatcaag gcattgatgc tatgaaagtg
      841 attgattcgt tgcggactga gatcgaacgt acgcaaagac caacaaaaat attagcagct
      901 agtttcaaaa attgtgaaca agtcactaca gccattcgat ctggagctca tgcgataacg
      961 ttaggtaaag agctactaga tcaagtatta gagatgccct cgattgataa agcagtgagg
     1021 ggcttccaag aagattgggt gcaaactttc caagcagaaa cgattggtga actaaaaaag
     1081 gtcgctgatt acttctgaat caatttctcc ataaaataaa cgatagcctt ctcgaaaact
     1141 cgaggaggct atctgacgat tcaatattca ttaatttaaa gtttgggtga ctattactta
     1201 tttctcattt aaacgatgta ttccctaacc tctaatacat ccttgtaact ctgaagttac
     1261 gccagttttg aataaatttc ataaaaacaa atgaaaagtg tcgcataaaa atatctttcg
     1321 tgtttctcta gtataacaga ataagaggaa caagtccaga agggatattt ttagtctttt
     1381 ttaaaataac ttttcagtcg agtttgagtt tatgatagtt gtttttgaag cgatacggca
     1441 agtgaaaaaa tagggaagca tgaaagaatc tacttaatgg cgcattgatt catgtgatag
     1501 aaataaaaaa cttttttaaa ttatatcctc atgattgtaa caaatccagt gatttgtttt
     1561 ggtacaaata aaaaaaggcc ccgatgaagt tgtggcaaaa gtggtttgcc tcaaaaataa
     1621 gaaggaattt ccaaaaatca cttctcaaat tttgccacat ccccaattgg gtcaaatttg
     1681 tttatttgaa tatatttttt acataaggat ctttaatagt tacattgtct tttttcatca
     1741 ctttacggat tgttttatcc atgaattccg tatcaagagt ttttgcttcg gtagcaattt
     1801 tttctagctc agatttgtat ttgtctttat tagaaccttt atcctgtttt tttaccattt
     1861 ttactagata atagctttgc tgataagtcg ttggatctgt tacaggaatg atatcagaaa
     1921 cttccccgtt ttttagttta aatgctgctt tttttacttc agtaggaatc tctggattgg
     1981 ttgaatcaaa attcattttt ccatcagttt cctttaatgt tttatttttt ccataagctt
     2041 gaacaagctt ggagaaattt tccccttcat ttgcttcttt ttttgcttta tctgcttctt
     2101 ctttagtcga aaatagaatg atctgtgtac taacttctgg atagaattct ttccaagcag
     2161 tgtctagatc tttatctgtt aattttacat tggctttcaa accgtattgg aaagccagca
     2221 tctgtttaaa gagtttctta tatgtctctt ctgtttggcc aactgctttt aattgttcct
     2281 tgtagtttgg accgagtttt ttgatttgtt cattatattc ttttgttact tcctttgaag
     2341 aaatctcttt tccgtataca tcatcaaatg ctttgaagac aatcattttt tttaatagtg
     2401 tttgattatt tgatttaact tttgggttat cataaaaatc ctgaactgta agtgttccac
     2461 ctttaaacgt tgcaatcgtt tcattggact tgctacaacc tgagaatagt aaaattgcac
     2521 atgctgaaag gcacataatt agtagttgtt ttttcaaaaa atttcccatc ctttccttat
     2581 taaaaacagt ctaacagaag aattagggag acagtctttc attttcgtaa ggttgctgta
     2641 gatattctta gttcgtaaaa atctattctc tctatgatgg agaacattta catagttttc
     2701 acgagacaag aaagaaaagg gttgttaaac ttaatataaa ggagggatga aagatgatca
     2761 ggattattcg gttgattgga tctgttttaa tagtcttagt tggttttgtt ttaggaattc
     2821 ttgtcaataa tgcacttgcg gatatgttta ttggaaatag tgaatggatt ggagcaattg
     2881 ccggtacggt cggacattta gctgtttttt tactagcttt atttcttgct agtaaggtcg
     2941 aaggaaaaag aatagaagat tatggtgttt ctgttcgagg aaaagactgg gggtatttag
     3001 cgggaggtct cgttgttgga attggagtgt ttctgcttat tactagtcca ttatatttga
     3061 cgggggcgta tcggttagat tcaggaaacg caaacattgt gccgctgata acgagtttta
     3121 ttttatttat tgcagtgggg gcatcagaag agcttttatt ccgtggcttt tttcagcatc
     3181 agatgcttag atttggtccg ttaatagcaa tgattggatc tgctgcatta tttgctcttt
     3241 tgcacggatt gaatcctaac atgacatttc tggcagtttt caatatcttt ttagcaggtt
     3301 gctttttcag cgcacttatc tacagtacag gaagtttatt tacggctatt ggtgctcata
     3361 ttacttggaa ttggacacaa ggagctattt tgggtatacc agtcagcgga acgcaagaac
     3421 aaggtttttt ctccactctg atccaatcgc aaaatacaat aattactggg ggaaactttg
     3481 gggctgaagc atctatatca actacattat tcttgttagt cttgattgtt ggtttgttac
     3541 tctatgcata taaaacaaag cggatgaaga aatacactgc tgtttatcaa taaaagatga
     3601 agaaagcaag gctgatatcg gtcttgcttt tttaagtctg tcattgatta aacgaaaaag
     3661 taaacaaatt caagcgaata cacgacatct tttgttagat tttcacaact gactgtcgtg
     3721 ttctcatcat tttcatttta tataattcct ttatcaaata gaaggtaatt atatggagag
     3781 tgatagaaat gaagaaaaca gcgaccgtat tatttgttat tcttgggctg agtataattg
     3841 cagcaatgat tggaatcagt acttctgcaa atgaacgggc aaatgagaat ctttcattta
     3901 ctgtgaaaac tgatcggatt gtttatgaca tgacacaaca agtcattact attccagtca
     3961 aaccaaacaa aagcgtgaat gctagtgatg tacatgctgt tctaacatat ggttgggatg
     4021 gtaatggaag ttcagaaaaa gttattgggg aagtttattt aaaagacgta caatggaccg
     4081 ctggtattga gtatacaata atgatttctg cagaactatc gattgatgag atcaagagta
     4141 aagataaagt tgatttgatt gttttttatg atggacagat gacgatcacg gagaatttga
     4201 aaccaagcag ttggacagtt gttggaccgc caagcagtac ggatagcaca agtagtgaaa
     4261 gcagtacaga aaacacgagc ggtgaaagca gtacagagag tacgagcagt gaaagtagca
     4321 cagaaaacac gagcagcgaa agcagtacgg agagtacgag tagtgaaagt agcacaggaa
     4381 gcacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcatga
     4441 gtagtgagag tagcacagga agcacgagca gcgaaagcag cacaggaaaa gtaaataatg
     4501 aacttgattt ggtacctatc ataccaacta aggaacaaaa gagaaatgca gggagcaacc
     4561 taaaaagtgt gctaaaacag cctgattttg tttctcgtgg aattgatcat caagaaaaag
     4621 cgaaagaaac agataataga aaaagcgatt taccagaaac tggtagtaga accttgaaca
     4681 agctaattac atggatgggt gtgttactca ttttgattgt aggagcaagc tattttcgtt
     4741 ctctcttcca tcgagtgaaa taaagaataa aagcaacaga tctaaaattg aataaaacga
     4801 tagatttttg agtgaatatt tcattcgaga gtgctgtatt gcctggcgaa gttgtagctt
     4861 tgccagatct atttttgttt actaacatat ttgagaaaca ggaaataaca ggaacacaaa
     4921 tggaaaataa gagcaagaat aattgacaat gcacactgcg catggtagta tctctgaaaa
     4981 atcagtaacg aatctatttg tacgttattc atgtagctgt cggaataagt ctaaataata
     5041 gataaaaagg agtatgtact ttgtttgaag aatttattga tatcaatgaa aggcaagtat
     5101 atcaattttt gaactattgt tatgaaagag acgagaaatt atatgtggtg aaggatatcg
     5161 ctttagatct aaactacacc ctggctaaga tgaattcggt gattcaacaa gcagaatcgt
     5221 tttgcgaaag gtatccagaa tataaattat cttttctttc agaaaacaag atgataaaag
     5281 tcgagttttc ttctcagttt ttactgagta aagtttattc tatactttta gagggaacga
     5341 ttggttatat tttactggat tcactttaca aaggtactta tcaatcctta gaaaatctta
     5401 gtcaaaaaat tatataagtt tgcgtacgat tcaaagaaaa ttaagagaac ttaatactat
     5461 tttgaaaaac tatcatttat cttgcagctt gaaaaaaggt aatccgatag aaggagaaga
     5521 ataccgtatc cgatactttt ttcacttgat gtattggcag tcattcgacg aaaatcattc
     5581 tgatatatta tcaagtataa aaaataaacg tcaactatta gaaaaatttg cgaaacatgc
     5641 gccatatacg cgtagtatag atagaaagaa atggatcagc ctactaagaa tttcgttgca
     5701 acgaatgaaa caaggccact cgattacgta tgtcccagaa gagatgaaca aatttgtgca
     5761 tccattgata acaaaagaag agtttacgca ttacatacta gagccttttt ttgaagcaaa
     5821 cttctggtcg atgaatcaca tcactccaac agagcaagct tacctttatt ttatgtttgg
     5881 tgtaatgaac acctatttgg aagaagattt gttggatact ccagataaag tacaagaaag
     5941 ttggggctgg attgaatcgt ctattgtaga aaattttgaa aaaaacttca agatttgttt
     6001 tacaacttca gaaaaaaatt atctatatgt caatttggca atgatccatt tgtattcaag
     6061 ggtttttggg acaaaaaaga aggtggatgc ttttggaaaa tcagcagaag acagagattt
     6121 tcagcatata tttccagtga tgtatccaat catgcaacag ttttttagtg gggtagctcg
     6181 cagaattcca gagcttaata cttattatga aaagaacaga cggttgattt ttcaatactg
     6241 tatgttagtc agggatattt ttataaaaca cgaacaacct gtcatattct atattcaatc
     6301 aaagtatggt aaaacacagg aattatgggc aaagaaaagg atattatcta tgactgaacg
     6361 cccaataaaa tatagtgcat ccgctgatca gttacctgat attgtcatta gcgattatcc
     6421 tatcgacaaa aaggcatatg atcaaacgaa tacacagata ttttactgga atggacaacc
     6481 tacaaaaaat gattgggcgc gtctgaaagc tgaaatcata aaaatcagga atgagaaaaa
     6541 tagatccata ttggaaaaat tggatttcgt taattaagaa aagcggctct tcgtcaatga
     6601 gtgttggtga agaaattcaa tagaataatc gagatactca cacggctaga cagctatctt
     6661 gtctagccgt gttgttgttc tggctttagt cagttgggta gtgttcttag ttttcttgaa
     6721 atattttcct cgatataaaa agattcgttc tcggtagttt tgaaagtttc taaagccata
     6781 ggcaattcgt tttagtactt tgatatggtt gttcaaacat tctatagggc cgttggaata
     6841 gggaactcgc aacgctcgtt tgatttctgt tcgatatttc ttgaaggtcg tgaacactgg
     6901 ttgatagtgc tctggcaact ccttgtagtc ttcttttagg agcgcgacga attcaggaac
     6961 atcttgtctt ctgatagcgg aaagaaaaac ctggtagact tcgtagcctc gctttaagga
     7021 gtcatcataa tataatagcc gatcgactat ctccgcttct gtcagatgat ctcgaaatga
     7081 cggacgccag tagcgatgct gaccattcaa ttcccacgca ttcttttgaa gtagtctcca
     7141 gtatttcttt aagtgcttcg accgacgatc ccctttttta aaggtcttca ttacttgaat
     7201 tcggtggttg gtaaaggccc gaccgatgtg ttggctaata tgaaagcgat cggcgacgat
     7261 tcgggcattc ggaaaacact ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac
     7321 cactagacgt acttgttcac ggacttttct agggtagcgc aagaaatagt cgcgtaaagg
     7381 attgagttgc cgattttcca caatatcaat cagttgatgg gtcttgccgt ccatcatgac
     7441 aaagctcatg gctcccgaga cgtttttcac cgatttgaat tcatcgaaac acaccacttc
     7501 tggtaacggc tggttcaaag aaacggtttt aggccgaagg ctgcgaagta ttcgaatgac
     7561 agtggtcgga gaaatgtgct tcatacgtgc aatcaaagac atagaaatag gctcacttaa
     7621 tagctcaaga atggcttgtt tgactctgcg ggcaatggag cagcgacgat cgctaactga
     7681 atcttctgct aaaaaagtct ttccacagtg tttacatttg aagcgctgtt tgttaatccg
     7741 cacaatggtc ttgtagttac tgacatcatt caataagatg gatacttttt tccagcccca
     7801 tttaatgatc gtttgcttat tttgatagtg gcaatggaag cattcctctg gttgataggt
     7861 caatgtgcca tcaaaaacaa aacatgtttc gtcattaatg cgctctttgc gaaaggcatc
     7921 ttcatgaaag gtaagattta ggtctagaat atctagtaac tctttggtaa actctgaata
     7981 ggacatcttg aaacactcct ttgaatggtt ttcgtcgact ttattctaaa ggatttcaag
     8041 gtgtctgttt gcgtttatta gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac
     8101 actagatatt atagatccag aaaagcagat tgtttatgcc aaaattaaga cattgcaaaa
     8161 agattatttt ctttaaaatc gacaagatga ccagaaagca agagttcgtt gagctcttgc
     8221 tttcttttag ttttagaaag gaacatcctg attttatctt cgctaaatcc agtcaaaagt
     8281 tttgcagatt ggtcgtagat aatcggtttt ttcaataaag aaggggatgt acgaatctca
     8341 atatatagtt cactcaaact gagttcatct attctgtgaa caagattttt ataactaata
     8401 gaatgagtag agagcatttc ggtcacgccg ttgtttgtca attttagtaa gtggataaat
     8461 tcatttttgg ttagtggttg acgaattaag ttctgttcga taaacggtat atcatggacg
     8521 ataagccaac gttttgcgcg tcttgaagac gagcagccag aacctgtata caatttaatc
     8581 atcatacatt cctccaatag ctaagtattt atgaaataat aacatgaaga aatagtcgaa
     8641 aagtgccatt tagaggtgta aaataagaaa atgttgtcac gtataatgga actagctaga
     8701 taagaaatac tattaaatca agaaaaaaga aagaaggcac aagtatgtca aacacagccg
     8761 acgcaatagt agtgatcgat ttacaaaaag gggtatgttt tgattcttcg ccgatacacg
     8821 aattagaaac agtgacagca ttagtgaata aaagaatcaa aacatacgaa acgattggga
     8881 aaccagttat tttcgttcaa cacgaagatg ctcaattagt gccacaaaca aaaccatggg
     8941 agctattgtc ggaattatct attcctgagc acagttattt tgtgagaaaa acccatgcga
     9001 attcatttta taagactaat ttgagtgttt tattaactga attggactgt cattcgatcg
     9061 aattatgtgg ggcacaagca gagtattgta tagatacgac tgtgaagttt gctcatggat
     9121 taggatacca gcttatgatg tgtagaggag caacgacaac ttatgataat ccttttatga
     9181 aagcaaagga aacaatcgct ttttacgaga aaatttggga taaacgattt ctgacatttt
     9241 tggagcaata agttgtttct tcgtttaaaa aaagcagaat ggatactggt ttatccattc
     9301 tgctttatta cgtaaaatga gaaaataatc ctgttctatt tttatgggat aaaccattta
     9361 ttttttgtat aaaaaatgtt tgcgtttaca tataattctc ctagaaacat aaaaaggagt
     9421 gaatagcttg agaacaaaac aagtattaaa gggagcgttg gtcatcttat ttgctgtaag
     9481 tacaagcgtc ccgcaatgga cagtatatgg agagagcatt tcttccgaat cggaatctcg
     9541 ggaggtagaa accacagaaa ctactgatgc tggaaatgaa acgaaatcga gtacttcaca
     9601 aacagaaaca aatactgaaa caacggttga gacatctgtt actagcacag atcaagaaac
     9661 gaccgctacc acagataacc aacaaacaga agaagcaaca gctacaaaaa tgtcaaacga
     9721 cgaatcctct tctgctaaag taagtgacaa agagaatggg acagaaaatg atgatatcgg
     9781 taaaggtgaa aatttcaatg gtaaagaata tttgaattac ttcggtaaac agatgacaga
     9841 aatacttaat ggaccaacag aagacatcaa gtcaaaatta atagagttat tagtcactga
     9901 gcttgctgtt aatgattatg gtgtagataa agagattatc aaacatctga ttgctactta
     9961 tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg atgtcgatgc ttggaggtac
    10021 agatgatggg acaacgccta cagaaaacac aggcttctat ccaaatacaa aacaacttca
    10081 tcaaactgta ctaaacaaac aagatggcaa aatgatggat gtatatgcat attatgtcga
    10141 tcaaggttca gataaaacag tcatgatcca tggcggattt cgagggaatt ggaataacgg
    10201 gatcgttaca gaagaataca atgattttta taaggccggg tataatttat tatttgttga
    10261 ttccagagcg actggtaata gcggaggcga ttatgtgacc tatggacaat atgagtcaga
    10321 tgacgtcctt tactggataa atcaagaagt tcgagagcgg ccatctcaaa agattttact
    10381 ttatggtggt tcgatgggag cagctacgat gatgtctgta ttagcgaaag atattcctgt
    10441 caatgtcaaa ggaattattg aaaattgcgg atttgcatca attgatgaac aactacgttt
    10501 tacgtacagc cagacagtag cccccgcact accggatgcc ataaaaaatc agttagacat
    10561 cattggggat caagagcatg aagatttatt tatgggcctt ttgaaacaat actattttga
    10621 tcaggagatg catttggata caaaagctgc tctacctacg attggaatgt cagattcatt
    10681 gcctaaattg attatccatg gaacagcaga tgatgtagta cctgtgtcta acgcgcaaaa
    10741 attatatgag ttatcaggag ggtataaaga tcttttatta gtagaaggag cagggcatgg
    10801 aaaggcacag gaagttgacc atgctgccta cacgaaacat gtaacggact ttttgaaagt
    10861 agtgttccat gatcagataa acgtaaagta tgtggatgaa aacaataaaa gtctactcaa
    10921 agaccaggac gaaatccagc tttacggagc ttatggagaa aattatatga cagagcaaaa
    10981 gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg actgaaggga tttttaatga
    11041 gacgatacca actattattt tctattacaa aaaaatccca gtagctccac caaaaaagca
    11101 agatccgaca gagaatgcag acaataaggg aaagaatgaa cctcgagagg atcaatcaag
    11161 tgatgatacg caaccgattc agatgacagt gaaagtacaa aaaaagacag atgcaaaaaa
    11221 aagaatgttg ccgaaaactg gagaaaagaa acattcgctt ctcatttatt caggtggatt
    11281 tttatctgtt agcacgatta tcacatggat ttggcgaaga aagagagggt aaatgaatga
    11341 aatggttcaa gcgaatagtg attgcagtac cagttatcat cgtagttatt ttagtcactg
    11401 tatttttgat taatcaaatc acaccaaagc cgattagctt gcttgttaga aaacaatttg
    11461 acacaagtga aaaagaacaa gtatatgccc gaccagatga tttccagcaa aaaatcgaac
    11521 aaatcgatat aaaaaaagaa atatcctatc cttcaaagta caaaaataat aatatggata
    11581 tctacacacc gaaagtggaa aagaaaaaat taccgattct tttctggatg catggaggcg
    11641 catatgtagg aggggataaa aatgattgtc gtgattattt ggaatatcta tgttcagaca
    11701 cgcagcaaat catcgtaaat atagattatg aacgatcacc ggaagcaaaa catcctacac
    11761 cagttatcca attgaacgaa gccattcaag cagctaaaaa agagataaaa gatcaagcag
    11821 attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc acaaattagt ggagaatatt
    11881 tgttagcact tcaaaatgaa gaaattaaaa aagctgatca tcttgatccg actttggaga
    11941 acaagcagat tacgaagttt atttctttgt ctggtctgtt ggatccttct gcacttactc
    12001 aagtaagtga taaaatctct agctttcttt atgaaaaatg tggatgggct tactttgata
    12061 ataaggactt tgaacaatca gagaaggtca aaagtttagc cttggtcaga catgcggatc
    12121 gttggacgca aaacacattc ttaacggatg gaaatacaaa tacgtttacg aaacaaatgg
    12181 aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt cacaaaagta gattatgcgg
    12241 agaaaaatgc tgttttgaac catgaatatc agtttgattt cagcaataaa caagcgcaag
    12301 aaacatatca aaagttagta atgtttttca aggagtaaag tcttatgatg caaaagaggt
    12361 tgtaatctgt cacttagctc caagtcacaa ggagcaattt caataaacaa gcttttcatg
    12421 gtttatgaat attgtaactt gtgactgagg agttagagat ggcaaggggc accttttatt
    12481 cgaaatttgg gaatatcaca cgattattgt cacgatctct tttacgtaaa ttttataagt
    12541 cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc cgaagcctgc ggaggcaaga
    12601 agggacaaaa agccgagtgg taagcaaaag aatgctggtt ccactaatat tccgttgtta
    12661 ttgatataag gctgggaaca aagatgaata gcaaataaaa tcaatgcact agcgaaaaaa
    12721 agcaaagaaa tatggatagc tttcataagc agcacctcct tttttataat tgtatgataa
    12781 atagattaag aatctataaa tgtgacaagt ttgttaggat gtgaataaaa tgatcatcta
    12841 gcgaacttga aacaaagatg gctaatgata agcaattaat gattcagcaa tcagataggt
    12901 acaaagttta ccatcatacg tctagaccaa taatagaaaa tatgggaata taatagaaaa
    12961 gatttaggat acccccaaaa gaatttcccc aaaaattcga tgtatcctaa acttttttat
    13021 tttacataac aatcatatca actgtgaaat aaaattacaa atgataacct aaaatgaggc
    13081 caaaagccag tgttatcaag gaaatactta attcaccaaa atttttttta attttttaga
    13141 aaataagaaa caaaaataat aaaaaaaaga aaagataaat tttttactac tgtatactaa
    13201 ctttgttatc gcatctaaca agtaactcga ataataccaa tacaaccttc tccaaagatg
    13261 cgatgacata cgttctaacc caccgactat tgatgttctg taccaccctt gcagaaccaa
    13321 aaaagccact accaaatatt tggtagtggc tttttttgtt ttatggattt ttcgcttttt
    13381 ctgttatggt gctattcttt gatttcagaa atagtatgtc gaactttagc gatagaacga
    13441 cgtgattctt tttcgttgta aaacatcaaa atcacatata gagagtccaa aatgcttagt
    13501 tgagaaatac gggaagctaa agcttcagat cggaattctg tttcttcaga aatagaaata
    13561 aaaacgacat cgccaagttt agcaagggaa gaattagctt ggctagtaat aacgattgtt
    13621 tttgctccag tttttcttac ggtttcagca atatggattg tttcttttga ttgccctgtg
    13681 tgagaaatca aaacagcaca gtctttttcc gtcaataaag aagcttccat caattgaata
    13741 tggtaatccg tgctgtgaga agttgcgata ggggaacgca aaaatttatg ataagcgtca
    13801 gttgctacaa tctctgaacc gccaacaccg aaaaaataga cacgtttaga ttcattgata
    13861 atagtagccg ctttttttag atccttttgt tgtaacaact tttttgtgtc atttaaagtt
    13921 gaaatactag actcgaacac cttttgcgcc atagtgagct cagtatctgt tttctgaata
    13981 ttttcgtgga tagaaatagc agatagatca ttttcttgga tcaacatggc cattttaaaa
    14041 tctttaaatc cattataacc aagttttttt gtaaattgaa aaaaagtaga gtcagcaatg
    14101 cccagttcac ttgctaaatt gctaatagga ctatgactta ctttgcttgg attttctaaa
    14161 atataatcgg cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt tttgatttgt
    14221 agtcttatgg agacgctcat gagaattcct cattttcata ataagattat cattttttcc
    14281 gttctcatta atgttaaaga tacaaggaga gtaggaaaaa atcaaacaaa aaatattttt
    14341 gtaaaatcta tttacaaaaa aatttttttg tataatgagg gtataagaaa tggaggcgct
    14401 atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc tttaaactta
    14461 aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt tagtcaggca
    14521 agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac ttttgagcag
    14581 aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat gttaagaaaa
    14641 ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc ttattataaa
    14701 gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt ggattgtggg
    14761 acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg aggagataaa
    14821 gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag aggatatctg
    14881 tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg aatcgaatat
    14941 ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga gtatgctttt
    15001 gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg ctggctgatg
    15061 gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg gggaatcgtt
    15121 tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc tgttccagtt
    15181 ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga tagtttttca
    15241 gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt tgaaaagggt
    15301 gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg gaagagtctt
    15361 tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta ctgttgatca
    15421 agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca ttattgcaag
    15481 tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc tattgatttc
    15541 ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac accagacaat
    15601 cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata atataactaa
    15661 tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg acgatcgatg
    15721 ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg gtgtgattgg
    15781 tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc gctgtgatgt
    15841 tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg caaaaaaagt
    15901 agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga atttgacaga
    15961 agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa atgtttatct
    16021 atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg gtctgaaaag
    16081 cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac gtcaagatca
    16141 tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct acacaaggga
    16201 atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca gtcagcacct
    16261 tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat gaagaaaatg
    16321 gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga aggtggtatt
    16381 tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt cacccacgga
    16441 acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga aacgattcaa
    16501 tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg aatagctaca
    16561 atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa ctgaccgatc
    16621 tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct actttagcta
    16681 gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga caagcagaag
    16741 ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc caattagtga
    16801 ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca aaatggtatg
    16861 aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg aagctagcac
    16921 aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa gcaatccaag
    16981 atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt atctttgctg
    17041 ttgctggttt agttggagga ttaggagata aatacgcaag aaatgtggca gcacatgcta
    17101 tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat ggagaaaaag
    17161 tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa atagacgagt
    17221 tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg ggattatttc
    17281 cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa gagaaagtgc
    17341 atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg gaactggaag
    17401 gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt agtacaaagt
    17461 ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg aggagccacg
    17521 ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat catgggggaa
    17581 gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg ggttttttct
    17641 ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt accagtcgca
    17701 ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt cggattgctc
    17761 ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa atatacagac
    17821 aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct ttatccaacg
    17881 gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt aggggcgcca
    17941 ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat tcttgaagta
    18001 gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat cggtcgaaaa
    18061 gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa atctttagat
    18121 attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt tgtccagtcg
    18181 caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc ttttgatgac
    18241 gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct gaaataatgg
    18301 ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa gcctatctga
    18361 gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg ccatcactac
    18421 tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct ttaaaagcag
    18481 catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc ggtgtgatta
    18541 ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa gaaaaagaga
    18601 tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg gcactagcag
    18661 gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca atcgcggtac
    18721 cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc tttgggttgt
    18781 atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc cgtaatgcta
    18841 caggcctaaa gcaaaacact ggtgttcaga cagcgattga aatgctatca gttttagggt
    18901 tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca ttgaaatttg
    18961 cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg ccagggatat
    19021 tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga ttgaaagtca
    19081 cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc ggtattttat
    19141 aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg gatgagcgtt
    19201 tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat tccatttttg
    19261 ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa aatgcaggaa
    19321 gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc tattgggatg
    19381 aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa acgatttacg
    19441 acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt gctcaaaaaa
    19501 atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca gatgctgaaa
    19561 aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa gccactccat
    19621 ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca taaacaaatg
    19681 cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt cgtgattggg
    19741 aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa cagcgttttc
    19801 tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc attgcttacg
    19861 atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa gcagagaaag
    19921 ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc atgtttgatt
    19981 ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt ttgttgttca
    20041 gtttaggttg gtggggaatg atgatatata acaaaaatcc tcatcacgta ttcgatctgc
    20101 tatccgtata aacaggtatc actgtactag catttgggag ctactttttc ttattgggaa
    20161 aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca tccatattaa
    20221 cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg tttgttgatg
    20281 aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa aaagaggcta
    20341 ttctttttta tctagactct gttggggcag atacaccgct acagttttcg ggaaattatt
    20401 tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa aacgtcaatt
    20461 atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa acagatttac
    20521 gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt cgataagtaa
    20581 aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt acacatggta
    20641 agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg agccatattg
    20701 aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag gtcaaagaaa
    20761 aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta attagcgcaa
    20821 gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag aaagcagcca
    20881 cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat catcagatgc
    20941 ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa gataatttaa
    21001 ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt tagaaggaag
    21061 gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc gatcccatta
    21121 aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct gactaaaatg
    21181 cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt tgagagccaa
    21241 ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa ggcagggaca
    21301 gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt gtctcgatgt
    21361 ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa aactgatttg
    21421 tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat cgtcgatcag
    21481 atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt caaattgccg
    21541 ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta tgaaggaatg
    21601 accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat tccagtgtgc
    21661 gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga actttttggt
    21721 tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc agtagattct
    21781 ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga aaacgattta
    21841 gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag aaacgtggag
    21901 tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg ctcccttgat
    21961 atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat acgccctgat
    22021 catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta tgatcgtgca
    22081 atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa accactggga
    22141 gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt tattagaaac
    22201 tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt atgaaatgcg
    22261 taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag aacaaatggg
    22321 cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca atcaatataa
    22381 agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag aagaaatttt
    22441 atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata cacgggtaaa
    22501 agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa tcatttcagg
    22561 ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt gttcaaaaat
    22621 gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga tcgaagcagc
    22681 aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt tgcccgatca
    22741 taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata aagcagacta
    22801 tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt tgtatctcta
    22861 ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg agggaacaga
    22921 tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga acaatttaga
    22981 acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga gaagaacgag
    23041 aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt gcactgcttg
    23101 ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa cgacaaatag
    23161 attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga atgtcatagc
    23221 cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa cgaaattcca
    23281 tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa agtcaaaagt
    23341 tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt tattcaactt
    23401 tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc attatccttg
    23461 tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg taatttgagc
    23521 gatatatcta catagataaa actccttttg cctatcaaat atttattgca acgaaaaaac
    23581 gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta aaatggttca
    23641 acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta tttgcacatt
    23701 tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca gtgtcttacg
    23761 atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct taataaaaat
    23821 tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa aattcattgg
    23881 tcgttgaatg ggctgaatag tagtgtataa aacaattttg tggagcgtga agcttttatg
    23941 gtagaggaaa atacgaataa agtgatgttg ataaaaagat tcctatcttg aatatgaatc
    24001 gagatgtctt gaaaatgaat gagtttgacc aatcattatc ctatcgaaat ttacagtttc
    24061 gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa tatgttctag gaagagggcg
    24121 gcacatctaa atttttaagt aacggacagt tcaaatatca agcattgaaa gaggctatcc
    24181 tactgtttga aggaatacga atatataaaa acgacaaaga ggaaaaagta gttcattaaa
    24241 tcccttcaag tgatgaaaga atgagatcta ataaaacctg atctttcatc tgttttttta
    24301 ataacgtaat aattttttta ttttttgtgc tttcgccgtt attgtagagt gatttagcat
    24361 tctta
//
LOCUS       DAJSBM010000033        23831 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJSBM010000033 DAJSBM010000000
VERSION     DAJSBM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.033"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(56..790)
                     /locus_tag="ONT67_001592"
     CDS             complement(56..790)
                     /locus_tag="ONT67_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCR2355337.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="ONT67_001593"
     CDS             1117..1527
                     /locus_tag="ONT67_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR2355338.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="ONT67_001594"
     CDS             1520..2221
                     /locus_tag="ONT67_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCR2355339.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="ONT67_001595"
     CDS             2221..3807
                     /locus_tag="ONT67_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355340.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="ONT67_001596"
     CDS             3826..4047
                     /locus_tag="ONT67_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCR2355341.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="ONT67_001597"
     CDS             4044..4805
                     /locus_tag="ONT67_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCR2355342.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="ONT67_001598"
     CDS             5185..5694
                     /locus_tag="ONT67_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCR2355343.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="ONT67_001599"
     CDS             complement(5769..6308)
                     /locus_tag="ONT67_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCR2355344.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ONT67_001600"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="ONT67_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR2355345.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="ONT67_001601"
     CDS             7321..8172
                     /locus_tag="ONT67_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR2355346.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="ONT67_001602"
     CDS             8200..8835
                     /locus_tag="ONT67_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCR2355347.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="ONT67_001603"
     CDS             8855..9628
                     /locus_tag="ONT67_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2355348.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="ONT67_001604"
     CDS             10036..10833
                     /locus_tag="ONT67_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCR2355349.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="ONT67_001605"
     CDS             10811..11224
                     /locus_tag="ONT67_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCR2355350.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="ONT67_001606"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="ONT67_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR2355351.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="ONT67_001607"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="ONT67_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR2355352.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="ONT67_001608"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="ONT67_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCR2355353.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ONT67_001609"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="ONT67_001609"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCR2355354.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="ONT67_001610"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="ONT67_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCR2355355.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="ONT67_001611"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="ONT67_001611"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR2355356.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="ONT67_001612"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="ONT67_001612"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR2355357.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="ONT67_001613"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="ONT67_001613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR2355358.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="ONT67_001614"
     CDS             21908..22486
                     /locus_tag="ONT67_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2355359.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="ONT67_001615"
     CDS             22538..23020
                     /locus_tag="ONT67_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCR2355360.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="ONT67_001616"
     CDS             23137..23511
                     /locus_tag="ONT67_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355361.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7784 a         4285 c         5114 g         6648 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcacccg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatagaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa taatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcatagc atgacagcat
    23641 tcggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcattagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt t
//
LOCUS       DAJSBM010000034        23705 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJSBM010000034 DAJSBM010000000
VERSION     DAJSBM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.034"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            261..1922
                     /locus_tag="ONT67_001617"
     CDS             261..1922
                     /locus_tag="ONT67_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2355362.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1992..2339)
                     /locus_tag="ONT67_001618"
     CDS             complement(1992..2339)
                     /locus_tag="ONT67_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCR2355363.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2423..3364
                     /locus_tag="ONT67_001619"
     CDS             2423..3364
                     /locus_tag="ONT67_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355364.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3365..4408
                     /locus_tag="ONT67_001620"
     CDS             3365..4408
                     /locus_tag="ONT67_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355365.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4414..5484
                     /locus_tag="ONT67_001621"
     CDS             4414..5484
                     /locus_tag="ONT67_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355366.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5474..6427
                     /locus_tag="ONT67_001622"
     CDS             5474..6427
                     /locus_tag="ONT67_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2355367.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6439..7449
                     /locus_tag="ONT67_001623"
     CDS             6439..7449
                     /locus_tag="ONT67_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2355368.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7734..8555)
                     /locus_tag="ONT67_001624"
     CDS             complement(7734..8555)
                     /locus_tag="ONT67_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2355369.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8652..8930)
                     /locus_tag="ONT67_001625"
     CDS             complement(8652..8930)
                     /locus_tag="ONT67_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355370.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9198..11207
                     /gene="metG"
                     /locus_tag="ONT67_001626"
     CDS             9198..11207
                     /gene="metG"
                     /locus_tag="ONT67_001626"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR2355371.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11686..12273)
                     /locus_tag="ONT67_001627"
     CDS             complement(11686..12273)
                     /locus_tag="ONT67_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355372.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12448..13770
                     /locus_tag="ONT67_001628"
     CDS             12448..13770
                     /locus_tag="ONT67_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355373.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13925..14701
                     /locus_tag="ONT67_001629"
     CDS             13925..14701
                     /locus_tag="ONT67_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2355374.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14694..15263
                     /gene="rnmV"
                     /locus_tag="ONT67_001630"
     CDS             14694..15263
                     /gene="rnmV"
                     /locus_tag="ONT67_001630"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCR2355375.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15256..16140
                     /gene="rsmA"
                     /locus_tag="ONT67_001631"
     CDS             15256..16140
                     /gene="rsmA"
                     /locus_tag="ONT67_001631"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR2355376.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16591..17220
                     /locus_tag="ONT67_001632"
     CDS             16591..17220
                     /locus_tag="ONT67_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355377.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17344..18453)
                     /gene="ugpC"
                     /locus_tag="ONT67_001633"
     CDS             complement(17344..18453)
                     /gene="ugpC"
                     /locus_tag="ONT67_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR2355378.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18662..19084
                     /gene="mgsA"
                     /locus_tag="ONT67_001634"
     CDS             18662..19084
                     /gene="mgsA"
                     /locus_tag="ONT67_001634"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR2355379.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19407..19943
                     /locus_tag="ONT67_001635"
     CDS             19407..19943
                     /locus_tag="ONT67_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2355380.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19983..21746)
                     /locus_tag="ONT67_001636"
     CDS             complement(19983..21746)
                     /locus_tag="ONT67_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355381.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21749..23464)
                     /locus_tag="ONT67_001637"
     CDS             complement(21749..23464)
                     /locus_tag="ONT67_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355382.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7647 a         4329 c         4802 g         6927 t
ORIGIN      
        1 ctttttgttt attctttttt atctttgtat ggcaataata gacaatataa cgaaaattat
       61 ttaatggctt ttggaagacc atggaaaacc ttgtacaaag ctattttttc tgaaaaattc
      121 cttgaaacct cacgaaaaaa tcattatttt atgaaaaaga caaaaaaaag tgttgatttt
      181 ttaataatta ttcattaaac tcttaaacaa cttcagaaaa tattaattat tctgaaaatt
      241 ccaataaagg ggtacgtaaa atgaaaaaga aatttacatt tggtttggtt gcggtatgcg
      301 gccttgtact tgctggatgt tatggcggca gttcagccac aagcagtagt tcagcaaaaa
      361 gcggttcagc agatggagga ggcgtattta acttagtcgt gcctcaagaa atgcctacag
      421 ctgacttatc agttgcaaca gatacaatca gttttacagc attgaataac gtttatgaag
      481 gaatttaccg tcttgatgaa gacagcaaac cacagccggc aggagctagc gagttggcag
      541 aagtcagtga agacggatta acttacaaaa tcaaattacg agaagacgca aaatggtcaa
      601 atggtgatcc tgttacagca gcggattacg tttatggctg gcaacgaaca gtagacccag
      661 ctacagcttc tgaatatgct tacctgtatg cgccagttga aaatgcagaa gacattacag
      721 ctggaaagaa agataaatca gaactaggga tcaaagcagt tggtgactat gaattagaaa
      781 tcaaattgac gaaacaaaca ccatatttcc aatatctgct tgctttccca tcatttttcc
      841 cacaaaaaca atccgtagtg gaagaaaatg gggatgccta tgcatctgca agtgataaag
      901 ctgtttataa tggaccattc acattagaag attttgatgg accaggaaca gatacagaat
      961 ggacttataa gaaaaatgat cattactggg acaaagatac agtaaaactt tctgaaatca
     1021 aagtcagtgt agtaaaagaa tcttctactg cattgaactt attcaaagat ggccaagcag
     1081 atgacgtgat cttgtctggt gagttagcac agcaaaacgc aaacgatcca gcttatactt
     1141 ctgttaaaga agcgcggaca agctatatcg aattcaatca acgtgaaaag aattcaccat
     1201 tcaacaatgt gaacctaaga aaagctattt cttattctat cgatcgtgaa gctttagtaa
     1261 aacaagtact aggtgacgga tcagttgtct ctacaggatt gatcccatca gatatggtaa
     1321 aaagtccaac aacgaacgaa gactttgcaa aagaagctgg aaaattagtt tcttatgaca
     1381 aagataaagc aaaagaatat tgggaaaaag cgaaaaaaga attgggcgtt gattctttag
     1441 aatttgattt gatggcttct gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa
     1501 actcaatcca agaaaacttg gatggcgtaa cagtcaaacc aacacctgtt ccattctctg
     1561 tacgtcttga ccgctcaact tcgggtgatt ttgacgcagt actaggcgga tgggctgctg
     1621 actatgcaga cccaagcagc tttactgatt tgtttgtcac aggtaattca tacaaccgtg
     1681 gtcgctggag caatgcagaa tatgacgaag cagttaaagc agcttcatca acagacgctg
     1741 gcgatgaaca agcccgttgg gatgatttcc aaaaagcaat caagatcatc tctgatgata
     1801 tgggtgtcgt tccggtgtac caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag
     1861 gtatcgttca ccatgcggca ggcgcttcat gggattacaa atggacatac gttgaagaat
     1921 aatcgttaat accgtgagca tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt
     1981 ctcatattca cttatcctaa caaagaaaaa agcatgaaaa tagcagaaag agcaattagt
     2041 attccggcaa tgatcagcca aaagctgtac ataccgtagc tggcagacga gagcgaggaa
     2101 aattctggtt ttttctttcg gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga
     2161 tcgaagaaac ccgaagaaaa gacccataaa aaaccgccga taatcagaaa cggcaatgac
     2221 cataagaata gatcattaga taattgaatg agtgtaaagg aatggttgat gagatttttt
     2281 attaaaataa ccagaatcag tagaaaagct actgcgtagg ggatagattt ttttttcatt
     2341 tgattgctcc tttatttttg gttcattatc taataatgta gcgaaacatc tgtcagaagt
     2401 caaaagctag ggggaaacat tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt
     2461 tttcatgatc atcacgcttt ggttaattgc aacaatcacc tttttcttga tgcaattgct
     2521 gccgggcacg ccatatacga atcaggaacg tttgagtcct gaaacaatcg agatgctaaa
     2581 taaacaagtc ggtttggata aaccagttat cgtacaatat gggatttatc tttctaattt
     2641 gcttcaagga gattttggaa tttccttcca atttaaaaac cagccagtcg cacatttgtt
     2701 agctggaaga atcggaccat ccttgcagtt agggttacag gcaattatct ttggaacagt
     2761 cctagggacg attttaggaa cgatctctgc catgaagcaa aatacatggg cagatacatc
     2821 ttctacactc gtagcgatat taggtcgttc cattccaaac tttgtatttg ctgttttatt
     2881 acaatatatc tttgcaatca aattacaagt tttgccaatc gcaaaatggg atggctttgt
     2941 gtacacaatc ttaccaacaa ttgcgttggc catgtcgccg cttgctgact ctgctcggtt
     3001 tatccgtacg gaaatggtgg aagtactgca tagtgattat gtggaattag caaaagcaaa
     3061 aggattgagc cgttgggaaa tcgcctttaa acatggtctt cgaaatagtt tgattccatt
     3121 gatgacttta ttaggaccgt tagctgtagc attgatgacc ggatcactgg tggtagaaaa
     3181 tatctttgct attcctggta tcggggaaca attcgttaag tcaatcacaa caaatgatta
     3241 tcctacgatc atggctgtaa cgatccttta ctcatttatg ctgatttttg ttattttgat
     3301 cgtggatcta ttgtacggtc tagttgatcc aagaattcgt gtttcagaag ggagccgagg
     3361 ataaatggaa atgataccaa agcgttatga aacagttgca gatatccctg cggatgaatt
     3421 tcttccatta caaaaaaata ccgaagaaga acgtgaacag attactgcac cttctttgaa
     3481 ttttatccaa gattcatggc gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt
     3541 tttactattg gtcatcattt tgatctctgt gatcacaatc tttgtttctc ctcatgatcc
     3601 aactgcccaa aacgtggctt atatcaattt gcctcctcgt attccgggaa tcaatatcga
     3661 tggattgaat ggaaaagcaa tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa
     3721 cgtcccttca aacgtaaatt tctttttagg aacagacggt ttgggacgtg atgtattaag
     3781 ccgcttattt atgggaatga gaatctcgtt gctgatcgca tttattgctg cattactaga
     3841 tgtaacgatt ggtgtagctt acgggttggt ttctggtttg ttaggcggac gtgtcgataa
     3901 cgtgatgcaa cgttttttgg aaatcttatc aggaattcca aacctcgttg tcatgatttt
     3961 gatgttggtc gtttttgaac caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa
     4021 ctggatacca atggcacgta tcgttcgtgc acaaacaatg aaattaaaag atcaagaata
     4081 tgttttagct ggattgacat taggtgaatc aagaatgaaa attgcattca aacatatttt
     4141 gccgaatatc tctagtgtca ttattattca aatgatgttt agtattccat ctgctatttt
     4201 cttcgaagcc tttttaagct ttatcggttt aggtcttacg cctccgtcag cttcattagg
     4261 aacgatgttg agcgaagggt acaagacatt cctgtatctg ccttatctat tatggattcc
     4321 ggcagtaact ttatcggtta tcatgattgg ctttaactta cttgctgatg gattgcgtga
     4381 tgcctttgat cctaaaatga aagagtgaag acgatgacga aaattttaga agtgaaagac
     4441 ttagaaatct cttttgatac gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt
     4501 catttgaata aaggagaaac attggcgatc gttggggaat ctggaagtgg aaaatcagtg
     4561 acgaccagaa gcatcatgcg cttgctgtca agcaacgcca atatagatgc tggtcaaatc
     4621 ctctttaaag gtaacgatat cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt
     4681 aaagaaatag cgatgatttt ccaagatcca atgacctctt tagatccgac gatgcccatt
     4741 gggaagcaag tcgctgagtc ccttagaaag cataataaag tttcaaaaaa agagggacaa
     4801 aaagctgcat tagaattgct taaattagtt ggaatacccg aagctgagaa acgtatcaac
     4861 agttatccgc atcagttctc aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg
     4921 gtatgttatc cggaaatcct gattgctgat gaacctacaa cagcgcttga tgtgactatt
     4981 caagcacaga ttttggagct gctgaaagaa attcagacaa aaatcgatac atcaattatc
     5041 tttatcactc atgaccttgg tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat
     5101 ggaggaagaa tcgttgaagt tggaacatcg gaagaaattt tctacaaccc gcaacatccc
     5161 tatacttggg gactgcttgg ttcaatgccg acattagata gtgcgaacga tcgattgtat
     5221 gctattcctg gttctccacc ggatttattg aacccgccaa aaggagacgc attctatcca
     5281 agaaatgaat tcgcgatgaa aatcgatgca gaacaagaac caccattttt tgaactatca
     5341 aaaacacaca aggcagcaac ttggctactt gctccgcagg cacccaaagt gacaccgcca
     5401 gaagaaatcc aacgtcgctg ggctatcttt aaagagaaac aaaaacatac ttacggtcaa
     5461 ggaggaattg ctgatgccaa ataaactact tgaagttaaa ggattgaaac agtatttcaa
     5521 tgtaggaaaa aagaatgaag tacatgctgt aaatgacatc agctttcaca tttatgaagg
     5581 agaaacattc ggtttagttg gagaatctgg aagcggaaaa tccactactg gccgtacgat
     5641 cattcgcttg aatgaaccta ctgatggtga gatattattc gacggtcaag atataacgaa
     5701 gatcaaagat aaaaaaggtt tgacgaaatt ccggcatgat gttcagatga tctttcaaga
     5761 tccatacgct tcgttgaatc ctcgtatgaa agtcagagat atcattgccg aaggaattga
     5821 tgttaatgga ctagctaaat catctgaaga gcgtgcaaaa aaagtcgatg atctattgag
     5881 aactgttgga ttgaatccaa gccacggtac tcgttatcct catgaatttt caggtgggca
     5941 acgccagcgt attggtattg cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga
     6001 tgaaccaatt tctgcactag atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga
     6061 tctccaaaaa gaacatcaat tgacctatct atttattgca catgatttgt cgatggttaa
     6121 acatattagt gaccgaatcg gtgtaatgca taacggcttg ttattagaga tgggaacaag
     6181 tgatgaaatc tataatcacg gtgttcatcc ttatacagaa agtttgttat ctgctattcc
     6241 tctaccagat ccagaccatg agagaaaacg cagaagaatc aaatatcaac ctgaaccaga
     6301 tgacggacaa gtacgtcaac ttcgtgaaat cgcaccagaa cattttgtct atgcaactga
     6361 gcaggaagtc ccatattacg caaaaaaact aaaacgacaa aaggaatctc ttctggttgc
     6421 taactaggag ggacatagat gctaaaagct tttaaattcc gtatgtatcc gacagaagaa
     6481 caaaaacagc aattgattcg tacttttgga tgtgctcgat ttacctataa ccatttgctg
     6541 aaaaagcggc agaaaagttg gcaacaaaca ggagtagcca atttttcgct cacacccgct
     6601 acattaaaaa aagaataccc attcttaaaa gaagtagaca gtcttgcttt agcaaatgca
     6661 cagcttaatt tggatcgggc ttttcgcaat tatttcaagg gacgtgccag cttcccaaag
     6721 cttaaaacga aaaaaagtat gtggcaatct tatacgacca acaaccaaac ccgtacgatc
     6781 tatctggaaa atggctactt aaagttacca aaacaaaaag aactaatcaa gataaatcag
     6841 catcggccag ttgaaggatc gattcgctcc gctacgattt ctgctcgata taatgaagaa
     6901 ttttatgtcg cactactttg tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg
     6961 atcgggatcg cctatcatcc gaagacattg atccaaacat ctcgacctct agaagtaact
     7021 ttaccaaaat ttcaccagac agaagaaaag ctgcaacatg cacagcggaa attgaatgtg
     7081 aaagtccgct ccgctcacca tagaaagatc aggttagatc aggcaagcaa ttatcagaaa
     7141 caaaagagaa aagtaatgga tctttatctg aaacaaaaga accagcgaga agattatctt
     7201 gaacaattat ctgggaaatt ggtcaaacaa tatgactact tatttgtaga atcttttcca
     7261 aaagaagagg cacatgctga tttttctatt cacgactggc ataaattaat aacaaaattg
     7321 cgttataagt cccaatggta taataaaaaa tttcttttga tcaataccga cggagcagaa
     7381 gaaagcaatt ccgtaagaaa gagccaagta gtagaaaaaa tgggcagaca ttcggttatc
     7441 aagggataaa aaatgaatga aactgatagg agcgttaggg ttagccttgt taataaaaag
     7501 tcttaatgac ttttgtttct aggaattttt cttttgaaga aaagctcact aattatgaca
     7561 aaccaataaa acaatagcag aaaaagttcg tacgagcttt ttctgctatt gttaattttt
     7621 tattcagaaa gtttacgaat acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag
     7681 ttgtttttgc gttttttctt gaatgattag gtctgaatct tttgctcaca accttattca
     7741 tggtgccccg gcttgagctg atagattttt gaaggacggc cgattcctac aggacgttca
     7801 ccgatctcat cgaaatattg gagcatagct ttcttaaaat tggaatgatc gatcgtttta
     7861 taatcgatcc ctaagaattt agcaaatact tttcgagctt ccgtaatcgt gaaatcttta
     7921 cctaaaactt gtagaacttg cggctcgtgt tccattttat tgactacacg gttgaatgct
     7981 ttcaagatga tttgactatg gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg
     8041 actaaatcaa gcgagatagc cacttctccg ctagataaat ggatcttgtt actatgacgt
     8101 tctagtgtga accagcgtac ttccttggca tcatctccgg cgattagcgg ttcttctcca
     8161 ataaaggcta agtagctgac tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg
     8221 aatgtatgca gttgctcaat attttctttt gaaataacca caccagtttc ttcctttgtt
     8281 tctcttaaga cgctttctcc agttgactca tccttttgga caaagccacc aggcaatgcc
     8341 catgaatttc gataaggatg cccttttctt tggatcaaca aaactttcag ctgatcttct
     8401 tctttgttat agcacatcag tacgatatcg acagtcaaag agggcttgtc atattctgga
     8461 cgttcctgtt tttgatacca gttaagaaat tcttcttcac ttgctttatg ttcataatat
     8521 aatttttctt cttctttaga agcaaaacgg ttcatctgac cacctccatt gacagtaaaa
     8581 gtacttttta ccttattgta gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc
     8641 taggttatca tttaaatatg aaaaataaat ggaagtatga gattgaataa aaagaaaagc
     8701 agataaaacg gcaatacttt ataccgctct tcacgaatgg caaagaagcc acaaataacg
     8761 atatatacgt caatgataaa acgcaaaatc caagctgctg tcataaaact gttcaaatgc
     8821 ccatggaggt taaaataacc atacatacga atataaaaat agctgaatat taaatgtgct
     8881 aatgaaagaa aagcaatact aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca
     8941 gtcccttact aagatagata gtctttatta taccacgtgt gaggggagaa ataaaaagcc
     9001 gttttttagt agatatgaaa caaaacaaaa acccgcattt attcaaccta ttcatccttt
     9061 taaaggatag attgaacgta gatagaagtc gtttatccag tctttttcat tttgaaaaaa
     9121 agctaacagc atgctttttt tatggtatac tagcaaggaa acgaattcgc atcatgcgat
     9181 aaaggaggaa gagccccgtg gcagaaaagg aaacttttta tatcactacg ccaatctatt
     9241 atccaagcgg caaacttcat atcggcaact cgtatactac gattgcatgt gacgcaattg
     9301 ctcgttataa acggttgatg ggctttgatg tgttttatct cactggagtg gatgaacatg
     9361 gtcaaaaaat cgaaacaaaa gcagaagaac taggtgtaaa accgcaagaa tatgtagata
     9421 aaatggcggc tgatgtcaag aaattatgga aaacgcttga tatcagttat gataaattca
     9481 ttcgcacgac agatgattac cataaagcag ctgttcaaaa aatatttgat cgtttattag
     9541 aacagggaga tatttactta ggtgaatatg aaggctggta ctctgtttct gacgaagaat
     9601 tctttacaga aacacaactt gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg
     9661 gtaaagcgcc aagtggacac gaagtagaat tggtaaaaga agaatcatac ttcttccgca
     9721 tgagcaaata cgcagaccgt ctattggaat actatgaaga acatcccgaa ttcatccagc
     9781 cagaatctcg taaaaacgaa atgatcaaca actttatcaa accaggatta gaggatcttg
     9841 cagtttcacg aacaactttt acttggggag taccagtcaa gagtgatccg aaacatgtgg
     9901 tgtatgtatg gatcgatgca ttatccaatt atattactgc actaggatat ggctccgaag
     9961 atgaaagtct attcgaaaaa tactggcctg cagatgttca gatggtcggt aaagaaatcg
    10021 ttcgctttca tacgatctat tggccaatca tgctgatggc ccttgatttg cctctaccta
    10081 aaaaagtatt tgggcatggc tggctattga tgaaagacgg aaaaatgtct aaatcaaaag
    10141 gaaatgtcgt ttatcctgaa atgcttgtgg aacgttatgg tttagatgca ctccgttatt
    10201 atttgcttcg tgctgttccg tttgggtcag atggtgtctt tacgccagaa gattttgttt
    10261 cccgtgtcaa ttatgatctg gcaaatgatc tagggaactt attgaatcga acgattgcta
    10321 tgatcaacaa atattgtgat ggactcgttc ctgactatgc ctcacttgtc actccatttg
    10381 atagtgaact gtctacgacg gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa
    10441 aaatggaatt caatacagca ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat
    10501 acattgatga aacagaacca tgggtattag caaaagacga agaaaagaaa gctgaattga
    10561 atagtgtcat gatccattta gctgaatctt tgcgtatcgc agcgatatta cttcagccaa
    10621 tcatgacaga aacgccagag aagatcttca atcaactagg attggactct gaaacaatga
    10681 atcttgaagg attacacttt ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta
    10741 caccaatctt cccacgttta gatatggaag aagaagttgc ttttattcaa gaaaaaatgt
    10801 ctgaaggtac tcaaacaaac gaagatacag tcaaatggga tccagaagaa acagaacttg
    10861 tttcaacgaa agaaaaacaa atcaaatttg atgtatttga aaaagtagaa ctaaaagtag
    10921 cagaagtcat caactgtcaa aaagtagaag gggcagataa gttgctgcaa ttccgactag
    10981 atgctggaga cagccaagac cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc
    11041 caagcgaatt gatcgggaaa aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc
    11101 gtggacaaat cagtcaagga atgatccttt ctgcagaagc accagatggc tctttacaag
    11161 taatcgaagc accaaaatcc atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg
    11221 gtatgacact agttgtgtca tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa
    11281 ttccttctta ttacttgaag ccaatcatat aatacagaaa cagagaacta tccaaaataa
    11341 atcctagaaa aagaagtacg agcttcgctg atttttgtta atttagaata atactaatag
    11401 gaatcgtaaa aacagaaaaa aagcataatg ataaagggtc gggcagattt taattgattc
    11461 atctggtact cttttttatt gtccttacat tcataaagtt tgaatgcaat gactttatta
    11521 catctatatt tatatctaac agtagcgttg atgtgttaat tataagttct atcactttat
    11581 gttcaattag tttgtgctct tatatgtgtg attgaaaata tagataaaaa aacaatcatc
    11641 ggctttgact tcgatgattg ttttctttat tttgtaaaaa tcctatcact tgattttatg
    11701 gtaacctttt atttttgtct tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg
    11761 gatcgaaaag acataccaga aaatcgtacg tccgatacca taatgttgat agagctttgt
    11821 caacatcacg tgtaacaaga tcaaccctaa aagcaaggag ccagctataa accaatgatt
    11881 atcccaatgc atgacttcac taagtgccat cactccaaaa ggatagaaat aaaaatagat
    11941 cccgactaag gtcatccgcc agttctttgt aaatgttccg atattcaata aaaagaaaat
    12001 cgctgaaagc aaaataccga taggcggaaa aagcccaacc aaaaaggaat acaacatccc
    12061 ccacaagatc atcacaggac ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa
    12121 tattagtttt tgtgataacg ttgcaacagt cattaatcca aataaaatta atgtgaacca
    12181 atgccaatgg gactgctgtt gttctttacg ggaaaaatcg acccggttgg ataaataatc
    12241 tgctaatttc ggataagaac gcttaaaatc cataaactgt tcctccttcg tggaaaaggt
    12301 aggtttagta tacgaaaaat aaaaaagaaa tacatccgtc ttaagaggga gtttttcact
    12361 gaaagtggag aaaaaagaat ggtaaattaa aatattctca gttatactga aaattatacc
    12421 aaatttcaaa gaagaaagag gatgggaatg aaaggcaaac taaaacgaga aatcaatctg
    12481 tttggcgcat tagctaccgt tatgggaacc gtgatcggtg caggcgtctt cttcaaaact
    12541 gctgctgtta ctgctagtac tcagtctaca agtttgacat tgctagcttg gcttttgggt
    12601 ggtttcttaa cgatctgtgc tggattgaca gtggctgaat tagctacagc aatcccggaa
    12661 actggtggag ctgtaaaata tattgaagct gcgtatggaa aattgcccag ctttttatta
    12721 ggatgggcgc aaagcttgat ttactttcct gctaatattg ctgcattatc gattatcttt
    12781 gcgacacaat tgacgaactt attacaatta tctaccgatt atcttttgtt gattgcagtg
    12841 atcaccgctg tttctgtgac agggttgaat ttgttaggga caaaagtcgg tgcttctgta
    12901 caatcagtga cgttgattgt taaattaata ccaattgctg tgattgtcat atggggatta
    12961 ttgaccccag gacaaggaac tgtccagtta tttccaatcg aagctggaaa agatgttact
    13021 tttgtcgaag gattgagcag tgcgttgtta gcaacgttgt ttgcttatga cggctggtta
    13081 ggtgtcggtg caatggcagg ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt
    13141 attcttggtt tgagttttgt aacagtcgtt tatctattga tcaattttgt ttttttgaaa
    13201 actttgccga ttgatcatct tgccggtaat ttgaatgcag cttctgaagc atcagacgtc
    13261 atttttggag gaatcggtgg aaagctggtc actattggga tcttgatctc ggtatacggt
    13321 gcattgaacg gttatacact aactgggatc cgcgtgcctt atgcgatggc tttagaagac
    13381 gagctacctt tcagtaaaca gctgacaaag ctttcaaaga agtttacggt tccttatgtt
    13441 cctgcggttt tccagttagc agtagcttgt attatgatga gccttggttc tttcgacttt
    13501 ttaacagaca tgttgatttt tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc
    13561 tttattttaa gaaaaaaagc gccggaattg ccacgtccat ataaagtacc actttatcca
    13621 atcgttccga ttattgcgat tctcggtgct attttcattt taggtatgac gatgatgacg
    13681 caaacgaaat tagcattgat cggtataggt gttaccttga taggtatccc agtctattat
    13741 caaaaaaaga aaaaacgctc agaagagtaa aatatttcaa caacagacag aaaaagtacg
    13801 aactaggtcc tctagatctt cgtgcttttt ctatttcaat aaaacaagtt cttctatttt
    13861 gatttttgct acttctgcaa gtagtgctta tgtgctaaag taagaatgaa aaggagagat
    13921 aaacatgata tttgattctc atacccattt aaatgctgaa cagttcaatg aagatattcc
    13981 agaaacgatt cagcgagcac aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga
    14041 tacgccaacg attgaaaagt ctttgcaatt aagccatgac tatccaaata tatacagtat
    14101 tatcggctgg catccgacag aggctggcag ttatacgaaa gaaatcgaaa ataaattaca
    14161 agaacaattg actttaccaa aggttgtagc attaggggaa attggcttgg attatcattg
    14221 gatggaagac ccgaaagaag tccaagataa agtatttcgc agacagattg ctatcgcaaa
    14281 agaaatgaac ctaccaatca gtattcatac tagagaagca cttgaagaca cgtatcaaat
    14341 cctaaaagaa gaagatgttc gagatatcgg tggaatcatg catagtttca gtggtgatta
    14401 tgaatgggca aaacgatttc ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac
    14461 tttcaaaaaa gcattagacg tccaagaggc tgcagcaaat gtaccaatcg aacgattact
    14521 tgtggaaaca gatgcccctt atctggcacc agttcctttt agaggaaagc gaaatgaacc
    14581 aggctatact cgttatacag tagaaaagat tgctgaactt cggaatcttc cgacagaaga
    14641 agtggctgcc caaacatgga caaatgcgca ccgattattt ggtctaagcg aacatggata
    14701 agatgaagat ccaagagatt atagtcgttg aaggaaaaga cgatacaaga aggctgcggg
    14761 aagtagttga tgcagatacg atagaaacaa tagggtctgc aatcaatgaa gagatcctga
    14821 tgcaaattga acatgcccaa gagactcgtg gagtgatcgt ttttaccgat cctgattttt
    14881 caggagaaaa aataagaaaa atcattatgg aagctgttcc tcaggcaaaa catgcttttt
    14941 tgccgcgcac acaagctaaa ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg
    15001 atgctgctat tttagaagca ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg
    15061 tttctgaaat cactagacaa gaccttttag actatggttt aatagctggg gcacaagcaa
    15121 aagaaaaacg agaaaaacta ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat
    15181 tggaaaaacg tttgaagatg tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa
    15241 tagtggagga acaacatgag tgaatatcgt gaaattgcga caccctctag aacaaaagag
    15301 attttattga aacatggttt ttcatttaaa aaaagtttag gacaaaattt tctgactgaa
    15361 ccaaatattt taaggaaaat cgtagaaact gcgcaaatag atgaacatac gaacgtgatc
    15421 gaagttggac caggaatcgg tgcattgacg gaacaattag ccaagcatgc aaaacaagtc
    15481 gtagcatttg agatcgatga tcgtttgatc cctgtattgg ctgatacgat gcaaccctat
    15541 gacaatgtga ctattgtaca tcaagatatt ttaaagaccg atttgtcaac agctgttaga
    15601 gaaacatttc atgaggagct tccgttaaaa gtcgtagcga atttacctta ctatattacg
    15661 acaccgatca tgatgcattt tcttgaatca gatttggttg tggatgaact agtcgtcatg
    15721 atgcaaaaag aagtagctga tcgtatctct gctgaaccag gaacgaaagc ttatggttca
    15781 ttatcgattg ctgttcaata ttatatggaa gccagtttag cattcatcgt accaaaaaca
    15841 gtatttgtcc ctcagccaaa tgtagattca gcgattttga aactgacaag aagagatata
    15901 ccggcagtcg aagtgacaga cgaaaaagca tttttccgat tgacaaaagc ggcatttcag
    15961 caaagaagaa aaacgctttg gaataacttg cagcattcgt atggcaaaga tgaccagaca
    16021 aaagcatggt tagcaaaaag cttggaaaca gctggaatcg atccaaaacg tcgtggggag
    16081 actttgtcat tacaagaatt cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa
    16141 aaaaagaggt tgtgacagaa gttgtgaaaa agctgtcctg catcaagtaa taagaacagc
    16201 caaacaaaaa tagttcctca tgtttttcgt ttgtttgggc ttgttaccgc agatgcaagc
    16261 ttttgaacac cgtttaagaa agaggttgtg ggcctgccgt ttaactctaa gtcacaagga
    16321 acaatttcaa aaaacagctt ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa
    16381 gcttttgacc actattatgc cgaacaagga gatgtgacac aaattttgtc acatctccta
    16441 tttttgtttc ttgatcaaaa aattcttata cttgatactc acactcattt tgtatggatt
    16501 tagaaaagaa tatgtgtctt gacagttatt agtaagtgag ttatactaag tcgacattta
    16561 tgtgtcaaga catattagga gagagaaaaa atgagaaaaa attcagttcg aaaactaacg
    16621 atttctgctt tattgattgg gatgggggtc attatcccaa tggtcatgcc taaaatcatg
    16681 attccaccag cttcctttac tttggcgtct catgtgccat tatttattgc gatgtttttt
    16741 acaccaggag ttgcagtggc tgttgcgcta ggtacgacat ttggtttctt tttaacaacg
    16801 ccggttatca tcgcattgcg agcattgtca catcttgtgt ttgcgttgat tggggcttgg
    16861 tacttgcaaa agcatccgag tatcgtttta aaaaatggac agtttacttt tgctaattgg
    16921 cgtttccaag ggttcaattt tgtgatcggt gtgattcatt ctttagcaga aatgctagta
    16981 gtcagcatct tttactttat tggtaatatg cctgaaacat attattctca aggatatttt
    17041 tatacagtat tcatcttgat ggggattggt gggcttatcc acagtttagt tgattataat
    17101 attgcttatt ttatagcagg aacactcagc aaatattttg atatccccgt atttacggca
    17161 gcaaaaaaag aaaatcggaa agagtcgatt caagggtacg cgaagaaaac gacatattag
    17221 acatgatctc tcaaaaaaaa aactctcatc tagtaatgga taataaatcc tctaagatga
    17281 gagttttttg tcctaaaaag agctgcacca acatcttctg gcacagctct aacagggggc
    17341 aatttatgga agtttgatga cttgttctgt ttctttatca aagaagtggc ctttgctgat
    17401 gttgaaggca aggtcgacta tttcgcctgg tttatggaaa tctcttgcat ctacttttga
    17461 gatgaactca gtagaaccag tttttgtgta aagcatcgtt tcagctccaa gtaattcgga
    17521 aacgacaact tctgatttca ctacggcttc aggtgaagct tcaagggcta cttgttcact
    17581 gtggatgtct tctggacgaa taccgaagat aacttctttt ccttcataac ctttttcaac
    17641 taatactttg ttttttcctt caggaatacg aagcttaagc ccatgtccat ctgtgataac
    17701 accatttttc aatgtcacat taaagaagtt catggcagga gatccaataa agccggcaac
    17761 gaacatattg acgggtgtat cataaacttc tttcggtgta ccgatttgtt ggataaatcc
    17821 gtctttcatg ataacgattc ggtcagccat agtcattgct tctgtctgat cgtgagtaac
    17881 atagatggtc gttgtttcaa gacgctggtg aagtttggca atctcagcac gcatggctac
    17941 acgtaattta gcatctaaat ttgataatgg ctcatccatt aagaagactt ttgcatcccg
    18001 aacgattgcc cgtcccaatg ccacacgttg gcgttgtcca ccagataaag cagctggttt
    18061 tcgtttcaag tattctgtca gacctaagat ctctgctgca ttgtccacac gttttttgat
    18121 gtctgcttta tcatatttac gtaatttcaa gccaaaagcc atattatcaa aaacagtcat
    18181 atgaggatac aatgcgtagt tttggaaaac catggcaata tcacggtctt taggtgccac
    18241 gtcattcatg acttttccgc cgatatttag ttctccttca gagatatctt ccagtcccgc
    18301 gatcattcgc aaagtagttg actttccaca accggaagga ccaacaaaaa cgataaattc
    18361 acggtctttg attgatagat taaaatcagt tacagaataa ttttctgcat tgtcatattt
    18421 tttatatacg tttttcagag ccatttctac cataatacgt tccacacttt cttttttagt
    18481 tttattaact atttaaagta taaatgaaag cgttaactaa cttcaatgtc atgttgaaca
    18541 agaatttttt cttttttttg tcaatgtgct agttttaagc tttaataaat atccataaga
    18601 aataaaaact gatacactgg agtaaataag tatgttaaca atatcaaaag gagtgtctct
    18661 tatgaaaatc gctttgatcg ctcatgaccg aaaaaaacct atgatggtga aattagcaac
    18721 agcgtataaa acaatattgg aaaaacatga actttttgct accgggacga ctgggcagag
    18781 gatcacggaa gcaaccggct tgccgatcca tcggtttaaa tccggaccgt taggcgggga
    18841 tcagcaaatt ggagccatga tttcggagga tcagttagat ttggtgattt ttcttcgaga
    18901 cccgctggct gcacagcccc atgaaccaga tgtaacagca cttatccgtt tatctgatgt
    18961 ctatgagatt ccgttagcta cgaatatcgg aacagctgag gtgctactaa gaggactgga
    19021 agccggcttc ctagattttc gaagaatcgt ccatgaaata gacaagcatc cactttcatt
    19081 ttgatggata agcgtatgga aaaataacgt ttaatgttcg cctatatgta gctataaaag
    19141 ctaaacaaac tgataatatg cgatataata cgaacgtatt ggataatata aacaataata
    19201 ttagtttttc gtatttgcag taaatgtgaa aatatgctat agtttctgtg ggaaaagatc
    19261 ccaaaaataa tacagagtag aaaataaagc gttaagtgct aagaggatgg gatgttgcct
    19321 cttggacgaa ggacgtaatg ttcgcggttt tattttcgca ttcgctgcat gatacgatgt
    19381 agcagctctt aaaggagatg ctaaaaatga agaaacaccg gttagacgca cgtatgattg
    19441 ctattatggg cttattgatt gcattgatgg tcactttgtc ccgactggta gcaatcgaga
    19501 ccccatttat taaaatcagt gtcacattta ttccacaagt gatcatgggc attctttttg
    19561 gtcctttttg gtcagggata ggcgcagttt tagctgattt agtaggaatg gccctcttct
    19621 cgaaatctgc attctttatt ggatttacat tgaatgcatt tattgaagga gcaatctacg
    19681 gattcttttt ctatcgaaaa gagatcactt ggaaaaatgc cattctggct acgcttagtg
    19741 tcacgctgat catcaactta tttttgacac cattatggct tgcgttgatg taccatgtgc
    19801 cattgttcag ctgggtcgtt tgggcgcccc gcctgttaaa aacagtaata tggttaccga
    19861 ttcaatcaat tgccatttat tacgtaggac gtagtattcc ttacaaaaaa attttaagaa
    19921 gtttagcgat ccacgctaaa taaaaaacaa gcagtcttcc catcccagaa gactgcttgt
    19981 ttttattgtg ctgtgaattg tgcttgatag agccgtcggt aataaccatc tttttgaagc
    20041 agctcatcat gggtgcccat ttcgacaata gctccctgat ccatcactaa tatctggtcc
    20101 gcatttttga ttgtggacag gcgatgggca atcacaaaac tggtttttcc ttccatcatt
    20161 gctaagaagg cttgttggat ttttttctct gtcaatgtat cgactgagct ggtagcttca
    20221 tctaagatca acataggggg attactgatc atcgttcgag caatcgtcaa cagttggcgt
    20281 tgtccatctg atatctttaa cccttgaccg ccaatcttag tatctaattt ttccggcaaa
    20341 cgttctacaa attcatacat ataagaggct tttagggcat cattgatttc tgcgtcactt
    20401 gcttccggat tcccaaaacg aagattttct cgcaatgtac tgtcaaatag ccaagtatct
    20461 tgtaacacca ttccaaaact tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg
    20521 tcatcgactg taattgttcc ttttgttgtg tcataaaaac gcatcagcaa attaacaagt
    20581 gtggattttc cggcccctgt ttttcctact atagcaatgg tttgacctgg ataagcagta
    20641 aaattgaaat cttcgatcaa acgctgcttg gggtcataag aaaaagaaac atgatcaaaa
    20701 gagacttttc ctttgatatt tttgggttcc aaggcatagg catctggcaa atcaggcttt
    20761 tcttctggct gatcgatcaa atcaaatgct cgttctagac cagaaaaagc ggtttgcagt
    20821 tgtgtcgtga ttccagaaag ttcgataaat ggtttagaaa actgacttga gtagatcgta
    20881 aagctggaaa tcacaccaac agtgattgac gaaccactct tcaatgccaa taggccgcca
    20941 actaaaccaa tcgataaata agcaaggtga tctacaaaac gagaagacgg gtttgtcaag
    21001 gaagaagaaa attgtgcccg ttgtcctttt tcatatagat cagcatttag cagttcaaat
    21061 tgctgttgac tagcttcttc ttgctgaaaa gctttcacga ttttctggtt accgattcgt
    21121 tcggtcacaa agcccgagat atccccaata atcctttgct gtctatcgaa atttgtttgt
    21181 gaagcacgag caacgatcca actcacaagg aaaataattg gtgtagaaca aagaacgacc
    21241 aatgtcagaa gtggacttag acgcatcata aaaataaacg aaagaatgat gattgccact
    21301 ccagaaaata attgattgaa ggccgcagaa attgcaatgg aaatattgtc catatcattt
    21361 gtaaatcgac tgacgatatt tccgtgggag ttttgatcat aataacgaag tggcaatcga
    21421 ttcagatgag caaatgcatc ttttcttaat tttgcaacgg ataagtaagc taaccgattg
    21481 cttaacactt ggatgagcca ttgactgact acgttgatta aaacgatacc accgaataga
    21541 ataagtaaat ggaacagtcg attgaagcgg acctgtcctt ctccaatcat ttgatcgata
    21601 ctttgaccaa tttgtaacgt catcacaact gttgcaatcc cattgatgat tcctagtatc
    21661 acagctagcc aaatgtcttt tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc
    21721 tgcaaggata atttttttac tttcataact attcttcctc ctgtgatgcg acgatttcct
    21781 gatattctgc tgattgagca agtaattctt catgggtacc ctgagcagca atcttacctt
    21841 gatccatcac taagatatga tgtgcttctt gaatagagcg gacacgttgg gagataataa
    21901 tcactgtact gccaagataa gtctgtagcg ccttacgcaa atccaagtct gtttgataat
    21961 ccaacgcact taaagagtca tctaaaatca gtaatgctgg cttggcaatc aaagctcttg
    22021 caatcgtcag tcgctgtttt tgtccgccag agaaattttt cccattttcc atgacttttg
    22081 tatctaaacc atttggaagc tgacggacaa attcttctgc ctgagcgatt ttcagagctt
    22141 cccaacattc tttttctgtg gcgtctttct taccccattg aaggttttca cgaatagtcc
    22201 cggtaaatag tacagaagtt tggggaacaa gagcaatttg ctgacggaga tgatccagct
    22261 gccaagaacg aacatccatt ccgtccatta taactgtacc ggaactgaca tcatagaaac
    22321 gcggaatcaa ttgtgtcaaa gtcgttttcc cgcttccggt tggaccaatg attccaagga
    22381 ctgtatttcg cttcagctga aatgtaatat tttggagaga aagaccagaa tcaggagtat
    22441 aacggaagtc aacatggtca aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac
    22501 tgacctcagc ttcaagaatc gaattttctg tgtctagcac ctctttcacc cgatttcctg
    22561 aagcttcggc acgtgtgaag atcacgacga ggtttgaaac aacgatcagt gccaaaagca
    22621 tttgattcat ataattgatc aaagcaagaa cttgtccttg ttgcaagcta ccaatattta
    22681 ctttgatccc gccaactgtc agcaaacaga tgatcccgac attcatgatc aaggttgtag
    22741 caggtgataa tagggcagag atattggaaa cacgtaaata attgttcgct agatcatttg
    22801 tgcttttatc gaacttctct gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa
    22861 caccgctaag attctgacta acgtgttcat tgagctgatc tagtttttct tgcacttttt
    22921 ggtagagagg aacggttttt ttgatgataa aaaataaaat gattgagaaa ataggcaata
    22981 atgctaagaa aaacagacct acttcccagt cgatcacaaa agccatgacc actgacccga
    23041 tacttaaaaa cggtgctctg atcaataaac gaatgaccat tgctaaagct agctgaagct
    23101 gattgatgtc gtttgtcatt cgggtgatca aggtatctgt tccaaaatga ttcaactcgt
    23161 tgtgagagaa agtattgatt tttttgatta gctgattacg taactctgta ccaaatcctt
    23221 gtgaagctac tgaagcatag tattggcaaa tgatagcaca aatcagacca atcacagaca
    23281 tagcgaccat ccataagccc atttcaatga tttttcctcg gtcattcatt tttagtccat
    23341 tatcgatcaa ggaagccatc attaaaggca ggacaagttc gaagacagct tcaagaaatt
    23401 tgaagaacgg gcccaaaatg atttgcttac gatagtcttt tgcataacgt aataactgaa
    23461 tcatatgatt cctcctatta tttatttgtc cacaattgaa aaaacaagta ctaccacatt
    23521 gtagcttagg aaatttagaa tgaaaagaga acaagccttt tcataaatga aagttttatt
    23581 atgaagaagc tttagaaatt gaatagacta ttttttttaa aaaaaagaaa atgagaaaac
    23641 atgccagtct cttcattcat aggaacgtct attttactta atttttctat aaatgaattc
    23701 tgcat
//
LOCUS       DAJSBM010000035        23387 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJSBM010000035 DAJSBM010000000
VERSION     DAJSBM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.035"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            2..74
                     /locus_tag="ONT67_001638"
     tRNA            2..74
                     /locus_tag="ONT67_001638"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ONT67_001639"
     tRNA            78..150
                     /locus_tag="ONT67_001639"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ONT67_001640"
     tRNA            155..225
                     /locus_tag="ONT67_001640"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ONT67_001641"
     tRNA            238..311
                     /locus_tag="ONT67_001641"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ONT67_001642"
     tRNA            323..394
                     /locus_tag="ONT67_001642"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="ONT67_001643"
     CDS             660..1853
                     /locus_tag="ONT67_001643"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR2355383.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ONT67_001644"
     CDS             1983..3455
                     /locus_tag="ONT67_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355384.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ONT67_001645"
     CDS             3538..4326
                     /locus_tag="ONT67_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR2355385.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ONT67_001646"
     CDS             complement(4393..5061)
                     /locus_tag="ONT67_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCR2355386.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ONT67_001647"
     CDS             5176..6888
                     /locus_tag="ONT67_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355387.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="ONT67_001648"
     CDS             6891..8660
                     /locus_tag="ONT67_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355388.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="ONT67_001649"
     CDS             complement(8865..9431)
                     /locus_tag="ONT67_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2355389.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="ONT67_001650"
     CDS             complement(9431..10387)
                     /locus_tag="ONT67_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR2355390.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="ONT67_001651"
     CDS             complement(10387..11043)
                     /locus_tag="ONT67_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR2355391.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="ONT67_001652"
     CDS             11413..13431
                     /locus_tag="ONT67_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2355392.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="ONT67_001653"
     CDS             13858..16272
                     /locus_tag="ONT67_001653"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR2355393.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="ONT67_001654"
     CDS             16620..18173
                     /locus_tag="ONT67_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCR2355394.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="ONT67_001655"
     CDS             complement(18554..20338)
                     /locus_tag="ONT67_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355395.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="ONT67_001656"
     CDS             complement(20353..22074)
                     /locus_tag="ONT67_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355396.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="ONT67_001657"
     CDS             22174..22935
                     /locus_tag="ONT67_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2355397.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7392 a         4199 c         4858 g         6938 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctc
//
LOCUS       DAJSBM010000036        23296 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJSBM010000036 DAJSBM010000000
VERSION     DAJSBM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.036"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            438..992
                     /locus_tag="ONT67_001658"
     CDS             438..992
                     /locus_tag="ONT67_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2355398.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="ONT67_001659"
     CDS             complement(1165..1638)
                     /locus_tag="ONT67_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2355399.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="ONT67_001660"
     CDS             1650..2186
                     /locus_tag="ONT67_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2355400.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="ONT67_001661"
     CDS             2226..2597
                     /locus_tag="ONT67_001661"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2355401.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="ONT67_001662"
     CDS             2881..4152
                     /locus_tag="ONT67_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR2355402.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="ONT67_001663"
     CDS             4397..5422
                     /locus_tag="ONT67_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355403.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="ONT67_001664"
     CDS             5716..6825
                     /locus_tag="ONT67_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR2355404.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="ONT67_001665"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="ONT67_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="ONT67_001666"
     CDS             7820..8644
                     /locus_tag="ONT67_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355405.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="ONT67_001667"
     CDS             8676..9986
                     /locus_tag="ONT67_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCR2355406.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="ONT67_001668"
     CDS             10134..11393
                     /locus_tag="ONT67_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2355407.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEYRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="ONT67_001669"
     CDS             11403..12275
                     /locus_tag="ONT67_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355408.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="ONT67_001670"
     CDS             12287..13117
                     /locus_tag="ONT67_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355409.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="ONT67_001671"
     CDS             13157..15340
                     /locus_tag="ONT67_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR2355410.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="ONT67_001672"
     CDS             15401..16621
                     /locus_tag="ONT67_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCR2355411.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="ONT67_001673"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="ONT67_001673"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR2355412.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="ONT67_001674"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="ONT67_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="ONT67_001675"
     CDS             19583..19792
                     /locus_tag="ONT67_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2355413.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="ONT67_001676"
     CDS             19785..19934
                     /locus_tag="ONT67_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR2355414.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20088..20753
                     /locus_tag="ONT67_001677"
     CDS             20088..20753
                     /locus_tag="ONT67_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2355415.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ONT67_001678"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="ONT67_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCR2355416.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="ONT67_001679"
     CDS             22456..22689
                     /locus_tag="ONT67_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCR2355417.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="ONT67_001680"
     CDS             22686..23093
                     /locus_tag="ONT67_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2355418.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7975 a         3476 c         4436 g         7409 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgagtatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaa
//
LOCUS       DAJSBM010000037        23076 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJSBM010000037 DAJSBM010000000
VERSION     DAJSBM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.037"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..2542
                     /locus_tag="ONT67_001681"
     CDS             101..2542
                     /locus_tag="ONT67_001681"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCR2355419.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="ONT67_001682"
     CDS             complement(2872..3576)
                     /locus_tag="ONT67_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355420.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="ONT67_001683"
     CDS             complement(3763..4008)
                     /locus_tag="ONT67_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355421.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="ONT67_001684"
     CDS             complement(4025..4876)
                     /locus_tag="ONT67_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR2355422.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="ONT67_001685"
     CDS             5042..6046
                     /locus_tag="ONT67_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCR2355423.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="ONT67_001686"
     CDS             complement(6287..6643)
                     /locus_tag="ONT67_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2355424.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="ONT67_001687"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="ONT67_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR2355425.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="ONT67_001688"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="ONT67_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCR2355426.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11764)
                     /locus_tag="ONT67_001689"
     CDS             complement(11003..11764)
                     /locus_tag="ONT67_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2355427.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="ONT67_001690"
     CDS             12042..12563
                     /locus_tag="ONT67_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCR2355428.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="ONT67_001691"
     CDS             12622..13482
                     /locus_tag="ONT67_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCR2355429.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13667..15982
                     /locus_tag="ONT67_001692"
     CDS             13667..15982
                     /locus_tag="ONT67_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2355430.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="ONT67_001693"
     CDS             16164..18005
                     /locus_tag="ONT67_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR2355431.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="ONT67_001694"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="ONT67_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR2355432.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19358..21457
                     /locus_tag="ONT67_001695"
     CDS             19358..21457
                     /locus_tag="ONT67_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCR2355433.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21653..22516
                     /locus_tag="ONT67_001696"
     CDS             21653..22516
                     /locus_tag="ONT67_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2355434.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22621..>23076
                     /locus_tag="ONT67_001697"
     CDS             22621..>23076
                     /locus_tag="ONT67_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2355435.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         7771 a         4038 c         4810 g         6457 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaaa
    19201 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
    19261 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
    19321 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
    19381 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
    19441 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
    19501 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
    19561 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
    19621 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
    19681 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
    19741 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
    19801 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
    19861 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
    19921 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
    19981 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
    20041 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
    20101 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
    20161 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
    20221 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
    20281 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
    20341 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
    20401 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
    20461 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
    20521 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
    20581 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
    20641 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
    20701 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
    20761 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
    20821 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
    20881 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
    20941 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
    21001 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
    21061 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
    21121 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
    21181 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
    21241 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
    21301 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
    21361 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
    21421 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
    21481 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
    21541 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
    21601 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
    21661 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
    21721 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
    21781 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
    21841 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
    21901 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
    21961 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
    22021 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
    22081 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
    22141 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
    22201 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
    22261 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
    22321 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
    22381 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
    22441 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
    22501 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
    22561 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
    22621 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
    22681 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
    22741 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
    22801 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
    22861 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
    22921 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
    22981 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
    23041 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DAJSBM010000038        23065 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJSBM010000038 DAJSBM010000000
VERSION     DAJSBM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.038"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..564)
                     /locus_tag="ONT67_001698"
     CDS             complement(109..564)
                     /locus_tag="ONT67_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355436.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="ONT67_001699"
     CDS             complement(718..1926)
                     /locus_tag="ONT67_001699"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCR2355437.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="ONT67_001700"
     CDS             complement(2038..2370)
                     /locus_tag="ONT67_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCR2355438.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="ONT67_001701"
     CDS             2518..3393
                     /locus_tag="ONT67_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2355439.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="ONT67_001702"
     CDS             complement(3475..4056)
                     /locus_tag="ONT67_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCR2355440.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="ONT67_001703"
     CDS             complement(4062..5321)
                     /locus_tag="ONT67_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR2355441.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="ONT67_001704"
     CDS             complement(5323..6369)
                     /locus_tag="ONT67_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355442.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="ONT67_001705"
     CDS             complement(6604..6879)
                     /locus_tag="ONT67_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCR2355443.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT67_001706"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="ONT67_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR2355444.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT67_001707"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="ONT67_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR2355445.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="ONT67_001708"
     CDS             complement(9978..10658)
                     /locus_tag="ONT67_001708"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR2355446.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="ONT67_001709"
     CDS             complement(10707..11342)
                     /locus_tag="ONT67_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2355447.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="ONT67_001710"
     CDS             complement(11408..12811)
                     /locus_tag="ONT67_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR2355448.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="ONT67_001711"
     CDS             complement(12798..13829)
                     /locus_tag="ONT67_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355449.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="ONT67_001712"
     CDS             13860..14081
                     /locus_tag="ONT67_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCR2355450.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="ONT67_001713"
     CDS             complement(14246..14983)
                     /locus_tag="ONT67_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCR2355451.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="ONT67_001714"
     CDS             complement(15136..16476)
                     /locus_tag="ONT67_001714"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCR2355452.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="ONT67_001715"
     CDS             complement(16713..18236)
                     /locus_tag="ONT67_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCR2355453.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="ONT67_001716"
     CDS             complement(18614..19225)
                     /locus_tag="ONT67_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2355454.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="ONT67_001717"
     CDS             complement(19555..20271)
                     /locus_tag="ONT67_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCR2355455.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT67_001718"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="ONT67_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCR2355456.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="ONT67_001719"
     CDS             complement(20830..21627)
                     /locus_tag="ONT67_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCR2355457.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="ONT67_001720"
     CDS             complement(21597..21968)
                     /locus_tag="ONT67_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2355458.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT67_001721"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="ONT67_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR2355459.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6467 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DAJSBM010000039        22462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJSBM010000039 DAJSBM010000000
VERSION     DAJSBM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.039"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT67_001722"
     CDS             <1..395
                     /locus_tag="ONT67_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355460.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="ONT67_001723"
     CDS             398..1147
                     /locus_tag="ONT67_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355461.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="ONT67_001724"
     CDS             1159..1428
                     /locus_tag="ONT67_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355462.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="ONT67_001725"
     CDS             1433..1597
                     /locus_tag="ONT67_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355463.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="ONT67_001726"
     CDS             1590..1892
                     /locus_tag="ONT67_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355464.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="ONT67_001727"
     CDS             1889..2050
                     /locus_tag="ONT67_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2355465.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="ONT67_001728"
     CDS             2047..2352
                     /locus_tag="ONT67_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355466.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="ONT67_001729"
     CDS             2352..2708
                     /locus_tag="ONT67_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCR2355467.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="ONT67_001730"
     CDS             2695..2913
                     /locus_tag="ONT67_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355468.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="ONT67_001731"
     CDS             2910..3329
                     /locus_tag="ONT67_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2355469.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="ONT67_001732"
     CDS             3326..3883
                     /locus_tag="ONT67_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2355470.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="ONT67_001733"
     CDS             3880..4176
                     /locus_tag="ONT67_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355471.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="ONT67_001734"
     CDS             4253..4666
                     /locus_tag="ONT67_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2355472.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="ONT67_001735"
     CDS             5012..5386
                     /locus_tag="ONT67_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355473.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="ONT67_001736"
     CDS             5423..5590
                     /locus_tag="ONT67_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355474.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="ONT67_001737"
     CDS             5616..5960
                     /locus_tag="ONT67_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2355475.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="ONT67_001738"
     CDS             5965..6246
                     /locus_tag="ONT67_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355476.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="ONT67_001739"
     CDS             6349..6663
                     /locus_tag="ONT67_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR2355477.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="ONT67_001740"
     CDS             6641..8335
                     /locus_tag="ONT67_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2355478.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="ONT67_001741"
     CDS             8355..9533
                     /locus_tag="ONT67_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2355479.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="ONT67_001742"
     CDS             9496..10182
                     /locus_tag="ONT67_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2355480.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="ONT67_001743"
     CDS             10182..11342
                     /locus_tag="ONT67_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2355481.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="ONT67_001744"
     CDS             11352..12227
                     /locus_tag="ONT67_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355482.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="ONT67_001745"
     CDS             12224..12535
                     /locus_tag="ONT67_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355483.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="ONT67_001746"
     CDS             12525..12878
                     /locus_tag="ONT67_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR2355484.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="ONT67_001747"
     CDS             12868..13269
                     /locus_tag="ONT67_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355485.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="ONT67_001748"
     CDS             13262..13666
                     /locus_tag="ONT67_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355486.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="ONT67_001749"
     CDS             13678..14286
                     /locus_tag="ONT67_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2355487.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="ONT67_001750"
     CDS             14306..14668
                     /locus_tag="ONT67_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355488.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="ONT67_001751"
     CDS             14671..14853
                     /locus_tag="ONT67_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355489.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="ONT67_001752"
     CDS             14870..18289
                     /locus_tag="ONT67_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2355490.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="ONT67_001753"
     CDS             18340..19077
                     /locus_tag="ONT67_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2355491.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="ONT67_001754"
     CDS             19087..21378
                     /locus_tag="ONT67_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2355492.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>22462
                     /locus_tag="ONT67_001755"
     CDS             21402..>22462
                     /locus_tag="ONT67_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355493.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGST"
BASE COUNT         8103 a         3317 c         4804 g         6238 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcgact tcgggggata tgattatagc ggtaagccga
    22021 atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg tcactaactt
    22081 cggataacga aaagctacat tttacttctg atggtacagg aagcattatt atgtttacgc
    22141 gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg aaagttcgat
    22201 ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt acatcaccag
    22261 gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat gtagggaaag
    22321 aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta gatcgatttt
    22381 atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag ttgtacgaca
    22441 tcaaaatcga agaaggctca ac
//
LOCUS       DAJSBM010000040        22028 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJSBM010000040 DAJSBM010000000
VERSION     DAJSBM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.040"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..1371)
                     /locus_tag="ONT67_001756"
     CDS             complement(61..1371)
                     /locus_tag="ONT67_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2355494.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ONT67_001757"
     CDS             complement(1383..2801)
                     /locus_tag="ONT67_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355495.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="ONT67_001758"
     CDS             complement(3041..4609)
                     /locus_tag="ONT67_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCR2355496.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="ONT67_001759"
     CDS             complement(4613..4894)
                     /locus_tag="ONT67_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2355497.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="ONT67_001760"
     CDS             complement(4891..5814)
                     /locus_tag="ONT67_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCR2355498.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="ONT67_001761"
     CDS             complement(5811..6689)
                     /locus_tag="ONT67_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR2355499.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="ONT67_001762"
     CDS             complement(6676..8013)
                     /locus_tag="ONT67_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCR2355500.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="ONT67_001763"
     CDS             complement(8016..9119)
                     /locus_tag="ONT67_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCR2355501.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="ONT67_001764"
     CDS             complement(9193..10701)
                     /locus_tag="ONT67_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR2355502.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="ONT67_001765"
     CDS             complement(10667..11359)
                     /locus_tag="ONT67_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2355503.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="ONT67_001766"
     CDS             complement(11381..12355)
                     /locus_tag="ONT67_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355504.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="ONT67_001767"
     CDS             12655..13527
                     /locus_tag="ONT67_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355505.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="ONT67_001768"
     CDS             complement(14109..16307)
                     /locus_tag="ONT67_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355506.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="ONT67_001769"
     CDS             16539..17531
                     /locus_tag="ONT67_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355507.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="ONT67_001770"
     CDS             17591..19012
                     /locus_tag="ONT67_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR2355508.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="ONT67_001771"
     CDS             19005..20504
                     /locus_tag="ONT67_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR2355509.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="ONT67_001772"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="ONT67_001772"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR2355510.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DAJSBM010000041        21910 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJSBM010000041 DAJSBM010000000
VERSION     DAJSBM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.041"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT67_001773"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ONT67_001773"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR2355511.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ONT67_001774"
     CDS             complement(1172..1867)
                     /locus_tag="ONT67_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCR2355512.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ONT67_001775"
     CDS             complement(1880..2596)
                     /locus_tag="ONT67_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCR2355513.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ONT67_001776"
     CDS             2926..3534
                     /locus_tag="ONT67_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCR2355514.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ONT67_001777"
     CDS             3859..4182
                     /locus_tag="ONT67_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCR2355515.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ONT67_001778"
     CDS             4364..5734
                     /locus_tag="ONT67_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCR2355516.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ONT67_001779"
     CDS             complement(5864..7165)
                     /locus_tag="ONT67_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2355517.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="ONT67_001780"
     CDS             complement(7519..9477)
                     /locus_tag="ONT67_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCR2355518.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ONT67_001781"
     CDS             complement(9654..9920)
                     /locus_tag="ONT67_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2355519.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT67_001782"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ONT67_001782"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR2355520.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT67_001783"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ONT67_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR2355521.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="ONT67_001784"
     CDS             13264..13827
                     /locus_tag="ONT67_001784"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR2355522.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ONT67_001785"
     CDS             complement(14080..14601)
                     /locus_tag="ONT67_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCR2355523.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ONT67_001786"
     CDS             complement(14604..15446)
                     /locus_tag="ONT67_001786"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCR2355524.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ONT67_001787"
     CDS             complement(15439..16164)
                     /locus_tag="ONT67_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR2355525.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ONT67_001788"
     CDS             16340..16636
                     /locus_tag="ONT67_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355526.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="ONT67_001789"
     CDS             17204..18796
                     /locus_tag="ONT67_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR2355527.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ONT67_001790"
     CDS             18809..19549
                     /locus_tag="ONT67_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2355528.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ONT67_001791"
     CDS             19717..21066
                     /locus_tag="ONT67_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355529.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ONT67_001792"
     CDS             complement(21194..21784)
                     /locus_tag="ONT67_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCR2355530.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6293 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa
//
LOCUS       DAJSBM010000042        21872 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJSBM010000042 DAJSBM010000000
VERSION     DAJSBM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.042"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            8..1129
                     /locus_tag="ONT67_001793"
     CDS             8..1129
                     /locus_tag="ONT67_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCR2355531.1"
                     /translation="MVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPS
                     AGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGI
                     LIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAA
                     GISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALF
                     LGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLH
                     HRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIG
                     LINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1122..1922
                     /locus_tag="ONT67_001794"
     CDS             1122..1922
                     /locus_tag="ONT67_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2355532.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1922..2749
                     /locus_tag="ONT67_001795"
     CDS             1922..2749
                     /locus_tag="ONT67_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2355533.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2727..3461
                     /locus_tag="ONT67_001796"
     CDS             2727..3461
                     /locus_tag="ONT67_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2355534.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3561..4427
                     /gene="rfbA"
                     /locus_tag="ONT67_001797"
     CDS             3561..4427
                     /gene="rfbA"
                     /locus_tag="ONT67_001797"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR2355535.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4441..5013
                     /gene="rfbC"
                     /locus_tag="ONT67_001798"
     CDS             4441..5013
                     /gene="rfbC"
                     /locus_tag="ONT67_001798"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR2355536.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5035..6063
                     /gene="rfbB"
                     /locus_tag="ONT67_001799"
     CDS             5035..6063
                     /gene="rfbB"
                     /locus_tag="ONT67_001799"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR2355537.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6161..7012
                     /gene="rfbD"
                     /locus_tag="ONT67_001800"
     CDS             6161..7012
                     /gene="rfbD"
                     /locus_tag="ONT67_001800"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR2355538.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7047..9080
                     /locus_tag="ONT67_001801"
     CDS             7047..9080
                     /locus_tag="ONT67_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355539.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9124..10404
                     /locus_tag="ONT67_001802"
     CDS             9124..10404
                     /locus_tag="ONT67_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCR2355540.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10614..11420
                     /locus_tag="ONT67_001803"
     CDS             10614..11420
                     /locus_tag="ONT67_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2355541.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11432..12652
                     /locus_tag="ONT67_001804"
     CDS             11432..12652
                     /locus_tag="ONT67_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355542.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12642..14228
                     /locus_tag="ONT67_001805"
     CDS             12642..14228
                     /locus_tag="ONT67_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2355543.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14267..16405
                     /locus_tag="ONT67_001806"
     CDS             14267..16405
                     /locus_tag="ONT67_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2355544.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16773..17756)
                     /locus_tag="ONT67_001807"
     CDS             complement(16773..17756)
                     /locus_tag="ONT67_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR2355545.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18134..19525
                     /locus_tag="ONT67_001808"
     CDS             18134..19525
                     /locus_tag="ONT67_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2355546.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19541..20581
                     /locus_tag="ONT67_001809"
     CDS             19541..20581
                     /locus_tag="ONT67_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2355547.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20600..21685
                     /locus_tag="ONT67_001810"
     CDS             20600..21685
                     /locus_tag="ONT67_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HCR2355548.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            21718..>21872
                     /locus_tag="ONT67_001811"
     CDS             21718..>21872
                     /locus_tag="ONT67_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2355549.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNV"
BASE COUNT         7079 a         3566 c         4481 g         6746 t
ORIGIN      
        1 ttttagtatg gttataagat tttttctgac aattttatta tcactgatat taacacctgt
       61 attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa
      121 taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac
      181 tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc
      241 tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa
      301 aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga
      361 ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac
      421 gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct
      481 ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat attttttcct
      541 ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg
      601 tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt
      661 cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc
      721 atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat
      781 taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg
      841 actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt
      901 gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcgtatt
      961 gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga
     1021 gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca
     1081 agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc
     1141 agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata
     1201 attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt
     1261 cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct
     1321 attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg
     1381 attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag
     1441 cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta
     1501 atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt
     1561 tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg
     1621 aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta
     1681 tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt
     1741 actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca
     1801 caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt
     1861 ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct
     1921 gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc
     1981 agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga
     2041 taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca
     2101 tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga
     2161 aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga
     2221 actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc
     2281 tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc
     2341 agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg
     2401 gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt
     2461 ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt
     2521 acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg
     2581 ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag
     2641 tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca
     2701 tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt
     2761 cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt
     2821 tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata
     2881 catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc
     2941 ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa
     3001 tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact
     3061 attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg
     3121 agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta
     3181 ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac
     3241 ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg
     3301 gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa
     3361 acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc
     3421 tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct
     3481 taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc
     3541 aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc
     3601 tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga
     3661 tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa
     3721 cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc
     3781 agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag
     3841 aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg
     3901 gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg
     3961 gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag
     4021 ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt
     4081 atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag
     4141 aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac
     4201 ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat
     4261 ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa
     4321 ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga
     4381 aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag
     4441 atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc
     4501 gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg
     4561 aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg
     4621 cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca
     4681 gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga
     4741 tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga
     4801 ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca
     4861 gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat
     4921 ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa
     4981 gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa
     5041 aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta
     5101 aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa
     5161 gagaacttgg ccggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat
     5221 gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa
     5281 tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt
     5341 acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact
     5401 gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa
     5461 ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa
     5521 gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt
     5581 tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa
     5641 gtaacgagta tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt
     5701 gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga
     5761 attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa
     5821 ttgatcttag aaatgatggg ccaaccaaaa gacgcatacg accatgtaaa cgaccgcgca
     5881 gggcacgatt tgcgttatgc aatcgattcg acaaaattgc gagaagagct aggttggcag
     5941 cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat
     6001 gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa
     6061 taatacagag gaaatttcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct
     6121 gtctcttttt taaaataaaa atcaaggagc ggataaccat atgtttttat taactggagg
     6181 aaatggacaa ttaggtacag aattgcgcca tttattagat gaaaaaaatc tgccatatgt
     6241 ttcaacagat gcaaaagact tggatatcac agatgctgag aaaacaatgg cttatattac
     6301 agaattaaaa cccgatgtta ttttccattg cgcagcttac acagcagtag ataaagcaga
     6361 agaagaagca aaagaattag atgagaaaat caatgtcgat ggcacaagaa atgtagcact
     6421 tgcagcaaaa gctgtgaatg cacaattcgt ctatatcagt acagactatg ttttcgatgg
     6481 aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg
     6541 tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat
     6601 acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct
     6661 agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac
     6721 acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta
     6781 tcatttgtcg aacgaaaata gctgttcttg gtatgagttc gcaaaagaaa tattgaaaga
     6841 tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc
     6901 gcagtattca gtcatgagcc tgaaaaagac agaagcgtta ggttttgtca taccaacttg
     6961 gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt
     7021 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
     7081 aaaagccaaa agcgaatcag gcagattcga atttggtgct tgacctgttt ctaaaactcc
     7141 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
     7201 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
     7261 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
     7321 tctcacaagc tgaagaggac ttttcgttgg tgaacgagga ttattcatta aacggacaaa
     7381 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
     7441 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
     7501 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag aggaaaaaag
     7561 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
     7621 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgattgct ttttatcatt
     7681 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
     7741 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
     7801 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
     7861 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
     7921 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
     7981 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
     8041 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
     8101 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
     8161 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
     8221 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
     8281 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
     8341 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
     8401 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
     8461 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
     8521 tttacctcgc ttttgtcgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
     8581 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
     8641 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
     8701 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
     8761 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
     8821 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
     8881 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
     8941 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
     9001 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
     9061 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatatt agtagaaagg
     9121 agagtgaaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
     9181 ttgcttttgt tgctgacgct agtctttttt gtttggacag caggggtagg ttttccagca
     9241 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
     9301 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
     9361 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
     9421 ctgtttcctt tggttatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
     9481 gatcgcggta tttatctgat tgtctttacg gtcttcctcg gaatcacgtt atatcgtgta
     9541 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
     9601 gatatttgga tcaatacgaa tacaatcgga agttctttga tgactcttgc tttattgatc
     9661 agtggatttg ccagttcttt tgaacgatgg tatgttcgaa tcatcggtgg gatagcagtc
     9721 gttgcagcct ttttaggtat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
     9781 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
     9841 attgcctaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
     9901 gaaaaagtca atttgtttac cgatcgagaa gatatttggc ataaattcta tcagacgcta
     9961 ggagaaaaat ctgaacaagt tttactagga atgaaaacat ttatcttcca gcgaggaaac
    10021 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    10081 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    10141 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    10201 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    10261 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    10321 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    10381 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    10441 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    10501 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    10561 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    10621 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    10681 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    10741 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    10801 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    10861 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    10921 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    10981 acacccattt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    11041 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    11101 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    11161 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    11221 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    11281 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    11341 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    11401 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    11461 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    11521 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    11581 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    11641 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    11701 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    11761 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    11821 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    11881 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    11941 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    12001 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    12061 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    12121 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    12181 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    12241 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    12301 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    12361 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    12421 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    12481 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    12541 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    12601 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    12661 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    12721 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    12781 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    12841 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    12901 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    12961 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    13021 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    13081 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    13141 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    13201 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    13261 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    13321 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    13381 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    13441 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    13501 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    13561 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    13621 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    13681 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    13741 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    13801 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    13861 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    13921 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    13981 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    14041 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    14101 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    14161 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    14221 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    14281 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    14341 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    14401 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    14461 tgttaagacc caaagcggtt tcaaggtacg tttatcaggg aaaatgagag gaaaagccat
    14521 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    14581 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    14641 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    14701 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    14761 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    14821 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    14881 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    14941 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    15001 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    15061 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    15121 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    15181 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    15241 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    15301 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    15361 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    15421 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    15481 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    15541 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    15601 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    15661 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    15721 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    15781 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    15841 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    15901 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    15961 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    16021 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    16081 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    16141 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    16201 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    16261 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    16321 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag acggaatcgg
    16381 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    16441 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    16501 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    16561 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    16621 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    16681 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    16741 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    16801 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    16861 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    16921 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    16981 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    17041 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    17101 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    17161 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    17221 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    17281 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    17341 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    17401 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    17461 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    17521 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    17581 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    17641 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    17701 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    17761 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    17821 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    17881 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    17941 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    18001 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    18061 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    18121 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    18181 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    18241 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    18301 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    18361 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    18421 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    18481 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    18541 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    18601 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    18661 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    18721 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    18781 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    18841 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    18901 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    18961 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    19021 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    19081 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    19141 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    19201 gtcgatcgta ggaccacgtc ctgaacgttc tttcttcgtc gatcagttca atgcagaaaa
    19261 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    19321 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    19381 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    19441 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    19501 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    19561 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    19621 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    19681 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    19741 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    19801 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    19861 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    19921 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    19981 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    20041 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    20101 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    20161 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    20221 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    20281 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    20341 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    20401 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    20461 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    20521 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    20581 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    20641 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    20701 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    20761 attaggaggc cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat
    20821 agaagtgaat aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt
    20881 tggcggggga attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca
    20941 tagccatgca actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt
    21001 tttaaccaaa ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg
    21061 tgaaagtctg tttcaaaacc atccctttga gatagttcat aatggtattg atttagagct
    21121 ttatagtaaa aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag
    21181 tacttttgtt gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt
    21241 agagattttt ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga
    21301 agggtcactt agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt
    21361 aaaattttta ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt
    21421 aatgccttct ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt
    21481 aaaattagtc ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt
    21541 tgtatcatta gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc
    21601 taataaaata acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga
    21661 gagtatatat agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg
    21721 aaaatatgtt atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat
    21781 cgattgaacg aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca
    21841 tttattgaag catttaaatc atatgataat gt
//
LOCUS       DAJSBM010000043        21527 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJSBM010000043 DAJSBM010000000
VERSION     DAJSBM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.043"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            41..301
                     /locus_tag="ONT67_001812"
     CDS             41..301
                     /locus_tag="ONT67_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355550.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            634..1206
                     /locus_tag="ONT67_001813"
     CDS             634..1206
                     /locus_tag="ONT67_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355551.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1207..1518
                     /locus_tag="ONT67_001814"
     CDS             1207..1518
                     /locus_tag="ONT67_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR2355552.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            1509..1769
                     /locus_tag="ONT67_001815"
     CDS             1509..1769
                     /locus_tag="ONT67_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355553.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            1930..2289
                     /locus_tag="ONT67_001816"
     CDS             1930..2289
                     /locus_tag="ONT67_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355554.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2286..3929
                     /locus_tag="ONT67_001817"
     CDS             2286..3929
                     /locus_tag="ONT67_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR2355555.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            3964..5046
                     /locus_tag="ONT67_001818"
     CDS             3964..5046
                     /locus_tag="ONT67_001818"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355556.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5090..6262
                     /locus_tag="ONT67_001819"
     CDS             5090..6262
                     /locus_tag="ONT67_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR2355557.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6249..6953
                     /locus_tag="ONT67_001820"
     CDS             6249..6953
                     /locus_tag="ONT67_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR2355558.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            6958..8118
                     /locus_tag="ONT67_001821"
     CDS             6958..8118
                     /locus_tag="ONT67_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR2355559.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8194..8472
                     /locus_tag="ONT67_001822"
     CDS             8194..8472
                     /locus_tag="ONT67_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR2355560.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8453..8824
                     /locus_tag="ONT67_001823"
     CDS             8453..8824
                     /locus_tag="ONT67_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCR2355561.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            8840..9244
                     /locus_tag="ONT67_001824"
     CDS             8840..9244
                     /locus_tag="ONT67_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355562.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9241..9651
                     /locus_tag="ONT67_001825"
     CDS             9241..9651
                     /locus_tag="ONT67_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355563.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            9712..10299
                     /locus_tag="ONT67_001826"
     CDS             9712..10299
                     /locus_tag="ONT67_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR2355564.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10372..10839
                     /locus_tag="ONT67_001827"
     CDS             10372..10839
                     /locus_tag="ONT67_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR2355565.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            10885..11193
                     /locus_tag="ONT67_001828"
     CDS             10885..11193
                     /locus_tag="ONT67_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355566.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11388..18122
                     /locus_tag="ONT67_001829"
     CDS             11388..18122
                     /locus_tag="ONT67_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR2355567.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18126..18998
                     /locus_tag="ONT67_001830"
     CDS             18126..18998
                     /locus_tag="ONT67_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HCR2355568.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            18995..20044
                     /locus_tag="ONT67_001831"
     CDS             18995..20044
                     /locus_tag="ONT67_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355569.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20048..20872
                     /locus_tag="ONT67_001832"
     CDS             20048..20872
                     /locus_tag="ONT67_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355570.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            20890..>21527
                     /locus_tag="ONT67_001833"
     CDS             20890..>21527
                     /locus_tag="ONT67_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HCR2355571.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7737 a         3219 c         4559 g         6012 t
ORIGIN      
        1 aatacatcaa cagctggtta tgcgcttgcg gaggtacaaa atgaatcatg agaagtttat
       61 cgaaaaatgc aaggctattg tacgtgaaag aattgaaaat gagattgctg acctaagtgg
      121 agcagtgcct gagtttagta ttttcatagt ctggtcatgc aaaacactgc aaaatagcaa
      181 agcattagtc agcgctagct taaaaggagc accgtatttt gaaattacgt tgaacgggga
      241 caaaggtgaa atctatgtag atacttatct caaaaaatca aatgaatgta tcaaagtcta
      301 gcaggtgcta ggctttttct ttacataaag gaggctgcat aatgagaaac tactggtatg
      361 tatcactaac taatgaatat cctcgaacca ttggtgattg ttcagtgcgt gttgtgcgtt
      421 ctgtacaaat caaagggaag tactctattg tcgaaatgct aagagaagct acaccaaacg
      481 aagtggataa atgcaagctg atatattgcg gtcatggcta ttggaaagac gagtatatcc
      541 aatacaacat tgagaggtac atcaaatgaa tgataactat gattacatca agttgttagc
      601 aagtaattat tagatgtttt taggaggaaa actatgaaat taaaagatta tatcagagaa
      661 gggtataacg ttgtaactgc acccaatcta gcttacaaaa ttcaagaaga ttatcccaat
      721 gctttagtgt ttactgatag agctttagat gccattccta tagggaagtt attagtagat
      781 cgtttttata gcaataatcc agctgttctt agtgctaagc cacttcaaaa cgcttatgct
      841 attgaacgct ttacttgcga gtttactgga tatgagacgg tagtaccaat gacaggtgag
      901 acaggaagaa aagtaattca acagatcaaa aagaggattg ctgaattgac gttagatgat
      961 ggaagtaata cgaaccttct agaaatcaaa ctaagagata ctgactcagt accagaagtt
     1021 tattataaag gtcaacgaat ggatgagatg ccgataggtc ttgtcgatat tacttatcat
     1081 tgggatacgg aagaatatga atgccaagta ccaaaaggag ctaacgacat cactattgaa
     1141 tattatgatt cacataataa caagtactta gatagaaaaa tcattgggca caagagagat
     1201 atgtaaatga ttgatgtttc aagtaaacaa gcaagagcta ggttctatgg atcaactgag
     1261 tggaggaaac ttagaagtca atgcttagag cgagatcatt atgagtgcca atggtgtaaa
     1321 gcagagggta gactaacaac tcaacatgat tcaattcttg aagtggatca tataaaggag
     1381 ctggagtatc atccggattt agctctagat ataaacaacc taagaacctt atgcaaagat
     1441 tgccataaca aaaggcacaa ccgtatgaaa tatcgtggcc aacaaaagaa aagaaagtgg
     1501 gatgatgaat ggtggtaaga aaaacagcaa acagagatga tgtcaaacga gcagaagcaa
     1561 tcgctatggt tgataaggct attgataagt tggttctatt taagagtcag ctcattgaag
     1621 gaacaacaac tgtagaggat atatctatca acaatagact aataggtaac gctaatgaag
     1681 taacagggac atttaatatt aatgtagaca ttgattatac cttgagtcca tttgcacata
     1741 ttgtagaaag caacgagcaa gacggctgat aaatcagtat aaagcgaaga tatatagagt
     1801 gaaatacccc cggtcaaaag attttgagaa gaaataaaat ttttgggaac cggtggatgg
     1861 ggtcaactct gcaaataaat gatgctattt tcacacgacc ccctccccac gtagaaagga
     1921 agtgagttga tggctgatct gcaagagaga aataggcttg tttctgctga agaaaggcgt
     1981 ctaaataaat tgtttaagga tattgataag gataaaaaga aggtcgtttc tggtttgatt
     2041 acgcaagcgg cacgcttaaa aattcttctt gatgaaatgt ggattgatat ttccgaaaaa
     2101 ggtgattatg agcttttctc tcagtcagaa aatcaaaaac cttacgagcg ggaacgacca
     2161 gttgctaaac agtataattc gagagaccaa gcatatcaac gtgtaattaa gcaacttact
     2221 gactatttgc cagaagaaaa gcgcgaacct acaaaaaatg cagcgctaga tggtagtgat
     2281 ttgctgtgac aacattaaag ccattctatt ttgatgaata cgtagattta tacgaaaaag
     2341 gggtaatccc ttttaataaa gaaagaattc agttaattac ttatttaaaa aacgaagtgc
     2401 tagttcggga tgatatctat tttgatgaag aaatgattca aaagtttatc aagtacactg
     2461 aaaaaaattt ttttccttta gctaaatatc aaaaatttat cacacctttc attttctgct
     2521 atcaaaaaga ggacgacgag gtgttttttg atgaaattct aaatagtatt gcgcgaggcg
     2581 gcgggaaaaa tgggttcatg tcagcaagag actcattttt tattagccct ctctacggtg
     2641 tcagaaatta tgatgtaacg attacagcga attctgagaa gcagggaaag gtatctttta
     2701 aagaagttta tgaaatggta caaatgcgca aactggaaaa acaattttat ttgactaaga
     2761 tggctatagt caacaatgta actaattcga tttttagtta taggacaaac aatcctaaga
     2821 ctatggatag tgcaagagat ggctgcttag aatttgatga aatccatcag tttgaatctt
     2881 cagacctagt tgatattcag cgtagtgggc tagggaaaat aaaacatcct cgaacttttt
     2941 ataatggtac aaatggtcat gttagagaag gtttttatga caaaatgttg gaacgagcac
     3001 aaaagatttt tagtggagag aacaagaatg atcgcttgtt tccctttatt tgtaaactgg
     3061 ataacattga tgaaatggat aatcctaaaa tgtggtcaaa ggctaatcca atgttcgaag
     3121 aagattcagc atattcaaaa cgtctgtttt ctacagtgat gaaagaatat aaaaaactag
     3181 aagatgagcc atcaggtagg cgtgaatttg ttgttaaacg aatgaatttt acagaaggtg
     3241 atgcagaatc agatattacg actcatgaaa aattaatggc tactaaacaa gaattgccaa
     3301 atttgaatgg tcgttcttgt gtggctggat ttgattatgc aagtattcgt gattttgcaa
     3361 cagtgggttt gctttttaaa attgatgata aatttatctg gcagcaacat agttttgcta
     3421 ggaaaaagtt tctagataca ttcaaactga aagcgcctat taaggattgg gagaaaaaag
     3481 gtttagtgac aatcgttgat gagccatcaa ttgatcctcg tcatttaatt gattggttaa
     3541 atgaaaaaag aaaaatccat caaatagaaa tcgtttgtgc cgacggattt cgtatggatt
     3601 tgctgaaacc tcttctagag aaagaatcat ataattttga gtttatcaga aatataagag
     3661 gtgtgcagtc aaaagttgct cctattattg aagatggttt tgcaaatgaa cggtttattt
     3721 ttggtgatga tcctatgatg cgttggtata caaataattc gtttatcaaa gaagacaaat
     3781 tagggaataa aacatttttg aaaaaagagc cagtaaggcg taaaacagat gggtttcatg
     3841 cttttttggc agctttatat aaacgagaag aaataactga tatggattat gaggaagcat
     3901 ttgatatgtt ggatgagatt gaattttaag gtatactcct tattgaaagg ggtagttata
     3961 gaagtgaaaa agagatattt tctatttgtt acaatattta taataatagc aatttgtgtt
     4021 ttgacagtat ttttaccaca aattactgag tatttaaatt atcggatatt taccacaaaa
     4081 gtagatgata gcgttactag tttaagtagg ctagaattta taaaaatatg tgcatctgtg
     4141 tttggtccta ttttgactgt aatggtattt gtgaatactt taaatatgca gaaaaaaaca
     4201 gagcaaaagc aaaatgagct tgaagtaaag caagctaagg ctgagaacga gagagcaaaa
     4261 aaagagctgt taacacagat agatcgtgag ttctattttt tattaaatat gtttattgaa
     4321 atacaaaaag atcaaaatgc caaacaagct gttattgctt tacagaagtt ggcaattttt
     4381 aacgaaaagg gttttaatca agactatgca tttacttcta aagatttatc agatagtgaa
     4441 attgggagta caattgaaca atcaggaccg ctgaagggca cagttgaata tataaaattt
     4501 gaaacatttg atgtaagcaa gtcgatgggg aattatcaaa caaaaacaaa agacgttgct
     4561 acaagtttac acgatcagaa agtagctata ttgaatgaac aatatgaaaa gatgcaaata
     4621 tatcttggac ggtacttcaa aatgtttcac cgaatagtta aaacattaaa tgagtattat
     4681 gacgattata atgattttga tgtgaagagg tatacaaaat atatagggac attaagaaca
     4741 caaatttctc cagctgaatt ccaagtgatt ttattcaatt ctctatatat aaaacgaggt
     4801 tttggcttag gaattcaact tattggaagt ggtttttttg gagatgattt tgattttgaa
     4861 actaatcaac attttgaaac aagtatcaat gaacagtggt ttctatcact atcaactgtt
     4921 gacagtgata actctataaa gcgacaaaaa ttatctgaat atattaaaga attaggatcc
     4981 gtagaatata aaaaaatagc taattttgaa agtttatata aactctttaa tgaaactaag
     5041 ctctagaaat agggcttttt tatttttctt tgaaaggtgg tgaaaacatg tgagcttatt
     5101 tgatatcttt aagcagtcaa taagaaatga agagccttct gagtggatac cggattttgt
     5161 atatggagag gaagtatctg ctcgggcata cctcaaaatt atggcaaaaa atactgtttt
     5221 agattttgtt gcaagaacta tgtcaacgtt agaagttaag tttaaaaata aagatgggac
     5281 atcagactgg gattatattc taaatgttcg acctaattct gatatgtcag cagctacatt
     5341 ttggcaaaaa tttttttatc ggctcttaga tgacaacgaa gtgttagtaa tatttacaga
     5401 tgataatcaa ttgctgattg ctgatgattt tagtcgaacg gagtatgccg tgtatgatga
     5461 tgtttttaca aatgtgatgg taaaaaattt tgtctttgaa cgttcatgga acatgtctga
     5521 cgtcatttat atggaatata ataacgaaga attagaaagg tttacacgag gtttatttga
     5581 agattacgcc gcgttgttcg gacggattct agaagttgca atgcgtaaca accaaattcg
     5641 tgggtctgta tccattgatt caacgggaac ggcgaatgat gaaaaaggaa aagatggtaa
     5701 gactagatct caaagattac aagaatatat tgataggatt tggaactctt ttaaaactaa
     5761 ctcagttgca attgttccca aagtgaaagg ctttgagtat gaggaataca ccaataaaca
     5821 aggcgtatcg aatcaatcgc tagatgaact gaataagatg aagtcgtctt taattgatga
     5881 tgttgccaac gccataggag tacctacggc gcttatttat ggtgaaaaag ctgaactgga
     5941 ttcaaatatt aaagcttttc gtaaattgtg tattgctcca ctagttaaaa aacttcaaga
     6001 cgaattggtg gcaaaaataa ttagtcgtca agagtacaaa aacggggaac gtatagaagt
     6061 taaaaaagtt ctccaaaaag atctattaga gtatgctact caaattgata aaataacatc
     6121 tggtggtgcg ttttatattg acgaagttcg tgatgaagtt gattatgatg agttgccaaa
     6181 taatgaagga aaaattcgta ttcgtacgaa aaactatgaa gaaactgtga agggaggtga
     6241 gaacgacaat gccgaaagct aaaaaagtac catttcagtt tactaacgaa attcagaatg
     6301 gtaagcacat tctcaccttg agtggaaatg tacagaaaaa atattggcgt gatgatgatg
     6361 tcataaacgc taaagatatt cgtgactctc tagatactgt aaccgatgat attgtaatca
     6421 agttgaacag cccaggcggt gatgtgtttg aagggattga gatttacaat tatttaaaag
     6481 atcacccatc aaatattact gtagaagtta cagggcttgc cgcaagcgca gcaacgttta
     6541 ttgtcgctgg tgccaatgat gtgattatga atgttgggac ttctttgatg attcatgaag
     6601 cttctacatt tgcttggggg aataaacagg acatccaaaa aacgttaaac gcattggaaa
     6661 cgatcgatga ttctatctta gctatttatt cagataagac tggccaatct gttgataagt
     6721 tgcgtgaatg gatgaatgaa gaaaaatggt ttacagcaga tgaagctgtc aaatatggat
     6781 ttgccaattc tgtgaagcgt gaagaaccac aaaatgaacc tcaaaatatt gcagcgatga
     6841 ttcaagatgc tgttgccgtt gcaatagcga atttaaagga acctgttact aataaaatcg
     6901 aacaagaatc aaaacaaaaa tcattaatcg cacgattgcg aaaaggagaa taaagatatg
     6961 ttaaaaatta cagataaaac tgcagatgca aaaaaggcat ttaatgctat ttcttcgaaa
     7021 gaagatgcaa cacccgaaca aattaatgct gctctagaag aatatgtaac cgccattgca
     7081 gaagatgccg gcaaacaggt gcgtgctgaa tatgaagaat tgaaaaatgt gacggacaac
     7141 cgtgtattag aggctcgtgg aattcctaca ctaacagcag aagaaacaaa gttttataat
     7201 gaagccgtaa aatctggagg tttcgattct gacgtagttt ggccagaaac gattttggaa
     7261 cgtgtttttg aaaatcttca aaacgatcat cctattttag gcctcattaa ctttacacca
     7321 actgtaggtc gtgtcaaagt aattagagca cgtcgtaaag gtgttgctgt ttttggacca
     7381 ttgcataaag accttgaagg ccaactagat gctgaatttg gtgcaactga atttgttcaa
     7441 cttgcgttga ccgcattttt cttaatttct aatgatacct tagatttagg accacgatgg
     7501 attgaccgtt ttatcaatct ttctttaagt gaagcagtac gtgatgtttg ggctaaaaaa
     7561 attattacag gttctggtaa agatgagcca attggattgc ttaaagatct agatggtgcg
     7621 gtcacaagtg gtgtatatcc agataaagct agcgcaggga cattaacttt tgcgaaagag
     7681 aagattgttc ttgaacttgc aggggtgatg aaaaaattat ctaaatacac atacaaagta
     7741 gatggtgacg atgaaggtga agtgaaatac cgttctgttg ctggacgagt gtatctaatt
     7801 gttaatcctg ttgattactg ggatatcgta gctagagtta catttgcaaa tttaaacaat
     7861 gtgtatggga ctacgatgcc atttatcgct gttgatcata tcatcgaatc tgtagacgta
     7921 ccagaaagta aatgtattgc atacgttgat aaagagtatg aagctactca atcaagagct
     7981 gaaaaagtct atgtatataa agaaactttt gcaatgaaac gcgcaacttt gtatgctgtc
     8041 gacatgcttg gtaatggcta tccaacaaat aatgatgcag cacaagtata tgacttggac
     8101 tttacagcgg gggagtaatg tcccctgcaa ttggtgagat aacaccgacg actgatgggg
     8161 cagtcattaa tctttcgtaa atggggttag agtatgaaca ctgaagaatt tataaaagaa
     8221 tataaggccc gatttcgtat tttccatact tccgaagatg aggatatcgg gaaacagtta
     8281 gagagcgggt ttgccgatat aaaatctcta atcggcagtt ttgatccttc cacttatgag
     8341 aaaggaaaag aattagttta tgaacgtact cggtatctac ggaacgaatc actagagtac
     8401 ttttacgaca attttcaaac aatgattatg gatgcatcaa ttgatctggc aggtgctgga
     8461 tatgccaatt aaatcaaatt ataagaagcc taaaattgta gctggtgatc taaatacgcc
     8521 agttactttt tttgagtttc aacctaataa gggtccagaa cctggtgatg aagaaaaaaa
     8581 ggaactgtat ttttgtacag cactagttta caacccatca atgaaagatc gtgacattct
     8641 tggcacaaaa gaaggcgtta cgataaaaat acgggatccc catcaatctt atattcctac
     8701 taacaaacat aaagtaagga tattagatta tcgatatgga gataaagttt gggaaataat
     8761 cgatgtctca ccggatttgg aagaaaattc aattgtaaag atagtattag aattaattac
     8821 ttaaatttgg tgataaatga tgagtaacgt aaagtttgaa ggcgttcaag aaacaatcca
     8881 agctttagaa aaaaagtatg gagaaaagaa aactaagaca ttaacaaaaa aagcaatcaa
     8941 tgttggcgct gaaaaagtag aaaaacagtt gcaaacggac atgaccgttt ttaaagacaa
     9001 aggttatacg atcgctgagg ttgttcgcaa gaacgctact tatagagatt acaaggcaga
     9061 agctgaaatc ggttggaatg gtccacatca acgatataga atcattcatt tgaatgaatg
     9121 gggatataca cgaaagggca aacaaattag accgagagga tttggtgtaa taacaaaatc
     9181 tcttaagaat tccgagctgt tgtactttga tgctgtagga gaggaggtta aaaagagttt
     9241 atgaaagata tgcttaatat catttacgag gctctttgtt tgaatgagta tattcattca
     9301 atgacctaca acgagaatac cgagcaatat cgtattaaat tttatgagca acctgaaacc
     9361 gccgataaaa caggtccaat gatcacaatt cgtcctgtag atacacctaa cgaagcgtac
     9421 cacggaagta acaaagagct ttctgtggag catatgatac agattgacgt tgaatcgaaa
     9481 tatagagcaa catgcaagca gattcaatat gagataaaaa aagagatgaa aaaactaggc
     9541 tttggccaaa tttccggtca agggttagat gaatacttta ctgaaacaaa ccgctttgta
     9601 gacgcgcgtc gttacgaagg aaatacaaaa atttacgata ccgaatatta gaaattaaga
     9661 cacgaaaata acgtgtcttt ttttattacc caaaattagg aggaaaattt tatgacttta
     9721 gtaggattca aaaaaatgac aatcggggtc tttgacagca cagggaagat tccagcagca
     9781 aatttatatg taattgaagg tgaacaaaac aaaggggcga ctgtatctgc tgaaatcagt
     9841 gggctatcaa aagaaccatc aaaagtttac ggatcgaaca tcgcttacta tgtctctcaa
     9901 aaaggaacag gcgatgtttc agctactttt ggattgttag atttaccaac agaagtaaat
     9961 gacaaaattc ttggttacaa aaccgataca aacaaaatca gcttcttggg agaagatact
    10021 gagccaccat attgtgcaat tttgatggaa tcggaggatt tgaacggcga tacagcaatg
    10081 cttgcgatgt tcaaaggaaa attcagtcgt gaatctatta atcttaacac tacgacaaat
    10141 gaagctttcg aaccagaagc agaagaatat gtattttcag cgattgccaa tgatgttgaa
    10201 ggtgatgcta aaggccaaac agtcgcaaaa tatgttggag acgaagaagc ttctattact
    10261 ggtttgaaag agatggtatt tcctgcagcg ggggagtaac tagccccgtt gttggagcaa
    10321 tcactcccac tattaatggg gcaacagttg aattaagtta ggaggataac tatgccagat
    10381 acatttaaaa tctataaaaa aaatggtact gaatttacaa aagtcgcaga gggtgaaagt
    10441 ccactttcta ttactggaat tgcagcaaat actcaagttg ctaaaggaga ttatcaagct
    10501 acgcgtgtgg tcggaggtca agaatcagta aaagtagata ttcctgcatt caccactctt
    10561 ccaatttcag ttactggtgt gacgttggat aaaacgacag ctgaagttga acaaggcgga
    10621 accttaaaat taactcctac tgtaacacca gcaaatgcaa cagacaaaac aggaagctgg
    10681 agcagttcga atacagctgt agctacggtt actggaggca acgttacagt aaaaacagac
    10741 gctgaagttg gcggaactac agaagtttca tttactacta cggatggcgg taaagtagca
    10801 aaatgtacta ttacagttat tgaaaaagca gcgggctagt cttcggacta gcttttttat
    10861 ttggaaaata atggaggaat ctaaatggca aaagtaagaa ttgaattgaa agaagaatca
    10921 ggaaagaaag tctatgaaaa catagacaca acagggaaag attatcgaaa agctttagaa
    10981 acaatcaaaa agctaaatga agacggagta atgatttgga atcgtttaga catttatttg
    11041 gattttgctg taggtatctt cagggatagc aaattgacag ctgaacaaat tcttgatggc
    11101 ttaccatctg agaaggtaat gagcacattg gacgacatcc taggagaagt aatggggatt
    11161 gaaagtaatc cagacccaga cgcaaaaaag taacacccga agaagcagaa gaaatgtact
    11221 taaacctatg cagagagtta gtgaaagcgg gatggtcact atctgacata gaagataatt
    11281 cctttgaaat gatgatgaaa attgcgtgca cttcgcctaa gaaagagaaa caaaaagaag
    11341 ttgatcttaa agacttcttg aaatccatgt agagaggagg taaacatatg gcaaatggaa
    11401 aacctttagg caatatgatt atcaaactag acttagacag ttcagctttt tcaaaaggtc
    11461 tagccggagc taaaaacgct gttaaccatc aaatgaaagc aatgaaatca cagatgcaag
    11521 taatgaactc ttcaggaaat tctctcggtg ctttacaagc aaaatacaat ggtttaggcg
    11581 gagttctaca agcaaatgaa aagcaagtag aattgctcac taaggcttat aaggatagtt
    11641 ttgactctaa cggaaacgct actgcttcga ctgctaaata cgctaatcaa ttgaatcaag
    11701 caacagctag atcagcaagt tacgaatctc agatgaaaac aactgttgga cagattgctc
    11761 ggatgaaagt ggaaacggaa gggtttacgg ggaaactaaa ggctaattct gatgtattga
    11821 ttaattctgg gaaaaaaatt gaagcctttg gtaaaggagt ttctgggata ggatctgccc
    11881 taactgttgg agtaacagct ccaatagtgg caggcgcaac tgctgttact agtgcagcta
    11941 tttcatggga aagtgctttt gcaggtgtaa aaaagacaaa tgatgaagtt gtagactcga
    12001 atggaaacgt agtttattcc tatagcgact tagagaaagg tctgcgtaat cttgccaccc
    12061 aattacctgg tagccatcaa gaaatagcaa atgtagccga agcagccggg caattaggta
    12121 taaagactga aaacgtagtt agcttcacta aaacgatgat agacatgggt gaatcgacta
    12181 atatgtcggc tgaaactgcg gcaacttctt tagcacgatt tgctaatatt acccaaatgt
    12241 ctcaaaaaga ctttgataaa ttaggatcag tcattgttga tctaggtaac aattttgcta
    12301 caaccgaatc agaaatcact gaaatgggat tgcggttagc gggagctggt aaacagattg
    12361 gaatgagcca aggcgaaatt ctaggatttg ctactgctct atcttctgtt ggtgtcgaag
    12421 ctgaagcggg tgggtctgcc ttttcgaagg tcatgatcca gatgcaacta gctgtagaaa
    12481 aaggatttgg cgcttttgat cagctgaaac agatggcaga agaacaaggt gttccgtggg
    12541 ttaatttagt aaatgctgtg agagacggtg gaaaatcgtt aaaagcagta tcagagcaaa
    12601 tgggattcac ttcatctgat ttaaagaaaa tgtataaaga agcggacaat tcgaaaagtt
    12661 cgcttgaaaa ttttgcgaat gtggcaggtg ttacaagtga acaattccag aagttgttca
    12721 agagtgatcc ttcagaagca atcatcaagt tcattcaagg gctgaaagat tcagaaaaac
    12781 atggtacttc agcaatcaaa gttctggatg acatggatat aaaagaagtt cgactacgcg
    12841 acagtttatt gagagctgca aatgctagtg acatattcaa aggagcaatt gaaaaaggaa
    12901 acggcgcgtg gaaacaaaat acagctttaa caaatgaagc taataaaaga tacgaaacaa
    12961 ctgaatcaaa gctaaagata ttgaaaaacg aagtgattga tacagcaatc gatcttggag
    13021 gtccatttgt agacgcgttg cgtgatggtc taaatgtatc gaaaccattg ataaaaaact
    13081 tagggaatat ggcgaaagct ttctcagcta tgagtgatga tcaacaaaga aacattatca
    13141 aatgggttgg attagcagca gcagctggtc cagcgctgaa gcttttagga actggtactt
    13201 cagtaatagg taaagttact tcaactactg gaagattgac gaaaagcctt gttgatttag
    13261 cagcgaaagc agcagaaaag aaggctatgg atgcctttgc atcgtcggta acaacagctg
    13321 gtgctgcagc tgcaaagaca gcaggagctg gaggtattgg cgggttagtc cctatgatgg
    13381 gaaaaacagc tagtgctgca agtactgctg cgggcgccag cggaataggc gcaatgacaa
    13441 cgtcactagg tttgttaggt cctgctctac ttggtattgt tggcgttggt ggcgcactgg
    13501 cagttggcta tggagcatgg aaattatttg gtgaagaagc ttggaattcc tcgcaaagag
    13561 taaagcaatg gggaacagat gtcggtgagg agatagatag tactcttgac ggagtacaga
    13621 agaatattga aggtgcaaat ggtcaattcg gtcttctcaa agagggtttt actgttgatg
    13681 atgcaaataa tatggcggct aatttcgcaa ccgctggaac atctttggaa acatctttaa
    13741 cgggtagaat aaaggcatta gacgatgcac taaaagggct acctcaatca gttcaggatg
    13801 ccatgaaaga agtagctgaa tcagaagaag aaaccttagg gcaatctctt tctaaaataa
    13861 aagacaacaa tgaagaaata aaaaaaatca gagaaagagc cgcaaatcag aacagagact
    13921 tgactgtttc agaactacaa cagatacagg atctaatgaa aggaacttct caagcgtatg
    13981 tggatacgct cgatgtttct gctaaagaca gaaaaaagat actatcagcc atgaatggtg
    14041 atgttgcaca agctacagaa gaagaagcga agacgtggtt acaatcttta gcgaaacaaa
    14101 aagctgcaac taccgaaaat tatagacaaa tgcttgaaga aaaaaaggcg gctttaaagg
    14161 aaaagggagt aagcaaagaa ttcattgatg cattagaaga tgaaatgaat gattacatca
    14221 aaactacaaa tcaaggattc gatagccaaa tagctgctat aacaaataaa tatccacaat
    14281 tgattaacga agtgagttta tcaaatggtc aattatttag caaaacggca gctgctatgg
    14341 acaatactgg tcagtataca aaagtttttc tcgaaagtaa tgaaaagatt ttagcccaag
    14401 cgaagaaaac atcggatgaa atagcagata atgccgaaaa aaatgcagaa caacttggta
    14461 tggtagctga tgaggctacg catgctggat caacttggaa tggcttagtt ttagacgaaa
    14521 aaacaggaaa agtaaaaact aatgtacaag aagtagtgaa tgaagcaacg aaggatttca
    14581 ctaaatggaa tgaattgaaa cctgttgttc atgatgcgaa actaacaacg aatgcaaaag
    14641 atgtcatttc catagcggca gtacaaaatg gctattggga tgatatggaa tggaaagaaa
    14701 aagaagcatt attagaagat gagtgttcta aaaatgtata caaggcttta gaaaatgctg
    14761 gtaaatggaa tgagttagat attccttcta aagaagcaat cctaacttca aatactcctg
    14821 aagttatggg tgaaactctc atgaatttgg gattatggga tacttacaaa cttcaagtga
    14881 aagatttaga tttgaatgcg tataaagtat acgatgctat cgatggatca gaagaaaaat
    14941 taaatagttg ggctcaatta gataacccca caaaagaatt gttattagat aactcagatt
    15001 tttctacaaa actttttaat tccgaagaaa tgtttaaacg ttggaatcag ttatcatcta
    15061 atgcaaagtt tctttcggct aataatacta aatttacaga ggttctttta tcatctgaca
    15121 gaatgtggaa tcaatgggta aaccttcctg ataatgagaa aagattactt gcagataacg
    15181 aagatttagc taccactgtt ttatcttcag aagaaatata cggaagatgg actcaattac
    15241 cagataatca aaaatatatg ttagctgaca atactgactt agcaaacaaa atattcgctt
    15301 cagaagaatc atggaatgca tggaaagcaa ttccagacac agttaaaaac ttgcttggta
    15361 ataatattga tttactggct aaagtgaaag acgggactgt aagtgtagaa gaatataaca
    15421 aagttatgcc acttcttaaa actctatatg gagataacgt taatttaaaa gaggcaatca
    15481 gtggggctaa aagctctata gagaattaca acaaaaatat ttttcctagt gataaaactg
    15541 cagtaggaca cgatgaggct tcacaagctg ctaaagatgc atttgaagcg ttcaacatct
    15601 tccaaacaaa aataccagat aaaattacta aaactgtatc agctgacttt ataggtccta
    15661 tgccgaaaaa tgcgaaagga acaaacttcc atcctggcgg agcagctatg gtaaacgatc
    15721 aaaaagggcc tacttatgaa gagcttataa ccttgccaag cggagaaact tttataccta
    15781 aaggacgtaa tgtcgtttta gacttaccaa gaggttcgaa agtactgaac gccactaaaa
    15841 ctaaacgtct agtgcctaaa tatgctgatg gtataggaaa cataacgacg gtttcatcta
    15901 cgctaaactt agatgcttta attttagcta ttaacgagct aacgacggta ttgagaactc
    15961 aactgatggc aggaacgact gatgctaaag atactaaact atcagatgaa acaaaagttg
    16021 ccgatccgat tattccggac agtctttctg aaaaatcaga tcagtatcta ggaattggag
    16081 ctcaatggtt aactaatcta atgaatggtt ggaactcagc ggttcctcaa tatatgaata
    16141 gtgaaatggt ttttattaca aactatctca atgcgctaaa acttcaaaac aatcctaatt
    16201 atacgcaagg agcaacatgg aataagaact tactgaatgg atggaatagt ttaactggga
    16261 cctttattgc tacaatcaat tcattttgta atcaagcaat ggtcacgctt agaaactaca
    16321 acactcctat gtacaacaat ggccgaacgt ggcagcaaaa taatcttaat ggttggaatt
    16381 cattgtatgg atcattcata gcccgtgtaa atcagcttgg taacgattcg attaacaacc
    16441 ttcgttctaa aagtggtggt ttttacaatg cagggacatt cttgttgcaa tctttaataa
    16501 acggaatgaa ttcgttaggt aattctcttt ctactacaat gaacaatgtc gctaatacaa
    16561 tggttggtgg tataggcaaa ggagttaatg gcgtcatatc gggagttaac tatgtgctta
    16621 aggaagtcga atcaagtaag aatatcggaa actggccaat tccacaatac gctaagggaa
    16681 ctgatggaca tcctggtgga cttgctatga ttaatgatca aaaaggccta gttcacgaag
    16741 aatatgttca gatgccagat ggacgtagtt ttattgccaa aggtagagat cttcttgtta
    16801 atttgcctaa aggtgctcag gtcttaaatg cttctttaac aaagaaatta aaggaacgtt
    16861 taaatattcc acactacgaa aaaggtattg gcaatttaga tattgttgat ttgttagatg
    16921 atgaaaaaag aatgttagaa ttcttaacta gcaaagttga tttttcaggc attaatgaac
    16981 catggcttga tatgactaaa tcaggaacgt cattaatgtc caaagctgca aatacaatgc
    17041 ttcaatcaaa attaagtgaa ttcttcactc atggcaattt tgatggtgca gtaaatgcca
    17101 atggtgttta tcaatattta gttgatgtgg cacagaaagt aatgggtaag ttccccggac
    17161 ttacagtaac ttctggatat cgagcaggag atgcctacta tcacggaaaa cggcaagcta
    17221 tcgacttggc ttatccaggt atttctggag atccgagata tacagcagca gcaaattacg
    17281 cttttgaaaa attcccttca aaaattgcgt atgtcattac gaatggacgt gtacgtgacc
    17341 gcatgggatt atctggaaca aggtcaagcg gacaatggac gaattggcca gatggcgatc
    17401 acttcgatca tattcattta aacggttcga tggggtctgg cgatatattc aatggaggag
    17461 ttggaggaag tggtgttgca cgctggcgtt cttatgtgtc taaagcattg aaaatgaatg
    17521 gattaccagc tacagcagcg tatataaatg cctggatgtc ccaaatccaa acggaatctg
    17581 gtggtaatga gaaagcaatc ggcggcaatg atgggctcgc cgaaggcaat gcaacaggat
    17641 tacttcaaac gaaacccgga acttttgcag ctaacgcttt tcctggtcac ggaaatatta
    17701 tgaacggatt tgataacatg ttagcagcaa tcaattatgc taagaaacgt tacggagttg
    17761 caggtatgtt gcaagttatc gggaaaggac atggatatgc caatggcggc ttgattacca
    17821 aagacggact atatcgagca ggagaaggaa ataaaccaga aatggttatt ccattgacaa
    17881 gaaaaacaag agctatcgaa cttatgggtc aagcattagc cttcctttct ggaagcaata
    17941 aaaaagaaac gaaaagtgag attaattctg tagataatag acatatccta caagcgatag
    18001 aaaaacagtc tcgtatgaca aatgatctgc tatcattatt actctcgttt tttaaaagta
    18061 acaatcgatc agataaagaa ctagctttag atattcagaa aattttaatc aggaggacat
    18121 agtatttgag gacagtaata ttaagaaata aattgaatga agaaattgat ttgtccacag
    18181 aagattattt tgctactgaa atgtcgaatc taggttttga agttacaaaa gagcatattg
    18241 gacaatgggg gagtttcaga gaaataggag agagtattga attgtctgaa tttcaatcat
    18301 cagttatgat ttctgttcac ggatttagag aacaacaatt atatggttca ttagttgaat
    18361 ttctggctaa tggacctttt gaactagaat tttcttttga ttcagaaaca atgatcagga
    18421 aatgtagttt gaaatcatta tctaaaacag aaattgattc ccaaagctca ttgctaacag
    18481 atactttgaa tctatatttt acttcaagct ggtattctat taagcgtgaa aaattaatac
    18541 agaattcaac ttctctcaat acacgtggaa aagtttactc atttgttcga ccatatgtct
    18601 acaccaaaaa tgtatgggaa aagaaaggtg tatttaagtt cagtaataat tctgtatatt
    18661 tgacaaatag tacagagcga atgtctccgt taaaaataag gattattgga gagtgctcaa
    18721 atccatattg ggaagttatc caaaactcta aagtagtagc ttcagatggc tattttatta
    18781 atatgaaaga cacacaaata ttagaagtgt ctagtttgct agaagaccaa acggcaattt
    18841 taaaagatgt aggtggagtt gaatcttctg tttatcaaca acaagatttt actaaatcaa
    18901 acttcgtcca agcgcctcta ggagaattca gcgttgtttt tcatgttgga catgcaaatg
    18961 tagagattga actttatgag gagcgtgatc tattttgatt ttagccatta ccctctttca
    19021 ccgagatatg aatttatata acgaacgttt aatttcgact gagttcgaat ttggaacgga
    19081 tgaaattgga gaagaagtaa gtaattttat tacagataaa tatgtccatg taaaatcagg
    19141 agattttcta ctagcaaaat ttattcctag cgggaagatt gcttattttg gagtgattag
    19201 ctcgcaagaa gacaaaaaaa taaattgcaa aggattactc agtttggccg atagcgagat
    19261 accgactgta aaaatatcag gtgctaatta tgaagagcat attcgacgac tgattgaata
    19321 ctacttattg aacgatccaa caaaacaact aaaaaatatt ttggaggtaa aaacagaaag
    19381 cgttacttct cattcttatc aagctgttga cacaaataag agacgattga atgcatatat
    19441 aataaatggg ttcaaaaaat ataatgttaa atggtatgtc aaagagatta aaggtaaaaa
    19501 aatatatact ggtattcgag cagttgatga ttcaatttat attaaagata attcatctga
    19561 gtttaatgac tgggatgttt ttgtgcaatc tcctggacca gggaacgaga acaaattatt
    19621 gatcgtagat aaagcaatga aagatataga aaatcccttg gtattgtcta cgtggtattt
    19681 agatgacgaa aataatctta cgcaagattc aacgaataag aacattgcat tgcctaccgt
    19741 taatttagta aatatatatg atcaaactca agaagataga ccttcttatg aagaggtagc
    19801 taaatcggag cttaaaggca acgcctattc acatgaaatt aaagttaatg ttgtgctaaa
    19861 tgctagaaat atcaatgtag aaaatatcga aacaggaatg ttagcgacta ttgtttacaa
    19921 agagcagact tacaaatctg ttctaactgc ctggcgaata tccagcagta aaaagaatat
    19981 agaattgaca tttggtaatg tccgaagtcg ttttatggat tattttgaag ataatggggg
    20041 ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag taagcgcaga
    20101 aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta ttgatggtta
    20161 cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg caggagcagc
    20221 gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag ttccagcaaa
    20281 ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac ctggatctat
    20341 tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag aatttataac
    20401 aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac cactatgctc
    20461 agttaattct actggatcaa ctgtttcaat ctcaaagata tcggatagtt acgagctgac
    20521 tctctctaaa ggagaaattt tgtggagggg gactgcgtta atgcatgata ctcaaactgt
    20581 ccaaccttca aaaaaaattt ggcagacagt ttcaggtttt ttgttagtat ggctcccata
    20641 cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata aggagcgtat
    20701 aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc acaaaaagtg
    20761 gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg cagcaagcaa
    20821 tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt agttaggagg
    20881 tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca gccttatcta
    20941 cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa tgtacaactt
    21001 cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt gaaatttgtg
    21061 ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga ttcgactaat
    21121 gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca attcaaacaa
    21181 gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa cttcatcata
    21241 gacgtgttag aacgagtaga tattaattcg gagcaagcga aatactatat ttcttcattg
    21301 gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga taaacaggtc
    21361 caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa tgaatcagat
    21421 aagcaagtaa atgatcgtat tgataaggca aataatcgta ttgatcaaac caatcagcaa
    21481 atcggcgatc tcggcaagct gaaaaagatg tacagtaaca gcattga
//
LOCUS       DAJSBM010000044        21503 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJSBM010000044 DAJSBM010000000
VERSION     DAJSBM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.044"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(239..1702)
                     /locus_tag="ONT67_001834"
     CDS             complement(239..1702)
                     /locus_tag="ONT67_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2355572.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1766..4783)
                     /locus_tag="ONT67_001835"
     CDS             complement(1766..4783)
                     /locus_tag="ONT67_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2355573.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4934..5773)
                     /locus_tag="ONT67_001836"
     CDS             complement(4934..5773)
                     /locus_tag="ONT67_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2355574.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5918..6163
                     /locus_tag="ONT67_001837"
     CDS             5918..6163
                     /locus_tag="ONT67_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355575.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6226..6648
                     /locus_tag="ONT67_001838"
     CDS             6226..6648
                     /locus_tag="ONT67_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355576.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6742..7059)
                     /locus_tag="ONT67_001839"
     CDS             complement(6742..7059)
                     /locus_tag="ONT67_001839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355577.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(7086..7292)
                     /locus_tag="ONT67_001840"
     CDS             complement(7086..7292)
                     /locus_tag="ONT67_001840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355578.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7273..7455)
                     /locus_tag="ONT67_001841"
     CDS             complement(7273..7455)
                     /locus_tag="ONT67_001841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355579.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7469..7819)
                     /locus_tag="ONT67_001842"
     CDS             complement(7469..7819)
                     /locus_tag="ONT67_001842"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355580.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7845..8147)
                     /locus_tag="ONT67_001843"
     CDS             complement(7845..8147)
                     /locus_tag="ONT67_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355581.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8150..8383)
                     /locus_tag="ONT67_001844"
     CDS             complement(8150..8383)
                     /locus_tag="ONT67_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355582.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9369..9691)
                     /locus_tag="ONT67_001845"
                     /pseudo
     CDS             complement(9369..9691)
                     /locus_tag="ONT67_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9814..10929)
                     /locus_tag="ONT67_001846"
     CDS             complement(9814..10929)
                     /locus_tag="ONT67_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2355583.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10935..11333)
                     /locus_tag="ONT67_001847"
     CDS             complement(10935..11333)
                     /locus_tag="ONT67_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2355584.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12362..12748)
                     /locus_tag="ONT67_001848"
     CDS             complement(12362..12748)
                     /locus_tag="ONT67_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCR2355585.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12745..13266)
                     /locus_tag="ONT67_001849"
     CDS             complement(12745..13266)
                     /locus_tag="ONT67_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCR2355586.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13268..14302)
                     /gene="sppA"
                     /locus_tag="ONT67_001850"
     CDS             complement(13268..14302)
                     /gene="sppA"
                     /locus_tag="ONT67_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR2355587.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14335..15750)
                     /locus_tag="ONT67_001851"
     CDS             complement(14335..15750)
                     /locus_tag="ONT67_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR2355588.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15719..17872)
                     /locus_tag="ONT67_001852"
     CDS             complement(15719..17872)
                     /locus_tag="ONT67_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HCR2355589.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            18130..18366
                     /locus_tag="ONT67_001853"
     CDS             18130..18366
                     /locus_tag="ONT67_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HCR2355590.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18385..18600
                     /locus_tag="ONT67_001854"
     CDS             18385..18600
                     /locus_tag="ONT67_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HCR2355591.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19303..20055)
                     /locus_tag="ONT67_001855"
     CDS             complement(19303..20055)
                     /locus_tag="ONT67_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355592.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(20070..21317)
                     /locus_tag="ONT67_001856"
     CDS             complement(20070..21317)
                     /locus_tag="ONT67_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2355593.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(21393..>21503)
                     /locus_tag="ONT67_001857"
     CDS             complement(21393..>21503)
                     /locus_tag="ONT67_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCR2355594.1"
                     /translation="SDQELKKIKGGAGTKPQGKPASNLVECVFSLFKKCN"
BASE COUNT         6344 a         4108 c         3166 g         7885 t
ORIGIN      
        1 aagcaattac tgatttttaa attctttata aataaaataa aaatcgtttg agtaaagaaa
       61 taagtcaatg aagacccaaa cgataaaaaa tattcctaat actatattta ataaactacg
      121 ttctagatag atacgagatt tcaaattaaa aaacgaaaat gagagataca aatatatcaa
      181 atatttaaaa agtaaccaca ttttctatac cactccttat cagaaacagc cttattcttt
      241 agaataagac tgtttctggg ttgattaatt tgtcttttgt taaactacca tctaaggcaa
      301 gttctaaatg taaatgtggt cctgtactat ttcctgttga acccacataa ccaataattt
      361 gtccttgttc aactgtttgt cctacgttca cgcaatattc ctgttggtgc gcataaagag
      421 ccacacttcc gtcttcatgc tcaattgtga cataatttcc tgacaaagta tgatacgaag
      481 cttctatgac cgttcctgcc ttgctcgcat agattggttc tccttgattg gctgccatgt
      541 ctaatcctct atgaaattct tctcctcgcc aaccgaagga actagaaatt gaataatttt
      601 ttagaggtac catatagcct tctttgctta gttgtggacc agctacttct ttatcatatt
      661 tgattaagtc ataggtttcg attaacccat ttaatttttt atcgtagcta gtgtctgttg
      721 cgtatcttcc tgttaaaaat ttagtcgctt cctgataggt ttttgcctta cttttccaaa
      781 caccatcata aaaagaggta tttccaacta agccttcttt taacagtttg gcatagtctt
      841 ttaagctttc ttcatagctc tcatattgcc gaaaagttgc ttgggtcgta taaagtgtcc
      901 cattgcctaa atcttcttgt gtcgcaaaag agacgccctt tccgttatgt gtgcctttga
      961 ttccaaatag attgtagttt ggtgcctgag ccaattgact ttgtccgctt gcggattcta
     1021 aaatggcttg cgcgatcata acagaagcgt ataaatcatt ttcttgtcca attttacgag
     1081 cagattcacc gattttacgg ataaacgttt ctacagacgc gtctttttca aaatgaatag
     1141 tcccttcgtt atcttgtgtg ctggaaggga ctaattcttg tatcggtgcg cttggtgctt
     1201 ggtggtagtt ttttgtctct tctgaaccga ttgattgtgc ctgcggtacg tcattcatag
     1261 gtacagtcac actctgttca ggtgcctttt gtgggcgttc cgtttttgtt ggcggttccg
     1321 cttctgtttc ggtacttggt ttttgaggag tctgtgagct ttcatcagaa ggttgctcat
     1381 ttggttcttc tgacggttcc tcctcaggtt gttgattgtc ttctggtttg tctggttgat
     1441 tcggtgtttc tggttgttct tcagaacctg ttggtggagc gttcatttct ggattatctg
     1501 gtgtatctat tgtatcttca gtcggtgttt ctggttgatt ttcttctcca gtggaatttg
     1561 ttgtcgtttc ttcagtgatc gttgcagatt gcgaatttgt cagttgattg ttgccttgtt
     1621 cattagccaa aaccgctggc gtatacccaa atacttgacc tactaataac aaagcagata
     1681 aacatttact cgtttttttc acaagattgc ctccattctt caatcaatca tctagagaca
     1741 gctgattgct gtctctagtc atactttaat tgctttgttt cattttacga cgaaccagaa
     1801 cagcccccac accaacaaaa agaaacagtc ccaatgcaat taacacatcg ttcactgttt
     1861 ctcctgtttt tggtaagctt tgttttggtg tgctcgtcgg cgtttgtttg acaggggttg
     1921 tcggcgtttc ttttttaatg ccttttgctg tcaatgtttg tttttcattc ttcaaatcaa
     1981 agtgacgtgc ttcttcctct ttatggtctt tatccttata gacaatatga gttgcgacca
     2041 atttctcccc gtcttttaac gtgcctgctt tgtagtcaaa ggatacttta aactcttcag
     2101 acgctttaga aactttacgt gcagtttcta ccgtaccaac gactgtatct tttccatttt
     2161 tatcaatctt atggaattga gtcacccagt atttggtttc ccctaccgta gattctggaa
     2221 attcttgcga tacatgatcg atcaactctt ggtctttgtc tggatcaaat gtttggtcgc
     2281 cattcactgt aataaagcgt gtttcgattt ttggttcttt cgcagtcact gtttgatctt
     2341 tattggttaa atcaaaatga cgagcgaatt cttcttcctc ttctttgtcg ttatagacaa
     2401 tgtgcgttgc gacaatcgtg tcaccgtctg aaaccatgtc tgctttgtag ttgaacgtca
     2461 catcaaatgt ttctttttca tcggtcactt ttcgtgtttc ttttaccgta tctaatacag
     2521 tgacttttcc atcggcagta attttatgga attgcgtcac ccaatatttc tctgtcccaa
     2581 ccgcagattt agggaatact tgggacactt tatccactaa ttcttggtct tttttcgggt
     2641 tgaaatcttt tccacctgtc gttgtcacaa acaaagtaga aatttctggt tggtcttctt
     2701 ctggaaccac gatttcttcg tttttcacag taattggttg tacttctccg tctttgaccg
     2761 ttactaaaat cgtttctgtt tgtcctgacg cttgcccata atgctcaggg aacacaattt
     2821 ccgtaaattt atacgtaccg tctggtaatt ttagtttttc tggttcttct tttgtttcat
     2881 acacaaagga cacattttta ttatgtgcag tcataccttc aatcaggaat ttcgctcctg
     2941 gtaattgtag atgattgatt gaatcgactt tattgaagtc tacttcaccc gtttttaaca
     3001 agttgtcaat cacatctaat tcaaacgtaa gcacaggcgt cgtgttatcg ccataggcca
     3061 cagagaacgt ggtttgttct tcagaaggtt gacggtagtt gtctggcaca tttttttcaa
     3121 tcaattggta tttgttttga tcgccattgt agaatttttt gtttgccttc cagtgtccgt
     3181 ctttgtcagt ttttactgaa tccaagactt ctcctttttt cacaaggact gtttctttat
     3241 ctggcgcagt aatatctgtt ttggcgataa tatcaaattc gatattttct gcaggaacgg
     3301 tatcataaac aggtttgcct tgattccagg attcttccgc attatagact tctttgactt
     3361 tctttccttc tggaatgaac tgttgtaagt cattttttgc agttaacgtc acttctttgt
     3421 ttggttcaat cgtaatcgta tgagtttgtt tgtctagaat atacccagct ggtgctttga
     3481 cttctttgac atccaattta ttttgaacat attttccgtc tttttcatag aatgcaggga
     3541 tttcttttaa ggcgatttcc cctttggcat ccgttgtata ttgttttgtt tctcccgtat
     3601 catgacgttt gatttcatac gtcacatttg gaactttttt ccctgtgaca gcgtctagtt
     3661 tggttagttt gaagtcgcca ttttttagaa ctttaatagt tagtccagct ctattaggat
     3721 ctggcacata aagctttcct agattttgag caacaggatc tacataggca aaacttgaac
     3781 ctacccctct agaatttgga tccagtgaag aaacagaaat tcgtccgtca tttgagtttt
     3841 gatttgcttt tataaacaaa ctatttcctg attttcttac ttgaacgtta gctgtgtttt
     3901 caacaaccga cccatcccat tgagaaaaca caccttttgt atccttaact tctacttctt
     3961 cccctacttt aaccgtcact gtttggctat caaaagaagg atcttcaagt aaattgttta
     4021 tttttctatc tacagtctgt ttgaaagctt cataattaga ttgtccaata gttccataat
     4081 taacaaatga accacctaaa acttcccaaa tatatgtttg tgctaataca tagttatcca
     4141 ttgaaccact agaatagtag ccaaaataac caattaaaga aatctttttt ctaatagcac
     4201 ttgtcgtaat taaattttcg agtggtgtca catcatattg aatacccgca ctcgtgattg
     4261 cgccttcttg aatgcaaaaa acatcttctc cgtcaatttg ccagcgaacc atgtaatcat
     4321 gagtatacat tgttccttca ggagtaattc gagtcatatt cacatcttta cgaccatttt
     4381 cagtcaatgt tagattggta gcacgtgtct gtgcattctt ttccaagatt gcttgccatt
     4441 cttcagattc ttgcgccaat cgttcttgtt cagaaaaaga agtcgctttt tgaatatctt
     4501 caatggttat aaagtatttc tcataacctt cagggacctc ttgagtcatt acattgggcg
     4561 gagaaacatc ttctgtcgtg tcctgcgtag ttgtctcttc tgttgtttca cttgtggttt
     4621 cagtactgat cgcttcttca ctactttttg attcttcagt cttcattact tcttcagtag
     4681 aatttgattc atttatcgtt tctgcaaatg ccgttacaga tcctaacatg ggattgagaa
     4741 atgtaacgaa aattgccaac caagttgcca ttcttttaaa catatttttt tcgttccttt
     4801 cattttattt ttcagtcttc taaagtaatt tcttatggac ttcgacaccg aaacattttt
     4861 atcctcctca aaacaaaaaa agactagaac ttataattct agtcttcatc aaccacttac
     4921 ttttctttta tttttagtag aaatctacag tgtgtttttg tgacccgtca ctgtaaataa
     4981 taacatccac cataaaacct ttgttgtacc actgcgaatt tggatcgcta acctctgcat
     5041 tagcccaatc ataggcttca tctacagtgt taaatacctt tccactatta ccaatctctc
     5101 caactacaat tgttggctct tcaggtgtcg ttgtgccacc atttccgcct tgatcgcctc
     5161 caccaggatt gcttggttct tcaggcgttg ttgttcctcc acctgtgctt gggttagaag
     5221 gagttgtttc tcctccagta tttccaccat tacctggttg tgtaggtgtt tgagtagtgc
     5281 caccattgtt ttctggtgta cttggcgttg ttgtaccacc agttgttcct gtttgatctc
     5341 cagctggtgt tgttggtgtc gttgtatttg gtgtcgtttg tgtatttgaa gtcgttcctt
     5401 gtcctactgg ttgattcgga tcaactttgt ctttctcttg aatcactgtt tgagccacag
     5461 tttcacctgc gctgttttgt acacttgcta cattgccatt aaatactaat ttatttcctg
     5521 catagattaa atccacattt gtaatcttat tcatgtccgc taatttttgg acagtaatat
     5581 ttgttgcttg actgattgcg cttagggtat ctccccagac aattgtgtat tcattccctt
     5641 taatttctgc tttgatttgt tccgccgaac gaggtgtcca aactttgttt tctgctgctt
     5701 cagcattcgt tcccacagca agactaactc ctccgactac gattcctagt gccaatactt
     5761 tcaacatttt cattgttttt cgcctccgat ttgaataaat ttatttacaa aaacatagta
     5821 ccaaacttat gtctatcagt cgatatattt tttgtagatt tttttatttt ttgatatact
     5881 aactttggta ctaaaataac taaaaggaat gtcacacatg caacttaata atttaaatga
     5941 aatacatgat tgggctcaaa ataatttact taatcgtaaa caagctgcca aaatcactgg
     6001 acaaaactat gatgcttttt ctagctcttt acgtagaaat aatatcaaac catttctaga
     6061 aataggcgac gaacctggtc cttccatcat tcgcttatat ctaagagaag atattgaaaa
     6121 atacgcaaaa cagctcgaag aaaaaagaca gaaacgtcaa tgaggcgttt tctgtcttgg
     6181 atttacaagt aaaacaataa taaacacttt tacaacggag gttctatgaa taagaaatac
     6241 attgtttcag ttggttcaat cgtactgatt gtactcgcta ttattgcttt ttctgtatcc
     6301 aacaaaacag aaacacagcc acaaaatacg gttcaatcga ctactacaag tcaatctagc
     6361 gatcattccg tctatccttc actaaatagt ttaaaagaaa cgcatggaca agatttaatt
     6421 gatcttggtt cttctcaaat tactactgtc ttatttgaaa agaaaaatat tgcgtttatt
     6481 actcctaaag aaggaactga ccttgaattt tctatcaaag taacaaatac caaagaaaat
     6541 ttattctcca ttcctgacat tcaatatacc cctaacaagt taaagattgg ggatacgttt
     6601 ggtttggcta aagtaaatga acaatacttt gcttactcac tgaaataagt ttctaatagc
     6661 aagaggaaaa accctcttgt tatttttatc acataatcat tttataaaaa cgcttcgaaa
     6721 atatcaacat ttttgaagaa actatttttc taatttctca atttcttcca agggattttc
     6781 gctaatgacc catacaggca cagattgctc tcgaggctcc ccttcttcta accaccaacc
     6841 tgcataatga acttcttcct cctcagcatt accatgatac caaccaacaa aggattcatc
     6901 tacaacgaat gaaatttctt cttctggatc aaataattca tttttccatg cttcaattgc
     6961 ttgttctttg gtgtactttt gtttgttaaa tacaccacaa atttcgtcat ctacataaaa
     7021 aatttcaaaa tcaaaattac tcgttccttt tactggcatc ttaacttttc ctcctttgac
     7081 tcacttcaat agatatacca ctcatgttca ttccattgtt tactagttaa ttggagtttt
     7141 atcttagggc tttgtgcact ctttccaaaa acttgagcgt cgtttttttc ttctaaccaa
     7201 aaaaggtaat ttcttacatt cggtactaaa tgtttttcct gcatttcttc atgaaaaaca
     7261 tataattctg cttcattttt caaccaattc attattcatt ccctcattca cttcttcata
     7321 ccgccattca gatagtgaga tttctttttg tttctccact tgtcctataa aaatcgctgt
     7381 attcaacaag ctgatcacac ttaataataa agcaatgaaa ctaatggtta cacttactct
     7441 ttcacatttt gtcattttgt ttccttcttt actcgtttgt tatttcttgt aataggtcta
     7501 ataactcctt caccgaaatt tgcaaatggt tcgcaaaaat ttcaataaaa gcactcgcac
     7561 acattaccag accttcaact gatatatcac tgcgacaata aaattctgga gaaacactga
     7621 tttctgattc tttcttacaa ttgaacgaaa aaactatttt tgaatcaaat tccggtaatt
     7681 cttccacaac tatgttttgg gattctacca agaaagggag tgcctgttct aatgcttctt
     7741 ctggtgtttg agcagataga ataattgctt cttgttcttt tatttcattg ttctcaaata
     7801 ctttataact ttttttcatt gttagttcct ctttcattcc tctctcaatt agctaagccg
     7861 ttactggtac gccattctct aaatttagta tcgctaatcc ttaatgctcg tcgaatatca
     7921 atcattcgat aacctattgc acgaagttct ttatatcttt tagcagttag cgtctctaac
     7981 gtcatttcag gcaatgggtt cgaatcaaaa atagaattcg aaccgattat ttgggccaat
     8041 tgttgtactt gtaacagctc ttctgaactt aattcagtga ttgctcgatt gcctatcatt
     8101 gcatacattt tctgtcgaat atacttcttt tcttgtttag acaacatttc tatccactcc
     8161 tattttcatt atcatttgtt acctccactt cagtaaatcc acaaataaat gaattcatat
     8221 tccaagtacc attcccttca actacaactt ttctcctgtc ctctttcgga tattcaagaa
     8281 tcagaccgtt taccaatatg tttttcacta ctactaattt gtttgtatac cgtggaattg
     8341 ctgttccgat atacttcact ttatttccta cgctaattgt catttcctca ctcctattct
     8401 tttttatcca aaactttttc ctgtcctatt cctgccaacg aatatacttc actgcatgct
     8461 taaacaaaac aaaatgttgc cctgttttat ttccgtcttt tacttgttcc acatacagtt
     8521 catatttggt cacacctttt aaaacgccag atactttttt tcatccattg tcacaattag
     8581 ttttttcatc aatcgattga tactgcactg ctcatttttc ttgttcatat agtattcctt
     8641 tctttttcaa aactagcctc ttttgtttac tagttttcat caatcatcaa cgatttaaca
     8701 gattttttct ttcgcagggt ttctagcgtc agcaacccca aactctaaaa aagtgccttt
     8761 gctcccttga aaaacgagcg gacgattgta ctcgtcatca tatgtttttc atactacctg
     8821 atccacgcaa ataacctttt tttgttctgt gccacaccca tccatacgta tcgcttgaga
     8881 ttcaccatac ttttttaggt ggtttatcct tcctaagtcc ctgttagaca cgctgattaa
     8941 cttagaaagt cctgtttgta tccaattttc aaagatctcc caaaagaaat aagtaggttt
     9001 acgtgtatcg cttctgttca aagtacctgt ttgtacttga gtatttctac tcccctcgtt
     9061 caagggattc cttccaccag ctagcttttc ctttcatcat caaatgttcc tgaaaagcat
     9121 ttacgtttcg tgtccgtgtg cttacttatt cttttttcga tagcgattat ccgctcgcta
     9181 ctcgctattt tatactttgt ttgttagctc aaataaaaac taacaaacaa aaaaggctag
     9241 tttctttccg ttttattttt ttaactgtcg ataaatagtt gttagtttaa ttccaagttc
     9301 taaaaccaat acgcccaaca aaaccacagg aaaaacaatt ggggcaaaca agtaaaatcg
     9361 gatagttttc atgtcttccc tctttctttt tggtgtcgct gtggtcgttc ttgccgttca
     9421 atacttcgat acatttttta ttcattattt gctcgttttg ttttcctctg gactgctttt
     9481 cttgcttttt gaatacttta tacgcttttc gtgcctgaat taataggtct tgtcggcgtt
     9541 tcattcgtga gaaaacaata tttgaaacca tcaaatcctg gttcgaacaa ccacgattcg
     9601 caataatttt cccatcaaaa aaaatagttt tcacttgaga aaaaaacagt tggaaatctt
     9661 gttttgttgc taattttttt gtcaaaatca ctgtgctttt ccctgttgaa tctacaatga
     9721 ttcttcgaac aatccgattc gataacttcc aacgttgccg attaaacaat gttacttcac
     9781 aacaaatatg acgtaagtga ttaggggtca ttttcatgtg tccaattcct ccaaccaatt
     9841 atgcatcgaa attttgggca attgtgacag cccataaatc tgcgtttcca ggtcttcata
     9901 actacaagaa cgcttggctt gaagtaatga atattctgct tgatctgata gttttgttgc
     9961 cattggatct agattcaatt ctcttacaca acattcccaa aaattcaata gcgctctaaa
    10021 ataaaatcct tttcgattct gaattgcttt tccttcatga cgagcaattt tttcattttg
    10081 aatacaccgt tgatacgttt tgcttaaatc ttctgcaaat gtttccccta aaattcgata
    10141 ctcgcttcca tcagataaac catgtgtttg attatacttt cgttgccgct tttctataat
    10201 tcgtttactg tcgtatatca tgtttagctg ctctttcgct tcacgccatt cagctgaact
    10261 gcgaacaatc catgtcaaaa catctttcgg aacaaacgta ctttcgctgt aatgagcaat
    10321 aaactctttt tgccaatcat cagccatttc aacaggacta gtcgtatcga tttttgtatc
    10381 tagattttct gtatctagat tgttagaaga attattaaat aattgtgcag aattttcaca
    10441 tggatttttt tcgtcaaaat catcgaaaat cgctaaatta tcaacagatt cctcctcttc
    10501 ttttccttgt gcagaatcta cactggttga gtcaacagga cctaatgaat acacatcatt
    10561 ttctgtcgta tctaattctc ctagatacaa ccgagctttg tttctttttc ctgtttgttg
    10621 atcaaatccc atatcctcta tttctagtag ctcatattct ttcaattctt ttttgattgt
    10681 attaacagaa cgtctgcaac aattcaatgc attggctaat tcttgttccg taaattcaaa
    10741 ataaacaaaa ccttgactat ctacttgatt tcgttgaatg gctaaatcta agcgtgaccg
    10801 aaaaaccata tacgcgattt ttgctttatc agacaatgag cgatattttt cacttgtaat
    10861 aaataccttt ggaaattgat agtataattc attgtattta cgagttgcgg taaatctttt
    10921 tgttgccatc gttattacac ctcatcttgt ccatatatta agaaagaatt ttggtgttgg
    10981 tagacacgaa catcttccat tgtagcaatc aattctttta gataaattcg accgaattca
    11041 attgttagta agtcgtaatc ttctaattca tttttgattt cttcaatttc ttcttcagac
    11101 acattgagta aaatggctaa ttcttgattg gtataagaaa tatagacatg tttcttttca
    11161 tcaatccgtt tttcaattaa ggattcttct aagtactcat tcagtaagct ataggcgtat
    11221 tttgcttgtg cagtcatcgc ataatatttt gacgatttta taaagaatag ttttggcata
    11281 tgaaaagttg cttgtgtcag cacatctgtt gcgcgaaatt tgttttttac catatttttt
    11341 cctcctagaa aaacacgcaa aaaaagctct gtataaatcg aattacacag agtttcttct
    11401 tgttgatatt taatttctaa aatacgtttc gggtaaaaac taaacactta ctaatattta
    11461 gattgtagaa tcttcgcctt catttattca ttttaaagag aaaaagatca agtgaaatgt
    11521 gcccaaaagt ttgattctat taatttttaa acaccattca taatatattt cctataatgt
    11581 aaaaagcgtc gatacttatt aaagcatcga cgttattttt atagtaaatt tatcaaaatt
    11641 tttgccaaca ttttttccga tttttttccc tatttctatc tttccttatc tttcttagtt
    11701 aatattattt gatgttaata tatcactact aatattaaaa aacattgatt tatcatagga
    11761 taaagagtga tagtttaata cttaaagaga catattaatt ttatttaaaa attgacatta
    11821 atgactctta atcagcgggt cgcgggttcg agcccctcac ggcccattgg gtgccaaacc
    11881 cacggaacag gtaaatgttg gtgcgaaagc atttcgaaaa catttacgct gttctttttt
    11941 tatttttcaa aataactagg gctttttatt tttaactcaa agtccactgt agccctttgg
    12001 actttgagtt aaaaaagaag cataattatg aacagtaaag tttctaatat aacaataatt
    12061 cgagaagctt tgcaaaatca tgctgccaat gaactctcta aacatatagg attaaatcta
    12121 agtactataa aaggttaaaa agtgaagaat atttaataga aaaactaaat ttacttgctg
    12181 ctttcccctt ttccattaat acaacaatca gagagtgtct ttttttcgaa aaaattagaa
    12241 taaataaaag acacgtagtt tttttatcta catgtcttcc atcgactaca agttatcatt
    12301 tctcaaaacg tatttagctt ttaattgatt aatcgtttca cgttgtctac acattgccta
    12361 attaagattt tcttattaga agcatcaatc ccgcaataat acctaaaact ccaggaatta
    12421 cccacaataa tgttaaaaga aaaaatggaa taatagataa aatgagtaaa ctaataccat
    12481 agaccactgt tttttgattt tttcttacta aaaaacctat tatgaaaatc attatcgaaa
    12541 aaactagggt aattcctcca aataaaacaa cattcccgct tagattgact aacatatcta
    12601 tatccttact ggttagttct gtagtattta aagtagttga atcaatagaa gatagaaaca
    12661 taccacccat caatgctaaa agactacata aaatccctgt tattccacta attaaaatca
    12721 aaataaactc tgtttttctc ttattcattt aattctctcc agattaagtg ttttagaatt
    12781 agaaattttt tgaccatagt taatagcttc taatgaacga ataacaacca ctgacgtgtt
    12841 agaaaaataa tccccaatat gttgtttctt gggcgtgaaa gcagccacca aatacccaat
    12901 ttgaaataca aaatttttta atataaacct acatatacac tcacgaataa agatagtttg
    12961 ccaacttagc tctttttcct caaagctcac tactcttatc ccaaaaatca ttttcccaat
    13021 tgtttgtcct gaattcagct tagtcaaaag tacaaaatag gttaaataaa tagctaatga
    13081 cgctatacca taaaaagtaa taatggattt gtcagattcc agtcccatga ttttgtaaaa
    13141 gagacctata gtagcatggg tgatgatgct aatacaaatt aaatcaataa gaaaagcaaa
    13201 aaatcgtaac caaaatcccg caaatacata attaggcaaa gtttcaatgc cactcttctt
    13261 attcatttta tagccctcca tagtaataca ttggttttgg agtatcagaa aatccaagtt
    13321 tttcaaaaac tgaaattaca cgatctgatg tagttgcctt tagcccttgc cattcagcaa
    13381 tcttattacc taaccaagaa gaagcaaagc cagttgtgtc atttttatat tctataaccg
    13441 tcgcatcagt taatttcttc tcttttttta gggaatctaa tgcatcttca gagtaaccaa
    13501 tttgatccac caacccattt tctactgctt ggttaccatc ataaattctt ccatccgcaa
    13561 gttctttcac tgcattagta tccatatttc ttccttttgc tacaacattg acaaaacgat
    13621 cataagcact catcacaaat tcctgtataa ctttattctc ttcctctgaa ggtttgtgtt
    13681 gaggtgacat catatctttt aatgcaccac ttttatacgt cgcatcagtt acgccaagtt
    13741 tttctaataa gccagaataa tttaaaccag aaataatgac accaattgat ccagttgttg
    13801 tttcttccgt agcaaatatt ttatctgaac cagcagaaat gtagtaccct ccacttgccg
    13861 ctgtattttt aaatgcagta tagataggaa tatctagctt tttaattttt gccatttctt
    13921 tagcaatctc agcactttca tatattcctc ccccaggtga atttacttct aataacactc
    13981 ccttaaccgc tttgtcttct tgaattttct ttagctgtgt taaaaaattc tgatggttat
    14041 attgttccct actaaaaaga ttgctttcac ctgtatctgc aataactcca ttgacggata
    14101 gtttgacaat ctttttattt gatgcgcctt cttctaacgt ttcttctgtg atttcgttag
    14161 atccgtaaaa aatcttattt attcctgtta gggttgtatt tgatgcttcc tctttatctt
    14221 ttcctggaat tagcgcgacg ataatggata gtaacaataa tccgcacgct actcccacag
    14281 caatccatct tcttttattc atataaactc ctcctatatt cttagtaaaa ataattattt
    14341 tttatttaga aacacatcga caatttgttg ccaatatgtt ttttctccta caattaaaga
    14401 caactctcca gttaatccat aacgaaacgg aaacgattga gttgttttta acgttccttt
    14461 cactgcataa aaaacacctt tctcagtggc agtagaattt tctgaaattt catctaaagt
    14521 tccgtcgatc gtaatcggag ccccaccttt ttgatccaat ttaaaatgaa ccttcatccc
    14581 ttctttaata taggtacttt cattcgcagg taaaagtgct gtaaatttca tttttgattt
    14641 ttcgtggatc ggataaattt ctgcaagcac tgttcctttg gctagttctg cttgtccctt
    14701 tgtttcttca tttaaatgaa ccgttccaga tacaggagct tttagtattc cttttgaaag
    14761 ttgatcgtcc aaacttttga taactgactg gttcttctct tgtttttcat ttagctctgt
    14821 aactgtttgt tttacagttg ttattgcttg ttctagctct tgctttcctt tttccgtttg
    14881 actaattctg tcattctcag aagtagttgg tttaactaat ttagcttttt ctgactgata
    14941 ctgttctact tcttttttta gttgctcaat ttgttcattt atgcttgctg aaattgtcag
    15001 tttcacttga tttttttgat catcagaaac attatttagt tgttcttgcc aattctcata
    15061 gtttgccatc atttcttttg aaaaatcttt taatgattga ttattactcc aagctacctg
    15121 tacttgttgc cattcttgta atttactatc tgaactctca atttgttttt ctagttgtct
    15181 ttttgtcttt tcgtatactt ctaattgctt ttgatcattt aattcacttt gcttcaaagc
    15241 gtagcggagt gattcttttt ctgacaacat gctctttaat tgattactgt agccaaaact
    15301 gtcatccgtt gaaaacaaat tttttccttt acttatactt tcaataaata gctgagccat
    15361 ctttatttgt tgagtaatac gttggttttc ttgttctatc tgagcctttt cattctctat
    15421 taaagaacaa tccaatctaa ccaacacatc gtccttatga acaaattgat tttcttttag
    15481 gaagttttcc attattttag cttctaatgg cacttgtaac ttctctattt tttcggggtt
    15541 caattgagcc gttgtccgaa taacaacctc ttttttagca aaggttaaaa acaatccaat
    15601 aatacataaa aaaagaatta cagggtataa cacccaacgg tagaagctat gatgttgttt
    15661 agtatagata ctagaatgtt cgaaccaatt attattctgc ataaattgtt tccctctcct
    15721 agatttccca taatttttga tagataccat cggcaaaacg aagttcatcg tgcgttcctt
    15781 tttctaccaa atgcccttca tggagaacta aaatttgatc acatgctttt gctattggta
    15841 aatgatgcgc aataaaaata atcgtttttt cttttaaatt gaggagataa ttcataattg
    15901 catgttctaa caaggtatct aatccactag ttgcttcatc taaaattaat atgtccggat
    15961 tttttaataa agcccgtgct aaggctaatc gttgtcgttg ctctcctgaa agattatttc
    16021 caccttcttc caacaatgtc tcgtaacgta atggctgttg attaataaat tcatttagtt
    16081 ggactatctc acaaacttct attatttgtt cgaaacttgg tgggctatct aaaccgaata
    16141 taagattctc aatgattgtt ccactaaaga aaaatgactc ttgaggcaaa tatgtcactc
    16201 gatcgcgtaa tatctgaaaa ggaatgtcta agtgattaat gtttccataa tggatcgatc
    16261 cttctgatgc ttcataaaaa cgaacaaata actttgcaag agttgatttt ccagaaccac
    16321 taactccaac tatagccgtt ttactgtgcg aaggaattgt acatgaaatt tcgttcaacg
    16381 ttggggcctt catgctatac gagaatgaaa cgttttttac agtgatatct tgttgaaata
    16441 attgttcaga aagcatatat tcaaaagact ggttcgattt ttctgaatca atcgcgaaaa
    16501 tttcatttaa acgattattt gcaacttgag ccgtttgcat tttcacttgt aaattaataa
    16561 tgttttgtaa aggatctgtc aaaaatacta gtaaagcatt gtatgtgata agttgaccta
    16621 gactaattct attatccata acaaaataag aacctagcca taaaattaat gcactgctaa
    16681 ttaattgaat caaatgttta attccttgtt gaatattatc aagcgtaacc gtacgaaatg
    16741 attttttcat taatttaaca aattcatggt ctatacggtt atatactttc tcttcaccgc
    16801 tatatgcttt aattgtttct attcctttaa gactttctat gatactagaa tttacagctg
    16861 caccggctgc catttcttcc ctgttcgatt tttcatagct tttcacgaaa gcgaatattg
    16921 caactacata aaaaggcaaa gagcatagag tgattaaaaa caacgtacta ttctgaatag
    16981 ctaacgtaac cccaacaagc aacaccatac caatatctaa aaatattgat aacgtagcac
    17041 ttgctaaagc atcaataatc ttactggcat caagaaaacg agaaataatt tcccctgatt
    17101 ttctcgtagc aaaaaaattc attggtaaag ttaacacatg tttgaaatac tgaagcataa
    17161 ttgccatact cattcgctgt cctaaaataa taagaagata actacgacta tattcaaaca
    17221 atacccgaaa taaatagaca gcgatcaagc caatggaaat tatatttaaa gttgaacgtg
    17281 cttgattagg gatgaaaaaa tcaagaatac cttgaaaata atatgagctt cctatgccaa
    17341 aaaacgtaat gaaaatcgat gctaaaacga tgtggaatac taatcctttt tgtttccaaa
    17401 ttataggcaa aaaagaactt aatccagcta ctttttcttt aataggaata taagtttctc
    17461 taggcatagg taataaaaga atacctgtcc attctttttg aaacgcttca atcgtttttc
    17521 tattttttcc attagcggga tctgcaatcc atagtacgtt tcccttcgtt ttataaatta
    17581 caacgtagtg catatagctg ttatcaacca atatatgtgc aattaatggt aagataagat
    17641 ctctttcttt ccagatctca ctatctgctt gaatagccat acaatcaaaa gacaattttt
    17701 caaatgtctt tttcaatcca aatgctgaag taccctctaa atcagtccca gataggtccc
    17761 gtaatttatg aatcgggata tcagttttaa aatattttaa aatcatcgaa aggcaagcaa
    17821 caccacaatc tttttcatct tgttgctgca caaaaggaaa tgattttatc acttatctaa
    17881 cacaactcct tctaaaaaat ttttatagaa aaattctcaa tatagcttta atacagtgaa
    17941 aatattgtct cataattcca caaaaacttt gaaaacagtt cttgaacact aactttttca
    18001 ttcctgaatc aaatttattt tggaaatttt actattcaag aataaacttg ataagtttca
    18061 ggaagatttt tactgttaat caaatacgta tgttacagta gcaccgtaaa aaatattatt
    18121 taaagaagcg tgtttaaaca tgcaaaatgt aaaggaacta agtattgtgg aaatgcacaa
    18181 actgttggtg gaggcaataa tgcttgggga aaattggggc aagttgtagg tggacttacc
    18241 acaggggcag taggtggtgc aggtttagga acagcaattt gcggaccagc ttgcggtgtt
    18301 gttggagggt tatatggagc tgtcgctgga ggtgctgcag ctggctggga tgctcgaaaa
    18361 aaataaaaaa ggagaataag tagtatgcat attaaaaaca caaaaacaac atttatatta
    18421 tcttcagaag agttgaaaaa tattcaaggt gggagtgccg tgggagtttt aggcactact
    18481 ttttctgggg caacagcagg agtaaaactt tgttcagccg gaggacctta tgcaatagct
    18541 gcttgtggtg taggtggagc tttattaggt gctggattca gcatgtggac cggtgcgtaa
    18601 acaattaaaa aatatcaaac ctcaagattg gtttgtgtcg attacgaaca taggtatgtt
    18661 ctttatcttt tcaaaagttt ggaatggaca gaacttttta ctaaagcttc ttgtgctagt
    18721 ttttataatt atattaaatt tttatctttt attaaagtca gataaaaatt ctttggaaat
    18781 gtaatggtag agatattttt acaaaaggac ttgcagcgct gtagttggtg ctgcaggagg
    18841 acctatttcg gccgttacag gaggtattgg taatgtactt gtcggatgtg cattaaagcc
    18901 tgttcctgca agttaaattt ataaaaacag gaaagtaatt atctcaaatc ctatcaagta
    18961 caatcatagg attgcacttg acaggacaaa ttttttaact aaaaaatgaa aaacactttc
    19021 taaaaattag tagaatttgc caaatctttc gcatcttcca ccatacattt aattttaatt
    19081 cccctttatg gtacttaata aaaacaagaa aagaactatt catttggccc ttttttggtt
    19141 ccagaccata gaaatattcc tccagtaact aatattgctg ttatgcacgt aactaataac
    19201 agctataaaa attggtacaa ctttaatagg attcagaaaa atttgcacta gtgaaggaat
    19261 ctattttatt agaaaaatgc aatctcccta aagaaaaaac cactaaacag tgctttcttt
    19321 caacttctcc ttaatttttt gtgctttccc taaagtaaag tttctggcca actctgaatc
    19381 aaaatatagt gaacgttttt taaaatctat ttctttaacg ttaaagatat ttactaaaca
    19441 cgctcgacta attcttgtta acataggata tttcttttca agctcactaa ttcttccata
    19501 aaattcgtac tgcccatctt tagtacacaa tgataatcta tgtggtaatt ttgaagcttc
    19561 tacaaaataa atgtcattta tatcaaaagt aaatgtttga gaacctatgg aaaaaacaaa
    19621 tgcttgattc gtaacctgtt ttgttgcatc tatcctttct tgcgctaata acaataactc
    19681 aacaatctcg tttcgatact catctagtgt ttgatctttt gttacaaatc ctaacgtttc
    19741 aactcttctt tttattgtta cgggtaacat ctcatcatga gtagttgtaa aaatgatttt
    19801 tgcttgaaca tcatattttc tgattacttc tgctaattct ataccgttaa cttcatgatt
    19861 taaatcaata tctaagaaat atattccttg ctttggtcgg aattgtttga ggtatttttt
    19921 aacttctaac ggactttgtg ttttcaatac tatcttaaat actttatcgt gaaaaagaat
    19981 gaaattatca ataattcgtt ctaactgatt taattgtata atttgatctt cacataatat
    20041 tattggataa ctcataaatc ctccttccct caaaacaaca aaataacttg agtagtaaat
    20101 tgatacggat cgttttgata gttcacaaac atattgggat tctttttatt gatttcctct
    20161 atactagata aacctaaccc ttgatgtccc ttttttgttg ttattccttt tttcattaga
    20221 gtatctatag agagtagtat ctctctacaa ctattcgata tcgaaatttc tatctgttta
    20281 tcatctcggt aaatagctag agacagttct ctatcttgac tctctataga tgcttcaatt
    20341 gcattatcga gaataattcc aagcacccta atacagtcaa atattggaac aggaacttca
    20401 ataatagtac ttggacattc gaatttacaa caaatcttat tgttatttgc ttggtatagc
    20461 ttcgctatca acaaactttt caaatattca ttttttatat tttttaaata acgatattca
    20521 aacttattgg ttagataaga attagaatag atctctaatt cttttatttc tttttccaat
    20581 atcgaatcat ggttttgatt agcgatttct tttaaactca gcaataaatt tttatagtca
    20641 tgtctaaatc tggcaaattt ttcttgatct ttttccagtg aatcagtata tttttttaga
    20701 taaaccaatt cttttctttt caattgctgt tcatattgtt ctcgttgcct aatagttatt
    20761 ctaataaata aaaatagaac aacaaacatt tgaatgatta aaaatattaa gacaccaaac
    20821 acaaaatgat caaatacttg atattgatga gcaacataag aaacaaataa accaattgta
    20881 agtaaataaa tcatgaatat agcagtaagt ttagaactgg actgttcaac aatcctattt
    20941 atagctaatt ttctatataa ccaaataaac atcagcaaaa acaaagaatc aagcattatc
    21001 tggaatacta cataaccata cccttttatc ccttcaaatg aaaacatata cgtcataacc
    21061 atggaagtaa taatagatct gaaatcatca ataagcatac ataatagtaa tttatttaaa
    21121 agcactatat tagttttctt cttttccttt aatataaaat aacttccgat tataggtata
    21181 gctgcggaaa catcagtaaa taattcaaca aaaaaagtga gtggtatcaa taaacataca
    21241 aaaagatatt ttcttttgtt aagatcagta ttagtcatat aaaataagag aaaaaagaaa
    21301 aagtttccca cgaacattct taatagataa gcttttgtat cgatagtaat caagagatcc
    21361 cctcctgatt ttaggatatc ataaaaaaaa ttttagttac actttttaaa taatgaaaaa
    21421 acacattcca ctaaatttga tgccggtttt ccttgaggtt ttgttccagc acctccttta
    21481 atttttttaa gctcttgatc tga
//
LOCUS       DAJSBM010000045        21375 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJSBM010000045 DAJSBM010000000
VERSION     DAJSBM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.045"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            56..757
                     /locus_tag="ONT67_001858"
     CDS             56..757
                     /locus_tag="ONT67_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR2355595.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="ONT67_001859"
     CDS             complement(1366..2382)
                     /gene="tsaD"
                     /locus_tag="ONT67_001859"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR2355596.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="ONT67_001860"
     CDS             complement(2379..2843)
                     /gene="rimI"
                     /locus_tag="ONT67_001860"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2355597.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="ONT67_001861"
     CDS             complement(2850..3392)
                     /gene="rimI"
                     /locus_tag="ONT67_001861"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR2355598.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="ONT67_001862"
     CDS             complement(3376..4098)
                     /gene="tsaB"
                     /locus_tag="ONT67_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR2355599.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4289..5269)
                     /locus_tag="ONT67_001863"
     CDS             complement(4289..5269)
                     /locus_tag="ONT67_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355600.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5293..6777)
                     /gene="galT"
                     /locus_tag="ONT67_001864"
     CDS             complement(5293..6777)
                     /gene="galT"
                     /locus_tag="ONT67_001864"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCR2355601.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6789..7778)
                     /gene="galE"
                     /locus_tag="ONT67_001865"
     CDS             complement(6789..7778)
                     /gene="galE"
                     /locus_tag="ONT67_001865"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR2355602.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8026..8193
                     /locus_tag="ONT67_001866"
     CDS             8026..8193
                     /locus_tag="ONT67_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCR2355603.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8255..10066)
                     /locus_tag="ONT67_001867"
     CDS             complement(8255..10066)
                     /locus_tag="ONT67_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR2355604.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10063..10428)
                     /locus_tag="ONT67_001868"
     CDS             complement(10063..10428)
                     /locus_tag="ONT67_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCR2355605.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10591..10986)
                     /locus_tag="ONT67_001869"
     CDS             complement(10591..10986)
                     /locus_tag="ONT67_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR2355606.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11004..11966)
                     /locus_tag="ONT67_001870"
     CDS             complement(11004..11966)
                     /locus_tag="ONT67_001870"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCR2355607.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11966..12178)
                     /locus_tag="ONT67_001871"
     CDS             complement(11966..12178)
                     /locus_tag="ONT67_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCR2355608.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12199..12897)
                     /locus_tag="ONT67_001872"
     CDS             complement(12199..12897)
                     /locus_tag="ONT67_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR2355609.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12917..13459)
                     /locus_tag="ONT67_001873"
     CDS             complement(12917..13459)
                     /locus_tag="ONT67_001873"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR2355610.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13591..14595)
                     /locus_tag="ONT67_001874"
     CDS             complement(13591..14595)
                     /locus_tag="ONT67_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2355611.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14592..15581)
                     /locus_tag="ONT67_001875"
     CDS             complement(14592..15581)
                     /locus_tag="ONT67_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR2355612.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15578..16384)
                     /locus_tag="ONT67_001876"
     CDS             complement(15578..16384)
                     /locus_tag="ONT67_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2355613.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16550..17506
                     /locus_tag="ONT67_001877"
     CDS             16550..17506
                     /locus_tag="ONT67_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2355614.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17583..18101)
                     /locus_tag="ONT67_001878"
     CDS             complement(17583..18101)
                     /locus_tag="ONT67_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2355615.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18189..18338)
                     /gene="rpmG"
                     /locus_tag="ONT67_001879"
     CDS             complement(18189..18338)
                     /gene="rpmG"
                     /locus_tag="ONT67_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR2355616.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18566..19012
                     /locus_tag="ONT67_001880"
     CDS             18566..19012
                     /locus_tag="ONT67_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCR2355617.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19207..21102)
                     /gene="asnB"
                     /locus_tag="ONT67_001881"
     CDS             complement(19207..21102)
                     /gene="asnB"
                     /locus_tag="ONT67_001881"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCR2355618.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6425 a         4545 c         3692 g         6713 t
ORIGIN      
        1 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
       61 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
      121 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
      181 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
      241 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
      301 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
      361 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
      421 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
      481 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
      541 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
      601 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
      661 agtaatcaaa caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt
      721 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
      781 cctgataaat atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
      841 gttttagatc actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
      901 cactgtttgc aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc
      961 tagattattc gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg
     1021 gataatgata aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa
     1081 aaaatagggc tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg
     1141 tcaactcttc ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg
     1201 ttccttgtga atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa
     1261 agcttgcata cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg
     1321 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac
     1381 caagcttgga tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg
     1441 tgctgctgct ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag
     1501 ttctacctca gggagtttag ctgccaaagc atcctgcaat ccttcacgca acccttgatt
     1561 ggccgctaca cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag
     1621 tgttttagta atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt
     1681 gtctagatct tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa
     1741 tccgctaaaa ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt
     1801 atcttttccc tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa
     1861 tactcttcca actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat
     1921 ttcatatgat ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag
     1981 tgccatcaat ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat
     2041 atggtttaca ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat
     2101 accaatcagc aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc
     2161 ttctgccgac acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac
     2221 gtgatggcgg ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg
     2281 agaagcaacg atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc
     2341 atcacaactt gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt
     2401 aactttctgc tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta
     2461 cctaatatac gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt
     2521 acttctaaaa aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa
     2581 tgccgtgcat gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact
     2641 tcatctatta ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc
     2701 agataatcag agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa
     2761 ccatgtggat aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca
     2821 aaatcttttt ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc
     2881 aacgcatctt cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc
     2941 aatttccgat aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc
     3001 tcacagcgat tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt
     3061 ttacgctgat atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct
     3121 cttgaaccga taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga
     3181 agcggtgact tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag
     3241 acttctcgtt cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg
     3301 taacgacgat cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag
     3361 cttctggcat acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc
     3421 caaccatttt tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc
     3481 tgcaactggt tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca
     3541 tgctggaact gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt
     3601 gtgacaatca ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa
     3661 atcagaagca gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg
     3721 gtatgcccca gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc
     3781 cacaccgatg caattagccg cgatggtttt cagactagat actcctacta gctctttatc
     3841 taatgtataa gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc
     3901 gggaccatca gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa
     3961 caattggtcg attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat
     4021 ttgtccaaga atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc
     4081 tattcctaat gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt
     4141 agcatacttt tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat
     4201 gattatctgc tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca
     4261 ctccattttc ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg
     4321 tttccacaat aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct
     4381 ccactgctag ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta
     4441 catatttcgc tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta
     4501 aacctttttc ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa
     4561 gcgcatcagg aacatgcgga ttcgttttta aatattgctt cactgcttga tatcctgact
     4621 cgacagaaaa atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt
     4681 gttgaaatgc cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg
     4741 ttccagcaag catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt
     4801 gaacacttga cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat
     4861 ctaccaaaag caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt
     4921 ttcctagtgc gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca
     4981 ataaagtaac gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat
     5041 aaagatcttc aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag
     5101 tatttgctgc atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt
     5161 tttgctttgt ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag
     5221 tagcaggaga aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct
     5281 ccttttttac gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc
     5341 tcgcttgaat actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg
     5401 tacgacatcg gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc
     5461 ccacggacga tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc
     5521 tactagttct ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc
     5581 aatgttctcc tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg
     5641 ttcttctgaa acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct
     5701 tcgtgcaatt ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga
     5761 tactgtttca tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag
     5821 caagtttttc tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc
     5881 gatgacttgc ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg
     5941 aaattcatga cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg
     6001 taagtcagca ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct
     6061 agcaaatgtt tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg
     6121 ttcattgtaa taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat
     6181 tcgaataatc cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt
     6241 ttccatgcaa agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat
     6301 ttgttttgga tctttttctg gcttagataa attgattgtg atttccagtt caccatactc
     6361 cgactctgcc ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt
     6421 cttacttaaa ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc
     6481 aaagaatgca ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa
     6541 aatctcttgt tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag
     6601 ctgatccaat aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc
     6661 atcttctcct accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca
     6721 tccgccagct tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc
     6781 tcattcccct attcttcata accatttgga tggctgacat gccaatccca tgctgttttg
     6841 ataatcgctt tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca
     6901 cttgaagcga ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt
     6961 tcttttcctg tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg
     7021 ctgcctaaat tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata
     7081 tgtgcagcaa tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta
     7141 tcataatcgt ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg
     7201 attgggacaa gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct
     7261 ccggctacat tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac
     7321 ttcatcattt tttccatcat caatttactt tcaccatacg gatttttagg attagctggt
     7381 gtatcttctg tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat
     7441 acgattttat tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg
     7501 ttgttgttga aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca
     7561 aaatggatta ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc
     7621 cggatgtctc cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc
     7681 aaattatcta ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa
     7741 ccgatataac cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt
     7801 aaaattgtgt ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa
     7861 caaacaaagt aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac
     7921 attttcaagg gttttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct
     7981 tttgttccat aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta
     8041 tctggaatat tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggttgcttct
     8101 gtgaaaaaaa cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt
     8161 aaaaaagcaa tgcgtaaacg cgtttctcgt taataattat ttaaaaatac gcatgaagac
     8221 tttctaaaaa acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg
     8281 ctattttatc tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag
     8341 ttagccaatc cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc
     8401 ctgaagcatc aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac
     8461 ttcctgtatt atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta
     8521 tgacaggatt acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc
     8581 gactgctgat ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat
     8641 caaatggcgg ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg
     8701 tccgaatgat tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata
     8761 gtatttcttt gtattcctta gcagaaaaaa cgtgccattc aatagcctgc cggattggat
     8821 cacgccagtc ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt
     8881 ccgcttcttt tatctgccct tcaataaata ttcggctagc ttctgaaaag ggctcattct
     8941 gataggcaag aggagtttct aaatacgacc aatcccatct ataagggatt agtgctggat
     9001 gctttccgag acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg
     9061 gaatcggcaa gtgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt
     9121 tcagatagtc aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt
     9181 tttcttttgt tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga tttcttcctc
     9241 gagggtaata agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg
     9301 gatgatagac cagttttccc ttttcgttct cgaagcgccc tcctcttgat actgtaaaca
     9361 atccgacata atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg
     9421 gtatcttcga aagatctaca tctgcaggat tagaaggaag ctgatacagt aaaacatttt
     9481 tctttgcgta gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc
     9541 ttgctaaaac aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat
     9601 cttttctttt gcttactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt
     9661 cttcttgcaa ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt
     9721 gctgatcttt ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg
     9781 tctgttcgat ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg
     9841 ttaccaatcc tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag
     9901 aattcattaa taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg
     9961 gatcgaaaag aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg
    10021 ctacacttaa tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat
    10081 ttcgataaat tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac
    10141 aaaagtaatg actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt
    10201 ttcttcttcg aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg
    10261 tacatggtca aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc
    10321 taactgcgct ttttcttttg aaattcctcg tggccagaat ctatccacga gtattctata
    10381 gccatcctct ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt
    10441 ctttctacct tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa
    10501 cggcacaact atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg
    10561 gtttttaaag ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga
    10621 ccatccgtca tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt
    10681 atttgctgta cggatgctca aacttttctt ttgatcgtaa atatagacag gctggtcttt
    10741 tcgaatagat cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat
    10801 atgcccagca tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc
    10861 ttctcgaaat tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata
    10921 gacttcccaa aggatgattg caagcacaat caatagcaaa atgatattaa ttacccataa
    10981 aaccatgtaa tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg
    11041 acaccagctt cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg
    11101 aaagtaaatt ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat
    11161 actcctccac ctaacacgat acttgacgga tttaatgtat tccccaagtt tccgcaagct
    11221 aaacctaagt agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga
    11281 gccatttcaa agatatcttt acttgttact tcttctccgt tgtctagcat tgctttcagt
    11341 tttgaatccc ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac acctgtagcg
    11401 ctagatacag tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc
    11461 acagtgatat gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa atttccgcca
    11521 gcaacgatac ctccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt
    11581 tctccagcac ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg
    11641 aatttgattc ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt
    11701 aagttatacg caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg
    11761 ccgattccga taaagtcttc ggctttcata ttatataaat tcaaacgatg attgattgat
    11821 tcaatgattt caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat
    11881 ttttgctgaa tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa
    11941 tcaatcccaa taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc
    12001 ggtgctctct ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc
    12061 tatcatacag attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca
    12121 catagataaa gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct
    12181 ttaagcactt ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca
    12241 tattacaaaa agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct
    12301 tgtctgttgt ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc
    12361 gacatgtccc caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat
    12421 aaataaaaga aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc
    12481 taagataaac aacgcaccga acaaaccgaa tatcgaagta cttgcaccac cagacagaac
    12541 attcgcttcg ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat
    12601 caagaaaaaa cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat
    12661 cagtgagttc attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg
    12721 ccaccattca ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc
    12781 aagtcgcgga aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata
    12841 ggtccataac ggtcgtctta accatgcacg caattttatt tgctgttgat aattcatgaa
    12901 aacctcccat ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg
    12961 gttgccattg ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat
    13021 tatttaaata ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt
    13081 agataatacc tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt
    13141 gtggctcaag aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa
    13201 acgccatctg acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg
    13261 ctcgttggat aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca
    13321 cttttgctga cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt
    13381 cctttttctt gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc
    13441 ttactttcaa ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa
    13501 aagaaaaata gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag
    13561 aaatgattgg ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat
    13621 aaaaagtgca tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac
    13681 ttccccactt ggtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc
    13741 gaacaaaccg ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc
    13801 atgaggagct agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc
    13861 caaagcaaca gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc
    13921 ttgcgcgcgt tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa gtcctcctag
    13981 cggaattaag ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca
    14041 gattgatccc gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt
    14101 gcatagcaat gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg
    14161 attcataccc aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa
    14221 aacaccacct agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa
    14281 acctaaataa agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc
    14341 tgccaattca tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc
    14401 tgctaataaa ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga
    14461 agatgattct ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc
    14521 aatcattaaa ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca
    14581 atatattttt ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc
    14641 aatgcaatga ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa
    14701 tccgccaaca taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag
    14761 tccgttccaa ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt
    14821 ccgactaacg aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc
    14881 gttcgtttag gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat
    14941 attgcttttc ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact
    15001 gctaattgtt tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc
    15061 tgcagattaa agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct
    15121 acgccagcta aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca
    15181 aatacgagca aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca
    15241 gaagctttag gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt
    15301 cctagtaagc ctgaatctgc taaaggattt cttgtcaccc cttgcatcaa tgcaccgctc
    15361 aatgcaaaac aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt
    15421 atgatttggt gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct
    15481 ttttccacag tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa
    15541 cagaccaaaa gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt
    15601 ttggcaacaa atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg
    15661 ttgcatcaat ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac
    15721 cttctgtgat caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat
    15781 gattaatgtc atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca
    15841 ataactgtag gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga
    15901 tcaagatatc tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac
    15961 cagacagcgt tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc
    16021 actcaattgc ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc
    16081 gtccgtaaga tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta
    16141 ataatgcaat tttttttgca agttcttttg aactgtatgt agagatatca gagttattga
    16201 caatgatctt tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt
    16261 ttccacttcc atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca
    16321 tgttttcaat gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg
    16381 tcatttcacc catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc
    16441 gtcaagcgct actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca
    16501 ggaataataa ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa
    16561 aacaaaagta ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc
    16621 cccctcttca gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac
    16681 tcatacagtc acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat
    16741 catcggcagt tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg
    16801 gactgttggc agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac
    16861 gatttcttat gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat
    16921 ttcttcttcc gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac
    16981 ttatgttgta aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa
    17041 agctttggca aaagaaaaag aggcagaaaa aatcatttcc gattatcaaa aatccatcaa
    17101 gaaaactcga gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt
    17161 gaccaacaac tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga
    17221 cgatctggaa tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga
    17281 ctggtctgct gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt
    17341 gaacagtgac aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc taaaagcagt
    17401 gacagacaat catgtactgc aattcggacc tgaatctagt tggctatata acggacctat
    17461 tgccagtcag gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa
    17521 agcatcttca cgattagtcg tgagatgctt tttttcttat taacaaacta gacgcataga
    17581 ttttattctt cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg
    17641 gcatatagat tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa
    17701 aaatcaatat tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag
    17761 atccgaccag tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata
    17821 tcttctgaga tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca
    17881 ttttctttcg tgcgaaaatt tggttgcgtt gtcaacacga tgatatggcg ataaggaagt
    17941 gaaagatccc ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta
    18001 tgttcgcgaa agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg
    18061 atcagaccat ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat
    18121 tttagatgtt ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt
    18181 taaaaacatt attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt
    18241 tgtttttcca aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct
    18301 ttacaagaag tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca
    18361 agaatcctct aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct
    18421 agtgtttttc acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt
    18481 ctcaaacaaa atctttttct ttttgtataa atacccttac acttgtttat atagattatg
    18541 ttttttaata aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat
    18601 gaaaaactta gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga
    18661 tgaagggtac cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta
    18721 tgaagatgct agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac
    18781 ggatgaacac gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga
    18841 atatgaggaa gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt
    18901 tgatacaaaa aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac
    18961 tttgttcatt tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt
    19021 aaaggggttg tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct
    19081 ctgccaactc cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa
    19141 ttgtttctta tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga
    19201 ttttcatcaa acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa
    19261 gtagacttta taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt
    19321 acgtccttcg tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt
    19381 tgcaaaatct gcttcgaata atgctttgac ttgttcgtag ccgtgttctt ctttcaacca
    19441 agctttgatt ggcacaggga atcctaattt cacacgattc gaccattctt ccggaagatg
    19501 tttgttcgca gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact
    19561 tggtatttgt tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa
    19621 agaacttgcc atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg
    19681 aagatcgaca tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg
    19741 aacagtcatg atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga
    19801 tgcttctgat tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag
    19861 tgattcatat aaatggatcc gtccatggaa gtttttcatt tttccgattt tacgtcctaa
    19921 attatagcgt gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt
    19981 tgaattcgta tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc
    20041 agattggact acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca
    20101 agaaggattt gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga
    20161 catgtctccg tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct
    20221 ggcttcaatc gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg
    20281 tttcaataca gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc
    20341 tacatcactg accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat
    20401 ccattcttgt cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat
    20461 gtctattttt ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt
    20521 gaagaatgtt tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc
    20581 tttgttcaat tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa
    20641 cataaatgtt ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc
    20701 accgaataac tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt
    20761 ttgcaataat tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg
    20821 tgtttgaaaa acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat
    20881 ttcgccatta aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc
    20941 ctctaagtca ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc
    21001 tcctgaacta ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt
    21061 tttgttgtcc ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa
    21121 tataaaatta ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa
    21181 tattttctct cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca
    21241 gaaaataaag tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc
    21301 ttcgtgttaa aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg
    21361 acctaaagat cgtgt
//
LOCUS       DAJSBM010000046        21236 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJSBM010000046 DAJSBM010000000
VERSION     DAJSBM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.046"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(44..691)
                     /locus_tag="ONT67_001882"
     CDS             complement(44..691)
                     /locus_tag="ONT67_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355619.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(870..1589)
                     /locus_tag="ONT67_001883"
     CDS             complement(870..1589)
                     /locus_tag="ONT67_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355620.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(1720..2871)
                     /locus_tag="ONT67_001884"
     CDS             complement(1720..2871)
                     /locus_tag="ONT67_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCR2355621.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(3088..3828)
                     /locus_tag="ONT67_001885"
     CDS             complement(3088..3828)
                     /locus_tag="ONT67_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2355622.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(3832..4155)
                     /gene="rsfS"
                     /locus_tag="ONT67_001886"
     CDS             complement(3832..4155)
                     /gene="rsfS"
                     /locus_tag="ONT67_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR2355623.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(4212..4805)
                     /gene="yqeK"
                     /locus_tag="ONT67_001887"
     CDS             complement(4212..4805)
                     /gene="yqeK"
                     /locus_tag="ONT67_001887"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCR2355624.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(4789..5439)
                     /locus_tag="ONT67_001888"
     CDS             complement(4789..5439)
                     /locus_tag="ONT67_001888"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR2355625.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(5449..5760)
                     /gene="yhbY"
                     /locus_tag="ONT67_001889"
     CDS             complement(5449..5760)
                     /gene="yhbY"
                     /locus_tag="ONT67_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR2355626.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(5776..6885)
                     /gene="yqeH"
                     /locus_tag="ONT67_001890"
     CDS             complement(5776..6885)
                     /gene="yqeH"
                     /locus_tag="ONT67_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCR2355627.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(6882..7409)
                     /locus_tag="ONT67_001891"
     CDS             complement(6882..7409)
                     /locus_tag="ONT67_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCR2355628.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(7589..8374)
                     /locus_tag="ONT67_001892"
     CDS             complement(7589..8374)
                     /locus_tag="ONT67_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR2355629.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(8367..9236)
                     /locus_tag="ONT67_001893"
     CDS             complement(8367..9236)
                     /locus_tag="ONT67_001893"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCR2355630.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(9237..10616)
                     /locus_tag="ONT67_001894"
     CDS             complement(9237..10616)
                     /locus_tag="ONT67_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR2355631.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(10624..11043)
                     /gene="fabZ"
                     /locus_tag="ONT67_001895"
     CDS             complement(10624..11043)
                     /gene="fabZ"
                     /locus_tag="ONT67_001895"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR2355632.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(11068..11544)
                     /gene="accB"
                     /locus_tag="ONT67_001896"
     CDS             complement(11068..11544)
                     /gene="accB"
                     /locus_tag="ONT67_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR2355633.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(11547..12782)
                     /gene="fabF"
                     /locus_tag="ONT67_001897"
     CDS             complement(11547..12782)
                     /gene="fabF"
                     /locus_tag="ONT67_001897"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR2355634.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(12801..13538)
                     /gene="fabG"
                     /locus_tag="ONT67_001898"
     CDS             complement(12801..13538)
                     /gene="fabG"
                     /locus_tag="ONT67_001898"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCR2355635.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(13550..14467)
                     /gene="fabD"
                     /locus_tag="ONT67_001899"
     CDS             complement(13550..14467)
                     /gene="fabD"
                     /locus_tag="ONT67_001899"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR2355636.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(14574..14798)
                     /locus_tag="ONT67_001900"
     CDS             complement(14574..14798)
                     /locus_tag="ONT67_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR2355637.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(14851..15813)
                     /locus_tag="ONT67_001901"
     CDS             complement(14851..15813)
                     /locus_tag="ONT67_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCR2355638.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(15823..16263)
                     /locus_tag="ONT67_001902"
     CDS             complement(15823..16263)
                     /locus_tag="ONT67_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2355639.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            16569..17534
                     /locus_tag="ONT67_001903"
     CDS             16569..17534
                     /locus_tag="ONT67_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCR2355640.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(17591..17821)
                     /locus_tag="ONT67_001904"
     CDS             complement(17591..17821)
                     /locus_tag="ONT67_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCR2355641.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(17907..18794)
                     /locus_tag="ONT67_001905"
     CDS             complement(17907..18794)
                     /locus_tag="ONT67_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR2355642.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(18883..20106)
                     /locus_tag="ONT67_001906"
     CDS             complement(18883..20106)
                     /locus_tag="ONT67_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2355643.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(20123..21169)
                     /locus_tag="ONT67_001907"
     CDS             complement(20123..21169)
                     /locus_tag="ONT67_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2355644.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         5757 a         4708 c         3594 g         7177 t
ORIGIN      
        1 caataataat tgcagaaaaa gtaattcaat catttaaggt catttattct atatataaat
       61 ccaaatctgt ttcaggatat tttaatttga caagcgaaat aatcattttc agttcttcca
      121 tgttttctgg atattctggg tcaaatacct tgctatgacg gccaatcccg ccattgtcga
      181 tcaatcggta aattcgattc cattcaatga attggtcaat tgattggaaa ttgctgattg
      241 gatagccgcc acaatccaaa atctctgata cacgaaatgc taggtatgca ctatcgatca
      301 ctttattttt tttcgtgtct atcttctcat ttctttcgat aagaattttt tcaggacgat
      361 gtttcaagct ccactctaat agtagaaaga aagcctccat ccgatactct tcttcaaata
      421 accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat cagataattt tgtctataaa
      481 tcagcattgg tgcgcggtcc accatacact ctaaataacg gttcagatac cctgtaccga
      541 catagtttcc taatcgatag ccttcaacgc cgaatcttgc gtatttttgc gctaattttg
      601 aacaatcagt tacgttcaca gctaactctt ctactttgcg gtaaccgctt aagatacgtt
      661 caaaaggttc taaagtgatt tttgcttcca tattcttccc cgtccatctt ccacatactt
      721 catatcattt cacatgatac ttatgctctc atcataccac aggttgaatt agaaaagaaa
      781 ataaatcaca caaaaaagca agacaatctg ttagtgagat tgtcttgctt ttcgaaaaag
      841 actgaatact tttcgttaaa actggttgct tagtcttcta atgccacatt atggaacaca
      901 tgctgtacat cttctagatc ttccagtgca tcaatcattt tttcaaatgt ttcttgatct
      961 tctggagata gtgttacctc gttttgaggg atcatttgga tctcagcgat tgaaaaatca
     1021 gtgattcctt tttcttttaa tgcttcttgt atactatgga aatccgctac ttcaccgtaa
     1081 acgatgattt gtccttcttc ttccattaca tcgcgaacat cgatgtcttt ttccattagg
     1141 tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa agattgctgt attgtcaaac
     1201 atgtaagaaa ccgctccgga aacacccatg ttcccgccat ttttcccaaa tgctgcccgt
     1261 acgtctgctg ctgttcgatt cacattgttc gtcaatgtat caacaatcac cattgagccg
     1321 cttggtccaa atccttcata gcgcaattca gagtatgttt catctcctgc accttttgct
     1381 ttttcgattg ctcggtcaat aatatgtttt ggtacattat aagttttagc gcgatcaatg
     1441 acgaaacgta gtttttggtt tgcatgcgga tcaggatcac ctgattttgc tgccgcgtag
     1501 atttcaatcc caaattttgc ataaatccgg ctattattag catctttagc agttttcttt
     1561 gctacgatat ttgcccattt acgtcccata ttttcacttc gctttctgat ttgatagttt
     1621 cataacgcaa aaacgtcctg ttccattata cttgtgaatc acgattctgt gaagcttttt
     1681 ttctttcttc cttcattctt ctcattttta gtcaattatc tatactcgga ttgggatttg
     1741 cccgaaattc tgttccggaa tattgcctcc caattgatag atttgatcac ttctaactaa
     1801 cagttccgct tcttgtgcct ctttttttcc gattcgggaa taaatagaaa tctctttttt
     1861 ttcttttaag aatcttcttc cagcatcggt aaatccaagt agtcgaagat aatttttatt
     1921 ttgttggaca ggaatctctt gctttttgat attcagcaag acataacaag ataatcgttg
     1981 gatccgtgtc catgtatatc tttttgtttt tacctttgac acaaaatcct caaaactatc
     2041 agcagttgca gctgctgttt tcatgcgcga ttctattcct tcggtcatct gatagatatc
     2101 tgtcagttcc agcaaagtag aagataacaa ccgataacgc aaatacggcc aataagattc
     2161 ccaagtctgc agcgaacaag ttgaaagatc ttcttctgtc tgaggcggca caagtccagc
     2221 tattcttttt ccttcttcta acgcatggcg aatggctgtt gcactggcta tcctctgatc
     2281 tgtgatgatt ttttcatgat agcctgcact tttacgtcgg atcggatgta attccatagg
     2341 gtggacatac ttggcatttt cctttgcata acttaatccc aagatatgat ttggcgaaga
     2401 aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc tcagtcattt tttgcggata
     2461 actatacgtt tgatcagaca gttcttgaaa tgcctcttct atcctatctt gattctcttg
     2521 gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct tcttctgttc caaaacacaa
     2581 actgtcacag tccagtgttt gcaagatttt tactgcacct ttagcaaaat agtcagctga
     2641 ctgaaccgcc cattcgacgg gaagctccac tactaaatcc acaccatttt gtaaagcttc
     2701 attcgcgcga tgccatttat cgatgatagc cggttcgcct ctttgcaaaa aattcccgct
     2761 cattacagca atgacgacct ctgctcctgt tatttttcgt gccatttcca catgatagcg
     2821 atgtccattg tgaaaaggat tgtactctac gataatcccg cagcttttca tgcgcatcct
     2881 ccttcatagt tatcgataaa aaagaaatag agacaagtaa aaaaaggcaa aaatcggaaa
     2941 aaatacccga actatataaa ctattctcgc aaacacaata cttctgcgac gaaaagctgc
     3001 actgcaagag caatgcctat atcgttcgga tatataattt atgttgaaga tttattcttt
     3061 tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat gctacaaaga accagcgctt
     3121 tgatttctct gatgctgctt cgtctgtaaa atcagaaaat gcttcgactt tcgaaaagcc
     3181 gctgttttcc agcattctta aataactttc caatggatag gtacgctctt gatgtagttc
     3241 atcttcacga ataaatcgac cagacccttc ttgttcttca acgaaaaagg taagaaaatg
     3301 ttcgatgctg tgtttctcgc ttcctggata actatcccac aaaaaagcaa aattatcccc
     3361 ttgataatga tagctatatt ctggaaaaac ctcatctact tgataggtgg agtgtacgtc
     3421 aaataaaaat cggccatttt cttctaacgc ttgatagact tcatcaaaca cttgctgtac
     3481 ctcttgtcta tttttcatat agcacagcga atcagaaaaa caggtaattg cctgatattg
     3541 tccgatttct gataaatcga gcatattgcc ttccacaaat tggatcgatg cgtcttcttc
     3601 atatgctcgc tggctggcaa tcatcaacat ctcttctgaa aggtctaacc ctgtcacttt
     3661 atatccatct tttgcaaagg caaccgctaa tgctccagta ccgcatgcca gttccaaaat
     3721 attttttgtg tcttcttgta aatgacgttt cgaaaaagct aaccattgct gataaagtgt
     3781 ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca taagccatat gttaatccaa
     3841 ccattcacta agatcgacta gcggagcatc tgaccatagt ttttccagat tatagaaatt
     3901 tcgttctggc gcatggaaaa catgtacgat cacatctcca agatcgatca atacccattt
     3961 tccgccttct tttccttcaa tacgttttac ttcatatttg ttttcttctt ctttatcaat
     4021 gatttcttct gtaattgcgt tgatttgacg ttcgctattt gctgaacaga tcatgaaata
     4081 atctgccaac aaggaaacat ttcgaacgtc taaagcgata atgtcttctg cccgttttga
     4141 atctgctgct tcaatcgcaa tttttaattg ttctttacta tctatgatga tttcctccta
     4201 atatgagcag cttatttttt tgctacccag taattatatg tttcgattgt ttttggataa
     4261 atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt ttgtttcaaa tgctacagct
     4321 tcgtctagat cagcccatgc aatggctcgt gcatcttgta ccccaggaaa atctcgacct
     4381 ggttcaatat agtctgctac ataaatgatt ttgtcaagga gactcatctt agcagcccca
     4441 gtcgtatgca cccggattgc atgaagaatc tctgcatcgg tgattcctaa ttcccgctct
     4501 acaaacgaag cgccaacaag accatgccag attgcattat tataattcaa taactccgga
     4561 tcaaaaccgt ctcttacgat taccattttg aactcctcat ctggtcgttc ttttgcataa
     4621 tcatgtgtca atgcagcaat actagctttt tcaggagaag caccgtattt tttggcaaga
     4681 gccacggctg tttcttctac tccaagcaca tgcttgaagc gttgttcgct cattctcatc
     4741 tgtatttttt gcatcaggtt ttctctagaa taagcagtat attcattact atagtccata
     4801 ttcatacaac ccttcatttt gaatataatc aattacttta tctggaacaa ggtagcggat
     4861 agaacagcct tctttgatct tttgacgtat tttcgtggaa ctgatgtcga tctcaggtac
     4921 atctacccaa attacaggat atggagtatc cgttgtgtat cctggtcgac gtatacccac
     4981 aaagttgacc atagaagtca gctcatcgat tttataccat tttggcaaat attctaccat
     5041 atccccaccg ataataaagt aataatctgt atccggattg ttttgcgtca attccttcat
     5101 agtatcatag gtgtaacttt tcccaccacg agctaattca atcgtttcaa tctgcaaaaa
     5161 aggattgtct tccaccgcta attccaacat gttgagtcga tgttttgcat caatcgtctg
     5221 tttctcatca acatgaggtg gctgataaga aggcatcaaa aatacgcggt caagacctaa
     5281 attccggcca gcttgttcag ccatcactaa atgtgcaagg tgaacgggat tgaatgttcc
     5341 gcccaagatc cctacttgct ttttcttctc taagaattga ggcatttctt caggaaataa
     5401 ttgtgaacga acaaatgtct tagcttgcgt attcatcatg gtcactcctt atattgcttt
     5461 tacttctttt gatagccttt gatacttttc ttttgtggaa ggtttgtata atacaataac
     5521 acgtccaatg atttggacga tttcgcaatc tatttctttt tctaatactt gagctgcttc
     5581 ttctgcaatc tcatctgtat tttgaagtaa cgatactttg atcagttctc ttttttctaa
     5641 agcttcttca atttgaacga tcattgctga attgatccca ccttttccga tttgaaaaat
     5701 cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt tgttttcctc ttaaactcat
     5761 aattttcctt cttccttaaa tcaatgcttt gcgtttcaat acatctacac cctttggcgc
     5821 ccagcccgca attactactg gcgctgttac agtgatccag cctagaccgg caaaaacgat
     5881 gtctgttttt tctttcacag aaaactcaaa acgaacaagc tccggaaatg cctcgacttc
     5941 atcaggacga ggaggctgta ataatccacc tacatgtttt tgataaaatt cgtctgcttt
     6001 ttccagtttc gttcgatgaa tatttacatc atttgacaca taagcaataa atgaagcacg
     6061 attgccgctg ataaaatcaa atcttgcaag tccgcccaag aaaagtgttt gcccttcatt
     6121 cagctgataa actttaggtt tgatttcttt taacggtgca atgatccgca aatctttttt
     6181 acctaagtag tgcgccattt gatggcgatg gataattccc ggtgtatcaa tcaaaaaatg
     6241 cccgtcatct aaaggaatct cgattttatc caaggtcgtt cctggaaact gcgaagttgt
     6301 gattacatct tgtactcccg ctgtattttt gatgatttga ttgataagcg tcgattttcc
     6361 gacatttgtt actccgacga tatagacatc ccgtccatcg cggtattctt cgattttttc
     6421 taatagttcc gtcatctcat gagatttctt agcactagtt aataatacat ctactggacg
     6481 aattcctgct tcgtaagcac gttccttcat ccattgtgtt aatttagttt ttttcaatga
     6541 tttaggtaat atatccactt tgttcccaac taaaagtact ggattgtcac cgacaaaacg
     6601 gtgtagtcct gggattagtg aaccattgaa gtcaaaaata tccaccacat tgacgatcaa
     6661 tgcatctttt tgccctaatt cattcagtaa gcgcaaaaag tcgtcatccg tcaatgagac
     6721 atcttgggtc tcattataat ggcgtaatct gaaacaacgc tgacaataga cttccccgga
     6781 agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct aattcaccgg gtttgtttgt
     6841 ttgtataacg gcaccacagc cgatacattg taattcttct gtcattcgat tccacctcgc
     6901 cagcccatat cagggtgttt tgctaataga taggccataa ttcttttttc ccaaaaacga
     6961 ttaaattgtg tcttccaact atctgttgcg ataatgggtt ttacaaggat gctacgtatc
     7021 cctgcacgat tggctccacg tatatcagtc ataatctgat cgcctaccat tactatctca
     7081 tcatctgcta aatccaattg tttctttgct atgttgatcc ctcttgctaa aggtttcatt
     7141 gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc gcgcgacacg ttttgaatta
     7201 ttgtttgaaa caacaacaac agggattcct gcatttttca tttctaaaat ccaagtaagc
     7261 aactcttctg ttccatctgg attattccat gcaatcaacg tattgtctaa atcggtcaat
     7321 actgctttga tgcctagatt ctttaattgg gcaggtgtaa tctgatagat ggcttctacc
     7381 atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc atctgcaaaa aatgaaggag
     7441 cacataaagt actcctcaac cccgtattat actgcatatc agcaaaaaaa actacctcta
     7501 tactcgttcg tctttcagaa aaagcatttc tttacaaaat gaccaaaaat aaagattgat
     7561 cacagaaaaa tttagaaaca aaaaacaact aatattggcg gaacctcttg tatcgttttt
     7621 ccagtaattt atgctgatct aatcgaacaa gttccgtcaa tctttcaatc aacgcttttt
     7681 tcataatgtg tgcgacttta tatcgtggca actgctcgcc gttgaacgtt tcaggaataa
     7741 ctcgatcaac gattcctaac tcgcgtagtt ctggcgcagt aattttcatc aactcagctg
     7801 cttctgcggc gcgactaccg tctttccaca aaatcgacgc aaatccttct ggagaaagca
     7861 cagcataaat cgtttcttcc agcatccaga cttcatccgc taaacctaga gcaagggcac
     7921 cgccacttcc accttcacca atgatgatgg cgatgatcgg tacctttaaa tcagacattt
     7981 cgagcagatt tctggcaata gcctctcctt cgccgcgctc ttctgctcct acaccgcaat
     8041 atgcacctgc tgtattgatc aaggtaatca ccggtctatt gaacttttcc gcttgtttca
     8101 tcagtcgtaa agcttttcga tagccttcag gatggggaga accaaaattt ctgcggatat
     8161 tttcctgcag atcccgtcct ttttgaatac cgatcactgt taccggtttg ccatctaatc
     8221 gagcgattcc accaacaatc gcttcgtcat ctgcaaataa acgatcacca tggcattcct
     8281 cgaaagaatc gaagatctct tcaaaaaaat ccaagcttgt ccatcgttct ttagctcttg
     8341 ctaactgaac gacatcatgt gctgtcttat ccacgatcta tccatccttt ctgtgtatgc
     8401 aattcaagaa gcgtatggat tttctgtttc aattccatgc ggggaacaat ctgatcgaca
     8461 aaaccatgag ataataagaa ttcagccttt tggaaatctt ctggaagttc ctgcttgatc
     8521 gtctgttcga tgacgcgtct tccagcaaaa ccgatcaagc tttggggttc tgctagaata
     8581 atatcgcctt ccattgcaaa actggcagtt actcctccgg tcgtcgggtc cgttaaaaca
     8641 gttagataaa aaagaccttc attgctatgc cgtttgacag cagcagagat ttttgccatc
     8701 tgcattagag aaaagattcc ttcttgcatc cgtgcgccac ctgaagctgt gaacaagacg
     8761 actggaagac tttcctttgt tgctcgttcg aataatctgg tgatcttttc tccgacaatc
     8821 gttcccatac ttcccatgat aaaattagga tccatcactc caataatgaa gggaatcccc
     8881 tgaatggttg ctttaccagt gaggactgct tcatgaagtc ctgttttatc ttgcattttt
     8941 ttaatttttt ccgtatattc gggaaatgct agcggatctt tggttacaag atccgtatcc
     9001 cattcttcaa aacttttttc atcaataatc aaagctagac gctgccatgc accgattcga
     9061 aaagcgtatc cgcaatgagg gcatactttt tcttcaccga tgtctttcgt ataaaggata
     9121 tgtttgcaat ttggacactt tgcccacatg ttatcaggaa cagatggcga gttagcaggt
     9181 ttgctctctc tattaggatt gatccgaata tatttctttt tcttaaacag agccatctat
     9241 gcgcctccta tctccgtttc tggtgtccaa ttcggtaaaa attcttcttg taaaaatgct
     9301 gtgctgtaat cacctgctat gactgcagga tgactaatca aatccatctg aaattctgcg
     9361 ttcgtgatga ttccttctgt gataaattca cttaaggctc gctgcatctt cattaatgct
     9421 tcaaaacgcg tatttccatg gacgattatt tttgcaatca ttgaatcata atatggcggg
     9481 atcgtatatc ccgggtaaac tgcactgtct actcgcagtc ccatcccacc tgctggaagc
     9541 agtaagtttt ggatcttccc tggagaagga gcaaaatgga aagcaggatt ttctgcatta
     9601 atccggcatt cgattgcgtg tccttggaat actacttctt cttgacacac attcaaaggc
     9661 tcaccggctg ctatctccac ctgttttttt actaaatcga ttcctgtgac catttctgtt
     9721 actggatgtt ccacttggat tcgagtattc atttccatga aataaaaatc gccttcttca
     9781 tccatcagaa actcgatcgt tccggcattt tcataattga ctgctttagc tgctcgaaca
     9841 gcagcgtctc ctagtgcttc tctttttgtt tgtgagatca ctacagaagg agattcttcc
     9901 agtacttttt gattgttgcg ttgtaaagaa caatctcgct cgcctaaatg aatgacatga
     9961 ccaaatcgat cacctaagat ctgaacttct atatggcgag caggataaat gattttttcc
    10021 aaatacatat catcatttcc aaatgccgct gctgcttctt gctgagctga agaaaaatgc
    10081 tgtgggagct cttcttttga ttgaactttc cgaatgcctt tcccgccacc acctgcagct
    10141 gctttcagca tcactggata accaattctg tctgctattt ccaatgcttc ttctactgta
    10201 gataatacac cagtacttcc tggaatgact gggacatttg cttcttgcat caattgacgc
    10261 gcattgatct tatttcccat tgcatcgatc gttgcacttt taggaccaat aaatgtgata
    10321 ttgcactctt cacacatgga ggcaaatcgg ctattttcag ataaaaaacc aaatcctgga
    10381 tgaattgctt cggcttttgt cacgattgca gcgctcagta cttcctgaac attcagatat
    10441 gaatcaacgg cttttgccgg gccaatgcag attgcttcat ctgctaactg tgtatgaagt
    10501 gcttcggcat ctgcttctga atatatcgct acggtttgga tccctagttc gcggcatgca
    10561 cgaatgatac gtacagcgat ttcaccgcgg ttagctatca atactttaga aaacatattt
    10621 cacctatcca atcataaagg ttaattccgc ttcggctact tttttaccgt ctactttagc
    10681 gattcctttg cctattccgg ctgaagcttt cactttcaat atctcaactt caaggtataa
    10741 ggtatctccc ggtgtaactt tcttacggaa ttttgctttg tccaatccgc cgaaataagc
    10801 cgtctttccc ttgaattgtt caagagaaag taatgcaact gctcctgctt gagccattgc
    10861 ttcaacaatc aatacccctg gcataacagg ttcttctgga aaatgtcctt gaaaaaaggg
    10921 ctcattgatc gttacatttt tcttagcaac gatccgttct ccttcaacca tttcttccac
    10981 tcgatcaatc aaaagcattg gataacgatg tggaatgatt tcttttattt cttgtatgtt
    11041 cattgttttc cctctttcct gctttgctta tttttgctga atcctaaaga gtggctgtcc
    11101 atattcgaca actgactcgt tttctacaag gatttctgtg atcactccgt cggcctcact
    11161 agttatctca ttcatcagtt tcatcgcttc aataatgcag ataacttctc ctttggcaac
    11221 ttgatcacct acttttttga aagctggttt gtcgggcgct ggttgcagat aaacgattcc
    11281 cacgattggc gaaacaacct cgattccatc aaatactgcc gattcttttt ctttcattat
    11341 ttctggagca gttgttgctt gttcaggtat gacattgact ggctccgaaa ctggcaacgt
    11401 ttcgtttgtt tgaaccatat tcatgcctcg acttgtttta tttttattca tatacaattc
    11461 aaattgacct tcacgcaaat cgaattccgt tagcgaggat tgatcaaatt gactgactaa
    11521 ttctctgatt tcattgatat ccaaaattat tcctcccagc gtttcatgca cagaaccgca
    11581 ttatgtccgc caaaccctaa tgaattactc attgcataag taaaacttgc aggtttgctt
    11641 tcgtttttga caactgtcaa gtcaatctca ggatcttgtt tttctacatt gatcgtaggt
    11701 gggataaatt gatgttctaa tgccagcaaa gttgccaagg cttcgatccc gccggctgct
    11761 cccaataaat gtccagtcat cgatttcgtg ctgctgacgt aagtatctct gaaattttca
    11821 cctaatgcga tttggattgc ttttgcttct gcctggtcat tggctggtgt acttgttccg
    11881 tgagcattga tatatccgac ttgttccggc tgtacaccag cttcttccat cgcaagcttc
    11941 attgctttag atgcaccaga accatctgga gtaggagctg tcatatggta agcgtcacag
    12001 tttgcaccgt atccaacgat ttctccatag attttggcac cgcgagcttg tgcatgttct
    12061 aaagattcaa gaactagaac gcctgctcct tcacccatca caaaaccatt ccgattttcg
    12121 tcaaacggaa tcgaaccacg attaggatca gttgattctg agagtgctgt taaagctgca
    12181 aatccagcaa tccccatctc acaaatcgtt gcttctgttc ctccagcaag aatgacatca
    12241 gaatagccat gtttgatgtt acggaatgct tccccaattg aattcgttcc gctagcacaa
    12301 gctgttacga tagctgtaca aatacctttt gcacctacac gcaaagcaat atttccagct
    12361 gccatgttgg caatcgccat tggtacgaac attggagaaa ctcgttttgc acctttttca
    12421 tgtagacgaa taacttggtt ttcgattgtt ggaagaccac cgatcccaga tccgatgatg
    12481 acgccaaacc ggtcttgatt cattttttct ttatctaaac cgctcatctc cattgcttct
    12541 aaagcagcgt agattccata gatcgaaaac atatccattc gtttgctatc ttttttaata
    12601 aaatatttat cgtaagggaa atctttgact tcacccgcta aggtaatccc tgtttctgaa
    12661 gcatcaaatt tagtgatcgg tgcgatgcca ttcgtcccct cttttaagct attcaaaaag
    12721 ctgtctgcgt catttccaat aggtgaagtg actccatagc cagtaattac cactcgattc
    12781 atttgtttgc tctccttttc ttatccgtgc atgaccaatc cgccatcgac attgatgact
    12841 tgtccggtga tatatggatt ttgtgctaag aaaactgctg tttgtgcaat atcttctact
    12901 tgtccgaaac gctgcatcgg aatttgcttt tccgcttgtt ccttgacttt gtctgctaaa
    12961 acttccgtca tatcagttgt gatgaatcct ggcgcaatgg cattacatgt aatgcctcta
    13021 gttgcagctt ctcgtgccac agattttgtt aatcctacta cgccagcttt actagctgca
    13081 taatttgctt gaccaatatt tccaatcaac ccagatacac tagagagatt aatgatggcc
    13141 ccttctcgct gcttcatcat ttttttcaat acgtgctgcg tcatattgaa ggttccaatc
    13201 aaattgatat ccagacattt tttgaaatct tctgcatcca ttcgcatcac aagtttgtca
    13261 tttgtgattc cggcattgtt tactaacaca tggatcgatc caagcttttc ttctgcttct
    13321 ttgatcattt gtcccgcttt ctcataatca gagatgtcac cggaaactcc tacgcatttt
    13381 acaccgaatg cttcgatttc ttctatcttt tcttttggaa tctctccgcg accattcaaa
    13441 atgatatttg ctccagcttt tgcaaaagct aaggccatcg ctttgccgat tcctcttgta
    13501 ctacctgtga taaatacatt ttttcctttt acatccataa cgttcctcct tatagtattg
    13561 ctattgtttc atcaaatgtc tgcttattct caacacgcag aactggtatt gttttatcga
    13621 tttttttcat aaatccgctt aatacttttc cggggccgac ttcgatgact tgttcgatgc
    13681 cgattgtttt taacttatgg atactctcat agaaacgaac aggttgcatg acttgctgtt
    13741 caagtagtcc cgcgattgtt tctttcttca ttatttccgt tgttgtgttg cttatgatag
    13801 gaaaagaagg ttcagagaat tgaatctgac ttaggtcctt cgccagtttt tttgctgccg
    13861 gttctaaaat agctgtatga aaaggcccgc tgacatttag agggatcatt cttttgaacc
    13921 ctttttcttt taatagtaaa acagcttcgt ctactgcttt ttcttcgcca ccaatcacga
    13981 tctgctgagg tgtattataa tttgcaggag atacgatgcc gtacttgcta gcttcatgac
    14041 agctttcctc gatcgtttca atcggcgcat tcatcactgc aaccattttc ccgcttccag
    14101 ctggggcagc ttctgtcata taagcgcctc tttttgctac taacgcaact gcttcagtga
    14161 aggatagcgc attactagct actaatgccg aatattctcc taaactcagt cctaaagctg
    14221 catctgggat gattccatgc tccttcaata agcgataata cgcgatgctg actgttagaa
    14281 tagctggctg tgtatattgc gtctgatcca aacgttcatt ttctgtaaag caaagttcag
    14341 ccatatcgta gccgagaatc tcacttgctt catcaaatgt ctgctttaca acggcttctt
    14401 catataaatc ttttcccata ccttgatatt gtgcaccttg gccactaaat aagaatgctg
    14461 ttttcatgtt tttctccaat atatccagaa tatatgtttg attcttccta ttcaaaaagg
    14521 ccgagacatt cctatttcag gaaatgtccc agcccccaac ctaattttaa tgattatgca
    14581 gtttgttttt ctacgtaagt cactaaatct tgaaccgttt tgattccgtc ttctgtttcg
    14641 attttcacgt caaattcatc ttcaatttca ttgatgattt ggaataaatc caagctgtca
    14701 gcttctaagt cttcttggat gtcagtagtc aatttgattt cttcagtttc ttttcctaat
    14761 tcttctgcga tgatttcttg aattttttcg aataccataa taatatattc ctccgatatt
    14821 gtttgctttt tatttttata atttaacagt ctacaactcc atgagaatac ttccccatgt
    14881 taaacctccg ccatagcctg taaatattac tttctgctgt tttcctaagg tgatctttcc
    14941 actagaaact gcttcatcca ataagatagg gatgcttgca gcagatgtat tcccatattt
    15001 atccatattg gtcaggaact tttcttgcgg catctttact tttcgggcaa ttttctcaat
    15061 aattctgagg tttgcctgat gcaaaagcag ataatcaact ggtttgtctt tcatgacttc
    15121 ttggatattg tttgcaacgt ctcgtacggc aaaatcaaaa atatctctgc cagtcatttg
    15181 caaataatac gaaccttctg aatcttctga atagaacgga tttttatttt gctggtagcc
    15241 agcggtcaat gagctacttc tggtaccgtc agctgctaat ttttctttca gcaatttttt
    15301 ctgtggattc gcttctaaga gaatcccacc tgctccatcg ccaaacaaca cagcagtcgt
    15361 acgatcagac caatcgatta gcttagatag tgtttcacca ccaatcacca gtcccttagt
    15421 tgagccgcag cggatcattt tttcagccat gcttaacgca taaacaaatc ctgagcacgc
    15481 agcactgata tcaaaagcga tagcatgagt tgctcccaat ttcccttgta cttgtgctgc
    15541 tacagatggt gtcgtgtaat cgggcgacat tgttgctact aaaataaaat ccaaatcttt
    15601 agggtttaga tttttctgtt ttatcaattt ttccgccact aaaatgcata aatcagaagt
    15661 ctcctgattt gttacacatc ttcgttcacg aatacccgtc cttgacgaaa tccattcatc
    15721 agaggtatcc atcatcttgg aaagatcatt attagtaata atcttttcag gaaggacgct
    15781 ggctgttgca gtaatcattc caaattgttc cattaaatat cctcacttat agtcttgcaa
    15841 gaagtcgtgc aagtttttca gtgcatccag caaggcatgc tgttcctccg tagccattcc
    15901 gtctaagatg ttcttgacca tttcacgatg aaaatgctga tggacccgaa atagtaactt
    15961 tccttttttc gttagtccca actttacaac gcggcggtca tcttcactgc ggatacgttc
    16021 gacatagcct ttttttacta acttgttaat agcagtggtc aaggtcccaa cagtaattga
    16081 tagttctttg gctacttctg aagttgtttt tttcttgtac atcccgattg cctcgatcgt
    16141 gtgcatctct gtgattgaca gatctttgaa ttgagatttt ttcagctcag actcttcgat
    16201 tgtcaaaata tcattgaaga cactaacaag ataatcattt actgttttta aatctggttc
    16261 catgttccac cgtccactaa ttagtttgat tatcaaatct tttgatgttc aaactatatc
    16321 ttaacttttt tcaaaatgca accttaaaag atcatttttt tactatatga gcttttttta
    16381 agattttact catttaaaac aactgataaa tcagcattta taatttcttt tattagaaaa
    16441 atcatctatc tcacatattt tgattatcaa agtattttct gtgaacagaa ctatagaatt
    16501 ccctaaaact tgtccattat ttcaaaattt gttatagtct ttttattcaa agcgtaaaag
    16561 gagatcctat gttaaaagaa aaaatgaaaa ctctccccat gctgccgggt gtctatctta
    16621 tgaaagaccg agatggtaag gtcatctatg ttggtaaagc aaaacggctc aaaaatagag
    16681 taagcagcta ttttcatcag aataaacagc attcaaaaaa agtattacgt atgattcatc
    16741 atataacaga ttttgatttc gtcgtagtag acacggaatt agatgcttta ttgctcgagt
    16801 gtcaattgat ccaacattac cgtccttttt ataaccgaca aatgaattat ttcagtaatt
    16861 ataattatgt acatattacc aataagggat ttgtattaac agatactcct acggctcgaa
    16921 catacgggcc ttttcgtctc tataaaaaaa tgccgtctat tttgcgtata atggaagaaa
    16981 cttatcaaat gccttggttg tctgaaatta gtttgttagc tttgcgagtc cagcttcctg
    17041 acctacagga aatgactttt gaacagaaaa aaaaggaact ccaagggctg tttcaaggcc
    17101 gaaataaaaa gctactgact tacttgaaaa aaagacaaca acatttcatc tatcagttga
    17161 atttcgaaaa agccggtatg cttcaaaaag atatcgaatt agttacttat tttattagac
    17221 ggatccagga acaaaagcag tttttaagaa caccatcgct caccttcagc atgccattag
    17281 cagcagatga gagtcagaaa aagcactacc tcgtttgtta tggtcaactt gcagaaacaa
    17341 tgattacctc tggtgacgtt tctcctgact tctattatga aaagaaagaa gctcatttat
    17401 ctttgaaacg tcaattgtca aaagaagaaa tcgatcctgt acaaatcttg atcagttacc
    17461 gcaaaaaatt agaaaaagaa cagtttgaaa ttgaactgtt aaacaaaaaa gaagctgaaa
    17521 agcaactaaa ttagttgctt ctcggcttct tttaggcatc ttcaacttca aatgtcaatt
    17581 atcctaaaat ttattggatt aagtcataga tttccatcgc tacgatatcg atattatcaa
    17641 attcgtattg ctgatgagta tctgtttcag ttaattcaaa tgtatcggtc gctttatcat
    17701 aagttactgt acacttctct acgccttctt tttcaaaacg gcgaacttgc agttcattat
    17761 ctgctgactc gaccatagcc tccagtcgtt ttatgattgc gactaattgt gaatgtgtca
    17821 tcggaggtcc tccttttctt atttctgtta tattgtatca aaagcatagc ctacttgcat
    17881 tgattttctc taaaaaaact cgttttttat gatttttttt ctggtatttt tccttcaggc
    17941 caccaaagtt tgatcaaagc ttgggttccg tcattctcaa aacctttatc tgccaactct
    18001 tcatatagtt tcaatgcttg ggtagttgct ggcaatacaa gatccatttt ttctgcttct
    18061 tctaatgcaa tctttaaatc tttgataaaa tgtttgacaa aaaaaccagg tgtgtaatct
    18121 tcttttaaaa tacgaggaga ataattgctt aaagaccagt tagctgcact tccacctgaa
    18181 agcgtctcaa tcactttctt taaatcaagt ccgttttttt gtgcatagac aagcatttcc
    18241 gtcattcctg tcatcgtacc tgcaatcatg atctgattag ccatttttgt atgctgacct
    18301 ttcccagcag aaccatgcaa tgtaaatgtc gtaccaaatt ctttgaataa aggcagtact
    18361 tgatcataaa cggcttcttc tccaccgacc atgatcgtca gtgtaccatt ctttgctcct
    18421 aagtcaccac cggataccgg tgcatctaac gcatctgcgc ctttttcttt tgctgttttt
    18481 gcaatttttt ctgccaggct tggtgtactt gtagtcaaat caactaaaat atgtcctgag
    18541 atatctgcag aaaaaatccc cgactgtcca tagtagattt cttctacatc tttggggtaa
    18601 cctaccattg taaagagaat attactttgt tctgctaccg cttttggtgt atctgcccag
    18661 atagctcctt ctgttactaa aggatcagct tttgcttttg tccgattata gacagctact
    18721 tcgtgtcctg cttctagtag atggcgtgcg acagcacttc ccatgacgcc tgttccgata
    18781 aaaccaagtt tcattttttc gacctcctaa aaatcaaaaa tccttgcttc tatgctagta
    18841 tacgctagaa acaaggattt tttcataaaa aatgtttgaa ttttatcttt taaatgaatt
    18901 taattttatt ttgattcgtt caatcgagtc ttcttcttct tttcttcgca tctcttcttc
    18961 atgataaaca accatgtctt gataaaattc gatcatagct tcgccgaaat gtgtagatga
    19021 aatatcataa agtttagctt ctaaaacttt tggatcttta ggaacttcac tggaaatata
    19081 atcaataagt gctggtgcaa agtcttcgtc ggtttcaaaa gttttcccta atgaaggatg
    19141 atcgaacaat cggttcaaat aagcatttcc ttccacgaca caaggtgttc cggctgccat
    19201 cgcttctgta taagtaagcc cttgtgtttc tgaagtagaa gcactgacaa aataatctgc
    19261 cgctttataa taaagtgcta catcattgtt gtccacttct cctgtgaact gcacaaattc
    19321 ttctaactgc agttctgaga tcaattcctc taactcttct acataaggtc cttttcctac
    19381 gataactaat ctggcgttag gataagcgga gatgacctga ggaaacccat taatcaaggc
    19441 ttggatattt ttctcataag aaatcctgct taaagaaaga atcatcaaat ggttttcctg
    19501 aagacctagt gactccctta atttgctgat atcctgctga gtgatatcag aacgtttgaa
    19561 tttttcaatg tcgataccag taggaatcac gcgaagcggt gctattacac catagctgcg
    19621 taatgtatca ataacacgtt cacttggaca aactacacct gtcgtatgat ttgcaaataa
    19681 tctagacaag tattttacat gggattgcct gatcactttt cctttaccga tataatgaag
    19741 gtagtcttca tacatcgtat ggtaagtatg gatcagaggt attttcaact tcttagctac
    19801 catcttccca agtatccctg ctccgaattc tgtatgggta tgaataagat ctagttctag
    19861 ttctttggca accaaataag cataccacat gcctctaaca acaactcttc gatccttgaa
    19921 agaaataaat ggtacacttg gcattcgaat cacatctttt tccaacttat ccgcattagg
    19981 atcggtcgtt gtaaaaatat agacttcatg cccatgtctt tctaattctt gttttaatgt
    20041 tttgatcgat gtagcaactc cgctgacttg cggaaagtag gtatcggtaa aaaagccgat
    20101 tttcatgttt ttcgcctcca aactagtcct tgacctgatt tacttttttt aatttgctag
    20161 agactttttc attcagtaca ctttcgtata catttttaag ttcttcaccg attttattga
    20221 tacttcgctc ttttgctgtt tgataaccgg cttgtgtcgt actcggtagt tcttgattgg
    20281 taatctgttg gatcagtcgg ataaattctt cgtttgtctt ccccatatag cagttcttgc
    20341 tatcttctaa ccagccatta tacactggaa tatctcgaac aagtacgttt tgtttgcttg
    20401 ctaaagcttc taaaacaacg atcccttctg tctcttcata agaagggaaa aagaatagat
    20461 ctgcacctga atacgcacct tctatgatct ctccacgaat gtaaccagga aacaggacat
    20521 tttcaggatg atcttctttt acaattttcc ggatgttccg aggaatcgag tacatgggaa
    20581 catgaccaaa ccaaataaat gtgtattcgg gcatcttctt agcaatttct acgaaatcaa
    20641 tgattccttt acgttcgaaa aacaaaccga cacaaatgat tactttttga tccggagaca
    20701 gtttgaaata ttctctgaac ttttgttctt tcattggatc agggtgatat ttctctaagt
    20761 cgatcccatt ggaaatagct tgaatcggca cacggatacc atagctttcc aacaactgtt
    20821 ttgaataagg tgttggcgtg atcagatagt ccgctttttg gtacaatcct accagatatt
    20881 tttttacgat aggagaaagc tggttcgatc cgataaagga atttcggaaa tcttcctctg
    20941 tagaatgcgc atgatagaca actttttttc cttctcttct ggctttattg accatattat
    21001 gactattgat tccgtatgta ttgatgtgca ggatgtcata atcttctttt tcatctagtg
    21061 tataagagat accgacttcc gtcagagcac gtttttgatg atcaagcgca cgcccgattc
    21121 cagattttgc aagcatcttt tcactttcaa aatataataa taccttcacc ttacgtcccc
    21181 cttcacttat atagtataac atagcctaca aactgtcact caaacttaag ccctat
//
LOCUS       DAJSBM010000047        21216 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJSBM010000047 DAJSBM010000000
VERSION     DAJSBM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.047"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(59..775)
                     /locus_tag="ONT67_001908"
     CDS             complement(59..775)
                     /locus_tag="ONT67_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HCR2355645.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(1002..1544)
                     /locus_tag="ONT67_001909"
     CDS             complement(1002..1544)
                     /locus_tag="ONT67_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2355646.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(1549..1776)
                     /locus_tag="ONT67_001910"
     CDS             complement(1549..1776)
                     /locus_tag="ONT67_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2355647.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(1855..3735)
                     /locus_tag="ONT67_001911"
     CDS             complement(1855..3735)
                     /locus_tag="ONT67_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCR2355648.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(3924..4538)
                     /locus_tag="ONT67_001912"
     CDS             complement(3924..4538)
                     /locus_tag="ONT67_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355649.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            4680..5240
                     /locus_tag="ONT67_001913"
     CDS             4680..5240
                     /locus_tag="ONT67_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2355650.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(5545..5859)
                     /locus_tag="ONT67_001914"
                     /pseudo
     CDS             complement(5545..5859)
                     /locus_tag="ONT67_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(6113..7748)
                     /locus_tag="ONT67_001915"
                     /pseudo
     CDS             complement(6113..7748)
                     /locus_tag="ONT67_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8303..8866)
                     /locus_tag="ONT67_001916"
     CDS             complement(8303..8866)
                     /locus_tag="ONT67_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2355651.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(8881..9924)
                     /locus_tag="ONT67_001917"
     CDS             complement(8881..9924)
                     /locus_tag="ONT67_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355652.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(10057..10461)
                     /locus_tag="ONT67_001918"
     CDS             complement(10057..10461)
                     /locus_tag="ONT67_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCR2355653.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(10694..11329)
                     /locus_tag="ONT67_001919"
     CDS             complement(10694..11329)
                     /locus_tag="ONT67_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2355654.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(11595..12647)
                     /locus_tag="ONT67_001920"
     CDS             complement(11595..12647)
                     /locus_tag="ONT67_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355655.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(12660..13598)
                     /locus_tag="ONT67_001921"
     CDS             complement(12660..13598)
                     /locus_tag="ONT67_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2355656.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(14049..14699)
                     /locus_tag="ONT67_001922"
     CDS             complement(14049..14699)
                     /locus_tag="ONT67_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355657.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(14799..15308)
                     /locus_tag="ONT67_001923"
     CDS             complement(14799..15308)
                     /locus_tag="ONT67_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR2355658.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(15509..16378)
                     /locus_tag="ONT67_001924"
     CDS             complement(15509..16378)
                     /locus_tag="ONT67_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2355659.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(16398..17519)
                     /locus_tag="ONT67_001925"
     CDS             complement(16398..17519)
                     /locus_tag="ONT67_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCR2355660.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(17521..17916)
                     /locus_tag="ONT67_001926"
     CDS             complement(17521..17916)
                     /locus_tag="ONT67_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR2355661.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(17935..18741)
                     /locus_tag="ONT67_001927"
     CDS             complement(17935..18741)
                     /locus_tag="ONT67_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2355662.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(18734..19522)
                     /locus_tag="ONT67_001928"
     CDS             complement(18734..19522)
                     /locus_tag="ONT67_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355663.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(19536..20012)
                     /locus_tag="ONT67_001929"
     CDS             complement(19536..20012)
                     /locus_tag="ONT67_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355664.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(20084..>21216)
                     /locus_tag="ONT67_001930"
     CDS             complement(20084..>21216)
                     /locus_tag="ONT67_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2355665.1"
                     /translation="KIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANR
                     IVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDA
                     QKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCS
                     CASGLGTSFKLKKILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLN
                     PHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTI
                     LNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNE
                     TSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         6580 a         4350 c         3232 g         7054 t
ORIGIN      
        1 aaataaactg agcaaggggt tgtgataaaa gacatgtcac aaccccttgt ttatctcatt
       61 aatttgcttg tccataaacg atttgattaa agccattttt caattcatta ttcgctgtct
      121 cgttgaagta aagataatta tcgggattcc cctgattttg ttgtgtcacc aatacttttg
      181 gctgattttg ctgaaacccg aatagataga cgtgcttttt cagatatggt tctgtctctg
      241 catcggaata gacggctgtg atttgatagt ctgcttgacc tgtcccactt tctgaccatt
      301 ctgcagaaac aggcactcca ccaatagcca tcttccactc cccgttcaaa atagcttgag
      361 ggactttaag accatataag tccacactca tttgattcgt ataacttttg tattgttgtc
      421 ccattgtttg gccccatttg gtcataaatg cctctaattg tgcggatttg tctgcattcc
      481 acagtgtttg ttcaacttgt tcgctgcttt ctgaagagga agatgaagcc tctacagaag
      541 aacttgaagt agatgtttga gttgtttcag tcgttttgga agactctgac gaagtagagg
      601 tatctgctgt ttctgtactt gctgcagttg acttgctctc tgtgctagtt gttgtagtag
      661 ttgcaactgt tgctttcgct gtttcttttg ttgattcttt tttcgattca ttcccgttgg
      721 tacaaccagc taacaaaaaa gctagaccaa ttgttactaa aatccttttt ttcaattaaa
      781 aaactccttt cgattactcc cacttcttta ttctatcaaa taagcaatgt gattttattg
      841 atttttcgta ttttctcatc aatctttcaa atcttctaaa aactgagaag cacccgctga
      901 tgttgtctgt aatacatcag cgggtgcttc ttttatttta atcttcgtga ttttccaatt
      961 tacctcacgc tttatatttg tatttgaaga aacatagact tttagtcatc atcgtcttcc
     1021 tcatccaaat cttgtcggac agtttttcct tggagtcttt gtacttggta gatgatttct
     1081 ttattgaaac ttaaaagttc aacaagcaat tgttgcaagc catatttttc ttctttttca
     1141 gctaatttga ttgcccgtgt gataaagaca ttttggaaat caaaatcttt tgcaaagttt
     1201 tccaacattg ttgaggctgg taaatatttg ttttgaccat gttcgtaaat ttttccataa
     1261 cgcatgactt cttctaaagt agacggaacg atttcatttt caatcactaa ctcatgagag
     1321 attttattga accatctcgc ttcttgatca gcatattctg aaaaaagggt acttaattct
     1381 tgcgaagatg tacccaaaac atacaattgt gcttggatca atttcatttc ttctaattta
     1441 aaatttgcta atacatgccc caccatggcg ccagcagttg gtttatgatg gtcgatatct
     1501 gcttgttttg tttcttgaac aaatttttct tccactatat tcattgattt acgcctcttt
     1561 tacttttgct gattggactt cataacctaa tttttccaca actgcagcta attgttcagg
     1621 gtggacttca tctggatcaa agtttgcttt gattttgctc gcattaaaca aaactttcgt
     1681 attgcttacc ccaggctgtt gagaaaccgc atgttcgatt ttttgcatac agcttggaca
     1741 agctagtgta tctaattgca taataatttt tttcatcgta gttcttcctt cctttttctt
     1801 gatagtctta ttctagatta cagcttccat aaaaaaattg attcttatca agttttacga
     1861 tttttcttct ttgctttcgt gtccaaaata caataaacgc atcgcgttaa agataacgat
     1921 caagatacta gcttcgtgaa cgaacatccc actagccatg taaataaacc cggcaaataa
     1981 gccgttgagt aaaaataaga cgacaccaat ggcaatcact acattttcac gggtatttaa
     2041 aacagttttc ttagccaagc ggtaggcatg aaccaacgat tcaaaattag attgcatcaa
     2101 gacaacgtca ctcgtttcga tagcaacatc cgttcctgat cccatagcaa tcccgatatc
     2161 agccgcagcc aaagagggag aatcattgat tccatctcca ataaaagcaa cgcgcaatcc
     2221 ttgttcttgg tattttttca caaattgaac cttttcgtct ggtaataatt cagcatggac
     2281 ttcatcaatg cctaacatat cagctacata atcagcagtc atctgattat cacctgtcag
     2341 catcactaac tgttttgctc ctgcttgttt caacttagct aattgttcag cgacttctgg
     2401 tcgaatcaca tctgacacac ctatgatttg agtgattttt ttgtcgattg ctacaataat
     2461 gaccgagctg cctgtttgtt gtaattgatg taaatcttgt acttgctttg gtgtgagaga
     2521 aagtttgtga gcggcaatca atttggtgtt gcccgcacaa actgtatgac catcaatctc
     2581 agctgtgatt ccttgtccct tgattgtctg attatctttg actgctaatt gttgaaaatc
     2641 aatgtctttc tcctgagcat aatggatcac ggcgcgcccc agaggatgat cggataacgt
     2701 ttctaatcga gccactaaag ctaagctgtc cccttcatcc tctgtatagt tcttgatagt
     2761 ggatacagca gtagttcctt tggtcaatgt accagtttta tcaaagacaa aagtatcgat
     2821 tttggaaaaa gtatccattg cttcaccgcc tttgactaag atgccccgtt tcgctccatt
     2881 accgattcct gctacattcg aaacaggcgc accaatgact aaagcgccag gacaaccaag
     2941 aaccaaaaca gtgattgcta aacgaaaatc acgactaatc agaccaacaa ccaacgcaat
     3001 aagtaaaacg gttggagtat aatactgaga aaatcgatca atgaattttt ccgcatggga
     3061 tttggtatct tgtgcttctt ctactaattc gataattttg gcaaaggtcg tatcatctcc
     3121 gaccttagtt gcttcaatcg tcacgtagcc atctgctaaa atcgttccag agaagacttc
     3181 atctccactg attttgacga cttgtcgtga ttcaccagtc actgcggctt catccgcata
     3241 ccctttcccg tcaatgactc gtccgtccac aggaatagct ccaccagttt ttactaagac
     3301 gatatcgcct tcttccacgt catcgatttc cacttcttca gtcgttccat cttcttggac
     3361 aacactagca gtagatgggg ccatctcggt caaagccttg attgactccc gcgttttggt
     3421 caatgttttt tgttccagat agctaccaaa caaaaacaag aatgtaacga tagctgactc
     3481 cttgtattct ccgatcataa aggcaccaag cacagcaata gtaacgagta aatcaattga
     3541 gataactttg actttcactg cctgatacgc atggatcata ataggtatga ccgcaataat
     3601 agaagctatg gctagagcaa tgttatagaa caagacaaaa ccagtaaatt ttccgataaa
     3661 accaaaaata atcaacacag ctgataaggc ggtgatatga tttcgatgct cttggatcca
     3721 cttttgtaat ttcatctttc tccctctttt cctttcattc ttacgagact aagtatagat
     3781 acttatccat cataaaaaat tgaccactat caagtttagt caaaaaggcg gttttctttt
     3841 ggtagaatac actacaaaaa atagttagca agaagactca ctaaaagtga tcttgccaac
     3901 tattgtcgtt tctatcgaaa ttttcattga atgattttcc tgtacagcat acatcggaga
     3961 tctatttctt tatctaagat ttttatcttg ctaatttttt cctttagtct ttcaaatcca
     4021 cgtcgttcat agaattgata cgtacagcca ctatctgtaa aaaggaatat ttcttttcct
     4081 ttctctcttt tttcaaattc tgttagcaac ttggtaccga tgccttttgt tttcaaatca
     4141 ggatttgcag ctaaaaaaat gatctctcca tctggcgaat gcttctttaa atatgctgca
     4201 agcatttgtt catttgctgt ctcgtaagct cctttacttt ctttagcaaa tatattttgt
     4261 aaaaaatcga atatcttaac atatatttgc tgtccaaaag aacgatgttt ctttttttct
     4321 ccttttatct ccgccagcaa aacacctgcc aactcgtcct ctgcatacaa agcaagaatt
     4381 tgagttgctc gattcaactc catatacaaa aaatatcttc cgtaaagatt cagcagaagt
     4441 tcattttcca tatatctctc aaagttcatt ccttcaacag caaaatagat agcttttttg
     4501 taatctttct tttctaaggg tttaaaagtt agtttcatca tttcgcctcc tcaacttcaa
     4561 tgattgtgtt atacttacat tataatagac acagtgtcta ctatctataa tcaatcatct
     4621 ataagtttgt cgattaagcg acagctttgc tttgatatgt cggataagga gataggtaaa
     4681 tgaaaaagac agatagacgc gtcaaaaaaa cagaaaaagc tttggctgaa acactatcca
     4741 cattattagt aaataaaaag attcaagcaa tcactatacg cgaactaaca gaaacggcag
     4801 atgtacatcg ttcgactttt tatacacatt ataaggatat ctatgatcta tatgatcaat
     4861 tagaaagcaa cttttttcgg gatctaaatg aaattctctc ttatgatcca gcccatagct
     4921 atgaggaact atacactcgc ttgattgact atgtttacgc aaatactttt ttatatcgct
     4981 tatttatcct caaccatggt gatatttcgt ttcagaagaa agttaaacaa gttattgaag
     5041 aaaactatct gaacatatgg ctatatgaag acaagaaaac gattatcaca gaagaaatga
     5101 gattctttac tacgtaccac gtccaaggtt gtctatccgt tctcaatcgg tgggtagaaa
     5161 gcaattacaa gtactccaaa aaagaattat tagaactctt acagaaactg aatgatcaca
     5221 tagagaaaat catgccttga ataaaaacat actaagctta gttaagaaga accgtaacct
     5281 tctcgtataa caactgaatc ttatttagcc aaattgttta ctgaagattt tttgagtaaa
     5341 atttctataa ttcctactta ttgaaaagta gtttgtagtg ctgcttctcc ctctctaagt
     5401 gatatatctt gttgtaccaa tcaaattcac ctcatcatta ataaattact tttaggctaa
     5461 tttattacaa cttataggtt gaagctgtaa ccaatttata gtcatattat atttttatca
     5521 tccagtttat tgaattcttc tcgtttacct caattcattt tcaaggggaa tcagctcatc
     5581 atctaacatt ttacgccatg ctcgcttcat ttcttgtggg tatttaaatg gtttcgcttc
     5641 tggatgtttg gctttatatc cttcgtaatc cttactgttg ataattgctt tcatattagg
     5701 aattggatct tcacctggca tatattgcag attagaagca tgtaattttt tctcagaaac
     5761 aaaccactca ttggaaaaga catgaatcaa atcatcactt acttgtgtaa aatagcgacg
     5821 tttaatgaca aagatatctt cattgctatc tagtgtacct tcatttcttt gtgcttaaat
     5881 tcaaagataa ttttctcaat aaagattttt gattccagta agattccttt actcctttta
     5941 caacatagcg ttgtcctttg ttttgaaaca tcttcttccc ctctttgcca ttaaaaatag
     6001 cccaattctc tataaagaag attaagcttc attattataa tataaagttt aaatttggtt
     6061 tattacttac tttttcttat tctctagtaa gctaatattg aaaaaaatgt aatcagatca
     6121 ttatttttat atgttgtaac ttcctgataa gaaaactaat taaaagaaag aggtaactac
     6181 ataaactaat taaaaaagct aacttcatag ttggaggata aatggaagaa acttcaatga
     6241 ttgtttctcc ttgtgttcct tcaatcgaca ctagtcctat tatcaatctc tcttttttgt
     6301 ccatttactt ttgcgactgt tccccaataa tcaaatacag gaagcgttat tttttgtcct
     6361 ttagattcat gtactgtata gactattttc gaatccatca cttgataagg catagtatgc
     6421 cattccttgc caattcttac ttcatgattc tgcaaatgtt ctttgtattg taatgcttcc
     6481 gtgggtgcat agtcataaag tcctccggga aggagtgtcg tgtaacgaat aagattttct
     6541 tgattcatta ccaagtgatc tctctgagtg acaactttat gttcatagtt tgcttttgtt
     6601 gcagcagata cagccaacag taataaaaca attgccgtta tattaactac ttttctttgc
     6661 ttagctgatg tgctcttcat gaaaacgaac gataaaatca acgagccaaa cagtacttga
     6721 aagcctaaga ttcgccaagg aaattgaatc atttgaatgg gtgtattttg taataaagac
     6781 catggaaaaa atttggtgga taagagccaa gagactaccg cactaaggta aatcactttt
     6841 tctttgcgat taaatccttt aaaaaagaag agtggacaga tcaaagaaat aataccaatg
     6901 attcctaccg taaaggttgt caggtcatta ttcatcgcac caatcaaact atccgagaca
     6961 tttaatgctt ggtgttgtaa ggtcgtttta tctggctcat taatcgattg aaccatgatt
     7021 tgtcggaaca tcggatacca aaagtatgct gttaaaagaa tcgtcattcc tactgccttt
     7081 gacaactcca caatcaaccg gatgctttct ttttttcttg tttcacttac tacaaagccg
     7141 cctaagacta gtaagccaac ggctattaca ctcataaaag tggaaagaac atgtgtataa
     7201 atgagtaagc tcatactgac tgctaatgga attgcacatg gctttttctc ataaatcagt
     7261 gcgtaagcat aatacataac tatcggtata accgtaagtg caatcgcttc agcaatagca
     7321 gaacgatgat aaatatcaat cgtacggtac ccataaaatg tatataaaca gctaaatata
     7381 aaagccgcat cgattctttt taaaaatttt agtccataat gaaacatcag catgatagta
     7441 caaacattca atagccaaac atataagata taactaacga ttaaattatt acttatccaa
     7501 taaaaaatga ctgccggaaa aaaggtcaag tagggataaa aataattcac tccgctccca
     7561 taatgattaa acgtcgtaaa attgattgga ccagaaaaaa ttgaagataa ccctttgact
     7621 ctagcaaaat ggaaggcaat atcccccgat gtagaaatag tatgaacatt tctaaaataa
     7681 ttgattgaat aaagtccagc aataattgct aggagaatgg acaatattat ttttttcttt
     7741 acttgcatta cttaattata tactcctttc atggattgct ttatctagta taacttaaat
     7801 tttaaataaa tcgttattta tttttactct cacttaaaaa agcactaaaa ttataagaga
     7861 gggaggtaat ggtctgaaca ccaagaattg ttccaacatc gaattgctta actaatgagt
     7921 tcaaagctta attatgtggc tcttatattt accgagcgtg gattgcgatc tctttagcga
     7981 aacaaacatc tttcataaaa aaaacagcca tatccgcttt cttggatatg actatttact
     8041 actactttac tatctttcat tcttcttgtc tcacttatga taaggcgctg cgtatcatga
     8101 gtgaatgata ttaggaaaac ttcataaaat aaatctttta tacatttcca cttctagatg
     8161 gtcattcatg ttagaagaat caaactattt ttttactgtc gtaataatat taaaacacta
     8221 gaacaaatca attaaattcc ataaaatata aataatagac ctagcatcat ctggtataaa
     8281 atggatgcta ggtccgttat atttactcaa aattgattct acgaatagat cttcttatca
     8341 tttctatttt agtagtcatg cgttgttgtt cttctaaagg tggtaatggt ataagtagtt
     8401 tagataaatt tgtaccattt acatttggtt gcccagttcc gtaagaaatt ttttctaata
     8461 acttccaata taatggcgag tctaagaagc agtctacgta ttctgataat aatttctcaa
     8521 ctaacctaat tcgaatcaag taagaagcaa aaacagattc ctcacttatt tccttaacca
     8581 agaaactttt tcccatagtt cctcctgtcc ttgctatcaa aatgtcattt tcttccaatc
     8641 ttaaatcaac tagtttagaa ttactaatat cacagtaagg cacactggac caatttacac
     8701 gtccctcttg tatatctgtg attctcaaaa acttaacatt gccttgtttt ttcgccgaat
     8761 ctgtatagcc atattgaatc ttagtagaaa tacttcctaa ataaacccat agtcgaacat
     8821 cgctagatga tttttcttgt cgttctttat gaacaacagt gatcatcttg agttgtccga
     8881 ctatttttta gttgcagcaa tctttttggg acgcttattt gcatgaagca acgttgctaa
     8941 gtctagtaat aaaacattgg ctaacacagg attggctttc tcgcgatacg ctgcggcgcc
     9001 actttcaaac aatactgcta gacgatcttc tacaacaacg atttgttcca aatacggtgg
     9061 aaatttgatt cgttttagga tccggctagg agagtcccgc tcggacacta agagttccga
     9121 ggtataagga ccataagaac gactggctaa gagataatgg ccataaaaag ccacaccttg
     9181 aatcttatca gatgtttttc tgctcattgt cacggttggt tggattttgt ctttgcggcg
     9241 aattgctgtt ttcatttcag ctatcgtcgg caatagatag aaagtcaact gtccttcacc
     9301 ttttagtgtg aagttgccag ataccagttg atcattgttt tgagtcaaat acgatgcttg
     9361 tggaatctct gagagatcag ttgtcagcca ataagcgatt ggctgtcccg tctcttcaga
     9421 tgtatctgct aagatatcga tcaaacgtaa agcggctact cgtccagtag cagatcctgt
     9481 gattgtcaac cataaaagtt cccgttttgt atcatatact aagcccccta catgtggccg
     9541 tttaggtaaa atcagcagtt tgatcggctg tcctgtttct ctatccagta catgaataat
     9601 agaatgatgc tgatggtcat agcagtaggc agaaataaac acaaaatctt gagaaaaagc
     9661 caacccttgc ggcgtcatcc attgacaaac ctcattttcg ccggtttccg gcaccaccat
     9721 tcttgtttct gttaatccag gaatgttata ttgttttgct tcagcctttt cccaagctgc
     9781 ttgaacaata ggataccgtt ttaacttttt ttcaatatcc tgatgtatcc gttgctgctt
     9841 ccaatgctta aactttagga catagtaaac aagaaggcca atgaccagca acactaaaat
     9901 cattattgct acccgctgtg tcattttttt gcctccttgt tctattcgat caatcgtcca
     9961 tataccgcta ttaattaccg taactacatg tttcttttac tagcgtttct ttataggatc
    10021 tttcataatc tcatttatag taacattaag aatcccttat tctactatag tcacttccac
    10081 atcaatattg cctcttgttg ctttagagta tgggcaaaat gcgtgggctt cttctgcgat
    10141 tttctctgct tggtccttct caagtcctgg caaagaaact tctaaaacga ccccgatttg
    10201 gtagccccca tcttctaatt gaaataaaga tacccgtgct ttgacttcag aatcagactc
    10261 aagattattt tcttccagca tgtgttgaac cgcgccgttg aagcaagatg cgtatccagc
    10321 cgcaaaaagt tgttccggat ttgtcccttc actatggatt cctgggcggt cgatcttcat
    10381 gtgcatactt ccgtttggtg cctcaacttc gccatcacgg cctccacgat tgatcattgc
    10441 tgtttcgtac atttttttca ttgagatcat cctttctttc gtcgttctat attaagtgta
    10501 acgagtattt tttaatctga caaaggtaat gcttgcattc ggcgacatca gttagacaac
    10561 atcgatgatc agtgatagaa gcagtttatc tatacaaaaa aagaagggtt ctatgatatt
    10621 tggtgtcctt actcgccttg agtaacaaca ccaaatatca tagaaccact taatcagctt
    10681 cacactgctt tttctacttt cgtagttgaa aaaaacttaa acaaatcatc ggcaaatagt
    10741 taaagaaaaa acttaatcgt tgaaagaagc ccaattgctt aaagaaggca aactgactaa
    10801 tcgacggaat ccgtgctaat ccgtaaagac ctgcaattag taaactaata actgttaaaa
    10861 caagataaaa atggctaagc gtaccttgac cactttgacg ataaagcaga acaagtaaca
    10921 atgggaaaag tagaaagcct gcatagccta gcccagaacc tatattatgg atccaagtcg
    10981 atagattaca agagctttca ttcgtattga tactgaataa gccagtaaaa atacaatcac
    11041 ctatcccata taaaccaact gttgcaacaa caagtattgc aagtgttttt gatgttccaa
    11101 caaataggtg atacaacgca ggcaacgaaa gaacaaaaaa aagtccagat acaactgacc
    11161 agaccaagaa agcacgtctg actggactat ctacgtcacc aaacacacta attacggtag
    11221 tcatttgatt cagtttggga taaaaaaggc caaggatata cggtgtcgat aaatcactca
    11281 tcacacctaa taataaaaaa taaaaaccat atttttgcaa aaaaatcatt ttcttcccct
    11341 actttttagt gattattcga tacatccaac aatcaaattt tttctcattt taacacatat
    11401 ttttattcta gttgtctttt gaacaatcaa taacataaag gtgagtttct tctacatttt
    11461 aaaaaatcga atggcactga agagttcagt tgtcagcatc aatctcctac tcaaaaaggc
    11521 aaaaaaacgt aagtcagatg ttgttgacat ctcacttacg catccattga ttgaaaattt
    11581 cttagtaaaa tctactagcc taaaaatttg aagacttctt ctcgatcata cagtttaaat
    11641 actttctcgc ataaaaattg agtaattagt gccgcaaaaa cagggatgac taaccaacaa
    11701 agaacaacaa gaaagccatt tagccctgca tctaaagaag ccaacggacc aacaagtccg
    11761 accacgccga aaccagcaga agcaggcgta cctgaaatag aaaatagagc gacaggaata
    11821 gctgtaatca ctgcactaac taaacaagga ataagaatca caggttttct aaatagatta
    11881 ggcatcatca tcttcatagc gcctaaagca atcgcaattg tcacacctga cttattaacc
    11941 ttccaagagt tcactactaa aacaaaagtg gttgcagcta cgcccatcgc agccgcacca
    12001 gcagaaacac catctagttg gattgccatt ccgattgcta ctgttgaaat aggagagata
    12061 atcaaaaagg caaaggaaca accaatcaag atagacatta acagcggttg taaggtagtg
    12121 aagttattaa tcaatgatcc aattgcagaa gtaattgctg ttacaaaagg gaataaataa
    12181 aaaccaagta aacctgcacc tacacctaca acaatcggtg ttaacacaat cgacactgac
    12241 ccaaaactat ttccaatcag taaaatcagc ccgacagcaa tcgatgctgt cagcatcgta
    12301 ttaataatat cgcctgtacc agcaccaacg taaacacccg tttcacccaa atcaggtgtg
    12361 aatttgacta caccagaacc aacaaaagaa gctccggcta caaccatcat tctcatcgga
    12421 tcaaatccaa attgtaaggc aatcaaacca ccaatgatca aaggtgttgc tagttgaaaa
    12481 ataactgcta actgtgcgat cgttaccgca aagggatacg cactaaaata ttttaaaatg
    12541 gcacctaata ctgcattagg aatcaatcca ataatgattc ctgttgctgt accagcgagg
    12601 actttattca gaaaaatttt cggtgatatt ttttgtgaag tttccattca atctctcctt
    12661 tattttacgt tcaaactatc ttcttttgca tggacaagct cagtcaagaa atcacaataa
    12721 ggcgtcgcaa caccatattt cttccctttt ctagaaattg cgccattgat ataatcgatt
    12781 tctgttaatc gatggttctt aatcaagtct tgatacattg aaggataatg taacccaatt
    12841 gtttccgggt caaaacaaga ttcacaatgt gcaatgactt ctgggacatc tagttcaatc
    12901 ttctctactg ctgctacttt ggcaaactca ttgacaatcg tcgccaccat tttatgagca
    12961 gtcgatgttt ttcctagctc agccatatta acgtctaaga ttgtgcatag tccgttcatt
    13021 gttccgttca cacatgcttt gcgataaata gaatagtgaa tattgtcaga aaaatgagca
    13081 tttaatcctg attcagacaa tttgtctgct aatttttttg cagctgcttc ttttccatca
    13141 cctaaatttt gtaattctac tgaaccactt ccaaataatt tgacctgacc aggaccttca
    13201 agacctgccg tccacatggt atttccaata tagatatttt ccattggtac aaatttctca
    13261 ataatatctt catgcccgat accatttaat agacataaga cctctgtatc ttttttgatt
    13321 aatgattgga tatcctgcag cattttttcc agctgcatcg ctttggtaaa tagaataatc
    13381 agatcaactt gatcttcttt ttctacttcg gattgaagaa cgattggtag ttttgcttct
    13441 acctctttac cattaaaatt tgcttgtaat ccatgttctt tgatttgttg gacatgttct
    13501 gcccagccat cgattaataa tacttcgttc ccactttggt gaagcatcaa cccaaagcga
    13561 cttcccattg cgccagcgcc tgcaattgct attttcattg attcttcctc ctttttcttt
    13621 ctattatact catttgagaa tcttttcaca acctcgtttt gttattcttc tcttatttat
    13681 catgtttata tccaccattc aagttatttt atcagcaaat caataaaaac tggaatagaa
    13741 tcccaatttc atcgtaagag aaactagact agcaataaga aaatagaaac attcatagaa
    13801 aaaatctcac tcctctgatt ataaaagagg gggcattaac ctcgtgtata ctaaaacctt
    13861 ccattcattc gatggatgat tttctttttg tgaaaaaata ttcattgtag tacgatgttg
    13921 cgcaacacag acggatggta ttgcttatta tttattaaaa tcgaacaata cacttattta
    13981 aaaacttctt tcccagattt tcttccattt ttacagttat aactagtatt atcatgaaaa
    14041 atagcctttt attgtctatg ggaataataa tttaaaattg gtactttttt attaattagt
    14101 tcatcgccca ataatctctc cagtttctta gcatctcctg actcaaccgg aattatccaa
    14161 gcccagtcta aataattttt taaaatctca tcatattttg caaatatttc attgccatat
    14221 tccgtcctta aaattatatt ttcagtgcca gacaagtgat ttatattgag ttgataaaaa
    14281 agatctccat ctaaaaaaat atcattagaa gaagatttcc ccacagtaat gaccattcca
    14341 tcagctctat gtgttacata caaataattg tatgacctca tttcacctaa tgaatcaatt
    14401 atttcttgcc ctttagctct aatatattga tctctattac ctatctcact cttatttaac
    14461 aaaagttttt tcatgtcaaa tttaatcggc tgttttattt ttgtaaattc gcttctacta
    14521 ctaatccaat ccgagattaa tttttcaaca tgtcccagag taaactctga tttattcgct
    14581 aaatactttt tttgttgtat taaagacatc tttccccgaa gctcaaaatt gagcctacat
    14641 cgattctcta tctcttcaaa aagcatctta tcttcatcat tcttatattc atcattcata
    14701 aaaccaatcc agctcctcga ttaagataat attttaatct catacaaaaa ctagctctca
    14761 tcaaaacaac cattgttttg atttctcatg aaatttcttt atacttttct ccgattataa
    14821 attcctaacc ccttgagaaa attacgcata tatttatctc cgcaaggctt aaaatttcgt
    14881 tggccttctt ttctcaaaaa tgccccgatt tcgccttttg atggatagat ttctgcctca
    14941 gcaaaaacgt ctaaaatatc atcagtggtc atagacaagg cgatttttaa tttttttagc
    15001 aacacattgt tgatatattt gatttccttg cttttttgcg gcgtgtctac tatcggctta
    15061 tctttttgcg gaccacgcgc caagagaatc aggccattta ggaatgcgtc aaaaacttgg
    15121 ttgttgatgg ttttttcata cacattattt tctgcattat ctttgttctt atcctgtact
    15181 ttggtcagta ccgcctgagc ttcttcttta gtcagtggta caccgcctag ctcaaaagct
    15241 ttaatcaaat ccgtatcttt caaatcaatc gcataacgca atcgcaataa cctatcattg
    15301 ttattcattt gttcctccga agtatttagt ctttactttc ttacgtactt tttgtcttgc
    15361 taagattttt aggatcaatg ataaaaaccg catcacttat gataaggcgc tgggtatcat
    15421 gagtgaatga tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata
    15481 agcattttat tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag
    15541 ttaagcatcc tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga
    15601 tatttggcgt atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt
    15661 tcagatgttc ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca
    15721 gggtcgtatt acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt
    15781 ctatagaaag atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca
    15841 acccttcgag acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc
    15901 tcttaactaa cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt
    15961 gtatagctcc tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt
    16021 ttccatgacc tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa
    16081 ttggaatttc gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt
    16141 ttggagtaga ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa
    16201 atgaccctac tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca
    16261 aactcattgt ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat
    16321 catcattgcc aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt
    16381 cttactccac cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa
    16441 aactcatgtg gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca
    16501 taaagcttga tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa
    16561 ctaatggggt cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca
    16621 aaaccatgaa ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct
    16681 cttttagatt gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt
    16741 tctccaaagt tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta
    16801 gatgttattg tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc
    16861 caccagtatc tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct
    16921 ttgattccat caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat
    16981 ttagcatgaa aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa
    17041 aaaatgtgct taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga
    17101 aatggcatct caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa
    17161 ttcacttcgc atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca
    17221 gaaggagtgg attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc
    17281 ccagcgattg ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc
    17341 atttctgcta aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc
    17401 ccaattccta gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct
    17461 ggtgaaatag ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt
    17521 ttattcgtcc tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg
    17581 tgcctccgca ataattgctc caatatctaa attatcattt cctaactgca aagcgattga
    17641 cattgccaag ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa
    17701 aaacttttga tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt
    17761 atccactaac tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa
    17821 gctactaatt tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc
    17881 tagtattgaa tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc
    17941 taatattcca aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt
    18001 catactgatt tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat
    18061 taatgttggt aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt
    18121 gaatttaagt ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag
    18181 ccccaatact gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat
    18241 cttcattccc tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat
    18301 gattaaaatg aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc
    18361 aattgatccc atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat
    18421 tggacccatc gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt
    18481 ttcctccatt gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta
    18541 atactgaaag tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct
    18601 taaaataggt gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt
    18661 acaaactgac atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata
    18721 tttttcgctc attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag
    18781 cagtgatttg tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta
    18841 tgatcgccgc tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta
    18901 aataactaat gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag
    18961 gcaacatttt tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa
    19021 cgtaattaac agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata
    19081 aaccaaagaa aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat
    19141 tcgcatattc ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta
    19201 gttgcacccc taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct
    19261 ggctaccaaa tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac
    19321 caaaaccggc aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt
    19381 ttatatcacc catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga
    19441 tcatcggaat cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa
    19501 tttgaaaaat agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc
    19561 tgtgccttat ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt
    19621 ttatacgtgc tgatattttc atctgatgca taggctctag aagatagctg taatgcatta
    19681 tctttaacaa cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa
    19741 tgaaggattg tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc
    19801 tttttaaaat tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt
    19861 ctagctaaac gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc
    19921 atcactctgt ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa
    19981 cggttatcaa ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt
    20041 cttacaagca attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat
    20101 aatcataaac gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga
    20161 cactgaaaga ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga
    20221 aataaatttc atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt
    20281 gaacatataa tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga
    20341 tgtatatagc atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct
    20401 ccattaaatt cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat
    20461 tatcattaaa gtgtctattc aattgattgg agaatttact tatattcatt cctaaaatca
    20521 tatcttcaat tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa
    20581 aaagaatatt gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat
    20641 agtcacacgt ttctatgaca agatcagtat cccttggaaa gctatcttca agtatctttt
    20701 ttaatttaaa cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa
    20761 tatttgatga ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct
    20821 gtttaagcgg taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa
    20881 cattgtttag tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct
    20941 ccagtgaacc catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt
    21001 atctattaat tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt
    21061 cgtatatata tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat
    21121 ctccatgagc caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact
    21181 taaataaaat aaccaaaaca tttttttgaa tttttg
//
LOCUS       DAJSBM010000048        21057 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJSBM010000048 DAJSBM010000000
VERSION     DAJSBM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.048"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..152
                     /locus_tag="ONT67_001931"
     CDS             <1..152
                     /locus_tag="ONT67_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355666.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="ONT67_001932"
     CDS             complement(230..763)
                     /locus_tag="ONT67_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCR2355667.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT67_001933"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="ONT67_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR2355668.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT67_001934"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="ONT67_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR2355669.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT67_001935"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="ONT67_001935"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR2355670.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="ONT67_001936"
     CDS             complement(4488..5066)
                     /locus_tag="ONT67_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCR2355671.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="ONT67_001937"
     CDS             5388..5858
                     /locus_tag="ONT67_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCR2355672.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="ONT67_001938"
     CDS             complement(5966..7192)
                     /locus_tag="ONT67_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2355673.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="ONT67_001939"
     CDS             complement(7946..8146)
                     /locus_tag="ONT67_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2355674.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="ONT67_001940"
     CDS             complement(8280..8888)
                     /locus_tag="ONT67_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCR2355675.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="ONT67_001941"
     CDS             complement(8973..9443)
                     /locus_tag="ONT67_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR2355676.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="ONT67_001942"
     CDS             complement(9491..10405)
                     /locus_tag="ONT67_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCR2355677.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="ONT67_001943"
     CDS             10551..11354
                     /locus_tag="ONT67_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCR2355678.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="ONT67_001944"
     CDS             11532..12479
                     /locus_tag="ONT67_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR2355679.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="ONT67_001945"
     CDS             complement(12547..12840)
                     /locus_tag="ONT67_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCR2355680.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="ONT67_001946"
     CDS             complement(12868..13533)
                     /locus_tag="ONT67_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCR2355681.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="ONT67_001947"
     CDS             complement(13673..14305)
                     /locus_tag="ONT67_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355682.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="ONT67_001948"
     CDS             complement(14312..15379)
                     /locus_tag="ONT67_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2355683.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="ONT67_001949"
     CDS             complement(15376..16107)
                     /locus_tag="ONT67_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCR2355684.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT67_001950"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="ONT67_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR2355685.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT67_001951"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="ONT67_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCR2355686.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="ONT67_001952"
     CDS             18270..19439
                     /locus_tag="ONT67_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCR2355687.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..20620)
                     /locus_tag="ONT67_001953"
     CDS             complement(19706..20620)
                     /locus_tag="ONT67_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355688.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEIIFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(20678..21057)
                     /locus_tag="ONT67_001954"
                     /pseudo
     CDS             complement(20678..21057)
                     /locus_tag="ONT67_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6464 a         4141 c         3331 g         7121 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagccta
//
LOCUS       DAJSBM010000049        20418 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJSBM010000049 DAJSBM010000000
VERSION     DAJSBM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.049"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            14..1093
                     /locus_tag="ONT67_001955"
     CDS             14..1093
                     /locus_tag="ONT67_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2355689.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1290..1991)
                     /locus_tag="ONT67_001956"
                     /pseudo
     CDS             complement(1290..1991)
                     /locus_tag="ONT67_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2005..2929)
                     /locus_tag="ONT67_001957"
                     /pseudo
     CDS             complement(2005..2929)
                     /locus_tag="ONT67_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3046..4125
                     /locus_tag="ONT67_001958"
     CDS             3046..4125
                     /locus_tag="ONT67_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCR2355690.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4157..4750
                     /locus_tag="ONT67_001959"
     CDS             4157..4750
                     /locus_tag="ONT67_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355691.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4860..5087)
                     /locus_tag="ONT67_001960"
     CDS             complement(4860..5087)
                     /locus_tag="ONT67_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2355692.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5190..7778)
                     /locus_tag="ONT67_001961"
     CDS             complement(5190..7778)
                     /locus_tag="ONT67_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCR2355693.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7798..8457)
                     /locus_tag="ONT67_001962"
     CDS             complement(7798..8457)
                     /locus_tag="ONT67_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2355694.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8506..9009)
                     /gene="trmL"
                     /locus_tag="ONT67_001963"
     CDS             complement(8506..9009)
                     /gene="trmL"
                     /locus_tag="ONT67_001963"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR2355695.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9230..10096)
                     /locus_tag="ONT67_001964"
     CDS             complement(9230..10096)
                     /locus_tag="ONT67_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2355696.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10205..10846
                     /locus_tag="ONT67_001965"
     CDS             10205..10846
                     /locus_tag="ONT67_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR2355697.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10908..12269)
                     /gene="mgtE"
                     /locus_tag="ONT67_001966"
     CDS             complement(10908..12269)
                     /gene="mgtE"
                     /locus_tag="ONT67_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCR2355698.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12287..13186)
                     /locus_tag="ONT67_001967"
     CDS             complement(12287..13186)
                     /locus_tag="ONT67_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCR2355699.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13188..13985)
                     /locus_tag="ONT67_001968"
     CDS             complement(13188..13985)
                     /locus_tag="ONT67_001968"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCR2355700.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13963..14667)
                     /locus_tag="ONT67_001969"
     CDS             complement(13963..14667)
                     /locus_tag="ONT67_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCR2355701.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14798..15373
                     /locus_tag="ONT67_001970"
     CDS             14798..15373
                     /locus_tag="ONT67_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCR2355702.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15491..16168
                     /locus_tag="ONT67_001971"
     CDS             15491..16168
                     /locus_tag="ONT67_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCR2355703.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16247..18055)
                     /gene="pepF"
                     /locus_tag="ONT67_001972"
     CDS             complement(16247..18055)
                     /gene="pepF"
                     /locus_tag="ONT67_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCR2355704.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18231..19229)
                     /locus_tag="ONT67_001973"
     CDS             complement(18231..19229)
                     /locus_tag="ONT67_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCR2355705.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19455..20108)
                     /locus_tag="ONT67_001974"
     CDS             complement(19455..20108)
                     /locus_tag="ONT67_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCR2355706.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5908 a         4441 c         3639 g         6430 t
ORIGIN      
        1 aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg gaaaacatct
       61 ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt ccaaccgtca
      121 ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg atcgcggaac
      181 catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct actattctgt
      241 ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact gtggcagacg
      301 ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa tgttaaatga
      361 aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt tcgattgtgc
      421 catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca tgcgaacaaa
      481 gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc caggacaccg
      541 tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag ttgaaacacg
      601 ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca agtcggatgc
      661 cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa agttgaacgg
      721 gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg ctggcgctaa
      781 cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt ctgggctaca
      841 cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt cttgggagcg
      901 tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg gaagcccgat
      961 tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg attacccgcg
     1021 gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca agcaagaacg
     1081 gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat ggcttcatgt
     1141 ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag ttcattatca
     1201 aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga gttacctgta
     1261 gtggctaact taaacttgaa atttacggac gtaaagcttg ctgccaaatt agcgattgaa
     1321 actaacgatt gctttctagg aatcaaggac aagcgaatac catttgacga ttgatttgct
     1381 tgctgatcct gtacattgcg gaccccccac atcgtcccgt agagaatcgc cattccaatc
     1441 agagtgaaga aataaaaact tttgaaactc atattacgac ctgcatgaac ttcttgaaaa
     1501 gatacttctt gttcaaaaac agtcatgatg ggcgcgagat caacgttctc cacttctgcc
     1561 actttactcg aaattatacc aatacgctgt aaaaattgat ccatgacgtt tttcatcaac
     1621 gtctgactca ttcctgcttg acccacatgg agcgaaacag atgaaccatc aatgagataa
     1681 aaaccagcca aatcatctgc atttagcttt tcattcgctt ctacttccga tagttcctgt
     1741 acttcaaata atggtttatc attgacttcg acttgttcta agactgtggt aaaattttcc
     1801 gaaagcgctg tttgatccgc tttgattaca attccagtat taatcgtttc gagattgcca
     1861 ccttgatcaa tctcgccaaa ggccgccata aacatcaatc ctaaaagaat tggaaaggcc
     1921 aatgtccaaa acaacatgga tttatttcgc agcagtattt ttgtgcgata tttataaaaa
     1981 tgccaaaaca ttatttcccc tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa
     2041 gacatcattc aaggacgctt ctttggttgc tatttttccg taactgatct tttcttcttg
     2101 taagattgta agaatcggca acaatactgt atttagttca gtagttgtta cttggagctg
     2161 acctttttta taagcgattc catcagaagc tattgttcgc tgacaacacg ttttctggga
     2221 gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt agagattctt
     2281 tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg caaagaattt
     2341 ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga ttcagctcct
     2401 tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc ggctcatcta
     2461 aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc
     2521 caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact aattgaatca
     2581 cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg caaaaataat
     2641 cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga acgaccccaa
     2701 tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat ttaatggttc
     2761 ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc ccactgccgt
     2821 ttggtcctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa tgattcaatg
     2881 ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc atgaaaaact
     2941 ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa ctagtgacga
     3001 ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca taaatctcgg
     3061 ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt aagtcaagga
     3121 ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg tgtcagttat
     3181 ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt atgtgccttt
     3241 ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc
     3301 caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg taatcatccc
     3361 cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt acgaaaaaaa
     3421 gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac tcattgggaa
     3481 aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga aacacaatta
     3541 gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga caatgtcggt
     3601 catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca
     3661 aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat ggatcggatt
     3721 cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc cttagagttt
     3781 ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga aggattgaat
     3841 gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa
     3901 catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt ttatcgcttg
     3961 caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat tggtttgaaa
     4021 acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca agaaaaccag
     4081 caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca gtagtcatta
     4141 ttgagatgat ccatttgtga cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat
     4201 ccgtgtgtta ggcattggtc attgtgccaa ggatgcgcta gtcttatacg aaacccatcg
     4261 tcccgatgtt ttgctgacgg acatccgcat gcctaaacaa acgggcatcg atgctgccaa
     4321 agaaattttg gctaaatttc ccaaagccat ccttttgctt ttgacgacat tcaaagatga
     4381 agaatacatc cgcgaagcat tttccatcgg tgtcaaaggg tatttaatca aacagaactt
     4441 gcaagcgatt attccttctg tcaaatccgc ttataatgga caggccgtat ttggtaatga
     4501 aatcatcgag actttcactc aaatgatgaa gaataaaccg acgattgata gtcatgctta
     4561 tgcttctttt agtgaacgtg agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa
     4621 taaagaaatt gcagatgcct tgtacttgag tgacggcact gttcgcaatt acatcagcca
     4681 attactagaa aagctagatt tacgggatcg cacgcaattg gctatctatt tttatcagca
     4741 tttgcaataa gtcttatcta acacaaaaaa acctgaatga tgctccttcg catcattcag
     4801 gtttttttca catcttctat tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat
     4861 caaggattcg cagggctgcc aggattcgtt ggattgttcg gcgtgttagg cggaaaaata
     4921 ggcgccttgt ctaacgtttt ttctagtttt gattcttcac gatcgtctcg ttctacatca
     4981 ggactttcat tcaatttgaa ttctttttcc atctcatcta actttttaaa gtcattgcca
     5041 gattcttttg ctaatttcgc taatttatct tcggtttctt tagccataaa gtaacgcccc
     5101 ccttttgatt tcagcttagc atatcacact gaaaaaacaa aagaaaacgc taaccaacga
     5161 tcatttttag ctatttctca tctttaaaat caagcatttt ttgccggtat aaaatcgtag
     5221 ggagaactgt tttccatacc aatcatcgga tctactaact ggttatcgat cacttctgct
     5281 gtaagaacga aatctttaga aacagcaggt tctacctctt ctttcttttc ttctccaaca
     5341 aatgaagaaa acagatctgg ttccactgtt tccattactt ggtttttcgt ttcattcgtc
     5401 ccactgtcat cttctacagg tagcttttct tttacaaccc gtggctcttt tgtatagttc
     5461 ccatcttcaa atggatcatt tccgaccaaa gaatcttcat aagctgctag tttggtccgg
     5521 agagtgatat tcattttatc attatccatg atccttgatt tcaacgtagt catccggctt
     5581 gctgattcat tcgctacaca agtgacatag gcttgctctt cccctaacaa aatcaacagt
     5641 tccttactgc gagtcactgc cgtgtagagc aaattacgct gaagcatccg ctgatattga
     5701 tggaccatgg gcaaaataac catcttgaat tcactgcctt gtgatttatg gattgagcaa
     5761 caataagaaa gagtgatttt attccattcg tttcttttat aagtgacctc atttgcatca
     5821 aactggataa ctaattcatc cactttatct tcagaatctt ttgccaagat gatacccgtg
     5881 atttctccca tatctccatt gaatacattc aattcgggag tattgactaa ttggaggact
     5941 ttgtctccta tccgataaac ggtgtcgttc caatgtactt ccttttttcc aggtttccct
     6001 ggattgaata tctcctgcat cattttattt aacgcatcga tccctgcagg tccccgatac
     6061 attggtgcta atacttggat atcttgtgca cggaatccct tatttttcgc tttttccaca
     6121 attttagaga tcaagggttc aatttgataa gcatcacttc ggaaatagga acgatctttt
     6181 tgattctttg tgaaatcttt tggcagacgt ccttctttga tctcatgggc aagaggaata
     6241 atactagaac catcaccttg ccgataaatc tgattgagtt ccattttggg aatttgttcg
     6301 atttccaaaa gatcatggag tacttgacca ggaccgacag agggaagctg atctttgtct
     6361 cctactaaga taacttgcat attcgtcgga atcgctttga ataatgtgtt agccagccat
     6421 gtatcgacca tagacatctc atcaacaatc aaaagtccgc cttctaattc tttcgtactg
     6481 actgaaggtg ttttttcccg tccattcagc cccagaagac gatggatcgt actggcaggc
     6541 agacctgtcg tttcgttcat tcgtttagct gctcggccag ttggtgctgc aagtagaata
     6601 ggaaaaattt cttgggtata ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa
     6661 agattgacga ttccgttgat cacagtagtt ttccccgtac ctggtcctcc tgttaaaata
     6721 aatagagggg aacggattgc ttcagcgata gcttgttctt gcgaaggtcc atactgtatg
     6781 ccgaatattt tttcgatacg ccgaatattc ttctgcactt ctttttcagg ataatggatt
     6841 tcttttttac gactgagaag gcgctggatc gaattaccga tcccccattc agaaaagtac
     6901 aaactgtttt cgtaaagatt agtctcttct tgttgtatct ttccttcttc aacaagttgg
     6961 ataattacag tagcaacctg atcaggctgg atttcaattg ggcgacttgt ttctaaaata
     7021 cggatcgttt gctgaagcaa tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca
     7081 tgctgaaaaa tctgatgcaa gatagctgct cgaattcttc tcggtgaatc tgctgcaata
     7141 ccgatttgtt cggctagatt atctgcacgt ttgaacccga ttccttctat gtcttcgact
     7201 agctggtacg gattttcttc gatgatatcc aaagactcgt tcttataggc ctggtagata
     7261 ctgaaagcta actgactacc gaacccgtaa cgattcagtc ccatgatcac ttgatccatc
     7321 ccgtggttta gccgaatcgt ctcagcaagc atttctcttt ttggcttcgt caaaccggag
     7381 atttgtttta ataccgaagg atcagcaatg attttttcga tagcatcttc tcccagcaat
     7441 tctacaattt tttctgctgt tttcttcccg attcctggga atttttcgct ggaaagatac
     7501 gctacgatcc catttgcagt tgttggcttt tcttgctcat atctttcgag tttaagctgc
     7561 cttccgtatt taggatgatc cactaattct ccataaaaac gataagtctc attttcttga
     7621 atatcgccga aacttccggt gactacaatc tctttttcac ggtaatcagc tgtggtttca
     7681 ctgatgtgta ccaaaagcac tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca
     7741 actgtaccaa cgaaataagg catttcttct ggctgcatcg gaagccgcct ccttcttcta
     7801 tagtaaaggt tctggtgctg tttcagttcc taaaaaatct gtttggttcc atactttcag
     7861 ttcatatggc aataatctgc aaggctcttc agtctctaaa atcgtcaaac tactattgaa
     7921 caatccaccc atttcacgca actcacttag ctcttttcct gtcatccatt gaatggctgc
     7981 agttaaagaa gctccatgtc ctacgaataa aagtgggcca tctttatgtt gggcaaccgc
     8041 atctgctaca acagtttcga tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg
     8101 agaaggatca tacagatcta aatgattacg caaattcggc agattatccg gatatttctg
     8161 gtacatttca tcgatcgact gaccttctaa agtacccagc cctagttctc ttagcttatc
     8221 tgtataaaaa atctctaccg gacgagcaag ctctttgttg ataccttctg ctgtatcacg
     8281 tgctcgcttt gctgtactgg aataaattgc tttaaaagga acatatttta cctttttccc
     8341 aaatgctttg atttcttcgt aactttgtgg caaaagcgga gaatcgccat tgctcccttg
     8401 aaaacgtctt tcctgattcc attgtgtctt cccatgtcgg gtaaaatata gttccatacg
     8461 cagcaaactc ctgtctttct tttattttac tctataataa actgtctaat ccagcttgtc
     8521 attttcatag tgatgcgtca attctaaatt agggaatcct tgctggcgaa gtgcttcata
     8581 cactatcatt gcagctgtat tcgataaatt caatgagcgg acatgctcat cattcatagg
     8641 gatgcgtaaa cacttttctt cgttttcacg cataaatgtt tctggcaaac cggttgtttc
     8701 ttttccaaat aaaaagaagt gctcttctcc atcagcaaaa ttttcgtcac tatatacttt
     8761 atgggcaaat tttgagatca aatgtagttt acgattacct aaaaatgtta aaaaagcttc
     8821 taaattactg tggtaagtaa tatttacatc attccaataa tccaaccctg cccgttttag
     8881 atgtttgtca tcagttgaaa aacctagtgg ctcgattaaa tgaagcggag tattcgtggc
     8941 tgcacacgta cgggcaatat ttccagtgtt tgcagggatt aatggttcaa ataaaacgat
     9001 atgattcatc aggatcattc ctctcatatt tatgttcgtt tctattttct gacaatttga
     9061 tttttgcata aaaaaaaacg tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt
     9121 gaagctcttc tcactaactt ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca
     9181 acttgttgct aatataacac cctcaacttt tttttgcaac ctctactcat taaaaactgc
     9241 tagaaaaacg ttttctctta gttcctttta ataattctat gtcaattgta atcttcttca
     9301 aaggattctt atataagtct gctttgattc gctcttctac ctgccattct aaaggtgaca
     9361 tggccaaaag atctttttga ttttcctctg gtatctcaaa ttcataggtt acacgctgac
     9421 gttcagttaa tttcattgct tcagcaaact tggctactac acgttcattg gaataactct
     9481 gtttttcagg ttgattcgga taaaaagcct gacgtagttc ctttaaataa ttttcttgtg
     9541 gcaccacctt caacacttct ccatcttctg agagtacacg ttggaattct tggtaatgag
     9601 aaggcgaaaa gatatttaaa accgcgttga atgtttgatc agcaaacggc aaattggtca
     9661 aatcagctac acaccaaaat gcttcggcag attgttctgt agctgcgtag actccttctt
     9721 ttgaaatatc aaatccaaca gcattttgaa tctttttcaa ttttgaaagt tcattcaaga
     9781 agctaccttc tccgcagcca acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa
     9841 tagtctcaat aacgggctga tacatgccag attggatcat tctacgcctt ggaacgaaca
     9901 ttccttgatc gtattctgtt ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg
     9961 aaaggtcaaa acggtgatta tttttgcata tcaagccatt atcagacttt tttacttctt
    10021 cttggcaaac cggacagcgg aacagctgat gattttctgc caaaaactgg cgtcctcggt
    10081 caattttttt taacatagtt ccccctcatt tctgttttat cttaccgtta gatgctcact
    10141 ttggcaactg ctaaaaatat gatacaatgc ctagaagaaa gtgaaagata aaggagaaga
    10201 ttagatgatc aaagaatttt gcgctgaaaa ctacacatct attcctctag caattgctag
    10261 aggagccgat cggatcgaat tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac
    10321 tggtgtgatc gaagaaacgt taagttatgc tggggaaaaa gatatccccg taatgacgat
    10381 tattcgtcct agagggggcg acttcgtcta taacgatatc gaattgaaaa tcatgcatac
    10441 tgatttgatt gaagcaaaga aattagggac ggatggcgta gtatttggtt gtcttactcc
    10501 ttcaggctgg ctagacgaag aggccttaga actattgatc gaaacagcag aagggttgca
    10561 gatcaccttc catatggctt ttgacagtat tcctgaagat cggcaattcg aagcaatcga
    10621 ttggctggta gaacatggtg ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac
    10681 tattgtagaa aatttcccac ggcttaaaga attgattgcc tatacagatg gccgcttaat
    10741 cattttgcct ggtggtggtg tcactgcaga gaatgcagca catgtagcta aagaattgaa
    10801 cgtaagcgaa gtacacggaa ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa
    10861 aaacatttgc aacaggcata atgcttattg caaatgtttt tgttttttta cataaaatag
    10921 cccataaata aagaagcaat attgaaataa atcagtacac tcgtcaaatc gctcaaagtc
    10981 gtgataaagg gaccgcttgc tactgctgga tcaaatccta gacgatccat tagcatcggg
    11041 attaaactac ctgcaagatt cgccactgtg attgcaaata gcatagccat ccctatgaca
    11101 aaccccaaga taaaattatg tttccaaaaa cctacgatta gaaaaatcgt gatccccgta
    11161 acagctcctg tgacaacgcc ggtcaagact tcagccaaaa caagtcgaac aaatccatga
    11221 tctttctcat cagaaatcgc aagtcgacga acagccacag ctagcgactg ggttccagca
    11281 ttaccggcag ttcctgtaat cagcgaaatg aagactgcta aaatactcgc ttcacttacc
    11341 aagtcttcat agtgactgat taaagaagcg gtcgacatac ccaaaaacaa cagagtgatc
    11401 agccaaggca aacgtcgaga cgctgccttt actggatttt cattaatttc ttccacattg
    11461 accccggcaa gtcctgaata atcgctagca gcttcatcat caataacgtc gatgatatca
    11521 tcgactgtga cgattcctaa caaatgatca tcataatcgg taacaggaag agctaagaag
    11581 tcataatctc taaatgtttg tgccacatct tcttggtcgt cgcctacatg aactgaaagc
    11641 actcgttcgc tcatcagatc agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc
    11701 agagaaataa cccctactag ctgaccttct tgattaacga catagatgta atagattgtt
    11761 tcagctacat ctgcttcgtt cttcagcacg tacatcgcag agcgaacagt ttgattggct
    11821 acgatcgaaa caaattcagt tgtcatgatc gcgccagccg tttcatcctc gtaatggaga
    11881 agttccttga tttcactggc atcgtctgcc gacatcaaac ttaaatattt agcaatttgc
    11941 tttttatcca gcgtattaag caagtcaaca gcattatctg tatacatctc agaaagcata
    12001 tcagctgcat agctaggacg catttcatct aggtattctt tcatatgttc gttgtcttct
    12061 tcaatcacat cgaacatatc cgctaattct ttaggcgaca gataggaata gatcagctgc
    12121 ctgttctctt ctgtaagaga ttggtagaat tgcccctgct catagatatg catctctaaa
    12181 aaactgtttc tgaatgcctg aatattattt tcccttaaat cttgagtcaa ctgggcaaat
    12241 ctttcctcta attcttgatt ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc
    12301 gtctcacaac tgcctccatg tctgcggcta aaggctgctt gaactccagc aactcttctg
    12361 taaatggatg gtaaaaacgc agatcataac aatgaagggc ttgtcttttt attcccagat
    12421 ccattcgccc gccatacata tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa
    12481 ctcttatttg atgtgtcctt cctgtatgca gctggatatc aactaacgcg atttctgtta
    12541 gtcgcttctc tatccagtac tcagtctgtg ccgaacgtcc ttcatcagag accattcgct
    12601 tcaatagcga agaataatct cgggcgatcg gcaaatcgat atgtccatgt gccgataaat
    12661 ctgaaagcct tccactgact aaggcctgat attttttcac aaacttcttt tcacgaagtt
    12721 gcatatctaa ttttgcgtgc gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat
    12781 cccgatctag tcgcgtcacc acatggatta cttggttttc gtaaccttgt tttttataat
    12841 aagcctttac acgattagcc attgtcccat ttggatggta ttgggcagga atagacgaaa
    12901 cgcctgctgg tttatttact accagaacgt gttcatcttc atacacgata tcgatcggtg
    12961 tttcatccaa aagtacggtt tcatgttctc cttcagcagg aatcacaatg gttaccttat
    13021 catccttagc tagagaaaac aagacatttt ctacttgatc attaactttg atttgaccgc
    13081 cttgaaattt tattttcgct aacagaccct ttgagatccc tttttctttt aaaaaatatt
    13141 ttacttgctg agaatgatct ttctcataat cccatgtaaa ctgcatatca atcctcacta
    13201 ataaaggcat ctttcacacg atgccaaaaa tgcatatgtc gataagaagc aaaatggatc
    13261 cgttcttctg ctattcgata ataaattgag cggatatttg cttttgcgac attcaattga
    13321 tcaaccgtta caagataatc ttctgtattt tccaatttta tttctagcca ttcatcatgg
    13381 gcaatcacga tcggagaacc caaagtacga aacactcgat tattcagtga agcaatctct
    13441 gccaactgaa aggcattgat actaggatgg atcacagcac cgccgacact tttattatat
    13501 gctgtcgaac cagtaggcgt agaaatcgtc aatccatctc ctctaaaact ttcaaacagc
    13561 tcgtttttga tgtatacatc ggctaccatc gtacgattgg cccgtttaat cgttgattca
    13621 ttcagtgcta aaaaatgctg gtccggtgtt tcatccaaat aactgatccg aacatccagc
    13681 aagggataac taacactttg ttcccggttc gttcgtaagc tgtctactag ttcttccagc
    13741 tcgtaatccc gccaatccgt gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc
    13801 acatcattca gacaatgact gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg
    13861 actgagatga ccaattctgg ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt
    13921 gcgttcaaac gttctgtcac ttctatagtc tttgcttctt tattatgaac gatcgctact
    13981 ttcatcccct gcctcctctc tttcgctttc tttcttcaaa agcgttcgat agtagacatc
    14041 atctgatttt cctcttccat gggagaataa ccgctgtgct tcttggattt cttcacggat
    14101 actggacatc tcttcatcca actgataggc agcttctgcc gcacgctgaa gtcgttcact
    14161 catttcttct ggaaaaacgc cttgatattt ataattcagc gaatgctcaa tcgttgccca
    14221 gaaattcatc gcaagagtcc gaatctggat ttccgccaaa attttcttct caccgctgat
    14281 cagctgtaca ggatactcga tcaccagatg ataagaacgg tagccacttg cttttttatt
    14341 tgtgatgtaa tctcgttctt ggagaacttt catgtctttt cttttacgga tgatctcaac
    14401 aacttgatgg atatcttcta caaactgaca catgatcctt aacccggcaa tatcagacat
    14461 ttcttcttct aaacgtccca ttggaatatg ccgtaattgt gattttgtta caatactatc
    14521 gactggcttg actctcccag tgacaaattc aatcgggaca tgtcgatttt gttcacggaa
    14581 ctgtttgcgg atcccccgca gtttcacttt caattctgat acagtttgtt catatggtgc
    14641 taaaaatgct tcccaatctc gttccatagc tgatacctct acttcattaa cattcattgt
    14701 ttattctacc atatatgcaa agttttttcg tactcttgat ttatgcattg accaaaaaaa
    14761 gaaaattagg caaaatagac aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga
    14821 atataaatct atgctgacaa agcaagagta cgagatattc atcgctcact atcaattaac
    14881 agataaagat ttccgtgtac agactaatat ttatttcgat accgttgatg aacagctgaa
    14941 gaaacaaaat tgtggcctac ggatccgttt cgttggtcat caagctgaat atacattaaa
    15001 aacacctgct gaaaaagggc gtctcgaaac aacggatacc tttataacag aagaagcaga
    15061 agcatttatc ttcgaaaaac gtctgcctcg ttcaggcgca gtttttcaaa aactttctga
    15121 tttgaatatc gatcctgctt cattaatcca aaccggaaag cttacaacaa aacgggctga
    15181 attccctatt gaagaaggac ttttagctat tgatgaaagc tggggagaca atctacacga
    15241 ttttgaactg gaactagagg taggggatgc atctgtggga aaaattgctt ttataaattt
    15301 tttaaaacgt ttttctttac cttatcgtcc tgctaagaac aagatccaac gtatgcttga
    15361 agctaaaaat tagaaacctt tcattttcac aaggcacttg agttcatttt tttacttttt
    15421 tctgttaagt tataggatgt gaaagaatct agtcactagt tgtatagaat ctattttggg
    15481 ggagattcat atgatcgaaa tctatttatt tgttaatcct ttaggggaat actgtttcga
    15541 aatggaacaa cagttattac aatttatcga agaagagtac gggacaccat caaaagaaaa
    15601 gatgcaattt cgctttcttc ctttggtcaa tttgcagaca attggggatg tcatgcaaca
    15661 aaaaggaatt tctcagaaca atcttgagga acgtaatcac ttattctcaa tgacttattc
    15721 tgcagcattg gactgcaaag cggcgcagtt tcaaggaaag aaaaaaggcc gccgtttttt
    15781 gatcaaattg caagaagcag tcggctgtaa tgacatacca tattctcaag aacttgctga
    15841 atccatattt gaagaaatcg gcggcgattt gaatatgttt aaagaagatc gtcagtctga
    15901 cttcgttaaa gaagtattct tttctgatca aaaaattgct cgggaaatgg gcatcacaaa
    15961 acacccttct gcagtcgttt acaattatgc ctgcgaccgt gattttggtg ttttagtaga
    16021 agtaaaaggt acaagtagcc tttggagaat cacagagctg tgtaaaacaa cagataactc
    16081 atatcaagtt tttaatggtg aacatttaca tacaaatgat tctcagttcc attcaagaac
    16141 tagccattta cacttgcttt ctaattaaaa aaatacgtcg actcccttac aagagagccg
    16201 acgtattttt ttcgtttcta ttcgagaaaa tcaaaaatag aaagccttat aaacgatcga
    16261 tcaattcttc taattcattt agacgttgtt caaacatcga cattgcatct tcaatatatg
    16321 cagcctgtgt catatcaact cctgcttttt tcattacttc cacaggataa tcgctgttac
    16381 ctgcttttag gtaagccaag tagttctcta gtgcctctgg ttcctggttt agtattttct
    16441 tcgccagtgc tgaagcagca gaaaagccag tcgagtattg gaaaacatag taattataat
    16501 aaaaatgcgg tatccgtgac cattcgaatt taatttctgg gtcttcttcg actgctggac
    16561 cataatattt tgcattcaat ttaccataac tatcacttag gtattcacta gtcaatggca
    16621 cacctttttc atcttccgta tgcatgaaat gctcgaattc agcgaattgt gtctggcgga
    16681 aaactgttcc tttaaaccca tccaagtaat ggttgagtac ataagcccgt acacgagggt
    16741 ctttttctgt ttccaataaa tactccgtta ggatattttc attcgttgtc gaagcaattt
    16801 ctgctaaaaa gatggagtag tcgccgtaca cataaggctg gttcgaacga gtgaaataac
    16861 tatgaacact atgtcccatt tcgtggacaa gcgtaaatag ctgatccagc gtatcatgcc
    16921 aattcaataa aatatatgga tttgtgtcat agcttcccga agaataagca ccgcttcgtt
    16981 tccctttatt ttcgacaaca tcgatccaac gttcagagaa tgctttttct acgatggcca
    17041 tgtattcttc acccattggt ttcagtgctt ctaaagcttt ttcttttgct tcttcgtacg
    17101 taaaggtaat tggagcttcc cctaagaccg gtgtataaag atcatacatg tgcagttttt
    17161 ccacttctaa taaacgtttc cgtaattcca tgtatcgatg taacaaaggc aaatgcttgt
    17221 ttaccacgtc taccaaagta tcgtatacac tttcaggaat atgattattg ctcaaagacg
    17281 cttctctggc agagctgtaa ttacggactt tcgctttaaa attatgtcct tttatatgtg
    17341 tgcctaaagt agaagcaaat gtatttctaa attgttcgta aacactgtac aatcccttaa
    17401 atgctgcttc gcgcaccctt cgatccgtgc tttctagcaa ctgaccatac acgccatgag
    17461 ataattggac tatttcacca ttttctcctt caatcgttgg aaaaactaga tctgcattat
    17521 tcaaaacagc aaatgtatca cttgaagcat caaagatttc acctgctcca gcaagaagag
    17581 attcctgctc agcagataag acatgggctc gattatctac tatttgctgg atataatggc
    17641 gatagacttc caattttggt tcttctttga aatattgcca aatctggtca tctgacaatt
    17701 gcaatatttc tggttcaaac caggaaacgg cttcactaac tttcgaaaac agactgcttg
    17761 ctcttgcata aagcgcttgg taatctgtat ttccagtgtc ttgatcgttt ttaaggtgcg
    17821 catatacata aatgacttca gtttggcgat aaaccctcaa tacgaattca atcgcattta
    17881 aaaattgaga agcgccttga tcaagtgttc ctttgtgttt ttcagattgt tttaactctt
    17941 ctgataattc caaatatttc tcatcaaact cttggtcgct tgaaaagatc ttggtcaagt
    18001 cccaagttaa attttcaggc aattcttctc gttttggcaa ctgctttact tccatgaaat
    18061 ccactccttt tcttccctta gctctatcta tcaaaataga tcatagtcta acatccacat
    18121 gtcacagcat ctaacttttc tactcattca tgtgtctgct tccatcaatt ttagcataac
    18181 taaatcttgt tttacataga aaaccgattc ctagcctatt tttatactta ctattttttc
    18241 aaagaagaca agtgtatgca ttcgttaaaa aactgctgat agaccatctc ttcatcaatc
    18301 ataggaaaaa gccattccct tttatgttct tttatttggc aaagccatga gttgaaatca
    18361 ggaactttct ggttttccaa ggcttcttcg tacaacattc gataaagaaa aacctgttct
    18421 tgtaaataaa agaaaaaccg acttttttga tagatccacg atgatagatg caaaagatgt
    18481 ttatgacgca agtagcattg ttcttgtatg gatactgtat tcttttgctt gtgaataagt
    18541 tggcgtgtca gccatttctt cccatcttca ctcacttttt ctattttttt catttggttc
    18601 attttaattt cgttactagt gaatatttct ttaaggtccc tactagaaaa agtccagcag
    18661 ctactgcaaa aggaaatatg ccctgataca gtctctgtaa tatgatgata caaataaagc
    18721 ttttgtttct tgagatcagc tttccagcaa tgcactcctc tttttctatt atagtagcag
    18781 caactttttt ctatcaatga gaattgcgat tgtccaagaa aagaattacc aagtatccac
    18841 catacttggt aactgttatt caaataattt tgggttcttt caataaatct ctggtgagac
    18901 agtctagaac attggatttc tattgctaga ttcgccaaca aaatatccgg cctttgccgc
    18961 aacctcggca aataggcttc caaaaatact ggttctactg actgtcccag ccaatccatg
    19021 aagcattcct ttaactgtaa gtgctctttt gattctcttt cactaaagac cgaacagtta
    19081 tctgattttt gatgggcaaa atgagcacat tttttctttc cctttttcaa aatcactctt
    19141 tttcgacagc cacgacaata atatttcttt cctttttctg catgtttagc aaaacagcac
    19201 ttattatatt cattcattgc aagcagcatt tttctccctc tgaacttatt tacgtctaag
    19261 aatttattta cacgaaaaaa gagggcgtga aaaagctatt ctgcatcaag taataagaac
    19321 agctaaacaa aaatagttcc caatattttt cgtttggttg ggcttgttac cgcagatgca
    19381 agcttttgaa cacatttata cgaaaaaaag actgcgacac aatttttgtc acagtctctt
    19441 actataatgc tgatttatac aaaataaaat ttcgttcgct cgatagcatt acgcgccatc
    19501 agacattgtc catattcatt caatgtttct ggtgtacgat cgctcttttc agcaaactcc
    19561 aaaattcgag caatctgatt ctctacatcc gcttggttga gattttccat ccagaagtga
    19621 acgactaggt aatagcgctc ttcgaataaa tacaaatcag tccaagcaga ctctagttcg
    19681 acatctgttg caagttggat cacttgttca aaatcttcaa aagcaaaagt tgcttgtctt
    19741 tcctgttctt ctagggcgct tccagataat tcttcctccg gatcgctgcc aataatctgc
    19801 tgacgaagaa agtcactcac atcttctgaa tcaacagtag aatcttctga ttgagaaatt
    19861 tcttcatccg gaagattttt actgataaac agttctaatc cgtctccttt tggcaaaact
    19921 tggaaagtga cagcttcgct tcctcggaac tcctcttcga tatcaacttc ttccaaaata
    19981 ctataaaaga aattttcgat ttctttgtga ttgccaagta aatccaaaaa tgaaacgccc
    20041 cgttcctcta aatcttcatg accaattagt acacggatcg tattttcatt aatatgttcc
    20101 atttccattt tgctacacct cactttgcca caatgtttaa tatagattca ttgtagcgaa
    20161 atcagcttaa atgtaaagaa aaaaaatctc gctgttaagc gaatttaata aaaccagcgg
    20221 atttttcaac ctttcaagct atcttattta actataacat ttttggcaga cgaatactat
    20281 atagccgact tttcagctaa ttttccctct ctatttttcg tgcaatcatc atcttctact
    20341 acctttttat acatctaatc aagaatactt tttgtaaaaa caaaaaaact tggaaaaact
    20401 atctttgtaa tagtcttt
//
LOCUS       DAJSBM010000050        20314 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJSBM010000050 DAJSBM010000000
VERSION     DAJSBM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.050"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(284..781)
                     /locus_tag="ONT67_001975"
     CDS             complement(284..781)
                     /locus_tag="ONT67_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCR2355707.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(911..1621)
                     /gene="budA"
                     /locus_tag="ONT67_001976"
     CDS             complement(911..1621)
                     /gene="budA"
                     /locus_tag="ONT67_001976"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCR2355708.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1634..3298)
                     /gene="alsS"
                     /locus_tag="ONT67_001977"
     CDS             complement(1634..3298)
                     /gene="alsS"
                     /locus_tag="ONT67_001977"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCR2355709.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3506..4168)
                     /locus_tag="ONT67_001978"
     CDS             complement(3506..4168)
                     /locus_tag="ONT67_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCR2355710.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4178..4993)
                     /locus_tag="ONT67_001979"
     CDS             complement(4178..4993)
                     /locus_tag="ONT67_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355711.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5254..5697)
                     /locus_tag="ONT67_001980"
     CDS             complement(5254..5697)
                     /locus_tag="ONT67_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCR2355712.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5831..6169)
                     /locus_tag="ONT67_001981"
                     /pseudo
     CDS             complement(5831..6169)
                     /locus_tag="ONT67_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6157..6534)
                     /locus_tag="ONT67_001982"
     CDS             complement(6157..6534)
                     /locus_tag="ONT67_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCR2355713.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6758..7951
                     /locus_tag="ONT67_001983"
     CDS             6758..7951
                     /locus_tag="ONT67_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355714.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8117..8539)
                     /locus_tag="ONT67_001984"
     CDS             complement(8117..8539)
                     /locus_tag="ONT67_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355715.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9122..9616
                     /locus_tag="ONT67_001985"
     CDS             9122..9616
                     /locus_tag="ONT67_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355716.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9778..10950)
                     /locus_tag="ONT67_001986"
     CDS             complement(9778..10950)
                     /locus_tag="ONT67_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2355717.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(11156..11971)
                     /locus_tag="ONT67_001987"
     CDS             complement(11156..11971)
                     /locus_tag="ONT67_001987"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2355718.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12121..12567)
                     /locus_tag="ONT67_001988"
     CDS             complement(12121..12567)
                     /locus_tag="ONT67_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2355719.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12564..13568)
                     /locus_tag="ONT67_001989"
     CDS             complement(12564..13568)
                     /locus_tag="ONT67_001989"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCR2355720.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13608..13937)
                     /locus_tag="ONT67_001990"
     CDS             complement(13608..13937)
                     /locus_tag="ONT67_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HCR2355721.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(14063..16390)
                     /locus_tag="ONT67_001991"
     CDS             complement(14063..16390)
                     /locus_tag="ONT67_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2355722.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(17233..17526)
                     /locus_tag="ONT67_001992"
     CDS             complement(17233..17526)
                     /locus_tag="ONT67_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCR2355723.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(17929..19107)
                     /locus_tag="ONT67_001993"
     CDS             complement(17929..19107)
                     /locus_tag="ONT67_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2355724.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(19211..19525)
                     /locus_tag="ONT67_001994"
     CDS             complement(19211..19525)
                     /locus_tag="ONT67_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355725.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            19632..19850
                     /locus_tag="ONT67_001995"
     CDS             19632..19850
                     /locus_tag="ONT67_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355726.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(19845..20240)
                     /locus_tag="ONT67_001996"
                     /pseudo
     CDS             complement(19845..20240)
                     /locus_tag="ONT67_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         5942 a         4005 c         3466 g         6901 t
ORIGIN      
        1 attttctaat ttactgatta cttctccaac cactttttgt ttcaagccaa taggttggca
       61 aatatatttt tttcctaaaa tgatagtttc gttattcata tttcatccat ccttaatgtt
      121 ttttcattat tcaaaatgat aataccatta aaatatgatt gtgaaaatca ataacacttg
      181 tgattttata aaaaaataaa aaaaccgaaa aacagaaatg accactgtct ttccggctct
      241 ttttattctt gatttctctt cttttttgtt tcaatagacg attttagtta ttaataaatg
      301 aaaaagcatt tatattaaaa tttgctaata aaagatctac atatttttct agaaatgctt
      361 gatcgatttc atcataccct ttcgttactc cgcaatcgat atctaaaaca ccgattaaat
      421 taccttcttt taccattggc actacgattt ctgattgtgc ggctgaatcg caggaaatgt
      481 aatttgcatg ggttttgaca tcatcaacga tcaatgtttc ttgtttttca gctgattctc
      541 cacaaactcc ttttcccatc ttgatacgtg tgcaagaaac tcgcccttgg aacggtccta
      601 aaatcaattc attaccatcg aataaataat atcctgcaaa gacagtctca ggcaaagtct
      661 cattcaacaa tgcagatgaa tttgctaaat ttgctaacca gttatcttct gcttctaata
      721 atccttgttg ctgtaataat aaaatttcgt atgcatcttt tttctgtttt tctgtccaca
      781 tgataaaaac cgctcctttt tgatctatgg agaagattat agctcagttt ttttgtaaac
      841 gctaggtttc ctcctcaaaa aaacgagata cgtcaggtta atcaccttga acgcatctcg
      901 ctgtcttttt ctattctgct tgttggattt ctgctgctaa atcagcataa tcaatcgttt
      961 cgtgaacaaa agtcggatca tcaactggaa aatgctgtac aagggacgag atgcggctaa
     1021 tctcaacttt tccttcagcc acttcaaaat ccattacatg tccgccaaat gtatgtgtat
     1081 catcgataaa atgcaggtga aatcccgctg cagcaactcc ttggaataat tcaggcgtaa
     1141 agaacccgac taatgttcct tggatattgc tttgttggaa ttctggttga gactctgaaa
     1201 tttttgctag tcttctgtaa ggtttttctt gcttcggcat gatacggatg tgcatatttt
     1261 tgaataatcc agttatcttc actgccaaaa ataggttttc cccggcttct cttactaata
     1321 tgtctttttt caattgttct ccatctgcag tttttgagac agaaaaacta gtatctggcg
     1381 taaaatctgt aattgctgca tagggggtaa gttcacttcc atctaattgg gcaactgagc
     1441 catctgatct tccttgataa gctacgccat ctaaaaagat gacttcacca tcaagaccat
     1501 gcaatgttcc caatcccaga gttccttttt ctaatatatg agcaatcgtt tctgttccat
     1561 ccatcaagcc ggccatcaaa gctcctaatg tcccgtgctg gtataagatt ttttcagtca
     1621 tgtcgacact tccttaatag aattgatctg gtaagattgt ttctcccaat ttctcattat
     1681 cacggtaatc aatcggaata tcaataatta cgggtccttc tgtcgcaaat ccttcttcta
     1741 aagctttttc taattcctct acacttgtag ctcggattcc tttagcgcca aaagcttctg
     1801 catatttcac aaaatcaact ggaccaaagt ctacacctga cgcccgctga tatttcattt
     1861 tttcttggaa ctctaccata ttgtagtgtc catcattcca aatcaaatga acgatattca
     1921 attttttacg taccgccgtt tctaaatcct gggcagagaa taaaaatccc ccatcgccgg
     1981 aaactgatac gatttgtgta tttggacgca ccaatgcagc agaaatcgcc catggcaatg
     2041 caacaccaag cgtttgcata ccattactga ataacaaatg acgaggttca taacttctaa
     2101 agtggcgtgc catccaaata taatgactac ctacatctac ggtaacagtc atatcatctg
     2161 ttacttttga ctgaagcgtg ttaataactt ctaaaggatg caagatacct gcttctcctt
     2221 ttgattgaac aatgtcccgc tccgttaatt ttgcttgaag agatgctaaa tattcttttg
     2281 catcttctga cacttgttga ggctctaatg aacctgtcaa taaatcaaga gtggctgaga
     2341 tatcaccgat caattctctt tcaggctgca taaaaggatc gatttcagct ggcgcttcat
     2401 cgatcacaat gatccgcgca tctttttccg cattccagtt tcttgcttca tattcgattg
     2461 gatcatagcc aattgcaata actagatcac ttcgtttcaa aagcatatct cctggttggt
     2521 ttctaaataa acccacacga ccaaagaaat gcgcttctaa ttcacgtgag atcactccag
     2581 ctgcttggaa tgtctctaca acaggcaatg ctgtcttttc aactaattgc ctgatggcta
     2641 acgtttcttt ttcacttgat cctcgcatac ctactaatag taccggtagt tttgcttctc
     2701 taatgcgttc agctaggtaa cggatatcat ctgcagaagc agaacctaat ttaggatcgc
     2761 tcaatggctt gatgacatta ccttggacag gtgcatccac tacatcttgc gggatactga
     2821 taaagctcgc gccttttttt gaactcttag ccatacgata agcattggca atcacttctg
     2881 aaattgtttc tggatcttgg atttctgcac tgtattttgt gattggctgg aacagtgctg
     2941 cattatccat gctttgatgg gtcaacttca atagatcgct tcgttttact tgtccggcaa
     3001 ttgctaaaac tgggtcacct tctgctgtcg ctgttaccaa acctgtagct aagttactag
     3061 caccaggacc acttgttgcg ataactactc ctggctctcc tgtaatacgt ccgattgctt
     3121 gtgccataaa tgcagcattt tgttcgtgac gagtcacgat taattcagga ccttgatcct
     3181 ctaattcatt aaatacacca tcgatttttg cacctggtat accgaataca taatcaacat
     3241 gatgattaat caaactttct actacagatt tcgaaccttg tttcattttt tcagccattg
     3301 aacatcgtcc tttttatgct ttatttttaa ttattagtta gaaataatca caaataagtg
     3361 atacagtttc taaacctgtt tcatacaaaa gtatagcacg ttttatttca tttgtttagc
     3421 catatgtttc aaaaaatgaa aaagaatgac agtttttaca taaaaaaaac tgtcattctt
     3481 aattgaaagt ttcaatttat aaagtctaca gatccatagt tctcatcaat ttacgattgt
     3541 tgaagtaaat caaaccaaat acgatagaag gacctagtag aatacaccat tggggcttta
     3601 cgaaaaagaa gagaaacaaa cttgtcaata gtaaaaaatc tagtaaactc acataccatt
     3661 ttttcaacgt atagtagaac gctatccgta gaacttgatt tggtttcgac gctggatttc
     3721 tcacctgaaa ataccagcag ttggtgctaa aggaaactga taaacaagca agtaaaacaa
     3781 acaacggaat caaccatttt ccataagaaa aacgtataaa aaagaaacaa tcaaaaatca
     3841 ttatcacact aactatccaa gcaaatagcc aataaaaaaa tccctttagc caaaattggc
     3901 gaaacatctt tatgaacaaa cgtactggtg aaaaactatt ttcttttgct aattcttctg
     3961 tcatacctat catcacgatg caacctggac caataaaaag taaagaaaaa ataaaggaaa
     4021 agacattcct tccatctatc gacaaaagga acgcagctgc tatatacaag atattgacac
     4081 accaaaaaca aaggctactt gccaatgatt gatacaacca ataaaagaat tccatataaa
     4141 catttttgtc aaaacgctgc tctttcataa tcacctccta ttccttcatt cctgatgaag
     4201 cgatcccttc tacaaaatat ttttgcatcg ctaaaaatat aaaagcaaca ggaagaacag
     4261 aaacaactga ggcagccatg attaatgcgt actctgcatc aaatagccca ataaacattt
     4321 tcaaccctaa ctggattgtt ttattttccg tagaagacaa ataaatgaat ggccccatat
     4381 aatcgttcca tgtattaacg aatgtgatga tcgtcaaact ggcaatcgcc ggttttgtaa
     4441 gaggtaaaat gatcttgcga tagattcccc actctgataa gccatcgatc cgcgcactct
     4501 cacataaaga atctgggata ctactgtaat attgcttcaa caagaacaca ccaaaagcat
     4561 tgaacgcttg taatagcacc aacgaccaca acgtatctgt taaacccact tttctcatca
     4621 taatgaactg cgggatcata taagactgcc aagggacagc aatcgtgccg atatatgcta
     4681 aaaataaaat atcgcgccct ttaaaattta gtttagaaaa cccgtaagca gcaaaactag
     4741 aagtaaacaa ctgaatcaac gtgatcacaa cgcttaaaaa agctgtattc ttaaaaaatg
     4801 tcactaaagg aatacgagac caaataacca gataattctc taaatgccaa gttttaggga
     4861 tccattgaat cggaatcgtg aacacttcat tatttgcttt gaatgaagca gaaaccatcc
     4921 ataaaaaagg aatgatcgtc aataaagcta atacaatcag cagaatcata cccaaccatt
     4981 gaataaaggg catgtttttt gaacgaattt ttgctttttt tctttcattt cgaacacctc
     5041 caaagttata atcttcattt ctttgtaatg attacaatcc agaacaacgg ttattatagc
     5101 tgattcattt ccgttcttct tatttttgta agcgcttatt taacaagtta tgtaaatctc
     5161 tttatttttt tgatttaaaa atgaaaaaat acaaaagcaa gactgtcata cgtctcgctt
     5221 ttgtattgaa atttcttcga ttagagatac ttattactgt tcaaaagctt ctttgaaacg
     5281 gtgaatcaat tcattgacta caggtgataa aatccgattc tttttccaga ctagcatgca
     5341 gttcgtttca atcattgggt caagcggaac gaatgtcact cgctttgaat gatgacttac
     5401 agcaccttct atgactaaag cagcacctac ctgattctca attagcgcaa atacattgaa
     5461 aatcaaattg tacgttcctg tgatcagcaa ctgctccttt tttatgccta accagtcagc
     5521 aaacattcgc tgtatttctg gacgctttga gatcaaaaga ggtacattcg caaagtcttc
     5581 ttttgttatc acgtcttttt ttgctaaaac agaattttct aaaatgagca atccccattt
     5641 ttcctttctt ggtaacaaaa tcgtttcgta tttctctgta tctactggat ctagcaaaat
     5701 cgctaaatct aacaggcttt tttctagtca ctccgtgata tcatcacttg ttccgctgtg
     5761 gatatgaaat tgaacgctgg gataatcacg gatcaacttt tctagtaata gtgccaatgt
     5821 attcgaacta tcagcttcca cacagccgat cgtaaatcgg ccgctgaata attgtttttt
     5881 tctgttctcg aattcttgag tggtttgatt cgttaaatct aaaatttctt ctgctcggct
     5941 tttttagaaa ataacctgct tctgtcaaga tcattttgcg gttttcgcgt acaaacagtt
     6001 ctgttcctag ttactcttct aactccttca gttgtcggct taaggttggt tgtgtaagat
     6061 gtagaacttc tgcggcttga gagattgttc cttcttctgc aattgtccaa aaatatttta
     6121 gtaagcgtaa ctccatagcg atgctccttg atttttttac ttgatacatt ccattttatt
     6181 tctagataac acacgatcat atttcttgat ttttccttgt aaacgttcca aacattcttg
     6241 catttcagca atttttttcg tcaaaagttc actttcttca atcaaaagct tacgccgctc
     6301 aaatacagtt gcttctcctt cgctagatag gcgaatatat tcaatcaaag actcgatcga
     6361 taatccggca tttcgcatac agatcgtaaa gtcgacccat tttaggtctt cttctgtata
     6421 atcacggatt ccgccttttg tccgattaac tggtggaatc aacccgattc gttcataata
     6481 gcgcaatgta tcggccgaaa tgcccttttc ttcactgact tgtttgatat tcatctgatc
     6541 actctttctc ttaatttctt gccactttac ttatgtattt tatttattct acaactttaa
     6601 gtgtactcca agtcaatact catttctatt aaaactaagc tggattatct atatcggttg
     6661 tttgtgtatt atttcaaata aaaaagtctt gtttttttat tcttttttgg atatactttt
     6721 aacaatttag ggaaatttta ggaaaagggg tataaaaatg acaacgtatc agcgaaaggt
     6781 attagtatcg acatcggcgg gaattgcttt agagaatatg gatattatgt ttttagcttt
     6841 ttcattatct tcaatgatta cttcttttca tattagtggt acgcaagcgg gtttcatttc
     6901 aacgatcact aatttgggga tgctcattgg aggtattttt tttggcatca tagctgataa
     6961 atatggacgt gtgaaagtat tttctcaaac ggtccttctg ttttcgattg cttctttatt
     7021 gatgtatttt gcttcaaaca tctacttggt ttatctcttt cgctttatcg caggtgtagg
     7081 agcgggagga gaatatgggg cgtgtatgtc cttgatttcc gaaagttttt caaaaaaaca
     7141 aattggacga gcttcttcgg ttgctggtat tggtgcgcag gtcggtgcgg ctttggctgc
     7201 gattttagct gctgtgatca ttccaacgtt tggttggaag atgttgtatg tcgtaggtgt
     7261 actgccggtg ttgatggtct tgttcattcg tcgcggcttg actgaaccaa aggaattcaa
     7321 atctgtacaa tctgccgaaa agaaaaccaa acttagcaga ctgtttgaat caaaacatct
     7381 tgcttggcaa acatgtggcc ttagtatcat ggtaactgtc caaattgccg gctattttgg
     7441 gttaatgaat tggctacctt caattatgca aaatcgatta gggctttcgg tttctgggtc
     7501 ttcattgtgg atggtcagta ctattttagg catgtctttg gggatgatga cttttggcat
     7561 aatcatggat aaattggggc ctaaactagc cttttctttg tttttaattt gttcatcctt
     7621 atctgtctat ctacttgtgt tagcccatag ccagtggagt ttggtagcag ccgctgtgat
     7681 agtcggctac tttatcaatg gcatgtatgg tggatatggc gcagtgatta gtagtttgta
     7741 tccaacagaa atccgcgcga cggcgaataa tttcgttatg aaccttggac gagctgttgg
     7801 tggtttttct tctatcgtca tcggtttctt gatggatcat tatagtttaa ctgccgtaat
     7861 cttgtttttg agcggtattt acctagtcag tctgctggtc ttgacaactc ttactggtgt
     7921 tacagcatta agggaaagct tgcagcattg atagttgagt ctgtaaatga caagaaagag
     7981 gtgtggaaaa agcttgcatc tgcggtaata agaccagcca aatcaaaaac atgagacact
     8041 gtttccgttt ggctggtcgt attacttgat gcagggcagc tttttcaact gctaatttcc
     8101 ttctatctgg cggcgactat tctttgacgt ccgtcatttc aaagtacttt actaataact
     8161 tagcataacg aaaaactgtg gatacactcg tatattgagc gtctgcataa tcttccaaat
     8221 ttttctcagg atgttgatac atgaattcaa tcaaatgata attatatgat tttctgtaga
     8281 aatgaagata aataatgtag attgggaagt tctcctgctt ttccatagaa aagcgccgat
     8341 tattctcatt cttcaaaatg accttatcag gatattgttg gaacaattct tctaaatctg
     8401 ttagataatt caagatagac ttacttgata ccttcaattc gttttgtagt tcttctattg
     8461 aacaccaacg ttctgtttca agcagtaaat gaatcatttt aattttcttt tgagaaactt
     8521 ttgttaaaaa agtctgcata ttttctcctc ctgtatctac tttactatac tacatacaag
     8581 aaaaaaacgg atacaaatat catattgtca caccgtcaat agtgcataaa cctgttatta
     8641 tttctctttt tttcctaagt attctattga tatgaatcat ttaatcgttt attatataaa
     8701 tgtcaataat ctaataataa aataacgtac atattttatt attgcatact taataacaga
     8761 actatagcaa aaaatcccgt gtatcaacac gggattagat taagtttcct caatcactat
     8821 tcataacaca attattataa caagtaagta aacaattgaa aaggacattt attgaaaacg
     8881 gttgtttttt acttaaggag gaaatttagt gaaaaaatta gttgcgttgt ttttggtcgc
     8941 aggaagtttg ttgagtgggt gtggtgctac tacgtctact actcaaagca caagttcagc
     9001 tcaggcttcc agcagcagta ccacgatctc cgaaagtacg gcagattcat cgaaagaaca
     9061 aattgaaaag aaaaaagaag aagagaaaca aaaagcggaa aaagctgcac tagaaaaaaa
     9121 agtgacagaa gcggaatctg ctttgtcaca agctgaagca aatcctacaa aagaaaacta
     9181 tgcaactgct tcagcagcta tccaagcgct acctgaaaac aaacaagagt tgacaagccg
     9241 tcttgctgca attgattcca caatcaaagc aaaagaagca gaagaagaac ggaaaaagca
     9301 agaagaaatt gcacgacaac aagcagaaca agcgcaacaa gaagcaatcg cacagcaaca
     9361 agcagaacag gcgaggcaag aagcaatcgc acagcaacaa gcagaacagg cgcgacagca
     9421 agcagcagct gaacaggctt ctcagcaggc acaagccgca gctcctgagc aaaataatga
     9481 acaaactgtc tatgtcacac cgaccggttc aaaataccat acacataaat gtggaaatgg
     9541 tacgtatacc cctgccactt taagcgaagc tcaagctcgt ggtttaaccc cttgctccaa
     9601 atgttatgga aactaattca aaagttatac tataataata aaattcttct gaaacataaa
     9661 aaaatagaag ctggcgttcc agatagtaaa ccaaaaatag cagtttacac gtccatttcg
     9721 tcagcttctc tcttttttca tttagtctgt tccctctgct tttttggaca tcttcattca
     9781 ttctttctca atcaaatagt acacctcttt ttcttttcgc agtttatatc ttcctttttt
     9841 caatttcatt gaaaactgtt tcgaatgttt atgttttaca tggattttaa gcaggagctc
     9901 ctcatgttgc aagcgatagt tcctgccttt taaatcagaa aatctcacta gtccgtattc
     9961 atcagaagct ttcacaaatg gaactttttc tctagtaata tagtgtttgc cgttgagcgt
    10021 ttttacagaa aagaccattc cttcgattgg tcgtccgttt gtgttcttta cataaaaaga
    10081 taaaggatag tttcgcttag cgatggccaa agaatctaaa taagcgatga gtagccaaac
    10141 taagccgatt gatccaagta gtataccgat aaaccaaata aaaaatttcc attttttcca
    10201 ttgatcaatt ccttggatcg ctttttttgc ctcttttgct tgataaggaa tacgatgccc
    10261 cgtcacaagc agtcgatgtg tattgaccat ataaggagtg caggtaagca aagtcacgag
    10321 atctctgcct ttttggatac ccaaagaatc tacttcgtca ggtaagacca cttggatttt
    10381 ttctacttga tacgccaatg tctttccatt gatctggatg aaaaactggt ctcctttttt
    10441 caacttaggc aaatctgtaa ataattttgc ttctgggagt ccacgatgcc cagaaagaac
    10501 ggcatgggtg tttttcccgc cagtcggata agaacttcct tccaataatg acgttccttt
    10561 ttgcaaaaat atttccgttg tctgatcgaa tataggtaag ctgacattga tttttgggat
    10621 ttcgatgact ccaagcatat gttccataaa aaaggcatta ctaggtaaat cttttaatgc
    10681 ttttgcatca actgcttggt tgaaactgcc tatccctggt gatacgtttt gctctgccaa
    10741 ctgttggttt ttctttgtca ttttttcctg ctgttttttt atttcctctg aattcttatt
    10801 cgatgcttgc ttttgataat ggatgatcat ttgctgggca agaaaatcat tcagtgaatc
    10861 ttctacaaaa ggataagaaa agatcccgat cccaacgact aaaatgattg ccatgaagat
    10921 gtccagtatt cttattctgc ttttattcat catgtccacc tattttctaa tatgtatatg
    10981 aagaggttgt gaaatcagcg ccttgacctg agtattagcg aagcaaacgg caaaatagtt
    11041 cctcctattt tgccgtttgt tgagcgaata ccaagagacc gaccacttgt cacaaccttt
    11101 ctccccattt gaatcactat cgtttattta tcgtatgatt tttgtttcca atagtttaag
    11161 cttctgtttc ttgatggcgt tttttgaaat agatcactgc tcctccaact aaagcagtac
    11221 ctaccaagat gaatacgatg atccccatac caccagttga aggcaagatt cctttaggtt
    11281 ggttatcgat cgttgtttca tcagaagttg ttggatcatc atctactgta aattcagtgt
    11341 acgcagcttc attcttaggt aatgcgtagc cttctggtgc ttttgtttct tgaaggcagt
    11401 atgccccttg tttcagccct tcgattgaaa atttcccttc tgaatcagaa atcaatttca
    11461 cttcttccca gtcagaacct gtgacttcat ctgaccaagc atatgggtat tctcctgagg
    11521 caaggtttgc tatttttttc attaatttaa cagttggatc ttttgctctt agaatagaaa
    11581 attctgcatc ggctaatttt tcttgcgtgt tcacatcttg tttgatgaag tggaaatcac
    11641 cgtagttttg gacattttct acagtaatcg tgttatccgt agttgtattt tcccgattga
    11701 ctacaccgtt tggattccca agattggctt catctgcgcc atggatgata ccggttgttt
    11761 gttgcaattt tccgtttttt actataaaag tagtttcttt ttgtggattg gttaattgat
    11821 aaccatctgg tgcttttgtt tcaactaaag tataattgcc gtctattaat ccattgacac
    11881 tgatggatcc ttcagtatca gttgtgaatg tcaacgctga ctcagcagga tcatagagag
    11941 cgacaccagt gttttcatca aaaccggtca cgaattgatt ctctgaattt ttgatctgga
    12001 attgcgcccc tgctaactta tctacccctg aaaagttccc agctttctgg attttgatat
    12061 tgctttcttt gatgacattt ttagcatata ggtgaatcgt gtctaaaggt ttgtcagggt
    12121 ctagggtaag cttaacacga tatttggcga cgtttcgaca ccgggctttg gtgtttctac
    12181 aaagacgtat acagcatctt tcccatcaga tttacctgga agagtcaggt tagctaatcc
    12241 ttcaccagaa gtcgttacac ttgcgaaggg tgtagcgtct gctggaactg ctgtgtcttt
    12301 acttggatag tacgtagcag tgatgtcata tacgttgaat tcaacgccgt tcaacggttc
    12361 gctattcgga aatgtcaatt ccgttccgtt gtttatttga ttttctggtg ctgaagtgaa
    12421 tttcaattta tgcaaattaa ccgtaacatt ttctggtgta ggtgatacgg ctgcagaaac
    12481 ctctgcagtt cctaggccga gaacgaaagg aagcagcatg atagcagttc ctaaaaacgt
    12541 ccatattttt gttttgatgt ttttcatttt atttccctcc tattgtttcc tttttgttgt
    12601 tttttataaa accaagctgt ccctgttgaa aggactagca ataaccctaa aattgagaaa
    12661 cctccaagca tctcttcgct tgtttttgga aagcttcctc catttttttc atttgatgga
    12721 ccatttgtct gcatctgaac tggcgttttt ccattattcg tcggatttgc ttttggttcg
    12781 ttgatgattt ccaatggtat aggatgaagc ggatcataac tgcctttttc aacttcaaag
    12841 agaatggttg attgactcaa tatatatcct ttaggcggtt taatctccac tagttcgtaa
    12901 cttccaaaag acaatccatg gacagaaaat gttccatctt tactggaagt aaaaatcgtt
    12961 gctcttgaat catctttggc tacccaatga tttatcccgt tttcttgcgc taagtattgg
    13021 tcattttttt tgactagaaa ttgagcatct gctagttttt gtttcgtttg ccgatccact
    13081 ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg tttctactgt ttgattgtca
    13141 gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac tgttttcttt caatgtcatt
    13201 tggtaacgga tcgttaattt ttttccagca aacgaactaa gatcttttcc agaatctgat
    13261 aagacaaaac catgttttat tttttgtgtg tgaaatgtat gaatcttgtc tccaccaatc
    13321 aaaaaatcga tcgtttctgg ttctagccat aactgaggat cagctgtatc tttgatcgta
    13381 taggttccta tctcgttgat gttctcagga attgttacgg tgatgagata agggacttta
    13441 tccccatctg caaaatctgt ctgcttattc tctactgttt ttgttaatgt gagtgcagca
    13501 ggtatttttt gttcgctctt tggatacaag tggatggtcg tcaatttatg attagatgaa
    13561 tctgtcacag gaagcacaac gacaaatggt gctgattttt cttttactta atctgaagtc
    13621 tttatttccg taaatcggta aacagcgtca cgaccttgct catctttgtc cgatgcacta
    13681 aaagaagcaa tgccctcttc ctcgtctact gtttttgtca cattttctgc cagtattttt
    13741 tcactatcac ttttttgtgc tagttttctt tgtgcgtctt ccacactact tccttctgac
    13801 cgcagcttgt aaaattcatc cgagacatca tatatagcga aagtaacgcc gttcaaccct
    13861 tttactcctt gaaagttatc cgggatattt attccgtcgt tttgactgat acttggcatc
    13921 ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa taggtgattc tctagctgac
    13981 acagtcggag aacctataaa aaagaccagt aggataaaaa agagaacagc aaatatatcc
    14041 acacgtacat tttttgtttt tttcatcgct gttcacctgc ttttttctta cgatggataa
    14101 aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa gacaaagggc agtagaccta
    14161 tcccacctgt cgaaggaagc aagagagtcg gattgatcgt caacttagcg gtatctgttg
    14221 cgataatcgt ataattattg tttacacttc cttcgcctaa actaatcgtt actatttgtg
    14281 atccaatatc tgtccggctg ttaactgtta gggtataatc caatgattcg cctaagacaa
    14341 caccggtaac gtggagattt ccaaccgaaa tcgagtgact ttgggcatca taattaaagg
    14401 cagcgtcatc gacagtaatc actgcgttag ctttatagat ggtataagtt cctgatgtgt
    14461 tggctatatc agtaaatgac aacttgggat ttgctgcttt gactttgtct attccgctat
    14521 tcgttagttt aattgtatag ctgccagcat taaccatatt ttttgctgat tgtgcatctg
    14581 ctgtgtaatc catactgcta agtgccactt gtactggagt aagcgaacta gtgacaccct
    14641 ctgctagctt gacatggatt gacgggacac ctgcttttcc atcatatgtc ttactgccac
    14701 tgagctctac cgcaccgctg atttgcttaa tcgtatagac atcagatgtc tgcacgatag
    14761 taaataaata gtttgttctg gcagctagtt tagctagtcc gctttttgtc aattgccaag
    14821 acgtataggt tccaatttca gtaggcgcag ttacttcaac tcccttttca ttcatcgctt
    14881 tgaaatcagt tggattccat gtgatttgat caatactgag atcattgttt aaaagggatg
    14941 ttgattctgt tttatcatta aaggcatatt taacgatcgg tacaacagaa agaccatcat
    15001 agtacttact gccatttgca ttgattgata ctgttgcatt tgtcttataa tataggatga
    15061 cttcagaaat ctgttttcca tttgcgtcct ttacgattac atttccattt gtatctaaac
    15121 tagctaaaac actcattgct gattggttct ttgtttctat cgctgaaaca gctgtaaagg
    15181 tatatccgtt gatagtaggc gtattagtcg tcaggtcttg tgcagcactt ccgtaagtaa
    15241 gattcattat tttagaagga gcaatcatct gtccaaaata gtcttgatac ttaatagtga
    15301 ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt actttctagg gctgtgaaag
    15361 aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt tggagtgata accactttgt
    15421 cacccacatg gattccagat aatgtagtcc cgtacgttcc gatttttgct gctccaccat
    15481 tgactgtata agaaaaagtt cctgtccgat tcgcattgac ctgaaccgtt gctgcttgat
    15541 aggttggaaa atttgaaaca tttcccttac catcaacgat ctgcccattt ttgatcgagt
    15601 accccgtgaa tggaatatca tttgctaact gtataccgat tgaaaaagta ctcatacctc
    15661 ctccggcagg aagccctccc catgttgagg gtgtaaaaga ataactttta tccgcattgg
    15721 gtgtaactga atcgatcgta tttccagcgg tagccacccc catatagata ctttttggga
    15781 atgtgatagt agtaccattt actgtggagc caataaacag attatggata tctggacggt
    15841 tatcaataaa agcgattgct ttggttacgc cttgtaacgc agttttatcc gcactcatcg
    15901 atccgttaac cgcaacaatg actccatctt tggcatatat cactgtatat ccatctggta
    15961 tggtatgtcc gttgaaattc gcagtggccg caacagtact aaatgtctgg cttctacctg
    16021 tttctgaata agctttctcc cctgctgctt ttgctgctgt gatttctttt tctgtcggat
    16081 caatcagttg agtcgaggat gtggttgagg ttgtctcgtt gttgctagac tgattggtgc
    16141 tttctatgga ctgtatttca gaatagggtg gactatttgt ctcctctgtc gtttggttag
    16201 caaaatttga cgtttctgta taaacgttta cataaatact tgataagctc cctaagaaag
    16261 taaaaatcaa gatgatcgaa atcataatgg gatgtaagta ttttctttta gatacagctt
    16321 tgagtctaga aacaaaatgt ttttgacata tacgttggat tttattttga gttttcttaa
    16381 tcattttcat ccctcctctc aataaaaaat catcaaataa tcctttacac gttattctaa
    16441 atttaatttt tattatttta ttgatgtata gaaagtttgg ggcttttttt ctctttttat
    16501 tctaaggtta ttttagtctt gtcctttaat acaaagattt tatttttaaa taaaaaatac
    16561 tcttttttta cgattattga aaaaaaaaga taaaacttga ttaattgaag tattgcttaa
    16621 atttggaagc tatgagggaa gaataaaaga gttaggacgg gattatctac tatttttctt
    16681 tgttttaaat atttgttatc tttgaacata aaagagaaat gtgtcgactg cagtgttgtg
    16741 tccctcatca cgaataaacg gtgttcaatt agctgatctt gtattcactt aaaaaatggt
    16801 aaagtacgag ttgctattcc ccattttcaa ttttctaacg ctcaggacaa aatactgttt
    16861 tcacaacctc attggaataa aagcatatat attgtttcaa gaacgaaatc ttgttatctt
    16921 tttaaagggc atctgtccaa aatctaattt taggaggtac gttttggtat ttattcgtac
    16981 aaataaaaga gaattatctt tattcatgta acgcatccaa ttgatttttt catgtacgct
    17041 cctggaacga ttttactgac gattgcttat ttaactgcga atcgctcatt agcttttatc
    17101 atagctatcc atattttgaa taacgttctt ggctttgtat tatagataca gatgacaaaa
    17161 aaagttcaag tatgcggtgt agaaccaata tcgcatactt gaactttttt caatgagtat
    17221 aaattaaaac ttctaatttt caattcccat catctgatat gcccgaccgt ctgaacttct
    17281 tttgaaaaag ccaaatgtga ttagataacg tctttgttcg acataatcgc cactagaaaa
    17341 tacgttcagc agtatcgaat taacctctgt ttctgtataa gtcactccat ggtcaaagcg
    17401 tttcaagact tctttcaata ggatgtattt cttttttgtt gaacttggta cttgttttac
    17461 ttttccatca ttcatgaatg tgtctaaaac tttcatcttt tcttgctccg tataagtata
    17521 aaccatcttc tttctccttt tgattctaag aatactccat ttatgttaga atattctgga
    17581 ttttttatta aagcggttac tcttaagaaa gatattatca tagtgctctt ttttttacta
    17641 ctacttatag gtagatttat tctaatccat tattctcccc taccaataat taaagaaaaa
    17701 actggatcag ggtgttcaaa ttacatgcta agaaaaaaga acccttgaat accaagggtt
    17761 ctttctcagt ctgataacaa tgattaacga cggatttctt tgatacttgt gaaaagtcgt
    17821 taaagtattg atataaaagg atttttatta cctctgacta catattgact aatttgttaa
    17881 aaaatcgttt ttcaagaatc ctgtaatacc agtgctttca ccgttttttt aactctctaa
    17941 agaactaaaa gcaatcagag gttttacctt ttcatttgac gttaagtggc gataaatttt
    18001 cgaagttgtt tggtcagtat ggcctaagcg atctttcaag acttcccagc tacagtgttc
    18061 cgctactaag cttgccattg tatgacgagc taaatgagta tgaaatggct tattaattcc
    18121 tgagcgattc acgcaccgtt tgacgtgatc atttacatac tctcgacgat aaggaacacc
    18181 acgtttacta gaatcaagat acacaaacaa atatgtttct tctataataa acctgtaatg
    18241 ttgcctcatc tcacccaaag ccttaatcaa ctgttgtagc ttttccataa ctagaggagt
    18301 tactagaact cgtctagcag aactaactgt cttagtagtt tcaattcgaa aatccttact
    18361 cttaccttta actgccgttt gcttatagaa cagaatttca tttcttttga aatctatatc
    18421 tccaggctgt agagcaagcg cctcgctgac tcggcagcca gtgtaaaata gaacatggaa
    18481 aagtacagac atcggtaatt cctcatgaac aatgccataa ttcaaaaaag ttctcaattc
    18541 atcgacagta agaaacttgt cctcaatatt atttatggct tcttttaaat cagttaccgt
    18601 taactgatat tttggcattc tcacagactt cattggattt tcacttatca aattctttct
    18661 ttgacaatat ttaaaaatca tatttagaac ttgaataagt gattgtaaac tatttttggc
    18721 atagccaaca tttgttccat taacgccaca ttcctttaag gtaaaaagca attcttgaag
    18781 gcactctggt gtcaaatcag acaacttttt atcaccaatc catcgaacaa taatttttaa
    18841 ttgttcctct cgcttatcat aggttatttc cttttgttca tttaacgaac ggtaatattg
    18901 gaaccattgc tcacctacaa cactaaactt gtcattgctt gcaacaattt ttttcttaag
    18961 cctttgttgt ttctccattt ccagataagc gagttgcgcc tcacgttttg aattaaagcc
    19021 acgtcttgtt gttcgatatg cttgaaagta ccatgtgatc ctaccagtag aactgatttg
    19081 cttcttgaat cgatctgttt tttccattta ttgattcctc ctattttgaa ggaacccaac
    19141 aagcagaaac cctttggaaa tctttctatc tatgtcatca ttgtaccgca tcttagaaag
    19201 aaaatgtaac ttataaagat cgaagtagtc gcaatttttc tcgatagtca ttcgaatcac
    19261 tctttctcat ccattctaaa acatcatcac gaaaatagtg tcctcgaatc ccgtaccaac
    19321 agggaggaaa ctcaccaagt tttactaaac ggttcagtgt actatcactt atctgaaata
    19381 gtcgctttaa atcagtattt gttaatacca gaggtaaatg actgactgca agcaattttt
    19441 ccgtgtaatc ttccagtttt ttttgtaaaa ttgaatagaa ctctatttcc agagaattaa
    19501 catttgtttt aacttccaac tccatcaccc ttctctctca aaatcaaaac gaccaccacg
    19561 gtacccctta aaattgtaaa aaaacactcc agtactccat caaaacggac tagaaaagag
    19621 gaatttttaa tatgaaacaa ggaattgcag acataaaaat aattaaagaa attcttgaaa
    19681 aatctacggc taatgcaata gcattcggaa caggaatcaa cttgagtaca gtaaaaaaac
    19741 ttaaaagtgg agagagagct gaagaaaaat taaatcttgc cgatgctatt aaaatcacag
    19801 aatttggcat gaaaaatatg ccaaccaaaa tcgaaatatg gaaatcctag ctcttccaaa
    19861 gccatttgtc gttggtacca aacactgccg agacttttcc caataatatc gccaatttca
    19921 cgatcattaa gaccgatgaa gaaggacata aaaatgattt cttgtctttg actaggtaat
    19981 gctgcaatcg catttgccaa ctcctcattg gaaataaaga ttttcatacc aaggacaata
    20041 aaagtaccac tactattcaa atttggttcc aaataataga actctgtacc taaccctttt
    20101 tgttccaata tttccattga tatttgccgt tggcgaaatc tagcgtactt cttttgaata
    20161 tttaacgcct cattgcagac tactttctta caaaaactgt caaacgtatg ttgaatgtgg
    20221 gtttcctctg agtcgcccat aatattctcc ccctcaaaaa attcattcaa ctcgttttac
    20281 ttctatccct ttccattatt actacaatct agga
//
LOCUS       DAJSBM010000051        19821 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJSBM010000051 DAJSBM010000000
VERSION     DAJSBM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.051"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..305
                     /locus_tag="ONT67_001997"
     CDS             6..305
                     /locus_tag="ONT67_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355727.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ONT67_001998"
     CDS             378..1739
                     /locus_tag="ONT67_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR2355728.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ONT67_001999"
     CDS             1761..2021
                     /locus_tag="ONT67_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355729.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ONT67_002000"
     CDS             2035..2787
                     /locus_tag="ONT67_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCR2355730.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ONT67_002001"
     CDS             complement(2881..3486)
                     /locus_tag="ONT67_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2355731.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ONT67_002002"
     CDS             complement(3539..4513)
                     /locus_tag="ONT67_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCR2355732.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ONT67_002003"
     CDS             complement(4891..5610)
                     /locus_tag="ONT67_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2355733.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT67_002004"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ONT67_002004"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCR2355734.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ONT67_002005"
     CDS             7161..8558
                     /locus_tag="ONT67_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2355735.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ONT67_002006"
     CDS             8639..9079
                     /locus_tag="ONT67_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2355736.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ONT67_002007"
     CDS             9227..9553
                     /locus_tag="ONT67_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2355737.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ONT67_002008"
     CDS             9554..10303
                     /locus_tag="ONT67_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355738.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ONT67_002009"
     CDS             complement(10383..10838)
                     /locus_tag="ONT67_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCR2355739.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ONT67_002010"
     CDS             complement(11265..11540)
                     /locus_tag="ONT67_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355740.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="ONT67_002011"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="ONT67_002011"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR2355741.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT67_002012"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="ONT67_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR2355742.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="ONT67_002013"
     CDS             14770..17436
                     /locus_tag="ONT67_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2355743.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="ONT67_002014"
     CDS             17800..18552
                     /locus_tag="ONT67_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR2355744.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="ONT67_002015"
     CDS             complement(18574..19728)
                     /locus_tag="ONT67_002015"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCR2355745.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DAJSBM010000052        19476 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJSBM010000052 DAJSBM010000000
VERSION     DAJSBM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.052"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..996)
                     /locus_tag="ONT67_002016"
     CDS             complement(22..996)
                     /locus_tag="ONT67_002016"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR2355746.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="ONT67_002017"
     CDS             complement(1076..1951)
                     /locus_tag="ONT67_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCR2355747.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="ONT67_002018"
     CDS             2081..3988
                     /locus_tag="ONT67_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCR2355748.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="ONT67_002019"
     CDS             complement(4529..7807)
                     /locus_tag="ONT67_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCR2355749.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8078..8623)
                     /locus_tag="ONT67_002020"
     CDS             complement(8078..8623)
                     /locus_tag="ONT67_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2355750.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8635..9327)
                     /locus_tag="ONT67_002021"
     CDS             complement(8635..9327)
                     /locus_tag="ONT67_002021"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR2355751.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9346..9630)
                     /locus_tag="ONT67_002022"
     CDS             complement(9346..9630)
                     /locus_tag="ONT67_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355752.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9643..10206)
                     /locus_tag="ONT67_002023"
     CDS             complement(9643..10206)
                     /locus_tag="ONT67_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCR2355753.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10434..11096
                     /locus_tag="ONT67_002024"
     CDS             10434..11096
                     /locus_tag="ONT67_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCR2355754.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11220..12416
                     /locus_tag="ONT67_002025"
     CDS             11220..12416
                     /locus_tag="ONT67_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCR2355755.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12517..13893)
                     /locus_tag="ONT67_002026"
     CDS             complement(12517..13893)
                     /locus_tag="ONT67_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2355756.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13930..14796)
                     /locus_tag="ONT67_002027"
     CDS             complement(13930..14796)
                     /locus_tag="ONT67_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCR2355757.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14885..15910)
                     /locus_tag="ONT67_002028"
     CDS             complement(14885..15910)
                     /locus_tag="ONT67_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2355758.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15928..16602)
                     /locus_tag="ONT67_002029"
     CDS             complement(15928..16602)
                     /locus_tag="ONT67_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355759.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16786..17679)
                     /locus_tag="ONT67_002030"
     CDS             complement(16786..17679)
                     /locus_tag="ONT67_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCR2355760.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17753..19450)
                     /locus_tag="ONT67_002031"
     CDS             complement(17753..19450)
                     /locus_tag="ONT67_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCR2355761.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6158 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataataagtg tattttataa aaagaaaaaa
     7921 tataacttaa gttgttgata ataaagagat ttattaacgt aaaaagaata agttttttta
     7981 aaataacgta taaaaaagag cataaaacaa agtcgtttgt tttatgctct tcttttatta
     8041 ttttgtgctt tttattttat caaatcactt tgttttttta taaaatttct gcgcctagtg
     8101 tggactcaaa atgtctcaat gcccattcat gtccaacggg atcgaaactt gccacggcat
     8161 ctttgaaaat atggagtttg tatcctagat tatacgcatc gactgctgta tgcaacacac
     8221 agatatctgt acatacgccg gtcaaataaa tatctgtgat ctggcgttct cgcaaacgga
     8281 tgtctagatc tgttccacta aaagcggaat aatgccgttt gtctatccaa taaacatttt
     8341 cctcttgctc atagtcttgg tataaacttt ttaaagagcc gtacaattcc cgtccacccg
     8401 ttcctatcac attatgcgga ggaaataagc ggttctccgg atgaaaggaa tcttttggat
     8461 catgcgcatc aatggcaaaa acgacaaaat ccttttgatc aataaacttc tttgtatgag
     8521 cagtcagctt tttttcaatt acttgtcctg ctgtcccagt agtcagcttt ccatcctctg
     8581 caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt catcttagtc ctccttatac
     8641 gagatgtttg acaaattcga tcaccatttc tgctgatcgt ttgcctgcgt cttcgataaa
     8701 ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg ctcattgcac gtacgatcaa
     8761 aaatgggata ttgaactgac gagctgtttg tccaacagcg gctccttcca tttcgcaagc
     8821 taatgcttca gggaaattcg ttaagatttc tttgatttta tctggcgagt cgacaaatgt
     8881 atcacctgta acgatcaatc cttcttttgc gttcaaattc gttgcttttg ctgctttaac
     8941 catttcactg cgcaaatatg tgcttgcttc ataatataga ggcattcctg gtaattgtcc
     9001 tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa gctactttgt ctgatataac
     9061 gatgtcacct acttgtaagc cttcaccgat tccgccagct gaacctgtat tgatcaccat
     9121 gttcacgccg tattgttgga tcaacagact tgtggtaata gatgctaaga cttttcctat
     9181 accggaacgt acgacgatca cttcgtggtt acctaatgaa cctgaaataa ataaagcgcc
     9241 tgcgcgttcc catgataatg gttcgcttag gttctctctc aaaattttta cttcttcttc
     9301 cattgcgccg ataataccga ttttcatctt tttaattcct tctttctata taaatgcgat
     9361 tgctaaaaaa accacacata acattaggat aacaataata agagatttca tcaaaaaaga
     9421 gttccatttg gtcgtctttt cccgttcgct gatccttgtt ttcgtgattg gtttattctt
     9481 tttgctttct ttacgataaa agctggcaat ctttttttct tcttgctgat aagctgtttc
     9541 tgcttttcgc tgttttttta atgactcgct tgcttgtgct tgttgtcgct tgcgaagttc
     9601 gcttcgcgta ataagcggtc ctttggccat aagccgatcc ctctactttc ctttggtcaa
     9661 tagttcccag tattgaatcg cataaatcgt ctttgcatca caaatcaact gatctttcat
     9721 cgcttgttgc gcctcttcaa gagttagatg atacaactcc agtacttcgt cttcatcttg
     9781 tgctaaagga ttctcgacct tctctacccc ttgtgcatga taaatatgaa ggacttcatt
     9841 tgcaaaacct ggtgaaagat acatcgaagt aagatggctc agcgacttcg cacgatagcc
     9901 agtttcttct tctaattctc tagctgctgt catttcggga ttttgtcctt ctcctggatc
     9961 gatttttcca gctggaatct ccaaaatcac tttttctaaa ggttttcgaa attgtttgac
    10021 aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg acaccaccag gatgaaatac
    10081 cagttctctc ttcgaagttc ctccatctgg caaacgaact tcgtctacag caacatcaat
    10141 aatttttccg ctatagattt ctttgcgact gattgttttt tcttcaaatt gctcattgtt
    10201 cagcatatct tctcctgctt tctttgtaat cataactgct tttttttcat ttttcaattt
    10261 ttctcaaaaa ctccttatta gtttaccata gataaacctt agattttctc taaatataga
    10321 aaatccttct ttagtccgat actctttcta gtagtccgta tgctaaaata agtatgtaat
    10381 cgaataacga aaagaattca atactcaacg ataaatgaca tggagggttt attatgataa
    10441 aacgaaattg gccgtggctt ctagcattgg tcttgctatt tctctttgtc cgaaacaata
    10501 gtttttcttt tgttctagga cttttattga taggtgtagt tttagttttg tggggactct
    10561 ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc tgcactgact gacgaacttg
    10621 aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat ttcatttttt cgacaaacaa
    10681 tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa tatgcagcag acagccaaac
    10741 ttaaagcaat cgatctccgc catgatccag tcaaagcagc gaaagcttta ttcaaagaat
    10801 tggtgaaaga accaaaccga ctgcatgaag caagtcagtt tctttatacg catctgccga
    10861 atctagttga tttgacaaat aagtataatg agatcaatga ccatgagatc aaaaacaaac
    10921 agacctatga aaaactagaa gaaagcgcgc agatcatcga ccagctgtca gctttaatcg
    10981 cacaagatta tcaaaaattc gttgctgagg atctagatga tatcgacgta gagatatctg
    11041 ttgcgaaaca gagcttaaag cgtgataata aatatgaacc agaacaaaag gattaactct
    11101 tttgttcata aaagaagact cggaacattc agatgatgtc tggctgtttc gagtactttt
    11161 tctaaagtag agacattcat gtaagaatcg agaacagatt caacaaggag gaatttttta
    11221 tggatcataa accgactgaa acaaaagatg ttacaccagt taacgataca cttgaagatt
    11281 tattgaacaa cccgttttca actccaacag atacgctgac cacttcacaa caagcggaga
    11341 ttgatcagct ccaagagcaa cagacagctg ctcgtttaat tgacaaacta ccggcggaac
    11401 gccaagaaca agctaaacag ctagcagcaa aaatcgatgc taatgatgca cagtctgtca
    11461 tcagctacgg atcggctgcc caagcaaaat taagtgaatt ctcacaatcg atgttgaatc
    11521 atgtgcaagc acaagatatt ggtcctgtag gagattcctt aactgaactg atgtatcgtc
    11581 tccaagaagc caatcctgac gaactgcgtg ccggagaagg gaacatcttc cagcgtgtct
    11641 tcgggaaggt caaacaatcg atctatgaag tgacagcaaa atatcaaaag attggtgcac
    11701 aaattgacaa gatttcagtg aaattagata aagaaaaaga cggtctgcta aaagataatc
    11761 tgatgttaga acagctttac cagaaaaaca aagactactt tgatgcactg aatatttata
    11821 tcgctgctgg agaattgaaa atggaagaac ttcaaacaac gatcattcca gaagcgatga
    11881 aaagagcaga agaaactggc gatcagatgg atgttcagat tgccaatgat tacacgcaat
    11941 tcctagatcg tctggataaa cgtacgcatg atcttcgctt agctagacag atcacgatcc
    12001 agcaagcacc acaaatccgt ttgatccaaa acacaaatca ggccttggct gaaaagatcc
    12061 aagcatctat cgctacagcc attccgctgt ggaaaaacca agtcgtgatc gcattgacct
    12121 tgcttcgtca aaaagatgca gtaactgctc aacgccaagt ctctgaaaca acaaacgact
    12181 tattgaagaa aaattcagaa atgctgaaaa tctcagccat cgaaacagcg aaagaaaatg
    12241 aacgtggaat cgtggatatc gagacccttc aaaccactca aaatgatttg atcgaaacaa
    12301 tccaagagac attgcgtatc caaaaagaag gaaaagaaaa acgtcgtcat gcagaagtag
    12361 agcttggaca catggaagaa gatctgaaac aaaaactgtt ggatttaacc caatagatca
    12421 cctttcgctt gttactaaaa caaacgccca acaacagttt attacaactg ttgatgggcg
    12481 tttttcttat ctttcttcta acttctccat tggagtctat ttctcattca tcttgctgtg
    12541 tccatagcca tagccaaaat aaatgatcag tccaataacc attacgatac cgaacacgat
    12601 ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa gtcaagatgg agacgattgg
    12661 caatagcgga acgaatggca ctttgaattc tccagctttt ggtttgccaa acatttttct
    12721 caacttcagt aagcctagac taacaagcat caaataggcg agcgccatga tattcgttag
    12781 ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat acagctgcca caccagcggc
    12841 ataagtcgct tttttcggtg tacgatgctt ttgatcgatt tctgtcataa acttaggaag
    12901 taagccatcc ttgctgacag caaacagtaa acgagcaagt gaatacatca tcgagatcgt
    12961 taccgtcagt agcgttaaaa ttgcccctac agcgatcacc gcgcccactg ttccatgtcc
    13021 tacatacctc attgcaaaag ccactgggtc attgactcct agttcagtat acggaacgat
    13081 tcctgtcaag atcagcgtca cgatgatgta aaggacagta gcaatcagta tcgaaccaat
    13141 gattcctctt ggcacatctt tttgcgggtt tttgacttcc tcggctgcca ttgcaacggc
    13201 atcaaatccc aagaaagcaa aaaacacaag agctgcacca tctagcacac cggaaaaacc
    13261 aaaaggcatg aatggctgcc agttatcagg tttaacgaaa aatattccta caatgataaa
    13321 caacgcgatg ataccaaatt tgacatatac catggcatta ttcaaacgta atgctttctt
    13381 ggcttcaagc gacacgatgt aagtgacgaa tatcactacg agcaccgcaa tcaagtcaat
    13441 gtatgtacca ttttccggat tgtacccgcc agttaatgct tgtggaagtt cggcccctaa
    13501 acttttcaaa aaaccttgca tatagccaga ccatccggaa gcaacagagg aaaccgctag
    13561 taaaaattcc ccgatcaaca accaccctgt cagccaggca gcaaattcac caaatacaac
    13621 atacagatag gcatatggcc caccaatcac tggcactcgc gaagcaaatt ctgcgaaact
    13681 tagtccagac aaaatgacaa cgattgcagc taagacaaat gaaagtgaca gtgctggacc
    13741 agcattttga ttggccgcaa cccctgtcac cacaaatatt cccgtaccga tgatcgcacc
    13801 aattccaagc atgatcaaat cagacgtttt tagttctttt ttcatctcac tcgtatgatt
    13861 gagattgatc tctttcttac gaaacaaatc cattgaagtt cctccccatt ttaggttgga
    13921 aaacagcgat taaactgttt tcatataccc taaaaggata ccacctaaaa tgactaaaag
    13981 gcaaccaaca ataacataaa tgagctcttt tttcgttttt ctttcgccaa ggatgaaaat
    14041 cccacctaaa gtagagataa tgatccccat ttgtgaaagt gagaagccaa cagctaaacc
    14101 aagtgatttg actgttagaa gcatgcagat gtttcctaag ccccataata atccacagat
    14161 catgtttttc cacacaaact gatcgacttt aactttacgg aaagcaaaga agcttgcgcc
    14221 taagatcatt ccgatacttt gaggtaagat aattgacatc gcatccaaat tcgcccagtt
    14281 gacgatgatg gtatatacac cataaccaat cgtagagaag atcaatgctc taaaaccctt
    14341 accaaaatca agcattttat tatctgtatc tatttttcct tcgtcatctt gtcgggcagt
    14401 caaatacgca ccgcttacaa gaagagctaa agcaataata ccgtatacat aatctttcgt
    14461 ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta ttcaaaatta attgaaaacc
    14521 agtagacaat ggtagcccta cagaaacacc catgtacttc attccatgga actgtccatt
    14581 ttgtccaaca ctccaagcta agccggataa aaatccgatc aagaaaatcc aaggggtgat
    14641 tacaggacta acaacgaaaa agacaactaa agaaaacaga aatgcaccga tcgtcattcc
    14701 tagtgtttgt tgattggcat cgccaccaat tttaccactt actaaaccaa tactacccca
    14761 tgccaacata gggactaatg ctattaaaat ttccataacc aatctccttt tatttcacat
    14821 gataagaaac ttatcagcga aaacgttaac aacacaagca ctttttttcg tttctaacga
    14881 ttttctaacg tttttcatta taaaaattta agattgggaa aaataaagtc attgttgtcc
    14941 cttttccttc agttgattca gccgtcaaat ggatacctaa tttttcggcc agattttttg
    15001 ctaaataaag tcctaatcct gttgaatgtg ttttgctaag tcgaccattt ctcccagtaa
    15061 atcccttatc aaagatccgt ttcaagtctt ccgctgggat accgatccct gaatctttaa
    15121 ctgaaaggct gactccttgc gcttcctttt ctatgctcac aatgatgttt ccgtgatccg
    15181 gcgtatattt gatggcatta ctaagcaatt ggttgaagat aaaagcgatc catttagcat
    15241 cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg aatatttttt tggataaaat
    15301 aatttgcctg actgcgtatc acaggctgaa tgattccttt tagtccatat tcttggatga
    15361 ggtagtcccg cgagaaactg tcgagtcttg cataatacaa aacttgttct acgtattcat
    15421 cgatttttga taattcattt tctagtaatg tatacttttg atcgtcaata tcgaactcga
    15481 tcgaacgaac tagcaattta gaagcggaaa gtggcacttt gatttcatgg acccatgagt
    15541 caatataatc tttttggtct tgttgattac tgattgctga ttgcattact tcttggtgtt
    15601 cacggatcag ctgattgata tattcttcta ccagtttttc ttcttctgtc ctcccaccac
    15661 tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc taattttccc caccatcttc
    15721 tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa gacgccctca atcaaagcta
    15781 aataagcaat tgtgctcaaa tcaactttga tgtttggcgc cagccacatc acaaagatcg
    15841 tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca ctgatctttc aaatatttaa
    15901 aaaaattcat cttaaccgct catcctttca gggaacgata tatccctgtc ctacttttgt
    15961 ttcgatatat ccttggatac ctgcctgttc gatttttcgg cgtaaccgat tgatgttgac
    16021 tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac agtgctcgca gcaatttttc
    16081 ccgacttaag atctttccat tgtgcttcat caaaataaat aatagacgat attcattttt
    16141 gctcaaatca atgatctctc cgttgatttc catacttccg ttgtcaacat tcaacgtaat
    16201 gccattgtgg ctcatcattt cactagataa ttctgtgtag ttgtaagaac ggcgaagtaa
    16261 tgcattgatt tttgcaatca gtacatcgat ttggaaatgt tttgtcacaa aatcatctgc
    16321 ccccatgttc atagccatga tcatatccat attcgtattc ctgctggaga taaaaatgat
    16381 cggtactttc gatacttcac gtattttttg acaccaataa tagccatcca aaacgggaag
    16441 attaatatct agtaatacaa gctgtggttc ttctctttca aagtctgaga acacttgatt
    16501 gaaatcggtc gttccaaagg tttcaaattg ccatttctca agttcctcca tgatcaattg
    16561 tcgaatgatc tcttcgtctt cgactaccat gatttttgcc ataaaaaatc cctccatctt
    16621 tcttcttgct actacaatat caaagaagcc cttgtgaaac aagttggctt acctggaaaa
    16681 tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg atttaatcgc gtttgtctct
    16741 tccaagcagc ttaaatttat tatgttattc ttaataattg gtctattatc tgttattttt
    16801 ctttcgttgt ttttgtcttt taaattttcg ataacaccaa taaccgaaaa gcgttagcac
    16861 tagcaatata cctagaagga gtataagtaa tcgataaaga tggtattttt ttgtctgttc
    16921 tatctttgaa gagacttctt ctgtaaaggg aactcgtctt cctgtcacta gtaatcggtg
    16981 agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact agatcctgct tttctctaat
    17041 tgctaatgta tttgtttttg ttggaaggac aacaattttc tcaacgactt cataagcgag
    17101 catccgacca tacacttcaa gatagaatcg gtcatcttgc tccatttctt caagatccgt
    17161 aaagagtttt ttctcagcaa taccgctatg ggcggtaatc acggaatgtg tactctctcc
    17221 tccagttgga aaagaggtgc cttgcaggac tgtcgctcct ctatctaaca aaagatcatt
    17281 cgtttcatca aataaaggca agctaacatt gattttcgga atatagatgg ctccgatcat
    17341 atgttgttcg taatattctt ttcctggatt ttggacattt ctaaccgctt gctcaaaagg
    17401 atctttgact tgtcccattc ctgggattac tgatgtatga gcaagcgtct gatttttctt
    17461 ttcatgcact gctaattgtt gctcatgttt ttcttcttta tcgcgttcaa gctgcttttg
    17521 ataattctcg atccttcttt gatccaaata attgctgaga gcatctgcta aaaatggata
    17581 tgtaaaaact gcagctccgg caagaaacat cactgccata aataattgta ataaaagact
    17641 gatttgggtt tgaggtctat tacctatttt ctttttcatt ttcttcacct tcttatttaa
    17701 aaaaactgca gcaaccttta taaaaccgat tgctgcagct tatttttgtt gcttatactt
    17761 cagacttcat tttagattta cggtaccagc tgtacgctcc aatcatcaaa cttattccga
    17821 ttagtaagaa tgccaagagc ccgttcccac cagtagcagg aagtgacccg cgtttcatat
    17881 tcggtactct ttctggccct ggattttcta ctcctacgat ctgaatttct tcattatagc
    17941 ttccataaga gatgataaat tcagtaaagg ctgcctcacc tgttggaagt acataacctt
    18001 ctggcgccat ggtctctttc atttgatagg ttccataatc taacccttga acctcaaagt
    18061 atccatttcc atctgcgaca aattctgttg cttgtccgct tgttgcccac tcaattctat
    18121 ctgctcctga ttcataaaca gctaaaggcg actccccttt ataaaatatt gcgtattcag
    18181 aacggtcttc attccacaat tcgaatcttg catctttaag caattcactg cttgatgcat
    18241 catatttttc aaactttctt ccacctgttg tgactttctc tggcggctct actccttctt
    18301 cataatcaaa ttcatcattt cgtccgattt caataaccgc tgtgttgtta tggaaatcat
    18361 ccgcttctag ttctgtggaa accttcaaat tatacgtgat cgttaaatct cctccacgtg
    18421 catctgctaa tctacctaaa gtatctactt tggctactcc attttcttct gtcaaaatcg
    18481 tcacgacaaa cccgttgctt actacttcta ctatataatc ttgtcctttt gttaaaatcg
    18541 tctcgccggc tcgcacttct atttcctgat cataataagt tagaccaact gtaggcgtat
    18601 ctgtgatttt gtaatttttg ataacagcgc ttccgttttc aaggacagaa tcgatgaaat
    18661 aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc tctttctaga tcaatataag
    18721 aaatcgtttc ttgttctcca gcttcattaa taatcgttac ttgtctaagg tcgctttcct
    18781 ctacattcat ctgtttatct gcttctctga tttcgttttt cggatacaaa tgcacatctg
    18841 tattgtatgt gtctttccat gtagaaccat cccacacgcc atccaccata tcaggcatca
    18901 tcactggcat cataacaacc attggagacg cgattctgga aatatgagct ggcgaataag
    18961 tttctaagaa aagatagact ttatctctag aagaaggtgc ttcttccgtc acttggacgg
    19021 tagagaatac cgcttctcca gcttcattcg ttgtttgttt gtcaatactt gtcagtcgat
    19081 attttaaaaa ccaactttct gtgtgccgtt tttgaatcca ttcaatcgct tcggcagaat
    19141 taagaccgtc ttctcgttcc ggtgttaaag gattatctcg aatcaaatcg taatatactt
    19201 ccgttacgtc aaacaaatca aaatcgactc ctggaatatt ttcttcacca aaatccgtag
    19261 aaaccaatcc gctattcggt cgttcttccg gaacagatga gaaacctttt ttgtgtaatg
    19321 taatcgtcac ttcacttggt ttttgtgcga cttcttcggc tttgactagt tttccgttcc
    19381 cgaaaatccc tattcccgca gtaacgagta acagtacact cattcccgtg acaatcattt
    19441 ttttattcat ttttttctcc ccgttctttt tttatt
//
LOCUS       DAJSBM010000053        18740 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJSBM010000053 DAJSBM010000000
VERSION     DAJSBM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.053"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            256..531
                     /locus_tag="ONT67_002032"
     CDS             256..531
                     /locus_tag="ONT67_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355762.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="ONT67_002033"
     CDS             544..2616
                     /locus_tag="ONT67_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCR2355763.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="ONT67_002034"
     CDS             2781..4667
                     /locus_tag="ONT67_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCR2355764.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="ONT67_002035"
     CDS             4679..5626
                     /locus_tag="ONT67_002035"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR2355765.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="ONT67_002036"
     CDS             5646..6173
                     /locus_tag="ONT67_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2355766.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="ONT67_002037"
     CDS             complement(6236..6889)
                     /locus_tag="ONT67_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR2355767.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="ONT67_002038"
     CDS             7021..7863
                     /locus_tag="ONT67_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCR2355768.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="ONT67_002039"
     CDS             8021..8917
                     /locus_tag="ONT67_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCR2355769.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="ONT67_002040"
     CDS             8920..9633
                     /locus_tag="ONT67_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCR2355770.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="ONT67_002041"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="ONT67_002041"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR2355771.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="ONT67_002042"
     CDS             10168..10395
                     /locus_tag="ONT67_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCR2355772.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="ONT67_002043"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="ONT67_002043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2355773.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="ONT67_002044"
     CDS             complement(11181..11681)
                     /locus_tag="ONT67_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCR2355774.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="ONT67_002045"
     CDS             complement(11960..12358)
                     /locus_tag="ONT67_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2355775.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT67_002046"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="ONT67_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCR2355776.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="ONT67_002047"
     CDS             13471..14238
                     /locus_tag="ONT67_002047"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR2355777.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="ONT67_002048"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="ONT67_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR2355778.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="ONT67_002049"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="ONT67_002049"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR2355779.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT67_002050"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="ONT67_002050"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCR2355780.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DAJSBM010000054        18057 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJSBM010000054 DAJSBM010000000
VERSION     DAJSBM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.054"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            100..294
                     /locus_tag="ONT67_002051"
     CDS             100..294
                     /locus_tag="ONT67_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355781.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            563..862
                     /locus_tag="ONT67_002052"
     CDS             563..862
                     /locus_tag="ONT67_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355782.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(959..1393)
                     /locus_tag="ONT67_002053"
     CDS             complement(959..1393)
                     /locus_tag="ONT67_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355783.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1625..2335)
                     /locus_tag="ONT67_002054"
     CDS             complement(1625..2335)
                     /locus_tag="ONT67_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR2355784.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2325..3185)
                     /locus_tag="ONT67_002055"
     CDS             complement(2325..3185)
                     /locus_tag="ONT67_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCR2355785.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3190..3828)
                     /locus_tag="ONT67_002056"
     CDS             complement(3190..3828)
                     /locus_tag="ONT67_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR2355786.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3843..4142)
                     /locus_tag="ONT67_002057"
     CDS             complement(3843..4142)
                     /locus_tag="ONT67_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355787.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4185..5669)
                     /locus_tag="ONT67_002058"
     CDS             complement(4185..5669)
                     /locus_tag="ONT67_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR2355788.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5689..6150)
                     /locus_tag="ONT67_002059"
     CDS             complement(5689..6150)
                     /locus_tag="ONT67_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2355789.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="ONT67_002060"
     CDS             complement(6168..7235)
                     /gene="ulaG"
                     /locus_tag="ONT67_002060"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR2355790.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7453..8223
                     /locus_tag="ONT67_002061"
     CDS             7453..8223
                     /locus_tag="ONT67_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR2355791.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8496..9323)
                     /locus_tag="ONT67_002062"
     CDS             complement(8496..9323)
                     /locus_tag="ONT67_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2355792.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9891..11126
                     /locus_tag="ONT67_002063"
     CDS             9891..11126
                     /locus_tag="ONT67_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2355793.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12125..12385)
                     /locus_tag="ONT67_002064"
     CDS             complement(12125..12385)
                     /locus_tag="ONT67_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR2355794.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12412..13713)
                     /locus_tag="ONT67_002065"
     CDS             complement(12412..13713)
                     /locus_tag="ONT67_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355795.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14005..14463)
                     /locus_tag="ONT67_002066"
     CDS             complement(14005..14463)
                     /locus_tag="ONT67_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2355796.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14476..15807)
                     /locus_tag="ONT67_002067"
     CDS             complement(14476..15807)
                     /locus_tag="ONT67_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCR2355797.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15818..17260)
                     /locus_tag="ONT67_002068"
     CDS             complement(15818..17260)
                     /locus_tag="ONT67_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2355798.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17247..18011)
                     /locus_tag="ONT67_002069"
     CDS             complement(17247..18011)
                     /locus_tag="ONT67_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HCR2355799.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
BASE COUNT         5746 a         3731 c         2789 g         5791 t
ORIGIN      
        1 ttgattcttt ttatttatca taaagatata gatacataat cattttcact tacatatatt
       61 tgaatattaa ctaaaaacag caagaaagaa gaggatcaaa tgtattatgt agaagtaaaa
      121 acaaaaggcg tcaaaaacaa acaatatgtg aaaggtatca gtaacgaata tcctttactt
      181 ggttcatgga aagaagctgc tcctttttcc aaaccatgtg ccataaaaat aaagaacgag
      241 ctagaaaaag aactgacttg tggtaaagct gttgtagata tcatcgaaaa gtaaatccaa
      301 tgtatagaaa ggaagatcac acatgaaaag cgacgtacaa attgctaaga ttatagaatt
      361 agaagaagaa gacgcagtgt tgacaattga aaaaaaataa atgtttgtat accatatgat
      421 tatttcaata tgtatcctgg aattggagat ctcgtagagc tatatcaaga tgaatcaaat
      481 atacttgtca ttcatcatga aatatagaga cttataaaat tgtttatttt tataatgtat
      541 cagtatagaa aggaaataaa acatggatta tactgttgaa tttgacagtg aactacttaa
      601 gcaatttcca caagaagaac atttcatgta taaagaagca atttcccaca aagtgacaga
      661 gtatttggag cataattttt ttcgtataaa gccagtgcgt actcgttcgg caaaaggaat
      721 atacgagatg aaagtcaaaa ttaataaaaa aaattatcgc atagcatttg ctttaaaaaa
      781 aaatcatgtg ttcatttttt atattagttc gaatttgcaa aaaattgtat ttgataaaga
      841 ggtgagtaaa aaaataaaat agctgagcat tggaggatag attgagtgat actaagtcta
      901 tcctcttgtt tttctaaaga actaagctag aattttttct tttgtacgtt attatatgtt
      961 atttgtcatt accaaaaagc accttttttc ttctagtatc tatgacataa gaaataccac
     1021 ccatttcctt tacttgaaca aatcctactg attcataaaa tttttttgtt ttctcagtat
     1081 catctgtcaa taattcgatt tggactggtc cataactagt gatcttctcc aaagcttcat
     1141 tcaaaagcac tttgcctatt ccttggcgct gatacctcgg atgaatgagg atatcttgaa
     1201 taaatgcgat cgtcgcaaaa tcagtgatcc aacgaacgat tccgataatc ttcccttccc
     1261 cattacgctt tatcagagat tcagattgtt caaatgctct agctaacctt gcagtgtctt
     1321 ttgtgtaatt cgtccaacca acgctcttat ataattcaga aatctcatcg atcataaggg
     1381 aatcaatatt catttttttc ttcctttctt atacaattct ccctctaata atacagttct
     1441 ttgactattt aatctatcac atataactct cctgtaaaga ttctctcgat tccttaaaat
     1501 tcctctttcc ttttagaatg catacttttt gataaataaa aaggttgtga taaattttct
     1561 tcacaacctc tgtttctctc tatcaagtgt tatcttttaa ttttctaccc ttaccctagc
     1621 atctctattg cccgtagtat gcatactttc catgtttgcg aaaataatgt ttatctagta
     1681 aatattgcgg gatttcacat atattggggt tgatcagctc gttttctttg gccatcagac
     1741 aaatttcatc taagatcaaa ctgttttcta ctgctttcat aggtgaatcc ccccatgtga
     1801 acggtccatg tccatagaca agtactccag ggacttcgtt tggatctaat tttctttcct
     1861 taaacgtctc gacaatcaca ttccctgtat gaacttcgta agcttctctt acttcttctt
     1921 tcgttaattg gcgtgtacaa ggaaccttac cataaaaagc atcagcatga gtcgtgccat
     1981 aagctggcaa atcgcgtcct gcttgcgccc acatgacgga gtgcgtggaa tgagtatgag
     2041 taatagcagt tatatcttca aaagtttgat acaaaacaac atgtgtgggg agatctgaag
     2101 atggttttaa gctatcttct tctattatat ttcccgacaa atctgttacc accatttgat
     2161 ctgcttgcat gcattcatat ctgacaccgc ttggcttaat gacgatgact cctagctcac
     2221 ggtccacttc actcacattg ccccaagtca atttgactaa tccgtatcgc ggcaatgcca
     2281 gatttgcttc aaggacacgt tgcttcattg ctgcaatttt ttcttcacaa gacatcatag
     2341 ccagcctcct tcaatttaga acttaagtag tcttttgcct gctggatttc tcttcgaaag
     2401 tcttggcttt tttcactcca catttcaatc agaaatgttc catcataatc tagacgtttc
     2461 aaatgtctta ataatccaag aaaatcaaca caaccgctac caaaaggaac atcacgaaac
     2521 tggcctgaat attcttttgt tacagggtaa gtatctttta agtggatagc agtgatataa
     2581 tcgatccctt tctcaagttc tcgagccgga ttattttccg gccatgcgga caaattgcct
     2641 aggtctggat atacttgtag aaaaggtgaa tgaatctgtt ctttgatttc taaaaatttt
     2701 gaaatggagt tcataaatgg atcatccatg atttccacgg aaagaatgat gccatattta
     2761 gaagcttcct ttacgctttc ttttattcct tcgataaagt cttctctgga attcattgat
     2821 ttctcttcat aataaacatc gtatccagct atttggatta ctttgacgct tagattatgc
     2881 gccaaatgga ttgctttttt cattagttgt ttcgctgctt ttttcttttc tggatcagca
     2941 gaaccaaacg gaaatcgccg atgcgcactt aaacaaatcg aatggatacg tacaccggag
     3001 ctgaacatct cttctctcag ctcagctatc ttttcttcag accaatctaa tcgtgctaac
     3061 cgctcatccg tctcatcaat cgacatctcg ataaaatcga agtccatttc ttttacaagg
     3121 gaaaaacgtt ctttccatga aatatctttg ggtaacgctt tttcataaat cccaatttga
     3181 gccatttcct tacccccaga tacgaatgat ctcatctttg aagttttgtg cggccttcat
     3241 cggatcattt gctgctgtaa taccacgacc agtgataaag gtatacacat cgatcccttc
     3301 aaataagtgt aatgtgtgtg gatttaatcc accagttaca gaaacattaa agcctagttc
     3361 aatcagtttt ttgatcttcg ataaatcttt ttcaccccaa gtttcaccag ataacagcgc
     3421 atcacggctt tgatggtaga ttacctgccg gataccgatg ttatgccaat ccatggcttg
     3481 ttcaaaagtc caattgccgt agagttctac ttgcaattca cctatttctt tttgtgctgc
     3541 ctccatagtt ggaatcgttg cacaacaaat gactgtcatc cagtctgctc ctgcttttgc
     3601 aacattactc gccactgttc ctccagcgtc tgcacatttc gtatcagcga ctagtttttt
     3661 atctgggaac atactgcgaa tacagcgaat cgctttttgt ccttcctgta agcaaagaat
     3721 tgtgcccact tcaataatat ctactacatc accaactttc ataacatctc ctaatgcatg
     3781 ttctaagtca ttgtgatcta acgcaatttg caaattcggc ctgctcatct tagttccttc
     3841 ctttacttgt ttttttggta ccactttgtt tcagcaaatt tttcactgat ttctttgact
     3901 gacatcacat ttttgatacc gataactgta actccttttt tctctgcctg agtaaacata
     3961 ttcaaaaaat tgactggcgt aaaaactaca tcgtattggc cagctgaact ttttccctct
     4021 gaaatggcac aatgatggat tttggtaatt ggtacgttta gttctttcat tgctttttcc
     4081 acacttctca tcatcatcaa gcttgttcct gatccatttg cacacgatac taaaattctc
     4141 atcttaatcc ctccattatg agtgttgtct aatcagattt tcttttatac tctctgcttt
     4201 tctgcatatg tgtcatagtc ttctacaatc aaaaaataac catcaggatt tttccgatat
     4261 tgtaactgcg gaatagccaa taaaccaatg acaactacgg caattccagc ataacctagg
     4321 actttcatga ttactgtaaa caatggccaa gctgttgccc agtcgaacat cccgatataa
     4381 ccaccgtatg ttgacatccc aatccatgta gcgaataatg ctgaaccgcc tacttgtatg
     4441 attcctgaaa gaaacgggaa caggcaagcg gctttgaatc cacctcggtt attggcataa
     4501 actgctataa cggcattatc aaagaacaac ggaatgaaac cggctatgac gatcgttggt
     4561 gatttcaata aaatcagtaa accaatcatc aaaaactgtc ccaaagcacc aaacaagaac
     4621 ccaatcgtta ccgcatttgg cgaaccaaag ccaaaagtag cagctacgtc aattcctgga
     4681 acagctcctg gtaaaatcgt gtttgagatt ccttggaaag attgagttag ttcatcgacg
     4741 aatgtccgaa cacctaattg caagatcgcc agatataccg caaaatttaa agaagtcgtc
     4801 ataatgtaga ataagaaact ctgcccttct tgcatgaatt gcgcttgaat caaataatct
     4861 ttgcctagta caatcagaat gatcccaaag aaaaatagca tgagtaacga agtagcaacc
     4921 atattctcat tgaaaatcga caagaatcct ggtaatttga tatcttcaat ttttttgtct
     4981 tgcttgattg atctattttt cttttcatcc cgctttttca tccactcagc taattttgca
     5041 aatataaaga ttccgaacat ttgctgatgt gccactgcaa atccggcacc ttctgtcaat
     5101 tcctgcgtga ttcctactgt taagttagaa gctactgtcc aataacaacc taaaatcagt
     5161 cccataaaaa tcaatgcttg tatcctacct agatcaggaa agcaaaacag taaaatccaa
     5221 aaagcggtag ctgcttgttg aacctgaacg tttccggtag tgaaaacgga acgcagtttc
     5281 gtatatttct gaaaacggac taataagata tttaaaacaa atgcaatcag taataaaatc
     5341 atcacatctc caaaggtacg gccaaatgtc tccattaatc cagcatccac agcattttgt
     5401 ccaaaataag gatctgtaac catggcatcc aaattgaacc gttcctttag tccaaccaag
     5461 attggccgaa aattattgac caaaccacca gagccaacag tcaaaataaa gtaaccgacc
     5521 gttgccttta agaatccagc gatgcattca tgaagcgggc gtttcagcaa aatatagcct
     5581 aataacacga taaaaccaat caggtatgct ggctgcgtca agatatttgt tgcaaaatac
     5641 gtccaaatgc ttaacaaaaa ttcccccatc ttttttctcc tctatcaatt aattatattc
     5701 tgccatcaca acttggtaat catcgatgga gttcgtagag atgagacgct caaccaatcc
     5761 atctgtcatt aataattcgg ataatttttg gatattttct agatgttctt ctggatcttt
     5821 tgctgcgagt gtaaaaaaca aactagccgt tttctcctca ttctcccctt cactttcaaa
     5881 acggattggc tgtttcaact tggtaaatga aatagctgtt ccaaaaacac catcactttt
     5941 ttctgaagaa tgcggcattg ctacttgagg tgcgatcaca atataaggac cgtatttttc
     6001 tacacaatgg atgatctcct ccacatacgt ttcatcaata atttccttat cgatcaatac
     6061 aagacagctt tcggcgatag cttcacgcca atccgtgatt tctttatctg ggtaatgaat
     6121 caattgatgg tcaaaaaagt atttcagcaa gaaactcccc ttctttctta taaaaacgac
     6181 ggaaacggtg tatcattttc aatcgagaag acatcttcaa atcctcgata aaaattaaac
     6241 tcaagatcat ctttgtttgt cggatagatg aatttcccgc ctacttgcca gatataaggt
     6301 ttaaatccat acttcaggcg atcctttttg aatttccaaa tttcagtgat ctcttttgga
     6361 tcagccataa aattagacca aatatcgtag tggacaggaa tcactacttt tgcattcaat
     6421 gactccgcca tgcgcagcat atcaacagaa gtaactttgt ctgttatccc tcgtgggttt
     6481 tcaccatagg cccctaaaca gacatcgatt ttgtgttcat taccatgttt ggcaaataaa
     6541 ttggagtaat gtgaatctcc tgcatgataa agattccctc ctgatgtttc gaataaatag
     6601 tttactgcaa tttgatccat ctcctgaggc atttttcctt ttaatacctc ttctggatct
     6661 tcacaagtga ccaacgctgt ccgatcaaac gcttctaaag ctactacatt gatgtctttg
     6721 attttgactg catcacctgg ctttactgta atcgtttttt cttcaggaat tccccatttt
     6781 tgccacgtgc gaaccacttc ttttggccca atgaatgttg cttcaggaca attttgatga
     6841 actgctgcgg ctgtatggat atctagatga tcggaatgga tatgtgtaac gattaaagcg
     6901 tctacatttt ttatcgcaaa aggatcgatt acgaaaggct gagtacgtaa attaggctgc
     6961 atattttgaa cgccactcat acgcatcatc tgatggcctt ttttcatcat gccattccca
     7021 tgactacgtt ttcccgtccc acaccataga tcacataaaa tattcgttcc ttcatgactt
     7081 ttcagccaaa ttcccgtaca gcccagccac cacatagcaa aagttccagg atccactact
     7141 tctgtttcga tttcttcatt caagtatgtg ccccactcag gaaatgtttc tactaaccac
     7201 ttttcttttg ttacttcatt gacagtttta ctcatttgct tgctcccccc atatcgttgt
     7261 tccaacttac aagtaaatat taatgtgtat cagcatccat gaaaaccctt ttatatgacc
     7321 gtttgttttt tatatgattt tctttcagta atcaatgatt gtttcagtat aataaagaca
     7381 gatatgaatg tttaaatgaa caactagtca aataaatcca tggatcaaga tgtccctcag
     7441 gaggcaaaag tcatgaaaaa ctcacttgtc aatattgaaa aacgtcaaaa agaaattttg
     7501 gaactgcttc agcagactga acaactatca actttcgaat tagctgaatc ccttgatgta
     7561 tctattagta cgatacgaag agatttaaat ttattagaag aaaaaaacga gatcatacgc
     7621 aattacggtt attgttctta taattacaaa aaccagactg attttgatca atctggtcct
     7681 gaaaggatta aacaagcgat tgccagagaa gctagtaaat acattaacga ttatgatacg
     7741 ctgtttatca attctagctc gactgcgttg aaaacactgg attttcttca atctaaccat
     7801 ttgacagtca ttacaaacaa cgtgaaaatc aattactctc cgcacaaaga taattgtagt
     7861 tatattttaa ctggtggaga gatgcgcttt ccaaaagaag cactagtggg cgacatcgca
     7921 ttaaatacga tttcttcagt caatgcagac atttgtatca ttggctgtag cggagtcgat
     7981 gtagtacatg gggtcacaac aaagatatta aatgaatcaa cgatcaacga aatgatggtc
     8041 aacaagacaa tcaaatgtaa gattttagtc gctgatcatc gaaaaatcgg actgacatca
     8101 aagttcaaga ttgctgatat ttcagtcttt gattatttga tcactgataa attttgttct
     8161 gaaaaagtat tagaggagat cagaaatacg gggataaaaa ccattcaagt agattcattt
     8221 taagaaccag gacgtaaata tgtaaatagg attatcaaaa aaatgaataa caaatttatc
     8281 gataaatgaa aaacacgcta cgctcaaatt gataaaggaa tcccaagatg tatccatcca
     8341 atcgatacca tcataatcta ttgcttcttt tataaccgtt atatgttgcc taaataggaa
     8401 tcaaacaata tcgaaaaaag gaggtatgac aaaagtcaca cctccttttt tcgaataaac
     8461 ggtattcaaa tcagaacact gatttcacaa ccactttatt tcaggtcgtt ttcaatcacc
     8521 tttaccgcgt aatcatttcc gttgaaacgc gctatttttt ttgcgtccat atatctttct
     8581 aaataatcat cttctcctaa ttgcttatgg caagaagcaa tttcataaag caagtattcc
     8641 aatgcataag tcgatttatg tttcttatta tactcaatac ccttctcaca taattggatt
     8701 gctttatcat aatcctcgat tttacggtaa taggtggcga tatcataata tacaatgcag
     8761 accatcagat tatcaaaatc agtctcactc aatattttaa gtgtacgctc cacataaact
     8821 ttcgcaaaat ctaattctcc ttttgacaag tagatcattg ccacgtttgc agctgcttgt
     8881 aagtgatgga tatctgtttc ggtcgtttgt tccaacactt gattaaaata aaataatgct
     8941 gcatctggtt ttttcgcata aagatgatca agcagtcctt gataaaataa aatttgattt
     9001 aaaagatcag aattacttac tttttcctga tcgatcgttt gtaaaatttt ataagattct
     9061 tggtattttg atacagaatt caatgattga atctcattta tttttttcct gattttgtct
     9121 tcttctgttt ctactaatat atcggagatc tcgacattca gctttttgca aagcgcactt
     9181 aagatattga tgcctggcga catattcttg ttttcgatat tgctgacagt tgcttgttca
     9241 caaatgcctt ctgctaattg tacttgtgtt aacccctttt tctttcggta ttcttttagt
     9301 aatgctccat ctactttaat caaaacaatt tccttccctt taaaaaatta tttctccttt
     9361 atatcataca caaaaaaaag ttctaaacaa ccaaaaatgt ttagaacaga ataaaaattt
     9421 taattttaaa ataatattca aattaaacaa ctggcgtatc tgctccttga tttctttttg
     9481 tgttatctga ataattatcg tttacagtta taacacatag tgaaaattgt catggcgaga
     9541 aagtctaaga atatttttaa acataactat ttctttctta cgtgtgtata atgacgacag
     9601 cacacctatc aattaatata attattttat ttttttatgt tctcatgttt atttttaatg
     9661 acacaacttc tcgtatttat ctggcacaac atataaatcg atcaaaaaat tgttgcgtgt
     9721 ttttttgaaa attggagttt caacttgatt acaaaattta aatttagaag ttgtgaaaac
     9781 agcgagagac agtttaatat tcaaaatata cataggctac tcgaacgctg tgttttttag
     9841 tatactagta agaaacgaag ggatagactc attccaataa ggaggatttc atgaagacaa
     9901 ttctttttgc tcctgcagta ttcaatttag ctgagacaac acgaatgatt gaggttgcca
     9961 aagctatgaa agaccgttat tactgtgtat tctttggctt ttctaaaact tttgctcctt
    10021 tgatcacaga aagtggcttt gagtatcatt tactagcgcc gatccttacg aaaaaacaag
    10081 aagaacaaat cataaaattt gaccagttgc gtacacttaa aaaccctttt accaaagaaa
    10141 tggttgcctc tcgtatcact agtgaacaga gtctgattgc taagtttgag cctcgactga
    10201 ttgtgacagg ttcgaatgtg acaatttttt tatcggcccg caataaaaag attcctttag
    10261 tatatgttaa accctatgct ttgagtgaac ctttttttca atcagagcat gcgctgatgc
    10321 cccacgagtt acgtttcttc ggccctttaa gcaatcccct ttggcactgg acaaaaaagc
    10381 gtatagtaaa tacccgatgg atccccaatg ccttcaaaca cgctgcacaa aaagaaatgc
    10441 ttcctttacc aaaaacaagt ttacaaatga tggatgctga tctaaatctc atcacaacgc
    10501 ctaaactatt tttggaagaa gaacagctgc cggaaaatta tcgaatcgtt ggtccaattt
    10561 ttgctaagct cagtacttcc ttgcctgaag aagtgctaac ttttatacag cgaaagcaac
    10621 aagaaagaaa acgtattatc tatttcgcaa tgggtagttc aggcaatccg cgtatgttac
    10681 gcaaaatttt gttcttctta caaaaacaac cggatctagc ggttattgca cctattgact
    10741 atctgcttga tgacattcaa tctgactcag aagatctttt aataacagaa tatctgtcta
    10801 gtcatctttt gcatgaattc attgattttt cttttatcca cggcggggaa gggaccgtcc
    10861 aaactgcctg tgaatccggc aaaccgttta tcggtgtggg gatgcaattt gagcaaagct
    10921 gtaatattga ctattgtgta aaatacggaa acgcaatcgc cctcacaaaa ccagtgactg
    10981 aaagaaaact gcagattgct ctgcgcgaag tttcttcacc caagattcgt cagcaggcac
    11041 gcacgctgca agccgttttt ccaataaatg gtccacaaca agcaattcaa gaaatcgaaa
    11101 ccttcctgag caaccaccca atatagtttc aagtagaaaa agctttttta atagagactc
    11161 atcagtaatt tcgctattcg agacccagac agtgaaaata caaaaaaagt aaatagtgga
    11221 ttgaaaagtt agagttactt aaattacggt gtctaaaaaa cacaaaatca tccttaaaat
    11281 aaaagatctg acgctatcca aatgagcata ggtagcgtct gccatttatt cttagtcaaa
    11341 ttctttgaca cctttctctg gttggatcat ctcaacgttt cttcaaaaga aagaccataa
    11401 taattaggca aatacatttt cttaaaatgt gtcatataga tctagaagca tcgctttgta
    11461 gagatttgtg ccgctagtcc cctcaacaat tactaaatca aatgtaggat ttaccacttt
    11521 atctttgata atcaacctcc ctcttctgtt gagacaatga tctcgcaatc cgatgcatac
    11581 tcctatataa actgccagca atccccacat attgagtaga tcatgtcaga atgttctatg
    11641 tcgtcagcaa cgatacacta aaagtcacgt tcaccatgac tgattgctat tcccagcaca
    11701 attatttcta tacacgtttc atgatttgaa taaacactca catcatacat attaccattc
    11761 atacaccata ctgcagcccc tactatatgg ttgtaagctc ccaaatctat atttgacttg
    11821 atcactttgg tagtagatct actaattcta aatccattgc tgataaaatt tccatttttt
    11881 cctccttctt tctgccaaaa cagttaaaac aataataatc tttatgtaaa aaagggctgt
    11941 gacaaaagcc ttgtcacagt cccatattcc gaataaacgg tggtcaaaag cttgcatctg
    12001 cggtaataag ttcaaccaaa cgaaaaataa gccaacattt cacaaaaatt taagaagcaa
    12061 ttttcgtaaa tttcttctta tttcttgaag ctaaccactt ctgttacaac cacttttaga
    12121 acatttaaag cttcgatgtg acaaaccatc tatgctgctc gatcataaaa ttgatagcaa
    12181 aaattccaca aagtacatat gtcccccatg aaaacaaaac attcatatgc ggtacaatga
    12241 tctttggaag aatcacgatc aaaaatgatg ccagtgccca aatccatcga ttgatttgaa
    12301 tatgatatgt agctagcata ttcaatataa ggtgactagc cgttaataaa aagataaaaa
    12361 caaaaaataa aatcagccaa tacattgttt cccaccttct aaaaaccaaa cttagacagt
    12421 ctggacttcg ttttctccac gactttgttc atctgacagc atctttttat cataagcttt
    12481 tacaaatgga taatagataa taaatgctat caatgtgttg atcatgatca agataacaac
    12541 acgccagtca tttcctgctg aaagataacc gccgattgga gctggtaatg tccatgttgg
    12601 caaaacagta aatctattga ccatttctaa tttgactgca aaataactga taatagtcaa
    12661 aactaaagga actaagttga acgggatgaa catgtaagga ttcaacatca taggagctcc
    12721 aaaaattatg ggctcattga tattgaagat accaggaata atagaaaaac gaccaacctc
    12781 tttcaagaat tttgatttac agaagaatat aaacaaaaca gataccacga tcgttgctcc
    12841 tgcaccacca attgttaccg tccattgata aaactgttca ggagcgatat agggtaattg
    12901 ggtggcagct gtaccatctg ccaatgcctt tgcattctca tccaacatga tcagccacat
    12961 tggacgaaca acagccccta caatactcat gccatggata ccaaatgacc acaacaaatg
    13021 gatcaaaaac attggtagta aaacaccaag caaattattc cctaaaatat tggttaaagg
    13081 ctcgaataac gataataagg cagcgtttac atcaaagccc gcgacgcttc gaatcagcca
    13141 agtaattgtt aaaatgactg cacctggaat caatgcctca aaactacggg caacagcagg
    13201 tggcacttgt tctggcattt tgatagtaat atttcgattt ttaaagaaac gatatacttc
    13261 cactgcaaaa atcatagata agatccccgc aaacatccca gatgctccca aagaactcat
    13321 aggtaagacc cagccaagag gtgcctcttc aggagttaaa acgtttacag gaacttgtaa
    13381 cataaagaac gttcctaaag ataatatacc acctgtcaca ctatctaatc catatgattt
    13441 agctagatcc gaacccataa caaaagttgc ataaatagcc atcaatccca tcgtcatcct
    13501 aaagggtaaa acaatcgttg ccgaataagg ggcaataaat tcattccatc caggaaacgg
    13561 taaattgata aagaccataa aaaatgtccc aatcaaaatc aatgacaatg ttgaaacaac
    13621 cccattacga attgctaata aatgacgctg tgtaccaatt tttgccattg gtgggacaag
    13681 tttctcttcg acccatttca tcagcttttc cactaaaacc actccttttc aataaaatat
    13741 gaaccattca ttgcatgaca acgcaaagaa tgttcataac taattatata attaagaaat
    13801 tttatttcaa tatattctct taaataatca aatttatttt taagaatcct gaaaataagt
    13861 tttctaaatc ataggagcaa caaaaaaatt cgataattaa caaaaatcaa aatgattaaa
    13921 tctagtgatt gaaacatatc atttcaaaaa aattttgtaa attcacccat aaatatcacc
    13981 ctatttaact tttttcatcg tacactaaaa ttcaaaacaa ctatctttaa tctttacttc
    14041 ttggttttgt tttaataaat ttccttcaaa aatgcctgga gaaactacaa tgatcaccgt
    14101 atctttttgt gcttgctcca acttcttcaa atctataata gctaacagtg tcccttcttt
    14161 tacctcctgg ttactttcaa cccgtatctg aaacggactt ccttcgagtt caactgtatc
    14221 taaacccata tggatcagta tttttctgcc agattcactt tcgatcgtta tggcatgtcg
    14281 ggtaggaaat atattatcga ttattccttt aactggcgca tggatatatc catcatgatt
    14341 cgttatagcg tatccaactc ctatcatttt tgaagaaaag acctcgtctt ttactgctgt
    14401 taatggaata atttgaccac tagctacagc ttttagaatg tttcttgttt taaagaaatg
    14461 cactttttat cacctctact ttaatgctgg ccaatatttt ttgtttactt tgatcatttc
    14521 atctagtatt ttttttgcga ctctggcatt tggtacggtt tgattcaatg taaatgcttg
    14581 taaagctttt tgataagaac cttcaaagta agcatctact agtaacttct ctactgcaac
    14641 ttgtgcctcc atcaatcctt tatgaaaatc actgattggt tttcttagtg taattggctc
    14701 cacaccagtt gcaccaacat atgccggtat ttcaacaact gcatctgacc gtagattcgg
    14761 aatcgcccct tgattaggaa cgattagcat aaagcgacga cgatcatcat tcaacaagga
    14821 aatagctata tcgacgatat attgtccatg ctccccaaaa ttaaagttca atacttcttc
    14881 cacaataccg cggcgtattt tatcagccat ttctttggta tttttctctc gtccatccat
    14941 gaccatatta gcacgtgtat actctttgtc agtatgctca acgaccatat caggatataa
    15001 gtaatactct aagtaattat tcgggatatt acttggaaaa tttttcgtga tgactgacat
    15061 catttgaaag gttttttgcc aagttttatc tcctatatta aaatcggcga cttgcatttc
    15121 ttttgttatt agtttttcaa taatctctgg cataatttct cgttgcaatt ctttatcata
    15181 aatcgatgta taccagccaa agtggtttaa accataatac gtaacgatcc aattcttacg
    15241 atcataaccg taattcactg caatcgtttc ttcaattgaa atagtcatat cacaggcatt
    15301 aatcattttt acattcggat atttacgacg aacagcttca gaaacgatcg actctggatt
    15361 tgtataattc aaaatccatg ctttgggggc atagtcttgt atatgatcga caatttccaa
    15421 taaacctttg attgagcgca tcccataagc aaatcctccg agtccacacg tttcttgtcc
    15481 tactaatcca tgtttaagtg gaatcttttc gtccttttct cgcatctcca atcctccaac
    15541 acgaatttgt gagaaaacaa aatcgcatcc tgtaaaagca agctttggat catccgttgc
    15601 acgaatgcga atatttgaaa aaccttttct tttcagcata aatttaacaa tcaagtacat
    15661 atccatattt ctttcttcat caatatcata taaacgtatt tcagataatg gcaaccgttt
    15721 ttcattgttc aaaatggctt gaacgattcc tggtgtatac gtactcccgc ctccagctat
    15781 agtgataatc tgttttttca tcatcatctt ttcctttcta ttcttctaat aatttattga
    15841 attctgccat aacataagtc acttctaagc cgatgataat ttgataattt ttttcagttg
    15901 gtttaatgat cccgcttatc cctaattgct tgattcgctg ttcctcgacc atggtgggat
    15961 ccactacctc tacccgtagg cgtgtattac aactatagac atccaggata ttggcttttc
    16021 ctccaaacgc ttgtagcatt tttgttgcca tatattcata atttttactt gctaacccta
    16081 actctgtttc ttctaaggtt acttcattgc caaactctgc cttagcttca cgtcctaatg
    16141 tctgaatgtt tctcttattg atcaaataat aaaaagtaac gtaatacaag gcaaaaaaga
    16201 aaattccgat tggaagaatc aaaatagcat tggtagcgat tcgaaaattg atgagataat
    16261 caacaataca tgctccgaat gtaaaaccaa ttcgtatatt gaataagtaa cagacaatcc
    16321 ctgctaaacc ggcaaaaagt gcatggatca cgtacaacat aggagaaaga aacatgaaac
    16381 taaattcaac aggctcagtg atatttgcaa taaatgaagt tcctgcaccg cttcccatga
    16441 ttcctttgac catctcttta ttttctttat aagctgcttt atagatagct agacatatag
    16501 ctggtatacc aaacatcatt actacaaaaa aaactgataa aaacagtcca gcctgaggat
    16561 ctccgttgat aaagcgtgtc atctctcctc tgaaaacttc cttgctagga gatgtatagc
    16621 ttcctaaatc gtaataaata tacgtattca acacatggtg cagtccaaag gggatcagca
    16681 aacgattcaa aaacataaat atccccacac ctaatgctcc catatcaacg agcagttttg
    16741 caaaattatc aatcccaatt tggatgagcg gccagataaa gcccaaaaaa acagctgcga
    16801 ttgtttgtag aatcatcacc atagtcaatg ggaatttttc gcccgcaaaa taagaaaaaa
    16861 ctactggtaa tttttgttct ttaaagcgat tatacgtcca tgccgctact aaaccggaaa
    16921 taattccacc aaaaatcccc atattcactt ctgggtccat aatttctaag ccttttttca
    16981 aggtagcaat cgctaaaaaa ccagtaagtg caggtattcc tttgtcctta tttttgacaa
    17041 aaccgattgc aactcctata gcaaataaga tatccaagtt tccaaaaacg gcatttccag
    17101 ctgcaccaat tactgggatg ttcagcatat cgtcagctga taaacgcatg atcaatcccg
    17161 ctaatggcat cgctgcaact ggaagtagca tcgctcttcc aagcttctgc acatttgatt
    17221 taacattcat ttttattcct cctctattaa atatcctcat atgttactaa ctcaatcaaa
    17281 ggatcatttt tcactttttg agcaaatgct gaatgaatta aaaattcgac ttggtgggta
    17341 cgaggataca caaaacttga accatttaac aattcctgat ccaaatacgc tgcgtgacac
    17401 atgacttcga tcgttttatc ttctctcaat atcatgagta aacttgccaa atattcttcc
    17461 acactcgtat tgatttgtcg ttttttttca tcagtttctc ctacgacatc gaaacaaggc
    17521 tcaaaataat cgacatgacg aacttcatct tttcgttcaa agttgctccg aaccggcaaa
    17581 tcatattttt ttgctaatgt aacaactacc tcctgatttt catccaaagt atgagtatga
    17641 tgatgactgt ctaaatgagt tggtatgatc cccgcatgat aaattttttg aatctgagca
    17701 ttccattcct gatacagttg atctttgtca atgttaaatg gatgctgata ataagataag
    17761 gatctgaact ctccgctttc gataagagta tcgacagttt tgaggagtgg tttgccacac
    17821 gtcaatgtta aatggactcc tacacctagc ctaggcattt cctttcttag tttcacagca
    17881 tgctcgaaac ccggcatatt tgccatcaat gtagttgatg tcaaaattcc tcgctgataa
    17941 gcatccataa tcccataatt cacaccatgg ctatagccaa aatcatccga attaaaaatc
    18001 acttttccca tttcattctc cttctttttt ctactaaaat aggcgcaacc aaataat
//
LOCUS       DAJSBM010000055        18019 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJSBM010000055 DAJSBM010000000
VERSION     DAJSBM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.055"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(27..725)
                     /locus_tag="ONT67_002070"
     CDS             complement(27..725)
                     /locus_tag="ONT67_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCR2355800.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="ONT67_002071"
     CDS             complement(718..2070)
                     /locus_tag="ONT67_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCR2355801.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="ONT67_002072"
     CDS             complement(2293..3693)
                     /locus_tag="ONT67_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCR2355802.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="ONT67_002073"
     CDS             3854..4591
                     /locus_tag="ONT67_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR2355803.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="ONT67_002074"
     CDS             4716..5195
                     /locus_tag="ONT67_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCR2355804.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="ONT67_002075"
     CDS             5192..5959
                     /locus_tag="ONT67_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355805.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="ONT67_002076"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="ONT67_002076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR2355806.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="ONT67_002077"
     CDS             complement(6862..7464)
                     /locus_tag="ONT67_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2355807.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="ONT67_002078"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="ONT67_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="ONT67_002079"
     CDS             8145..8729
                     /locus_tag="ONT67_002079"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2355808.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="ONT67_002080"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="ONT67_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR2355809.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="ONT67_002081"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="ONT67_002081"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR2355810.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="ONT67_002082"
     CDS             10630..10809
                     /locus_tag="ONT67_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355811.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="ONT67_002083"
     CDS             10831..11856
                     /locus_tag="ONT67_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR2355812.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="ONT67_002084"
     CDS             12031..13275
                     /locus_tag="ONT67_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR2355813.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="ONT67_002085"
     CDS             13428..14375
                     /locus_tag="ONT67_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2355814.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="ONT67_002086"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="ONT67_002086"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR2355815.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="ONT67_002087"
     CDS             complement(15277..15519)
                     /locus_tag="ONT67_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355816.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="ONT67_002088"
     tRNA            15640..15712
                     /locus_tag="ONT67_002088"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="ONT67_002089"
     CDS             complement(15831..16469)
                     /locus_tag="ONT67_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCR2355817.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="ONT67_002090"
     CDS             complement(16592..17224)
                     /locus_tag="ONT67_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR2355818.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="ONT67_002091"
     CDS             17575..17874
                     /locus_tag="ONT67_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2355819.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6169 a         3154 c         3558 g         5138 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaatta
//
LOCUS       DAJSBM010000056        17224 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJSBM010000056 DAJSBM010000000
VERSION     DAJSBM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.056"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..369
                     /locus_tag="ONT67_002092"
     CDS             1..369
                     /locus_tag="ONT67_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR2355820.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ONT67_002093"
     CDS             549..1490
                     /locus_tag="ONT67_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR2355821.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ONT67_002094"
     CDS             1559..2290
                     /locus_tag="ONT67_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR2355822.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ONT67_002095"
     CDS             2434..4614
                     /locus_tag="ONT67_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR2355823.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ONT67_002096"
     CDS             4634..5116
                     /locus_tag="ONT67_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCR2355824.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ONT67_002097"
     CDS             5132..5893
                     /locus_tag="ONT67_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR2355825.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="ONT67_002098"
     CDS             6024..7358
                     /locus_tag="ONT67_002098"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR2355826.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ONT67_002099"
     CDS             7665..8150
                     /locus_tag="ONT67_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCR2355827.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ONT67_002100"
     CDS             8143..8826
                     /locus_tag="ONT67_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR2355828.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ONT67_002101"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ONT67_002101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR2355829.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ONT67_002102"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ONT67_002102"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCR2355830.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /locus_tag="ONT67_002103"
     CDS             12442..13071
                     /locus_tag="ONT67_002103"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR2355831.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ONT67_002104"
     CDS             13123..13455
                     /locus_tag="ONT67_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2355832.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT67_002105"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ONT67_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCR2355833.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ONT67_002106"
     CDS             14281..15654
                     /locus_tag="ONT67_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2355834.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT67_002107"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ONT67_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCR2355835.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ONT67_002108"
     CDS             16709..17149
                     /locus_tag="ONT67_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2355836.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3008 c         3795 g         4546 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tggg
//
LOCUS       DAJSBM010000057        17205 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJSBM010000057 DAJSBM010000000
VERSION     DAJSBM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.057"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(492..731)
                     /locus_tag="ONT67_002109"
     CDS             complement(492..731)
                     /locus_tag="ONT67_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355837.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1017..1331
                     /locus_tag="ONT67_002110"
     CDS             1017..1331
                     /locus_tag="ONT67_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355838.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1765..2131)
                     /gene="ssrA"
                     /locus_tag="ONT67_002111"
     tmRNA           complement(1765..2131)
                     /gene="ssrA"
                     /locus_tag="ONT67_002111"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2302..3075)
                     /locus_tag="ONT67_002112"
     CDS             complement(2302..3075)
                     /locus_tag="ONT67_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2355839.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3219..3569
                     /locus_tag="ONT67_002113"
     CDS             3219..3569
                     /locus_tag="ONT67_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCR2355840.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3550..4266
                     /locus_tag="ONT67_002114"
     CDS             3550..4266
                     /locus_tag="ONT67_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2355841.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4266..5714
                     /locus_tag="ONT67_002115"
     CDS             4266..5714
                     /locus_tag="ONT67_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2355842.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5784..6293)
                     /locus_tag="ONT67_002116"
     CDS             complement(5784..6293)
                     /locus_tag="ONT67_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355843.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6283..7008)
                     /locus_tag="ONT67_002117"
     CDS             complement(6283..7008)
                     /locus_tag="ONT67_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355844.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7231..9351)
                     /locus_tag="ONT67_002118"
     CDS             complement(7231..9351)
                     /locus_tag="ONT67_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCR2355845.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9581..10759
                     /locus_tag="ONT67_002119"
     CDS             9581..10759
                     /locus_tag="ONT67_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCR2355846.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10775..11533
                     /gene="aroD"
                     /locus_tag="ONT67_002120"
     CDS             10775..11533
                     /gene="aroD"
                     /locus_tag="ONT67_002120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR2355847.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11556..12041
                     /gene="ybaK"
                     /locus_tag="ONT67_002121"
     CDS             11556..12041
                     /gene="ybaK"
                     /locus_tag="ONT67_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCR2355848.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(12112..13365)
                     /locus_tag="ONT67_002122"
     CDS             complement(12112..13365)
                     /locus_tag="ONT67_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2355849.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13483..14832
                     /gene="gdhA"
                     /locus_tag="ONT67_002123"
     CDS             13483..14832
                     /gene="gdhA"
                     /locus_tag="ONT67_002123"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR2355850.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14944..16290
                     /locus_tag="ONT67_002124"
     CDS             14944..16290
                     /locus_tag="ONT67_002124"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR2355851.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16598..16984)
                     /locus_tag="ONT67_002125"
     CDS             complement(16598..16984)
                     /locus_tag="ONT67_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2355852.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(17033..>17205)
                     /locus_tag="ONT67_002126"
     CDS             complement(17033..>17205)
                     /locus_tag="ONT67_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2355853.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5637 a         3019 c         3419 g         5130 t
ORIGIN      
        1 cgtcgatcgc aatgaaatat tagaaaaatg cggaaaagta gtggcaaaat atactcacgt
       61 ttatgagttg gaaacaacag aatatctata taactatagc aacaaatatg aacatacttt
      121 agtttcataa agatttaaat aaagtgcctt caaaagcgag aagaccaagc ttttgaaggc
      181 actttattta gttggcagtg attgttcatg atttttgatt tattcaaaag gttcgcctag
      241 agaatctgtc tctgtaacga ggcctggtgg agaaactcga taaaaaccta tactgccaga
      301 actgtcctat tttcggatag acaaactagt agttttaaaa agatcatccg atccaatcat
      361 ttcaaaaaat gcaaaactga tatcaggatc atttagctca ggcttgttat cgtaaagcat
      421 ttgtaaaggt tgcatttagt taggagtaaa cgcgaaacta tcatcattat ttgctgaaga
      481 agatgaagaa gctaattcag agtttgtttg aaataaagga gacgtgctga cagtttcttt
      541 accctgtttt ttgcagaggt agaaactttt atatcggaag tttctaccgg agaatttttc
      601 tcatttgtag aaattgttga atcgatacta ttcgaaattt gcgtatctga aggtatgttc
      661 tttgatgcag aagtcccaca agcagtaagt aacaataaat ttgataaact gataaacaga
      721 attcttttca ttatgtacac actccttata gtatcgttta gcatacacct atgctcagta
      781 ttaggcaaaa tcaatgcaaa actatcaagt cttttatagt atctattagt cttagtaata
      841 catatatgga gagggtgata tcccagttca aaccagatac attggttacc atctgtatta
      901 acgattatgc ctctaaacgc tttttttatg aaaaaattta taagtgaaaa cgtaggtatg
      961 atattatcta gaggtaattt ttttgggtat ttaaatagtt gctaggagga tagcttttgg
     1021 atttttacag taattttatc ttaataatag caatattatt acttctaaat atttggtttt
     1081 ttgataagag tagaaatgca gggataggtt ttagaaccaa acgaagtacg tcttctgaaa
     1141 aaaagtgggt atattcacag actatttttt acggaggggt tatttcaatc agtttacttt
     1201 cttcaacact ttattctttt aatgttatag acgtttccat gtcaaatttt atatctatta
     1261 ttggaatcct tatttctgca attattacac aactacttct tgtgtttgaa gagaagtcaa
     1321 aaaataatta aagcaattat gccttttagt acaggaatta ttacttctta caaatgtata
     1381 gcgaatgtga agcctgctgt tttaattgtt ctagaaaaac ttttgagtga acaattattt
     1441 tatgttagga tgaattaatt tctctagagt ttataattaa aaaacttatt tgaaggaaca
     1501 tgcttaaacc ataattgtgt ttttccttcc atcagcataa aagttcacct caaaaagagt
     1561 gtacgaacaa aagttctttt tgtaaacctt aaaatagatt ttcctgaata cgtattgggg
     1621 atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa tgcaaaaaat tttgtatttc
     1681 tctacttttc tctccttgac caaaataaaa aaacgttgat ttaacaacgt ttttcataca
     1741 tgtttatctg tctcaaccat acatatatgg agacggcggg agtcgaaccc gcgtccaaac
     1801 acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct
     1861 tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg
     1921 gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg
     1981 agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag
     2041 cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct
     2101 cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt
     2161 catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc
     2221 attatttata taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag
     2281 aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc
     2341 gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt
     2401 gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc
     2461 aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc
     2521 cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt
     2581 tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat
     2641 atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa
     2701 gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct gatcgttata
     2761 gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga
     2821 agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt
     2881 gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc
     2941 tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc
     3001 gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc
     3061 acgataattt gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga
     3121 atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt
     3181 tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta
     3241 tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt
     3301 tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa
     3361 agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt
     3421 taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa
     3481 ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag
     3541 ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag
     3601 atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc
     3661 gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc
     3721 acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt
     3781 atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca
     3841 gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg
     3901 catcgccgta gtgagttggc agaagcaaga gaagaagcag cagtttcaga tgatcatttt
     3961 gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac
     4021 cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa
     4081 ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt
     4141 ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc
     4201 ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt
     4261 gcctaatgcg ttatttgtat cagcaactct tagctttttg cggaatggtc gcgttgatct
     4321 tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt
     4381 accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata
     4441 cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag
     4501 tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc
     4561 cgactaatac agctttactg aattttacct taacagctga tcaatggaaa aatctgagga
     4621 atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct
     4681 cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc
     4741 aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat
     4801 ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta
     4861 agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac
     4921 agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga
     4981 cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat
     5041 ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg
     5101 aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga
     5161 aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc
     5221 gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata
     5281 ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg
     5341 ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta
     5401 ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct
     5461 atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga
     5521 aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg
     5581 aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag
     5641 aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa
     5701 tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt
     5761 gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt
     5821 tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa
     5881 taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct
     5941 tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt
     6001 tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt
     6061 tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg
     6121 tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc
     6181 agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga
     6241 ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc
     6301 aggcctccta ctttgcaaat gatggataaa aaaagtgaaa gggaatagac taagaatgag
     6361 acagcatcca ctgagcccaa taagagtttg ggcaaatccc ttaagtatat ctgtgaaaaa
     6421 aagatcattt tggaaatcga tggtaaaaaa agtctgttga tccatcggaa ttgtaagtcc
     6481 gcttaccgaa gtcaagatat cacggatatc tggcagttca gtagtcttta agacaagaaa
     6541 attgatcctt tgaagaagcg cttcataaac tgactggaag cttacgatcc acaataaaac
     6601 caagatacaa caaagaaaga gaggaaagaa cagctgccag aaaataaaaa ggaataattt
     6661 ggttttagaa agaccagtca ggcgccaatt gatcaattcc tgcttgtttt gcctgactga
     6721 atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa aataaactga atatgccttt
     6781 tatataaaat cggctagcta attctaaagc cggaagaagc tctgtagatg cagaagcgcc
     6841 aataaatgaa agccgatctt ggacttgatt ggctaaagcc gattgcaaat ggtaaagagt
     6901 caggcatgct atcaataaaa aagcagaaaa gcttgaaaat aaacaataga gtattgtctg
     6961 ctttttgtaa taaattaatc gagcagtagc atagcggatt attttcatac ttttcacctc
     7021 tataaaaagt atagaaaatg aatatgaata aagtatgaaa ttcctttagt gaaatcacga
     7081 attaaaacca gaaaatattt tgtaaaaagc aagaggttgt gaagaagctg ccctattcta
     7141 ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa agaggagcag cgacaataat
     7201 tttgtcgctg ctccttttaa atttattttg tcattcggaa gaacttgtgg tcgttccgtg
     7261 aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccgt tgtgttcact
     7321 aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt tctctaagcc
     7381 attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg tatagaaccg
     7441 aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa ctttttcttt
     7501 cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt caccagtttc
     7561 gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat caccatcacg
     7621 gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact gaatataatt
     7681 ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt gtccgccgta
     7741 agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag ggacacgctg
     7801 catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta attcagctag
     7861 atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga tgactgaatt
     7921 atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg tatctaaata
     7981 attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta acgggaatcc
     8041 tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa tgaattttga
     8101 ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa atggtttatc
     8161 atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag aatcaaagaa
     8221 ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac cagcgttccc
     8281 atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg cagcttggaa
     8341 agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa acaatgaatt
     8401 ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga aaaagttgtc
     8461 aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa cttcatgctg
     8521 gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc tttctaaatg
     8581 gatataaatc acattttttc ctttagcgag cccgaacaaa tcgtcatttg gtgctgcata
     8641 atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt ctgcccgcac
     8701 ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca agccaagata
     8761 tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat cagcttccgc
     8821 cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa aagccattct
     8881 tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa cagtcaagat
     8941 aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg cactttctcc
     9001 cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga agtccgtgaa
     9061 ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat aaatgaccat
     9121 catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa gtaataacaa
     9181 tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt cgattccgag
     9241 atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacga ctagtaaaga
     9301 aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca agataatctt
     9361 cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag ctcacttatt
     9421 ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt ttatgctgtt
     9481 tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt cctatgtttt
     9541 ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa aagtaaaaaa
     9601 aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag accttgctca
     9661 tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc aatttatcgc
     9721 aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc agcagaacat
     9781 cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa aaagaacttt
     9841 ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag gagacggaat
     9901 cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt ataacgaaac
     9961 gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc cggaaatcgt
    10021 aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc agttcattta
    10081 cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat ttgccgttta
    10141 tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac ggggatcatt
    10201 agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata ccggcgcctt
    10261 ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc tagcaaaacg
    10321 cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg atcagcaaaa
    10381 aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac aatttttata
    10441 tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag tctttcgcgt
    10501 agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag aagacgggat
    10561 acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa caatgattga
    10621 agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc gtctgccaaa
    10681 agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt ttgtttatca
    10741 aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg gattgaatat
    10801 aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag aggaagtatt
    10861 acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc gagtcgatca
    10921 ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta tccatgagat
    10981 gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg gagaacaaga
    11041 catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag gattagtaga
    11101 tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga tagaggaaat
    11161 caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa caccagcaga
    11221 ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata tcggaaaact
    11281 ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga ctagacgggc
    11341 aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg gtaaaatttc
    11401 tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa gcagcgattc
    11461 tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc aattattaaa
    11521 tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa atagtaaaaa
    11581 cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac gaatatgagt
    11641 gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa agccaatctc
    11701 gtatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt gctgtgatac
    11761 cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat aaaaaagtag
    11821 aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt ggctgctcgc
    11881 ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa agctatgaac
    11941 aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct caagatatct
    12001 gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt ataaaagaaa
    12061 gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac attatgctct
    12121 ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga tattaaagcc
    12181 gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat caatgatttt
    12241 actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag ttacaaatga
    12301 tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata gttgaacaaa
    12361 atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt caagccgctc
    12421 aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga ccaagttctt
    12481 tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg tttgtgtagg
    12541 gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt tattgacaaa
    12601 acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa gtaaatccat
    12661 cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga tgtcttgtaa
    12721 agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca agggggactc
    12781 tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt acccatctcc
    12841 agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg caggagcatg
    12901 gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga tcaatttctc
    12961 tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga gcaatgcttc
    13021 gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct cttcttttaa
    13081 atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt gcgcaaacgt
    13141 actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa caggacgatt
    13201 gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg cttttttgtt
    13261 tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg ccatcatccc
    13321 gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct cacctcaagg
    13381 aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat gttttgagcc aaaagagaaa
    13441 atgagctaaa atgagggaag ataagcaaag gagcgtggaa cgatgacaaa cgcaacagaa
    13501 tatgttcaag caattcaaga aaagctacat caaaaggatc aagggcaaga agaattttta
    13561 caagcaatcg atgaattcat gcccacggtt atgtcttttt tggacactca tcccgaatat
    13621 atcgaaaaga atatcttaga acttttaacg gaaccagaaa gagttattca atttcgagtg
    13681 ccttggcaag atgatgaagg aaactggcga gtaaatagag ggtaccgtat ccaatacaac
    13741 tcagctatcg gcccttataa aggtggattg cgcttccatc ctagtgtcaa tctaagtatc
    13801 ttgaaatttt tggcttttga acagattttc aagaatagtt taaccggtct gccaatcggt
    13861 ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat ctgataatga gatcatgcgt
    13921 ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg gcccatcgat tgatgttcct
    13981 gcgggagata tcggtgtagg agctagagaa atcggctatt tgttcggcga atataaacga
    14041 ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt tggatttttg gggaagtaag
    14101 atacgtacgg aagctactgg ttatggatta gtgtattatg tcaaacatct attgaacgaa
    14161 gaaaaagatt catttaatca aaaaacagtt tttgtttccg gaagcgggaa tgtagccatt
    14221 tatgcaatcg aaaaagtgca agagctaggc gggaaagttg ttacatgttc agattctagc
    14281 gggtacatct atgatccaga aggaatagat gtgaaactat tgaaagaagt caaagaaatc
    14341 aatagacaac gtttgacgga atacgcaaac caacgtcctt ctgctattta tcatgctgga
    14401 gaatctgttt ggacattgaa agaaaaagcg gatatcgctt tgccatgtgc tactcaaaat
    14461 gaaatagacg aaagcttggc cgaaatctta gtagaaaatg ggatcaaaat cgtagcagaa
    14521 ggcgctaata tgccatgtac agtaaaagct gtttctgttc ttcatgaagc aggcgtgtgg
    14581 tattgtccag gaaaagctgc taatgctgga ggagtagcag tatctgcttt agaaatgagt
    14641 cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg atgagcagct ggatcaaatc
    14701 atggaaacga tttaccatac gtgtcgccaa acagcagccg agtacggcga cgaaaaaaat
    14761 ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg ccaaagcaat ggcagcagaa
    14821 ggtttagttt gaaaaatgaa actttcttgg aaatattgtc agcattttgt ttatttgctt
    14881 gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag taaacctata aggaggagaa
    14941 caaatggcac acattcattt tgactattca aaagtagcac cgtttgtcaa cgaacaagag
    15001 ttagggtaca tgcaaagcca agtaacagca gcccacaacg aattacggga aggtactggc
    15061 gcaggaagcg acttccgcgg ctggatcgat ttaccaacga actatgacaa agaagaattt
    15121 gcacgtatca aagaagcagc taaaaaaatc caatctgatt ctgaagtatt agttgttatc
    15181 ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg acttcttgaa tcacacattc
    15241 ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa tcttctttgc cggaaattca
    15301 atcagctcaa catatattgc tgacttgatc gaagtaatcg gtgatcgcga cttttcagta
    15361 aacgttattt caaaatctgg aacaactact gaaccagcga ttgcgttccg tgtattcaaa
    15421 gagcttcttg ttaagaaata tggacaagaa gaagcaaaca aacgtatcta cgctacaaca
    15481 gataaagcaa aaggtgcagt taaagtagaa gcagatgcag aaggttggga aactttcgtg
    15541 attcctgatg acatcggcgg acgtttctct gtattaactg ctgttggttt gttaccaatc
    15601 gctgttagtg gtgcagatat cgatgcattg atgcaaggtg ccgctgatgc aagcaaagca
    15661 tactcaagcg acaaactaga agaaaacgaa gcttaccaat acgcagcaat gcgcaacatt
    15721 ctttaccgta aaggcaaagt aactgagttg ttgatcaact atgaaccagg aatgcaatac
    15781 ttctcagaat ggtggaaaca attgtatggt gaatctgaag gaaaagacca aaaaggcatc
    15841 tatccttcaa gcgcaaactt ctcaactgat ttgcactctt taggacaatt catccaagaa
    15901 ggacgtcgca atatttttga aacagttgtg aaggttgaaa aacctcgtaa atcaatcaca
    15961 attcctgaac aagcagaaga tttagacggt ctaggctact tgcaaggaaa agaagtggac
    16021 ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc atacagacgg tgacgtgcct
    16081 aacttgctag tcaaaattcc agaaatggat gcttatacat tgggttatac aatgtacttc
    16141 tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa tcaacccatt cgaccaacca
    16201 ggtgtagaag cttacaagaa aaacatgttc gcacttctag gcaaaccagg ctttgaagat
    16261 cttgctaaag aattgaacga acgcctgtaa gataagggtt tttcgctaga tttaaataat
    16321 cagtgataga aacatgttcg ctttaaaatg aaaactggta cccatttagg tactcaaaca
    16381 tactgcatcg tttattcaga ataaggggct gtgacaagac gtttgtcaca gtcccttgtt
    16441 tgtatagaaa tcgattgatt gccttgctct ttagtttgtt ttaggacttt aagaacagtg
    16501 aggctgactt cgaaggttaa ttctgttttt ttgttttcta gaatcagtaa gcaaaaaagc
    16561 actcacttcg agtgcttttt tgcgatatat tcttagctta agataggcct aatttttctt
    16621 ttacagatga gggtacctta tcgctggtaa ttactttttt ctctatgatc cgcttttcgc
    16681 ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga accattggca gagtcaccag
    16741 aagtaatgac atcgaaatct aactgtcttt tttcaccgtt ttcagcataa gcatctacgt
    16801 tgtatttaaa acctcttcca attacttccc cattatctgc tttgatatca gtttctgcag
    16861 gcattggagc tttgactacc gcataataat ctttacctac ataagtagac tgatagtagt
    16921 cccaaccttt ccatcctata aaagcaatca atatgataac gatcaaccct aaaatttttt
    16981 tcatgaaaga tctccttttt tatttttatt atacccgtgg tgctagttaa ttttaatatc
    17041 ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat agaacactgt
    17101 aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt gagttgaaat
    17161 tttgacaacc taatttttct aaggaagaaa aaacagtctc aactg
//
LOCUS       DAJSBM010000058        16937 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJSBM010000058 DAJSBM010000000
VERSION     DAJSBM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.058"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="ONT67_002127"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="ONT67_002127"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR2355854.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="ONT67_002128"
     CDS             1281..1727
                     /locus_tag="ONT67_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCR2355855.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="ONT67_002129"
     CDS             2000..3352
                     /locus_tag="ONT67_002129"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCR2355856.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="ONT67_002130"
     CDS             3523..3810
                     /locus_tag="ONT67_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355857.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="ONT67_002131"
     CDS             4036..4479
                     /locus_tag="ONT67_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2355858.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="ONT67_002132"
     CDS             complement(4485..5669)
                     /locus_tag="ONT67_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCR2355859.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="ONT67_002133"
     CDS             5937..6635
                     /locus_tag="ONT67_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCR2355860.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="ONT67_002134"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="ONT67_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR2355861.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="ONT67_002135"
     CDS             complement(7445..7978)
                     /locus_tag="ONT67_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355862.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="ONT67_002136"
     CDS             complement(8275..10656)
                     /locus_tag="ONT67_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCR2355863.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="ONT67_002137"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="ONT67_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCR2355864.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="ONT67_002138"
     CDS             11357..11896
                     /locus_tag="ONT67_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCR2355865.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="ONT67_002139"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="ONT67_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCR2355866.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="ONT67_002140"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ONT67_002140"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="ONT67_002141"
     CDS             13003..14169
                     /locus_tag="ONT67_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCR2355867.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="ONT67_002142"
     CDS             14215..15711
                     /locus_tag="ONT67_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCR2355868.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="ONT67_002143"
     CDS             15792..16475
                     /locus_tag="ONT67_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2355869.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         5744 a         2892 c         3499 g         4802 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggt
//
LOCUS       DAJSBM010000059        16369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJSBM010000059 DAJSBM010000000
VERSION     DAJSBM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.059"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="ONT67_002144"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="ONT67_002144"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCR2355870.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="ONT67_002145"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="ONT67_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCR2355871.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="ONT67_002146"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="ONT67_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCR2355872.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="ONT67_002147"
     CDS             2857..3732
                     /locus_tag="ONT67_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCR2355873.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT67_002148"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="ONT67_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR2355874.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="ONT67_002149"
     CDS             4629..5063
                     /locus_tag="ONT67_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR2355875.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="ONT67_002150"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="ONT67_002150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR2355876.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="ONT67_002151"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="ONT67_002151"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR2355877.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="ONT67_002152"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="ONT67_002152"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR2355878.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="ONT67_002153"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="ONT67_002153"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCR2355879.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="ONT67_002154"
     CDS             13334..14260
                     /locus_tag="ONT67_002154"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCR2355880.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="ONT67_002155"
     CDS             14442..15335
                     /locus_tag="ONT67_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCR2355881.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="ONT67_002156"
     CDS             15469..15594
                     /locus_tag="ONT67_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355882.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="ONT67_002157"
     CDS             complement(15728..16360)
                     /locus_tag="ONT67_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355883.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5812 a         2791 c         3492 g         4274 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcc
//
LOCUS       DAJSBM010000060        15936 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJSBM010000060 DAJSBM010000000
VERSION     DAJSBM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.060"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..1475
                     /locus_tag="ONT67_002158"
     CDS             138..1475
                     /locus_tag="ONT67_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCR2355884.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="ONT67_002159"
     CDS             1523..3652
                     /locus_tag="ONT67_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCR2355885.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="ONT67_002160"
     CDS             3627..4316
                     /locus_tag="ONT67_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355886.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="ONT67_002161"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="ONT67_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5713)
                     /locus_tag="ONT67_002162"
     CDS             complement(5546..5713)
                     /locus_tag="ONT67_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355887.1"
                     /translation="MYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKESEAFAKRIGG
                     RVKPIQEVRPD"
     gene            complement(5792..6232)
                     /locus_tag="ONT67_002163"
     CDS             complement(5792..6232)
                     /locus_tag="ONT67_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCR2355888.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6236..7081)
                     /locus_tag="ONT67_002164"
     CDS             complement(6236..7081)
                     /locus_tag="ONT67_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCR2355889.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7230..8648
                     /gene="pepV"
                     /locus_tag="ONT67_002165"
     CDS             7230..8648
                     /gene="pepV"
                     /locus_tag="ONT67_002165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCR2355890.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8705..9652)
                     /locus_tag="ONT67_002166"
     CDS             complement(8705..9652)
                     /locus_tag="ONT67_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2355891.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9875..10225)
                     /locus_tag="ONT67_002167"
     CDS             complement(9875..10225)
                     /locus_tag="ONT67_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCR2355892.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10425..11504
                     /gene="pepA"
                     /locus_tag="ONT67_002168"
     CDS             10425..11504
                     /gene="pepA"
                     /locus_tag="ONT67_002168"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCR2355893.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11648..11968
                     /locus_tag="ONT67_002169"
     CDS             11648..11968
                     /locus_tag="ONT67_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2355894.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11990..12454
                     /locus_tag="ONT67_002170"
     CDS             11990..12454
                     /locus_tag="ONT67_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2355895.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12659..13264
                     /locus_tag="ONT67_002171"
     CDS             12659..13264
                     /locus_tag="ONT67_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCR2355896.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13303..14592)
                     /locus_tag="ONT67_002172"
     CDS             complement(13303..14592)
                     /locus_tag="ONT67_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCR2355897.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT67_002173"
     CDS             15020..15499
                     /gene="rlmH"
                     /locus_tag="ONT67_002173"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR2355898.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15702..15936
                     /locus_tag="ONT67_002174"
                     /pseudo
     CDS             15702..15936
                     /locus_tag="ONT67_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5023 a         2935 c         3251 g         4727 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catatgggga tcacctgctt ttttcttttc attatacgaa ctactcgaca
     5761 caatacaaga aaaatgctta gacggtcttt attaagaatt tacatcatcc aaagaagcgt
     5821 acgcttggaa taactgatct aactccggat tgactttgct cagcttcact aatcgttgcg
     5881 aatcagaaag taaaaaacag tgtttactac tgccagtagt ttttaatttt ttgtcttcag
     5941 ttcccacaat ttcgtagcta aaatccaacc gtgtccccgt atacttttgg atagttggat
     6001 ggatgctcac tttttctccg taatggatca tttctttata gctgcaattt acttccagaa
     6061 cggggatgat gatcccagat tcttcgattt ttttataggg aaaccctaga tgtaccaata
     6121 aggctacccg agcctcttca aaccagcgga tataattgct gtgatgaata atccccattt
     6181 gatctgtttc ataataaaaa ggttctcgca cataacctgt aaatttctcc atgactcatg
     6241 cctcgttttc cattgatttt ttacaaaaat aattcatcca tttcggaagt gcttcgctga
     6301 tttgtgtcgg caaaacaata tattgttctt ttgccagctg gtctccaatg aaactatgaa
     6361 gataaatagc tgctgcaatc gtttggtcat tttgaggaaa ctgggctaaa aatcctgtga
     6421 tcatcccagc taaagtgtca cccatgccgc ccgttgccat agcagcagaa cctaaaggat
     6481 tggtgtagaa gtgctggtga ccataaattt ttgtctcatg acttttcaaa acgagcacag
     6541 caccgatttc ctgttgcttc tttttgtttt ctttatcccg ttgttcttgt atcggtagag
     6601 aactcaaccg ttgccactcc atttgatgag gtgtaagtac agtttgttcg ggatagggta
     6661 gcaaaagatg attagcagca agtaaactta aagcagagcc atcaatcacc agccattgtt
     6721 cttttgattg gttttctagt acttgtttga aacgttccaa actcttagaa gaagtaccca
     6781 atccgggacc aatcaagagt acatctgctt gctttattac ggaaagaaac tggtcagtat
     6841 ccgtccaaac tacaatcatt gcttctggca gatgactgtg aagagcactc cgattttttt
     6901 catcagttac tacagtcgtg agacccgctc cgctgtgtac ggcagctaaa gcactcataa
     6961 tgattgctcc gccatattgt tcattgccgc caatcaaaac gatacgtcca aatgttcctt
     7021 tatgtgaaat ttttggacga ggtgtgatga ctcttttcac tagttgttcg tttatcagca
     7081 tctaaaaacc tccttttact cattatagcg tatatttttt ttagagaaaa acagataaga
     7141 aaaggaattg tcagattttt tgaactattt ttttctgtaa tttgtggtat catgaacacg
     7201 aagacttaaa taagtggagg attaacttta tgacaattga ttggcaaaaa gaagtagaag
     7261 cacgtaaaga agacttgctt gaagacttga aaaatctttt acgtgtcaac agcgagcgcg
     7321 atgactcaaa agtaacacca gatgcaccat ttggacctgg acctcgcgac gcattgaaac
     7381 atatgttagc ttatggcgaa cgtgatggat ttaaagtaaa aaatgtcgat aactatgcag
     7441 ggcatattga tcttggcgaa ggagacgaaa cgctagggat atttggacac atggatgtcg
     7501 ttcctgcagg agatggctgg gacacagatc cttatgaacc agtaatcaag gatggaaaga
     7561 tctttgcacg cggatcaagc gatgacaaag gtccaagtat ggcagcttac tatgcgatga
     7621 agatcattaa agaacttgac ttgaaactat caaaaaaagt tcgcttcgta gtcggcagcg
     7681 atgaagaaag cggctggggc gatatggctt attatttcga acatgaagaa gaaccagatt
     7741 ttggtttctc accagatgct gaattcccaa tcatcaatgg tgaaaaagga aatgtttctt
     7801 tagctttacg cttcaaaggc gacaatgcag gagattatgt cttgaaatct tttgtttccg
     7861 gacttcgcga aaatatggtg ccaggaacag caactgcagc acttcaagta ccaagtgcag
     7921 atgcagctat cgcgatggaa gaagcatttt accaattcat cgaagcaaat ccagtaagcg
     7981 gaacgattga agcggacaat acgtatgtaa aaatcgaatt agttggtaaa ggcgcacatg
     8041 gtgcaagccc acaatctggt atcaatgcag gttctttctt ggcattgttc ttagataact
     8101 atgaattcct aggatctgct aaacaattta tccatgtagc agctgcttat gtccatgaag
     8161 atttttatgg agaaaaacta ggagtcgctt atgaagatga aaaaatgggc aaattgacaa
     8221 tgaatgctgg actatttgct tttgaagaaa atggtacaga ggaagcgaac ttcatcaata
     8281 tgaatttccg ttttccaaaa ggtgtaacag tagacggatt gcaaagtgac atcgaacaaa
     8341 cagtggggca agaaggcgca acagttactc gaggtgctcg cgtaatggaa ccacattacg
     8401 taccaatgga tgatccattg gtggctacat tattgcaagt ctatgaagac cacactggag
     8461 aaaaaggcta cgaacaaatc atcggtggcg gaacttacgg acgtttgttg aaacgtggcg
     8521 ttgcttatgg agcgatgttc ccaggatata ctgatacaat gcatcaagca aacgaattta
     8581 tgagtcttga tgacttgttc agagcaacag caatctatgc agatgccatc tatcgtttgg
     8641 cgaaataatt aattaaaaag aggatcaaga attgcaaatt atttttgcag tcttgatcct
     8701 ttttttactt tctactgtgg tattcatgat aaacatgata tagattcaat tcatattctt
     8761 tcttagcgtt cgtgacgact gtatccaaaa tcaaccctgt tactaatgaa agaagtgcta
     8821 aagtcatcaa tccagtactt aaaatcgcag aagggatgcg tgtaataaag cttgtcagca
     8881 tatattcgcg aatcacagga atccccgtga ccaatccaaa taaaaagaag aagatggtcc
     8941 aaatcccaaa gaaaagcaaa ggcttataat ctttaaacat tttaataatc atcatgatca
     9001 ctttgaatcc gtcagagaat gtattcaatt ttgattcgct gccttcagga cgatcacgat
     9061 aatcgattgg gatttcaacg aacttgaatt ttttatctaa agcatggatc gtcaattccg
     9121 tttcgatttg aaaaccagaa ctcataatag gaaaactttt gacaaagata cgattgaatc
     9181 ctcgatagcc ggtcatgaca tcaagaattt ttgttttata cattctagta attgtatttc
     9241 taacaaggtt atttccaaaa tcgtggaaag gtctcttatt ctcatcaaag taggttccat
     9301 ttgataggcg atcaccaatg accatatctg cttcaccaga acgtaatttc tcgagcaaac
     9361 catgtacagc ttctgcagga taagtatcat cgccatctac cattaaataa tagtcggcat
     9421 cgatatcaaa gaacatttgg cggatcacat ttccttttcc ttgacgaggt tcttttttta
     9481 cgattgctcc accttgaacg gccaattcgt atgttcgatc ggtcgaatta ttatcataga
     9541 cataaatatc tgcttctggt aattctcgtt taaagtctgc aatgactgtt gaaattgttg
     9601 cttcctcatt gtaacaagga atcagaacag caattttttc ttgttgttcc atttttcact
     9661 ctccttaact atagaaaaca aaagctaaat taaaaatacc tcataatcaa aggaaatgct
     9721 agtttttccc gctaaatatc atttatttaa aaagaaaata ctggaatata tgtttggaaa
     9781 gctctatcta aaaaaccacc cactacaaaa gaaggacatg atacctctct tttgaatagt
     9841 gggtgcgtat tcgttctctc acttttttga cgtattaatc tgttgcacct ttttttcttg
     9901 aaatttctaa tacggttcct gtatgagcat ctgctaaaaa ttcataataa acagtttcgc
     9961 catcttctat tcgagaaaga cctccccgat aagcactcga ccgaacggcg aatttcctaa
    10021 taggttcctt ttcaaaagag atccaagaac cttcgattgc accttctttt agaaaagatg
    10081 acttgatatt ttcaagtact tggtctgctg acaatacttt tttgcgatga taccaaatcg
    10141 ttgaagctac cccgctcaat aatcctagac cgattccaac tgctaacccg cctttaaaat
    10201 agcttagttc gctttcttca tacatgtgga catacctcct tcttctcact tgttactcct
    10261 tgtgcttatt ttaccacact ctcatatttt ctttgaaata aataaccgct accagaaaaa
    10321 ataagctata ataaatacga tcggtctgca gaaaaaatca tattttatcc gcagatcaat
    10381 ataagtctga gaggagttta gtctgaaatt tagacgaata gcatatggaa gaaaaaacat
    10441 ttcaacgaat caaagagtta acagaattac aaggaacaag cggttttgaa caagatgttc
    10501 gcgcttatat ggaaagtcat atggaaccgc tggtagatga gttacagtta gatggtttgg
    10561 gcggcatttt tggtctgcgc catcatgaag aagcggatgc accccgtgta atggtcgcag
    10621 cacatatgga tgaagtcgga ttcatgctga cacaaatcca agaaaacggg ctttttaaag
    10681 tcgtacctct aggcggctgg aatccttacg ttgtttctgc tcagcgtttt acacttaaaa
    10741 cctctactgg taagaattat ccatgtattt cttcttccgt tcccccacat ttattgcgag
    10801 gaacaagcgg acaaaaatca gtagaagtaa ccgatatttt gttcgatgct gggtttgaat
    10861 caagagaaga ggcaatgagt tacggtgtcc ttccaggaga cacaatcgtt ccttacgctg
    10921 aaacgatcaa aacagccaac ggaaagaata ttatcagtaa atcatgggac aaccgctatg
    10981 gctgtacaat ggtattagaa gctttagaag cgttgcaaaa tgaaacatta ggtcatacat
    11041 tgattgcagg cgcgaatgtc caggaagaag taggcttgcg tggttctaaa gcttctgtca
    11101 ataaattcaa gccagatctg ttctttgcag tagactgctc tgcagcagat gatacagtga
    11161 cgaaaaatgg cacattcgga catttaggag aaggtacatt gatgcgtatc caagatccag
    11221 gtttgatcat gcttcctcgt ttgcgcgagt acttgctgga tatcgctgaa acaaacaata
    11281 tcccttatca atactttgtt tcaaaaggcg gtacagatgc aggtgctgct catacacaaa
    11341 acgaaggaat cccaagtaca gtgattggcg tagtgggacg ttatatccat acgcatcaaa
    11401 cgatgttcag tattcgcgat tttgaagctg cacgagaaat gttgattcaa acactgaaag
    11461 gtttggataa atcaacagta aatacgatcg tatacggaaa ataatgcgat agtgaaacta
    11521 taaaatctgg caaaagattg ctttttgcca gatttttttt ataaaaagag gatcaatgtt
    11581 tgttagttcg aaagaaaggt gtataattta attgaaaaat acataaacag aaagtagagg
    11641 acaaagaatg attatcccaa atagtttaga agaattagca ggctacgtag aaactggaaa
    11701 gagtgtattt ttctttactg ccggctggtg cggagactgc cgttttatca aaccgcaaat
    11761 gcctgaaatt gaaaatgact tttctgattg gcaatttatt gaagtagacc gtgacaagta
    11821 catagatgta gccgctgaat ggaacatttt tggtatccca agttttgtcg tgatccaaga
    11881 cggaaaagaa ttaggtcgtc tagtgaataa agatcgtaaa acaaaacagg aaatcgaatc
    11941 gttcctagaa agtgtaacaa aaggctgaca tctgttagga ggggtcaaga tgaatcaaga
    12001 atataagaat attttagtag gtatcgacgg tagtgaacaa gcacttgctg ctttcaaaaa
    12061 agcggtagaa gtggctcgcc gtaataatgg gaaagtatat gtagcaaacg taatcgatca
    12121 gcaattttat aactttatgg gctatgcacc tattgatcaa aatctagtcg atcagcaaac
    12181 tgaaacagcc aaggaaatga tcgaagaatg taaagaatat gggaaatctg ttgattacac
    12241 aaatatagaa ggaattattg catatggttc tgttcgtgaa tccatggcag tcaaattgcc
    12301 aaaaaaatat gagatcgatc taatcatggt tgggcagtca ggtctaaatg cagtcgaacg
    12361 ctttatgaca ggaagtgtag ccagctacgt gatacgtcat tcaccttgtg atgtcttgat
    12421 cgtttctgac gaagaagaac agccaagtaa ttaagcaagt ggaacttgta cgaccttttt
    12481 aatcaattaa ctaaaacagt aaagggactg tgacaaaagt cttgtcacag tcccttgtta
    12541 cgaataaatg gtgttccaga agaggcttct tcggaaataa agcatgaaaa aaatttcatg
    12601 cagtgaatgc aattttttgt taagataaat ctcagcaaag gataatggag gaaagaacat
    12661 gatttttgct tataacaaag cacacgtcgg agacaccttg atggttatta tagccgacga
    12721 taaaggaaca gaaaacactg ttgaacgcaa atggaatgtt gctcggatca aagatgaaca
    12781 aggtcaagtt gttgcttgga actttttcca tatctccgat catctaacaa tcgaagggaa
    12841 cggccaagta agcttaacag atgaggaatt agcgaaattg aatcgattga tcaaagaagc
    12901 aggattcgaa gaacagcttg aagctgatca tgaaccgaag atcgttgtcg ggtacgtaaa
    12961 aacatgcgtc ccacatcctg attctgacca tttatcgatt actgagacag aagtagacaa
    13021 tggtacagta ttgcaaatcg tttgcggtgc acccaatatc gaagctggac aaaaagttgt
    13081 cgttgcaaaa ccaggagcta tgatgccaga tggaatgatg atctggccag gaactttgcg
    13141 aggggtagat agctatggaa tgatctgttc cgctcgtgaa ttgcatttgc caaatgctcc
    13201 agcaaaaaaa ggaatcttag tattgcctga ggatgccgta gtcggagaag catttccaaa
    13261 ataaagaggt tgtgacaagt ctttgtcaca acctcttttt tattaatttt cactattttg
    13321 attttgtttt aatgccgatt gatcgatcgt caattcaaca gtagttgttt tttctttcga
    13381 gccttcatag taagtgattt tgacagaatc tcccactttt ttcttataca aagcagattg
    13441 aagttctaca ccagaagaaa cttctttgtc atcgatctta gtaatgacgt cgtattgttt
    13501 caatccagct ttttcagccg gtgtagctgt ttggacattt gtcacgatga caccgttagt
    13561 tacagatgaa ggaatcttca agatttgttc ttgttgctga gtagaaacgg cagataagtc
    13621 gaccattgtg atacctagag ctggacgtgt cacttttccg tctttttcca gttgattgat
    13681 gatattcact acatcgttac ttggaatagc aaatcccatt ccttctacgc ttacattaga
    13741 agtagattca gaagtacttg cgatcttact tgagttgata ccgataactt gtccttcgat
    13801 attaactaaa ggaccaccag aatttccagg gttgattgct gcatctgttt ggatcgcatt
    13861 gatattgact gtttcgttcg attcattcgt acttgtcact tgtcggttca aggaagaaat
    13921 gattcctgaa gtgacagaat ttgcatattc agatcctagt ggggaaccaa tcgcaatcgc
    13981 aggctcacca accttcagag cacttgagtc accaaaagag gcaactgttt cgactttgtc
    14041 tgaattgatc ttcaacaccg ctaaatctga ataagcatct gttccaacta attcagcttt
    14101 taccttcgta ccgtctttca tcaagacttc aagaccttgc tggccatcta ctacgtgatt
    14161 attcgtgact acataagcag tattcccgtc ttttttgtag atcacgccgc ttccttcact
    14221 ataagcttcc aaattactat catctgagct gctactttct tcttgctgcc cgaatagtcc
    14281 gccaaaaccg cttgattggt tttgactttg taagttgata acagaaacga cagcgtcttg
    14341 aactttatct accgctttgg tgatatcaga atctacattg actttgacat tttccacaac
    14401 tgtttcaccg gcagaattct gattgccgct tgtagcagta gaactattgt tgccagtgcc
    14461 catcgctgca taaaaaatgc cagctgtcag tagtcctcca acgatccctc caacaagccc
    14521 cagacctaac ttacgccaga ggctattatt tttacttttt ttcatctttg gtgtcacatt
    14581 ttttctatcc atgtgttcct cctccaatta cattcattta tctactgtta gtatagtctt
    14641 atttgttgac tttagtctac tttgagactg tgaacttttt atgaaaaact ttaggaaaaa
    14701 tcattgtttt cagatgaaaa tatttgattt tctttttcca cagatactgt gtaaaactac
    14761 gcggatagga ttggaaaaat aagggataaa acatatccac agcctgtgta taaagtggat
    14821 aaattcttat aaaacgtatg tttgcctaat gaatgtgatg aaataaggta aaataacact
    14881 gttttctgtg gataaacctg tggatgttgt gcataactgt tggggaaatc tttttaacaa
    14941 aaatagccaa aataaacata tagacgaaaa aattgattat aataaacaaa gtgagcatga
    15001 agaccaaagg agagaaaaca tgaatatcaa aattatctca gtaggaaaat taaaagaaaa
    15061 atacctgatc caaggtatca atgagtatgt caaacggctg aatgcctatg caaaaatcga
    15121 attgatcgaa gttcctgacg aaaaagcccc ggagaattta agcgaagcgc aaatgagaca
    15181 agtaaaagaa aaagaagggg aacggatctt agcgaaaatc aaagaacaag aatacgtcta
    15241 tgccttagca attgaaggga aaaatcctac aagtgaatct tttgctaaac aaatcgacca
    15301 attaggtatc caaggaaaaa gccaccttgt ttttgttatc ggcggctctt taggactcag
    15361 cgaagccgtc atgaaaagaa gcaatgctca gatctctttt ggaaaaatga cgtatccaca
    15421 tcaattgatg cgtctgatcc ttgtcgagca aatctaccgt gccttccgga tcaatgcggg
    15481 ggctccatac cacaaataat gatatttttt aagagaaaaa attatcccta ttttaaataa
    15541 tagcaatagg tagtggtagg gagaaattta taaataactt ttttatttgc tttggtagcc
    15601 aagtaagttg caaaagaaaa atagatactg ttaaaaagat aatgtagaca tagaaacaaa
    15661 tacgtaaaat tttagttaga ataatggcaa ggagaaaaaa catgatagat gaaaaaaagt
    15721 gtttaaaaaa tctattagag gatatggatg atattgaaga aaactatcta aatggactgg
    15781 gaaaacaaat atatttcaga tacttaagct atctaaaaat gaaataagac atagtaattt
    15841 tttagctttt ttgtttgctc caaatgaaac ccacaattta tctgatgagt tttttaaaat
    15901 gtttttgaaa aggtatatag attctaatga tgatac
//
LOCUS       DAJSBM010000061        15026 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJSBM010000061 DAJSBM010000000
VERSION     DAJSBM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.061"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            74..583
                     /locus_tag="ONT67_002175"
     CDS             74..583
                     /locus_tag="ONT67_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2355899.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            676..1838
                     /locus_tag="ONT67_002176"
     CDS             join(676..952,952..1838)
                     /locus_tag="ONT67_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2355900.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2118..3098
                     /locus_tag="ONT67_002177"
     CDS             2118..3098
                     /locus_tag="ONT67_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355901.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3220..4638
                     /locus_tag="ONT67_002178"
     CDS             3220..4638
                     /locus_tag="ONT67_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HCR2355902.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4709..6178
                     /locus_tag="ONT67_002179"
     CDS             4709..6178
                     /locus_tag="ONT67_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2355903.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6188..6655
                     /locus_tag="ONT67_002180"
     CDS             6188..6655
                     /locus_tag="ONT67_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2355904.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6691..7563
                     /locus_tag="ONT67_002181"
     CDS             6691..7563
                     /locus_tag="ONT67_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2355905.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7644..10178
                     /locus_tag="ONT67_002182"
     CDS             7644..10178
                     /locus_tag="ONT67_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2355906.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10334..11107
                     /locus_tag="ONT67_002183"
     CDS             10334..11107
                     /locus_tag="ONT67_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2355907.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11100..11891
                     /locus_tag="ONT67_002184"
     CDS             11100..11891
                     /locus_tag="ONT67_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2355908.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11901..12392
                     /locus_tag="ONT67_002185"
     CDS             11901..12392
                     /locus_tag="ONT67_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2355909.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12410..12820
                     /locus_tag="ONT67_002186"
     CDS             12410..12820
                     /locus_tag="ONT67_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR2355910.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12824..14224
                     /locus_tag="ONT67_002187"
     CDS             12824..14224
                     /locus_tag="ONT67_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HCR2355911.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14247..14621
                     /locus_tag="ONT67_002188"
     CDS             14247..14621
                     /locus_tag="ONT67_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2355912.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5225 a         2108 c         3018 g         4675 t
ORIGIN      
        1 taaatttgat aaatagaaat tagtatagcc ctaaacaaat tatttgtgtt ttattgttta
       61 gggctatact atattggtac aaggaataga aagaggtgtt tatgtggttg ataaaattca
      121 gtttaatcaa gttatagaag aattcatcaa agtaaataat ttaatgaccc atattcaaaa
      181 acgaccaatc agaataacta atgatctaaa actcagtacg agtatgattc atttgattga
      241 tgtgattgga aattatcctg aaactagtat tacggaatta gctgatcgat taggcgttac
      301 caaaggagct gtatcccaac agattcccac tttagagaaa atgaatctta taagcatttc
      361 tcaaaaaaaa ggaaataaga aaactaaact tgtatcgtta acaaaatatg gtcgaaaggt
      421 gtacgaatca cataattatt tacatgaaga gctttatagc tcaataaatg aagcgttaga
      481 atcattctca ccagaccaaa tttcaaccat taaacagatt ctagaaaaag tatcttatac
      541 aatcaatgaa tatcaagtaa gtctgacaag aaaggaagat tgacatgaaa aaaatgattt
      601 ttgaatcggc tacactagac tagacagaaa aaataaggtg tgtaaaataa atgaaaacac
      661 actggaggaa tttttatgtc aagacgtcaa cgtagaacct attcaaaaga attcaaacaa
      721 caagtcgtcg atctttatct cgcaggaaag ccccgagctg aaatcattcg agattatgag
      781 ctgactacct cggcctttga taagtgggtc aagcaagcgc aaactactgg ctcgtttcag
      841 gaaaaagata acttgatccc tgaacaagta gaactaattg cattacgaaa gaaaaataaa
      901 cagcttgaaa tggagaatga cattttaaag caagcggcgc tgatattggg acgaaaagac
      961 aagtaattga taccaataag cacaaatact caatatcagc gatgtgtaaa gtgttaaaca
     1021 tctcttgcca aacctattat tatcaagcca aaccagttga agatgaatca gaactggaag
     1081 aactcgttca agaagaattt attcgcaatc gaaaagctta tggtaccagg aagctgaagc
     1141 agtgcttggc caatctaggt cttcaagtga gtcaacgcag aatcggccgt atgatgaaac
     1201 agcgtggctt gcgttcaaca tacacattgg ctcatttcaa attccattct tctacttgta
     1261 acgaagccaa gaccgccaat attctagacg gaaccttcct acaggaaaag tcattagaag
     1321 cgattgtaac cgaccttacc tatgttcgtg ttgggaagaa atggcagtat atctgcctga
     1381 ttcttgattt gtttaatcga gaaatcatcg gctattcatg cggggagaag aaagatgcga
     1441 cgttagtcaa ggaagctttt gcaaagattc cgtactcgtt gaataatgta caactttttc
     1501 acacggatcg tggaaaagaa ttcgataacc agacgattga cgaaatttta tgggccttta
     1561 atatcacgcg ttccttaagc aaaaaaggct gcccctatga taacgccgtt gcagaatcca
     1621 cctataaatc tgtcaaagta gaattcgttc atcaataccg ctttgagaca ttggctaatt
     1681 tgcgattaga gctatttgat tacgtccatt ggtggaatac catccggctg cacggcacgt
     1741 taggctatga gacaccgatc agttttcgac agcagagatt ggcgcagcga atccttgata
     1801 atgagctcgg atgtgatacc ggtggcgagg caccttaatt atttgaatga gcttctgcct
     1861 tagagaatca gattcgagga ggctcgttgt caagggcagt cggaacacca gcagcaagct
     1921 tacgcaatac cttataattt ttgtcaaaaa aagtgttgcc attccacata tatctttaaa
     1981 cagctattgc cagataaaaa taaatgatat tatatatata aatacttatc cgaaaagggg
     2041 aatgagagag tcaaggttct tggaatgtcg aatagtcgag taggtagatt ttctatctac
     2101 aaccttgttg tttaaaaatg aaaaaaattg gaattagaga agttgcgaaa gaggcaaatg
     2161 tttctcctac gactgtgtcg agggtattga atgatcgtgg atatttgagt gaggaaacaa
     2221 aaagaaaagt ttttgatgcc atgaaaaaat tagaatacta tcctaatgaa ctggcgagag
     2281 cactttttaa aaacgaaaca tataccgtag gtttgatttt tcctacaatt actaatccat
     2341 ttcacgctga gttaattcaa gatatcgagt tcgagctttc cagcagggga tataaagtgt
     2401 tgctgtgcaa tagcttaaac aatccaacaa aagaaaaaga gtacttgacg atgttgagaa
     2461 agaatcaggt tgatggaatt atagtaggaa cgcacaataa agatattgcg gattatgata
     2521 ttccagggct acctattgta gcgattgata gaagtcttgg tgaaaataca gttacagtat
     2581 cttgtgataa ttacaggggt ggggaattag ctactcaact acttgtaaat catggctgtc
     2641 agaatatact gtgtattcgg ggagatagta agataaagct acccgcgaat agtaggactt
     2701 tagcatataa agatgttttg aataaattca tgctggaacc gttagtccta gaagtaccat
     2761 ttgtaaaaga aatcaatgaa aaaaatgaaa taattaagac atatctggaa actcatccaa
     2821 atattgacgg ggtttttgca ggagatgatt tattggcttc tttggcgatt aacaacttag
     2881 gaagttcggg caaaagagtt ccaaaagagg ttaaagtgat tggatttgat ggagctcaac
     2941 aaacattgat ttataatcca gaattatcaa ctattcaaca gcctatagat caaatatcta
     3001 aagtagcagt aaaaaaatta ttgaatatga ttaagggcga aaaggagacg gatgaacttt
     3061 cacttccagt aaagctcata aaaaaaaata cagcataagt taaatcggcg aaaaattcct
     3121 tcgtcgattt aaaaatattt ttatgaaacg ggttgacata tgaaacgggt tgacatatat
     3181 tagttgtgaa gataaaaacc ttttcactgg aggaagaaaa tggagaaatt attaattaaa
     3241 agcaataatc atcaacttta ctaccaacct aaggatgttt gggtcggaga tgttatgcct
     3301 tacgggaaag aaggaaaatt ctttatttat catcaacgtg atactagaaa tccagttcct
     3361 tttggagaac cgtttggctg ggctttagca actacgaaag attttgttca ttttgaagac
     3421 tttggggaat ctcttttaag aggggatgac aatgagagag accagtttat ctacgctggt
     3481 tctgtatttg aggcaaatgg aaaggttcac gcattttata cagggtataa tcgtgaattc
     3541 ttagcgcagg gaaagacatc tcaagtgttg cttcatgctg agagtgatga ttttgttcat
     3601 tgggaaaaat cagaaacagc attggagtta gaaccacaag aagggtatga tattcgaaac
     3661 tggagagatc catttgtcgt ttgggatgat gaaaaagaag aatatttgct aattttaggg
     3721 gcgcgaaagg gtcaggataa acatctacaa accggacgtt tagtggaatt tacatcaaaa
     3781 gatttaacta attgggaatt caaaggtgat ttttgggctc cgggattgta taccatgttt
     3841 gaaatgccag aacttttcaa gattggtgac tggtggtatc ttgtctattc agaatactca
     3901 gatggaaata aaatactcta tcgaatgagt caaagtttgg aaggtccttg gattaagcct
     3961 aaagatgaag cttttgatgg acgggcttat tatgcaggta gaacagctta tgatggaaaa
     4021 cgtcgagtcc tatttggatg ggtaccaaca aaagatcaag aaaatgataa aaacaattat
     4081 ttatggggag gtactttggt tcctcatgaa gtctatcaaa aagaggattt ttcacttggg
     4141 gtaaaaccca tagacgaagt gtggaatgca ttcataaatt tacatgatat tgccgatgta
     4201 gagatagaaa cagcagacac aagagaagaa gtaacgcttg tcaaaaatag tggaactttg
     4261 tttaatttag aaacaacaat ttcatttgat gagaatctta gtgacttttc actgagatta
     4321 tataaaaacc ttgagacaga tgaatcttac gaatatcgct tcaatttaga tgaggggcgt
     4381 cttacatttg ataaaaaccc ttgttaccct tggtttcaat atatggataa aggacttatt
     4441 agacctatat ctttaaagcc taaccaatct tataaactaa aagtgattgt tgatgacaat
     4501 ataatgacac tttatattga tggaatcgct cttaacgctc gtgtacatgg ttgttttggt
     4561 caagcaatta ctattacagc aacaaatgga tcaatgactg caataaatac caagttttca
     4621 aatgaaataa agaaataata aacgtcaatc atgtggcagg tctgaaaatc aaacatgacc
     4681 tgtcacattt aaaagggagg ataaatgaat ggactacaaa aaaattgccg aagatgtatt
     4741 ggaaaacgtt ggaggaaaag agaatatctc aagcgctaca cactgcatta ctcgtttacg
     4801 tttaattctt aaagacaaaa gcaaagcgaa tatggaaatg cttgaggaaa tagaaggtgc
     4861 aaaaggtgtt cttttcaata gtggacagtt acaagtaatt ttcggaacag gtataattga
     4921 aaaggtttac aaagagttcg ttgaattgac aggagctaag gaagtatccg tttctgaaat
     4981 gaaagacgaa ggagtcaata attcaggaaa tagacttcaa cgagctttta aagttttctc
     5041 ggatatcttt attccgatca ttccagcttt tattggggca gcaatggttt taggaattcg
     5101 ttcgttgatt accactcctg gaatgtttgg aatggatggc tcattcgctg atcacaatgt
     5161 attcctaaat gatttatcca aatttctggg tattattgca accacatttg attatttacc
     5221 agttttagtt atgtattctg caacaaaacg ttttggcgga aaccctattt taggattact
     5281 tgtaggattt gttatgattc atccaggact agccaatcgg aatgatttcg tcatgggaac
     5341 tataaagcct gattattggc atttatttgg attaagcatt ccagcagttg cgtttcaagg
     5401 gggtgttttt ccagcaattt tgacttcttg gtttttagca aaagttgaga aaattacaaa
     5461 taaatattct cctcaagtac ttgcttacat tttagtccca actgtgacaa ttgttcttgc
     5521 caactttgca ctatttttag tgtttggtcc attggggaat tttatcggta atgttttagg
     5581 tggaatcatt gatttcctat atatgaagat gggtgccttt ggtgcttttg tttttgcagg
     5641 cgcgttacaa ccattagttg taacaggaac acaacatgct attcaaggta ttgaggcaaa
     5701 cttagtcgca acaactggat tcaattatat ccaacctatt tggtcagttt caattattgc
     5761 ccaaggtggc ggttgtattg gtatgtattt actctttaag aaaaaatcta aagatagaga
     5821 tattgcgatt tcaagcttta ttccaacact ggtaggtatt actgaaccgg ctatctttgc
     5881 tgttaatttg aaatattcaa tcgttccttt tgtttgctcg tttattggtg ctggttttgg
     5941 tggagcattt atgaaaattt ttgatgtaaa agccattgga caaggattga ctgtattgcc
     6001 aggtctgact attgctaatc cagttcttcc ttatatattt ggtaatctca tagcattcat
     6061 tttaccaatc atttttattc tcgcttatga taaatataaa gtgtttatcc ctgaagctaa
     6121 acaccgtaaa tttgcagaaa aaactgactc aaaaaaattg ccagttcaag taaagtgaga
     6181 tgagagcatg ttaaattttt ttagaaaaaa tgaaataagc gttgtagcac ctgcaaccgg
     6241 tgtttttgaa aaactggaga aattaagtga tccagtcttt tcaaaaggaa tgatgggaca
     6301 aggatttgca attgatgcaa aaaacgaaac gattgtatct ccaatcgatg gttttgtttc
     6361 aagtattttt cctacaaaac atgccttagg aataaaaaca aaatcaggaa ttgagatact
     6421 tgttcatgta ggaatagata cagttgagct aaatggtgag ggatttgata ttaaagttca
     6481 agaaggacaa aaagttaagg ctggggatat tcttatgacc attaattttg tagtttttga
     6541 aaagaatcac aagactaaag atgtcattgt tgtttttcca gactaccaag acgaaatttt
     6601 cattccgaaa cttgaagaag taaaacaagg acaagaattg tttgttcttg gctaatgata
     6661 agtaattaat aatagaatcg aggaaaatgt atggtttttg gggctattga agctggtgga
     6721 acaaaatttg tttgtgccat tggagataaa gatttgaaca tatttgaaaa agtcagcttt
     6781 cccacgacta cacccaaaga aacaatgaat ttagttttag atttttttaa tcatcataaa
     6841 gatgatttag aaggtatcgg agtgggatcg ttcggtccta ttgatattca ttgtgaatct
     6901 aaaacgtatg gcttcattac atcaacgcca aaacttgctt ggcaggattt tgattttatt
     6961 ggaagtttaa aaaagcaact taatattcca actgcttgga cgactgatgt aaatgcctct
     7021 tgctatggtg aatatgtttc tggaaatggc agaggccttt caagtgttgt ttactatacc
     7081 gtaggaacgg gcataggagg tggcgctatc caaaacggaa acttcattga aggttttagt
     7141 catccagaaa tgggacatat gcttgtaaaa cgacacaata aagacaactt ttctgggaca
     7201 tgtccattcc acggagactg tttagagggc ttagctgctg gtccggcaat cgaaagaaga
     7261 tcagggaaaa agggacaaga agtgactgaa aatgacgtta cttgggaaat agaagcttat
     7321 tacattgctc agtgtgcata taatacaaca ttgatgttat cacctgatgt cattatattt
     7381 ggtggcggag taatgaaaca aaaacactta aaaaagaaag tacaagataa gtttattgag
     7441 ttgatcaacg gatatgtcgc tattcccgat gttgacaatt atattatgac accaaaattg
     7501 ttagataatt ctggtattgt aggttgttta gctttagcaa gagacgttaa agaaaaatct
     7561 taatttggaa aaaagatcgt ctcgactatt tgtttggttg atgcgatctt tttttcaatt
     7621 agtaattgaa gggacgccta aaagtgaaaa aaaaaatgaa agatgaagta ttagagtata
     7681 tcaaaaataa tttaaagtat tacgattttt ctgctcaaga tattgcaatg aaattttgta
     7741 ttaaaagaaa tgtcgcttcc cactatttga atcagttatt ttcagatgga aaattactaa
     7801 agaatgattc tgttagacca gtaatgttca agtataatca acaaaagcca aaagactgct
     7861 tttctaagtt tattggagca gacatatctt tgaaatccac tatagataaa tgcaaagcta
     7921 ctgtaatgta tccccctaat ggcctcccat taataataaa aggaaacagt ggcgttggaa
     7981 aaagtttttt agcaagtttg atctatcagt atgctcttga tagaaaagta attcataatg
     8041 acgcaaaatt tgttgttgtg aattgtgcag attatgcaaa taatccagag ctattgtcag
     8101 cagttttatt tggatataaa aaaggagcct ttactggggc tgaaaaagat actgcaggga
     8161 tgctatcttc tgctaatgga ggataccttt ttttagatga ggttcacaat ctaagtgcag
     8221 aaaaccaaga gaaattgttt cttcttatgg attctcaaaa gtatcgtaaa cttggtgaaa
     8281 gtgtgcaatg ggagtatgca aatgttcgcc ttatcttagc cacaacagaa gataaaaata
     8341 cctctttgtt agcaactttt agaagacgca tcccagctga aatcactctg ccagattaca
     8401 ataatagaag tacatctgaa aaaattcaat tattatttga atttcttaaa gatgaagcaa
     8461 ttacgattga ttcaaaaata ttttgttcga ttgaactttt tactgatctt ttatcgaaaa
     8521 catttgaagg aaatgttgga gaattgagga atgaaatcaa gcttctttgt gcagaaggtt
     8581 atttaggaaa taagagaaag tcttcaattt atttagggga caaactagac agcggttttt
     8641 ggatagatcc agaaattagt atagatttaa aaaaaatgtt tcttaaatca ctttctgaaa
     8701 ttgatttgat tgaacttttt caaaatcaca tttcaattga agaaagcatg agtcaattaa
     8761 gagatagaat acttaaagag tgtgcaggtt cttctgagta taattattta gaaagtgacg
     8821 aatttatagc attaagaaac tatgttgtta ataaaatatc accaattatt gattcgacag
     8881 gtctatgttt attagatgaa ttcttggcgg atatcagtct atttataatg tttattgatg
     8941 taattgaaga aacatcaaga aattttttgc tgtctaagtt cagaaaattt cgtatgaaaa
     9001 atgataaaca aaaattattg gcaaatgaga tttggagtag tttagaagtc gaagaaagta
     9061 aacaagaact tttgttgaaa tggatacttt ttcttgtaaa gaagttctat gtgaaaatac
     9121 cagaaaccca ttgtctgata gttatgcacg gtaaaataac agcttctgcg atcgcaaggg
     9181 aaacaaataa gttgctgaat acatatgttt atgaggcttt cgatatgcca attgaaggtg
     9241 agactagtga cttaattaac ctaatcaaca atttttgtaa aagtatagat acgacgaatg
     9301 gactcattct gctagttgat atgggatcat tggaacaaat gtatgaaaaa attgaatcca
     9361 atgtaatagg tgatttggta attttaaaca atgtttctac agctttagcg attgaatgtg
     9421 ggattcaagt ttgccaaaaa aagccaatta gtcattttta ccagatggat tttgattcgt
     9481 ttcaagttaa agttcagtat tataaaggat tatcacaaaa gaaaaatgta atagtatctt
     9541 gtctctctgg tgaggggatt tctgaaaaag ttaaggatat tttaaaacgg tatctcgata
     9601 atcaagtgga gattttgatt tttgatttta acgcattaaa gaaaatagca aaggaaaaag
     9661 atactatgat ttttaagaat acaatttgtg ttctttcaac cactgaattt tacattcaag
     9721 gaatcgattg tttaaattta gaaaatctaa ttaatgggaa tcaaacctta gaaaaattaa
     9781 ataagtatat ggactcggat agctgtgagt tctgcttaaa tgaattagtt aaactcttta
     9841 ctattgaagg cgctagtact aagcttcgct ttttagattc caaaaaagta ttcaatgaga
     9901 tagaaaaagt actatatttg tatgaaaaat attacgatgt caaaatccca agttttctga
     9961 gaattaatct atttctacat ctttctggaa tgattgaacg aattatgatt ggtgatggaa
    10021 taacaaatga acatttgaaa gagggagcgc aactatttga tactttttta aatgtatcaa
    10081 aagatttttt ttcagaaatc aaagatattt actgtattga aatacccaaa ggagagtatg
    10141 agttaatcta tacaattttt gagcagacta ttttctaaat agcatactag cgtctaaaaa
    10201 aatgaaaggg tgtccaaagt atatttagac gctcatttaa ttaatattta ctgtattttc
    10261 ccgctttatt ggataatgtt agttggcaca gtaattgctt atataaaagt ataagaagaa
    10321 aggagagaac aaaatggcgt tttggcaaac atttttagtt gtgttgtatg gatttttcat
    10381 taactgggat aaggaagggc ccaaaatcgg aacctcccaa cctgtcgttg caggtttctt
    10441 gaccggactt attatgggag atattcaaac tggactttat atcggtggga ctttgcaatt
    10501 aatgacacta ggtattacta gttttggtgg tgcatcagta ccggattatc aaacagcagc
    10561 attgatcggt acttatttag caatctcaac taaccaggat gcttctatag gaataacttt
    10621 agcaataccg gtagcaatgt taattgtaca actagatgtt ctaaagtggt caacaaacat
    10681 tttttttcaa caaaaggcag aaaaattcgc acaaaaagaa gattatcaaa aattagagtg
    10741 gatgcaatat ttggcatcaa ttaatacaag ctttgtatct ggaattcctg ttttgttaac
    10801 tgtgatatta ggcccaacgt tagtgggaag tgcaatcaat tatattcctg aatgggttac
    10861 cggtggttta aaggtagcag ggggactatt acctgctgtt gggataggaa tgctacttaa
    10921 atatttacct acaaaagatt actttgccta tttgttagtt ggtttcgttt tggcagttta
    10981 tttccaaata ccaattttag ggatttcgtt acttggcgct gcaatcgcac taatcgtata
    11041 taaaaataga tcaaataata ctcaagtagc accagctgca acaggaggtt ttgacgaaga
    11101 tgaataagaa actgacaaaa atcgacaaac aagtaatttt ccggtggatt ctcaatggtg
    11161 caacttcatt aaattatgaa aaaatggaag ggttgggtta tgcgtattct atgcttcctt
    11221 ttatcaagga cacatataaa gatgatcctg aaggaatgaa acaatccgtt ttagctcact
    11281 tacaattttt taacactacg ccgtatacag cgccttacat tattggtatg aatgttggaa
    11341 ttgaaaaaga agaaaaaaat aagtctttgg aagcagtaag tgctctgaaa acaggtctaa
    11401 tgggtccttt agcgggaatt ggagatagtg tatttgtagt tattccatgg actatttttg
    11461 gagcaattgc agctaatatg gcattggaag gtagttttgt tggaattatt ttgtggatac
    11521 tggcatcaat tttattaagg attactgctt atcctttata ccttgctggg aaagaatcgg
    11581 gtgcgagcct actaactcaa attgaaacaa aattaaagtc tattactgat gcagtatcaa
    11641 ttttagggtt aatggtggtc ggtgcactca ttgccacagt tgtaaaagtg aatgttggct
    11701 ttgaatttaa acaaggcgac ttaacaatga ccggaaatga aatctttgat caagttatgc
    11761 ctggtttatt acctgcatgt gtagtagcaa ttgtgtatta tttgttagga cgaaaggtta
    11821 aaccagttta tgtaattcta ttaatcttag tagtttctat tgcgttaaga gcgttaaata
    11881 ttttgaagta ggtgttgaat atggatgaaa aaatgaaagg tattatacat gttcgtattg
    11941 atgatcgaat gattcatgga caagtagcaa cacaatggac aaatcaattg agtgctaaca
    12001 gaattatggt tattaataat gaagtagcca atgatgacgt aaaaaaagct gtagttcgcc
    12061 tcgctgcacc acctaatgtt agaacttcaa taattacaag ggaagtagca gttaaaaata
    12121 ttctttctgg taaatatgaa ggacaaaaag ttcttattgt tgcagtttct ccaatcgatg
    12181 ttaaattttt gattgataac ggtttaccaa taacaagtgt aaacgtagga aatttatcaa
    12241 gaaggaatgg aacagaacgc atccgtccga cgataaatat tactgaagaa gagagagagg
    12301 cttttaaaga actaattagt gatggagttg aagtgacagt gatacaaact cctagagatt
    12361 caaaagaatt tttaaaggat tttatttatt aagaacagag gatgagtgga tggagtttat
    12421 aattacatct catggaaata ttgccattgg atttgtagat acattaaaaa tgttttttgg
    12481 cgataattta aattgtcatg tggtaacact tggagatgca ggcattgaag aattccgtga
    12541 aaacattgaa aaaatagttt ctcaagtaaa aaatcaagaa gttatggtat ttgctgactt
    12601 gattggtgga acacctttca acgaatttgc taaaagaagt gaagtatttc aaaatgggtt
    12661 tcaacttctt ggtggcatga atttaggaat tttgattgag gctgtcaatt tgagattaca
    12721 aggaaaaaaa agcgatgata tcatcgaaaa tctaagaaat atgaatacaa ttgcgtgtct
    12781 tgcagatatg caaagacata ctaacgaaga ggatgagtga taaatgcaaa aaatttttta
    12841 caagcctaaa gatttttggt ttggggattg catgcctttt tataaagatg gaacttttta
    12901 tctatttcat caacgagata cgagaaatcc taaaccttta actgatcctt ttgggtggtc
    12961 actagttaca accactgatt ttgtgaaatt tgaagaatac ggggaagtaa ttcatcgtgg
    13021 tggcaacgat gacattgatc aatacattta cgctggaagt attgtccaag gaatcaatga
    13081 gtatttagct ttgtatactg gacataatcg caaagctaag ttggatggaa aaacttcaga
    13141 agtgttaatg attgcaagtt ctaaagattt aattcattgg aaaaaggatg gcgaagcagc
    13201 aaaacttgtt cctcaagaag gatatgataa gaatgattgg cgcgatcctt ttgcaatttt
    13261 tgatcaagat catggaaaat acgttttgat actaggagct agaaaaccag caagtaagaa
    13321 aaagccgacg gggagattgg tctattttga atccgaagat ttaaaagatt ggaatttcaa
    13381 aggagactac tgggctaata atgaatttaa catgattgaa atgcctgata tatttgaaat
    13441 tgatggaacg tggtatttac ttttttcaga gtattgtgaa gataaaaaga ctaaatatcg
    13501 agtcggagca ggattatttt cagattggaa atcaccagta gacgaagctt tcgacggaaa
    13561 agcctattat gcagcaagaa cagtcggaac taatgaaact ggccgttttc taactggctg
    13621 ggtggcaaca aaagaagggg agaacgatca aaataactgg gattggggcg gtgcacttgt
    13681 tccacatcaa attattcaaa agacagacaa aactcttggt gttaaactgc ctgaaaaaat
    13741 aattgagaat tttaaagaaa tcactccatt ttcaagtttt aagttagaga aagaatttgg
    13801 aaaaaaagaa agagtaattg ttcctaaaat cgaagattat tttctatttg aaacaacttt
    13861 gactattaca tcgttaacaa aggagtttgc tttaaaatta tttgtagata gtgatactca
    13921 agaaggatac gaatttcgta tttcattgat taataatcag cttactttcg gaaaaactcc
    13981 gaactatcct tggcctcaaa tgtttgataa ggggctgcaa cgaccactat ttttggaaat
    14041 aggcaaagaa tatgaactaa agttaattgt tgatgatacg atagctgttt tatatattga
    14101 taatgttgct ttaagtacta gaatgtatga taaagtcgga acagcattaa gtatgactgt
    14161 tacggaggga gcaatcaagg tagataaact agactattca ataaattatg aatgtggatt
    14221 ttaaaacaat tgaagggatg atgtaagtga aattaaatgg tttacaacat attggaattc
    14281 caacaaaaga ttttggagct tcaaaaatct tttatgaatc cttagggttt agtctgatta
    14341 atgaagaaat gaatgtggga agtaaagtcg gctttttcga gttgaatgga acgataatag
    14401 aaatatggga agataaaact actgagtgta gaggagccat tgatcatcta gcattagata
    14461 cggatgacat agatgaggta ttcaattcaa ttcaagattt aggatatgaa ttattagata
    14521 aaaatattat ggaattaccg ttctgggaaa atggaatccg ttactttaat ttctacggac
    14581 caaatcatga gattatagag ataagccaaa tgaagaaata aatagataac gaagtgctct
    14641 aagttggtat gaacgaaaag tacttcattt tttggtatca gcatttcaat agtttaatga
    14701 gaatttacta tttcaaatta attcaaagtg acagtatttt ttacttattt ggtttcaaat
    14761 tttgtaatcc ttcctattta ttggaataca ctccacattt tgtatacaag tcatatcatg
    14821 ttttagcaaa gctcttacca agcaaagtta taatgatgtt ttccaaagtt tgatctttcg
    14881 atggaggcta acacatattt tttgcaggac agccaaaatt agatgtatag aatagagtct
    14941 gtgaggcgta tagacagttt attgtctgta tgcctttttt tattttgaaa aatttttagt
    15001 atttttcgaa aaatgctcac tcctag
//
LOCUS       DAJSBM010000062        14939 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJSBM010000062 DAJSBM010000000
VERSION     DAJSBM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.062"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..562
                     /locus_tag="ONT67_002189"
     CDS             <1..562
                     /locus_tag="ONT67_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2355913.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="ONT67_002190"
     CDS             762..1076
                     /locus_tag="ONT67_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2355914.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="ONT67_002191"
     CDS             1097..1474
                     /locus_tag="ONT67_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2355915.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="ONT67_002192"
     CDS             1484..2257
                     /locus_tag="ONT67_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355916.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="ONT67_002193"
     CDS             2279..3682
                     /locus_tag="ONT67_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2355917.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="ONT67_002194"
     CDS             3864..5048
                     /locus_tag="ONT67_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2355918.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="ONT67_002195"
     CDS             5045..5335
                     /locus_tag="ONT67_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355919.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="ONT67_002196"
     CDS             5332..5553
                     /locus_tag="ONT67_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355920.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="ONT67_002197"
     CDS             5595..6374
                     /locus_tag="ONT67_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCR2355921.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="ONT67_002198"
     CDS             6441..6935
                     /locus_tag="ONT67_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2355922.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="ONT67_002199"
     CDS             6991..7629
                     /locus_tag="ONT67_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355923.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="ONT67_002200"
     CDS             7701..8096
                     /locus_tag="ONT67_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2355924.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="ONT67_002201"
     CDS             8080..10533
                     /locus_tag="ONT67_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2355925.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="ONT67_002202"
     CDS             10530..12557
                     /locus_tag="ONT67_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355926.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="ONT67_002203"
     CDS             12554..13576
                     /locus_tag="ONT67_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2355927.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="ONT67_002204"
     CDS             13593..14522
                     /locus_tag="ONT67_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2355928.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="ONT67_002205"
     CDS             14767..14883
                     /locus_tag="ONT67_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2355929.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         5072 a         2485 c         2954 g         4428 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgc
//
LOCUS       DAJSBM010000063        14482 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJSBM010000063 DAJSBM010000000
VERSION     DAJSBM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.063"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(287..649)
                     /locus_tag="ONT67_002206"
     CDS             complement(287..649)
                     /locus_tag="ONT67_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355930.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ONT67_002207"
     CDS             complement(723..1103)
                     /locus_tag="ONT67_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCR2355931.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="ONT67_002208"
     CDS             1167..2360
                     /locus_tag="ONT67_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2355932.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="ONT67_002209"
     CDS             complement(2437..3021)
                     /locus_tag="ONT67_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2355933.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ONT67_002210"
     CDS             3222..4220
                     /locus_tag="ONT67_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2355934.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ONT67_002211"
     CDS             4332..4841
                     /locus_tag="ONT67_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR2355935.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="ONT67_002212"
     CDS             complement(4871..5530)
                     /locus_tag="ONT67_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCR2355936.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="ONT67_002213"
     CDS             complement(5532..6296)
                     /locus_tag="ONT67_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCR2355937.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="ONT67_002214"
     CDS             complement(6304..6981)
                     /locus_tag="ONT67_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCR2355938.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="ONT67_002215"
     CDS             complement(7018..8403)
                     /locus_tag="ONT67_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR2355939.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="ONT67_002216"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ONT67_002216"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT67_002217"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="ONT67_002217"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR2355940.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="ONT67_002218"
     CDS             9342..10199
                     /locus_tag="ONT67_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355941.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="ONT67_002219"
     CDS             complement(10280..10615)
                     /locus_tag="ONT67_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355942.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="ONT67_002220"
     CDS             complement(10682..12052)
                     /locus_tag="ONT67_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCR2355943.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="ONT67_002221"
     CDS             complement(12130..12666)
                     /locus_tag="ONT67_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2355944.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="ONT67_002222"
     CDS             12975..14456
                     /locus_tag="ONT67_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2355945.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DAJSBM010000064        14107 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJSBM010000064 DAJSBM010000000
VERSION     DAJSBM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.064"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            66..767
                     /locus_tag="ONT67_002223"
     CDS             66..767
                     /locus_tag="ONT67_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCR2355946.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="ONT67_002224"
     CDS             764..1885
                     /locus_tag="ONT67_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR2355947.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="ONT67_002225"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="ONT67_002225"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR2355948.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="ONT67_002226"
     CDS             3264..4172
                     /locus_tag="ONT67_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCR2355949.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="ONT67_002227"
     CDS             4207..4479
                     /locus_tag="ONT67_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCR2355950.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="ONT67_002228"
     CDS             4490..5536
                     /locus_tag="ONT67_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCR2355951.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="ONT67_002229"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="ONT67_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR2355952.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9233)
                     /locus_tag="ONT67_002230"
     CDS             complement(8547..9233)
                     /locus_tag="ONT67_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355953.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9211..9705)
                     /locus_tag="ONT67_002231"
     CDS             complement(9211..9705)
                     /locus_tag="ONT67_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCR2355954.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="ONT67_002232"
     CDS             complement(9725..10945)
                     /locus_tag="ONT67_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2355955.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="ONT67_002233"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="ONT67_002233"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR2355956.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..>14107)
                     /locus_tag="ONT67_002234"
     CDS             complement(13021..>14107)
                     /locus_tag="ONT67_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2355957.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DAJSBM010000065        13852 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJSBM010000065 DAJSBM010000000
VERSION     DAJSBM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.065"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..902)
                     /locus_tag="ONT67_002235"
     CDS             complement(<1..902)
                     /locus_tag="ONT67_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCR2355958.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ONT67_002236"
     CDS             1185..2153
                     /locus_tag="ONT67_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2355959.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ONT67_002237"
     CDS             2266..2544
                     /locus_tag="ONT67_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR2355960.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ONT67_002238"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ONT67_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR2355961.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ONT67_002239"
     CDS             3001..3756
                     /locus_tag="ONT67_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2355962.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ONT67_002240"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ONT67_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR2355963.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ONT67_002241"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ONT67_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR2355964.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ONT67_002242"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ONT67_002242"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR2355965.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ONT67_002243"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ONT67_002243"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCR2355966.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ONT67_002244"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ONT67_002244"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR2355967.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ONT67_002245"
     CDS             8493..9035
                     /locus_tag="ONT67_002245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR2355968.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="ONT67_002246"
     CDS             9058..10614
                     /locus_tag="ONT67_002246"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR2355969.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ONT67_002247"
     CDS             10628..11530
                     /locus_tag="ONT67_002247"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR2355970.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ONT67_002248"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ONT67_002248"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR2355971.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ONT67_002249"
     CDS             12973..13395
                     /locus_tag="ONT67_002249"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR2355972.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4575 a         2474 c         3013 g         3790 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttttgtgg ctctttgtca ataaggactg atgagttgtg
    13741 caaaatcaaa tctgagtcag aatgaaccac attctggctc agattttttg ttatcttaca
    13801 ttgattaatt gattgatcaa aaagattcta attctcatgt tctcaaatga tt
//
LOCUS       DAJSBM010000066        13841 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJSBM010000066 DAJSBM010000000
VERSION     DAJSBM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.066"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(143..1345)
                     /locus_tag="ONT67_002250"
     CDS             complement(143..1345)
                     /locus_tag="ONT67_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2355973.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="ONT67_002251"
     CDS             complement(1348..1542)
                     /locus_tag="ONT67_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCR2355974.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="ONT67_002252"
     CDS             complement(1589..2437)
                     /locus_tag="ONT67_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HCR2355975.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="ONT67_002253"
     CDS             complement(2642..2992)
                     /locus_tag="ONT67_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355976.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="ONT67_002254"
     CDS             3587..4033
                     /locus_tag="ONT67_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCR2355977.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="ONT67_002255"
     CDS             4657..5649
                     /locus_tag="ONT67_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR2355978.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="ONT67_002256"
     CDS             5665..6549
                     /locus_tag="ONT67_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR2355979.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="ONT67_002257"
     CDS             6598..8205
                     /locus_tag="ONT67_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2355980.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="ONT67_002258"
     CDS             8206..9690
                     /locus_tag="ONT67_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR2355981.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="ONT67_002259"
     CDS             9867..10859
                     /locus_tag="ONT67_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2355982.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="ONT67_002260"
     CDS             10911..11780
                     /locus_tag="ONT67_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2355983.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT67_002261"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="ONT67_002261"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR2355984.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4241 a         2343 c         2856 g         4401 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catatttttt tagttttcaa agatcagacc t
//
LOCUS       DAJSBM010000067        13834 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJSBM010000067 DAJSBM010000000
VERSION     DAJSBM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.067"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            9..2174
                     /gene="acm"
                     /locus_tag="ONT67_002262"
     CDS             9..2174
                     /gene="acm"
                     /locus_tag="ONT67_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCR2355985.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2446..2931)
                     /locus_tag="ONT67_002263"
     CDS             complement(2446..2931)
                     /locus_tag="ONT67_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCR2355986.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3021..3366
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3411..4052
                     /locus_tag="ONT67_002264"
     CDS             3411..4052
                     /locus_tag="ONT67_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR2355987.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4071..4706
                     /locus_tag="ONT67_002265"
     CDS             4071..4706
                     /locus_tag="ONT67_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR2355988.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4724..5539
                     /locus_tag="ONT67_002266"
     CDS             4724..5539
                     /locus_tag="ONT67_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR2355989.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5858..7294
                     /locus_tag="ONT67_002267"
     CDS             5858..7294
                     /locus_tag="ONT67_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2355990.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7483..9930
                     /locus_tag="ONT67_002268"
     CDS             7483..9930
                     /locus_tag="ONT67_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2355991.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10160..11227
                     /gene="rlmN"
                     /locus_tag="ONT67_002269"
     CDS             10160..11227
                     /gene="rlmN"
                     /locus_tag="ONT67_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2355992.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11420..12523)
                     /locus_tag="ONT67_002270"
     CDS             complement(11420..12523)
                     /locus_tag="ONT67_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2355993.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13076..13624
                     /gene="aac(6')"
                     /locus_tag="ONT67_002271"
     CDS             13076..13624
                     /gene="aac(6')"
                     /locus_tag="ONT67_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2355994.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4735 a         2306 c         2927 g         3866 t
ORIGIN      
        1 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
       61 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
      121 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
      181 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
      241 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
      301 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
      361 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
      421 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
      481 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
      541 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
      601 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
      661 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
      721 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
      781 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
      841 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
      901 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
      961 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
     1021 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
     1081 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
     1141 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
     1201 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
     1261 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
     1321 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
     1381 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
     1441 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
     1501 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
     1561 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
     1621 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
     1681 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
     1741 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
     1801 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
     1861 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
     1921 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
     1981 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
     2041 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
     2101 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
     2161 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
     2221 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
     2281 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
     2341 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
     2401 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
     2461 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
     2521 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
     2581 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
     2641 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
     2701 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
     2761 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
     2821 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
     2881 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
     2941 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
     3001 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
     3061 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
     3121 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
     3181 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
     3241 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
     3301 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
     3361 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
     3421 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
     3481 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
     3541 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
     3601 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
     3661 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
     3721 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
     3781 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
     3841 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
     3901 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
     3961 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
     4021 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
     4081 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
     4141 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
     4201 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
     4261 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
     4321 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
     4381 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
     4441 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
     4501 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
     4561 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
     4621 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
     4681 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
     4741 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
     4801 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
     4861 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
     4921 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
     4981 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
     5041 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
     5101 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
     5161 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
     5221 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
     5281 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
     5341 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
     5401 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
     5461 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
     5521 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
     5581 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
     5641 acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc aaatttatgc
     5701 aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta agcgactttt
     5761 tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt tattctaaaa
     5821 gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag aacttacctc
     5881 atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag aaggagcttg
     5941 gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac ctggaaagac
     6001 atttaaagga acgaacggtg atttggcagt agaccactat catcgttata aagaagatat
     6061 tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt ggacacggat
     6121 ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg agagattgat
     6181 cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt gggatcttcc
     6241 acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg aagatttcac
     6301 tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact ggattagttt
     6361 gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc cgccaggtgt
     6421 gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa atgcatctgt
     6481 catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct ttgcgtatac
     6541 gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa atgcagaaga
     6601 cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga ttgctgccat
     6661 gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg cagaattgct
     6721 agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga cgaatgcatt
     6781 caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa aaggttcatc
     6841 agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg tcgaacgaac
     6901 aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc gtatcacaag
     6961 ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg acaagttgac
     7021 agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc acgtcaaagc
     7081 catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct ggtcttatac
     7141 agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat atgttgatca
     7201 agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt actggtacca
     7261 aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac aagaaggtac
     7321 ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga aaaactgtaa
     7381 aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta gttcccttac
     7441 ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca aaagaaacgg
     7501 cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa
     7561 atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg ctttttaaaa
     7621 ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg aaatacttac
     7681 caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa gaatacagag
     7741 ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt cggtatctta
     7801 ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa tatcatgggg
     7861 acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa taacgttggt
     7921 ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc acagcctgga
     7981 agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag taatctggaa
     8041 ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga attattggaa
     8101 ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc
     8161 gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca
     8221 gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga tgaatggcag
     8281 gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag ttttcaagaa
     8341 aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca gaccaataca
     8401 gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga atttgagatt
     8461 gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct tgattctata
     8521 ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa
     8581 caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc
     8641 gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat tgtgaactta
     8701 acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc accgattcca
     8761 ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt ttcctttaga
     8821 gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc tttaggacgt
     8881 gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt actcgtggct
     8941 ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag tatattaatg
     9001 cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt tgaattgaat
     9061 atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg
     9121 caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc
     9181 ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga
     9241 acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc tgatttgatg
     9301 atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat ggctcgggca
     9361 gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat cacaggaatc
     9421 atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac agattctcgg
     9481 acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat gctattcttg
     9541 ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga ccacgaagta
     9601 gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga gaaaatgatg
     9661 ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga gctgtttgaa
     9721 gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct ccaaagaaga
     9781 tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc tcatggagtt
     9841 gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga agaaacatca
     9901 gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa
     9961 taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt tttccatctt
    10021 ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt tctattcgat
    10081 ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc gaaactttga
    10141 gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga atgaagaatt
    10201 aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag tttgggaatg
    10261 gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga aacaattgat
    10321 cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc
    10381 ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga ttgagactgt
    10441 actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat
    10501 cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga ctgctggaga
    10561 aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc ttggcgaacg
    10621 cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg acaatgtaat
    10681 ggactttttg catatcatca atgatgccaa aggattggca attggtgctc ggcacattac
    10741 agtttccact agtggattag cacacaaaat caaggaattc gctaataacg gcttgcaagt
    10801 caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga tcatgcggat
    10861 caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt tagagaaaac
    10921 aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata gaccagagca
    10981 tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg tcaacttgat
    11041 tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag ctgatgtatt
    11101 gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg
    11161 tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc
    11221 caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt ttcttaatta
    11281 aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata aagctttttt
    11341 tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga
    11401 tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt tcttgtgtag
    11461 catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg atttcataag
    11521 gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct aaatcggtcg
    11581 tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc ataaacacat
    11641 agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt acaccataac
    11701 tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta ccgtaataga
    11761 atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc aatccggtag
    11821 taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg tcggcactat
    11881 ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt ccgatttgga
    11941 ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta gattcgactt
    12001 ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct tcggcaataa
    12061 ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt ccgtccatta
    12121 cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc gaaccaatag
    12181 tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca gtatcgcaag
    12241 cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca tgctggatca
    12301 actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg acaacctcta
    12361 tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca gtcttccctc
    12421 caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt tcatacgcat
    12481 ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt tccttctttc
    12541 ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct gtagatggag
    12601 tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac cttgaaatca
    12661 tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa taatgaattt
    12721 ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc taaaaattct
    12781 aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat tggttttttt
    12841 taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc caattgatgg
    12901 ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt attttataag
    12961 taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt tgagcgagaa
    13021 catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa gacgtatgat
    13081 aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg atttactgag
    13141 actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag aaatgatgaa
    13201 tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat ttattggtgc
    13261 aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag aaagctcccg
    13321 acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag tagcttccag
    13381 aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa cgttaagtca
    13441 aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc ttcgtgaaca
    13501 tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac caaatgcaaa
    13561 tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac caaattctca
    13621 ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag gaatttacga
    13681 aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct acttttggaa
    13741 caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga aaaattttgt
    13801 gaaaatggct ttctacattt ttgcataaat ttgg
//
LOCUS       DAJSBM010000068        13731 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJSBM010000068 DAJSBM010000000
VERSION     DAJSBM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.068"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..1452
                     /locus_tag="ONT67_002272"
     CDS             1..1452
                     /locus_tag="ONT67_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR2355995.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="ONT67_002273"
     CDS             1801..2433
                     /locus_tag="ONT67_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCR2355996.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="ONT67_002274"
     CDS             3021..4460
                     /locus_tag="ONT67_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2355997.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="ONT67_002275"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="ONT67_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="ONT67_002276"
     CDS             5222..5437
                     /locus_tag="ONT67_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2355998.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="ONT67_002277"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="ONT67_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6097..7008)
                     /locus_tag="ONT67_002278"
     CDS             complement(6097..7008)
                     /locus_tag="ONT67_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2355999.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="ONT67_002279"
     CDS             complement(7027..8031)
                     /locus_tag="ONT67_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCR2356000.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="ONT67_002280"
     CDS             complement(8028..10151)
                     /locus_tag="ONT67_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2356001.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="ONT67_002281"
     CDS             complement(10156..12603)
                     /locus_tag="ONT67_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356002.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="ONT67_002282"
     CDS             complement(12590..12979)
                     /locus_tag="ONT67_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2356003.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="ONT67_002283"
     CDS             complement(13039..13542)
                     /locus_tag="ONT67_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2356004.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..13716)
                     /locus_tag="ONT67_002284"
     CDS             complement(13555..13716)
                     /locus_tag="ONT67_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356005.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DAJSBM010000069        13398 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJSBM010000069 DAJSBM010000000
VERSION     DAJSBM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.069"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(14..706)
                     /locus_tag="ONT67_002285"
     CDS             complement(14..706)
                     /locus_tag="ONT67_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2356006.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="ONT67_002286"
     CDS             complement(1143..1712)
                     /locus_tag="ONT67_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2356007.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="ONT67_002287"
     CDS             complement(1786..2109)
                     /locus_tag="ONT67_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356008.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="ONT67_002288"
     CDS             complement(2263..3075)
                     /locus_tag="ONT67_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356009.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="ONT67_002289"
     CDS             3429..4277
                     /locus_tag="ONT67_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR2356010.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="ONT67_002290"
     CDS             4422..5363
                     /locus_tag="ONT67_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCR2356011.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="ONT67_002291"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="ONT67_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT67_002292"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="ONT67_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCR2356012.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="ONT67_002293"
     CDS             complement(9827..12013)
                     /locus_tag="ONT67_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCR2356013.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT67_002294"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="ONT67_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCR2356014.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="ONT67_002295"
     CDS             complement(12235..12675)
                     /locus_tag="ONT67_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCR2356015.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="ONT67_002296"
     CDS             complement(12749..13288)
                     /locus_tag="ONT67_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2356016.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DAJSBM010000070        13269 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJSBM010000070 DAJSBM010000000
VERSION     DAJSBM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.070"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="ONT67_002297"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="ONT67_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2356017.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="ONT67_002298"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="ONT67_002298"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR2356018.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="ONT67_002299"
     CDS             3349..3552
                     /locus_tag="ONT67_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR2356019.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="ONT67_002300"
     CDS             complement(3653..4315)
                     /locus_tag="ONT67_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2356020.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="ONT67_002301"
     CDS             complement(4328..5830)
                     /locus_tag="ONT67_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2356021.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="ONT67_002302"
     CDS             6378..8057
                     /locus_tag="ONT67_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2356022.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="ONT67_002303"
     CDS             8184..8663
                     /locus_tag="ONT67_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCR2356023.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="ONT67_002304"
     CDS             8882..9562
                     /locus_tag="ONT67_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR2356024.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="ONT67_002305"
     CDS             9570..10901
                     /locus_tag="ONT67_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCR2356025.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="ONT67_002306"
     CDS             10916..11878
                     /locus_tag="ONT67_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCR2356026.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="ONT67_002307"
     CDS             11989..12327
                     /locus_tag="ONT67_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCR2356027.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="ONT67_002308"
     CDS             12406..13113
                     /locus_tag="ONT67_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCR2356028.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4230 a         2338 c         2907 g         3794 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagggctc
    13201 tttgtcaata aggactgatg ggttgtacaa aatcaaatct gggtcagaat gaacctcatt
    13261 ctggctcag
//
LOCUS       DAJSBM010000071        12734 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJSBM010000071 DAJSBM010000000
VERSION     DAJSBM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.072"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT67_002309"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ONT67_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR2356029.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ONT67_002310"
     CDS             complement(322..891)
                     /locus_tag="ONT67_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCR2356030.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ONT67_002311"
     CDS             1077..1979
                     /locus_tag="ONT67_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2356031.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT67_002312"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ONT67_002312"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR2356032.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT67_002313"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ONT67_002313"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR2356033.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT67_002314"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ONT67_002314"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR2356034.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ONT67_002315"
     CDS             8547..8732
                     /locus_tag="ONT67_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCR2356035.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ONT67_002316"
     CDS             complement(8794..9990)
                     /locus_tag="ONT67_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCR2356036.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ONT67_002317"
     CDS             complement(10160..11596)
                     /locus_tag="ONT67_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCR2356037.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ONT67_002318"
     CDS             11889..12698
                     /locus_tag="ONT67_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2356038.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2675 c         2249 g         4007 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaacgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DAJSBM010000072        11990 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJSBM010000072 DAJSBM010000000
VERSION     DAJSBM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.073"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            81..1091
                     /locus_tag="ONT67_002319"
     CDS             81..1091
                     /locus_tag="ONT67_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR2356039.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1160..2104)
                     /locus_tag="ONT67_002320"
     CDS             complement(1160..2104)
                     /locus_tag="ONT67_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR2356040.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2088..4808)
                     /locus_tag="ONT67_002321"
     CDS             complement(2088..4808)
                     /locus_tag="ONT67_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356041.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4805..6025)
                     /locus_tag="ONT67_002322"
     CDS             complement(4805..6025)
                     /locus_tag="ONT67_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCR2356042.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6306..7151)
                     /locus_tag="ONT67_002323"
     CDS             complement(6306..7151)
                     /locus_tag="ONT67_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2356043.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            complement(7409..7648)
                     /locus_tag="ONT67_002324"
     CDS             complement(7409..7648)
                     /locus_tag="ONT67_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356044.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(7653..8072)
                     /locus_tag="ONT67_002325"
     CDS             complement(7653..8072)
                     /locus_tag="ONT67_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR2356045.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            complement(8299..8628)
                     /locus_tag="ONT67_002326"
     CDS             complement(8299..8628)
                     /locus_tag="ONT67_002326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356046.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            complement(8607..8930)
                     /locus_tag="ONT67_002327"
     CDS             complement(8607..8930)
                     /locus_tag="ONT67_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR2356047.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            9435..9788
                     /locus_tag="ONT67_002328"
     CDS             9435..9788
                     /locus_tag="ONT67_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356048.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(9848..10015)
                     /locus_tag="ONT67_002329"
     CDS             complement(9848..10015)
                     /locus_tag="ONT67_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356049.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(10134..11966)
                     /gene="tet(M)"
                     /locus_tag="ONT67_002330"
     CDS             complement(10134..11966)
                     /gene="tet(M)"
                     /locus_tag="ONT67_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2356050.1"
                     /translation="MTELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNII
                     DTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKID
                     QNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKY
                     TSGKLLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQS
                     ELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKI
                     DKAYSGEIVILQNEFLKLDSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLD
                     ALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIEIKEPTVIYM
                     ERPLKKAEYTIHIEVPPNPFWASIGLSVAQLPLGSGVQYESSVSLGYLNQSFQNAVME
                     GIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLE
                     PYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTF
                     FTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
BASE COUNT         3643 a         2316 c         1871 g         4160 t
ORIGIN      
        1 cgacaacgtt tttaaaggaa atgggatatg gtatagttaa tatataaaaa taataactaa
       61 ttacaggagt gcaaatttta atgaaaaaaa aatcaattat tttggcagct actagtgcgt
      121 tggctgtttt aactctagca gcttgttcag gcgatactaa taaagatatc gcaacgatga
      181 aaggcggaac gatcactgta tctgatttct atgatgaagc aaaacttgaa tcatcgaacc
      241 aatccttagt acaacggatg atcatttata aagtattcaa taacaaatat ggcgataaag
      301 taaccgataa gcaagtggat gcagaatacg acaaacaagc gaaatcatta ggtgatactt
      361 ttgaatcaca attagaagct gctggatata cgaaagatac ttataaagaa tatattcgta
      421 acaatttagc ttttgaagct ggcttgaaag ctcacgtaga tattacagat gacgatttga
      481 aaactgcttg gaaatctttc catccagaag tagaagcaca aatcatcaaa ttatcaagtg
      541 aagatgaagc aaaagatgtg aaaaaatcag cggatgatgg agatgatttc tctaaattag
      601 ccaaagacaa atcaactgat acagagacaa aagaagatgg cggtaaagtt aaattcgact
      661 caacgacaac aactattcct gcagaagtaa aagaagcagc tttcaaattg aaagacggtg
      721 agatctccga tgttattact acaacaaatc ctacttctta tgcgacagaa tactacgttg
      781 tcaaaatggt gaaaaaccaa aataaaggca atgacatgga taaatacaaa gatcaactaa
      841 aagatatcgc tactgaaact aaactaagtg acaatgcatt cacaactaaa gtaatcggtg
      901 aagaattaaa agatgcaaat gtaaaaatca aagatgatgc ctttgaaaat gtgttgagtg
      961 cattcacaac aacttcatca agtacgaaag actcaagtga aactacagct tcaacaaaga
     1021 gttctgacac aaaatcaact gattcaacaa aagaatcttc aactgaagaa acaactgatt
     1081 catcaaaata aaagaaaaca aaaaaagcga agacttatgt ctcgcttttt ttgttttgaa
     1141 tgaaaaaaat aaatttttgc tataaatctt ttggtacata gaaacttcgg ttgtccatac
     1201 cgaaaatccg atcggtgtat tttcctggtt ctgtttgacg aatcgaagtc aacatcatct
     1261 gcatcgaagc atcgatattg tcgatttgat gtaatatttc tgcttccatt attcttggac
     1321 gtacaggtga gccatattct aataagccat ggtgtgctaa aaccatatga cgtaatacaa
     1381 gaacatcttc gtcagcttcg ctgattttca acgcaagaca ggctttagtt atttcttcgt
     1441 caatcaatac cagatgaccg atcaaatttc ctgcaagcgt atattctgtc gacataggac
     1501 cggaaagttc aatcaccttt cctaaatcat gcaaaatgat accagcatac aacaatgctc
     1561 gattcagctc aggatattca tcggcgattg atttccctaa gtgaagcatt gtcaaagtat
     1621 gataagctaa tccactggca aatgcatgat ggtttctttt tgctgccgga aactcaaaaa
     1681 attgtttttg atatttatta agtagatagc gaacaatacg attccaatgc gcattggtga
     1741 tctcaaacaa caattgattg atttcttctt ccatttcttc tttcttcaaa ggggctcttt
     1801 ccatgtataa actaggctct cctggctcat ctacccttgt taagcgcata tgaagaatct
     1861 ttacttgagg gtttccttga taaacctctc ttttaccgtt tagaaaaacg actgtgccgg
     1921 ctgtaaattt attgatttcc tcctccgaag catcccagta tttaccatcg atagtacctg
     1981 aagtatcttg gaaagtaaaa gcaataaatt tttttccgtt tttagcgatt cttacatctg
     2041 cacttttgat caggacaaat tgttcaaact gctcatcgat tactaaatca cgaatttttt
     2101 tcattatttg ctccttctaa ttgttttacg atttggttca tctcatggta atagcttacc
     2161 atttctttat cagaagaaaa acaaattatt tgatactttt ctccaaattt agctaaaagt
     2221 tcagctaatt gatactttcg ctgatgatca taatgaagcc agccatcatc aatgatgatc
     2281 gggcatatcg aacggttagc ttctaatgag aggtaagcaa atctcatcgc cataatcagc
     2341 tgatctttcg tacctgtaga taactcatag atgggaaatt ctgtatatcc gtctgttaaa
     2401 gtcagtgtat catttttcag taatatcttc tgataatttc cttttgttaa aatacctaaa
     2461 taatcacctg ctgctcttaa caactggggt aattgtcttt cactcatctc tgttgaaaga
     2521 tcacctaaga atgtcccggc gatttcaagt gctccccatt cctccagtaa ttcctgtaat
     2581 tttgcttttt gccggctagc ttgttgataa agttcgtcca atgtgccgtc tctttgcaac
     2641 tcttctactt gaagttggag tcgttgccga ttttcacttg cttctttttc ttcttgctgg
     2701 agttgggatt gcttttcttt cagttggcgc agtcttacag ataatttctc ttttgtcagc
     2761 tcttcaggaa atagtggagt aagcatttcc gataattcat tgtatctttt catctgctgt
     2821 tcggcttgtt tggctttttg taaaaagaca ggtacttccg aaggataaga taagcctgct
     2881 tgagtcaatt gttcgctgta ttcttgtatc catacttttt gtttcttttt caattgattg
     2941 atttcttgct tcaataaagt attttgctga tagcttctgg caaatttcat ctgttccatc
     3001 tcttgtataa aacgggaaag caataccatt ttttctgaaa ttgtcttatc ttgtatcggt
     3061 aaccattctt taacaaactc aaatttttgg tcgatttgtc gtagtttttc ttgtatttcc
     3121 tgttctcttt tatgtttccg ctcaaatacg ccatataatt tctgataatc tatgacttcc
     3181 tgtccatatt ctttcatgag tttgagcgta tccatctttc ctaagtgccg atgatccgca
     3241 aaacgatata cttcttgctg aagaatctgt ttttcttctc cagttctctt ttgtttttct
     3301 tctgcttcag ctaattgagc taaggctaga tcaagttgtc ctagtttttc ttgccacatt
     3361 ccttttatct cagagaaatc atttttagtt ttaatttttg gttgcctttt gtagaagaaa
     3421 tagccaatca tgcctgcacc aattagtaaa aggatcactt tcaagggcat tggtacaaat
     3481 atacctgcaa tgattattgc tccgccagcc atcagcatat acggtttaga tgaagagact
     3541 ggtgctatct ctgactgatt ttgtaataat gctggatagg tctcttccaa gtcattcact
     3601 tctgcttcaa tcatctcttg ctgttcttta gctatttgga gacgcacaga aatctggctt
     3661 tctagatcag caatccttct ttcttcagcc agaagatgct tccattcttc ttcatataaa
     3721 aattcttccg gtaacggatt tttctgccaa tgccaccttt gttccaaaat cttcaattct
     3781 gaatttgttt ctctagtctc aatttgtaat ctttgccatt cttcgtttaa ttgttccgct
     3841 gtaaattgaa gttgctggag atcttttagt tcctgctcct gctctaaata aaaataatat
     3901 ctggctgact gctgatccat cccgctatgc ttttccagtg ttgcttctaa ttcttccaaa
     3961 cgctcatttg tttgctgata ccgtttggag aaagactcca gatctaaaag aagttgctca
     4021 tcaggaagac ctgtagtgct gttttttttg agagattgcc attcttcata taaagaaaaa
     4081 ttaagttctt gctgtctggc taatgaaaat tgtttgttcg cctcttttaa ttgtgccaat
     4141 aactgctgct gttgcgactc gtattcctgt tcttgttgga tcaacttgcc aaaataagct
     4201 tcctgttctt gtttttgatg aattttttct tgcagctctt tccattcatt taatttttga
     4261 ttgattagta atttttggcc ttttttcttg aataattgtt gtgctttctg ataatattct
     4321 tgccgctttt ggaatacttg agaactgccc gtgatgccta aagaaatcag cgcatcatgt
     4381 agctcttttt ctctcaaatg atctagttcc atcaactgct cttgctggaa agtaaatacc
     4441 tgctggaata actctttcgt caaaggtgcg atcagttttt ctagtaactc atcacttccc
     4501 acttgatccc ccagccacac cttcgatttt ccgcgatttt gctgcttgta gcgctcgaca
     4561 gcaaattcac catatacagg atgtttaagc catattttgc cgccataggc tgagccgtct
     4621 tttggtgtat aatccttttt tttacggcct tttgaaggga aaccaaataa tattgcttga
     4681 ataaattgat aaatcgttga ttttcctgct tcgtttcccc catagatcag ctgattgcct
     4741 gaataaaagt caaaactttt ttgacgccat tttccaaaag cagcaatctc gatccttaaa
     4801 atcttcattc ctgatcctcc tcaaataaaa aatcttgatt aagcagctgc ttggcttttt
     4861 caagtgtttc ttcttgatat tccggcagtt cgctcaatat tctagccgct tcttgatgac
     4921 tatatacttc atttaaaatt tgttggaaat cttgaggtgt ctgatagtat tgaaataact
     4981 gttccatcaa tttagcagaa gcagcaagtg gggtcttcgt ataaaaagta ttctctatga
     5041 gagaaatacg ccaaataaag aaatcattta tcctatctga gaattcctct aacaaatagt
     5101 ctaatagctc tcctgattgt atccgttcca aaacatctgt attgagatgg tcataatctt
     5161 ttaattttat ctcaattaaa ctaaatccat cctctacttt tgaaagctgc tgacgaatgt
     5221 gaacaatgac ctcttttgtc gttcttgctt tcctcaatga tatttccttt gtcttccaat
     5281 agacctctgc tactttaatc ggatgtattt ggcaacttcc tttacttaat tcaactagta
     5341 gaatagatgt agaagcttgt tcctttttag tatgaccttg aggcgcacct ggataaacaa
     5401 ttgtttgctt ttcatttaag accgttggca catggatatg ccctaaagcc caataatcgt
     5461 atcccttttc ttgcatcttt gagggttgga aaggagcata gtttcctttt tgagagacgc
     5521 caggttcccc gtggtacaac ccaatatggt aatctgtgag ttctctagaa ggaaattcca
     5581 tcactttgtc tttttgaatc cacggttgac gataactaaa tccactgaga gagacagttt
     5641 ctccactttt tatcgttatt ttttttgtct ctaccgtttc ggatgtaaat aaatggacat
     5701 ttttaggaaa ggagaaccaa taccgctctt tttgatagaa atcatgattt ccgaatatta
     5761 taaatacagg tatatggttc ttttctaacc gtttcatctg ttctgaaaaa tgtttctgta
     5821 tttttaatga aggacgattt tgatgaaaat tatctcctgc aagtaaaaca aaatcaacgt
     5881 tatttattat tgcctgttca atgatatttg ataaaacggt cagattcgtc ttcaatagtt
     5941 tttcctgtac agttttatca agagttgtca gtccttcaaa tgaccgatcc atatgtaaat
     6001 ctgccgcatg gataaatttc accattaaaa atttcctcct ttttttcttt tatcatacca
     6061 ttttcaatgt tttttttcat caataaccga aaatttgttc gtattatgca aagaaattac
     6121 gataaatggc cgacttcaaa aaataagttt attcgaaaaa aagattgtga catgattttt
     6181 gtcacaatcc taaatatcta tgttatattc gcttcgtttg acactgaaga agtactattt
     6241 gtactggaat ttatgctcca tttttccgta tttagaattc tatgttctat actaccttaa
     6301 ttagtttata tggtaagaat tctttcaaat gcttgagata ctccgtcttt atcatttgaa
     6361 acagtgacat aatcagcttt tttcatgatt tcttgatgac tatttcccat tgcaaaacta
     6421 atatctgcac tttcaaacat agataagtca tttaaactat ctccgatcgc gattctcaaa
     6481 ggtttcttta aattgtagat tgattccaaa cgatcaaaca ccacaccttt agatacattt
     6541 tgaggtacga tttcaatatt tacaggtgcc gattcagtaa cagacacatt tagcatcttt
     6601 ttcaaatggt taaatatatt agctttcaat tgacgattag gaataaaaat aaaaaatttg
     6661 taaatgctga tagcatcctt ttctataatt tcctcaatat tattataagg gattgaacaa
     6721 atagattttt catactcaag ctcatagttt agctctttga tagaaaattc actccctacc
     6781 tcgtgaaaaa tttttttcat tttattttta taattctgag attcataact tccgagattt
     6841 gtatatattt tggttggaaa tttcgatagg acagaatcaa ttttttttac atcattttta
     6901 tcgaaggaag actcaaatat ctttttttct ccatcaaatc caacagaacc atttaaccct
     6961 actgctggta tccttaaacc agattttttg agaatattat taatgtcttc tacttctctt
     7021 ccagaacaaa tgaatggaaa acagtcttcc ggtaaatgtt tcattgttct tgcatttttt
     7081 tgactaatgc tctggtcaga attcaaaaga gtaccgtcta aatctatacc aattagtttc
     7141 atttcatcca tgtctcctat acgttgatta taaattcact gcttattgta agtctaaatt
     7201 tctcacaaaa catatactaa ttgtaagaaa ttttatatct aattttcaat tatccgcata
     7261 aacactatac attcaagcat tttgaagtat agaaaaacac tcatcttacc acgaatttac
     7321 cacgattgaa tgagccttga tatgaccgta aaactactaa gggagatagc acgaaactgt
     7381 gtatctcccg atttttttat acagttgatt acttcactcc gatttcaaat ttcaatattg
     7441 ctttcatcat gtctgtatga atttctcctt ttagttcttc atcaacctgc ataatgattt
     7501 taccactttc agagtggtaa aaaggacgca agcataattt agaaatataa ccgtcataaa
     7561 acttcaaaat ctctttaact gccatttcat ctccgcttga cgctttgcaa atcaattcaa
     7621 aagagggata gccataatct ttactcattt ctttacttcc cttcgctcaa aagcattttt
     7681 aacttcctta gtcctctagt tcttctactg caaatagcag aacgtgatac atggtacagt
     7741 tctgaaattt ttctgtcgtt cattccttga aaataacgta ataaaatcgc actacgctgt
     7801 ttttctgaca gtctatgtaa aactgtacca agtgtttcat cagatacata aattgtaaag
     7861 tgcaagactt taaatgatac acaatcataa aaacaggtat caatcgttcc acgttcaact
     7921 tgtttcgttt cggctatttc acaaaacaat acttcacgtt tagaatgtct tgcaagctgt
     7981 ctgtttcgac ttttcacttt gttcttaatg acaatcagca ttaaagaatt gaactgtaat
     8041 ctgacgatat gctcgaaaga agatgtcctc ataatctcac cccctttctg ctaatgctga
     8101 aatatataaa cggaataccg tttataccct ttagaaatac agaaaaatgg taagaaaaaa
     8161 gcccacacaa aacatatatt tgtgcgagcc ttaaatactg caatattact ctttctttta
     8221 taacactatc cttttagcag atttcaaatt ttcttttata tattgttttt agcctaacat
     8281 actatttaac cccttgtatt atatctctaa cttttgaaaa attctttttg aatatattga
     8341 aatcatgatt gaagcaatag aagctacaaa cataacaacc catgtttctt gtataaatac
     8401 atcgaataaa ataccgtaaa ccatttgtcc aataggttga ccgcacatag caactgctac
     8461 cagcgtagcc attatctttc ctactaattg cggtggtgtt tgtgtctgaa caagagtgta
     8521 aatttgaaca acaaacatag tagatacacc catagccaca aagcacatca ctgtaactac
     8581 ccagtaggaa actattgcag gcaattctaa aaatagcgaa attcccataa gtaaaacagc
     8641 agtagaattt cacgtttctt gtctggcaac tcacgtaatg cttcactcaa caaatcattt
     8701 tcaacgccta ctgataaccc attgagtgta aaaatctgaa agtcagttga atagttatct
     8761 gttgtcgcaa actggctaac aagataatcg ccaacatccg aaaaggacac ctcacgcttt
     8821 gcaatccttg aaagataaag catataattc tttcgctcgt cttccatagc acgtttacag
     8881 atatagtcaa actgattttc tattgtggtc tgaaaagaag atggtttcat gtttctcacc
     8941 ccctttctgt ctaggaaagg aagtgagcct tgctcgttta tctcctttca ctcttagtcc
     9001 caatgtgaaa gggggatttg ttgcattact gataaataaa ctttgtaaaa aagttctgaa
     9061 tagccaaaaa agcatataaa cagatttatt tctctgttta catgcttctg ttattctatc
     9121 tatatgattt ataaaaccac attggtggac gtacttatct attgcagata gacgactttt
     9181 tttgacaaga acccaatgta aggaaattta ttgtatatga tgtacttcat ggcgacgttg
     9241 acctccaaca aaccgccatt tggaagtaat atacaatatt ttaacagcgt aaatagcact
     9301 accatataac ggtttttttt attggcgttt agtagtgctt tttattaaat ataaacctat
     9361 aaaccatata acacgttttt ctatacctgt ttttaattca gtaggaacaa taaaatgtat
     9421 agaggtggtc tactatgcgt aaaaaagaag ataaatatga ttttagagcc tttggtttag
     9481 ccattaaaga agctcgattg aaacgaggtt taactcgtga acaagtggga gcattgattg
     9541 aaattgaccc acggtactta actaatattg aaaataaagg gcaacacccc agcatacaag
     9601 ttctttatga ccttgtatcg ttacttcatg tttccgttga tgaatttttc ttacctgcta
     9661 ataacttggt aaaaagcacc cgacgattac agatagagaa atacatggat agctttacag
     9721 acaaagaact atccttaatg gaatctttag ccagcggtat caacgaagca agaaacatcg
     9781 aagactaatt aaaagaatcc atacataacg gaaagagccg ataaaatgag attgtattaa
     9841 tctcatttta tcggctctgc gtctttgcgt ctggctctgt aatcacagtt actttgaact
     9901 gctttatttc aattaaattt tcttgtctgc atttcggaca atagaggggg aattttttta
     9961 attcagtatc ttcccttatc tttaatcgtg ttttatttcc acatacagga cacaatatcc
    10021 acttgtagtt tataataact atctcctcct ttacacttta attcaaatct ttattaaaaa
    10081 atatttcatc ttatttaaca agaaaccata tttatataac aacataaaat acactaagtt
    10141 attttattga acatatatcg tactttatct atccgactat ttggacgacg gggctggcaa
    10201 acaggttcac cggtagtaac atggtaccct tttaactctg ttaaacaaac actacgtcca
    10261 tttgtaaaga aagttaaatc actacgatat tcttgaatac accgagcagg gatttctcca
    10321 ctaagaatga cctcattatt tttcaattga gtgtctacga tgttcgcaca atatttagga
    10381 gcatcgttgt atgctcgtga aagatattcc tgtggcgcat aaattttaaa actaagatat
    10441 ggctctaaca attctgttcc agcttttttt aagacttgtt ccaatacaat aggagcaagc
    10501 atccgaaaat ctgctggggt actaacaggg ctatagtata agccatactt aaaacagatt
    10561 ttacagtccg tcacattcca accatacaat ccttgttcac agccatagcg tatcccctcc
    10621 ataactgcat tttgaaacga ttgatttaag tatccaagag aaaccgagct ctcatactgt
    10681 actccgctcc ctaatggaag ctgtgctaca gatagaccaa tggaagccca gaaaggattc
    10741 ggtggaactt cgatgtgaat ggtatactct gcttttttta acggtctttc catataaatg
    10801 actgtaggct cttttatttc tatctccaca tgatactttt cttgcaacag tgcactaatc
    10861 acttccattt gtactttccc taagaaagaa agtataattt catgtgtcgt agaatccacg
    10921 taatatcgta gaagcggatc actatctgag atttccaaaa gggcatcaag caacatttct
    10981 ctctgttcag gtttactcgg ttcaacagtt gtttgtagta gagggtgcgg attttcaatc
    11041 ttttttctct gtggcaatag ttttgtatct ccaagaacac tatctaactt caaaaactca
    11101 ttttgcaaaa taacaatttc cccggaataa gccttatcga ttttacataa ttcaccattt
    11161 attgaagtat acatttctgt aatttttatt ttttcctttt ccgatattct aaccgaatct
    11221 cgcaaatgca gtacgccact ataaagacgt atatatgcaa gacgctgtct tttttccgaa
    11281 tactcaattt tgaaaacttt tccgcaaagt tcagactgac ctcgatgtgt tgatgaataa
    11341 aatttattcg taatcacttc tataaggtta tcaatcccta tattgttttt tgcacttccg
    11401 tgataaacag ggaacaggga acaattatga aatcttatgc tttcctcttg ttcgagttct
    11461 aatgcttcca ataatttccc agacgtatat ttctctaaaa ggtcatcgtt tccctctatt
    11521 accgtatccc attgttcaga ttcggtaaag ttcgtcacac acatattagg atacagttct
    11581 accttctgtt tgattacaat ttcggcagaa agtttctctt taatatcctg ataaaccgtt
    11641 gataaatcaa ttccattttg gtcaatctta ttgataaaaa agattgtggg aatccccatt
    11701 ttcctaagtg catgaaataa tatacgagtt tgtgcttgta cgccatcttt tgcagaaatc
    11761 agtagaattg ccccatctaa aactgataat gaacgatata cttctgctaa gaaatccata
    11821 tgtcctggcg tgtctatgat gttcaccttc gtattttccc actgaaaaga ggttattcct
    11881 gtctgaattg taattcctct ctgacgttct aaaagcgtat tatccgtcct cgttgtacct
    11941 ttgtccacgc ttcctaattc tgtaatcgct ccactgttat ataataagct
//
LOCUS       DAJSBM010000073        11307 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJSBM010000073 DAJSBM010000000
VERSION     DAJSBM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.074"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..1819)
                     /locus_tag="ONT67_002331"
     CDS             complement(83..1819)
                     /locus_tag="ONT67_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCR2356051.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1816..2520)
                     /locus_tag="ONT67_002332"
     CDS             complement(1816..2520)
                     /locus_tag="ONT67_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2356052.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2651..3154
                     /locus_tag="ONT67_002333"
     CDS             2651..3154
                     /locus_tag="ONT67_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCR2356053.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3114..3452
                     /locus_tag="ONT67_002334"
     CDS             3114..3452
                     /locus_tag="ONT67_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCR2356054.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3473..4891
                     /gene="ffh"
                     /locus_tag="ONT67_002335"
     CDS             3473..4891
                     /gene="ffh"
                     /locus_tag="ONT67_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR2356055.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5003..5581
                     /locus_tag="ONT67_002336"
     CDS             5003..5581
                     /locus_tag="ONT67_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR2356056.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5585..6106
                     /locus_tag="ONT67_002337"
     CDS             5585..6106
                     /locus_tag="ONT67_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2356057.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6185..6739)
                     /locus_tag="ONT67_002338"
     CDS             complement(6185..6739)
                     /locus_tag="ONT67_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2356058.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT67_002339"
     CDS             6992..7267
                     /gene="rpsP"
                     /locus_tag="ONT67_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR2356059.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7279..7530
                     /locus_tag="ONT67_002340"
     CDS             7279..7530
                     /locus_tag="ONT67_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCR2356060.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7655..8446
                     /locus_tag="ONT67_002341"
     CDS             7655..8446
                     /locus_tag="ONT67_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356061.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8616..9137
                     /gene="rimM"
                     /locus_tag="ONT67_002342"
     CDS             8616..9137
                     /gene="rimM"
                     /locus_tag="ONT67_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR2356062.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9127..9888
                     /gene="trmD"
                     /locus_tag="ONT67_002343"
     CDS             9127..9888
                     /gene="trmD"
                     /locus_tag="ONT67_002343"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR2356063.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10024..10371
                     /gene="rplS"
                     /locus_tag="ONT67_002344"
     CDS             10024..10371
                     /gene="rplS"
                     /locus_tag="ONT67_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR2356064.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10503..11264)
                     /locus_tag="ONT67_002345"
     CDS             complement(10503..11264)
                     /locus_tag="ONT67_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2356065.1"
                     /translation="MIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKNPINEVVIDY
                     KRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGEAIALSKDDV
                     HITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNP
                     NGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYA
                     IQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
BASE COUNT         3705 a         2072 c         2291 g         3239 t
ORIGIN      
        1 agtaaaaaag aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa
       61 acgcacgagc agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg
      121 ttccaagatg actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa
      181 tcgctaaccc taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa
      241 aacgctcaaa gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag
      301 aaaacgatag atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat
      361 attggacagc attttcaatt agatttttga taattggata aaggatatct gttttagtag
      421 taaatggttc gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct
      481 gataattttg aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag
      541 aattctcgcc atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg
      601 catctttttg catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat
      661 ccagtaaagt ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga
      721 cgttgccaac gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg
      781 ccacccctag aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta
      841 atgtctgccg accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca
      901 cttgatgaat cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag
      961 gttgatagct taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag
     1021 aatcagcatc atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt
     1081 gctcttctgt ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa
     1141 tcgtctgata caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag
     1201 gattttgcac catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat
     1261 agatcagata atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga
     1321 cccaacggcg aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta
     1381 aaatggcttc taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat
     1441 tcaaagtagt acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga
     1501 tttcgggacg ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct
     1561 ttccagtagc atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag
     1621 cagttttgtt ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg
     1681 ctttttttgt caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa
     1741 tcaattgcca gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat
     1801 aaattttttt ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt
     1861 caccaagtac tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg
     1921 tacatctacg attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt
     1981 gtctcggtcg atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt
     2041 cggtgttagt tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt
     2101 gatttgtcca atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc
     2161 aattcgtctg aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt
     2221 caaatagtcg tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc
     2281 cgtcagcatc aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat
     2341 gccatccatc gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc
     2401 taattctaat ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa
     2461 attgaacgtc aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat
     2521 tcaaatctcc tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag
     2581 ggctatttta caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga
     2641 agagcagtca atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg
     2701 acagaaaaaa gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt
     2761 ctgtccagaa gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg
     2821 aaatggattc gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt
     2881 aacggaagcg tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat
     2941 tgatctagtc atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg
     3001 aagtttttcc ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca
     3061 aaaaatgact gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa
     3121 tcgaaaagac caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa
     3181 aacaaatgaa ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg
     3241 aagaatacga agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct
     3301 tggaggatta tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat
     3361 tggaaaatat gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat
     3421 atgtacagca aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt
     3481 tgaaagctta actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt
     3541 atcagaagct gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga
     3601 tgttaacttg caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc
     3661 agaagtgctg gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt
     3721 gacgatcaca cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt
     3781 tatcatgatg gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa
     3841 ttatttaaag aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc
     3901 agctgcgatc gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat
     3961 gggaactgaa gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa
     4021 gaaaaacgac tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat
     4081 ggatgagttg aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga
     4141 tgccatgact ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat
     4201 tacaggggtc gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat
     4261 ccgctcagtg acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt
     4321 agaagtattc cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac
     4381 attgattgaa aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa
     4441 aatgcgggaa aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg
     4501 tatgggccca ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat
     4561 cgaaaacgtc aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat
     4621 gacgccagca gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc
     4681 tgccggttct ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc
     4741 aagaaaaatg atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat
     4801 gtttggttca ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa
     4861 aaataagaag aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg
     4921 aaaatgtcta ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac
     4981 tctaaacgaa gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg
     5041 gcaatatcat gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa
     5101 attgatgctt gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga
     5161 agcattcgat gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac
     5221 aaaaatcagt gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc
     5281 agccgtccaa aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga
     5341 gtatctttgg tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga
     5401 agtcccaaca acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt
     5461 taaatttatc ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga
     5521 tcaccttgac agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata
     5581 aagcatgcga aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa
     5641 aacggatgat cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc
     5701 tttttatgaa acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa
     5761 atatttggag accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc
     5821 acaattacaa ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa
     5881 tctacaaatg acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc
     5941 gctattggaa catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg
     6001 ggacgggatt cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac
     6061 gacacgattc ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata
     6121 aaaaaggcaa ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg
     6181 ctttttactc tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga
     6241 aacttaatgt tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga
     6301 tatatgcttc cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat
     6361 ggtggtttgc gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt
     6421 tatgatccca aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata
     6481 ctgctggcac agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca
     6541 cttcctcacg aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat
     6601 tgtaaaatgg cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt
     6661 tcgcaaaagc ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa
     6721 atccaatttt ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa
     6781 aagataagta actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat
     6841 tacttatttt taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt
     6901 aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt
     6961 gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat
     7021 gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga
     7081 tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt
     7141 tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt
     7201 acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa
     7261 gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg
     7321 ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag
     7381 tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt
     7441 gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt
     7501 ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg
     7561 cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa
     7621 gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc
     7681 atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct
     7741 atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc
     7801 caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt
     7861 gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct
     7921 caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg
     7981 tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata
     8041 tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga
     8101 cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc
     8161 aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa
     8221 tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa
     8281 cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa
     8341 tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga
     8401 tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga
     8461 tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt
     8521 ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta
     8581 aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa
     8641 aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc
     8701 cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg
     8761 tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag
     8821 gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag
     8881 acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg
     8941 tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg
     9001 atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg
     9061 tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga
     9121 ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg
     9181 gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc
     9241 cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca
     9301 ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca
     9361 ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa
     9421 atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga
     9481 tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt
     9541 actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat
     9601 gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat
     9661 ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat
     9721 ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa
     9781 cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa
     9841 gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa
     9901 tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa
     9961 ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa
    10021 accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg
    10081 ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt
    10141 atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat
    10201 acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca
    10261 cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac
    10321 ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta
    10381 tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct gtttttttat
    10441 attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa aggggcaaca
    10501 agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt cacatgcaaa
    10561 taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga
    10621 ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt
    10681 tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt
    10741 tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt taattctaga
    10801 agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca
    10861 gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc
    10921 ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg
    10981 attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt
    11041 ttattgtgtg aagttgtgaa atttactagt cttttatatt catcatcttc taaaaactta
    11101 tctttaattt ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga
    11161 tttttttcta catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta
    11221 attacactga cgtacttatt tgacaagtca tctttatata tcatatcttc aaaaatatta
    11281 tttaaatcta ctgtattaat accagaa
//
LOCUS       DAJSBM010000074        11262 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJSBM010000074 DAJSBM010000000
VERSION     DAJSBM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.075"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            224..1483
                     /gene="lysA"
                     /locus_tag="ONT67_002346"
     CDS             224..1483
                     /gene="lysA"
                     /locus_tag="ONT67_002346"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR2356066.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1470..2270
                     /gene="dapF"
                     /locus_tag="ONT67_002347"
     CDS             1470..2270
                     /gene="dapF"
                     /locus_tag="ONT67_002347"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR2356067.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2273..3154
                     /locus_tag="ONT67_002348"
     CDS             2273..3154
                     /locus_tag="ONT67_002348"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR2356068.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3151..3867
                     /locus_tag="ONT67_002349"
     CDS             3151..3867
                     /locus_tag="ONT67_002349"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR2356069.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3873..4568
                     /gene="dapD"
                     /locus_tag="ONT67_002350"
     CDS             3873..4568
                     /gene="dapD"
                     /locus_tag="ONT67_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCR2356070.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4584..5585
                     /locus_tag="ONT67_002351"
     CDS             4584..5585
                     /locus_tag="ONT67_002351"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR2356071.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5582..6943
                     /locus_tag="ONT67_002352"
     CDS             5582..6943
                     /locus_tag="ONT67_002352"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCR2356072.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6903..8054
                     /locus_tag="ONT67_002353"
     CDS             6903..8054
                     /locus_tag="ONT67_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR2356073.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8029..9198
                     /locus_tag="ONT67_002354"
     CDS             8029..9198
                     /locus_tag="ONT67_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2356074.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9373..10752
                     /locus_tag="ONT67_002355"
     CDS             9373..10752
                     /locus_tag="ONT67_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2356075.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11033..11262
                     /locus_tag="ONT67_002356"
                     /pseudo
     CDS             11033..11262
                     /locus_tag="ONT67_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3748 a         1959 c         2509 g         3046 t
ORIGIN      
        1 catcactaaa tagtgttgaa tttatgtttt tatgtataaa tagcttctaa tagttcgggt
       61 attttgtttt tataaccatt tttattctta tttttaaaaa aatatttcga aatatactag
      121 acaattcaga aaattttgtt taatattatt ccaataaaat tcaaatcttt atcaaatgaa
      181 attccaataa aaacggaaaa aaagtgaaaa gaggaatgaa aaaatgacag aattgataat
      241 tggcggagtc acagcaagca cattggcaga aaagtataag acacctctgt atgtgtatga
      301 tgaagaaaag atgagacaaa cgatgctgtc attcaagcga ggctttgtat cgaaacgttt
      361 tgatacaaaa gtgctttacg cctctaaagc attccaaacg attgagatgc tgcatttagt
      421 tcgtgaatat ggattcgggc tagatgttgt cagtggtggg gaaatctata ccgctttgca
      481 agctgatttt cctagagata ctatctactt ccatggtaac aacaagacgc cagaggaact
      541 gatctatgca cttgaaaatg atttgttgca tattgttgcc gataatttga tggaagttga
      601 attattagca gaattaagcc aacgctattt aaaaaaaatc tgtatcatgc ttcggttgaa
      661 tgtcggagta gaagcacata ctcatgaata tatcgtcact gcgcatattg attcaaaatt
      721 cggcatgagc tatgaaagta aagagtgcca acaagccctt cagttgataa aagaaagcag
      781 ctatctagag ttagaaggat ttcatgctca tatcgggtca caaatttttg atgttactgc
      841 ttggttagct gaaatcgaca aattgacgaa ttacttaacc gattttgacc aacctctttc
      901 cttgaatctt ggcggagggt ttgggattcg ttatacacaa aatgatcagc cgatcccagt
      961 cgaagaggca ctaaaaaaac tgattgaata tacggaagaa gctttggaaa aacgaaaact
     1021 gaccattcgt caattactga tcgaaccggg tagaagtatt gttggagaag ctggtacaac
     1081 actatacacg attggtttca tcaaaaagac accacataaa aaatattact ttgtagatgg
     1141 cgggatgaca gataatatcc gtccagctct gtatcaggca gaatacgatt gtgatttagc
     1201 agcaaaactg tcggaagaaa aaacagagaa agtgacgatt gcaggaaaaa tgtgtgaatc
     1261 aggcgatgtg gtaattaaag aagcttattt gccgaaagca gaatcgggtg atcttttagc
     1321 tgtttattcc acgggagctt atggatactc gatgagcagc aactacaatc gagcgacacg
     1381 accagcagtt gtttttgtca ataatggcca atcaagactg attgttcgca gacaagcatt
     1441 ttctgatcta ctaagggggg aagtttccta tgatccttca taaatataat ggctgcggaa
     1501 atgattttat cttattagat tacgcagaag acacagatta tagtcaatta gccgcttctt
     1561 tatgcacaaa agaaaaatat gatacagacg gattgatcgc cgtaaaaatc gatccgttgg
     1621 aaatgatcta ttacaacaaa gatggcagcc gcgcgccgat gtgcggaaat ggcattcgct
     1681 gttttgctcg atacgtaaat gatcagaaca tgatgaatga acaggaattc aacgtagaga
     1741 cattagcagg cacaagaaaa attacggtgt tggatccctc acctttttat tgcagcgtag
     1801 atatgggagt gccagatgat tctccggaaa aaatcaaagc taatcaaact tatccaataa
     1861 aaaaccagga aatagagatc aatggtgaaa aagtaacgat cactagttta tttttaggaa
     1921 cgattcatac agttgttttt gttgaaaatg cgcgtgagga agtctggaga aaaagaggtg
     1981 aactgatctg tcatcatcct ttgttcaaag aaaaaaccaa tgtcaacttt gtagaagtag
     2041 tcaatgaaca agaattgatc gtccgtacct atgaaagagg agtaggttgg acacttgctt
     2101 gcggtacagg ttgctgtgcc tcttatgtgg ttgctaagga aactgggaaa atttcagcag
     2161 atcaggtagt tgttcacttg gaacaaggag acttgatgat ctctggtgag gatgcgatac
     2221 aaatggctgg cccagcagtt catgaatgga caagaatatt ggaggaatga agatgataaa
     2281 aggttcgatc gtggcgctga tcacgccgat gaatgaagag ggatcagtag attatgctgg
     2341 tttggaaaaa ttgatccaat tccatttaga tgaacaaaca gatggattat tagttttagg
     2401 tacaacagga gaatcttcta cgctcacaca atctgaggag gagcagatcc ttcaactgac
     2461 agtaaagaaa gtggctggcc gtgtgccggt gattgcagga gctggaacga ataatacgaa
     2521 agaaacgatt gaaaaagcga agcattttgc ttctttgggg gcagacgcac ttttggtcat
     2581 caccccttac tacaacaaaa caagtgatgc aggactagct gctcatttta cagcaattgc
     2641 agaagcctca ccgattccgc ttattttgta taatgttcct tctagaacag gcatgtcgat
     2701 tccaatccat gtattagtaa atttagcaga acatcctaat atcattggtt tgaaagaagc
     2761 ctcaggagat atggcttatg tgatggatgc cgctcggttg attggagaag aattcttcct
     2821 ttacagtgga aatgatgatt tgatcctacc agtaatgagt gtaggaggaa gcggtgtaat
     2881 aagtgtatgg gcgaatatcc aacctaaaat agtccatgaa ttagtaaaag atacgcagga
     2941 cggaagatgg cagcaagcaa aagaaaagca attaaatgca cttgaactga tccatgcctt
     3001 gttcagtgag accaatccta ttcctgtcaa agcagccatg tcattattag acttaccttc
     3061 cggacctcta cgcctgcctt tagtttctct ttcaaaagag aaaaagaaac aattagctca
     3121 gcttttgtta aaagaaagga cggttgaaaa atgaaaatcg gactgattgg cagtggacag
     3181 atgggaaata gagtcgaaga agttgcacaa gaaagaggag atacggtcct tcttttacgg
     3241 acggcaccta aagaagcaac tacgcttgct tttacggaac tgccagaact gcttattgat
     3301 ttttctcatc cagataattt agagaaaatc ctttcgtata gcttatcata tcaacttccg
     3361 ttagtcattg gcacgacagg ttatacacaa tcacagttcc aagaaataaa agaacatgcc
     3421 aaacatgtac ctgtgatgta tagtgctaat ttttcgttcg gcatcatggt gatgaatcaa
     3481 ctaatcaagc aagcagctgc aatgttgcaa ggctggcaga tcgaactgat tgaaaaacat
     3541 catagtcgta aaaaagatgc accatctggt actgcaaata tgctgcttca aacaatccaa
     3601 gaagtgcaaa agctgcagcc cgtatacaac tggcaaggga aaaaaagaga agaaaaccaa
     3661 attggtgtac acagtattcg tgcaggttct ttacctggtg aacatgatgt attgtttgct
     3721 gctactgatg aaatatttac gatcaaacat gaatcttttt ctaatcgaat ctttgctgca
     3781 ggcgcagtag aagcagcgga ctggttgaaa gatcagtcac caggttatta caaattagaa
     3841 gatttattaa tggacaaagg ggtgtaaaaa acgtggaagc tcaagaaatc atccgctaca
     3901 ttcaaacggc atctaaaaag acgcctgtga aagtctatct gaatgtaacc gagccaatcg
     3961 aatttaaaga aagtaaagtt tttggtgaag gaagcagctt cgttgttttt ggtgactatg
     4021 agacgattgc tccggtttta gaagctgaat cagaaaaaat cattgaaatc gaaatcgaaa
     4081 ctgctgcaag atacagcgca gttcctttgc tagatattaa aaaaattaat gctcgtatcg
     4141 aaccaggtgc tattatcaga gatcaagtgt caatcggcaa caatgccgtg atcatgatgg
     4201 gagcgattat caatattggt gcagtaatcg gtgaaaacac gatgattgat atgggtgctg
     4261 tcttaggtgg acgagcgaca gtgggcaaaa actgccatat tggtgcagga gctgtcttag
     4321 caggtgtgat cgaaccagca tcggccaaac cggttatcgt agaagacggc gtactggtag
     4381 gtgcgaatgc agtgattgtt gaaggtgtcc atatcggaaa agatgcagtc gtggcagcag
     4441 gagcagtggt tttagaagat gtggcagcag aaactgttgt cggtggaata cctgcacgtg
     4501 tattaaaaat aagtgatgat cagacaaaag aaaatacagc attgattgct gcgttacgcg
     4561 aattatagag gagatggaga aaaatgcgcg aaatagatac ctgtattgtg ggggcaaccg
     4621 gattagtcgg ccaaacgatg ctcaaagtgt tagaagaata tgattttcca gtaggacgat
     4681 tgaaattatt agcttcaaaa cgttcagccg gtaaagtttg tgtattcaag aaaaagaaac
     4741 acactgtaca ggaattgaca gaaacttcat ttgaaggata tgatttagcg ctattttctg
     4801 caggtgcttc tgtatccaaa gagtttgcgc caatcgcacg aagtcaggga ctatttgtca
     4861 tagataattc ctcagcatgg cgggaagatt cggatattcc attaatcgtt ccagaggtca
     4921 atctggaaga ctatacattg aatcggctga ttgcaaatcc aaattgttca accattcaag
     4981 cggtacttcc gcttaaagct ttacaagaag cctttggtat ccagcgagtc aattattcga
     5041 cctatcaagc agtttctggt tccgggcaaa aaggcattga agatcttcat cgtacgcaaa
     5101 atgacgagga accagttttg tatccttata atattagcca aacggtgatt ccggaaatcg
     5161 atcagccttt ggaaaatggg tatacaaaag aagaacagaa aatgataaat gagacccgta
     5221 agattctgca tcagcctgct ttgccagtat cagcaacctg cgtacgtgtg ccaatcgaaa
     5281 atggacatgg agtctctgtg gcagttcaac tgcaaaaaga gtttacgacg gaagaaatta
     5341 gagagtgtct cagccagttt ccagggattc aattagaaga tgatctagca gcacataaat
     5401 atccgacgag cattcttgct agaggaacag atatggttta tgttgggagg atcagaaaag
     5461 acacttcttt agagaacgga ttgttgttct atacgactgc cgataacata cgcaaaggag
     5521 ccgctgcgaa tgccgtacag attgctgcag ctttaatgca gaaaatagcg gaggtggcaa
     5581 gatgagtaat acgctgaaag tagcgaaatt cggcggaaca tcgatgtctc atgaagagca
     5641 attcaaaaaa gtccgtacaa ttgttctttc agatcccaat cgccaagtga tcgttgtttc
     5701 tgctttaggc aaacgtacgt cagatgatga caaagtaaca gacttacttt atttgattta
     5761 tgcttacctt cagcatcatg ttgattggac acccctttgg aaaaggattt gtgatcgttt
     5821 cattcaagta agagatgttc ttgccctgca gttacctatt gaagatgaac tagcacgcat
     5881 acagaaacaa ctggaagacg aaaagataac tgaagattat ttagtaagta gaggtgaata
     5941 tctgactgct caactgatgg ctgagtatct tggttaccag tttattgacg caaaagacct
     6001 gattttcttt gactatgatg ggaaaatcga tcaggaaaaa acagcacagg aaatcacaaa
     6061 agcatttaaa agatatccga aaatggttgt tccagggttt tacggggcga accctgcagg
     6121 aaaagaaaaa ttattgggcc gtgggggaag cgacattacc ggcgcgatac tggcgaagat
     6181 gttgcaagcg gaaagttatg agaattggac ggatgtttca ggaatcatga tggctgatcc
     6241 gcgaatcatc gatcatccta aaatcatcga ggaaatctct tttagagaac tgagagagat
     6301 ggcttatatg ggggctaacg ttcttcatga agcagctgtt ttcccagttc aagaggctga
     6361 tatccccatc caaataaaaa ataccaatga tcctttcgct caagggacaa aaatatcgaa
     6421 tcacgaaata gaaaaggaaa atggactgac aggaatagct ggacgcaagg attttctttc
     6481 catcacttta ttcaaacgtc atatgtccga tgaaatcggg tttatctgga aagcaatgag
     6541 catttttgct aaacatgaga tatcaatcga acatattcct tcagggatcg acaatatcgg
     6601 agtgatcgtc tcagcagaag cgattgccga taagttattt ctcatcacga aagaattaaa
     6661 agaaacactt ggcgtggaag aaatcgtcgt gatcgaagac ttagctttga tctctgtcgt
     6721 tgggggtccg cataaagaat tgattggatt atctgggaaa gtgttatcga tattgaacaa
     6781 attagaaatt cgcacatcga tcttatcaca aggagcacag gagttgaatc tgatcatcgg
     6841 tgtaccgaat aatcaatatg aaacagtagt gaagggtatc tatgaaggga tggtgaatac
     6901 ggatgctgaa agaatatcga atggcactcc atcaaattcc tgaaatcggg tttcatgaat
     6961 tcaagacaaa aaaatattta tatgaacaag taaaaaacag cggggccgtt atccatgaaa
     7021 tcggcgaaac aggactactc ctttattttg ataatcagca agaagaaacg attgcttttc
     7081 gaacagatat cgatgcactg ccagtcaaag aagaaacgaa tctctctttt gcttctaagc
     7141 atgaaggtta tatgcatgca tgtggtcatg acggacatat ggcgatgctt ttagggttga
     7201 ctgactttat aactaaacac attgaggatc ttatttataa tgtagtactg atctatcagc
     7261 catcagaaga aatttccggt ggcgcacaaa gcgttattca gtcaggattg ttggataaat
     7321 atgaagtaca agcaatattt ggctttcatc tatggcctgg actagctaag ggagaagttt
     7381 tctctcgacc aggacctttg atggcacaaa gcagtgaaac agacattatc attaaaggcc
     7441 agtctgcaca tattgcttct agtgaaaaag gaatcgatag tctggaggca gctgtacgat
     7501 ttttgaagaa tgtttatgat tttgaccggc aattaccggc agagtggaat catctaatga
     7561 aatttggcca aatccaaggt ggaacgatcc gcaatgtttt agctggagaa gttattgtat
     7621 ccggaagtat ccgttcttac agttcagata tgcaaaatta tttgaagagg ggattagacc
     7681 atttagcaga acagtttcac tctacagcta ctatttcttt tcgctataat gacggctatc
     7741 cagctgtaat caaccataga ggcttatatg cagcactgtt agattcaaac aaactacatg
     7801 aattagatgc tccagttctt caagccgaag attttggcat atatacgcaa caatatccat
     7861 gtgtattttt ctttttaggt gtcggtgata cccctgcttt acatgatccg acgttcgatt
     7921 ttgatatgga tgttttagaa aatggtgtag aatggtacaa aacgattctt tttacagaaa
     7981 atcttcttgt aaaaatcgat agcagttcaa aagaggagcg agtaaataat gatgaattta
     8041 tccaatcgat ttagtcctag gctttctaga atagaagtat caaaaatacg acaattcgat
     8101 cagcaaatat cgtcgatacc agatgtgatc aaactcaccc tgggagaacc ggattttcca
     8161 actcctgaac atgtcaagca agcaggaata gcagcgattg aagaagactt ttctcactat
     8221 accggaatga gaggattaga ggaactgcgg gaagcggctt gtatatttca gcaacagcgt
     8281 tatggattaa cttatgatcc gcaaacggaa gttttgacga cagttggtgc aacggaagcg
     8341 attgcctctg cgttgttatc tgttttggaa gaaggagaca aggttcttat tccagcgcct
     8401 gcttattcag gatatcagcc attagtggaa ctagctggtg ctgaattgat cccgatagat
     8461 acttctgaca cagggtttgt ctgtcagccg gaacaatttg aacgagcatt tgaacaatat
     8521 ggatcagctg tcaaagcagt cattttaaac tatcctaaca atcctactgg gacgacattg
     8581 tctgcaaaac aattgaaagc aatcgctgag gttttaaaaa aatatccggt atttgttata
     8641 agtgatgaag tatatgctga attgacttat agtggaacgc atatgtccat agctacttat
     8701 cttccagaac aaacaatcgt gataagcggg ttatccaagt cacatgcgat gactggttgg
     8761 cgagtgggct ttatttttgc tcaaaaagcc cttattgacg aactaattaa agtccatcaa
     8821 tatctggtga ctgccgctac aacgatgtcg caaaaagctg cggctgaagc gctaataaat
     8881 ggggtgaaag atagcgagaa tatgaaagag caatatcaaa cacgaagaga ctatctggtc
     8941 cagcaattgg cgcctttagg atttgaagtg acccagccaa acggtgcatt ttacttgttt
     9001 tgcaaactcc cagctgccat tcaaactgac tcttggcagt tttgtgtaga attagcggaa
     9061 caggcaaaag ttgcgtgtat cccaggaatt gcatttggcc cagaaggaga aggttatatc
     9121 cggatttctt atgctagcag catggaaaaa ttacatgagg cctgcaaacg gatcaaagac
     9181 tttttaacag aacagtgatt catagatttt atcaaaaaaa caatcgttaa ttttgcggta
     9241 tattctgcaa ggagacgatt gttttttaaa tttagttcaa atgaacgcgt ttcttttgat
     9301 tcataactaa tgtaatgtta agtttaaatt gtttagaatg attttaaaag aatattcaag
     9361 gaggtttcat caatggaaaa aatcaaagtt gtcattgtag gtgcatctca tggagggcat
     9421 caatcgatct tggaactatt gacacgatac ggtgaaaatg tagatatcac gctatttgaa
     9481 gcaggagact atatttcctt catgtcatgt ggtatggagc tgtatttaga agacaaggta
     9541 acagatgtaa atgatgtacg taatttcaga ccagaaaact ttccgcaatc caatgttcat
     9601 attttaaata atcacgaagt aaaaacgatc gatgccgata aaaaaacagt aacggttact
     9661 agcgcatcag ataacgaaac aaaagaatat ccttatgaca aattgatttt gagttctggt
     9721 gtcaaaccaa acaatctgcc agtaccagga gcagatctag aaaatgtcta cttgatgcgt
     9781 ggatacaatt gggcaaccaa aatcaaagaa aaattaaatg atccggctat taaaaaaatc
     9841 gctgtcgtag gtgcgggtta cattgggatc gaagcagcag aagcaagccg aaaagctggg
     9901 aaagaagtcg tattgcttga tgtgatcgat agacctttag gcacttattt agatgctgag
     9961 atgacagata ttttagaaca acacctaaat gaaaacggta ttgaagtttt gacaaatgca
    10021 aaaatcgaag cttttactgg taacggaaaa gttgaggcaa tcaaaacaag tgaaaaagaa
    10081 atcccagcag atctagtcat tcaagctgcc ggtgtgaagg caaatacaga atggttaaaa
    10141 gggatcgtcg acttagatga acgtggctgg atccaaacaa atgaatactt gcaaacgaat
    10201 cttccagatg tgtatgcagt aggagatgct acacttgctt attctatccc agcacgcaaa
    10261 aaaatgccga tcgctttagc gactgttgca cgtagagaag cacgttatgt agtaaaacat
    10321 ctttttgaag aagtaccaaa caaaccgttc ggcggagtcg taggttcctc tgcattaagt
    10381 gtctttgact atcattttgc agcaagcgga ttgaatagct ttacagcagc aaaagcagga
    10441 gtagatgtac gagtgtctta ttatgaagat acgttacgac caaaatatgt accagaagaa
    10501 caaggaaatc caaaagtgtc catccaatta ttctcggatc caatgaccca tcaattgcta
    10561 ggaggagcag tattatctac ctatgatgtt acaggtcaag gaaatgtatt ggcactagcg
    10621 atccaaaaca aactgacttt agaagaacta gcagaagctg acttcttctt ccagccaggc
    10681 tttgaccgac aatggagcat gttgaatcta gccgctcaac aagcgttagg agaagaacca
    10741 tttgtagaat aagaaaacga aatatttgaa aagaggttgt gaaaaagctg ccttgcatca
    10801 agtaataaga acagccaagt aaaaacagct tcttatgttt ttaacttggc tggacttatt
    10861 accgcagatg caagcttttg aacaccgttt attttcaaaa aagggactgt gacacgattt
    10921 gtgtaccgac ccccaaaagt tagaccaaaa aatctaactt ttggaggtcg gtatttttta
    10981 tggttaaata tacttttgaa cttaaattaa agatcgttca tgattatttg gatggcaaag
    11041 gcgattggac cgtaaaaatc cagatcaaga gattgaagcg gaaatgctta aaattcggga
    11101 gaagcacaag gattatggct gtttacggat gaccaatgaa ctaagaaacc gcggtttctc
    11161 tatcaataaa aagaaagttc agcgcttgat caagaagcta ggaataaaag taacggcata
    11221 cacgcgtaaa tcacgtcgct acaattctta tcgaggaaaa gt
//
LOCUS       DAJSBM010000075        10973 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJSBM010000075 DAJSBM010000000
VERSION     DAJSBM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.076"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1183)
                     /locus_tag="ONT67_002357"
     CDS             complement(<1..1183)
                     /locus_tag="ONT67_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356076.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QK"
     gene            complement(1170..3038)
                     /locus_tag="ONT67_002358"
     CDS             complement(1170..3038)
                     /locus_tag="ONT67_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356077.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            3004..4641
                     /locus_tag="ONT67_002359"
     CDS             3004..4641
                     /locus_tag="ONT67_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356078.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(4786..5526)
                     /gene="truA"
                     /locus_tag="ONT67_002360"
     CDS             complement(4786..5526)
                     /gene="truA"
                     /locus_tag="ONT67_002360"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR2356079.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(5717..6352)
                     /locus_tag="ONT67_002361"
     CDS             complement(5717..6352)
                     /locus_tag="ONT67_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCR2356080.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(6499..7740)
                     /gene="sstT"
                     /locus_tag="ONT67_002362"
     CDS             complement(6499..7740)
                     /gene="sstT"
                     /locus_tag="ONT67_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR2356081.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            7938..8936
                     /locus_tag="ONT67_002363"
     CDS             7938..8936
                     /locus_tag="ONT67_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356082.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8949..10967
                     /locus_tag="ONT67_002364"
     CDS             8949..10967
                     /locus_tag="ONT67_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR2356083.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
BASE COUNT         3674 a         1972 c         1677 g         3650 t
ORIGIN      
        1 ttttttgatt gaaatttttt ttactagaaa cagttaaagt gattaataac aaaacagtca
       61 taaataaata aaatagttct cttgtcttat tgtatcttct atttttgaaa aatatcatcg
      121 ttaatatcag agaagagaat attatttgca agcttaagat ccgccaagga aattgtaaca
      181 atttggctgg cgtattttgc agaagatacc atggcaccaa actagttgta gctagccaag
      241 tcaaacacgt accataataa atcttttttt ctatgtttgt taatttttta aataaaatca
      301 ggggaataca taaagataac agaccaacta gtcccattga gtaagtagtt aagtcgttgt
      361 tcatagcttc agtcagacta tcgaacacat tagatgcatg agcatataaa tttgtcacgc
      421 taggtttgtt gattttttgg tagagcattt gttcaaacat cggataccaa aaagcactcg
      481 tcagaaccaa tgtcattcct gccgcactaa ataatttttt aaatatagca ataaaatctg
      541 cattcttgaa acctttagaa accaaacgaa taaaaataaa aatgataatt aatgctgtac
      601 tcattaatgt tgatagtaca tggctgtaca ctagcaaact catactcaat gctagcttta
      661 cagacggata cttttcttct tttccagaga tgatttgata agcataaaat aatacaggga
      721 taacaagtgt tattgcgata gcttcggcaa ttgcggaacg atagtagatg tctactgtcc
      781 gataagcaga gaaaatatag agacaactaa ataaaaatgc cgcttttttt tgttttaaaa
      841 accgctcgcc ataatgataa gctacgagtg ttgtacacaa gttcaataac caaacgtaaa
      901 taatatagct tacaatcaaa ttttttgtca tccaatataa cattacagct ggaaaaaaag
      961 tcagaaaggg ataaaaataa tttacaccat atccagtata attgaatgtc tcataattaa
     1021 tcggactggt aaaaatagtc gataacccct tgattcgaga taagtgaaaa gaaagatctc
     1081 cagtggtata aatactctgt gtatttttga aaaagatgaa agaatatata ctactaacta
     1141 taattaatcc actcaatatt actacttttt tatttatttt cattcttctt gtttatctcc
     1201 tttttagtca catatcgaga atctttcaac ttattaggtg caaatcgttt aaaacttgaa
     1261 tacaccacta aacaataagt cagaatactg atccataaag ccatgagagc agttggagta
     1321 tattttgttg taatttctat cttattttct ccagcattta catccacatt cactaagcca
     1381 tgttttttat aagagtcatt tacgacttta ccgtttactt ttacctgtgt acctaaataa
     1441 gcatatactg gtactgtcac tttttgcttt ttgttgcttg ccactgtgta agtaatgtta
     1501 ttatctgcag ctttatactt ccctttttcc cacgattctc ctacttttac ttcatgtttt
     1561 tctaagtgag gcctttcttt tatcgcttct gttggggcgt aatcgtataa tcctcccata
     1621 tctgatgttg tataacgtgg gagtgtttct tcattgataa caatatgacc gtgttttgat
     1681 tggataactt ttgcgtaatt ttcttttgct gctatagacg tgacgagtag aaacaccaca
     1741 gtagcgccaa tgattaacca tgaatatttt ttattcatag tctttttcat aaaaacaagc
     1801 gttatgatca ttgaagaaaa aaagatttgg atacctaaga tacgccaagg aaactggagt
     1861 aagcttatcg gtgttttttg aaacaaaaac catggaaata aattagttac gcatacccat
     1921 gtagaaacta cgccaacata gatgattttt tcttttttct ccatttcttt cagtatgaat
     1981 aaaggaaaaa tcaacgcaac caatcctaat aaccccatag tgtatgttgt aatatcgtta
     2041 tttaacgctt gaatgatact atctgaaata gaaagtgctt ctagctgcaa atctgttttg
     2101 aagggactgt gtatttcttg ataattcatc tgctgcaaca taggatacca aaaatagcta
     2161 gttaatgcac aagtcaaaaa aacagccttt actaaattca cgaccacaaa ttttatatcg
     2221 ttttttattt gcttgcttaa aaacaaacga actataatca tacaagcaat caatgcacta
     2281 cacaatagtg tagttaagac atgagtatac acaagtagac ttatactgac agataaagcg
     2341 atgacttttt tttctctatt gaatatcaat ccataagcat aatataaaac agctgggata
     2401 atcgtaagag cgattgcttc tgcaattgcg gatcgataat agatatcaac tgttcgataa
     2461 gttgcaaaag tatataaaca actaaataaa aaagcagcat ctatcttttt aaaaaacttt
     2521 tctccataat aaaaactaat cagaatcgta caaatattta ataaccaaat atatagaaca
     2581 taacttaata ttaagttatt tgaaattcca taaaaaacga ctgcaggaat tattgtaaga
     2641 aaaggataaa aatagttcag tccatctcca taattgttaa atgttgtata gttgattggc
     2701 ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat gaaaagataa atcacctgtt
     2761 gtataaattt cttgagcatt tctcataaaa tttactgaat ataaagtact aagtaagata
     2821 agaactacta aaagaaatgc ttttttactt attttcatag atttgttgtt ctcctttttt
     2881 aattctattt tcagtatact agaattgtct ttttttaata agttttgcgt taaatttttc
     2941 atagcactag tatgatatac tagaaaagaa tcaattttta ttacttttaa ataggagaaa
     3001 aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt ttatgttatt tctaagtttt
     3061 gcttacgtca tccctctatt catgtctaag ggaatctatc atagtgtcaa tcaggatact
     3121 tactttcatt tatctcgaat cataggacta gataatgtat ggagttctcc agtaaacttc
     3181 aataacttcg atcatcatgg aacaatgatg aatatttttt atccatggct tactctttat
     3241 ccagcttttt tattttataa aatgatggga aatcttgttt taagttataa tatctactat
     3301 ttttttatca cttttttaac aatggtcgtt tcttattttt ctatgaaaca aataaaaaac
     3361 aatcgttata tttcgctgtt attttcgatt atttatacat tttccgccta tcgtgccatt
     3421 gacatattcc atcgtgcttc attgggagaa gctgtcgctt taacattttt gcctttgata
     3481 ctaatggggt gttacgaaat ttatattaga gattaccaaa aatggtactg gttgtctatt
     3541 ggcatgacgc tagtagtgta tactcattta ctttctgtcg ctatggtttc tgtatttatt
     3601 ggaggaacgt tgtttctttc tttttatttt tgggaccaaa aaatcgctag actcttaagt
     3661 cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg gattcttaat tccttttatt
     3721 caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag tggtatggct
     3781 cctagtgata tgctgacaca tattttaaat aataactata acaactatac tattggatta
     3841 ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac tgctgatgat
     3901 ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt atttccttgg
     3961 caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt aaatggtttt
     4021 tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt ttctactaaa
     4081 aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca tctctcagga
     4141 gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc tgaagacgct
     4201 gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc aatctatcat
     4261 cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa tccaacaact
     4321 cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa aaatactgtc
     4381 ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg caaactagta
     4441 caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg tataaataaa
     4501 gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt atctatcgtt
     4561 actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca accgagaaga
     4621 gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg tacacataaa
     4681 caggctaaaa agtctttttt tatatttctt taatttgctt catacattat ttaacatcga
     4741 tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc gataataaac
     4801 ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct
     4861 ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt
     4921 ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag
     4981 catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga
     5041 ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc
     5101 attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc
     5161 tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct
     5221 tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta
     5281 acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc
     5341 tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc
     5401 ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc
     5461 tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt
     5521 tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt
     5581 gtagcacaaa atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga
     5641 aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa
     5701 tttgacggct caagttttac tgtttgagat gcaagatact caacctggca atgggcacat
     5761 aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat
     5821 ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca
     5881 gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa
     5941 gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg
     6001 tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat
     6061 cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac
     6121 taacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag
     6181 accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc
     6241 tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac
     6301 tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc
     6361 tccaataaca attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac
     6421 agaaacctct atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa
     6481 atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat
     6541 tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact
     6601 tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc
     6661 gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa
     6721 taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca
     6781 taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag cagtcaacgt
     6841 catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga
     6901 tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat
     6961 atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa
     7021 aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa
     7081 tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc
     7141 agcgattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa
     7201 tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt
     7261 tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataagacgcc
     7321 aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt
     7381 agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat
     7441 aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca atgttcccac
     7501 taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat
     7561 tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa
     7621 caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa acaatattcc
     7681 gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa
     7741 atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg
     7801 tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt ttgtactttt
     7861 tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat gggcaactta
     7921 tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta ttcttgaaaa
     7981 aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata atgatggttt
     8041 tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac cctggtcgat
     8101 tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg atcccacttt
     8161 acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt
     8221 ttgcctttat attcctaata gccacactag tttgcaatat ctttttttac gtacaagctg
     8281 gcagcttgaa tattcgtgat tattggctta tcctaacacc tatcagtcag aattacttta
     8341 cttatgctgt ttcttgtact ttgctttttt gtagtattcc ttatacagtt ggatttttaa
     8401 ataaattatc gaaaacaaca attaaatatc tactattatt tttaacaatt ggattagttg
     8461 gctgctctac tctatttaat aaagacccgt ttgcattaca gaatggacag agtattttat
     8521 ggatttttta tttattttta atgggttatg gtttgaaaga atggaactgg aaagaaaagg
     8581 tccgctataa gcttcctcaa gtatttatca gcttgattgt tttattcggt ttgattattt
     8641 tgatgacaca aatttcactc atcatcagag gaaatacaga tacagcgctg aggttttcta
     8701 aaccatattc tctcttcgca atgtattata ctttctcttc gtttttattt ttagaaatgc
     8761 tatctgataa attaggtata aaaggaagag gaaattttct tgctgtattt ttgattgtca
     8821 ctcaagttat atgtaatttc cctttagttt cttatcgcgt atctacattt atgaaacgtg
     8881 aatttccaaa ctcagggaag gcatggctga taaatatcgg gcagtttttt atttgatatt
     8941 tattagcagt gtccctccta gttatctttt tattttatat acaaaaaatc cgtttcattc
     9001 atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag cgttggaaaa
     9061 aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca tttttttact
     9121 gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa gaaactatcc
     9181 aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca atcatcatta
     9241 ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac gccttgactg
     9301 ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg
     9361 aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa ctattatcga
     9421 tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta ttgattacta
     9481 gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat tggaaacgtc
     9541 gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc attaatcaca
     9601 aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact ttctttaacc
     9661 agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat atcgacatta
     9721 aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata atgaaaaagt
     9781 acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac aatcagacga
     9841 tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat ctgacagtac
     9901 ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga ggattgatgt
     9961 tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg acaggcttga
    10021 gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg gagcgtcaga
    10081 aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat ccttttacag
    10141 ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg ttctattatt
    10201 tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca cgcatatcgg
    10261 atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa acgtcacaat
    10321 tcatccaatt atccactatg caaaaccata tgccttatga tgattactat agtgaattga
    10381 actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta caaacttata
    10441 tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa atcgacaaaa
    10501 tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc tatagtggga
    10561 atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt tataacaaca
    10621 agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct aataactttg
    10681 gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg ttgttgacag
    10741 aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg tctaatcgtt
    10801 ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa aaaaatttaa
    10861 ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat cttgttgcag
    10921 gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa ata
//
LOCUS       DAJSBM010000076        10941 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJSBM010000076 DAJSBM010000000
VERSION     DAJSBM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.077"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(13..204)
                     /locus_tag="ONT67_002365"
     CDS             complement(13..204)
                     /locus_tag="ONT67_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356084.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(345..665)
                     /locus_tag="ONT67_002366"
     CDS             complement(345..665)
                     /locus_tag="ONT67_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356085.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(688..1122)
                     /locus_tag="ONT67_002367"
     CDS             complement(688..1122)
                     /locus_tag="ONT67_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR2356086.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            1457..2095
                     /locus_tag="ONT67_002368"
     CDS             1457..2095
                     /locus_tag="ONT67_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCR2356087.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(2419..2502)
                     /locus_tag="ONT67_002369"
     tRNA            complement(2419..2502)
                     /locus_tag="ONT67_002369"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Leu,seq:cag)
     gene            3030..3530
                     /locus_tag="ONT67_002370"
     CDS             3030..3530
                     /locus_tag="ONT67_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356088.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(3720..4064)
                     /locus_tag="ONT67_002371"
     CDS             complement(3720..4064)
                     /locus_tag="ONT67_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCR2356089.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(4218..5138)
                     /locus_tag="ONT67_002372"
     CDS             complement(4218..5138)
                     /locus_tag="ONT67_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2356090.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            5325..5816
                     /locus_tag="ONT67_002373"
     CDS             5325..5816
                     /locus_tag="ONT67_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCR2356091.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(5840..8215)
                     /locus_tag="ONT67_002374"
     CDS             complement(5840..8215)
                     /locus_tag="ONT67_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR2356092.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            8541..9167
                     /locus_tag="ONT67_002375"
     CDS             8541..9167
                     /locus_tag="ONT67_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCR2356093.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            9190..9894
                     /locus_tag="ONT67_002376"
     CDS             9190..9894
                     /locus_tag="ONT67_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2356094.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            10086..10919
                     /locus_tag="ONT67_002377"
     CDS             10086..10919
                     /locus_tag="ONT67_002377"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR2356095.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         3254 a         2144 c         1899 g         3644 t
ORIGIN      
        1 taatcgtttt ttctattctg ttataccaaa aaaatgtttg aatcggtgaa ggatcgttcc
       61 ttgttttaat tctataaatt cttgtgcgat cgggtacttt tcttccaaat tttctttgtt
      121 cttttcgatc aggcttttcg cttcttcata ttttttatct gcacatagtg cttttacttc
      181 ctgtaatact tttcctgctt ccatttgcct caccctcttg ccattttatt gctttaagtt
      241 ttatttaagt tatacgcatt attctagttt catcccaaat aacctttttt catttttact
      301 cctcgaacta atcagttttc taaaggctga ttggttcgtc ttttttattt ttcctccatt
      361 tcattctgct cttctgttaa tttatctaat gctttgattg atgctgcggc tcttgcataa
      421 tcatagctgt ataattccat aaatttatca ggcagaacaa accacgctgg atgataaccg
      481 ttagctgtga cagggaatcc tttatcttgt tggttatctc catcccatct cagagcaaca
      541 cctttttttc ctttccactt tactaaagcg atcgagtatc ccttctttgt ttgtgtgccg
      601 tcttcaataa ttgctaaaat ttctattttt tcttttggcg cacggacttc ttgaggacga
      661 accattttct attcctccct tatctcatta cttcacgacc aacacactgc aaggggcatg
      721 tgatacgaca taagaagcgg tcgatccaac taatgcttgt tgtatagcac cttttcctgt
      781 cgctcccata acgatcaaat caatattttc ttgtccaggg atgacattgg caatcaaatt
      841 tcttggatct ccagtttcaa ctattgcgtg gatctcagca atgccgtact ctttggcatc
      901 attgattttt ttcagcatgg ctacttctgt tctttgtttt tcatctgcat agatttttga
      961 aaaggcatag gcactatggg tcagctcaac tttgttgatg attgacacga taaataagtg
     1021 ggcttcattg tcctttgcta agtcaagggc ttcagcgaac gctttgatcg attgtcttga
     1081 tccgtcaact gctaccatga tgttctggta tcttttactc attggaaccg cctccttttt
     1141 cttttattgt aacatctcaa gcaaaaatac aaaaaatata cgcaatatgt aaataaaatg
     1201 atgaacaaaa gcaatttctt tcaagttatt tatacatttc ttttctcatc agaacacctg
     1261 ttctaatccc attatttctg cattatgtga aattcctcat catttttcac atcgagcgaa
     1321 catcattctg ttacaaaaaa cgtggatttg tcataaattg tcatgcttct tttacctatg
     1381 aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac tttttaacga
     1441 ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa ctttggctgc
     1501 cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata ccgttcaatc
     1561 aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct tgatcaatgc
     1621 cattgcagaa tcaaattcaa tttctgatat taacctgatc tactcaggac aacaattaac
     1681 aatcccaaca gaaggctctg cacaagcagc tgctgaacct caagcagccg ttcaagaagc
     1741 accagtccaa gctgaacctg ttcaagctga acaacctgtt gtacaagaaa cggttcaaac
     1801 ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct cctgctgtaa cagaaactgc
     1861 tgctactcca gcaagtacaa gctctgcaaa agaatggatc gcacaaaaag aatcaagcgg
     1921 ttcttacaca gcaacgaacg gacgttacat cggccgttac caattagatt cttcttactt
     1981 aaatggtgac tactctgctg caaaccaaga aagagtagca gaacaatatg ttacttcacg
     2041 ttatggttct tgggaagcgg cgaaagcatt ctgggaagca aacggttggt actaagattc
     2101 attatttgaa cgtttaaaca cattcaaaat atagtaagag gtcatgcgat ttattctttc
     2161 gcatgacctc tttttgagtt taagtttagt ctttattcct taactatcgt ttgccagtat
     2221 ttcttgtatc agaatctata aatctactat ttgaatcaat ctattaggtt ttaaactgtc
     2281 ccaccttcac acaaattttt tttgatttat ttgaatctgg ttccgttgtt taaaattttt
     2341 cctatattct tttagataat atgttatttt ctttctcatc gctccaggtg taccttctct
     2401 ttataaaaaa acaaattatg cggctaagaa gactcgaact tccacgaggt caccctcact
     2461 agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc gcatcacatc ttttagttta
     2521 ggattacatg tgttttttgt caagaacata tttttgttct ttttcatttc tgtacgccct
     2581 cccttatgcc atctacacaa ataattcatc cgagcaactc catcatctat tgagggttgg
     2641 tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt aaacgaacga ctctgggatt
     2701 agtaactatt ttattttttt cttcatttct tttttcctct ttttctcgat tcagctatct
     2761 tctttcattt ggatattgat tatttcttct gttgattact atccttctgg aattctagac
     2821 gctgaagtaa ttcacgtttt ctttcttcta gagatcataa gtaacgaatg aacgaacttc
     2881 aaataatttt tttattgtaa ttcatatttg tattgttcga tgattcttaa tacgatatga
     2941 cacaataaat aaaaataggc atggctattt acaacaatag tgctatagta gaaaacaaat
     3001 gaataaggtt acttaaagag gtgaatacga tgaaactgat caaactatgg gaagcagatt
     3061 taaagaaagc ttatcagcta caaactagct ttggaccaaa tgagagtgga tttatcaaca
     3121 atgcttatgg ctatacattt gctgaatttt tagattatgt aaaaaaatgc gaaaaaacgt
     3181 ctttaggaat cgatcttcct gagagttacg ttcccgcaac cgtctttatt ttagaaagtg
     3241 atgaacatga gtatgtagga atttttaatt tgcgccataa actgaacgaa acattaagaa
     3301 acggcgctgg ccatattggt tacggcatct cccctaatta tcgtaaaaaa ggatatgcca
     3361 cacaaggatt agcattagta ctacaagagg caaaaaaaat cggcattgat gaggcacttt
     3421 tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca acaaaaaaat ggggctgcga
     3481 tttatcgtga agatgagaaa gaatactata cgaaaatcaa actgatgtaa aaaagaagga
     3541 ttccaaaagg aaagggattt gaatgtcctt tatcaataga acacatttat ttaattaact
     3601 tataatatag cagacaaaat tttataaaca aagaaacctt ttatctcttt tcttctagaa
     3661 aggataaaag gcttttttgt ttttcctatt tgcttctcat agctagtcag ccaagatttc
     3721 tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct tatctgtcgt ccatgctgga
     3781 taccagacta gtttgacttc tgctttcgtt acttctggca cttcttttaa tgcgtcatgg
     3841 atagattctg ttaaaatatc tgctaatgga cagcccatag ttgttagagt catcttaatg
     3901 actgtttctc cattttcagg attgaattct atatcataaa tcaagcctaa attgacgatg
     3961 tcgattccta attcaggatc aatcaccatt tccaaggcag caagaatacg ttctttgatt
     4021 agttctattt cttgtccaga ccgttcttgg tttgcttcgc tcatcgtacg acactcctta
     4081 acacgatttt tatttatcat actctttttt tagctgcttg taaatgagaa tttagaagta
     4141 agattctcgt ttaaatttat aaaaatgttc ttattagatg taatagatta aatttattct
     4201 aaaataaacc tgattcctca ttttttttgt gaacgatttt ttatttctgg caatacgcca
     4261 gttgttcgat acattttttg ttcgttttca tttgcaaacc ggccaaatac tgaatgagtt
     4321 ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa cgatctgatc aagatcatac
     4381 atttcgtagc gggtgatgac catcatgatc atgttccctt gtttttttga atagccaccg
     4441 acagacggca gtaatgtcat tccacgtacc atccgttttg ctagttcttc tgttacctct
     4501 tctggcttga cggtaacgat cattgctgtt actttttgat gactcgtgtg gatggcatca
     4561 attacttgag tcattgcata aatggaaata atcgtataca aggcactttc ccagttaaag
     4621 aagaagcctg cgatgactac aatgatacca tttagaatca gcatgtagtt tccgactgtt
     4681 tttccagttg tttgtgaaag tacaagagag atgatatcca tcccaccagt tgtaaagccc
     4741 atttttaaag ataatcctac gccaagacct aataaaacac cgcctactag ggcattcatc
     4801 aaaatattat cagtgatctg tccgactgga agcagcatcg tcatcaccga tacacataca
     4861 acattcacaa aactaaggat cgttgctcgt tttcctactt tgagaaaccc taatacaaac
     4921 acagggatat tcaaaagaaa aataaatgtt cctgtgtcta tcttgatatg aaaccattca
     4981 tataaaagcg aggaaatgat ttgagcgatc ccattcatcc ctgcagaaaa gacattggct
     5041 ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc cggttatcaa aataaccact
     5101 attttcttta atctttcatt tttatgatac gcagtcataa ttttactcac aagcacctct
     5161 cccctacttt ggtattccaa atgtaagaat aacagtttat ctattaaaat aaaatacttc
     5221 gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc tgccctaatc ctatgataag
     5281 atagatgtat ggtaaatatt tatatgtata tcaggagggt tcctatggca aaagatgcta
     5341 gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa aaatgcgatt cagcaaacaa
     5401 caaaagaatt gtccaatcgc tttgacttca aggattctac agtagaaatc aaattagaga
     5461 acagcaatct agttgtattg agcgatgatg atttcaaaat cgaacagatc aaagatgtat
     5521 tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa tatccatttt tccgaatcag
     5581 aacacgctct aggtggcaaa gctcgacaaa ctgccgattt ggtcaacgga atcgatcgtg
     5641 aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa actgaaagta aaagcacaaa
     5701 tccaagaaga tcagattaga gtgacaggta agaaccgtga tgatctacaa gctgtgattg
     5761 ctttattacg taaacttgac ttaccaatcg agttgcaatt cactaactat cgttaatcaa
     5821 aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt tactgcttct tttgataacc
     5881 aaaaacgctg tattccttct tccaattccc caacacttcg tacttctctt gctgtttgcc
     5941 aatgttgtgg gaataaacgt cgataagaaa aactagaaaa tcgttgatct gccaatacaa
     6001 cgattccttg atccgccatg ccacgaatca ctcgaccacc agcttgcaag actttgttca
     6061 ttccaggaag ctgataagcg taagcaaatc caaggtgttc cttctcatca taatatgatt
     6121 tgatcagttc ctgctcatga ttcatctgcg gcaaacctac acctacgatc atactgccga
     6181 tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat cccgccaagt acgcaaaatc
     6241 caatcaatgt ttcagcggga tttgtttgaa attttgttaa aaatcgttcg cgttcttcct
     6301 cattcatgtc cgtttcttgg attagcacat tgctgtcggg atattgctgt ctgaatgctt
     6361 ccaccgcaag atctaaatat tgataagaag gaaagaaaac gaaataattc ccgacctttc
     6421 catcagcaaa agcatgaatc gtctctacta attttgccag actgtgttcc ctttttcgat
     6481 aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt tccttctggg aaaggactcg
     6541 gcagtttgta agccagactt ttttgtcccc ccagtgtttc ctgataataa gagagtggtg
     6601 aaaaactagc agaaaaaaga atactgcttt ttcctttatc caacgactgc tcaagaaaca
     6661 agcttggatc aatacaaaac tcttttacta tcagatcgcg atacgttttt tcgactgttg
     6721 tttcgtagtg atcatcataa aattcactga ccttcaaaaa atgaagcaaa tcaaaataat
     6781 aaggaagcaa ttgttcttgt gatgggtgct caggaaattc tgccagccat tctttaattt
     6841 tttcgcttaa ttgataacct gcctttacta aagattcagc cggtgctttt tgatgatgat
     6901 aatcccaatg atcttctttt gctatttctc ggatcgaatc aaattctttt aatagtttgt
     6961 tgaaacggcg atgaagtttt ttgaattcct ttggtatagc ttccttactt cgtttcgttt
     7021 tttcataaga tagttctgcc gaatacatct ctcgggaacg attcaccaaa ttatgtgctt
     7081 cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc aaaaaaacgt cttaaataaa
     7141 cagttggatc aaataaataa ttgtaatccc caatgattac gtcacaccag atactgacat
     7201 ctaatgatag ttcgaaagga caaagtgtat gctttcttgc ataagtttcg atgacttcac
     7261 gagtgatctg attctcatga ttcaaaagat cccataaacc ttcattaatc cgattgtaat
     7321 aaccattggc atatgggcac tgatctggat tacaggtcgt ctcatctaaa aagcagatct
     7381 tgtctttcgc cgtcaaagtg acacttttta cttcagctcc tgtatctttt aatgctgtca
     7441 tagcatcttc agctacttgt ctagttattg ttttagccgt tagataaaag atcttttctc
     7501 cctcttcttc ccctacagct ttcaaagcgg ggaataacgt agagattgtt tttcctgttc
     7561 cggtaggtgc ttctacaaac aatttctgtt tcgtacggat cgttttataa gcagctaccg
     7621 ctagttcacg ctgacctttc ctatactcat catgggggaa tttcaaagcc atcaaagagg
     7681 cattgcgtac agtgcgccag ttttcttgaa aaacaagcca ttctttataa tcttcagtca
     7741 gctttttaaa aaaagtttct aactgctctc ttgtctgatg ttcaaccttt ctcgtgatca
     7801 cttcttctgc cgtctggaag taagtcaatt gcagatttat ttcttgtaaa tcatgttctt
     7861 gactatagat ataggcatac acccttgctt gatgaaagaa aagttccact tgttctggtt
     7921 ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc tatataataa ataccgtctc
     7981 ttttgaaaat accgtctgca cggccttcaa cgatcaggta atattcatct aattgaactg
     8041 ttgtttttaa aaagacttct ttttcatagt tttctcccgc atctttttgg agcttgcggt
     8101 ggattctcgc tccttctaat gcagtatggt tacttttttt acgattgtcg atacttccac
     8161 gtcgcaaaat aaattctact aagtgccgca cagatatttt tgactgtttg atcatttcca
     8221 ccacctcttt ctctctaaga ttacctgaaa aaacctagtt tagcaaaaag agctctcttt
     8281 ccaaacataa aaaaacaccc tgctgattag gcagggatat aggatataga atttggattt
     8341 ttggatgaat ctaatgtaac aaggacatat taaaatgcta tgaaaactta ctttccttct
     8401 ctttagaata tccaaatact tttcatatag tctgataatt cagataagta aagagaaata
     8461 agtacctgtt tttgtttcct agcaatgaat gcgttatact aatggatgaa agaagttttg
     8521 gctatcaagg aggaatgcac atgcatatcg aaagattaaa aacaggaatg attgaagaaa
     8581 actgttattt ggtctacaac gaagaagcac ttttgatcat tgaccctgga gaagatgcgg
     8641 aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc ggtagcgatt ttgcttaccc
     8701 atactcacta cgatcatatc ggtgcagtgg aacctttacg cgcgtattat caaatcccag
     8761 tttatgtcag cccactagag caaaaatggc taggtgatcc gattttgaat ctttctggac
     8821 tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc tgcagaatat gaatttgaaa
     8881 tgaaaactta tactttagga aacatgtctt ttcgcgtcgt ccctacccca gggcattcta
     8941 taggaagcgt cagctttatt tttgatgatt ttgtcgtttc tggcgacgcc ctgtttagag
     9001 gaagtatcgg tagaaccgat ctgtatacag ggaatttaga acaattactt catagtattc
     9061 ggacccagct gtttgtatta cctgatgaat ttgcagtcta tccaggacat ggcgatgcaa
     9121 cgacgattga acaggaaaaa aggacaaatc cttttttcaa tcgttaaaac gacttaggag
     9181 gagaataaaa tgacagaaac tacactttat atcgtacgac acggaaaaac gatgttcaat
     9241 acaattgaac gagtacaagg ctggtgtgat acacccctca caaagcaagg acaagaaggc
     9301 attcattatt taggtcgcgg gttaaaaaat gtcgactttt catttgctta ttctagtgac
     9361 agtggtcgtg caatcgaaac tgcccgtatt attctaggag aacattctaa gggaaaagaa
     9421 attccttatt ttattgaaaa aaggattaga gaatggtgtt tcgggtctct tgaaggtgga
     9481 tatgacatgg aaatgtgggg tgtgatccct cgtgtattga attttaaaac atatgatgaa
     9541 atgtttacca cagaagtttc ttttgaacaa attgctaatg ctgtatacaa cttagatacc
     9601 gctgggtggg cggaaactta tgaaacattg cgagaccgtg tatggacagg atttgaagat
     9661 atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt tagtcgtatc tcatggtttg
     9721 acgatcgcct ttttgcttaa tctgatcgat cctaaacagc ctgttcgtgc tgggctttta
     9781 aatggcagcg taacaaaggt cacttatgca aacggtcgat tttctatcca cggcatcaat
     9841 gatacgtctt atatcgataa cgggaaaaca catagtcgcg actcgtttct ataatgggga
     9901 tattttaatt tttaccagag aatcataaaa ggcaaagtct gaacgagggc tttgtcttct
     9961 tttttctctt cgtgtactta tctgctttat ttctcaagtt tttcttaata ttaaaaaaca
    10021 gattgggttt ctcaaaaaat aacggtatac tataagatgt atcttaattg ggaaggaagg
    10081 aatttatgtt ttttgcaaat attatcaaag cagtgattct cggtattatt gaaggaataa
    10141 cggaatggct gcccatcagt agtactggtc atttgatcct agcagatgaa ttcatcaaac
    10201 tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt tatccaatta ggggcgatct
    10261 tggctgtcgt cgttctttat tttcacaagt taaacccatt ttctccaagt aaatcaacgg
    10321 tagaaaaaaa agatacgtgg acactttggt ttaaagtgat cgttgcttgc ataccagcca
    10381 tcatcattgg tttgcctttg gatgattggt tagaagcaaa tttccacaag ttcttaccag
    10441 ttgccattgt tttgatcgtt tacggtattg cctttattat tgtcgaaaaa cgcaacaaaa
    10501 acaaaacacc tcgttggact tctctgaatg actttacgta ccaagcagct cttcttgttg
    10561 ggttattcca agtcttggca cttgttcccg gaacatcccg ttctggcgca acgatcctag
    10621 gatctatctt gattggtgct tcgcgttttg tcgctacaga gttttcattt ttcttaggga
    10681 ttcctgtcat gtttggcgca agcggcttga agatcgtcaa atatttagct gaagggaatt
    10741 cattcaaaat ggaagaaact gttgttcttc ttgtaggaac attcgtatct tttgtggttt
    10801 ccgtcttagc tatcaaattc ttactaagct atttgaagaa aaacgacttt accgcttttg
    10861 gctggtatcg tattatcctt ggtatcattt tgattggtta ttggtttatt gcaatgtaga
    10921 aaaaaccaga acacttctct t
//
LOCUS       DAJSBM010000077        10929 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJSBM010000077 DAJSBM010000000
VERSION     DAJSBM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.078"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..287)
                     /locus_tag="ONT67_002378"
     CDS             complement(<1..287)
                     /locus_tag="ONT67_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356096.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="ONT67_002379"
     CDS             429..1547
                     /locus_tag="ONT67_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR2356097.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="ONT67_002380"
     CDS             1675..3051
                     /locus_tag="ONT67_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR2356098.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="ONT67_002381"
     CDS             3452..5032
                     /locus_tag="ONT67_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR2356099.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="ONT67_002382"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="ONT67_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="ONT67_002383"
     CDS             complement(5658..5978)
                     /locus_tag="ONT67_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCR2356100.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="ONT67_002384"
     CDS             complement(6126..8360)
                     /locus_tag="ONT67_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356101.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="ONT67_002385"
     CDS             8839..9105
                     /locus_tag="ONT67_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCR2356102.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT67_002386"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="ONT67_002386"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR2356103.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3323 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaat
//
LOCUS       DAJSBM010000078        10817 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJSBM010000078 DAJSBM010000000
VERSION     DAJSBM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.079"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT67_002387"
     CDS             complement(25..162)
                     /locus_tag="ONT67_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2356104.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT67_002388"
     CDS             complement(159..1343)
                     /locus_tag="ONT67_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356105.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="ONT67_002389"
     CDS             complement(1599..1853)
                     /locus_tag="ONT67_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356106.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="ONT67_002390"
     CDS             complement(1878..3020)
                     /locus_tag="ONT67_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HCR2356107.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="ONT67_002391"
     CDS             complement(3080..4432)
                     /locus_tag="ONT67_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCR2356108.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="ONT67_002392"
     CDS             complement(4503..4847)
                     /locus_tag="ONT67_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR2356109.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="ONT67_002393"
     CDS             complement(4857..5231)
                     /locus_tag="ONT67_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2356110.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="ONT67_002394"
     CDS             complement(5244..5558)
                     /locus_tag="ONT67_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCR2356111.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT67_002395"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="ONT67_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="ONT67_002396"
     CDS             complement(8976..9638)
                     /locus_tag="ONT67_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356112.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="ONT67_002397"
     CDS             10255..10485
                     /locus_tag="ONT67_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356113.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="ONT67_002398"
     CDS             10491..10790
                     /locus_tag="ONT67_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356114.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DAJSBM010000079        10497 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJSBM010000079 DAJSBM010000000
VERSION     DAJSBM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.080"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            23..646
                     /locus_tag="ONT67_002399"
     CDS             23..646
                     /locus_tag="ONT67_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356115.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="ONT67_002400"
     CDS             1096..2145
                     /locus_tag="ONT67_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2356116.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ONT67_002401"
     CDS             2142..3023
                     /locus_tag="ONT67_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2356117.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ONT67_002402"
     CDS             3026..3796
                     /locus_tag="ONT67_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR2356118.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ONT67_002403"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ONT67_002403"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR2356119.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ONT67_002404"
     CDS             5107..6270
                     /locus_tag="ONT67_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2356120.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ONT67_002405"
     CDS             6387..6797
                     /locus_tag="ONT67_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR2356121.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ONT67_002406"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ONT67_002406"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR2356122.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ONT67_002407"
     CDS             9370..9654
                     /locus_tag="ONT67_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356123.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ONT67_002408"
     CDS             9814..10050
                     /locus_tag="ONT67_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2356124.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ONT67_002409"
     CDS             10047..10445
                     /locus_tag="ONT67_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCR2356125.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3657 a         1766 c         2226 g         2848 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatat
//
LOCUS       DAJSBM010000080        10086 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJSBM010000080 DAJSBM010000000
VERSION     DAJSBM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.081"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(247..759)
                     /locus_tag="ONT67_002410"
     CDS             complement(247..759)
                     /locus_tag="ONT67_002410"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR2356126.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT67_002411"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="ONT67_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR2356127.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="ONT67_002412"
     CDS             complement(3212..3655)
                     /locus_tag="ONT67_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCR2356128.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="ONT67_002413"
     CDS             complement(3656..4444)
                     /locus_tag="ONT67_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2356129.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="ONT67_002414"
     CDS             complement(4441..5382)
                     /locus_tag="ONT67_002414"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR2356130.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="ONT67_002415"
     CDS             complement(5559..6146)
                     /locus_tag="ONT67_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCR2356131.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT67_002416"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="ONT67_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCR2356132.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="ONT67_002417"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="ONT67_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR2356133.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="ONT67_002418"
     CDS             complement(9063..9998)
                     /locus_tag="ONT67_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR2356134.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DAJSBM010000081        10008 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJSBM010000081 DAJSBM010000000
VERSION     DAJSBM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.082"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(472..696)
                     /locus_tag="ONT67_002419"
     CDS             complement(472..696)
                     /locus_tag="ONT67_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356135.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            887..3664
                     /locus_tag="ONT67_002420"
     CDS             887..3664
                     /locus_tag="ONT67_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2356136.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3745..4164
                     /locus_tag="ONT67_002421"
     CDS             3745..4164
                     /locus_tag="ONT67_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCR2356137.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4176..4646
                     /locus_tag="ONT67_002422"
     CDS             4176..4646
                     /locus_tag="ONT67_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR2356138.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4663..5442
                     /locus_tag="ONT67_002423"
     CDS             4663..5442
                     /locus_tag="ONT67_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCR2356139.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5429..6250
                     /locus_tag="ONT67_002424"
     CDS             5429..6250
                     /locus_tag="ONT67_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR2356140.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6267..7316
                     /locus_tag="ONT67_002425"
     CDS             6267..7316
                     /locus_tag="ONT67_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2356141.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7329..8333
                     /locus_tag="ONT67_002426"
     CDS             7329..8333
                     /locus_tag="ONT67_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2356142.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8966..9985
                     /locus_tag="ONT67_002427"
     CDS             8966..9985
                     /locus_tag="ONT67_002427"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR2356143.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1649 c         2092 g         2907 t
ORIGIN      
        1 aatctttttt gctacgtaat ttttgtaaaa aagtgggttg agagaaatct aattgatttt
       61 caaagaagga gtatgaaaat gagaaaaata tttattcgtt tattgtgggt actatcttag
      121 aggaataaat aatagtggag aaaactatgg tttggcgcag attctatcag gtttgatgga
      181 acgaagacaa cctcccgaca tcaagtcaaa agctacttga gattttgatc tgaaaataaa
      241 aaaagaaaac aaggtaggaa catcaatgtt tctaccttgt tttcgtaatc aatgggaaaa
      301 aactaagagt ggtgtacgta atcaataaaa aggatggtga caacagtacc tctttactct
      361 ctgaaagcat cataactcgt ttttcctcat tatgccaata aattgttgtt ataccacata
      421 taaggatttg taatagctaa aaacagacag gaaagttgaa atctgtgaat cttattttgt
      481 atggaaggat ttatagaacg caaaaatcaa gataaagatg aataaaaatc ctgtttcggc
      541 tatatattgc gtatctacgc caataaaacg agtttgttct gtcatagcca tataaagcaa
      601 aggcaaaaat acaactacag cgagataaga tccgatgatt tctcgaaaag ttttccagga
      661 agtatgtttc ctgtttttat atttatccga tttcattgaa tagtcatagc ctccttattc
      721 tttcatatgt tgaaattcca gtataaaaac tggaaggaac tgtacgtttt ttagaataaa
      781 aattataagg gataagtagg ctgctgtcta taataagata tatttttcaa ttctttatta
      841 aagattttat tgacaaaatg aatccgtttt ctatgatgga ggaggggtga ataagttgtt
      901 gaagaaggaa attttatgtt acttagagaa ccagacagcc tttttcgatt tagaacatat
      961 gaacgaggtc tttacagcac aaaggttagc tgatcatttt ggggtaaaaa gaaacacagt
     1021 cagtcattat ttgaaccagt taaatgatga gcaactacta gtcaaaatca attctcgacc
     1081 agtgatttat ttccacaaag aagcttttga aaaacaattt tttaagttac gcacgaattt
     1141 ttatttttct atcgatgatc tcaaagagga acagcctttt ttcgcgcaac caaaagactt
     1201 attctctttt atgatcggat acgacgccag tttgaaagaa agtatcgaac aaatcaaaac
     1261 agctctttac tatccagatg gcggattacc acttttgatt acaggcgaaa gtggcacagg
     1321 taaaagttat ttagttaatt tggtctatca gtattgtctg cttcatgatc tgctagaaga
     1381 ttctgcacca ttcatcactt ttaattgtgc acaatatgca gataatcctg aacttttgac
     1441 tagcaatttg tttggacatg ttaagggagc gtacactggt gcagaagaaa ataaaaaagg
     1501 ggcttttgaa gcagcagatg gtgggatctt gtttttagac gaggtacatc gcttaacacc
     1561 agaaggtcag gaaaaactgt tcacctatct tgatcaaggt gtgatttatc gtatgggaga
     1621 acctaatttg tcacgccgga tcaaaacacg tctctttttt gctacgactg aagagattac
     1681 tagtcatttc ttaacaactt tcattcgaag aatacctatc cgaatcgaac tacctgcact
     1741 taatcagcgt agtcgtaatg aacggctaga actgatttac tctttttttc tcgaagaaca
     1801 aagaaaaatg gggagccctt tgtcagtcag tggaccagct ttgtcacttt taacaagtca
     1861 gaaattatcc ggaaacatag gagagctgaa aaatatcgtc aaagtttcag cagcaaaagc
     1921 atatgctgaa cagcgtgaac agcctgaaat ccatgtcacg atccatcatt tgcaaaaaga
     1981 actacttgcg cagcctatta ctaaaaatac agcacagcaa ggaatctata ttacgcaaga
     2041 ccagacctta gagcaattaa tggagaagca acagcccgaa cagcagcgaa tcgttaaaag
     2101 ttttgagcaa atcctaattg atttcaagaa aaataactgt ctgctatatt cttgcgaagg
     2161 aaaattaaag caagaagtga ttcaattatt tgattttttg ctgtttgaga ccagtagaca
     2221 agaaaaacac gagctgctgg tttattggac acaatctatc cgagagacct ttcgacaaat
     2281 ggaaactgcc tatcaaatca aattcgatgg aaacagtgtc tatgccttga gttactatct
     2341 ctatcagcga agcactatca aatggttacc ggaagaacat gatattatcc aactaatcaa
     2401 agaattagaa caacaagttt ctcagtctta tccatctagt tatcactatg tccaacgaat
     2461 tctggaatta agtaagatca aattagatat tgaagacaca agtatggatc ggattttact
     2521 gacgatctat ctaaaaaaag cgaaatggtc aaaagaaaaa cgactgccaa aagcgatgat
     2581 tgctgctcac ggttatgcaa ctgctagcag tatggcaaat gtagtaaatc gtttattaca
     2641 agaggatcta tttgaatcat ttgatatgcc actagatgtc acaccacagc agattgcttc
     2701 agaaatcctt gattattgcg aaaacagcga tgtttcaaat ggattgatca tattagtaga
     2761 catgggatct ctgaaagaaa tccatcagtt ttttaaaaaa cagttatccg taccgctttt
     2821 gattttgaat aatgtgacga cacctttagc aatcacagta ggggaatgcc tgcaaaaaaa
     2881 tgtatcatta gaagagatag cagaagagac tgttcgacag atccagccag aatggcgact
     2941 gctctatcca gaggaaaaca aacccaaagc actcattact acttgcttca ctggaatcgg
     3001 tacggcggtc cacttaagtg aactgctaga aaaaagctta cctacggctt gtcagttgaa
     3061 aatcatccca tatgaatatc agcaattaaa agataagaaa aacagtgacc cattattttc
     3121 catatacgaa gtcgtaggaa tgattggcac cacagatcct gatatcacca atatcgacta
     3181 tctttcatta gaagacctca tctcggggga aaagatgtct gtactagctg aatggttgga
     3241 cactactatg aatgcttcag agaaggaaat attcaatcag cgcatcattc gtaatttttc
     3301 attggaaaaa gtactggatt ccgtgactat tctagataca gagaaggtga tgggagaaat
     3361 cgatcatttt atgagagaat tagaggtgca gttgaatcga agcatcagca atcccaaaaa
     3421 attagcgttg tatgtccatg tgagctgtct cattgaacga ttgatccgac aaatgccaat
     3481 cgaaacctat caaggattag agaaatggaa gcagtgtcaa aaagaagggt tatctgctat
     3541 taaatctgcg tttagtgtca ttgaagagca ttatagtgtc atgatcccca attctgagat
     3601 tgcctatatc tatgatattt tgagtgaaga tacagagttt ctctcgatag aagaagaatt
     3661 ttgaacataa gcaagttgtt gccactaatt ttggcatgca acttgctttt ttagtatgtg
     3721 taagtgaaaa gaggagatga aaaagtggat agagaaatta tcttagcttc ccatggaaaa
     3781 tttgcttcag ggatattaga ttcattagag ctgatctatg ggaaaaatca ttctattact
     3841 gtattcgatt gctatactga tgagaatttt gatttagcgg aaacagtaag gcagcttttt
     3901 atgaggtaca aagataaaga aatcattgtt ctgacggatc tttttggcgg aagtgtcaac
     3961 aacgaattct tacaatatat caatcgggat catgtctatc tcgttgccgg attgaatttg
     4021 ccactattga ttgagctagt atctcggttg gacgctgaag tagagacaac tgttttgatc
     4081 cagcaggtgc taactgattc aaaaaaaatg atccagtttt gtaacgagcg gctcaatcag
     4141 aaaatagaag aagacgaatt ttaggaggaa gaaaaatgat tgtattgaca agagtcgacc
     4201 atcggttatt acacggacaa gtagcttttt catggacaca aacaataggc gcagactgta
     4261 ttttgattgc taatgatgat gtacctacga atgagatacg aaagacaacg atcaaattag
     4321 ccaagccaca aggagtcaaa ctagtgatca aatcaattga tgactcgatt gccgcgttga
     4381 aaagcggggt gacagataaa tataagttat tcatcgtagt ggaatcgatc gaggatgcat
     4441 ataagctggc agaagcgtat ccagaaatca aaaccatcaa cttaggaggg atcaaggcaa
     4501 aacctggtac tcgcagcatc ggaaaagcgg taaatatttt accagaagag gaggaacgct
     4561 tgaaacgatt aattgacaaa caaatagaag tagaaatccg tcaagtacca gcagataaaa
     4621 aagtggatgt aagagaagtt ttgtaagaaa ggaagaacct caatgattgt acaagcagtc
     4681 ttattaggaa ttgttgcatt tgtcgcacaa tcagaatatg cactagggac atcgttgctt
     4741 tctcgtccga ttgtgacggg gctgtttaca ggaatcgtgt taggtgatgt aaaaactggg
     4801 atcattatgg gcgcaacttt agagttagca tttatcggat cgttctcagt aggtgcttcg
     4861 attccacctg atgttgtgac cggtgggatt ttagggacgg cttttgctat tacagctgga
     4921 gccggtacag aaaccgcgct tttactaggt ttgccgattg ctacgttgac attgatattg
     4981 aaaaatattt acttagggct tttgattcca attatgaacc ataaagcaga caattatgca
     5041 gaagaaggaa attataaagg agtcgagcat atgcacttgc ttgccggtat cggtctttcc
     5101 ttgatgcttg gtgttgtggt aatggtttct tatatggtgg gaagcaatac gatcggaaat
     5161 ctattaaata tgattcctga cttcgttcaa aaggggttgt cagtagctac agggttgatt
     5221 ccagctcttg gttttgcgat gcttgctcgt ttgctgatca acaaacaagt tgcaccatat
     5281 tttttcttag gttttgctat agcttcttac ttagagattc ctgtgaccgg agtagctatt
     5341 tttggtgcga ttttagcagt tgtggtagtc aatattatga atgtccgtca actaccactt
     5401 caaacaactg caggggaggt agaagaagat gaagaattct gagatcaaaa acagcaaaat
     5461 aacgaaaaaa gagctaaatc acgtattttg gcgttctttt caaatggagt tttcttggaa
     5521 ttatgaacgt cagatgaata tggcttatgt ttatgcaatg attccgattt tgaaaaagtt
     5581 atatcagtcg aaagaagaaa tggcagcagc tttgaaacgg catctagagt tttttaacac
     5641 gaccccacat atcgttacac tgattttagg aatcacggct gcaatggagg aagaaaataa
     5701 ggatgatcct gaatttgatg tgactgcgat cgatagtatc aaaacatctt tgatgggacc
     5761 tcttgcaggg ttaggagatt ctttcttttg gggaactttg cgattgattg ccacaggtgt
     5821 gggtacctct ttagccatgc aaggaaatat tttaggccct atcttgttca ttttgatttt
     5881 caatattccg cacattctgt ttcgctatct agcaacaggt tggggatatc gcttaggaac
     5941 gggttttttg aaaaagatcc aggcaaacgg tatgatggaa agtttgacat taggcgcgtc
     6001 tattattgga ctgatggtcg ttggagcaat gactgccacg atgatcacca tcaatattcc
     6061 tttaaaaatc ggttcaggag aaaatgctgt gactgttcaa agtatttttg atgatatcgt
     6121 tccaaatatc ttatgtcttg gagcatttgg catagtgttt tatctattga agaaagaagt
     6181 gaaaccatta acgatcttga tcggactagc tttctttgga atttttggtt cgtggatagg
     6241 gattttttaa aaaaggagca gagaaaatgt ttacgatgca agattatatc tatgaagaaa
     6301 aggaagtact aagtacgatc ttgaaaaaga atgatttttc gacaagagaa catatgaaaa
     6361 aaacagtgaa tttactgatc ttagcaacgg gttcatctta taacgcttgt ttagcagcga
     6421 agccagcatt ggaatcttat ggtgatctta ctgtcgatat tcaagaacct ttttattttt
     6481 tcaattatgg aaaactttct ccttcaatcg atactgtgat tgctgtgtca caaagcggga
     6541 aaagtgcttc tactgttgaa gcagtaaaaa tgatacaaaa acaagggtta cctgttgttg
     6601 ctatcacaaa tgacgtgcaa agtccacttg ctttagaagc tgcacaaatt attgatttag
     6661 gtgccggggt agaatcagta ggcttcgtta caaaaggcta ttctgcgact gttttacagt
     6721 tactactctt agggatagga attggaataa gtaaaaataa aatcagcaaa aaaatagaac
     6781 aagactacat gcaacagttg cgcaaaataa tcaaccattt accagcgatt attcaaaaaa
     6841 cggaagaatt ttttgatgga tatcaaagtt tatttcgttt agcacagcgt ttcgtcacca
     6901 ttggatatgg tccaaactgg ggaacagcta aagaagcaga gacgaaattg actgaaacaa
     6961 tccgcgttcc gtcacaagga tttgagttag aagcttatat gcacggccca tacctggagg
     7021 cagatgcttc gcatttgcta ttctttattg aaggggaaag tgtaaataag gaacgttcgc
     7081 aaaagttgca aagatatatg agtcggtacg ttggagagac tctcaccatt accacaaaaa
     7141 aagcaagaaa cgagaaaaca cttggattag caatagaatg tgatgaatat ctgtctgtat
     7201 tagcgcttgt cgttccgttt caactattcg cttataaaac cgcagtagct aaaggtattg
     7261 acttgaacaa aaagattttt gaagactttg atacagtctt gaaaagtaaa ttataagagg
     7321 tggaaatcat gctgaaattc aatgaagaag aacaaatcaa agcaataaaa ggcgcactag
     7381 ctttacgtcc gcaagtagaa gaaatcatcg ataaacttta tgcggaaaaa tttgacgctg
     7441 tctattatct aggaatcggt ggaacgtatg cttcctcgat gcaagctgtc acttatatga
     7501 atgggaaaag caacttgcct gtctttgtac agcatgctgc agaatattac actactggaa
     7561 acaaaagatt aacaaaagat tctatcgtta ttttgtcctc tgttaccggt acaactcaag
     7621 aagtagtgaa agcagtagaa caaatcaaga aagtaggagc gacactgatt ggatttatcg
     7681 ataaagcgaa cagtaagttg tctcagctat gcgattttgt ggtcacttat ccagctcctg
     7741 gaacagaaca aatcaaattt tttatggcag cagatcgatt gatgtatttg aatggagaat
     7801 tcgaagctta ttcagaatac tatgaacagc tcgaacgtta tttaccaaca gggttagtgg
     7861 aagcagagaa aaaagcagat gcatttggct tatcatttgc agaaaaacat cgacgtgata
     7921 gtatgcatta ttttattgga gcagggaatc aatggggagc tgtttattca tatgccatgt
     7981 gttactggga agaacaaagc tggcttccat cgaaatctat ccatgctgcc gagtttcttc
     8041 acgggacgtt agagatcgta gaagaaacaa ccccagtcac attattttta ggcgaagatg
     8101 aacagagagt gttatctgaa cgtgtggcta aacttttgcc aaaaatttgc agcaattata
     8161 cattgataga cacaaaagac tatccagtag aaggtatcag tgaaaaatat cgcggacgtg
     8221 ttttgtcttt cttattgatg catgtagtga cacaacgcat cgatgctcat gtggagcagt
     8281 tgaactgcca tccattagag atacgccgct actatcgtca gtttgattat taggatagac
     8341 tggataaaaa gaatataaaa aataaagaga gcagcgataa ctgctccctt tattttttta
     8401 aaatattggc atcaattacg ttataaaaca cattttttgc attatctaca gttcaactta
     8461 taatctgttt aatgggacat gtgggtgcca accatttttt gttatgcaat agtactattt
     8521 agttgttgca tggatccgta gtaaaagtcc aagtacaacg agttgcgcca gtttacatag
     8581 cgttttgtat ccagaatgtt gactaaaaca aaagttccta tgtcaaccgt caacagagac
     8641 aatctgtccg ttgcaggacc tgacagattt agaaatgttc aatctgtctc ttatgtattt
     8701 taaatcaata ctcactagga gaagtttcct aacctcctaa tagtaatgct ggtagtaaca
     8761 ttgtcaaaac tgcttcctct taggaacaag caaagcataa cgagtgtaaa aataaaatgt
     8821 agactattgt aacatatcat tgattatttc aagtaaaaac gataaaagaa gtaaacaaac
     8881 acttaaaaac aatggaaatt agtcccaact ttaagaaatg tatactataa tggaaattat
     8941 caattattta tttggaggaa tatccatgaa acaaaaaatc gcagttcttg gtcctggatc
     9001 ttggggcact gctttagcac aaacattggc agaaaacggg catgatgtcc gtatttgggg
     9061 aaatgtacct gaacagattg atgaaatcaa taagtatcat acaaatcagc attttttacc
     9121 tgatttgacg atcccagaat cgattatcgg atataaagaa ttggcagaag cagtggataa
     9181 cgcagatgct attttatttg ttgttccaac aaaagcgatt cgttcagtcg cacaggaact
     9241 tattggtaaa atgaatacta agccaatcat tatacatgca agtaaaggac tagaacaaga
     9301 tacgcataaa cgaatctcag aagttttggc tgaagaaatt ccagaagaaa aaagacaggc
     9361 aattgttgta ttatctggtc ctagccatgc tgaggaagtg gcagtacatg atatcacaac
     9421 aattacagct gcaagtattg atcaaaaagc agcagcctat gtacaaaaac tgttcatgaa
     9481 tgattatttc aggatttata caaataatga tgtgattggc gtagaaactg gtgcagcact
     9541 gaaaaatatc attgcaattg gtgccggcgc tatccatgga ttaggattcg gagatgatgc
     9601 aaaagcagca atcatgactc gaggattggc agaaatcagc cgtcttggtg tcgcgatggg
     9661 tgccaatcct ctgacattta tcggtttgag tggtgtgggg gacttgatcg ttacttgtac
     9721 aagtgttcat tctcgcaatt ggcgtgccgg taacttgtta ggacagggac ataagctgga
     9781 agaagttcta gaaaacatgg gaatggtcgt tgagggtgtg gcaacgacaa aagcagcagt
     9841 agagctagct caacagctag gtgttgagat gccaattact caaacaatct ataatgtttt
     9901 gtataatgga gaagatatca aaaaagcagc aaaagaaatc atgttacgtg acggaaaaat
     9961 ggagaatgaa ttttctcttc gttaaataga tgatcaatag ttaatcgt
//
LOCUS       DAJSBM010000082         9981 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJSBM010000082 DAJSBM010000000
VERSION     DAJSBM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.083"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            201..680
                     /locus_tag="ONT67_002428"
     CDS             201..680
                     /locus_tag="ONT67_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356144.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            713..2941
                     /locus_tag="ONT67_002429"
     CDS             713..2941
                     /locus_tag="ONT67_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356145.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3070..4713
                     /locus_tag="ONT67_002430"
     CDS             3070..4713
                     /locus_tag="ONT67_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR2356146.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4786..5331)
                     /locus_tag="ONT67_002431"
     CDS             complement(4786..5331)
                     /locus_tag="ONT67_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCR2356147.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5354..5839)
                     /locus_tag="ONT67_002432"
     CDS             complement(5354..5839)
                     /locus_tag="ONT67_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2356148.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5829..6641)
                     /locus_tag="ONT67_002433"
     CDS             complement(5829..6641)
                     /locus_tag="ONT67_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCR2356149.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6780..8312)
                     /locus_tag="ONT67_002434"
     CDS             complement(6780..8312)
                     /locus_tag="ONT67_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCR2356150.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8421..9452)
                     /locus_tag="ONT67_002435"
     CDS             complement(8421..9452)
                     /locus_tag="ONT67_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2356151.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3318 a         1843 c         1920 g         2900 t
ORIGIN      
        1 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
       61 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
      121 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
      181 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
      241 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatatggat gcaaaagggc
      301 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
      361 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
      421 tagatttagc gatcttagct gcgcaaggaa gtttatactt ccaagatgct tatcgttatg
      481 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
      541 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
      601 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
      661 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
      721 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
      781 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
      841 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
      901 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
      961 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
     1021 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
     1081 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
     1141 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
     1201 cccttctgaa atcgttcgag cgattgaaaa aggctattta ttagagattc aagagccgac
     1261 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
     1321 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
     1381 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
     1441 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
     1501 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
     1561 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
     1621 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
     1681 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
     1741 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
     1801 tacaaaagaa agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
     1861 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
     1921 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
     1981 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
     2041 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
     2101 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
     2161 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
     2221 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
     2281 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
     2341 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
     2401 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
     2461 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
     2521 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
     2581 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
     2641 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
     2701 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
     2761 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
     2821 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
     2881 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
     2941 ataaaataaa gctcagcgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
     3001 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
     3061 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
     3121 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
     3181 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
     3241 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
     3301 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
     3361 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
     3421 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
     3481 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
     3541 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
     3601 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
     3661 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
     3721 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
     3781 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
     3841 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
     3901 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacatgca
     3961 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
     4021 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
     4081 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
     4141 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
     4201 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
     4261 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
     4321 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
     4381 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
     4441 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
     4501 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
     4561 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
     4621 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
     4681 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
     4741 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
     4801 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
     4861 tttcaaacga ggtctgacag gtacttggac atgcttgata tgcataccga ttgatgtgtc
     4921 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
     4981 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
     5041 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
     5101 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
     5161 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
     5221 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
     5281 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
     5341 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
     5401 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
     5461 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
     5521 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
     5581 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
     5641 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
     5701 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
     5761 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
     5821 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
     5881 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
     5941 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
     6001 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
     6061 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
     6121 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
     6181 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
     6241 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
     6301 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
     6361 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
     6421 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
     6481 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
     6541 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
     6601 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
     6661 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
     6721 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
     6781 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
     6841 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
     6901 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
     6961 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
     7021 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
     7081 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
     7141 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
     7201 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
     7261 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
     7321 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
     7381 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
     7441 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
     7501 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
     7561 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
     7621 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
     7681 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
     7741 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
     7801 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
     7861 gcaggttgtt ccattaaata aagaatagga atctgttttg attttgttcc atttgaaatt
     7921 tctctcactc gcttttttgt ctcatcatca aataaaatga aagggtcttc taaacgatcg
     7981 ataaatactt ccattgttgt tgttggtaga tgataagaag tcatgacagg cactgcacca
     8041 aggtaagaca cagaaacagc tagaaggtat gtgtcaaaag cggaactctt ataaataata
     8101 actttgtctc cggatttcac tccaagatga gccaattgat aagcccgttt caacacactt
     8161 tcgcagcatt ttttgtaagt tgtttcaagt cctaattctg ggaaagctga aagtggctca
     8221 tcaaagataa tcggggtctc ggaaaagtta gctgcagctt cttgaaaatt cgtaaataag
     8281 ttcaaagggg aatacgttat ggtctccatc attcacactc cattatcttt tgttaagtaa
     8341 tatacaaatt caagataatc gtaacataat cctttatata ataacatagt ttattgtgaa
     8401 aaaaagatca tacatttgtt ttatatggaa gaaacccgaa cgaatgccgc taattcgtga
     8461 taagaaaaat caaccatctt ttcccagata tcttgaaaag aatgggatga gaggtatcga
     8521 ttaccacata aataatttac cgcgtcagca tcatctaaat atagttctgc ataataacga
     8581 tatgtttttc tgcatagttc tggcgatacg tttttatata ctgtaatttt gctcaaaatt
     8641 tggcgaatag tccgaggaga aatttgctga tcatgtgagg aacgaaaaac aaaagaattt
     8701 tcgtccgtat tttgtgctgt taacttaaaa tagttctcca gaaatggcat cgcttctttt
     8761 ggtattggta ctttattcag tctgttttct tttctattca gattaagaat ctgcttggtc
     8821 aaagatacat ctttacgttt aagttctact acttcatgga tctttaatcc gcaatacaga
     8881 agaatggcag taattgcaag gttcctcggt ctattctttt cttcaaagct agcttctctg
     8941 cttgtttgaa agccttcaat cgggataaac aaatgctcaa aatctttgaa aagttctgca
     9001 tcgatatatc gcggagctgc tttggttgtt tccggtaaac ttgtacccaa aagagaaatc
     9061 tcttcttcaa tcagtccttt ttgatacaaa aatcgtaaaa aatgatttaa tgaatattgc
     9121 ttccttgaaa tcccgtaata gctattttca aacaattgct ccgtttcttg gttcgctgta
     9181 gtttggaacg aacgagtatg tcgatagatt ttagctaaga aattctgata atcttgtttt
     9241 tctaaatgga taatcagctg atcatccact tcttcagaaa atacttttct caaataatca
     9301 gtaaacagat gaaaatcttt tgcgtattca tagcgactgc ctaatgaaag ggaagaacga
     9361 ttttcttcta aaaactgttg aaagacggaa gggagcaagt gtgtacgttg ctttaacttt
     9421 tcttgcagtt gttttctagt tttgctgtcc attacaaatt ccctccaata gtgttcattt
     9481 tctatatttt accgcatttc atcaaatgaa gatagacagt ttgtcttgat cttaggaagc
     9541 aaatttttaa ctaagtacta gctaaaagat atgaagtaag aagatacatt tttagttatt
     9601 ttattagtgc agtcattctt ttgatctttt aattgattta tcctcttttt agctatgtgg
     9661 ttacttcatg agcaatgaaa tggatttggt tcgttctaat ggcaggaggc tctttctttt
     9721 atttccgaaa aattagaaaa cattttttca gaatcgattg gctctccatt tattcaataa
     9781 tggtttgtct aacgatattg ttgggataat tcttaaagaa gtattagaaa tagttggtac
     9841 ttcattactt tacatgaaga tttattctat ggtaggagca attatattga gaattaatcc
     9901 ttttttatat aagttgtttg cttattgatg gattggataa attaaaccat cacaacgagt
     9961 ggaaattata tgaaattttt c
//
LOCUS       DAJSBM010000083         9626 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJSBM010000083 DAJSBM010000000
VERSION     DAJSBM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.086"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..563
                     /locus_tag="ONT67_002436"
     CDS             <1..563
                     /locus_tag="ONT67_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCR2356152.1"
                     /translation="KHIDVYQKHDYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRH
                     HKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFK
                     NKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVR
                     KVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            556..1983
                     /locus_tag="ONT67_002437"
     CDS             556..1983
                     /locus_tag="ONT67_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR2356153.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1985..3055
                     /locus_tag="ONT67_002438"
     CDS             1985..3055
                     /locus_tag="ONT67_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356154.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3042..4463
                     /locus_tag="ONT67_002439"
     CDS             3042..4463
                     /locus_tag="ONT67_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2356155.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4476..5483
                     /locus_tag="ONT67_002440"
     CDS             4476..5483
                     /locus_tag="ONT67_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCR2356156.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            5495..6499
                     /gene="neuB"
                     /locus_tag="ONT67_002441"
     CDS             5495..6499
                     /gene="neuB"
                     /locus_tag="ONT67_002441"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HCR2356157.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6496..7644
                     /gene="neuC"
                     /locus_tag="ONT67_002442"
     CDS             6496..7644
                     /gene="neuC"
                     /locus_tag="ONT67_002442"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HCR2356158.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7617..8294
                     /locus_tag="ONT67_002443"
     CDS             7617..8294
                     /locus_tag="ONT67_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR2356159.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            8284..9426
                     /locus_tag="ONT67_002444"
     CDS             8284..9426
                     /locus_tag="ONT67_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR2356160.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            9439..>9626
                     /locus_tag="ONT67_002445"
     CDS             9439..>9626
                     /locus_tag="ONT67_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2356161.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEI"
BASE COUNT         3456 a         1206 c         1715 g         3249 t
ORIGIN      
        1 ataaacatat agatgtatat caaaaacatg attatcctag aattaatagg aataacacaa
       61 ctgcatttaa aatttttaga gctataatta atcgttgtct gaggatgatt aaacttcccc
      121 caaaaattag acaccataaa tttgatttgt atcatggttc tcaatggtgg gctttgtcga
      181 aagaatgcct aacagaatta atacaaatgt atgaacaaaa tcaacaagac tacctgaatt
      241 ttaaaatagg tatgtttgcc ccagatgaaa aattttttca tacacttttt tttaattcct
      301 cgttcaaaaa taaaaatgtt attggaggac cagatttacc acttgaattg aaaaatattg
      361 aagaaactac tttacaaact tcgaaattag caaatattca tattattgat ccttccatga
      421 acaaatggtt tgatgaaagg gatatagatc aaataaaaga aagtgaaaaa ttatttgtaa
      481 gaaaagttaa atcggattac tctgataaac tattaaatag aattgataaa gaaattttga
      541 aagttggaaa tatagatgaa tagaatagta cctgttttag agaagagttt attaagtcga
      601 gatgcgtttc gagtaataaa tttaatcatt tgtctgcttt attttatgcc tagtactttt
      661 aatgtaacta atatcccgat gaaaatagct tttgcttggg gtggaattat agttttgtat
      721 gaggttttta taaagaaaaa tttttttaaa atgcgttttt cttggctact gattggagca
      781 atgattagtt ttatcttaac tataatcatt aacagagaaa attttttaat tccatctatt
      841 tataattttg gatatttgct gattactttg atagtaattt ttcctgcaga cttttcacaa
      901 agtgaagagg aaaaaaagaa aaaaatggtg tattttaata atgttttcat tatcataatt
      961 tttttagctg cgctaatatc tatctatcaa tttatttttt tgatttctta tcatgtccca
     1021 acaggaacgc caggattact tgcaagacaa ggttttattg aacatcggtt atttggtgtc
     1081 tatacaagtc caaatgttgg agctatgttt ggatatactt ctattatttt aagtttaatt
     1141 agtgtattaa tttcaaaaat aacaaaaacg attcgtgttt tttatatgat gaattttatc
     1201 gtacaaatat tttatattac actttcttcg tctagaggtg cccaggtagt aatttgttct
     1261 tttttagtat tatttctttt gttgtttgcc aattcaaaat ttagaaagag cattagagaa
     1321 gtactacctg taaaaatcaa atggatggta agcatattat tagttctatt ggtatatttt
     1381 tcttttggta ctaattatgc taaggatttt ttagcaacaa ttcctgtatc gattgataga
     1441 aaagtagaag ttatagagtc aaatgaatca gataaaactc cacttccaga aaaagaagta
     1501 atattacagc attctagtga taactccgaa atttcagctg gtcgttttac gatttggaaa
     1561 gctgcgctta aagttataag accaactttg ttatttggtt atggagagac cgatttctat
     1621 gggaatagcg atgcaaaatt tttgaaaaaa gtagatcttg atactacaga tgtgaatgaa
     1681 ctaaaaagag cacatggaaa catgcataat ggatttcttc aagtacttgt atcaagtggt
     1741 ataattgctt ttatatgtat ctatgctttc tatgtgttaa atatctttag tttactcaaa
     1801 acatttttta gaagtaaagt gaattatgcg tttcttggta ttgttttaat ttttatacta
     1861 tctattttta tagatgaaat ggtggaggct catgtacttt tcaataagcg cgatgtcatt
     1921 tcaattgttt tctggtatta tttgggaagc atttcttact tgtatttaaa aaaggagaat
     1981 taatatgaag acagctttca ttgcttggac tcctttacat ttgattaata tattaaatgt
     2041 ttatcaaaat ttttaccgag aatcaaaagg agatttattt atttattctg aatttgaaaa
     2101 cgctagaaga tatagtgagc gtataaaaga attagggcta tttaatgatg ttgtttttgt
     2161 gaatcaccgg aaaatcggtt cttcctttaa acgtaaaatt aacttgttaa ctaaccataa
     2221 tcaatttttc tcggatgatt tatccgttta tgatgaaatt tttatacagg gggaaaatta
     2281 tttcacaaaa atacttttct ccaatgttta caaaaaaaat ccagatgtaa aactaaatta
     2341 tatagaagat ggtttgggta cttatttgaa gaagagtata ttttcaacaa gtaaaaacgt
     2401 tcaaagagtt tttaaaatat taaataaaaa tagcatcttc aaaaaaaaaa ttgacgatta
     2461 ttttgtctat tgtccggaat tgttacttta tgatgtaaaa aatataaaaa aaattcccaa
     2521 aatagatgaa aacagtaaat tgatgaaaaa catacaatca atctttggga tagaagaagt
     2581 ttttgtcgga gataaatgta tcttttttga tcaaccttta ttatctgatg gttatggcat
     2641 agatgaaaca gatattttcg ctaaagtaaa tagtatattt tctggaaaaa aagaaacttt
     2701 aattaagctt catcctagag caaggacaaa tcgttatggt gaaaaaaaca tattgaattc
     2761 cagtttaccg tttgaagtct tgctacttga aaatgacttc tcttcaacac ttttgattag
     2821 tcctatatct acaatttctt tttcaccatc tatgatgttt gaaaagacga tcaactctat
     2881 tatgttggct aaattaattt taaatgaata tacagcatta tctgaagata aaaaagaaac
     2941 attgacaaat attgttcttt tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt
     3001 actcccagaa aactgggcag aattaagaaa gttggtagag tatgggatta tttaacacac
     3061 tttccaagtc aaaattagtt aagtccagtt tctggtatac tatagggaat ttttttatta
     3121 agggaatttc attcataact attcctattt ttgtaaatat tactactcaa gcagaatatg
     3181 gtttgattaa taatttcact tcatttgtat caattttttc gattcttgtt gggctaagcc
     3241 taaattcggc aattaataat gcaagttttg actataaaga tgacataaat gaatttatgt
     3301 catcggtact ttctctagct acactttcgt taattgtgtt cttattaata gggaacatat
     3361 attttttgtt ttcaaatgct ttttttgata tgaaccaact tgtatttaat ttgatgttat
     3421 tacaaagttt tgctagtttt gtaattagtt atatccagtc ttattttacg attaacttta
     3481 accattttaa atatttgatt ttatctttta tatctacttt tttgaatatt gcaatctctg
     3541 tagctttgat gcagacatta tattttgatg ataaatatgt tggcagagta gtcggcggaa
     3601 cactgtcatt tgtgttgata ggtttagtaa tttatttaag aataatgata aagggtgctc
     3661 atttttttga taaaaaacag tggagatacg ctttaaaaat ctctttgccg ctaatcccac
     3721 attccttagc aaatatagtt ttagcacaat tcgatagaat tatgattaat tcttatttag
     3781 gtcctgctgc ggggggaata tattcctaca ttagtaacat aggtattatt ctaagcgtaa
     3841 tatggatgtc tactaataac gcctgggttc catggttcta tggtgagatg agtaaaaata
     3901 atgaaaaaaa agtgaagaaa gttagtaact attatttagt agtctttact ttgattacga
     3961 tgattgtaat ggtagtttcg attgatttag caaaatttat ggctccgaaa gactattatt
     4021 ctgggttatc tttagtggtt ccgataacct taggctattt ctttcagttt ttatatagtt
     4081 taccagttaa tgctgaattt tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag
     4141 caagtgctat agttaatata ctattaaaca tgattttcat acctaggtta ggaataattg
     4201 ctgctggata cacaaccgta tttacgtatt ttttactttt tatttttcat tataatatcg
     4261 ctaaaaaaat tacgaataaa caattatttg acacaaaaaa aatatgtatc ataataattt
     4321 ttgtatcaat tttttctttg atacttcata ttttaatcaa tcaaatgttg attcgttacg
     4381 ttttgacatc attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa
     4441 ttatagatga ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag
     4501 taactggcgg aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac
     4561 caaaagctat cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc
     4621 tctcaaatca cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg
     4681 attatgcatg tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag
     4741 caagtgaata taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa
     4801 tagatgcagc gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag
     4861 tgagtcctac taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg
     4921 ccaatcagta taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca
     4981 tggggtcaag tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata
     5041 ttactttgac agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat
     5101 tagttctgtc tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg
     5161 caatcaatat tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg
     5221 aaaaatatag aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag
     5281 agctaatgac tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc
     5341 caccacacta tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg
     5401 catactcttc agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag
     5461 agaataatgt cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc
     5521 agaaattggt gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc
     5581 taaagaatgt ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc
     5641 caaaaaggct ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt
     5701 tgaaatgcta aaaaaattag aattatctca atctgatttt attagaataa aagattattg
     5761 tatacaaaat aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct
     5821 aaaacaaatt ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt
     5881 tcttagaaaa atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat
     5941 tcatgaggtc agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg
     6001 tacttcaaat tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa
     6061 agaaatgttc gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat
     6121 ttgtgctgtt gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact
     6181 acctggtcca gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca
     6241 tattagaaac actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact
     6301 tagtacaaaa gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga
     6361 tattcttaca gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt
     6421 tttatatcaa tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct
     6481 tgaggaagat gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga
     6541 atatataaac ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt
     6601 tctggaatgc atttatccca taaatatgga tttactaaag aattgatctt agctgacgga
     6661 tttagaatta gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct
     6721 cggtctgtgg cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat
     6781 tttgtattat tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat
     6841 cttggaatca aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca
     6901 ataagacatg ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa
     6961 gagcgtttaa taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga
     7021 atagatacta ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat
     7081 ctaaaatttg aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta
     7141 gaaaatatag aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca
     7201 attatttgca ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag
     7261 aaatatttaa aaacggatat tatttatatt acgaatttag agccggagga atacctgact
     7321 atattaaaag agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca
     7381 tcattcaaaa taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa
     7441 aatattataa atgcaaatct aaatgaaaat gaaattgaaa ctgctttgaa taaaatacaa
     7501 gatcctcagt ttataaaaag cttgaaaaat gttaagaaca tctacggtga cggcaacagt
     7561 tctagaagaa taacaaaaac attgaatcaa atattgaatg atggggaaga tattaagtgg
     7621 attcaaaaac aaatagctta ttaaaatata ctagtgatcc atacattatt attggggctg
     7681 gcggacacgc aaaagtcatt attgaaacct taagattaga aaataagtta atatatggat
     7741 tttttgatga taagcaaaat aaaatgcaag atctgaaata tcttgggaaa ataaacgaat
     7801 tgaatagctg ggcggatagc aaaaaaaaaa tctataaatt tattgtagct attggtgact
     7861 gtgtagtaag aaaaaagatt ataaaaaaat tgaatatgcc aagtgagcaa tatggaatag
     7921 ctgttcatcc aagtgctata gtggcaaaag aaatttcaat tggttatgga actgttattt
     7981 ttgcgaatgc tgtaattaac ccagatgctg taattggaga gcatgctata ataaacacag
     8041 gtagcattgt tgagcatgat tgtagaatta ataactatgt tcatttatct ccaggtgtat
     8101 gcttagcggg tggagtacat gtaggtgtag gaactcaaat aggaattggt tctcaatgta
     8161 tccagatgaa gaaaattgga tcttggtgta tgataggcgc agggagcact attgttaagg
     8221 atattccctc acattcctta gcttatggca atccagctaa aattaagaaa gaaggaatta
     8281 attttgaatt ctagaatttt attatcaagt cctcatatgg gaggaacaga agaaaaatat
     8341 gttgctaagg cttttaaaga taattggata gcgccattag gtgaaaatgt ggataaattt
     8401 gaagaaagta ttacaaaata tactggttct aagggcggag cttctgcgtt aagttcaggt
     8461 acagctgcca tacatcttgc cttaagactg cttaatgttg gaaaaaacga tattgtcttt
     8521 gctccttcat ttacttttat agctacagta aatcctattt tatatcaagg ggctgtacca
     8581 gtgttcattg actcagaacc taatacatgg aatatgagcc ctgttgcact agagagcgca
     8641 ttagaagaag ctgaaagaaa caaccaatta cctaaggcaa tcatcgtagt gaatttatat
     8701 ggtcaaatgg ctgattatga acgaatttta gctattgcaa accaatttaa tgttccagtg
     8761 atagaagatg cagcggaatc gttaggttct acttataagg gaatttcgtc aggcttattt
     8821 gggacaattg ggatctattc atttaacggt aataagatca tcacaacctc aggtggagga
     8881 gctctagtgt ctagtgataa aaaatatgtt gagcattcaa gatttttagc aacacaagca
     8941 agagatcaag caccgtatta tcaacatagc gaaataggtt ataattaccg tctaagcaat
     9001 atttctgccg gtatcggacg tggacaaatg gaagtattga atgatagagt agctaaacga
     9061 agagaaattt tcaatcgata taaggaagct ttttctggaa ttgatagaat tgaaatgatg
     9121 ccagaagtgg atggcatggt gccaaatcgt tggttgtcta ctttaacgat caatgattat
     9181 aattctattg atcaagtagt taacgtaata aataaactaa atgaaaaaaa cattgaaaca
     9241 agagctttgt ggaaaccact acatttgcaa ccagtatttc aaggggtaaa aatgtattac
     9301 catgataata atcttagtgt gtcagaaaag ttatttaata gcggcttatg cttaccttct
     9361 ggaagtaata tgacagatag tgaacaagaa tttgttattg atgagtttaa aaaaatattc
     9421 aagtaggtgt gatattttat gaacatagga attattggtt taggttccat ggggaaaaga
     9481 agacttagac tcattcaaga acactttaat gatattttac tatgtggtgt tgatactaat
     9541 gaggcgagac gaaaggaagt tgaaaaggaa tttggaatac caacttataa tagtctaaca
     9601 gaggcaaaaa atttgtttga aataaa
//
LOCUS       DAJSBM010000084         9361 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJSBM010000084 DAJSBM010000000
VERSION     DAJSBM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.087"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(92..760)
                     /locus_tag="ONT67_002446"
     CDS             complement(92..760)
                     /locus_tag="ONT67_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCR2356162.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="ONT67_002447"
     CDS             894..1181
                     /locus_tag="ONT67_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356163.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="ONT67_002448"
     CDS             1597..3135
                     /locus_tag="ONT67_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCR2356164.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="ONT67_002449"
     CDS             3132..4097
                     /locus_tag="ONT67_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2356165.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="ONT67_002450"
     CDS             4084..5406
                     /locus_tag="ONT67_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2356166.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="ONT67_002451"
     CDS             5647..6390
                     /locus_tag="ONT67_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2356167.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="ONT67_002452"
     CDS             6405..7397
                     /locus_tag="ONT67_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR2356168.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="ONT67_002453"
     CDS             7409..8146
                     /locus_tag="ONT67_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2356169.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="ONT67_002454"
     CDS             8136..8858
                     /locus_tag="ONT67_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCR2356170.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="ONT67_002455"
     CDS             complement(8942..>9361)
                     /locus_tag="ONT67_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCR2356171.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DAJSBM010000085         9290 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJSBM010000085 DAJSBM010000000
VERSION     DAJSBM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.088"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(138..302)
                     /locus_tag="ONT67_002456"
     CDS             complement(138..302)
                     /locus_tag="ONT67_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356172.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            597..1925
                     /locus_tag="ONT67_002457"
     CDS             597..1925
                     /locus_tag="ONT67_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCR2356173.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2127..2960
                     /locus_tag="ONT67_002458"
     CDS             2127..2960
                     /locus_tag="ONT67_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR2356174.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2976..3713
                     /locus_tag="ONT67_002459"
     CDS             2976..3713
                     /locus_tag="ONT67_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCR2356175.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3868..4794)
                     /locus_tag="ONT67_002460"
     CDS             complement(3868..4794)
                     /locus_tag="ONT67_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR2356176.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5069..5902
                     /locus_tag="ONT67_002461"
     CDS             5069..5902
                     /locus_tag="ONT67_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR2356177.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5936..6775
                     /locus_tag="ONT67_002462"
     CDS             5936..6775
                     /locus_tag="ONT67_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2356178.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6801..7361
                     /locus_tag="ONT67_002463"
     CDS             6801..7361
                     /locus_tag="ONT67_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2356179.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7560..9281
                     /locus_tag="ONT67_002464"
     CDS             7560..9281
                     /locus_tag="ONT67_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCR2356180.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
BASE COUNT         3162 a         1560 c         1909 g         2659 t
ORIGIN      
        1 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
       61 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      121 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      181 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      241 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      301 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      361 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      421 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      481 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      541 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      601 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      661 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      721 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      781 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      841 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      901 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
      961 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1021 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1081 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1141 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1201 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1261 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1321 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1381 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1441 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1501 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1561 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1621 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1681 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1741 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1801 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1861 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1921 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     1981 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2041 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2101 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2161 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2221 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2281 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2341 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2401 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2461 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2521 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2581 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2641 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2701 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2761 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2821 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2881 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     2941 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3001 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3061 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3121 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3181 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3241 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3301 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3361 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3421 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3481 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3541 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3601 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3661 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3721 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3781 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3841 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3901 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     3961 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4021 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4081 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4141 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4201 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4261 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4321 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4381 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4441 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4501 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4561 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4621 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4681 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4741 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4801 cgttccgccg gtgtgtagta caagtatttt ttttcatttt acagacttcc ttacgaataa
     4861 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     4921 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     4981 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     5041 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     5101 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     5161 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     5221 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     5281 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     5341 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     5401 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     5461 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     5521 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     5581 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     5641 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     5701 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     5761 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     5821 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     5881 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     5941 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     6001 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     6061 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     6121 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     6181 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     6241 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     6301 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     6361 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     6421 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     6481 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     6541 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     6601 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     6661 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     6721 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     6781 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     6841 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     6901 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     6961 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     7021 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     7081 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     7141 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     7201 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     7261 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     7321 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     7381 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     7441 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     7501 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     7561 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     7621 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     7681 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     7741 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     7801 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     7861 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     7921 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     7981 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     8041 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     8101 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     8161 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     8221 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
     8281 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
     8341 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
     8401 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
     8461 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
     8521 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
     8581 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
     8641 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
     8701 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
     8761 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
     8821 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
     8881 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
     8941 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
     9001 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
     9061 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
     9121 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
     9181 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
     9241 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa
//
LOCUS       DAJSBM010000086         9263 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJSBM010000086 DAJSBM010000000
VERSION     DAJSBM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.089"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..299)
                     /locus_tag="ONT67_002465"
     CDS             complement(<1..299)
                     /locus_tag="ONT67_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCR2356181.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTF"
     gene            complement(289..2463)
                     /locus_tag="ONT67_002466"
     CDS             complement(289..2463)
                     /locus_tag="ONT67_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HCR2356182.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2479..4821)
                     /locus_tag="ONT67_002467"
     CDS             complement(2479..4821)
                     /locus_tag="ONT67_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356183.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4843..7386)
                     /locus_tag="ONT67_002468"
     CDS             complement(4843..7386)
                     /locus_tag="ONT67_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356184.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7396..7794)
                     /locus_tag="ONT67_002469"
     CDS             complement(7396..7794)
                     /locus_tag="ONT67_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2356185.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7875..8159)
                     /locus_tag="ONT67_002470"
     CDS             complement(7875..8159)
                     /locus_tag="ONT67_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356186.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8183..9181)
                     /locus_tag="ONT67_002471"
     CDS             complement(8183..9181)
                     /locus_tag="ONT67_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2356187.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2633 a         1989 c         1449 g         3192 t
ORIGIN      
        1 aaaaaagttt gaagttcttc ttgtcttggt tcttttcttg tttcaattcg atatacattc
       61 ctagagaatt ttttgtcgta tctttccaag aaagattcaa cttcttggca ttttttacaa
      121 tcatttcttg ctagatacac aaaaaatgat tgattcgttt tttgaagttc tagtacttct
      181 tctaaattac tattgattag ttttccattt gattcttcgt tagtttttat cagtttaaaa
      241 caaccaaata agaggaaact aacaataagt atcctaacaa taactttctt atttcgcata
      301 ttgtttctcc aaatttggtt tccgaatata gacgactcgc ggtggttcct ggacgtcttc
      361 agctgttcga tacaccacaa attcgcctgt tctcaatgct cgaatgcgat tcggattgaa
      421 tttaaagcgg tctacgtctc gtttcgtgcc tctttctcct gtccaatcaa cctttcccat
      481 atcagaataa cctgcttctt gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc
      541 cattaaatct gcccaataat caatttctgt cgggtcattt gtcttcccaa taaagaacgt
      601 attcacattc ccaatcaact gttttcgaag gacaggatca attctatcaa tgtccgcaag
      661 cgtttgaggg gcatacgccg catgcacccc agcgcctcgt cccttattga ctaagtctat
      721 aaggttatca tttagataag aagctggttc gtcaaatatt actaggactt tcgagcgatt
      781 ggctgtttct cctgcttgtt tatacacttc tgacgcaaag aaattaatat ctcccacaag
      841 catttgagcc aatgtccgaa taaactcact ataaatcaat ccgtttaacg agatataaac
      901 aatctttcct tctttaacaa cttcttgaat atctaattct ctttttcctc cagaagtatc
      961 aaagagttca ccaagctcac tatccaataa catattgata ttggtctgta aattctcaga
     1021 agtcgcatac aaataaaacg ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa
     1081 caacgtttgc tcgatttctg tcatgagaga aatgttttct tttaataata gataatacgt
     1141 ttctaagtca acggttttac gatcatgttc aacgattcgc tcttcactta actgttccac
     1201 atcggcaaaa tcacgttctg ttttgtccat cgcttcattc tttttcgttt ttttcttcgt
     1261 ggttttcttc ttttctacga ccactttttc tttaaataaa tctggatttt tcaactgaat
     1321 acgatccgca aacaaatgta agacgtttcg tggcaataaa taatgttgta accaaactag
     1381 atttctttca attttatggc tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa
     1441 taacgctttg gctgctgtcg aataatacac gctttctgtt tctgctagat tggttaattt
     1501 atctcgaatg gcaatatcat tgccattttt tacaggatta taacgcaaat cgccaccgtc
     1561 cacaaagact tcaatcgggc gattgtatgc ctctgccact tcttttaacg cttgtaaagt
     1621 ctctcctgct cctttcccat cgactaagat aacggggata cgaaacttac acgcatgttc
     1681 tgctagtaat tggatcaccg tcgtttttcc tgaacctgta gtcgctggaa taatcacatg
     1741 ttggttcaat tcactaaagt tcatcacata agggagccca tactcatctg ttcctaatag
     1801 aatcactttg gctaatgaag ccagttgttt attccgcaaa gcaacgtctt ttatgacaat
     1861 tgctacctgg tacgcttgct gatactctgc tgtatatttt tgggactcct gaaatttatt
     1921 cttccgacgt tccttgtatt ctttactatt tagcaaagct gtttgccgtt ctttctttga
     1981 aacaactttt cttttttcaa taaaagacgc atacgcaatc aaacaacttc ctgcacctgt
     2041 cccacctaaa aggagggcaa aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa
     2101 gtttaatgga aataataagt aacacgacaa ataccctcta ggaagaacaa aaataaaaca
     2161 aaatagattt aatagcgtaa aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt
     2221 taagaagctc tcatactcaa agtcataaat atgtgcacgt gtctcttttt tatctatcat
     2281 aaagacaatc gctaaaggaa tactgcctac aataaaaaat ggatatagac aaacacctaa
     2341 taaagtgacg ataaaataaa gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt
     2401 tcttttgcga tcgacagaaa acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat
     2461 catactatcg atttcctcct atcgttttcg ttgtttcatt tctcttcgat aaatcggttg
     2521 tttttctcgt tttcgtttca tttttgcttg agttgctcga aactcactcc gtgtcttcgg
     2581 tgcttgtttt ggtgattttg tttgtttcat ggttgagtat gctttagata cggttgaagg
     2641 cgctttggtt tgtgctgtta ctcgattgac tgctgacata tgttcccgaa ccccctctgg
     2701 acgtcgaaca gatttcggtg tggtagaacc tgttgctcgt ggtacattcc cttttcttag
     2761 tggtttttgt accgcatgtt tttgtcctgc aactgtccgg gttttcatcg aacttgtttt
     2821 tgttacggga gtaagtgcct ccttccgacg acgacttgtt tgtccctgac gacgttttcg
     2881 atcgttcaat tctgttctag gcgtatgctt ctggccataa gacttgtgtt tttgctttgg
     2941 attcgctaat gacaccacca ttgcagaacc ctgattgcga tacggactac gagacgcacc
     3001 ttttctactt ctttctctta gactaggatt gccttgcaat tctcgttgta ataaatcggc
     3061 tgtttttctt gcctgtttct ccattgctgt tcgttgcata cgttcttgac tttgctcttc
     3121 tgcttgtttc gcttgatcat gcaagtctct tgctttttgt gccatacgtt ccatacgatt
     3181 tggtgcttta tgacgaggtt gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg
     3241 tttcgccgtt tgtggcatac gaacgtctga gccaccccca cgttttcgtt gacgttcttg
     3301 tctcttccgt gcgttttctt tttctttttg tttctgtgct tttctcgctt ctttgcgttc
     3361 tttcaaccgt tgtttggctt cccttctcaa ttttgcttct gccccaaatg gatttcccgc
     3421 aaaagctaac gctcctcgaa cacccattct tccagtaaat agactgccta aaaagcctcg
     3481 gaacaaataa attcccaacg gcgcaaacaa ataaacaatg atcaagagaa ttttttccca
     3541 tggatcgtct tgtgtactac ttaattggaa cccgtaacta agaaaagaag aaaaaaggat
     3601 acatgcaaaa cctgtcattc cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt
     3661 accaaacaca ttcattgtga caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg
     3721 tactaacaat agttgcatag ttccaataat caatcgaaac atacttaaga caaaataaac
     3781 aacggtttga atcagattca cgacaaaata aaacaaggca aatgctgtat ttttccaact
     3841 attgtaatat tgtatgttct tgtttttcag tgtatctgct tcatactctg tgactttgtc
     3901 atgaattttc tcccctattt ttgtacctaa atcattgtcc aataaaagat tgattcgatc
     3961 atattctttt ccagcatatt tgactttctt ctcacgaatt gtgtctacat tggttgtgcc
     4021 ataatttaac aacagatatg gttcataaac attactataa aagacccgag ccgccatcaa
     4081 tttgcctgcc gtttccactt ttttatccgc tgttacctct tctgtttctt ttgtttcata
     4141 atttgtttct tcttcaggtg ttaacactgg attgaccttc acgaacgccg tttccactgt
     4201 agaatctaaa gaaaaaagaa catcaaaaag attcttattt gtcccagaat cttttaatac
     4261 gcttaaccca gtaaaaataa ggatcgtcat aagaaagaca cggagtgctt gtttcattcg
     4321 agcctctcca gcatatttga ttgccatcat aattgccgaa aaactgattc caatagttcc
     4381 tgcaatactt aacatattgt ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat
     4441 ggcggaaata atatctaatt caaagagaaa ttcaaccatt gaagcattga attctcctaa
     4501 catttttacg ccactccaaa caaaattttt catcactcct gcaacattgt acgatagctt
     4561 ttttttaagg ttcattgact catagtcatt ggtcatcaat tcaaacgaat tctttttgta
     4621 atttttatac aattgatcca catcagtcgt tacttctttt acttctactt tatcgttggc
     4681 attctctcct tcagaacgat cggtttcagt gacacccgca ccatcattca tttgatcggc
     4741 agaaacagta gccccttgta gacagaaaaa aagaccccct aataaaagga gcgtccacca
     4801 tttcagagat ttttttcgca aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt
     4861 ttctaacatt gcttgaattt tttcatcttg attttcagta gaagaaacag ccaataacca
     4921 actatcaaac aacacatcaa tggcaatcgg ttgatttctt cccaaatgat cctggaataa
     4981 acaggttcct gtttgtaatt gttccatcat ttctaaattg gctgaattca cttccatatc
     5041 aaagaaggac aatagtttct ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta
     5101 agaaattaac tcttttttat cttcttgatc gtaatcttta aacgcttgca caacaaggta
     5161 aatatccgta ttattcgcac gtccttttcg caagctatca tcaatcaaat agcttccttg
     5221 tgcggtatct tcataccctt ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt
     5281 tgtagaaaag atatacgtaa acttcataat gacttccata attgccattc ctgcaagttg
     5341 ttcgctattt aatcgacccg aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt
     5401 ccctaacacg ttgatttgtg aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag
     5461 aatctttcct aaaccaacgc tatgccctaa aatcaattta gctagttctt tgtcactttt
     5521 tcgaatttct tcgataactt gacttaacga atgcttacct ggcatggtgt aaactacatc
     5581 gatggcgtct tggatcaccg ttttcttgtg gctcgctgtt tctcgattgg tatctacttc
     5641 tcctaacatt cctaacacat ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa
     5701 tagaggatca agcatccctc gacagctttc atctgaagat aacgaaataa aattaattcg
     5761 ttcatacata gccacaaact ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa
     5821 cttttgggtt tcatttttcg gatcaatata aagaattttt tgacctaaaa acatcagcca
     5881 taaaaaaata tattttacaa ggaccgattt tcctttacct ggtggaccaa taatcaacgt
     5941 atttccattg gtatgttccg cgccttcaat acttttcttt gttaaattgg gataatacca
     6001 tactagttta ctagatagcg ctaacgcttc tttcgcagat ttaaactttt ttgcaataac
     6061 aatccgtcct aaaggaaatc ctttcttatt tcctacttct ttctttaagt ccaagccata
     6121 atctgccaca aaacctgtcg tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc
     6181 aattaagcat tgattaaaca acgttagctg atccgctacc gatcgataaa atttgaaatc
     6241 tgtagactca atcgcaaatt caatatcttt gactcgttct tccaattctt cttttgaaga
     6301 agcagataca accagcgtga cggtagaata acacaatcgt ttttctttgc gcttcagctt
     6361 atcttttaca tccattaacc gttctgtacc aaaatttaat acatcgtctg aatctaaaat
     6421 gccgtcaacg gtctcatatt ctttttcttg ttcataaatc cgtccagcca ttttattaat
     6481 ttttcgttca tcagattttg aatgctcaaa tcgaagtcga atatgcgtat caattggaaa
     6541 agataagcta tcctgaagat tttgaataaa gctactccca tacatggcta aaggagcgtc
     6601 cgtttgactt aaaaacgtcg aataatgcgt ttgattcaac tgttcaatcg caatgtatcc
     6661 tgcttcattc gaaatgacgc cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa
     6721 tttttcttct gatcgatgga aaaaataata ataaatttgt ccaatctctt gggcagataa
     6781 gcgctctatc ggcttataat tggacaattc cgtatagagc tccttttcta atgtttcata
     6841 cgttggtaac acactactta taggaactcg ttgtcccgta atacgataga taaacttttt
     6901 aaaaacatgc ttaagcaatt gaaaatattc gatcggatca gacactttca tttctgtagt
     6961 gaaacgcaca aacagatacg ttcgatactc gtttaattgc acttcgtctt ttaaaatctt
     7021 tcctgcctgt tcaaagtaca ctttcccaag atctgcgcat tctcccttta ccaaatctcg
     7081 aatggtttct tcaatgtgcg tatctaggtt aaattgacgt ggaatattga taaaatgata
     7141 gtcatattca tcatgtccaa gtatgccttc gccatcttga agatagttct tgaaaaaatc
     7201 aatgtcattt aaaggaaata cttgggaaga aagttgatag cctacccaca catcatcatt
     7261 tttggtcaaa acaaaatttc ggataatggc gtctatcgga aattctaatc gaggaagctt
     7321 cgtttttttc tttttgaact gccaaatccc gataataaag acgaccaaaa gaaacaaaaa
     7381 cgccatttac tcgactcact ccttccattt gtgtagggaa tacgtcactt tttcatggaa
     7441 aaacggcaca tacatttcat gttcaaagcg ttgtttccgt ttctgataat aggacgttcg
     7501 cccaattaaa tactcgaaaa agtgtttatt gtcccatttc attgaaaaca atgtccaaac
     7561 gagaacacca atcccaaaaa tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa
     7621 actaatttga ttcacatacg caatcaaaaa taggatcaaa agaatgaaga gaaatacccc
     7681 acctatgact aattgttcca tgcgaatgcc attagaaatc cagggaattt tttgtccttt
     7741 aatcccgtaa attttgtgag ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac
     7801 tctacctcct tttacaaaaa aagaagcgac cagaagttct aatcttctga tcgcttttgc
     7861 agtttgtctt tttcttatga taattgtatc cagggcacaa tcatttgcgt tacccccacg
     7921 ttctcttcac tatccttaat aaagataatg ttctttagcc attcaccaat atcaacggca
     7981 ttcgataaca ataagacgag cattatcaaa ataaagacgc caataatggc aaacgctgcc
     8041 cgcgcaaagc cttgggtcgc ataagcacca accattagaa caatcgcaac gattgccaat
     8101 agccaaatga cttgtccaga tacatcatca aataacgatt taagatcaaa aagtttcatt
     8161 tccattctcc tctaagttca ttttattcat ttaacgtagt aacaaaataa ctatcgccac
     8221 ttgtttttac ttccattgaa aagtttgatt ggaactcgtt tcctttctca aaatagaaac
     8281 gatacttccc ttgcaaaaag tagtttcctt ctttattttt tactgcatga tccagttgta
     8341 cattgatcaa ttcgccgcct tctaaccctt tttcgttaga aatcaatgcc aatttttcat
     8401 catttttgac atataactca aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc
     8461 tttcagtgac cgcttgccca gaagaataaa atcgattttc ctgatagttc acagaattgg
     8521 tattcttact ctcagaaaga ttcaaaaatt gaacgccatc aagtactttt aaaacattgt
     8581 tttgataact gactggaaaa gaaagttgac tgcttttcca atgttctcct tctgtaaaag
     8641 taacctcata aaataggcga tacattgctt gtgtcaaatc gatttcttct agtttaataa
     8701 agcgaacatc tttcgctact cgattggtag ccgtcgttac aaagcctaga tcagacgctt
     8761 ccaatccttg tgctaaataa tgattgacgg aagcctccca ttcgtttcgt ttttctggcg
     8821 aataagtaaa taacctttca ataaaatgtt gtccctcgta cttcaattgt tccgtagaag
     8881 acaaggtttg gttgacttca gttaacaatg tttctggatt gacttgtttt ttcactgcta
     8941 aacgcgttaa tgtatccact cgtccaaagt ttaggaggag cacgacaccc attaacagaa
     9001 acaaacaaaa aaaagcgcct gaaaatacac tcatggcact tttttgcgaa aattggatca
     9061 ttcgatctga cggttctgtt ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt
     9121 ccttctgttg tttcggtggc ttagtttgga acgcttgtgg attaaatttt gcattactca
     9181 tgagaaatgc cataccgttc taacaataat ttcggtattt tgtccacaat cactacttcg
     9241 tggacaacca cacttgcccc ctc
//
LOCUS       DAJSBM010000087         9213 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJSBM010000087 DAJSBM010000000
VERSION     DAJSBM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.090"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            4..2376
                     /locus_tag="ONT67_002472"
     CDS             4..2376
                     /locus_tag="ONT67_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356188.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ONT67_002473"
     CDS             2437..3897
                     /locus_tag="ONT67_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR2356189.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /locus_tag="ONT67_002474"
     CDS             3908..6112
                     /locus_tag="ONT67_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCR2356190.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            6133..6282
                     /locus_tag="ONT67_002475"
     CDS             6133..6282
                     /locus_tag="ONT67_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356191.1"
                     /translation="MRNTTHKEQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     LELDR"
     gene            6358..6951
                     /locus_tag="ONT67_002476"
     CDS             6358..6951
                     /locus_tag="ONT67_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCR2356192.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            6967..7866
                     /locus_tag="ONT67_002477"
     CDS             6967..7866
                     /locus_tag="ONT67_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCR2356193.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="ONT67_002478"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="ONT67_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2356194.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="ONT67_002479"
     CDS             8720..8980
                     /locus_tag="ONT67_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356195.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         3464 a         1547 c         1926 g         2276 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga caatcgaaaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc agaaaaagga aaagaaaatc ttcttcctga agtacaagca
      481 attattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 gactatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctttgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgaa
      961 aaggaacaaa aacaaacggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taagcaagaa
     1081 gtcaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtgaaag aacttttgaa ttttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc tgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacggggg gcatatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac ttcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccacagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 tcattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac taaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggattg gaatgatcaa ttgaagcatg aaatctacca acaagagatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggtagac agcaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc gattgttttc
     4021 aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agagcatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatagcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagcccg ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 gaaatatcta agctacccac gaacagatac cccttatatt acagaagggg agtatgcgta
     4921 tctgttggat cacttagacg aatacaaaca ctttttaaag gccgaagcca ttcctactcc
     4981 tattcataca ccgaatagtc gatatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aagacggtca tgacggcagc ggcttttgaa caattaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agcgacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgt taacaagtgc cgaaatgacc gccaaatggg aaagctatct
     5641 tacaaagatt ggtgagcaaa aagggacaca agctactttt ctagcaaata tccaaaaatt
     5701 tgtttcccac ttgttggaag tcgtcccagg acaaatccaa gccactgatt ttggagcaac
     5761 gttacaagag gtgaaagtag ccagtgaaaa gcaagaagcg gcgcgtcatt taggatcttg
     5821 tccaaagtgc caggaaaaag aagtcatact ttatcagaag gtggcgactt gcacgtctga
     5881 agcctgtgat tttaaattgt gggtaacgat tgccaaaaag aaactcacag cgacacaact
     5941 gaaagagatc attcagaacg gccgaacaag tcaaccagtc agaggactaa aaggtcagaa
     6001 aggttcgttt gaagcaacga tcgttttaaa agaagacttt acaacaggat ttgagttttc
     6061 tgaaaagaag aagaccacct acaaaagaaa agtgagacgt aaaacgagtt agaattgacg
     6121 gagggaaaaa cgatgagaaa tacaacgcat aaagaacaag aaatcgaaaa tcaaaaagcc
     6181 aaaatacgtt cagagcagga gaaaatgcgt gccagcaaac gaagactaca gcaattgaag
     6241 aatcaacaac ggattcaaaa agaacttgaa ttagatcgat aaatcctcct gttgttaata
     6301 ttaaactaac atgttatagt tatgttagtt taatattaac attcgagagg gaaagcgatg
     6361 aaagataaag tcaagtgcat ttttgagaaa tacaatggta atctatcctt gaaggatgct
     6421 agaaatgaag ggttatcacc tatgacgtta gatcgtttag ccgaaaagga caaaatagaa
     6481 aaaatagcat caggttttta taccttaaaa gatagttttg tggatgaatt atatctcgct
     6541 caaagtatct atactagagg aatcttctct cacgagacag cgcttgatct ttttcaagta
     6601 ggaacatata tccctaaaaa aattaatatg atgtttccta aaggatacaa tgtttcgaaa
     6661 gagaatttag atcagtatgc cattcaaccg cattacacga ataaagaaaa ctttgaactg
     6721 ggaatcacag aggcagaaag cttttacggc aataaaattt tcgtctatga tttagagcga
     6781 acgttatgtg atatgtggaa tcccagatat aaagcaagcg tagaagtaaa acaagaagca
     6841 ttaaaagaat atatgacaag taccagcaga aatacaaata agctaagaag atacatgaat
     6901 attttaaaat caccaaaaga aatgactttg tatatgttgc cactttattg agaggagtaa
     6961 aggtgaatga caccgacaca atttaaagcg aaaacaagaa atgttgcagc tgagaaacat
     7021 ttaaacccac aaattgttca aagatattat atgatggaaa aattattaga atgtattgtt
     7081 gaatcgcctt atcatgatga ttttgtatta aaagggggat ttctgatcgg atcaaaatac
     7141 gggttagaaa accgttcaac cgttgatatt gatacgactt atcgaaattc gaaactaaca
     7201 aaagaaaaat tagaaactgt tttttctgaa ttaacagctg ctcctacaaa agatggcatt
     7261 cggtttgaat taaagggctt aacagagacg agggaagccg attattaccc aggttttcaa
     7321 gcaaagttta ttgcttttct tgaaaatgca cgtgtacctt ttaaattaga tattacttca
     7381 ggtgattcta ttattccagc agcattgata tatgaacata aattgatgtt tgaagataaa
     7441 acaattcata ttccagctta tcctacggaa caaattttag ctgaaaaatt acatgccacg
     7501 tttagtttcg gaatagataa ttcacgtatg aaagatttct atgatctgta tatgatccct
     7561 aaactagaac gtatcgatga gataaatttg tataagagtg ttcaaggaac atttaaggaa
     7621 agaaataatc aatcagtgtt taagaatttg tataatcagg aaatgcctca attgatgaag
     7681 aatgagggat tgagggagaa atggaaaaga tatcagcaag aaaactattt tgccaaagat
     7741 atttctttcg aatctacctt aacttctgtt gattcgctga tgcaatcaat tgtcgagcaa
     7801 gaggaagtag aacgaaaaca aaattttaag agatcacaac aaatgaatca agaaatggag
     7861 agataaagat gatcaataat gtaactttag taggtcgatt aaccaaagat cccgatttac
     7921 gttacaccgc aagtggcact gcagtgacca cttttacctt agccgttaac cgtaatttta
     7981 ctaatcaaag tggcaatcga gaagctgact ttatcaattg tgtgatttgg cgcaaaccag
     8041 ctgaaacgat agcgacatta gctaaaaaag gcagcttgat cggcgtcgtc ggacggattc
     8101 aaacgcggac ttatgacaac cagcaaggac agcgtgtcta tgtgactgaa gttgtggccg
     8161 ataatttcca actattagaa agtaaagcag cgacagaaag ccgtatgcac gctgatcaat
     8221 cgtccagcag tccaagccct accacctttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ctaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagacaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggc
     8461 tttcttttac gtttctatta taagaattaa cgggtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cgcgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac aggaaaagac ctttttagga
     9061 agacacaagt gccttctcaa ttgaaaaacc tttctctcac tccaaccgcc accgccgttc
     9121 gagaagaaag gaagtgaacg ctgcacgttc actaagatat ggtgtcttgc aaagcaagac
     9181 aaaatagaca aaacttcctc tcaccaaaag gtt
//
LOCUS       DAJSBM010000088         9195 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJSBM010000088 DAJSBM010000000
VERSION     DAJSBM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.091"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            136..309
                     /locus_tag="ONT67_002480"
     CDS             136..309
                     /locus_tag="ONT67_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356196.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="ONT67_002481"
     CDS             949..1422
                     /locus_tag="ONT67_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCR2356197.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="ONT67_002482"
     CDS             1431..1658
                     /locus_tag="ONT67_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356198.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="ONT67_002483"
     CDS             2294..2665
                     /locus_tag="ONT67_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356199.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="ONT67_002484"
     CDS             complement(2921..3163)
                     /locus_tag="ONT67_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2356200.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="ONT67_002485"
     CDS             complement(3195..4097)
                     /locus_tag="ONT67_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2356201.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="ONT67_002486"
     CDS             complement(4110..4298)
                     /locus_tag="ONT67_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2356202.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ONT67_002487"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="ONT67_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2356203.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="ONT67_002488"
     CDS             complement(4913..5806)
                     /locus_tag="ONT67_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2356204.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6806)
                     /locus_tag="ONT67_002489"
                     /pseudo
     CDS             complement(5884..6806)
                     /locus_tag="ONT67_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6855..7205)
                     /locus_tag="ONT67_002490"
     CDS             complement(6855..7205)
                     /locus_tag="ONT67_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2356205.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="ONT67_002491"
     CDS             complement(7238..8140)
                     /locus_tag="ONT67_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2356206.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="ONT67_002492"
     CDS             complement(8133..8990)
                     /locus_tag="ONT67_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCR2356207.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2582 a         1743 c         1510 g         3360 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccac
//
LOCUS       DAJSBM010000089         9048 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJSBM010000089 DAJSBM010000000
VERSION     DAJSBM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.092"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="ONT67_002493"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="ONT67_002493"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR2356208.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="ONT67_002494"
     CDS             2714..2938
                     /locus_tag="ONT67_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356209.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="ONT67_002495"
     CDS             2951..3451
                     /locus_tag="ONT67_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCR2356210.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="ONT67_002496"
     CDS             3548..5395
                     /locus_tag="ONT67_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HCR2356211.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="ONT67_002497"
     CDS             5398..6294
                     /locus_tag="ONT67_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356212.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="ONT67_002498"
     CDS             6344..6733
                     /locus_tag="ONT67_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2356213.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9048
                     /locus_tag="ONT67_002499"
     CDS             6720..>9048
                     /locus_tag="ONT67_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356214.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         3196 a         1362 c         1909 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaacg
//
LOCUS       DAJSBM010000090         8921 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJSBM010000090 DAJSBM010000000
VERSION     DAJSBM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.093"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="ONT67_002500"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="ONT67_002500"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2356215.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1713..3059)
                     /gene="gor"
                     /locus_tag="ONT67_002501"
     CDS             complement(1713..3059)
                     /gene="gor"
                     /locus_tag="ONT67_002501"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR2356216.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3193..4446)
                     /locus_tag="ONT67_002502"
     CDS             complement(3193..4446)
                     /locus_tag="ONT67_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2356217.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4452..5375)
                     /locus_tag="ONT67_002503"
     CDS             complement(4452..5375)
                     /locus_tag="ONT67_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR2356218.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5596..8088)
                     /locus_tag="ONT67_002504"
     CDS             complement(5596..8088)
                     /locus_tag="ONT67_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCR2356219.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8095..8562)
                     /locus_tag="ONT67_002505"
     CDS             complement(8095..8562)
                     /locus_tag="ONT67_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCR2356220.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8698..8771)
                     /locus_tag="ONT67_002506"
     tRNA            complement(8698..8771)
                     /locus_tag="ONT67_002506"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8735..8737),aa:Arg,seq:acg)
     gene            complement(8829..8902)
                     /locus_tag="ONT67_002507"
     tRNA            complement(8829..8902)
                     /locus_tag="ONT67_002507"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8866..8868),aa:Pro,seq:tgg)
BASE COUNT         2479 a         1884 c         1485 g         3073 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg
     1081 aaaatggctt tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa
     1141 caccgtttat cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg
     1201 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa
     1261 cactatttgt tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt
     1321 cacttgattt gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat
     1381 ggtattcttt taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt
     1441 ttaaacatct gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga
     1501 acttaagagt gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa
     1561 atctccaact tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa
     1621 ctaaaaaaca aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc
     1681 aataaaaagc tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct
     1741 cctgttggat ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata
     1801 gccacggcaa aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg
     1861 attctttctt cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt
     1921 aatgcaaagt acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat
     1981 ttttccaatg cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg
     2041 actgtcggaa caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct
     2101 gataaacgtc taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca
     2161 ccaacagcaa aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct
     2221 tttttgtcca attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt
     2281 ccaccggcaa aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta
     2341 tattcgtcat tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca
     2401 gcttcttggt acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt
     2461 cgttcttttc gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt
     2521 tcagccgcaa tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca
     2581 aaaaatccat ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg
     2641 cctccagtag cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt
     2701 tgactatcga taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct
     2761 ctttgataag caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg
     2821 aaatcaacat tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt
     2881 tccaaaatcg tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat
     2941 gttccgccca attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg
     3001 tttgctgaag caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt
     3061 ttgttcctcc ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag
     3121 aaaacaagtg taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata
     3181 tcagcttttt ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac
     3241 tggcatagtt aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt
     3301 atgcataact tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc
     3361 atctcctaca gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata
     3421 agataatact tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg
     3481 ttcgttttca gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt
     3541 ccataactcg tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact
     3601 gtctacagcc gctttataga catttgacac cgtagataca taatggatcg atttcattct
     3661 tccttcgatc ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat
     3721 tgacatatcc actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact
     3781 gtttcgttct tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga
     3841 acatccgcca cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga
     3901 ataagagata cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg
     3961 aatctctgcc acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc
     4021 ttctttccag aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat
     4081 cggcagacct ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac
     4141 tgcagaaata ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc
     4201 ttcatgggtg accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc
     4261 aactccttca gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc
     4321 attcccgcca atatatactg catctgctcc ataatggatc gcggttttta acttttccag
     4381 tgttcctgct ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt
     4441 catcatactc cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca
     4501 cggttttcag gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc
     4561 cagctacctt ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat
     4621 ttttcgcctg gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt
     4681 tcattcaatt cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta
     4741 tcttcataaa tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt
     4801 tcacgatctt tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt
     4861 ttggattgat ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt
     4921 tcttccatcg ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg
     4981 ccttttttcg cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa
     5041 atatacggta tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca
     5101 actgtgatca catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc
     5161 attttttcag gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt
     5221 gctaattcta ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca
     5281 aattgctctt caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt
     5341 tcaatagttt ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata
     5401 atagtagcat agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa
     5461 aattatttca caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga
     5521 ttgtgacaaa aatcttgtca caacctctta atcaagtctt actattataa aaattcagct
     5581 acacaggcca agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa
     5641 tgtgatctta ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc
     5701 aagcaaagcc tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg
     5761 tgcaccatat tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat
     5821 tttcacttga atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac
     5881 gattgtatgg atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt
     5941 caagaattca ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt
     6001 gtgatcttta gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt
     6061 cgctccgata tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt
     6121 agaatctgtt aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg
     6181 tgctttctcc acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt
     6241 caactgtcca ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt
     6301 actgtatttt tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa
     6361 catgacttct gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa
     6421 catgaatgaa ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc
     6481 acgtgaaact gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc
     6541 cagttctaac aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca
     6601 ctccgataca actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg
     6661 ttttgcttca attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg
     6721 taattgagca gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag
     6781 ttcatcgatt tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg
     6841 gactttcgct gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt
     6901 gatataacga acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc
     6961 atggtgctct tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt
     7021 tggctcatct acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata
     7081 tttttgatat tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc
     7141 cggtttcaag atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt
     7201 atgcaattca tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt
     7261 tttcattcga tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc
     7321 aagcatcatc aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg
     7381 agctaaccct tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt
     7441 attctttgtc cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac
     7501 aactgggtcc agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag
     7561 cgtaggtgtt ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt
     7621 cattcctttt ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc
     7681 ttttgaaagg ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt
     7741 taataagctc atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc
     7801 gctgctagct aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt
     7861 agggtaagaa cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt
     7921 ttcattcaaa tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa
     7981 ggctagtaaa acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg
     8041 tgcaatttct aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt
     8101 cccatcttca taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc
     8161 ttcatattca ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc
     8221 tttttttgta attatttggt cttcataaag tttttgaata atagcaaaag catttttctc
     8281 actgattgta tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat
     8341 ccttactttt gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc
     8401 acgttggatg gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa
     8461 gaggtctgcc atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt
     8521 tatatatgat tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga
     8581 ctaatattga ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt
     8641 taaataaatc ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatcgacgc
     8701 gcccaagagg agtcgaaccc ctaacctttt gatccgtagt caaacactct atccagttga
     8761 gctatgggcg catattcaat ttaaaaaaat tataaatcat ttgaaaacaa aaagcaagat
     8821 aaaaaaaatc gggaagacag gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc
     8881 taccaagctg agctacttcc cgtttaaaaa ataaaaaaga c
//
LOCUS       DAJSBM010000091         8740 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJSBM010000091 DAJSBM010000000
VERSION     DAJSBM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.094"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..83
                     /locus_tag="ONT67_002508"
     CDS             <1..83
                     /locus_tag="ONT67_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCR2356221.1"
                     /translation="SVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            74..859
                     /locus_tag="ONT67_002509"
     CDS             74..859
                     /locus_tag="ONT67_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCR2356222.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            828..1544
                     /locus_tag="ONT67_002510"
     CDS             828..1544
                     /locus_tag="ONT67_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR2356223.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(1646..2680)
                     /locus_tag="ONT67_002511"
     CDS             complement(1646..2680)
                     /locus_tag="ONT67_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCR2356224.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2684..3742)
                     /locus_tag="ONT67_002512"
     CDS             complement(2684..3742)
                     /locus_tag="ONT67_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCR2356225.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT67_002513"
     CDS             complement(3962..5110)
                     /gene="nagA"
                     /locus_tag="ONT67_002513"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR2356226.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(5186..6022)
                     /locus_tag="ONT67_002514"
     CDS             complement(5186..6022)
                     /locus_tag="ONT67_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356227.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(6206..7423)
                     /locus_tag="ONT67_002515"
     CDS             complement(6206..7423)
                     /locus_tag="ONT67_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCR2356228.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(7572..7751)
                     /locus_tag="ONT67_002516"
     CDS             complement(7572..7751)
                     /locus_tag="ONT67_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356229.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            7806..7889
                     /locus_tag="ONT67_002517"
     tRNA            7806..7889
                     /locus_tag="ONT67_002517"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7840..7842,aa:Leu,seq:gag)
     gene            complement(8129..8707)
                     /locus_tag="ONT67_002518"
     CDS             complement(8129..8707)
                     /locus_tag="ONT67_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356230.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         2765 a         1775 c         1466 g         2734 t
ORIGIN      
        1 aatcagtgat aatctatatg acgttttgtt taaagagacg attgagcatc aaacgaccgt
       61 tcggactcaa tccatgtatt taaatgggat agaaactatt tctagcgtta cacctctttt
      121 tagcaatgac cgttttttag gagcagtcgc aactttacga aatcgatcag aagtaatgaa
      181 attaatggag gagcttgggg ggacgaaaca atatataaac tccatgagag ctcaaaccca
      241 tgaattcatg aataaacttc atgtcatttc tggtttgatt gatttgaaga agtatgaaga
      301 agtcacccat tttatccagc agctaaatca aaattataaa aaagagattg gccaagtctc
      361 aagtctgatc aaagtacctg caattgccgg ttttttatta ggcaaaataa acgaagccaa
      421 agaacaatct gttgaaatgc tgttagaaag cgattctttt atccctaaaa tggagatgga
      481 tgactccatc cattcacttt tgcaagtgtt agggaactta cttgataatg caaaagaagc
      541 cgtgctacaa aaacatgaaa gaaacggaca ggtccggatt catttaaact atgacacaga
      601 agctgaagta ttcatcgtga ccgtctgcga tggcggtgtc gggatatcag aagaaatctc
      661 agaaaaaata tttacgctag gtttttcgac gaagggaatc aatcgtgggt ttggattgaa
      721 tctcatccat tcaatcgtca aacgccacgg tggattttta gaattttcca aacaaacaaa
      781 tggcggtaca atcgtctatc tagaattacc aaaaaaggaa gatcaaaatg atgacaaatg
      841 tcctgataat cgaagatgat ccaatggttg cagaattgaa tcgtactttt gtggaaagca
      901 tccctaatat gaacgtaatc gaaagtttca gaaatgtaga agaaggaaaa aaatatttca
      961 gacaaaaaaa cgcagatctg ctgctgcttg atgtatacat gcctggcgaa agtggtatcc
     1021 attttttatc ttggcttaga cagcaaggaa atgatatccc agtgattttg atcactgctg
     1081 cagatgatat cgaaaccata aagaatgtga tccgctacgg tgccttagat tatttgatca
     1141 aaccgttcac tttgcaacgt tttcagctgg cgatcgaaaa gtatcttgct ttctctcaga
     1201 caatccaaac gactgaaaaa gccgatcaga aattgatcga tcaagtattc aaccacacca
     1261 ctcccattca cgaaatacca tcattcatcg atttgcctaa aggtctgtca aaactgactt
     1321 taaaaaaaat aagtgcattt attctaaaag aagatactat tttttcaaca aaagaactgg
     1381 ctcaaaaaag ctcactgtca cgtatatccg tcaagaaata tttgaatttt ttagtagaac
     1441 aagatatatt gaacgaaaga atcaaatatt tggaaatcgg ccagccgcta acctgttatg
     1501 aattgacaaa gaatgggcgg aaaaagctca cagattactg ttgagtcatc gaattatccc
     1561 atacaaaata gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat
     1621 tttattgacg aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct
     1681 catttttcgt ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca
     1741 tatttgataa tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt
     1801 acatttctac taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc
     1861 ggaaaaaaac ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc
     1921 ttttcaaagc tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca
     1981 agatgctacc ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac
     2041 ctgggatatg aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg
     2101 agtggaacgt caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt
     2161 tggctcctag tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga
     2221 tccctactgt gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt
     2281 tgaaagatgt tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca
     2341 aagataactc tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa
     2401 aatcagctgc cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac
     2461 gaatcccata ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca
     2521 acaatacagc atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat
     2581 aaagactgag atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac
     2641 gaacaccgta acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta
     2701 tgccatgaat catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag
     2761 ttgacagtaa ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg
     2821 ctgctgccac tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa
     2881 aattgactgg tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag
     2941 gtacaaatac cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt
     3001 tgaagcttac ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac
     3061 cagctaaata atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg
     3121 ctacttggct tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga
     3181 acagccaaat gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt
     3241 tgatattttt agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa
     3301 agattcggta gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg
     3361 cactgacgat aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg
     3421 ttgtttgctt atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa
     3481 tataaataac taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga
     3541 tatcttgcat gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg
     3601 atagaatttt tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata
     3661 atataataaa aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc
     3721 gaaaaatgtc aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa
     3781 aaaagctttt attttgctaa tacgtattaa catttttaat tttatcttta cattgttact
     3841 tattgaacaa taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga
     3901 acacaaaaaa accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat
     3961 attatgcgtg gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt
     4021 tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt
     4081 tacagctgat tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc
     4141 cccagtctac aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc
     4201 cagattctag acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat
     4261 ttccatcagg catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc
     4321 ctgctttttc cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca
     4381 attcagaaaa gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat
     4441 tcaatccacg cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt
     4501 tttgcgcttc ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag
     4561 tcacttgttt gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga
     4621 ttaaaccacc tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat
     4681 aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc
     4741 ctttgatttt agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt
     4801 ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata
     4861 acagaccttc agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca
     4921 tatagccgtg aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa
     4981 tgatgttgac ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa
     5041 tttcaagata gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa
     5101 aagttctcat tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga
     5161 agaagtcaag aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg
     5221 ctaagccttc atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt
     5281 taagcacaaa taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc
     5341 cttgattcac ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat
     5401 aattataaaa aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca
     5461 gttggatgct tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata
     5521 tttttccgat acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt
     5581 ggctagagta gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca
     5641 acgtgaattc gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca
     5701 gattttcaga aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt
     5761 ggaaacaact atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga
     5821 gattcatcac atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata
     5881 cattcggcag ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat
     5941 gcgaaagcat tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa
     6001 aggactcgat taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt
     6061 cgtattttac tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact
     6121 ttagttattt aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc
     6181 ctactgaaaa atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca
     6241 ttttcttagc tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat
     6301 ctggctggtt ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa
     6361 agtccaaaac gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga
     6421 atatataaaa agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat
     6481 tatagatata ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac
     6541 tttgataacc gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac
     6601 gcatggagga caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac
     6661 ttaggaccat ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca
     6721 gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca
     6781 tcactaagcg atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt
     6841 caacgatttc ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa
     6901 tgataacaat agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat
     6961 accattctgc ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag
     7021 aagccgtcca caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca
     7081 acccctgcat acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga
     7141 tcgtcacatt cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa
     7201 agatcccctt ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa
     7261 tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa
     7321 tactaatgcc ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt
     7381 aactgacacc ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc
     7441 atttcaaaaa gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc
     7501 atcctagaaa attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa
     7561 gcgttgagaa cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca
     7621 attccacaaa tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc
     7681 aatacaccta agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata
     7741 agtcctccca tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt
     7801 attacgcaga tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc
     7861 tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg
     7921 agtttttttg tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa
     7981 aaagaacgta caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta
     8041 ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct
     8101 aatactaaag cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac
     8161 aaataccaag cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt
     8221 tcattagctc tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt
     8281 acgtccgcat tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa
     8341 atcccattat catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat
     8401 aaattccaac aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac
     8461 ctggataaaa aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac
     8521 tactccaaat cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat
     8581 taccaaaata aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat
     8641 agttttgaca tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga
     8701 tattcatgtc taaaaaaaac ggctttccaa atgatgaaat
//
LOCUS       DAJSBM010000092         8489 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJSBM010000092 DAJSBM010000000
VERSION     DAJSBM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.095"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..173)
                     /locus_tag="ONT67_002519"
     CDS             complement(<1..173)
                     /locus_tag="ONT67_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356231.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="ONT67_002520"
     CDS             complement(593..1714)
                     /locus_tag="ONT67_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR2356232.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ONT67_002521"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ONT67_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR2356233.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ONT67_002522"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ONT67_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCR2356234.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ONT67_002523"
     CDS             complement(4304..5215)
                     /locus_tag="ONT67_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCR2356235.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ONT67_002524"
     CDS             complement(5232..6239)
                     /locus_tag="ONT67_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR2356236.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ONT67_002525"
     CDS             complement(6236..8365)
                     /locus_tag="ONT67_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR2356237.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8489)
                     /locus_tag="ONT67_002526"
     CDS             complement(8370..>8489)
                     /locus_tag="ONT67_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356238.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1485 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgacc
//
LOCUS       DAJSBM010000093         8330 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJSBM010000093 DAJSBM010000000
VERSION     DAJSBM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.096"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(63..1022)
                     /locus_tag="ONT67_002527"
     CDS             complement(63..1022)
                     /locus_tag="ONT67_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2356239.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1118..1663
                     /locus_tag="ONT67_002528"
                     /pseudo
     CDS             1118..1663
                     /locus_tag="ONT67_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1846..4521
                     /locus_tag="ONT67_002529"
     CDS             1846..4521
                     /locus_tag="ONT67_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCR2356240.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4703..4942
                     /locus_tag="ONT67_002530"
     CDS             4703..4942
                     /locus_tag="ONT67_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356241.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4958..6331
                     /locus_tag="ONT67_002531"
     CDS             4958..6331
                     /locus_tag="ONT67_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356242.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            6335..7969
                     /locus_tag="ONT67_002532"
     CDS             6335..7969
                     /locus_tag="ONT67_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356243.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8132..>8330
                     /locus_tag="ONT67_002533"
     CDS             8132..>8330
                     /locus_tag="ONT67_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356244.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2876 a         1338 c         1644 g         2472 t
ORIGIN      
        1 aatgaaaatc acctttataa actccattct tttccactgt tttattaata ttttctgatt
       61 tgtcaaatta agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt
      121 gttcgataat ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata
      181 aaggtttcat ccgtgtccct aaagtccatt gttttaggta agccatctcg tctaagcaag
      241 ccgtttgagt gctcatttag ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg
      301 gcaatatcgt tcgagttact tatagatcgc cagttggaaa attcttttcc acaatcaaat
      361 gtcatggatt taaacaagtg tttaggcatg gatttgaacc aatcatttaa cctgttttcg
      421 atatcttcgg ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga taaacactca
      481 accaatgtaa taacagcgct cttgtgttta actcccacaa tggtgtcgac ttcgagatga
      541 ccaaattctt tagtcaggtg tggatagaga ctgttccgtt catgaatcgt tctgcgaaag
      601 gcctgttttc cacgcttttc ttgatgatta ttcggtttac gtttcccctt cataggtaac
      661 gactgtgtgt caaaaaggcc ttttttaaac atccgataga gtgtacgaac agaacaatca
      721 attggaaact cgccacggcc gataatcaca tcgggtgacc atccttgagc taccttttct
      781 tgaacatatg ttgattgttc atcagataaa acggttgaac gtcgtccaca acgagattta
      841 ttttgtttgt attgacggta ataatctagc actttttttc cttgtttgag aaacgaataa
      901 acattataaa tggtttgtct agagcgattt aaactttgtg cgacaagagc gacaggtttc
      961 tcgatattga aataagactc tatgattact agttcgtctg ttgtaagatg ggtgtaagtc
     1021 atttgtactc actccttatt aattctttgg tcggaactat tttgagtgta gcacaaatga
     1081 tctttttagt tgtctagctt aattttacaa tcgacgtttt caaaatgctg agactattga
     1141 acgagaacat cattatttcg atttattagt gaatacagtg gtagaattag cgaaatgggc
     1201 aaagtcaaac gaaacttata gtcaagtact taaaagaatt atgagtcaag aaacttcatt
     1261 tatctatgag ttaagcaagt caaatttgaa atatacttat aatgaagatg aacttgaaat
     1321 tttagataat ccagaaaatt cacaatggat taaagagttg aagagagaag actttattga
     1381 gacactaaga attctaaaat cgttatttga tttgaaatta gccaaagcta ccatcatggg
     1441 aattgaaaga gattcatatt attgggagga aattggacta tatgctcccc agattgaagg
     1501 tgaagataaa gagaaaagga tagacaacat tattaactat attaatagtt taagtgaacc
     1561 tttaaaatgt agaggacttg attttatagg aacattagga tggacagaaa catactatga
     1621 gaaaatatat aaagaaataa ttgaacaata ttttcctaaa taatttaata aggattgatt
     1681 tatcgtaaaa gaaaaaagtt gtgatttcca aaatcacaac ttttttcttt tgtcaagatt
     1741 ttgagtggga tatatttttg tagaactgaa gatcatgaaa ttatgagagg ttggaggtaa
     1801 tgtagtcata caaataaaaa ttttttagaa agtgagtggt aattaatgaa aatgaatcac
     1861 acttcaattc agaagcattt acaactgtta aatacactgg ggatttctta tgttaatttg
     1921 agaacttttt ctacattaaa caagaaaacc gtttctcata ttttttctac tgcagattct
     1981 caagcaatta ttaactattt agaaaaacag aaattgtctg aaggtacagt aaacataact
     2041 tataatccta ttaaacaaga ggtgattaca caaaaaaagc attctgtgag agacaatgat
     2101 attgaaaaaa ttcggtttgt atttattgat attgacccta aaaggaaaga gggaagtgca
     2161 acagatagcg aaaagaaaaa ggcagaaaat gttatggaac aagtagaacg ttatttgaaa
     2221 gaaaagagaa tagaatcatt tgtaaaagtt gatagtggga atggctacca tatttttctt
     2281 cccataaatg aacaaccaaa taatcacgag acgattctta ctattcagaa ttttcttcaa
     2341 ttacttcacc gaagatttgg agtagaagat gaagttgata ttgatacttc tgtttataat
     2401 ccttcaagat tatgcaaatt agttggtaca ccagcggtca aaggaaagtc cacaaaagag
     2461 cgtccgcatc gtttatctag tttcctttct attcccgata acatagatag aagtaattgt
     2521 tttaaaattc ttgaacagat agttgaagaa aattccatga tgtcaacctc tagagtaaaa
     2581 agccatccat cttttagaga agtgttacct tttattcaag cagactataa acaatggtta
     2641 gctcactatc ctgaattaac ttataccata aaagagggcg atcataaagg agttacctta
     2701 cttatatttg acgaatgtcc attaagaaaa cacacgaata attctaatgg ttcttgtttg
     2761 tctgttacgg ctgataataa gattcgattt cattgtctgc atgcttcgga taaaaataaa
     2821 gatattcaag actttgtaaa aaaatatccg ttgccaaaac ctgtaaatcc ttctgtagaa
     2881 tcattagaat ccaatcaaga atattatttt gaccagttta aactgaatca agaaggagta
     2941 tttttggtga aagaaggtca agaagaaaaa gttgcttgtc cgatgtttat taagcaaata
     3001 cgcaaaaata tcgaacaaaa agtcattaca cttgttcttg cctattatgt cgatcattgt
     3061 tgggatgaaa tagaaataga aggtggatgt ttacaaacta ataattttaa acaattagca
     3121 aaacatggct taatcattaa agctagaaga gaagctgaag tcgttgactt tttacagatt
     3181 caacgggaga atgctccaat cttttttgaa catgaatctc ttggctggaa aaaagaaaat
     3241 gaagcaatga tttttcagtt acaagattct tttggacgtg aaattccttc tgtattaact
     3301 aaaaactcat gctaccaatt aacaactaat ggtacagaag cggaatttga cgcattggtt
     3361 cataagtatg ttttaggaac gaacttagaa cttgcttggt cgattggtat tgcttctatt
     3421 gtgttaggct atttaaacgt tacgaaggct aatacttttt tgagtttact aattaaccta
     3481 cagggcaaga gcacaacggg taaaaccact acgttacact ttattgcctc tttatttgga
     3541 aatccaacta cggttcttag aaatatgaat gctaccaata aagccattat aaagctagca
     3601 tcaaacaatc agggaggtgt tccactaata ctagatgagt taggagcagc aactcaaaat
     3661 aactttagct cgttgatcta tcaattagct tcaggagaag aaaggctaag attagattct
     3721 aattgtcagc ttatgccaca agagactttt tctgttttga cccttacctc ttctgaagag
     3781 cgtttggaaa attatttcaa tgagaaatta actggtttga aagttcgata tatcgaattt
     3841 tctgatatca agtttacaaa ggatgcgaat cacgcagaga aagttaaaag tctttcttct
     3901 aatacttttg gttggggtat aaagttattt cttttgaaac tctttgaaca agggattaca
     3961 aaaattgaag caacttttgt cgaagctcaa aaaaaactat gggctgtttt acctgaacat
     4021 tctttgaagc aacgcattgg taataatcta gctttgattt atagtggagc cctcctaatt
     4081 caagatatac tgggctatcc tttacgatta gagcatatac agacggtgct tattaattcg
     4141 tacgaacagt taattgtaga tactagttta ggaagtgagc aaacggtatt tgaccgagtg
     4201 aaaacattgt tggtgatgaa tagccataaa tttttaacaa acagtgacaa caaacatacg
     4261 cccagtacga tttggggaaa agtatcaatt caaaaaaatg gggatattaa ggtaaatatt
     4321 tttcctatcc cgtttgagga cttgttaagg cgagaatttc aagtaaagga tctaacctat
     4381 ctttttaggc aattgcttga ttcgggtaat atgtgtgcag aaaaagggag aagaacgaaa
     4441 cgtattcaca tgaatcgtca agttcttcca acatacgaga ttctattacc ttcttcactc
     4501 aaatcgtatt ttcgattgta atttagaata aaccaaagta tgaaagtgag gcaccatgtt
     4561 atggaaatat ttcttattga tttagcaaaa caactgatta cttatctgta tgatagcgag
     4621 caaacccaaa cggaagtaaa tgaagcttga tctatgcttc atttacttca ttcaatcaaa
     4681 catgaaagaa aggaaaattc taatgactaa aaataaaatg acactaaaag cagaggttct
     4741 tctgtatatt caagaacatt tttcaaatca agcctttttt acaaaaccaa tttacttgga
     4801 tttcgaaata agaggggtat ctgctggatc tatcgggggt actcttcaag cgttgaagaa
     4861 cgaaggatat ttagaaaatc actttgtgca acgttcgttc aatagaagag tcgtgaaaaa
     4921 atggtattta gttcattcat agagagggga atcaagcatg cgtgtggctt gttatgatcg
     4981 aacgtctgtt tatgaagaag cgaaaattag taatgacgct caatatgatg cagtgaaaga
     5041 gtatgtttta gagaaatatg aacaaattcc ccaacgattt tcagacaaag gagaatcagg
     5101 ggaaaaagcg gagcgtccag cttttcgaca gcttagagct tgggtttgtg cgtcagaaga
     5161 agagggggtg atctatgttt ggcgttatga tcgattggca cgtcaagcca aaatagcctt
     5221 agaatttatt gagttgtgcc aacaacatca ggttcaagtg tattcgattt ctgaacctct
     5281 tcctgcttct ttctcacacg cgtcaacgaa taaactattt gtgcaattat tgttcacttt
     5341 tgcagaacaa caacgaaacg tcattattga gaatgttcgt gccggacttt cctataaaaa
     5401 gcaacaacgg caatatttgt cttctgcggt accatatggg tatcgtttag tgaatgggaa
     5461 aatccaacaa gaagtacacg aagcaaggat tgttcgctat atttttcaac tttatttgac
     5521 caaaaaatat ggctacaaaa agctttgtca acgattgacg caacaaaagc ttttcttccg
     5581 cgaacgtcct tttcagccct atcatattta tagtatccta aaaaatccat tgtactatgg
     5641 cgaaatcaaa ggaggatctt tggggaagta tctaggaacc tttgaaccga ttctctcgaa
     5701 agcaaccttt ttacaagccc aagagattcg ccaatcaagg tgtacagcta aaaaagatac
     5761 gtatctgtac ttattacgtc aaaaaattcg gtgtcccttc tgtggtcgtc atctgagttc
     5821 gaagtatcaa tggaatacaa agaagacgaa gacgcttcat tattatcatt gtaccgaccg
     5881 aagctgccaa ggaatttttc ttcgggcaga aagggttgag tcagaagtat tggaaaagat
     5941 acaggctttt tttcaatacg aaggcgtcta tcaagccttg attgcccaat taacgcaaca
     6001 attagccgaa aagcgaaaag atgaaaaacg caaagtaatc caacaaaaga aacaagaagc
     6061 acaatggatt aaggcatttg aagagggcaa ttgttcactg gaagaactca agcaacaact
     6121 gcaagctctt caaaagccga tgtctacttc ttcgtttctt tcactccaac agtgtgaagc
     6181 aaaagtgaaa gaatggcttc aactaaaaga cctctctagt cgacaattat tatgtgacta
     6241 tgtttgtcgg attgaggtaa caaaggataa aacagtttgt gggatttact tccgacaact
     6301 gacacaaaat attttagaaa ggaacgaatg atctatggaa atgatacaaa ccaaacgagt
     6361 cgcattgtat attcgcgtga gtacacaaga acaagcttta gaaggacata gtattcaagc
     6421 acaagaacaa aatggacgac agtttgccga acggatgggc tatgaagtgg tagaagttta
     6481 tgccgatgaa ggaatcagtg ggaaatccac aaaaaatcgg ttcgcctatc aacgaatgat
     6541 gaacgatgct cggaaccatc gttttgatct actagtgatt tggaaattga cccgtctcgg
     6601 acgcaatatg ttagatgtct tacaaacagt cgaggaatta ttgcgatatg acgtaggatt
     6661 acattcgatc tcggaacaat ttgacgtgac cacttcgaca ggtaaattga tgttacaact
     6721 ccttgggagt ttcggtgaat ttgaacgcaa ccaaattgcg gagaatgttc aaatgtcaat
     6781 gcaaagctta gtgaaagata aaaagcgtta tgcaggaggc cgacgtttag gctatgtcag
     6841 tggtattgac acgaatggtc aaaaacaatt ggtcattgaa ccagaagagg cgaaaattgt
     6901 tcgtctgatc tatgcaaaat atttaggcgg agcagggtat cgagcgattg ccaatttttt
     6961 gaatcgtcaa ggatatcaaa cagtcaaaaa gaatgctttt tctaccacgg cagtcaaaga
     7021 tatcttacgc aataaaattt atgcagggta tttagaatat gcgcgctaca tcaattggga
     7081 aaccaaacga cggaagggga agaatccgag accgatttta gtgaaaggaa cccatgaacc
     7141 gattatttcg gaagaagatt atcaaaaagt acaagaacgc cttgctttag aaggaaaaca
     7201 acccaaatgg aaccatacag gtgaaaatgt attgacaggc ttgttacgtt gtccagaatg
     7261 tggcgcaccg atggcagcga gtaatgtcac aaatacccta aaggatggca caaagaaacg
     7321 gattcgatat tattcttgtt cagtctttcg gaataaagga gcaagtgtgt gtcatgccaa
     7381 ttcgattcgt gccgaacaag cggaaacatt tgtcgccgac cgattaaagg agatcgttca
     7441 gctaccagaa gttcttcccc gattggtggc agcgttaaat gaagaaatag tgcgacaaag
     7501 ccaaccattg gaacaagaac tcgtggtgtt actggaacga aaggaagaat tgaagactaa
     7561 aattgaaaaa tgggaagctg ctttggaaga tagtcctgaa ttgtttccga tgttaaaaga
     7621 ccgcttagac gaattgactg aaaaacgacg acagttgcac attcgagaaa atgaaatctt
     7681 agggatcttt caacaaggag agccgattca agtcaaagat gtccaacgga tattgactaa
     7741 tttggatcgt tttcttgccc agagtgagaa gaagcaaatc aaagccttgt atcgtacctt
     7801 tatagagaaa ataacctttg atcctaacca taaggagatg ttggaaatta cgatgaaatt
     7861 cacgcatgcc gtggtacaac aattgaatga gcaatatcaa atagccgtat ccaaaacaaa
     7921 ggatacggct atttttgtgt taaaaacacc ctttacgtta acgatctagt ctaggttata
     7981 tcaaacaacg ttagaggggc gctaacgctt ctctagcgat ttgaaatcgt tagagaagta
     8041 gttagggagg tccttatcat aaatagggat atttattaat gaatatttga aatataattg
     8101 tatataagaa ttaaaaagag tggggataga tatgaaaaag atgggtaagt gtattttatg
     8161 tgaaaaatac actgaactta ctaaagaaca tgtaccacct aaaaaatcag ggaatactgg
     8221 aggtaaaaag accagaactg gtaatttaga tgatttcctt aagagcgatt ttacaaaagg
     8281 tgattttccg aaggggatta aaaggaaacc tcaaggaaat gtttattata
//
LOCUS       DAJSBM010000094         8042 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJSBM010000094 DAJSBM010000000
VERSION     DAJSBM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.097"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(80..928)
                     /locus_tag="ONT67_002534"
     CDS             complement(80..928)
                     /locus_tag="ONT67_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2356245.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1152..3092)
                     /gene="thrS"
                     /locus_tag="ONT67_002535"
     CDS             complement(1152..3092)
                     /gene="thrS"
                     /locus_tag="ONT67_002535"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR2356246.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3429..3608)
                     /locus_tag="ONT67_002536"
     CDS             complement(3429..3608)
                     /locus_tag="ONT67_002536"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCR2356247.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3655..5478)
                     /gene="cadA"
                     /locus_tag="ONT67_002537"
     CDS             complement(3655..5478)
                     /gene="cadA"
                     /locus_tag="ONT67_002537"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2356248.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5756..8035
                     /locus_tag="ONT67_002538"
     CDS             5756..8035
                     /locus_tag="ONT67_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356249.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
BASE COUNT         2358 a         1707 c         1455 g         2522 t
ORIGIN      
        1 aacactttga tggatcattt atttgtggaa aactacgttt tgttattcta aaaaacttat
       61 ataaaaaata gtaaggtttt tatttcgaat ccataaaata ttcttggatt gcttctcgtg
      121 tttttttcac tttttcgcta agcttttcgt ttttagccat atagaaataa aaaatgatat
      181 cgatcgtagc aaattgggcc aaaagtgaat tagttgccgc acttcgattc gtgatttctt
      241 ttggtctaga tgtttgtacg agtacatcgg ctatcttgct aagcgggtta tttccaaatt
      301 gcgtcaacgc aatgatttcg atattcatgc ttttcgccag ctcggctaat gcgactacat
      361 ctgcacttct acctgagttt gaaacaagcc aaagcacttt ctttttctca ttgctgacaa
      421 gttgcgggag caagacgtgg atatcttttt caaaaatgat tggcttaccg acacgagacc
      481 atttttgcag aatatcttct actactaaag aagaggcgcc aaccccatat aaaaaaacag
      541 tctctgccga ttccaaacac tctacgactt tttggatcgt ttcttcttct agaatatcta
      601 ctgtttcttt tatcgctact tgtgcattgt taccaagttt gtttttgata gagtaaaagg
      661 actcatttgc ttctatatca ctaaaacctt catcgattcc agtctgtagt tctgttgaaa
      721 tcgtgatttt gaagtccgtc agactttgaa aaccgatttt tttggaaaaa cgtacaactg
      781 tcggcgcact caacccggaa gcttctgcta attcattagc tgtcattgta gggaccttct
      841 caatattacc gataatgtag ttggccaact ttttttctgc tgaagaataa tcttccatca
      901 tactttcaat acgactaatt acgctcataa gttcctccac ccttttaatg atagtctatg
      961 taagtatacc atatcagacc cttattcacg gtcgtatact ggataaaaaa caaaaaaaca
     1021 atccgcccaa cagaatatct tatcccatca atcccacaga ttctattgag catctgtgtc
     1081 tttgaggaat acctgttatc tattgttcgg attgctttct atctactact tttttacagg
     1141 aaatttacct attagttttc tctgctgtaa tttttgactt ctgcttccat agcatcaata
     1201 aagatattca aatcttctac agctgtttct ttacttccat aacgacgtac gttcactgtt
     1261 gcatcttcca attccttgtc tccaacaact aattggtaag ggatcttttg tgtttgagag
     1321 gcacgtatct tatagcccat tttttcatta cgatcatcaa cttctacacg caaccccttc
     1381 atttgcaaac gttctttgat ttcataagca taatcgccat gtgcatcaac agaaacaggg
     1441 atgattgttg cttgtatagg tgccaaccat gttggaaaag cgcctttgta tacttctgtt
     1501 aaataagcga caaaacgttc cattgtagaa acaattccgc ggtggataac tactggacga
     1561 tgcgtatttt ccccgtcttc tccaacatat gtcaaatcaa agcgctctgg caataagaag
     1621 tctaattgta tagttgataa tgtttcttct gtaccaagag ctgttttgac ttgaacatct
     1681 agttttggtc cgtaaaaggc tgcctcgcct tctgcttcaa agtaatctag ttctaattca
     1741 tccatagcgg ctttcagcat tgcttgggct ttttcccaca ttgcgtcatc atcaaaatat
     1801 ttatctgtat tgtttggatc gcgatagctc aagcggaaac gataatctgt aatattgaag
     1861 tcagcatata ctgccaccat caattcaagc gtacgtttaa attcatcttt gatttgatca
     1921 ggacgaacaa atgtatgacc atcattcaac gtcatttctc gtacacgttg taaacctgac
     1981 aaagcacctg atttttcata gcgatgcatc attcctaatt cagcgatacg aattggtaat
     2041 tcacgataag aatggatcgt gtttttgtag accatcatgt gatgagggca gttcattggg
     2101 cgaagaacaa gcatttcgcc atctcccata tccattggcg ggaacatgtc ttcttgataa
     2161 tgatcccagt gtccagatgt tttgtacaac tcaacatctc ccattattgg tgtatataca
     2221 tgttggtaac ctagactaac ttctttgtct acgatataac gttcgatcgt gcgtcggatt
     2281 gttgcccctt ttggaagcca gaatggcaaa ccagaaccta cttctgggga aaccatgaac
     2341 aagtcaagtt cttttcctag tttacgatgg tctctttctt ttgcttcttc acggagacgg
     2401 atatattctt tcaaagcttt cttgtcaaaa aaggctgtcc cgtatacacg ttgcatcatt
     2461 tgattgtttg agtttccacg ccaataagca cctgccactg ataataattt aaatacttgt
     2521 atacggccag tggatggaac atgtggtcca cgacacaaat cgatgaattc gccttgttga
     2581 taagcagtaa tcacttcttc ttctggcaat tcactaatca gttctacttt gtacggatca
     2641 cttgcaaaaa tttctaatgc ttcctgtttt gaaagaacac ggcgttcgat cggcagattt
     2701 tctttgacga ttttcatcat ttctgcttcg attgccggca aatcttctgc agttacttgg
     2761 ttttgtccat tatctgtatc gtagtaaaac cctgaatcga tagctggtcc aacgccaaag
     2821 tggatatttg gatagaggcg acgcatggct tgggccatca aatgagctgc tgaatgacgg
     2881 actaagccaa gtgcatcttc atggtctggt gtgatgatct caatacttgc atcttcctcg
     2941 attggacgag tcaaatcaac gagttttccg ttcactttac cagctaaagc tttttttgct
     3001 aagctttttg aaatgctttc agcaattgcc aatgttgttg ttccagactc aaattctttg
     3061 actgcaccat ctggaaaagt aatttttatc ataatagttt cctccttgaa atgttttgat
     3121 tgatcagatc atcaagctga aaaacaaaaa aatcctagca tctcttgaaa agagatacta
     3181 ggacgaaaag ttcgtggttc cacctaatgt tcaaacgcaa atgcgttcct caagcgttgt
     3241 aacggtccga ccgcccttgc ttcaacaagg gtttcgaaag tggtcatcct ttggttttct
     3301 cctggaaaag tttcagccta gcttttcctc tcttatcgaa gactctcaaa ttcagttggc
     3361 tttctcatca atcaattatg tgtttattta accactaata aatagaaaaa tcaagtggga
     3421 aactaaactt attttttcca ttccccattt acaccgtatg ttcctttttt catctcattg
     3481 atgatgacat ggatgtgttc ttttggtgcc tgcgtgtttt tatgaacagc ttccgtgata
     3541 tctttcatca tattttctaa ttgttccgga ctgcgacctt ctactaattc gacatgtacg
     3601 aatggcataa tgctctctcc tctttctgat cgttcttttt tcaactcgat tctatcatac
     3661 tatgtttgtc ttgggtactg aatcaggaaa aatggctgga tctttttttg ctctcaaggc
     3721 actcaaaata gagaccgtat ccacgacttc ttgcaaaaca gctccgaata atgcggggat
     3781 tagtcccgta cttgcaatta acatcaaaaa aatacaaata aagatgccaa tcagtacaga
     3841 ctgtttcgct attctcatcg tatctcttga aatcactact gcttttgcta cacgcaagag
     3901 atcatctttc aagatgaccg catctgcact ttcactagca gctgtagaac catgagcccc
     3961 cattgcaata ccgatatctg cagcagcaag tgctggtgcg tcattcacgc cgtctcctac
     4021 catcgcaaca ggtctttgat ctttagggat actttctaaa atggtcagtt tatcttgagg
     4081 caagctacgg gcatgtacag aatctaattg gactttttct gcaatcgttt gagcaatggc
     4141 agatccatcc ccggtgatca tcatcaaatt tttcacatca agttttctta gctccttgat
     4201 tgtctttgct gcttctgggc gaatctgatc cgaaaatagg atcgttcctg caaaagatcg
     4261 atcaattgtt acatagattg ctgtattgga cgtttctatt tcttcagctg aaacaaaaga
     4321 ggcacgtcca atctttactt catgattatt gactactgct tggattcctt gaccggtcac
     4381 ttctttcagt tgactaacag gatacagttg gctgtttttc acgttgttca ctaacgaacg
     4441 agctaagata tggctagatt cctgttctgc actagctgca tacagaagta gctcttcttt
     4501 ttttattcca ttgactggct gaatcgcctc tacttccaat atccctttgg taagcgttcc
     4561 tgttttatca aaagcaatcg tttttgtacg tgcgagcttt tcaatcgttg ttccagtttt
     4621 tacaacgatc ccgtttttac ttgaacggct cattcctgca actaaagcaa tgggcgcagc
     4681 tagaatcagt ggacaaggtg atgccactac cagtacctca gcaaatcgta aagggtcttt
     4741 tgagaaccac caagaaatac caccgatcaa ataagcaatc aacgtgaaag ggactgcata
     4801 acgatcagct aaacggacaa atcgggcagg cttttctttt gattcttcga ctagtttaat
     4861 aatcatttga tactggctgt ctgcggcacg tttttcagcg atcatttgga tcgatccgtc
     4921 tccatttaaa gaccctgaca taatctgatc tccaggtttc ttggtttccg ggcgggactc
     4981 acctgtcaaa gaagattcat cgaccgaaga ttctcctttg gtcactcgac catctgcggg
     5041 tacaagttcg ccaggcttga ctagtaactg gtcatcaata tttatttcat cgattactat
     5101 atcctgcaat tttccttctt tgaatcgatg ggcaattctt ggggtgttat ctaacaaaga
     5161 ctgcagttct ttacccgctc gtttattcgc gtaatcctct agactatctc cgcccgtaag
     5221 catgatcaat atcatcaaac tagcccagta ttctttgacg gctaatgttg caaggattgc
     5281 ggtaatcgct aatacatcta cgccatatct tcctgaacgt aacgtcttga tcatttccaa
     5341 aaacatcaag aaggcgagaa tccctccagt aaatgcgaca atcgcaaatg caagggccgg
     5401 ctggtgaaaa acaaattcac ttaataaagc tatcccacca ataagcaaag taagtaaaaa
     5461 ttttttgaaa tgtgtcattg ttttttctcc tcttttatgt gacttagaga ggttagtaat
     5521 aaatttccag ctgcctcttt catcttaagc atagtccttg actagcactt tttaaaacaa
     5581 taggccttaa ttgaaaacaa ctatcactat cagcgtttcc aaaattcatc agttctacaa
     5641 acaaaaatat tcagcaagga ttactttttc gattcatcag ttaaaaagta ccttttttcg
     5701 aaaaactatg ctatactttt aattcgttat ttacggcgga aggagcaaac atgcgatgaa
     5761 tgagaaacaa ctagaacaaa cgcacgtgga tgaaacgatc gaactaatcc agatcgaaca
     5821 aaatatgctg gatacaaaac aacaagaatt gactcaggaa atgaatcatt cgacaaaaga
     5881 cacagctaat cacaagattc gagctggttc caatgaagct ttttatgaat ctgctgttga
     5941 atatcgccaa catgagcagg aattgctttt gaaataccat actcttgaag cacagcaaaa
     6001 acgcctgcaa acgttgtctg tcatgaaaga tagtccttat tttgcccgta tcgactttaa
     6061 agaagaccgt gaaacagaaa cattatatct aggtatcgct tctttaagag atctggcaga
     6121 agaaacaatc gttattgact ggcgcgcacc aattgccaat ctttattatg aaggtgagat
     6181 tggtcccgca ttttatgaaa ctgaaacaga tagaatagat gtcgatctgc agttgaaacg
     6241 acaatttaaa atccaagaag gacgtatttt atctatggta gatacgtcgg aagtaatcaa
     6301 tgatgacttt ttgctagaga tattggatga atcatcaagc aaccatatga aaaacatcgt
     6361 ctctacgata caaaaagcac aaaatgcaat cattcgggac acaacaagta aagtaatgct
     6421 gattgaagga atcgccggaa gtggaaagac atccgctttg cttcaacgta ttgcttttct
     6481 gttgtatcat aatcgcaagt ggctcgaggc tgaaaacgtt cttctatttt cacccaacca
     6541 tttgttttct gattacatct cgactgtatt gccttcatta ggtgaaagcg gcgtgcctac
     6601 acagacattc aaaaattatt tggtgcagct gctgccagag ttttctttac tagatgaaca
     6661 gcaacaagaa agtggttttt tgctagggga aaaagatccg atacaagtga tgaaaagcgg
     6721 attgacctta gtcgatcaga ttgatcgcta tatccaaggc atcacaaact acggtccctt
     6781 gttccgtgat atgaaaataa atggccgtac gatcctatca aaggaatcga ttcgccaatg
     6841 gtataaagaa acaaatgaac aattaccttt acatcataga ctttctctat tacaaacgaa
     6901 gctcctgaaa aaattaggcg gtctgcaaaa agacgaaaca cgtcagcaat gggtcaaaga
     6961 tctggcggaa gaacagctcc aagaactata cgcaacagat ccaaatcttg aatatacaga
     7021 acaaaaggag cgttcattac gtaaaaaact ggccaaccaa atcgtacgta aaagattccg
     7081 tcgaatccat cgagggatca tgaattatca atttgtcaat ataacgaaac aatatctcca
     7141 ctttctacaa actgtgccac tttctcttct aacacgacat aaaatcagcg agaccaggtg
     7201 gcaggaacat ttgacaactg ttcgtacaca cttgcgtcaa agagaattgc gtcaagagga
     7261 tgctgtacta ttctttttac tgatgcgccg aacatatcca gtgacggtta cacacaaagc
     7321 tcgctatatc tttattgatg aaatgcagga tttcgcacca tcacaagtgg cattgctgca
     7381 agccttgttc ccaaaagcaa atttgacttt ctgtggagat ctaaatcaaa atgtctttgg
     7441 aaatgaaacg atcgttggat cattggaaga ccttttcgtg gaccgtgaag tcactcgttt
     7501 ccaattaacg acaagctatc gctccacaaa agaaatcact gattttgcta atcattttat
     7561 tgtgaaagaa catcaagtag aaacaacagc aagaaaagga gaccttcctt tgctcatcca
     7621 aggaacaaca gatgatatca aattgaattg gttagcagat accattcgaa cgactcaggg
     7681 aaaagcccgg tattggcgta cagcaatcat agctaaaaca caagaagaat gcgaagctct
     7741 ttatgctcaa ctgccagacg agttgaaaca acaagtccag ctgattttaa atgagagtga
     7801 ctttatgaag cgttccatta ttttacttcc agccttttta gcaaaaggtc tggagtttga
     7861 ccgagttttc ttgtgggatg tagatgcaga acatttttct tctcagcaag acaaattgat
     7921 cctttacacc atgtgtacac gtgcgatgca tgaattgacc ctttttacct ccaataatga
     7981 aagtcctttg ttaaacaata tggatatttc atcttatgag aaaaaagtac tgtaagtaaa
     8041 aa
//
LOCUS       DAJSBM010000095         7649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJSBM010000095 DAJSBM010000000
VERSION     DAJSBM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.098"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..179)
                     /locus_tag="ONT67_002539"
     CDS             complement(<1..179)
                     /locus_tag="ONT67_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR2356250.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="ONT67_002540"
     CDS             complement(192..1295)
                     /locus_tag="ONT67_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2356251.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="ONT67_002541"
     CDS             complement(1385..2341)
                     /locus_tag="ONT67_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCR2356252.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="ONT67_002542"
     CDS             2622..4556
                     /locus_tag="ONT67_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR2356253.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="ONT67_002543"
     CDS             5013..5429
                     /locus_tag="ONT67_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCR2356254.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGNYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="ONT67_002544"
     CDS             5443..6009
                     /locus_tag="ONT67_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCR2356255.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="ONT67_002545"
     CDS             6032..7018
                     /locus_tag="ONT67_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCR2356256.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="ONT67_002546"
     CDS             complement(7173..>7649)
                     /gene="scm"
                     /locus_tag="ONT67_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCR2356257.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2193 a         1466 c         1577 g         2413 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggaaattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgct
//
LOCUS       DAJSBM010000096         7630 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJSBM010000096 DAJSBM010000000
VERSION     DAJSBM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.099"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(60..788)
                     /locus_tag="ONT67_002547"
     CDS             complement(60..788)
                     /locus_tag="ONT67_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2356258.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(801..2081)
                     /locus_tag="ONT67_002548"
     CDS             complement(801..2081)
                     /locus_tag="ONT67_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2356259.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2222..2824)
                     /locus_tag="ONT67_002549"
     CDS             complement(2222..2824)
                     /locus_tag="ONT67_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCR2356260.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3069..3275
                     /locus_tag="ONT67_002550"
     CDS             3069..3275
                     /locus_tag="ONT67_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356261.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3587..4306
                     /locus_tag="ONT67_002551"
     CDS             3587..4306
                     /locus_tag="ONT67_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCR2356262.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4387..5070)
                     /locus_tag="ONT67_002552"
     CDS             complement(4387..5070)
                     /locus_tag="ONT67_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCR2356263.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5288..7474
                     /locus_tag="ONT67_002553"
     CDS             5288..7474
                     /locus_tag="ONT67_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2356264.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2733 a         1284 c         1438 g         2175 t
ORIGIN      
        1 aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt
       61 tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac
      121 gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat
      181 ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat
      241 tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa
      301 aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag
      361 atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg
      421 acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg
      481 ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa
      541 ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt
      601 tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt
      661 tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta
      721 tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg
      781 ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg
      841 ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata
      901 aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac
      961 tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc
     1021 ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca
     1081 ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg
     1141 attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt
     1201 aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc
     1261 ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat
     1321 ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca
     1381 tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc
     1441 atcctgactt gttgctctaa taactgatca gctaatttct tattagagat tttccaataa
     1501 taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct
     1561 aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat
     1621 aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct
     1681 aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga
     1741 aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgtcg
     1801 atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc
     1861 aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt
     1921 agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga
     1981 atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt
     2041 gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga
     2101 tttttaccaa attatagcac aattagcgta ttattttttt aatttttcag ggattttgaa
     2161 ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg
     2221 tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag
     2281 gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag
     2341 ctgagaaaaa taaagtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata
     2401 atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa
     2461 gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt
     2521 gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa
     2581 cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa
     2641 aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta
     2701 gttttccaag attattcaat aaaacttcta aggcaaattt tacttggaca tacaccatat
     2761 cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt
     2821 tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg
     2881 tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt
     2941 tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag
     3001 tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag
     3061 ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg
     3121 atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga
     3181 tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt
     3241 tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata
     3301 aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct
     3361 tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt
     3421 tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac
     3481 ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc
     3541 tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt
     3601 gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca
     3661 agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt
     3721 ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt
     3781 tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga
     3841 agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa
     3901 gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa
     3961 gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa
     4021 aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa
     4081 agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt
     4141 gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca
     4201 agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta
     4261 cactacaaat aacccgaaag caaagggaat ctttacaaat aaataatata aaaactaagc
     4321 aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca
     4381 ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt
     4441 gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg
     4501 atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg
     4561 gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc
     4621 tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc
     4681 aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg
     4741 ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa
     4801 caagcgccag aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag
     4861 aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc
     4921 gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc
     4981 atattggata taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat
     5041 tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg
     5101 tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc
     5161 atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac
     5221 gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat
     5281 caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa
     5341 caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga
     5401 agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga
     5461 aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga
     5521 acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg
     5581 gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt
     5641 agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa
     5701 acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat
     5761 cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat
     5821 acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat
     5881 acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt
     5941 agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa
     6001 atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct
     6061 cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca
     6121 aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga
     6181 ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg
     6241 gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta
     6301 tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa
     6361 aactcttttc ttattgacta attatcgttc tcgggcagct attgtccaag gagcaaatag
     6421 attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga
     6481 agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat
     6541 caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata
     6601 ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt
     6661 tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt
     6721 ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta
     6781 ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca
     6841 atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc
     6901 caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga
     6961 ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa
     7021 ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt
     7081 cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg
     7141 cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag
     7201 taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta
     7261 taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtgac
     7321 gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct
     7381 gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa
     7441 agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg
     7501 tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt
     7561 cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga
     7621 caagaatttt
//
LOCUS       DAJSBM010000097         7597 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJSBM010000097 DAJSBM010000000
VERSION     DAJSBM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.100"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(940..1353)
                     /locus_tag="ONT67_002554"
     CDS             complement(940..1353)
                     /locus_tag="ONT67_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCR2356265.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1809..2294)
                     /locus_tag="ONT67_002555"
     CDS             complement(1809..2294)
                     /locus_tag="ONT67_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356266.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2433..2648)
                     /locus_tag="ONT67_002556"
     CDS             complement(2433..2648)
                     /locus_tag="ONT67_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCR2356267.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2872..3672
                     /locus_tag="ONT67_002557"
     CDS             2872..3672
                     /locus_tag="ONT67_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2356268.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3691..5559
                     /locus_tag="ONT67_002558"
     CDS             3691..5559
                     /locus_tag="ONT67_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR2356269.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5552..6043
                     /locus_tag="ONT67_002559"
     CDS             5552..6043
                     /locus_tag="ONT67_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR2356270.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6031..6585
                     /gene="srlA"
                     /locus_tag="ONT67_002560"
     CDS             6031..6585
                     /gene="srlA"
                     /locus_tag="ONT67_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR2356271.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6603..>7597
                     /locus_tag="ONT67_002561"
     CDS             6603..>7597
                     /locus_tag="ONT67_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR2356272.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2234 t
ORIGIN      
        1 gttactatga ataacataat aagaaagtaa agtggattga tttgagatct ttttatactt
       61 caaaaaagat aacacccaac tttcaaaaat atgttttgga agttgggaca aacgagttaa
      121 tacattttaa tacattattt tttattcaaa aaaaatagca caatggcaaa gatcgtaaga
      181 gagatcataa gccccaatga atgactaaaa agaaaagcag aatcacatgc catatcatcc
      241 catatttctt caagaaaata aataagcggg aaaaagggaa ggaatagtag aacatcgatc
      301 cagcaccatt tttttcttct tttgaaataa cataaaacaa taaacatgaa cgtgcaggga
      361 agtaaaaaca aagctttact cataactaac aaaataccag ctccttttat ttttttctcc
      421 cccacattca aagagtaaca atggtatgta cttaataata gaaaaatgga tcaacttgta
      481 tgaataacag ctgaaaagat ctgcaccaaa taaaaaactt ccagccaaaa ctgactggaa
      541 gtttattttt ttctgaaata ttctaacaca aatatgaaaa agatggccga agctaataag
      601 ccgatttcgt agcaaaacaa tgagatacca gagtcgtaat aataaaatcc tctaatctta
      661 tggataaaat agatcatcgg acaagcagat aagagtaagt atttgttagg ccttttgaat
      721 aagtaaaaga aagcactggt cattatgata aatggtatcc aaaacaaaag tttacttaag
      781 acaatcaaat gatcaactcc catatttttt accaagagag atcataacat ctttatcgta
      841 aaaacaaaat atttttttgc tcattttgaa aatttaaaca tactcgttct tatccaaata
      901 tataaaaatc gcccctcatt catcgagagg cgataagttc taatttaact caattttttt
      961 tgtttctttt ggctgataag ctgttttagg taataccaat ttcagcacac cgtttttaaa
     1021 ggttccagtg attttttctt catcaacatt aggaagatag aatgaacgtt tgtaagagac
     1081 cgaatgtctt tctttccgta catagttgcc atcttcttta tcttctgtat gggactcatg
     1141 attggcggag atcgtcaaag tattatctga atattcaact gtcgtgtctt ctttgtcaca
     1201 gccaggcata tcagcagtta gtttgtattc tttttcattt tcgactaaat ctacttttgg
     1261 ataacttgaa acacctaaaa aatcattgaa tgctggacta aacacgtcat tgaaatcagg
     1321 gaacatgtcg cgaatgctag gtacatttgc catttttgat catcctttca ataagtattt
     1381 tttatttatc cctatttacg taataactct attttgaaaa aaatgcaagt ataatggtta
     1441 gctttgtgaa tatttcatct aatacgatac aaaggctatc tttctaattt gaaaaaggga
     1501 tcattaaaat aataagaaga aaatatgtca aaaggtaaaa aagtaaattc ttctatccaa
     1561 atatagaaaa attgaatcta gaaaaccgcc cgacaataaa tttcattaac tcagttgacc
     1621 gaaaatgaag tagataccaa aaatttatca agagagcaag tagagaagtg agtatattca
     1681 taagagaaat agctgtaacg aattattagg ttaatgcaaa tgcttattta tactccgtat
     1741 aactttctca gaaaaaatga taccaataat gacttgctag tactgtaaat ccgtataata
     1801 tcatctaatc aaaatggtgt tccatttgca tctgtcatcg taatcgatcc ttcgggtgac
     1861 actcgataaa aaccaacgct tccagatcct ccttgggaac ggatttgttt gctggaagct
     1921 gtgaacaaat aatctcgatc aattttttga taaaagctaa taacaatatc gtcactttgt
     1981 aattcggaac ggtgggcaat aagaaataga agtgctttgt cagagttttc taatggaaga
     2041 gaaggtacag tgctttcttg catttcagat gttgaagaaa tgctaaacga ctgaatactt
     2101 gattggctat gttgcgaatc tacattacca gtagaagtat tagtaatgga agtagtagaa
     2161 gtacttttag ttgaaccaac ctcagttgtt gaaatggctg ctgaagattc tttaacagaa
     2221 ttctttgttt tatatccaca ggcagaaaaa agtattacag ttcccaacaa gcctgcgtaa
     2281 atccctttat gcatgatagc tcctcctaaa atagattatc tttattcagt atatcactta
     2341 aacaaagaaa agcagatggt ttaaaatgaa aaaaccttcc caataaaggg aaggttcatg
     2401 aagataataa cttttttaat aattatagct ttctaatatc tttcatcaac ttttctagta
     2461 atcattggga aaaatttcaa gcagtagatc gcacaaatac ctacgacgat ataaatgatc
     2521 ttcgcaaaaa tagttgttga accaccagca atcatagcta ctaaatcaag ttcgaacaag
     2581 cctaccaaca gccagttcaa ccctcccaca atcaataaag ttaatgcaat agcatctaaa
     2641 gttttcatta gaattctcct ttcttaaatg ataaaacaat tatatatcaa aaagagaaac
     2701 attgcttata taacgctcag gaattttagc aatctatgca aaaagatgaa aatttttttt
     2761 gctcttccat tagtacacat tttttagctg aagctgtaaa tttcatgtct taattttttt
     2821 tagtgagctt ttatactgga tttgtaagaa aaaagaagga gaggatcaca tatgtcggat
     2881 tggttggata ttgcaggaaa agtagtaatt gtaactggag gttcctcagg aattggaaga
     2941 agtatcgtcg aaaatttgtt gaagcagaat gcacaagtag cgaattttga tgtaactgaa
     3001 tgccgtatac agcatgaaaa cctactatct ttaaaagtcg atgtgtcttc taaaacggac
     3061 ataaaagaag ggatatataa agtaatgaaa cattttaaga cgatcgatgg tctagtcaat
     3121 aatgcaggaa taaatattcc ttctcttcta atagatagaa atcatccaaa aagcaagtat
     3181 gaattatcag aacaagtttt tgataagatg atcgctgtca atcaaaaaag tgtatatttg
     3241 atgagccaag cagttggaag aatcttggtg caaaagggat caggagtgat cgtgaatctt
     3301 tcttctgaat caggattgga aggatcagaa ggacagagct gttatgctgc tacaaaagca
     3361 gcgatgaaca gttttactcg ttcttgggca aaagagttag gaaagaggaa tgttcgtgtg
     3421 gtaggtgttg caccagggat actggaagaa accggtttga gaacacaaga atatgaagaa
     3481 gctctttctt atacgagagg tataagcgtc gaacagctga gaaacggtta cagtcgaaca
     3541 agtacgatcc ctctaggaag gagtggtaag ctgcaagaag tggcggatct agtctgctat
     3601 tatctttcag aaagatcaag ttatatcact ggagtcacga ctaatatttc aggaggtaaa
     3661 acaagaggat aaaggatagg ggggacgcac gtgacgctag taaacagatg gtaccaatta
     3721 ttgaaattac tagtcactca caagaaagtt tcaatccagg aagtgcaaaa agaaatcaaa
     3781 accagtaatc aaaccataaa aaaagatatt ggggagttga atgaagaaat tgccggtatt
     3841 gcaaaaatca tccaaaaaaa caaacatttt gaacttgaaa tttatgattt tgattttttt
     3901 gatgagatca tgcaaggaaa attgaaaaca gccagtgatt tcaattcggc atccaaaaga
     3961 atcgcttatt tactaaatag gctgatcgaa acagaggatt tcttacgtat ggatgatttg
     4021 tctgaagaag tcggtgtcag cagaggaaca gtggtcaagg atctaaagac attaaaagag
     4081 atgatcaatg attttgatgt aaagattaca ggcacaccaa ataaaggact aagaataata
     4141 ggagatgagc tagaattacg actactttta ttttattttg tttctcccta cttttcagag
     4201 acgaaaacag aacagtttct agaagaaaaa caagcaatca aacattttca gaagaagacg
     4261 aatgctagta aacaagaaat caccctttta acgaaagtta tcggtatttc tttgaataga
     4321 atctctagta actatctgtt gaagaagccc atcaatcgtt acagtgggtg ttttgaatat
     4381 atccaagaat tcaatgaact gatcaatcag ttggaagaaa tctatgaact aactttgagc
     4441 caatatgaaa aagattttat cagttttcct ttgaacatca tcaacacaag tataacgatg
     4501 ataaggaaag ctaactctga tttgcgctta tatttcgacc ccatgatgat gcgtattcga
     4561 cgattagatc tcattgatct ggatgagtcc ttcttatatc aagagatgaa acagcacctc
     4621 tctttattga taaatcgggt aatttttcgt tatcgattga ctgatttatt ttatggtgag
     4681 atagaaaaaa agtatccttt ttcttaccaa atcgctagcg aatgcataca agcattagaa
     4741 gctatcttgc aacataagat ctctaaagta gagatcagct acctaacgtt gtatttggag
     4801 ttagcatttc gatatcaaaa agagcatttt tcctctaaaa aagtagccat tgttcatacg
     4861 acaggaaaag gaacagcatt aatgatccaa agacaaatca aaaggatatt ggggagggat
     4921 atacaaataa cacatgtttc agaagaacaa tatgaaaaag tagatctaaa caaatatttc
     4981 gctgttttta caacgattcc gttaaaaaat gtaaatgaag agacaccggt gatccatttg
     5041 actaatcttt ttgatgactc ttggttaagg gcacaatggc aaaaagcaaa aaaaaagcga
     5101 ttagccgggt ttcattctac tgtttttttc tatcaaatat ggaaagaaaa aaagacgtat
     5161 caagaatatt taaacaaatt agtcgctcct ttgatcagag gctattatgt ggatgaagaa
     5221 tttgaaaaaa ggatcttgaa aagagaatat cagcaaagca catttgttaa caaagaattt
     5281 gcttttcctc acacaaccaa ccagctatct caacacattg ttttatcagt agggctaata
     5341 actgatcaag gtttagcaga aacaggagga aatggagtta tttttttatt tgccatacct
     5401 gaagaaatga cggctgaagt tgagaatgaa ttatttgagc tatacgatat cctatttgaa
     5461 ctcataaaaa tgggaataga tgagtttatt gaattagcac cgacacaaga atcattttta
     5521 aaactaatca aaagtaaagg agagagactt catgaatgat atgtatctat tcatagtttt
     5581 cgcagcttca gcttatgtgc tgcaagcagt tatcagttgg aagcaactaa agcatttcaa
     5641 tcaaacatat agcgaactgc gaagaaaagg aaaagtagcg attggtaaac gacctgggaa
     5701 aatccgttca ggcacgattg ttatgctagc tatagacgat catgatcaag ttttagaagt
     5761 cagaaaaatg caaggggtta catctttggc aaaatttcat tcattacctg tctatgcggg
     5821 tgaagatatc cattacatcg atcgttacca tccctcagtt cgagcggaag atcgtttgac
     5881 acaagcagct ttagaaaatg ccagagaagt atacttacga atagaggcag gaaatgaaaa
     5941 aagtgaacaa ccttcattgc cttttattgc tatgctcaat aattttcgga tatggaaaag
     6001 tacgatcaaa gaaaaaagga gtgacaaacg atggatatca taacaaaatt tgctgaggga
     6061 tttatgaggc tgttccaaac aggtgcagaa acattcataa gctggatgac aggaatcgta
     6121 ccagttgtct tgatgctgat ggttgcaatg aatacgctga ttgctttttt aggtgaagac
     6181 cgagtaacga aagtagccaa attatcagcg aaaaatccgc ttactcgtta ccttgtgtta
     6241 ccgttcttat ctgcttttat gttgggaaat cctatgtcat ttacaatggc tcgtttcctt
     6301 ccagaatact acaaaccaag ctattatgct gctcaagctc aattttgtca tacgagcaat
     6361 ggtgtattcc cccatattaa tcctggagaa ttgtttgtat ggatggggat tgctcaaggg
     6421 atcgaaacct tagggctaaa ttcgatggag ctggctatcc gatacttgct tgtgggactg
     6481 ctgatgaact ttattggtgg ttggataact gattttacta ctggatttgt atgcagacag
     6541 caaggaatcg ttcttagcaa aaaagtagag ctttcagttg attagagaaa ggagagtttt
     6601 aaatgtatgg tgatattaag atcgtaagag gaaacggcgg gtatggaggt cctttgatta
     6661 ttaggccgac aaaagataaa cacaagttca tctatgtcac aggtggaggt gagaggccaa
     6721 tgatcgtaga taaaatagaa gaactgacag gtatggagtc tatcaatggg ttcaaaacat
     6781 caatacctga tagtgaaatc gcgcttgcaa tcgttgactg tggagggact cttagatgtg
     6841 gaatttatcc taaaaaaggg ataccaacca tcaatatcgt tcccacagga aaaagtggcc
     6901 cattagcaca atatatcact gaagatattt atgtttcagc agtagggtta gaacaaatac
     6961 agccactaat ggaaactgat gataacaaag aaacagtaaa tactgcatct ccagataaaa
     7021 agtacaaata tgatacttct aaaaagataa ccgagcaaaa agcagagcag caaaactgga
     7081 tagcaaaaat tggaatgggc gcgggaaaag tagttgctac attcaatcaa tcagcaagag
     7141 aagcagtaga tacgatgatc cggacagtga tcccttttat ggctttcgtt tcccttttga
     7201 ttggtgtgat acaaggttct gggatcggta atgtatttgc taacattatg gcacctttag
     7261 caggaaatgc agttggatta acaatcattg gctttatttg ttcactgcca tttttatctc
     7321 ctttgcttgg tccaggggca gttattagcc aagtgattgg aacattgatc ggtgtagaga
     7381 ttgggaaagg aaatattcct ccccaattag cattaccggc actatttgct atcaatactc
     7441 aaaatgcatg tgattttatt ccagtcggtc taggtttgga agaagcaaaa gctgagacag
     7501 tagaagtagg tgtaccatca gttctttatt caagatttct taacggcatc ccacgagtta
     7561 tcattgcttg ggtagcaagt tttggattat atagctg
//
LOCUS       DAJSBM010000098         7377 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJSBM010000098 DAJSBM010000000
VERSION     DAJSBM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.101"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            123..515
                     /locus_tag="ONT67_002562"
     CDS             123..515
                     /locus_tag="ONT67_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356273.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="ONT67_002563"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="ONT67_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            887..1054
                     /locus_tag="ONT67_002564"
     CDS             887..1054
                     /locus_tag="ONT67_002564"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356274.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="ONT67_002565"
     CDS             complement(1097..1672)
                     /locus_tag="ONT67_002565"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR2356275.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="ONT67_002566"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="ONT67_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2810..4321
                     /gene="gadC"
                     /locus_tag="ONT67_002567"
     CDS             2810..4321
                     /gene="gadC"
                     /locus_tag="ONT67_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HCR2356276.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4334..5734
                     /locus_tag="ONT67_002568"
     CDS             4334..5734
                     /locus_tag="ONT67_002568"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR2356277.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5899..7339)
                     /locus_tag="ONT67_002569"
                     /pseudo
     CDS             complement(5899..7339)
                     /locus_tag="ONT67_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2328 a         1224 c         1312 g         2513 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tccttttttc tatttacaca aaatatttta cactctcaac aaaagtatct
     2461 tcgattaata cttgctcaag ttatgtgcag ctacgaaaaa gtaggtaccg aggtactttc
     2521 tttttcgcag ctttttttgg acttatgaga agtacattcg ctggaagaat acgatataaa
     2581 acgagctttt tcactaaaga tattaataga gtgaaagaaa caagcttttg ttgtcagttt
     2641 tttcactttt ctaaaaatga aaataaaaaa ttctggaagt ggagaagaat tttgcttgct
     2701 atactttgtc ttgtagaaaa gaggtggaca aaataatgca agctcaaata agcgattttc
     2761 cataaccttg gtaggaatga caaattaaat aggaaaggga agtaataata tgaataaaaa
     2821 aaagttatct ctattcggtt tttttgccct gacagcttcc atggttttga ctgtttatga
     2881 gtatccgacc tttgcaacat cacaattaca tttggttttc tttcttcttt tgggaggtat
     2941 tttatggttt ctaccagttg ctttatgtgc tgcagaaatg gcaacagttg aaggatggaa
     3001 agatggtggg attttcagtt gggttagtca aactttagga gaacgttttg gttttgcagc
     3061 tatttttttc caatggtttc aaattacagt aggttttatt acaatgatct acttcatact
     3121 aggagcgctg tcgtatgtaa ttaattttcc agagcttaat acagatccat ttatcaagtt
     3181 tattggtgta ttagtcattt tctgggtact gactttttca cagttgggcg ggaccaatcg
     3241 taccgctaaa attgctaaag caggatttgt cattggaatt gttattcctt cgatcattct
     3301 ctttggttta gcagcagcct actttatcgg cggtaatccg attcaaatgg ctattaatgc
     3361 gaaagctttt attcctgact tttctcagct ttcaacttta gtagtatttg tatcttttat
     3421 attggcttat atgggcgtag aagcttcagc atcacatatt aatgagctgg aaaatcccca
     3481 aagaaactac ccattggcaa tgtttttact agtgattttg gccatcgcct tagatgcaat
     3541 cggcggtttc tcagtagcag cggttattcc acaacatgac ttgtctctca gtgctggtgt
     3601 aatccaaaca tttgaaacgc ttattttaca ttttaacagt catttacatt ggttggtaaa
     3661 agtgattgct ctcatgattg cctgtggcgt tatgggtgaa gttagttctt gggtagttgg
     3721 tccatcacga gggatgtttg cagctgctca gcgaggtctg ttaccagttt tcttccggaa
     3781 aactaataaa caagacgttc cagttccgtt gattatgata caaggaatta tcgttactat
     3841 ttgggcagca gtcttaactt ttggtggagg aggcaacaat ttatcattct tagtagcaat
     3901 ctccttaact gttgtcattt atctcgtagg atatttacta tttttcctag gttactttgt
     3961 attagttttt aagaaaaaag acttaaaacg aacttacaat gtaccaggaa aaactgttgg
     4021 caaaacgatt atcggtggaa ttggttttat tttatcagtg ttcgcattgc ttatctcttt
     4081 tgtaccacca gcttcaattg cagctagtga gcaccgaaca taccaaatca tactacttat
     4141 tagctttgta gtgactgttc ttttaccttt tatcatttat gaagtacatg ataaaagtgc
     4201 tcacacaact attgaggaac ctactcacgt gaaagctgaa gacgttaatc ccgctgttta
     4261 tccagctgct agaggagagc accacgttac aaaaaatgat gaacatattt taaaacatta
     4321 ggaggaattc attatgttat acggaaaaga taatcaagaa gaaaaaaact atttggaacc
     4381 aatttttggc tctgcaagtg aggatgttga cttgcctaaa tataagttaa acaaagaatc
     4441 cattgaacca cgaattgctt atcaattagt acaagacgag atgttggatg aaggaaatgc
     4501 gcgattaaac ttagctactt tttgtcaaac gtatatggaa cctgaagcag tgaaattgat
     4561 gacccaaacg ttagaaaaaa atgcaattga taaatcagaa tacccacgaa caacggaaat
     4621 tgaaaaccgc tgtgtaaata tgattgctga tttatggcat gctccaaata atgaaaaatt
     4681 catgggaact tcaacgatcg gctcttcaga agcctgcatg ctgggtggta tggccatgaa
     4741 atttgcttgg cgtaaacgtg ctgaaaaatt aggtcttgat attcaagcaa aaaaacctaa
     4801 cctggtgatc tcttctggtt accaagtttg ttgggaaaaa ttctgtgtat attgggatgt
     4861 ggaactgaga gaagtcccaa tggatgaaaa acatatgtca attaatctag atactgtcat
     4921 ggattatgtg gatgagtaca caattggtat tgtaggtatt atgggtatta cttacactgg
     4981 tcgttatgat gatatcaagg gtctgaatga tttagttgaa gctcacaata aacaaactga
     5041 ctataaagta tacattcatg ttgacgctgc atcgggtggc ttttatgcac catttactga
     5101 acctgatcta gtttgggatt ttcaattgaa aaatgttatc tcaattaatt cttcaggtca
     5161 caaatatggt ttggtatatc caggtgtggg ttgggtctta tggcgtgacc aacaatactt
     5221 accagaagaa ttagtattta aagttagtta cttaggtgga gaaatgccaa ctatggctat
     5281 caacttctct catagtgcag cacaactaat tgggcaatac tacaactttg tacgctatgg
     5341 ctttgatggt tatcgtgata ttcaccaaag aactcatgat gttgctgttt atttagccaa
     5401 agagatcgaa aaaactggta tttttgaaat cattaatgat ggatcagaat tgccagttgt
     5461 gtgctataag ctgaaagaag atcccaatcg cgaatggaca ctatatgatt tatctgatcg
     5521 tctgttaatg aagggatggc aagtcccagc ctacccatta cctaaagact tggatcaatt
     5581 aattattcaa cgcttagttg ttcgagcaga ctttggaatg aacatggctg gtgattatgt
     5641 acaagatatg aaccaagcaa ttgaagagtt gaataaagct catattgttt atcataaaaa
     5701 acaggatgtg aaaacgtacg gctttactca ctaaaattct tcagagaaat ataaaagaag
     5761 ttaattaact aataaaagga gttgtgatta ccacacagct ccttaaatct atttgaataa
     5821 atatatgata ttcatttgtt acactctcaa tcttagctgt tgttctattc atcattactc
     5881 ttattgtaag tagttcagtc aaatttgctt gttcaattga tcgataacag tatgaattgt
     5941 ttgaaaagta atcggatata taattgtttt ctcactacct aagccttctt ggtataaaga
     6001 actatcgcaa agtataatcc gctgagtaca aaagaaagtg tcgtctacta tttctctaag
     6061 ttgtttacaa atgatattac taatagaaaa gtgttctccc aaaatgtctt ctaactgttg
     6121 tcgatagtat aaatacctaa agtctgacgg agctactatg aacacttcaa ttggagattt
     6181 ccgtttatgc tcatttaaaa gacttaaact aatggttagt ttacgaatag aattctcatt
     6241 ccaacgcaga aataaattgt tttgttttcg ccattgttca agaatttcat gaacttctaa
     6301 gtacaaagcg cgttgctctt catttaacaa gtaagtctta tcaggttgaa atatctgaat
     6361 atcagcccaa agtgagcgga taaacgggag aagtgcttta tcaaaaagta tctgattttc
     6421 ttcagatata ttcagagata agataagttt ttgactgagc tcaatcacca aagggttatc
     6481 tgctacatgg ttttgataaa caacgttcaa gtctttttgt aacaattcaa agttatcgca
     6541 tagataattt ctagagttga atagaaagta gatataaagg cattcatctt cgtcatgaca
     6601 taaagagaaa gaaagttgct ctaatccttt ctgtattagc tgaaacaagg gagtatgtcg
     6661 ggcaactctt ttttcttcct gactaaaata tattgggtta gtcttagatc tctgcaaacc
     6721 gatagcgata cctaataaaa taaattgttt ttcaactgta gggtaacttc ttgaaaaaaa
     6781 atgtttttct acataatcaa tcaattgttg accgctttga ataattgatt tatttagctg
     6841 gatcaactct gtccgatttg tctcatagat tatcgttagt aacaaacttc gataatcctt
     6901 ttcaggaatt tcaaaatggc catcagcatt taagtactgc atttgtttaa aaaaattaat
     6961 caactcattt cgtaagttgt aaactttgct taacgaaatg aaggtagtat cagctatatt
     7021 actccaattg tgttcccctg ataaataatg tcctagtaca ttgaaaaagt cagagcgctg
     7081 atagatgagt tgagtgagtg ccaacctcga aaacgaagag tcgaatgcga ctgtaaagct
     7141 acttctagat ttctccactg aaataatttg ctgatggagt atgtcggtaa tgcattccaa
     7201 gtcgttagtg acagacagtg agtgggcaaa cgttcaattg atctgctatt tcgcgaatct
     7261 gaatggtccc tttttcgtgt aattgctcac aaatgtaaac ttgacgaaaa atacttttct
     7321 ccagataggt ttccaacata gtactcctcc tagtcatttt atacataact attataa
//
LOCUS       DAJSBM010000099         7335 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJSBM010000099 DAJSBM010000000
VERSION     DAJSBM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.102"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..622)
                     /locus_tag="ONT67_002570"
     CDS             complement(<1..622)
                     /locus_tag="ONT67_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2356278.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNH"
     gene            complement(622..1758)
                     /locus_tag="ONT67_002571"
     CDS             complement(622..1758)
                     /locus_tag="ONT67_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR2356279.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(1790..3241)
                     /locus_tag="ONT67_002572"
     CDS             complement(1790..3241)
                     /locus_tag="ONT67_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCR2356280.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(3285..4049)
                     /locus_tag="ONT67_002573"
     CDS             complement(3285..4049)
                     /locus_tag="ONT67_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCR2356281.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4077..4775)
                     /locus_tag="ONT67_002574"
     CDS             complement(4077..4775)
                     /locus_tag="ONT67_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCR2356282.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(4787..5566)
                     /locus_tag="ONT67_002575"
     CDS             complement(4787..5566)
                     /locus_tag="ONT67_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCR2356283.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(5582..6526)
                     /locus_tag="ONT67_002576"
     CDS             complement(5582..6526)
                     /locus_tag="ONT67_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCR2356284.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(6572..7327)
                     /locus_tag="ONT67_002577"
     CDS             complement(6572..7327)
                     /locus_tag="ONT67_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356285.1"
                     /translation="MTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESAITNQKDTL
                     TSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKESELPKEAVA
                     IQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVTSADVARVR
                     ENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATATYDQYLTVL
                     SQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2018 a         1575 c         1144 g         2598 t
ORIGIN      
        1 gatgattttt agaatatgaa attcttccaa tatgtcccat tacaaacgtc tgacttgaaa
       61 ttcctaattt cattcgtaca tcatttctaa tactttcatc ataataaaat tttttagcat
      121 ccactgcatt tggaatcaaa gtgaatttat catttcgaaa caaaaaatca cctgctactt
      181 ttccacatga aaattcatgt gtcaaaacag acgttaactt acatcgataa tattgcttta
      241 aaagatattt aaaatctcta tctatcgatg tattatggct atgtgctatc cgtatcggaa
      301 tcccagcttt ttttgcagct tttaaaggta aataactcat cgcatcaata tgagaatgaa
      361 caatctcata ttctggatga tccttaaaaa attgttccat ccatttaaaa tatttaggat
      421 aattttgtgg ataaagtctc ggtgcataat agacctttcc tccaagactt attatttcct
      481 catcataatc acttttatat ggacgatgtg tcaaaaaatc aaactgaatt ttagttcgat
      541 ctatattcct ataatagttc attagcatcg tctccaaccc tgcacgatgc atatcattca
      601 cacactgtaa tattcgaatc attatatatt tcccctatac aaatttaatg tttcttgtac
      661 gattttatcc caattatatt tactacaaat aaactcagta gcttgattct tcaaaacttt
      721 aaccatttcc gattgattgc atgcctcttc taatcttatc tttaaatcac ttacatccga
      781 tttcttaaaa atcatagcct tgtcttctac tacttccgta cactctgaga tatttgaaac
      841 tatacaacag ttcccataac tcattgcttc taacaaactt aatggcattc cttctaaatc
      901 acttggaagt gtatagatat acgcattact atataattct tctaattcct gtccttgtac
      961 aaatccagta aatataattc gctcatcacc ttttgctaat tcctttagtt catttgcaaa
     1021 ttcatctgta tcagagcttc ctccggctat tataagtttt ttatctgtct gaacatcttt
     1081 aaatgcctca attagatacc gaattccttt ttcgggaacc aatcgcccca aaaacaaaat
     1141 ataagaatct tttgttaatc catacttttc tgtaatcaat cctgctgatt ttatttcaat
     1201 ttttttaacg ccatttggta taaaccttgt ttttcttcca tatatatctt caaaataatt
     1261 ttggacgctt tcactaagta caattatttc atctgcaaat tttacagcac acttttcccc
     1321 taacatgatg tatgtactag caagcttgtt ccatttttct ctttgatgat ctagaccatg
     1381 aattgttgca acacatcttt tcccaaaaag ttttggcaac cacaacatag cacatggtcc
     1441 ttctgcatga aaatgaacaa catcatattt tccgaatgct gctctaattc caccaaatac
     1501 tgaagaactc attgctgcaa ttccttttat atttaatgta aaaatagtct ttaattttac
     1561 acctttatat tctttatttt tatgctggtc aaattccttt ccgctaacat gatgaccact
     1621 tcgattatag caagtcactt catgcccaag tgcaaccatt cgcgttgcca attcttctac
     1681 cactatctca attccacctt ctcttgatgg aattctttta tgacctagca tacagatttt
     1741 taattttttt cttgccatca cgttttactc cttctatagt atctttatct tacatggaac
     1801 cgtttctatg aagcacagtt ttgactgtcc taaacaaaat tctaaaatct aatcccatac
     1861 tccaattcct gatatattct gcatcaagtt ttacaacctc ttcgaagtct gtgatatcac
     1921 ttctaccact cacctgccac attccagtaa tcccaggttt catcgcaatt ctcgcacgat
     1981 gatgaaaatc atacttctca tattctgact tcaagcaagg tctaaacccc accaggctca
     2041 tttttcccat taggacatta taaaattgtg gaaactcatc caatgatgta gatcgaataa
     2101 attgtccaat tcctgtcttc tttgttccat caggaagtat tttattgcca atgattcttg
     2161 ggtcaaaatc catcttaaac atgagattat tactcatctt attttctttc atcaactctg
     2221 ctttgcgttc ttctgcatcc atatacatac tacggaactt atacatctta aatgtcttac
     2281 cattttgacc aatacgttct tgtgcaaaga aaatcggccc tggtgatttg atgtaaataa
     2341 ctggtgctac gaagataaaa ataacacctg ttgcaataca tccaataatt ccagcaagaa
     2401 tgtcaatcgt tctctttata aatgcttgcc tcatcgtcat aacattaata ctagatgtaa
     2461 taacagtata atcaccaact cggccaataa attgttttcc acgtgtacta tcagacactt
     2521 ttgtaagatt catatgtaca gcaagtccca tttccgaaca tctggcgatt aattctttgg
     2581 gatatggaac actttcgtca acattgatga atacttcatc tacccaattc tgtaaaagat
     2641 attccgctgc attatcagca tttgcaacta ctgggacacc tccgatggta gttccataca
     2701 tatctctgtc tactacaatc acaccactaa gtttatacat gccataattc ttctctttaa
     2761 gattacgaac tacatttttt gcaatctttt gagatgttac aataactaac gatacatctt
     2821 caccgttttt catgcggtga tgcaacaacc ctttccaaag tatacgaatt atatatgtta
     2881 ataatccata caccaccccc attataaata aagaaactct ggaataatca tttccatcct
     2941 gcattgtaat aaggtataaa ctgcacacaa gtaacagcat aaaagattgc tttatagaag
     3001 ctacagcctc tttataatat cctcttttta aaacattttt atatgcctca aataaaaata
     3061 taatcgtaag attctcaagt tctataaaaa ttgccatgtg gcgataaata ggaatcacat
     3121 acggattcca gtcaccctgt cgcattacat atgatatata aaaagcaagc tggacacaaa
     3181 ataagtctaa taaaataaaa tcaatatgtt tagaccaacc acttgagttt tttctataca
     3241 tctctcactc tctcctcatt tcctttttat ctacgaataa agaactaaaa taaaccaaac
     3301 ttcttctttt ttacctcttc atagattgga taattgatct catccccgtt gatcagatcc
     3361 tttgcgactt gtttcatctg tactactttt tccttaccaa actccttggc aatctgctca
     3421 tagcactctt ttaagtaaaa ggtgcgctta gagaccccgt gtgcatcaga agcagccatc
     3481 tgcacgagat tatgtttgac cattgttttg gctgttttct ggatatcttt gccaaataca
     3541 ccaacataac ttggcgcagt aagttgggcc aaacatccca tatctaggaa cggaagcaaa
     3601 tgatttgggt cttctcgaaa atgcgcgttg cgttctggat ggacgatcac tggtatcttt
     3661 cctagttcta acaactcaaa aaaaagccgt tctgcatata acggcacttc cagtgtcgga
     3721 aattcgatca gaagatatgt atcatctaaa tctgtaaata aaatttcatc tcggtggatg
     3781 tcttcgatga gcgttccggt aatacgtact tcttgtcctt ccaataacgt caaaggcaac
     3841 tgtcttttct ctaattcggc ttgtaacgaa gcgactagag aaatgacttg tgatttttca
     3901 ttgctgtatt ttccattatt gtggtgtggt gtgcataaaa tatgcgtaat gccttgctga
     3961 atcgcttttt cagccattgc aatacttgct tctaagtttt ctgccccgtc atcgattcct
     4021 ggtaggatat ggcaatgtag atcgatcatt tctcttgtct cctctcttct tattccttaa
     4081 tttccgtagt aataaccagc atctttgctg tgttctgctc cgttgtatac gacacctaag
     4141 actcgtgctt gtaccatatc taacagttct tttgctttag tcaatgattc ttttcgtgac
     4201 gtattttccc gtacgaccaa taaggtgcca tccgtttttg aagacatgat ctgtgcatct
     4261 gttacggcaa cgactggcgg catgtcgaag atcactacat caaatagttg tcttgcttct
     4321 gctaaaatct gatccattct tggcgaactt agtagttctg atggatttgg tggtttaggt
     4381 ccacttggca gaatcgacaa attgtcgact ggtgttcgtt ggatctcatc tgctacactt
     4441 cctgatgaac taagaacagt acttaatccg cttgcgttgt tcaacttgaa cgttttatag
     4501 atgacgggct tacgtaagtc tgcatcgact agcagcaccc gttgaccaga tttggcaaaa
     4561 acaacggcaa tgtttgctgc agtggtcgat ttcccttctc caggtcctga tgaagtcaca
     4621 acaatcgttt tgatttgctg accagctgcg gaggcaaact ggatattcgt ccgaatcgta
     4681 cgatattgtt cagcgattgg tgaagaggga ttggtgagtg tgaccaaact gactgcatga
     4741 gtttgggctg ctttttgttt ctgtgttcgt gccattttct ttcctcctag actcttgagc
     4801 ggcttctgcg tgatggcgct gttttatcgt ttgtagcttt tggttgtgtc tgagctggtc
     4861 cttttttacg gataccagca ttcaattctt tcgctgacat ttctggaact gttcctaaga
     4921 ttgggaatcc taatgtatct gtcacatatt tttcatcttt cactgtccga tcgagtaatt
     4981 ccaataagaa tgccagacct acacctacca tcattcctaa cattaagcca gtcattaaat
     5041 tcaatttatt atttggcgaa acaggtgtcg tattggcaac tgcacctgaa atgatcgaga
     5101 ttttatcaac attcatcaca tcttttgcgt tctcttggaa aacttgtgaa gttgtgttag
     5161 cgatctgttg agcagccact gcattcgtac ttattgcctg aatcgagaac atttgtgaat
     5221 tttctgcctg attaactgag atggcatctt tgatttgttc agcagtcatt ttcacattgt
     5281 cttctgtttc taaacgatct ttgacttcat tgagcactaa atcactgacg atcatatctt
     5341 tatacgtatt gatcatttgc aggttcgtat tgacatcatt gacatttgca gtgtctgatt
     5401 gtggcagtgt cacgatcaat tgtgcttgtg aactgtattt tggtgtcaag atgaaaaatg
     5461 ttacgattcc tgagatggcc aagccagcaa acattgagat gatgattatt accaagtgtt
     5521 tgcgtaatgt ttcaaaaatt tctttcaagc tgattgtctc ttccattttt tctccacttt
     5581 cctatttatt ctctagattc atttgatctt ttaattcttt tttcacacga tcaagttctt
     5641 gatcactgac tttttgatag gagatattat cttgcatgaa gccttctcct tgcagctgat
     5701 cttgcttaat cgtaccaaat gcctcccgat aatctaaagc aatttttttc atttcatcaa
     5761 agttcatgtc tgttttcata ttcgactcta gagcattcaa aacggattga tagttgctga
     5821 caccgtttag actcaatact tttttcgcta cgccttctac gatctttctt tggcgccctt
     5881 gtctaccgta atcaccattt gggtcttcat aacgcatacg agaataagat aatgcctgat
     5941 ccccgttcaa tgtaatttta ccgattggaa agtcatagcc atcttgtgag aaagtgaggt
     6001 cattattgac ttcgatacca ccaactgcat caacgagttc ctttagccct ttcatattga
     6061 tagagacata gtgatcgatt gggatgtcta catatttttg tacggtatcc atcgccatgg
     6121 cagctccgcc gaatgcgtaa gcgtggttga tcttgtcttt tgtgtcacga cctacgatgt
     6181 cgacatacgt atcacgagga atgctgacga tcgttgtctg tttgtcttca ggactgactg
     6241 tggcaaccat catcgtatct gatcttcctt gatcgacccg acctaaatct ccagtatcaa
     6301 tccccatcaa tagaacagag aaagattctt gtttgtccag atcaactggt ttcgttcgtt
     6361 ctccttgggt tcgttccact gattcgtaag ttttctcaat agaatgtgag agatccagat
     6421 ataattttgc tcctactcct acaattgcta atactaatac gataagaact gctaaaataa
     6481 tcagaaatat ttttttccct cttgattttc ttcttcgtct tgccatagtt tcctccgttt
     6541 gctaaccttg cttgaatcaa cgttttaact gtcaatagct tatattgcta tagcccattt
     6601 gctcagcaac agtttgtgct tttgttgcgt atgctgcgct tattttttca ttccggatct
     6661 gctctatttg actgagcacc gtcaaatatt gatcgtaagt agcggtggca gtcacttgac
     6721 catcctttaa catcgtatcg aataactgat ctagttgcgt gacttgcgcg atctgaatat
     6781 cagcgaatcc tagatattgc ataacaaccg ttttccaagg actatcttta aaaaagctca
     6841 tattttcacg tacacgagcg acatctgcac ttgtcacctt ttctttgatg atgacatcgt
     6901 ctacagcttg ctgccagttt gatacatttt tagtaaataa atcgttgaca gctgtctgga
     6961 tcttcagttt tgattctgca ctatctaact gtgtcacgat tgctttcttc tcatcttgga
     7021 ttgcaacagc ttctttaggc aattctgatt ctttgatgtc gtaatcatct gctgaaagtt
     7081 tgacagagga tagcttattt gacaaacttg tgattgtttc ttctgttatt tcagggttta
     7141 gaaaaatttt ttcttttgta ctatacagct tgtctacttc ttttgataaa gaagtcaagg
     7201 tgtctttttg attggtgatg gcagattctg cataattgat ccgttgctga tgttgccgct
     7261 gattattgat atggtatcca aacaaacaga tcgcaatagc acctgcacct accgccacag
     7321 cagtcaatga cttga
//
LOCUS       DAJSBM010000100         6927 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJSBM010000100 DAJSBM010000000
VERSION     DAJSBM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.104"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(45..1511)
                     /locus_tag="ONT67_002578"
     CDS             complement(45..1511)
                     /locus_tag="ONT67_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCR2356286.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="ONT67_002579"
     CDS             1898..2494
                     /locus_tag="ONT67_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2356287.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="ONT67_002580"
     CDS             complement(2552..3034)
                     /locus_tag="ONT67_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356288.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="ONT67_002581"
     CDS             complement(3140..3337)
                     /locus_tag="ONT67_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356289.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="ONT67_002582"
     CDS             3632..4390
                     /locus_tag="ONT67_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCR2356290.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="ONT67_002583"
     CDS             4450..4878
                     /locus_tag="ONT67_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356291.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="ONT67_002584"
     tRNA            4924..4996
                     /locus_tag="ONT67_002584"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="ONT67_002585"
     CDS             complement(5146..5517)
                     /locus_tag="ONT67_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCR2356292.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="ONT67_002586"
     CDS             5732..5917
                     /locus_tag="ONT67_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356293.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="ONT67_002587"
     CDS             complement(6000..6188)
                     /locus_tag="ONT67_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356294.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="ONT67_002588"
     CDS             complement(6212..6475)
                     /locus_tag="ONT67_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356295.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="ONT67_002589"
     CDS             6633..6890
                     /locus_tag="ONT67_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCR2356296.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
BASE COUNT         2294 a         1339 c         1113 g         2181 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatg
//
LOCUS       DAJSBM010000101         6892 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJSBM010000101 DAJSBM010000000
VERSION     DAJSBM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.105"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            568..1479
                     /locus_tag="ONT67_002590"
     CDS             568..1479
                     /locus_tag="ONT67_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HCR2356297.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1966..2232
                     /locus_tag="ONT67_002591"
     CDS             1966..2232
                     /locus_tag="ONT67_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356298.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2304..2645
                     /locus_tag="ONT67_002592"
     CDS             2304..2645
                     /locus_tag="ONT67_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356299.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2700..3119
                     /gene="ssb"
                     /locus_tag="ONT67_002593"
     CDS             2700..3119
                     /gene="ssb"
                     /locus_tag="ONT67_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR2356300.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3196..3492
                     /locus_tag="ONT67_002594"
     CDS             3196..3492
                     /locus_tag="ONT67_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356301.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3507..3938
                     /locus_tag="ONT67_002595"
     CDS             3507..3938
                     /locus_tag="ONT67_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCR2356302.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4120..4425
                     /locus_tag="ONT67_002596"
     CDS             4120..4425
                     /locus_tag="ONT67_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356303.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4829..5317
                     /locus_tag="ONT67_002597"
     CDS             4829..5317
                     /locus_tag="ONT67_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCR2356304.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            5304..6608
                     /locus_tag="ONT67_002598"
                     /pseudo
     CDS             5304..6608
                     /locus_tag="ONT67_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2602 a          944 c         1333 g         2013 t
ORIGIN      
        1 ctcaacgtgg aaaaaccgcc acttatgaga gaaaatggtc aaaaacctaa gcgggaagtc
       61 tccgaataac ataccaagcg ccgacagcca acagataaag aatacactac taaaaatagt
      121 agtagcataa aatttaaaag ggaaaaaatc aacccctacc ccagtcttgg ggtctcaaaa
      181 ctaactcccc cctaaccccc tctttcagcc tcttttgaaa gagggggaga atcaacccca
      241 cggccaacgc cccagtgttc ttggggctgt ccagaacaga aacttgcgag agagtggggg
      301 taaaacaagt gtagaggaaa cttgaagact tgttcgaaca gaaacttgcg agagagccgg
      361 agtaaaacaa gtgtagagga aacttgaaga cttgttcgaa cagaaacttg cgagagagtt
      421 ggagtaaaac aagtgtagag gaaacttgaa gacttgttcg aacacaaact tacaagagag
      481 gggagtaaag cagttgcaga ggaatcagga agatttgttc gaacagaaac ttgcaagaaa
      541 gcattattaa attgaaggtg atgaaatatg aagtatttat tgcatggcta ttttattgat
      601 tattctactc gcaatagtag tactttagcg attctatgtt tgcttcatga attgagagtg
      661 gttactagaa aacagctata caaactattg aattttgaca gatcaattag taaacaagga
      721 gtaacgaatg ctttagtgag attatatgaa aaacagttaa tcgatcgaaa aaaatatgga
      781 acagaaatgt attattattt gacgaaagag ggacatcaaa gcataggtgg gtattatacg
      841 ttgccgaaag ttcctgaata taatttgaat caccatttac aaattaacaa ttatttgatt
      901 aaaggaatgg cattggcaaa agaccaccca catttaaaac ggattatttc agaaagacgt
      961 accgtctatg aaacgaagga tttaaatcct aagacaaaag gaagaaaatt ttttgtgcca
     1021 gattttattt ttcgttttcg agacaaagaa aatcatgaga tcaattggtc ctttgaaatt
     1081 gagctaacgt tgaaaacacg tcgtcgctac cgtgaagcga tttttccgaa atatattcgt
     1141 gagttgcgaa cacgtcctga atcacattta atttatgtga cacctagtgg cgcaattcaa
     1201 gaagaacttc atgcgtttaa gcgtattttt ttcaagcgcg aaactgaaaa agatgcgagt
     1261 caacaagaat tgtttgagcg gttgcatatt ttttcagctt tagagtttga agagggatta
     1321 aaagcattat tagagaatga tccagcagtc aattggcgga cagtagaacg agaaaaaatt
     1381 tatccgaatg gcgctgttca aagtcgtttg aatttatttg aagaatcaac tgaaaatgag
     1441 gaaaaaccaa aagtgaagtt tacgcctgaa gtattgtaaa agagggtgtt cgcttcgctc
     1501 tttttttgtg ccagtttgct ggcaccgttc agacgcaagc gtctgaaccg gctcgttttt
     1561 aggaaggact aacttgtttg atttatccgt tgtcaaagaa gtggacgttc gagagtccct
     1621 aatcgtatag cagtcattcg gcgagtataa cctaaaacat aacctgccaa ttgccaaggg
     1681 taaaatgcac gctgcgcgcc cttggcaatc gtcagaacat gttttcgagc cggttatgcc
     1741 gaacgaccgc tatacgaaaa gggcctctaa acagaagtcc actttgatcc ttgacaactg
     1801 aaacccaaaa tcaaacaagc aggccttcgt ctaaaaacga gccgaatgaa gaccttgggg
     1861 gttgcacccc caaacccccg tgcccccgac acactaccga aaaggtgggg gtgggtggcg
     1921 aaaaaatcaa ataaaacatt ctattcaaga aaggaagaaa caaccatgac aactaaaaac
     1981 gagcaactga aagaacaggc aagaaaagag ttgcaagaaa gaggtttgat tattgagggg
     2041 agctttgaag gagattttga aacctatatt ggttgttatg caagaccgat aaacaaacct
     2101 acagccttag acccaatgaa cgaacaagaa gtaaaagaac aagaaaaaca tgcagtgaat
     2161 ggttttccgc aagatttcac tgaatggtat gagtgggaaa ttaacaacgg gaagcttgaa
     2221 aacttcttat aaaagtcaga tgacgtgcaa agagaatgat tctctttgta cggtaaaaat
     2281 ataaagaaag aaggaaaaag aaaatggcaa aaaacacacc ttatcaagta ggagacattt
     2341 tttatagtag ttggggctac gaacaaacga acgtgacttt ttggcaaatt gtgaagttaa
     2401 cagaaaaaac agcatggttt cagcctattc aaaagaaaac ggtcgagcat tattcgtcca
     2461 tgagtggaaa agttgttcca atgaaagaca agtttataga gtatccttta gcaaaaacta
     2521 aagacagtat tgtaaaaaaa cgaattgatc cagaaaccta tcctgatatg ttatgtggtt
     2581 atcgtagttg ggacacgttt catagatata aaaatcaagc tgtttatgaa agtagttatt
     2641 attaagggga tagtttcccc tttcaaaaat aataaataga agaatggaga gaaaaaatca
     2701 tgatgaattc agtgaattta gtaggacggt taacaaaaga agtggattta aaatacacac
     2761 caaaaggcaa tgcgacaggc acatttattt tagctgttaa tcgaaattat acgaatgcaa
     2821 acggcgagcg tgaagcagat tatatccgct gtgtcatttg gcggaaagct gcggaaactt
     2881 tagcaaaata tactggaaaa ggaagtttga ttggcattaa tggacgtctt caaacgagga
     2941 gctatcagaa tcagcaagga cagaccatgt atgttacgga agttctagtg acagatttct
     3001 atttgttaga gagtaaagaa atcaatgagc aacggtcaaa atcaatggcg aataatcaaa
     3061 ataattcttc aattccagga aatgagattc aaatttcaga tagttatttg cccttttaag
     3121 tgaaaaataa ttttgttcta tacctgattt cttcaggtat agaacaaaaa ataaatagag
     3181 atgaggcgat tttttatgaa aatcattgac agttacgatc aacgaaccat gcaatatttg
     3241 ggctcgtttg attatacaga tgaaaatatt attaattatg ttgcaagttt acctccttat
     3301 caaagcgttc gcttggtaga ttttgcaagt gatgaattgg tattaacaac gatgggcaac
     3361 tttttagatt atgtcggaga caaaaaatgg ttggaaaaaa ttttgtctaa attgattgcg
     3421 aaacaaacag gagaagaacc cataaatgcg attcaattgt tcaatcgata tgaacaagaa
     3481 aacaatgatt aaagaggaga gaaaaaatga aaagattaaa acgaagtatt tttattgtgc
     3541 tagcattgat tgattttgtt gggacaggtt gtgaagcaat tgacgttcaa aaagctccct
     3601 tatcggaaat aaaacaagca ataacaaaac aagatgaaga acaaccgcta aatacacaag
     3661 tagaaatagt gaatcaaaat aaaccgagtt tttctgaaga agatttttcg ttagaaaatg
     3721 gaaccaggca atttttttca gatttggatc gtttaaatcg ggtaggtgta gcaagtgcac
     3781 ttttgcataa aagcaggatg ccaacagaag aacgagaccg attatacatt aagccaactg
     3841 gctggaaaca aaagaaaatg aagaatggcg attatcttta taatcgttgt catttgattg
     3901 gctatcaacg tgcgacaagt ttccccataa gtatgtaata cgaaacggga ggattaatct
     3961 taagattaat cacaactaac ctatttgaca gttggaatga aagctatcat gtctgctgaa
     4021 tcaactggtc tgatactcct acatgcaata aagtctacta ggaagttatt gtatgaagct
     4081 aggtaaaggc ggcagaacaa tttctaagtg tggaatcata tgaagttgga tatgccggga
     4141 cgctataaat tactcagggt gagactgttt ttaattaaac tgacgaactt gcgaatgtac
     4201 gggtctataa ccgacttata ttcgaaagat ataagattgc gaatgcaaag aacattatcg
     4261 aaaagtaaga atgttggtca tgaaattcga gatggaagac tagcggtgta tctgtcaaga
     4321 gttggtaaaa catgtttgat tgattcagat gagagtggga atgcgacaaa acgttttaca
     4381 gaagaaatag agcaactagc agaactgaat atacgatatt tttaaatact ttagcggaga
     4441 ataaaaaaat cctctacaga aaactacgga gggcaacatg tgctattgtt tcaacaatac
     4501 atttttaatt tttaccataa caaaaaagta cttgaaaaaa aacagaaata tgttaaatta
     4561 aaaatgaaaa aatggaagcg gttacttatt cttttgaatc taagataaaa attcaaaaga
     4621 ataacataac ccaaatatga ttatacttga gattctaaat atttatcgaa aaggaatttg
     4681 ttttaaggaa aaatgattct atggacggtt attgatttat atttaccaaa tggctaataa
     4741 aatagtattt cttatattgt atagtttttg tttttatgtg aaaacatgag caatagaatt
     4801 gtactagtag gataaggatg gatggaaaat gaaaaaaact atttttgttt tagcgatttt
     4861 tatatgtttt gggctacttt tggtaggcta cttgttgttt aattctaatg aaatgagaac
     4921 tatttctacg aatgacttgt taaaaaaaag tgaattaaag gataaaaaaa aagaatctct
     4981 tgcctttata atgtttactc aagaagagtg tgagtattgt atagcaataa atccaatcat
     5041 agatagtgta aataaggaaa ctttgaataa gataaagaaa tatgatatat ctgtagattt
     5101 tcaatctaaa caaaagtgta aaattaagtt tgttccgact attgttgcat atagtaagaa
     5161 taaagaagtg gggagaattg acaatgaatt agataatttg tatcgaaaaa agcaagatgg
     5221 ttcaattgta atagacaaga aaaaaacaaa gacattgctt atagattgga tgaaaaaaat
     5281 ggataaacaa ggagaatatt tgtatggaga agattaaaca gacatttccg ttaattaata
     5341 aatattggaa taagaaaaaa atttttatat tttttattac gtttctgctg tcatctttat
     5401 tttctctaat tttcccgtat ctatttagta atgttattga tttatattta gagaatgagt
     5461 tatcacagaa tattgtgcga atagtaacga ttcttatatt tcagttattt ttagtcttaa
     5521 ttaaagaagt gtatggatat ataaaaacta tttatgcatt tgaagttgat ttatcaatga
     5581 aaattgattt tcttaaggtt ttacaaaaaa aaaatctttt tttttcgcaa acgaagcatt
     5641 caggagaaat tcaatttagg atgttttcgg atatttctac tatcgtaaat actatcttta
     5701 ttttttctgt tgaattacct gtgataggcg ttttagttct tgtagcggta tttattataa
     5761 gtaaagtgaa tcttattttt ctgatttttt cactaataat gatgggagct atggtgttac
     5821 agtataattt atttcaatta ccgcttaaga aaacaatcga gaatacaaaa agtgttaatc
     5881 aaagtattac aggagaattg aatgcacatt ttgatcaatt agaaattata agcgcatata
     5941 attttgagaa tatctctatt ttaaaattcg attctagatt taaagaatta tttaatgttt
     6001 tcaaaaaaca aaataaatta ctaacactgg gaagctgttt ggcacaggtt attttccaat
     6061 tatggattgg aggaatattt cttattgatg ttattttatt agctaaagga acaataagta
     6121 ttactaattt tgtaatagta tttatgtacg ctaacatggt ccctacttta tggactactt
     6181 taattagtat tatttggaaa tggcaagact attcagtgtc attaaataga tattacgaat
     6241 attattcaaa taacaacaaa gtggctggca caaaaaaaat aaataaaata aattctatta
     6301 ctggtcaatc attaaatttt tcatataaca aaagaaaggt attaagcaat ataagttttt
     6361 caactgaaat gaataaacta ttcatgatat caggagaaaa tggaacaggg aaaacaacac
     6421 tgatgaaatt atttactaaa cagttgccgg ttagtaataa tcaattattc gtcaacggga
     6481 tagatattaa tatgattgat aacgaatcat taagaaggtt aatttcaatc gcaacacaaa
     6541 taccatatat aattaatagc tccgtgaagg agaatatttt aatggggact aaaaactatg
     6601 ataaaaaaaa attcagtaaa ttaatagaac tattgggaat aagtaaattt atgacgaaat
     6661 taggtattac tttggacgac aatatttatg atgaaaaaat attattatct ggaggagaaa
     6721 aacaaaaaat aagtttagct agggcgctta ttaaatcagc ttcagttatt atattagatg
     6781 aaccatttag aaatttagat aatgaaactc gcgataattt gagggattat atactaaata
     6841 caaaaaaaga agtttgttat ataattataa ctcatgatca caatctagat aa
//
LOCUS       DAJSBM010000102         6643 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJSBM010000102 DAJSBM010000000
VERSION     DAJSBM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.107"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..859)
                     /locus_tag="ONT67_002599"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ONT67_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT67_002600"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ONT67_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR2356305.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ONT67_002601"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ONT67_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="ONT67_002602"
     CDS             complement(2603..3421)
                     /locus_tag="ONT67_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR2356306.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ONT67_002603"
     CDS             3582..4271
                     /locus_tag="ONT67_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR2356307.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ONT67_002604"
     CDS             4285..5787
                     /locus_tag="ONT67_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2356308.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ONT67_002605"
     CDS             5800..6276
                     /locus_tag="ONT67_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR2356309.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ONT67_002606"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ONT67_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAJSBM010000103         6133 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJSBM010000103 DAJSBM010000000
VERSION     DAJSBM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.108"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(207..1169)
                     /locus_tag="ONT67_002607"
     CDS             complement(207..1169)
                     /locus_tag="ONT67_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2356310.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="ONT67_002608"
     CDS             complement(1171..2610)
                     /locus_tag="ONT67_002608"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR2356311.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="ONT67_002609"
     CDS             2795..4741
                     /locus_tag="ONT67_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HCR2356312.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="ONT67_002610"
     CDS             5005..5877
                     /locus_tag="ONT67_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR2356313.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DAJSBM010000104         6001 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJSBM010000104 DAJSBM010000000
VERSION     DAJSBM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.109"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..395
                     /locus_tag="ONT67_002611"
     CDS             <1..395
                     /locus_tag="ONT67_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356314.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="ONT67_002612"
     CDS             398..1228
                     /locus_tag="ONT67_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCR2356315.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="ONT67_002613"
     CDS             1244..2095
                     /locus_tag="ONT67_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356316.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="ONT67_002614"
     CDS             2092..2451
                     /locus_tag="ONT67_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCR2356317.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="ONT67_002615"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="ONT67_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="ONT67_002616"
     CDS             2624..2929
                     /locus_tag="ONT67_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356318.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="ONT67_002617"
     CDS             2929..3240
                     /locus_tag="ONT67_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HCR2356319.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="ONT67_002618"
     CDS             3237..3410
                     /locus_tag="ONT67_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356320.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="ONT67_002619"
     CDS             3407..3823
                     /locus_tag="ONT67_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HCR2356321.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="ONT67_002620"
     CDS             3823..4491
                     /locus_tag="ONT67_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HCR2356322.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="ONT67_002621"
     CDS             4488..4865
                     /locus_tag="ONT67_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356323.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="ONT67_002622"
     CDS             4862..5161
                     /locus_tag="ONT67_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356324.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="ONT67_002623"
     CDS             5151..5309
                     /locus_tag="ONT67_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356325.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="ONT67_002624"
     CDS             5403..5819
                     /locus_tag="ONT67_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HCR2356326.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DAJSBM010000105         5793 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJSBM010000105 DAJSBM010000000
VERSION     DAJSBM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.111"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..988)
                     /locus_tag="ONT67_002625"
     CDS             complement(<1..988)
                     /locus_tag="ONT67_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HCR2356327.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="ONT67_002626"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="ONT67_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2356328.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="ONT67_002627"
     CDS             1813..2967
                     /locus_tag="ONT67_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2356329.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="ONT67_002628"
     CDS             3182..4150
                     /locus_tag="ONT67_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2356330.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="ONT67_002629"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="ONT67_002629"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2356331.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..5788
                     /locus_tag="ONT67_002630"
     CDS             5180..5788
                     /locus_tag="ONT67_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2356332.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1745 a         1131 c         1310 g         1607 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcggcagtg ccattgatct tacgctttat cgattagaca cgggtgagct
     5581 tgtaccaatg gggagccgat ttgattttat ggatgaacgc tctcatcatg cggcaaatgg
     5641 aatatcatgc aatgaagcgc aaaatcgcag acgtttgcgc tccatcatgg aaaacagtgg
     5701 gtttgaagca tatagcctcg aatggtggca ctatgtatta agagacgaac cataccccaa
     5761 tagctatttt gatttccccg ttaaataaac ttt
//
LOCUS       DAJSBM010000106         5790 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJSBM010000106 DAJSBM010000000
VERSION     DAJSBM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.112"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(99..1229)
                     /locus_tag="ONT67_002631"
     CDS             complement(99..1229)
                     /locus_tag="ONT67_002631"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR2356333.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="ONT67_002632"
     CDS             complement(1877..3079)
                     /locus_tag="ONT67_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2356334.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="ONT67_002633"
     CDS             complement(3139..3342)
                     /locus_tag="ONT67_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCR2356335.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="ONT67_002634"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="ONT67_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="ONT67_002635"
     CDS             4879..5691
                     /locus_tag="ONT67_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356336.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DAJSBM010000107         5359 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJSBM010000107 DAJSBM010000000
VERSION     DAJSBM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.113"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            25..696
                     /locus_tag="ONT67_002636"
     CDS             25..696
                     /locus_tag="ONT67_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2356337.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ONT67_002637"
     CDS             755..1195
                     /locus_tag="ONT67_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356338.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ONT67_002638"
     CDS             1207..1875
                     /locus_tag="ONT67_002638"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR2356339.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ONT67_002639"
     CDS             1939..2562
                     /locus_tag="ONT67_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCR2356340.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ONT67_002640"
     CDS             2618..3004
                     /locus_tag="ONT67_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCR2356341.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ONT67_002641"
     CDS             3217..4608
                     /locus_tag="ONT67_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCR2356342.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1778 a          883 c         1124 g         1574 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagtc taatggtgat
     5221 aagtcaagat gaaatttaag attttcttta ttgagtaata tgttttgaag cgcacgaaac
     5281 tagacccgat caaaatcaaa tttttttcgg gttgattcaa ggatttgcgt caataacgga
     5341 ttgttatttc tctatcaca
//
LOCUS       DAJSBM010000108         5268 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJSBM010000108 DAJSBM010000000
VERSION     DAJSBM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.114"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..300)
                     /locus_tag="ONT67_002642"
     CDS             complement(<1..300)
                     /locus_tag="ONT67_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2356343.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNT"
     gene            complement(416..1330)
                     /locus_tag="ONT67_002643"
     CDS             complement(416..1330)
                     /locus_tag="ONT67_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCR2356344.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(1421..1849)
                     /locus_tag="ONT67_002644"
     CDS             complement(1421..1849)
                     /locus_tag="ONT67_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCR2356345.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(1854..2228)
                     /locus_tag="ONT67_002645"
     CDS             complement(1854..2228)
                     /locus_tag="ONT67_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356346.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            2514..2699
                     /locus_tag="ONT67_002646"
     CDS             2514..2699
                     /locus_tag="ONT67_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356347.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            2711..2959
                     /locus_tag="ONT67_002647"
     CDS             2711..2959
                     /locus_tag="ONT67_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356348.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            2990..3169
                     /locus_tag="ONT67_002648"
     CDS             2990..3169
                     /locus_tag="ONT67_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356349.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(3212..3682)
                     /locus_tag="ONT67_002649"
     CDS             complement(3212..3682)
                     /locus_tag="ONT67_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356350.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3769..4467
                     /locus_tag="ONT67_002650"
     CDS             3769..4467
                     /locus_tag="ONT67_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HCR2356351.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            4645..4986
                     /locus_tag="ONT67_002651"
     CDS             4645..4986
                     /locus_tag="ONT67_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356352.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4979..>5268
                     /locus_tag="ONT67_002652"
     CDS             4979..>5268
                     /locus_tag="ONT67_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2356353.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         1753 a          823 c          951 g         1741 t
ORIGIN      
        1 agtgtttaag atattattcg taggataata tgtagactcc ttaacttgtc ttttgtaaat
       61 aactaaccat tcttcgacaa gctcatgaaa tgttagatca ggcttctgaa gtgttttttc
      121 gtttaatttt atatcaattt ttttatttaa ctctagcaat gccactttct gagtttctcg
      181 agatttattt ttataagtaa tgctcacttt ttttcttttt ctagtttttg gatcaatata
      241 tcgctcatta tatttatata ctttttttcc gtttttatca gttttggttt caatccacat
      301 tgttttcatc tcctatttgt tgctataata ggaatggata agtaagccaa ttatagcagg
      361 tttatttttc atcacgtcca caaactttgg cgagggaggg ggcgtgtttt tttgtttagt
      421 agctaccttt ccatgaagta tctgcgaaga acacttcaaa tgttgttcca tcatttttca
      481 cttcaaaata tgctgttcct tgtgcggatt tacccggttg gatggtttcc gaaaagtaat
      541 ctctactagc taaatcagat ttaatatctg atgaatcata aaattctaaa taacttgaac
      601 ttgcatcaaa aggtgtagtc cctgtgtttt ctacaagaaa atctaccttt gcgtaaaata
      661 ggccttctgg tttatagaaa tcatcaccgt aatcttttga tacagactgg acagttacgt
      721 taatagagtt accattttca gaagtaaaag gaactgcttc tcctatacta tgtgcaactg
      781 atgccgtctg gggttctgaa gacgaagttg tagttgtagt tgtagtagag cttgaatctt
      841 tggattttcc atctttatct aagaaagttc gttgattata atatacaacc tgttcagttt
      901 ttggactaac ccaaattaac attgtttcat catcagaatc agttgaataa gtatattgga
      961 gcatctctaa tttttttata tcttttgcac tatttccatg ttcaattaat tcagagtgct
     1021 ctttataata atctccttgt gcatcctttg aaatagatac aattaattca tcccgtgtga
     1081 tagcattgtc taattctttg taaacaaaat catttgtcca tttacgtaac ggtgaaccaa
     1141 gtgtctgata aacttgagtt aaatcatcac caacttttat tttatgaacg tctgatgaag
     1201 ttagtttttt tgtttcatat tcagatgttg atgaaacagt attttcttta gtgctagcag
     1261 aattaggact agtggagcat ccgcttaata ccaagcctaa aaaaagcaac ccaaaaacaa
     1321 tttttttcat ttttaattcc tcacttctat gatatgattt ttttgtagga tcttagaaat
     1381 aaggttttta gtccgtgttg cagcacggac tttttttaca ttatataaga aagcgataag
     1441 cgctttctgg aagtccgtaa agattcttta attcctcaat tcgtttagga tattgatcat
     1501 tgtcttcttt gtatagagaa acaatgagat tagcagcaaa gcaattagct tcgctttcag
     1561 atttgcttct agatgttctt gttgatacat agtaactgga taagccacga tgaaaaatag
     1621 cgtgacctaa ttcgtgagcg caaatgtaga atctttcctc agagtctctc agttcatcat
     1681 ttaagaagat tattgcacga cctctaattt cttgaaactg tcctttggga ttctcgataa
     1741 aaggaacgta ttgaatttta atgcccatct tttcacaaat ataaaaagga ttagcggact
     1801 ggtatttccg cttcaactcc tcgactaaat taatcgtatc catctccata agctcacatc
     1861 tttttgcctt tttctttgtc ttctttcaca atatcccaga aggttccaat aaggatatct
     1921 tttacgcgtt ggacctgttc aggcgtcagt gtttctccgc cataagacat attgacattt
     1981 gaatctagta atttatcaag ttcaactact tcatcttttg ttgcccagtc aggaacttgg
     2041 tttctaccga gtaaataatc agtagttaca ttaaaataat ctgctacaag ttgtagtctt
     2101 ttagtactag gagttctatt tttccattga taaatcgtgt ttttaggtat atttaattct
     2161 tcttccaatt gagttatact tatgcctctt ttatgagcta attcctttat tctatctagt
     2221 aaattcattt tcgcacctca caagctacga aaacactaat aaaaaaatta gttaaaggtg
     2281 ttgacaacta ataaaagtgt tagtatactg ttttcgtaag ctaaaatatt agctaaaaag
     2341 acttgataac taataaacac ttcacggtcg gcaaactctg aaatgtaaat tactaggcat
     2401 ttctgtgtct tatttagcta tgtctatata ctaataaaaa tattagttat tgtcaacgaa
     2461 tattagctaa ttttttagct tacagattat ttttttagaa aggagctatt tttatgtctg
     2521 agaatttaga cttaaaaatt cgagcggaga tgagaaaaag aagaatgact ttcaaagaac
     2581 tagctgcgct tatcggtatt tcaggagctt atttatcaga tattctaaac ggcaatcgtg
     2641 atggaaagaa agcacaacag catatcgaaa cagtgaaaaa aatattggac atccgatagg
     2701 ggggtaggcg atgacaaaac taaaaaaaca agattttgta aaaaaataca attattctcc
     2761 atctacttat caacgtcgaa tgtcggaact aaaaaataca gcaattttct cagcggcgta
     2821 tgaacgggtc acaggacaag aagtttggat caatacagaa ttatacgata aatttttgtc
     2881 tttcaaatcc tataacaggt tacgcacaag aaaggtaaca cctaaagaat ttatcgagaa
     2941 gcatttagtt gatttataaa aaacagaaca ttttgagagg tgaaggttaa tgggtaaatt
     3001 caacagagca ttggtattca gcgcaccgct aatcatctac gctttaggac tttggggaag
     3061 caggcaagcg ttgataggaa cgattgttta catggtttgg atttttatgg ggcttgatga
     3121 agctgaagct gagtacagag cgaaaaagcc aaccgagggg gctgactaaa aatgaataga
     3181 aaagagaaac tagaatgtat attactatta ctcagtttaa ttctttcact aatttctcta
     3241 ttgggaagtt tgtatttttg atatcaacaa ttttgtggat ttgggttcta tttagagtaa
     3301 atatcatttt tgtttggtga ttaaggagtt tattaaaatt aattttccct acttcaaatg
     3361 caaggtaaca agatatagca cttttaggag gaatatttag cggtacagct tcagagtata
     3421 ttctattatc atcgactgat cctcctttag caattaattt cttaaatgga gtagctgacc
     3481 atctgcggtt taagttagcg gatgaaagtt ctaaattaac taacatagct ggttctgttg
     3541 aatagttaga aatgattact tttgtataaa tcatatctgc agcgaagtaa gaagcattaa
     3601 gttcaacttc tagttgaggg cgcattcctt ttatttttat ccctgttaaa aacgtactca
     3661 atataaaact tattattgac atccattgaa aaattgttaa gtgaaaaaaa ttcaaaataa
     3721 acaccaccag tttttaacta aattatacca aaaaggagag aagaaataat gcaagaatta
     3781 gtaattttga aaaataaaga agctgtgact acgagcttac aagtggcaga aagcttcgag
     3841 aaaaaacatc aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt
     3901 ggacagatgt ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc
     3961 ttcattagta gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc
     4021 tacttcaaac aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat
     4081 actgttcctc aaacaattga agatatgatg atctatcaat tagaagaaat gaaagatgtt
     4141 aaaaaagatg tttccatgct taaagatact atgcgaatta gcggacaaca agagtttgaa
     4201 attaagcaaa aaggaaatat gaaagttatg ggagttctag ggggaaaaga aagccgagct
     4261 tatgaagaaa tcagcaaaaa agtattctca aaattttggt ctgaatttaa acgtaccttt
     4321 tcaatcccaa gatatggcga gttacctcgt aagagattcg atgatgctgt ttcatttatt
     4381 gaaatgtggt tgccagaaac tgcgatccgt atggaaattg atcaactgaa cagacaacag
     4441 agactttttg gtgatgacaa tgaatagagc tgaagcgcta agaataggga cggcaattgc
     4501 taattgctgg tggaaatact ataaaccaat catcctaagc caacaacata ttgacaagca
     4561 aaaagcatgg caacaaataa aaaagtgact ccgccggcaa gcaaagagtc acaaagaaaa
     4621 cacatcataa ggagatttta gcatatggaa aaagaacttt ccactctaga tcaatatttg
     4681 attgatcctg attggggcaa gccgaaaatt gaggaaacaa gtggtcgaaa aatcagacga
     4741 aatcttttga cgaatgaaga actagcttgg gatcaagatg atttaggcaa ccatgtaact
     4801 atttgggatc atgtttatct tatccatcta tcgaagcatt cgaataaacc tgaatatatt
     4861 tacgtcatcg aagatggctt gattgatgcg ctagaagagt acgacagaga taacttgatt
     4921 gatatctctt attacggacc aggtaagaaa tacattgctg aaatggaggc agaatttgat
     4981 gagtgaaatc aaagggacaa cgaactttga aaaacttttt agtcgtaagt taaataaaat
     5041 tctcaagaaa aaaggaaatt ttgattattt atcttgggct cacgcgtggg agattatgaa
     5101 aaagaatgat ccacaggcaa cggtaactat taatgagtac aaacactaca gggttgtttc
     5161 tggaactcat caagactttc ttgttgagga atataaacct tttcttatgg atgaaactgg
     5221 gacttatgta tctgtctcag taacggttaa aggacacacg gaaaccga
//
LOCUS       DAJSBM010000109         5212 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJSBM010000109 DAJSBM010000000
VERSION     DAJSBM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.115"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            604..954
                     /locus_tag="ONT67_002653"
                     /pseudo
     CDS             604..954
                     /locus_tag="ONT67_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
     gene            948..1370
                     /locus_tag="ONT67_002654"
     CDS             948..1370
                     /locus_tag="ONT67_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356354.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2540..3508
                     /locus_tag="ONT67_002655"
     CDS             2540..3508
                     /locus_tag="ONT67_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2356355.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            3525..4049
                     /locus_tag="ONT67_002656"
     CDS             3525..4049
                     /locus_tag="ONT67_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2356356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTETHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKTEKKWWQFWQ"
     gene            4425..4706
                     /locus_tag="ONT67_002657"
     CDS             4425..4706
                     /locus_tag="ONT67_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356357.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            4708..5124
                     /locus_tag="ONT67_002658"
     CDS             4708..5124
                     /locus_tag="ONT67_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356358.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1967 a          893 c          868 g         1484 t
ORIGIN      
        1 aaaaaaaaga aaaatccgct tatgcaactt cttctttttg tattttgggg cgctacccca
       61 atccccaagt atcggttatt taatagcgcg gaaatccaaa gaataggacg taccctgaaa
      121 caaaaaaaga ctgctctcac gagcagccca aaatgagatt atcctattca tcaacataaa
      181 aatattacaa agtgaagaac tagagaaaga caaaaaaagc atatcaaatc ctctcaaaaa
      241 taacaagtct taaaaaaatg acagataaaa atgcacaaag aattagatat agataaccgc
      301 gaaaaatcaa gctttagaaa gttgctttta gctagaccaa ttaaaaatca caaagtgagc
      361 gtctctatcc attggtagag gggctacttg gcatagtgta cattatgtta actagctcga
      421 taaataacga ttatgttccc aaaacaatat tttatgaact ataacaaaaa tttgttatcg
      481 catattgtat attttaatta tataattata ggtgtatacc acttaaattt aacacaaagg
      541 cgtggttatt ttgaaaccca aaaagaaaat ttatctaggt ctcctctttt ccttgatgat
      601 tggaatagtc aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat
      661 tactgtggtg tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca
      721 caactaagaa aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac
      781 agccgtcatg cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat
      841 gtgtggaatc cttggtataa ctacccaact gtaataagtg caaatgcagg accagttaca
      901 attcctgtac ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac
      961 ccattgcttt agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac
     1021 aagaaataca aaagactgat ctgattcctt ttctaggaac ttggcaaact aaagatcatt
     1081 cagaacgtct agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct
     1141 tagtaaaaga gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac
     1201 aagttttcat ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat
     1261 tagattacac ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg
     1321 atgggacaga agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg
     1381 tttgatatta caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt
     1441 ggctagcatt tgctagcctt ttttgtattg tggagcgtgg tttttgaggg ggattcaagg
     1501 gggagcttgc gcccctttga caagcggtta tttgcaccaa atacgtaggt atttggctag
     1561 caaatataga gcttgccaaa cactttagtg agttttgtag aaagtacctt taaaggtact
     1621 ttcacattac gaacgatacg tccgtaggac gtactgaaag ggtgggattt atgagcgaac
     1681 taatgcaaaa tttggctagc aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat
     1741 gaacactttt tcaagtgagc gaatccgcca tgacaaagct gaagcagttt atttcagctt
     1801 tgtcatggcg tgtgcctaga cttttaaaaa agtcggcaca cgagcaaata tctttatcaa
     1861 gctctaattt gcgttttaag acgtttgaaa cgtctaagta tataaatata taagagtagg
     1921 tttaagagtg cttaaatcga attttagagg gcattcagac aactttaggg ttgttagggc
     1981 ttgccctgac cgcctgtaag gcgctttaag aacaagataa gggatttata ctaagactgt
     2041 cttaaaacgt ctcaaacgaa ggctcatagc ttctcaaaga gttccttatg aacattttta
     2101 gcaaaggagc cggaagcgac tgcgcagtat aatgtgagag cagagcgatc acaccttttt
     2161 aagcgactgt agggagtaag attcccagtt gagaaaaatg cgcgaatctg tttggagaaa
     2221 aaagaagtgg ttttttaggc gggaaatttt gtccccctct tttataatct ctttttaaac
     2281 ctctttttaa acctctttac cgtcggctat atccttactc tcccaaggga atgacgcttt
     2341 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttatcta catcgttttg
     2401 tctgtttatc tacatcgttt tgtctgtttg tacattgaat tagtacatcg acgatgttaa
     2461 tataagatgt agataaacag gcattttgta tttcataacg tgtatctctt gtgcgagtaa
     2521 gaaaggagct attttaaata tgtctaatga aatcgtaaag tatcatcatg aattaaatac
     2581 aattccgttg cgaaaattta caccagtcga aatgaatttg ttcttttcta tcgtatcaag
     2641 aatgcgtgat gtaggtgata aaaaagtaca atttacattc gagcaattaa aagatttaag
     2701 caattataaa gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa
     2761 attaatggat ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt
     2821 tttatttaat caatttaaaa tcgacggaaa atctgatatt ccttttgctg aaatacaagt
     2881 tcatgaaaaa gccttaccct tacttaataa tcttgaagaa tgggtgcgct attctctcca
     2941 acaattcaat gaattagaaa gcagttattc aaaaacaatg tttcgattac taaaacaata
     3001 cagaaccaaa ggtttcgctt atttttcaaa agaagacttt catgaattac ttgatattcc
     3061 taaaagttac aaccaaggta cgattgatca acgtgtgttc aatccaatac gacaagaact
     3121 aaccgcaatc tttaaaggtt taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc
     3181 tgtcattggc tatcaattta catttaaacc tgagataaaa aaatcagacg actttaacaa
     3241 acatgcccca acaaaagaag aacaaaacaa agcattagaa ctagctaacc aacaagttca
     3301 atctgaaaaa gcacctactt cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga
     3361 acttttacaa cagttagaaa agttagaaag agaacaaaat acccaaaata aagaaaataa
     3421 tgcacaaaaa tcagagataa attccgatat tccttcagaa ctaaaaaaag acttattaga
     3481 caatcttcaa aacttattca aagattgagg ggtttttaga ttttatgaca gaaaaaacaa
     3541 tcaaagaact tgctgaagaa ttaggagtta gtaaaacagc aattaacaaa aaagtttctg
     3601 acgaaaatcg aaaactttgg ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg
     3661 gacaaaaggc tataaaatca atgtttttta acgaaaacga aaactcaaat caacaactag
     3721 ttggcgaaaa aacgaaaacc gaaacccaca aaaacgccaa ccaagttccc gaattagaag
     3781 tcctttctct cctagaacaa cagttaaatc aaaaagatat gcaactacaa gaaaaagacg
     3841 agcaaataaa acaaatgcaa aaattactcg accaccaaca acaactcacc ctgcaagcca
     3901 accaacaaat cgagaaactg caggaacaac tacagttgac ttatacggaa gaatcttcgg
     3961 aaaataaatc aacgactctt tctgaagaaa cagaatcgat agaaaaacaa tcaaagacgg
     4021 agaaaaaatg gtggcaattc tggcaataaa accagcaatt taaattaata aaaattgctg
     4081 gttttattct tctttttgct tttgcgagca gagcaacatg gacccatgtt gcgatatttt
     4141 ggtggcgccc caatccccgt tttttcgttg aatcttactc ggactgcact cgttttacag
     4201 ctgatttttc tgaattttga aagagcataa ggaaacgtgg tatcactttc ttacgaaagg
     4261 tgctagatag gctgtgccta tcacgtttct cagaaaaaaa tttgctatca cttttttgcc
     4321 attgtggcta ggctatgtat agtgttttgg cgaaaaatag ctatacaaat actatatttg
     4381 tatggtatac ataaattata cgctgatacg aaaggaaaat ttccatggac attttagttc
     4441 gaaatatcaa tcccaaaatt attgcaagaa tagataaacg ttgtcaggaa ctcacaacca
     4501 aaacgggcaa aaaatggagt cgaaataaat atctaaaagt tctaatcgaa agtgattttg
     4561 accgtcaact tatgaattac aaaaaagatc aattcgattt attactcgaa aaatttattg
     4621 ccattcaaac agaaaatacg aacatactga atgactatgt tcacacaaac aaccaactca
     4681 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgtaacc
     4741 ctttatccca gtcacgttga cgttttagat tccatcatcg aaaaaaatcc agacgtcaac
     4801 aattattcag aagccattcg ttttttatgt atgagttatg aacctgatac caatacaacc
     4861 acaaccagta accaagcaaa actcaatgcc atgggaaaag aaatttctat catgacagaa
     4921 atcatggcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4981 tctaaagtgt atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga
     5041 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacct ttacttctgg aaattttgac
     5101 ccaacaaact ttctaaaaag ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt
     5161 ctttttaggg atcagtcttc cagcaagatt tgcaagttgg taccaacttg cg
//
LOCUS       DAJSBM010000110         5015 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJSBM010000110 DAJSBM010000000
VERSION     DAJSBM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.116"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            286..1236
                     /locus_tag="ONT67_002659"
     CDS             286..1236
                     /locus_tag="ONT67_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2356359.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            1257..1790
                     /locus_tag="ONT67_002660"
     CDS             1257..1790
                     /locus_tag="ONT67_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2356360.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1806..1976
                     /locus_tag="ONT67_002661"
     CDS             1806..1976
                     /locus_tag="ONT67_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356361.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            2426..2959
                     /locus_tag="ONT67_002662"
     CDS             2426..2959
                     /locus_tag="ONT67_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356362.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2971..3174
                     /locus_tag="ONT67_002663"
     CDS             2971..3174
                     /locus_tag="ONT67_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356363.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            3496..3876
                     /locus_tag="ONT67_002664"
     CDS             3496..3876
                     /locus_tag="ONT67_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2356364.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3858..4772
                     /locus_tag="ONT67_002665"
     CDS             3858..4772
                     /locus_tag="ONT67_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2356365.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
BASE COUNT         1874 a          880 c          917 g         1344 t
ORIGIN      
        1 tttgtcaccc tcttttataa tctcttttta aacctttttt taaacctctt taccgtcggc
       61 tgtgtcctta ctccttcaat ggatacagcg ttttataaac tacattttgt ctgcttttaa
      121 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
      181 ttttaaactc tttttttgca ctcacgtatg ttataatatg cattatcaca gacaaaacgt
      241 tgtttataac gtctgtatcc attgaaggag taaggaggaa tatctttggc aaatgaaatc
      301 gtgaaatacc atcatgaatt aaataccatt ccgttgcgga aatttacacc agttgaaatg
      361 aatctatttt tctctattgt gtctagaatg cgcgaaaaag gcgataaaac agtccgtctt
      421 acttttgacc aactaaaaga tttaagtaat tataaagcaa ctgctaataa tcgtttcatt
      481 aatgatttag aaatgactta tgataaatta atggatttac gttttggtaa aagaagtgct
      541 aatgggctcg atatagatag attcgttatg tttagtgagt ttaaaatacg tggaactgct
      601 aaagatccat atgtagacat acaagtttac gaaaaagctt tacctttact aaacaattta
      661 gatgagtggg ttcgctattc actccaacaa tttaacgaat tacaaagtag ctatgcaaag
      721 actatgtttc gattactaaa acaatatcga actaaaggtt ttgcttattt ttctaaagag
      781 gattttcatg aactattaga tattccaaaa agttactgga acaaccaagc aaacgttgat
      841 aatcgtgtta taaagcctat acgacaggaa ttaaccgcaa tcttcaaagg attatcaatc
      901 aaaaagaaat acggaaaagg gcgaggaaaa cctgtcgttg gctatcaatt tacgtttaaa
      961 ccagaaatca aaaaagcaga tgattttaat aaacatgcac caaccaaaga agaacaaaat
     1021 aaagcattgg ctttagctga taaacaaaaa gaatacgaag aacctgctac aacaattaac
     1081 gaaaaaatca aacagaagaa acgagaactt gaactcctcc aacaaatcaa acaactagaa
     1141 aaagagaaag aagctcttga aaaacaaaca gagtccgcac tttcgaaaaa agagaaaaaa
     1201 agtttattat ctgatttaga taatttattc cagtagcaac ctaggaggca accagtatgc
     1261 aaccacaaga aacctttaca ataaaggaat tgtcagaact ttttaagatg agtaggcaag
     1321 caatatcgaa acatatccaa aaattagatt cttccatgat agctaaaaat gagcgtggtt
     1381 ataaggttgt attgcgttct ggagtattgc aacttgctcg caacttaggc aaccaaatgt
     1441 tgctagatag gttgcacgag tacgaacaag acaacgataa aaatgttaat ataccaagca
     1501 aaaacgtggt tacaaacaag ttgctagatc agttgcgtga agaaaatgaa tttttacgag
     1561 aacaacttgc cctcagagag caaaataatt ctcatataca aaaactactc gaccagcaac
     1621 aacaactcac cctacaagcc aacaaacaaa tcgaaaaact gcaagaaaaa ctacagttga
     1681 cttatacaga agaagctcca gagaatgact caacgactct ttctgaagca acagaaacaa
     1741 tagacaaaca aattgatact gaaaaaaaat ggtggcaact ttggaaataa ctggagggaa
     1801 cattcatgac aagaaaaaat cttctattcg gttctatttt actaggaatt ctaggattca
     1861 gtccgatgtt attgtcagtg atcaataaag ggaataaaca tggtgaattg aaattgaatc
     1921 caaattatta caacagtcct aacgaagaat tgtttgtaaa agaaacagat aaataataaa
     1981 aaagactgct ctttcgagca gccagttaaa aaataatgag tgtttattct tgaaaaagca
     2041 acgcgcttat gaagcgctac caaaaattta tcatatttta ttaaaaataa caacccagta
     2101 aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caaccgcaga aaatcaagct
     2161 taaaaagagc ttttgaccta gaccaatcga aaatcacaaa gtgagcgcct gtatctattg
     2221 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
     2281 tcctaatgca ttctaactat accacattat caaaaatata aattatcttt actaaataaa
     2341 atattgacaa gcctatacct tttgttttat gattttcaca aagaaaacgt tttctatata
     2401 tttttctgta tcaggaggaa attctatgaa aaaatttact ttaggggtag ctttgttaac
     2461 tattggggca gttgttttac cgtcaactac tatagtttat gcttcagaaa attcccaagt
     2521 ggtatcatta aacgaaaaca atgctactga aaatgtagtt attgagtaca ctgaagatgg
     2581 tatggtcgta actaaatatg aggatggtta tagtgcggaa gattttgcaa attcttcaga
     2641 tatcatgaca agagctgcag ttgcaagatg gggcaaaaag gaatatacaa atattgctgt
     2701 aaccactgga gtagctgcta atgctattaa tgctgccttt tgggcaggag ggaaagctag
     2761 cacttctatg tttggtatcc caggttgggc aattggtgct ttgttaacag gtgcaagttg
     2821 gactaaaaaa ggtgcttctc cagggaatgc agttgcaaaa gaatgggaca aaaatggtaa
     2881 tggctggatt ggattttatt accaaagggg atatgatgca gctggaagag ttgtagctac
     2941 tagatattca acgttataag ttaaggagtg atgtttttga tatctaaatc attaaaatca
     3001 ttaaaaacat ttactaaaaa gaatccgcaa atagctacta ttcgtatttt tatatttgct
     3061 atagctgttg ctataattgt taatatgcta agcggagggc aattatacaa atctggttta
     3121 tttttagtgt taatcttagt tgtcttagac ataataaagt ctaaaaaaaa ataaatcgat
     3181 acattttaga agaatagggt tgtttgaata ttacaaagca acaatttcac cttcggtgaa
     3241 ataagcgaaa ctttacaaaa agtggctagc aaatgctagc ctttttttgt attgtggagc
     3301 gtggattttg agggggattc aagggggagc ttgcgctcct ttgacaagcg gttatttgca
     3361 ccaaatacgg aggtatttgg ctagcaaata tagagcttgc caaacccttt tagtgagctt
     3421 tactcattgt acctttttag gtacaatggt attgcgaacg atacgtccgt aggacgtact
     3481 gaaagggtgg gacttatgag cgaacaacat caaaatttgg ctagcgatca ttctaaaaaa
     3541 tatacctatc gatccgagcc aaaacaaatc agttttcgag tgagcgaatc cgaatttgca
     3601 aagctgaagc agtcagctga agctttgcaa atgagtgtgc cggctttcgt gaaagccaag
     3661 gcacaaaacg cgcgtctggt gacaccgaaa gtggcgccag acattgccca ggcaatcgcg
     3721 cgcgatttag ccaaagctgg agggaatatc aatcaaattg ccaagtggtg caatacacac
     3781 caacacgacg ttgctcctga tgatgctcaa cgtttatccg aaaacctaaa aatcatgcaa
     3841 aaggagctac aaaagatatg gcaacagtta aagtaagtcg aaccacttca tgtagccgag
     3901 caatcaatta cgcagaacca cgagcaaccg tcaaaacagg aataaactgt gacattgatt
     3961 atgccaaaag tgaaatgaaa caaatacgca cgctttatgg aaaagacgat cgtgtacaag
     4021 cacacttatt gattcagtcc tttcgaccag gagaaattac cgctgaaaaa gccaatcaac
     4081 taggggcaga gtatgcgaaa aaaatagccc ctaaccacca aatcgcgatt tacacacata
     4141 cagacaaaga ccatattcat aatcacattg tcattaactc cgttaatctt gaaactggga
     4201 aaaaatttca agcccatggg caagcatttt taaacaaatg ttatgaccta aatgacgaga
     4261 tttgtcttga acatgactta acaatcacgg aacgaggaaa gaaacccgaa aaacgaacca
     4321 tgtctgaaat taaattaaaa gaaaaaaatg aaccggtttg gaaagacgaa attcggtttg
     4381 cgattgatca aacaatgaaa aacaaaaaaa ctcgaacgta tacccaattt tgtgattcac
     4441 ttaaaacatt cggcattcat tgcttgaatc gcggaaaaaa ttttacttac gaattggtcc
     4501 acaaaaagaa aaaagtacga tccaacaaac taggaaagga ttacgaaaag gagacgattt
     4561 tacatgagct ggacagacga gaacgaacct acaatcaaca acgaaccaac gagctacgaa
     4621 gagctcgagc tagcattcgc gaaactgcag aagaagtacg aagagaacaa cgaactttta
     4681 cgcctgaaca atcggaaatt agaccgactc gaagaaacca tgaacaacac gttgaacgag
     4741 gaacagacca ccaacaagaa ttatcgctct aaaacgttaa aagacatcga aaacgaaata
     4801 aagagacaac gtgcctttct tgcgccttat acccaagaaa ggatacaaac tacgatggat
     4861 tcacaagtca aaaaactcca ccaagcgcta ttagaccagc gaaaccaaga acaaaaaaag
     4921 gctttaattc aacgatcgat ggatattctt gcccccttat gttgcttagt actaacatta
     4981 ttagcctttt ggttggtatg gggagaataa acaga
//
LOCUS       DAJSBM010000111         4886 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJSBM010000111 DAJSBM010000000
VERSION     DAJSBM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.117"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..284)
                     /locus_tag="ONT67_002666"
     CDS             complement(6..284)
                     /locus_tag="ONT67_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356366.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(244..1158)
                     /locus_tag="ONT67_002667"
     CDS             complement(244..1158)
                     /locus_tag="ONT67_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCR2356367.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(1140..1520)
                     /locus_tag="ONT67_002668"
     CDS             complement(1140..1520)
                     /locus_tag="ONT67_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR2356368.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(1970..2257)
                     /locus_tag="ONT67_002669"
     CDS             complement(1970..2257)
                     /locus_tag="ONT67_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCR2356369.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(2277..2501)
                     /locus_tag="ONT67_002670"
     CDS             complement(2277..2501)
                     /locus_tag="ONT67_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HCR2356370.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(2956..3126)
                     /locus_tag="ONT67_002671"
     CDS             complement(2956..3126)
                     /locus_tag="ONT67_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356371.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(3141..3674)
                     /locus_tag="ONT67_002672"
     CDS             complement(3141..3674)
                     /locus_tag="ONT67_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCR2356372.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(3695..4639)
                     /locus_tag="ONT67_002673"
     CDS             complement(3695..4639)
                     /locus_tag="ONT67_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCR2356373.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
BASE COUNT         1270 a          895 c          831 g         1890 t
ORIGIN      
        1 tctatttatt ctccccatac cagccaaaag gctaacagtg tcatgattaa gcaacataag
       61 ggggcaagaa tatccattga tcgctgcata attacttttt tctgttcttt atttcgctgg
      121 tttaatagcg catggtggaa ttttttgact tgtgaatcaa ttgtagtttg tattctttct
      181 ttggtataag gtgcaagaaa agcacgttgt ttctttattt cattttctat gtcttgtaac
      241 gttttagaac gataattctt gttggtagtc tgctcctctt tcaacgtgtt ggtcatggtt
      301 tcttcgagtc ggtctaattt ccggttgttc agtcgtaaga gttcgttgtt ctcttcgtac
      361 ttcttttgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgttgttg
      421 attgtaggct cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtaatcctt
      481 tcctagtttg ttggatcgta cttttttctt tttgtggatc aattcgtaag taaaattttt
      541 cccacgattc aagcaatgaa tgccgaatgt tttaagagaa tcacaaaatt ggttatacgt
      601 tcgagttttt ttgtttttca ttgtttgatc aatcgcaaac cgaatttcgt ctttccaaac
      661 aggttcattt ttttctttta atttgatttc agacatggtt cgtttttcgg gtttcttccc
      721 tcgttcagtg attgttaagt catgttcaag acaaatctcg tcatttaggt cataacattt
      781 gtttaaaaat gcttgaccat gggcttgaaa tttgttccca gtttccagat tgacggagtt
      841 aatgacaatg tggttatgaa tatggtcttt gtctgtatgt gtgtaaatcg cgatttggtg
      901 gtcaggggct atttttttcg catactctgc ccctagttga ttggcttttt cagcggtaat
      961 ttctcctggt cgaaaagact ggatcaataa gtgtgcttgt acacgatcgt cttttccata
     1021 cagcatgcga atttgtttca tttcgctttt ggcataatca atgtcacagt ttatccctgt
     1081 tttgacggtt gctcgtggtt cggcgtaatt gatcgctcgg ctacaagaag tggttcggct
     1141 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     1201 aagcgttggg cgtcgccagg atcaacgtcg tgttggtgtg tgttgcacca cttggcaatt
     1261 tgattgatat tcccgccagc tttggctaaa tcgcgcgcga ttgcctgagc aatgtctggc
     1321 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     1381 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     1441 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatt gctagccaaa
     1501 tattgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     1561 gcaataccat tgtacctaaa aaggtacaat aagtaaagct cactaaagct cactaaaggg
     1621 cttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa taaccgcttg
     1681 tcaaaggggc gcaagctccc ccttgaatcc ccctcccccc cactctccac aatacaaaaa
     1741 agactagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt cggtgaaatt
     1801 gttgcaaatc aataattagg tagagctaca tttttttcgt tgttcaaaga ttcttatgcc
     1861 atttactatt aataaatcta taaatatgat aatttttcgg ctctataatt tatgggactg
     1921 atgaatcaga attgattctt cagtcccatt ttcattttat tccaaagaat taactccaaa
     1981 taccaataga agcccatcct ctatatactt gagattcctt actaacaatt tcttgtaatt
     2041 ctattaaaga tttaggtaat ccaaatttat gttttaaaat atatcttcca agaaaactac
     2101 ttaatttcat actaacttca ccaacacttt ttccctcact gatttcttta gaataaagaa
     2161 agagcttttc ttttaagtca ggatagtcat ctatagttgc ttcattgtat actttactaa
     2221 ttgcatttaa aagtttttct tctttagtaa aatccattat aataacctcc ctaccactac
     2281 cttctaggtg cccatggacc atgattaacc caaccattaa caataatttt tccaatttct
     2341 cctttagctt gattccaatc tacccaacat ttttctttgt tacaataaag accatttcca
     2401 tagtaagtag ctgcatcaac ttttattcct gtaccgatgc cagctagaca agttcctaat
     2461 attcctagaa taacacaatg ttttaatact ttctttttca tgattgtttt tccttttcgt
     2521 ttttttaaaa ttgcttgtta tattaaataa tcttagtaat tttattatat aacattttat
     2581 tatataacaa tgattaacgt cataaaaagt agttttgaat tcataatcgt ttaaataaaa
     2641 ttataagatc agttatttaa acagctagta gacataatgt gtatactacc aagtaacgcc
     2701 tgtaccaagg gatccaggcg ctgattttgt gaatttttat tggtctaggt taaaagctct
     2761 ttttaaggct tggttttctg cggttgtttc tttctaattc tttgtgaata ttgatcggtc
     2821 attttttaaa tacttgttat ttttgtttgt gtttgttatg ctttttacat atctcatatt
     2881 catttattat tcttttctaa ggttaataat aggataatct ctttcagaaa gactgctcgt
     2941 acgagcagtc ttttcttatt tatcaagttt ttctacaaat acttcctcat tttgactgtt
     3001 gtagtaatta ggattgagtt ttagttcccc ttttttattt cctttgttgg ccactgataa
     3061 taacattggg ctaaagctta atattcctag taggatagaa ccgaatagca agtgtttttt
     3121 tgtcataagt atttccttct tcatttccat agctgccacc attttttcca attctttgtt
     3181 tgttcgtcca ttgtttctgt tgcttcagaa agagtcgttg aactattctc tgaagactct
     3241 tccgtataag tcaactgcag ttgctcctga agtttttcga tttgttggtt ggcttgcagg
     3301 gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg ctctttgagg
     3361 gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa cttgtttgca
     3421 accacgtttt tgtttggtat ataagcattt ttatcgttgt tttgttcatg tttatgcaac
     3481 ttatctagca acctttgatt gtctaagttg cgagcaagtt gcaatactcc agaacgcaat
     3541 acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt
     3601 ttcgatattg cttgcctact catcttaaaa agatctgaca attcctttat tgtaaaggtt
     3661 tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta tctaaatcag
     3721 ataataaact ttttttctct ttttccgaaa gtgcggactc tgtttgtttt tcaagagctt
     3781 ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt ttcttctgtt
     3841 tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta tcagctaaag
     3901 ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg tctgcttttt
     3961 tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt ccttttccgt
     4021 atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt ataggtttta
     4081 taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat aattcaagaa
     4141 agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac agacgaaaca
     4201 tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag cgtacccact
     4261 cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct acgtaaggat
     4321 tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg taagaaagcc
     4381 cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt gcttttaaat
     4441 cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt agttggtcaa
     4501 acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg gaaaaaaaca
     4561 aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca tggtggtatt
     4621 ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat tttttaaatg
     4681 tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac agacaaaatg
     4741 tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataacattct
     4801 gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa gaggtttaaa
     4861 aagagatata aaaagagggg ggctaa
//
LOCUS       DAJSBM010000112         4836 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJSBM010000112 DAJSBM010000000
VERSION     DAJSBM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.118"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(57..173)
                     /locus_tag="ONT67_002674"
     CDS             complement(57..173)
                     /locus_tag="ONT67_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCR2356374.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(418..495)
                     /locus_tag="ONT67_002675"
                     /pseudo
     CDS             complement(418..495)
                     /locus_tag="ONT67_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(649..1284)
                     /locus_tag="ONT67_002676"
     CDS             complement(649..1284)
                     /locus_tag="ONT67_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356375.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            1797..2939
                     /locus_tag="ONT67_002677"
     CDS             1797..2939
                     /locus_tag="ONT67_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356376.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYVCDNPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYEY
                     GYYGYKEHYAYGEIKVMVSDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPTLSEKYKNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDNLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            2932..3138
                     /locus_tag="ONT67_002678"
     CDS             2932..3138
                     /locus_tag="ONT67_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR2356377.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            3217..4410
                     /locus_tag="ONT67_002679"
     CDS             3217..4410
                     /locus_tag="ONT67_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR2356378.1"
                     /translation="MKEKRRDSKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDRLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFQNEVVIIDHQLLKSKEQGYYIETPKTKSGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRGNFLFINHKGKPKVAIDYTMLFVRMVKKYNKNHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
BASE COUNT         1637 a          936 c          922 g         1341 t
ORIGIN      
        1 gcatcaacat gagctaaaac tccaatatta ataattttca tgtgattttc ctccattcaa
       61 aagcccaaaa gggcataaaa atcccagtga taaatactct tatcactggg atttttatgc
      121 ataaccatag gcatacaaag catacagata ttctccggat actttagaat cacatgataa
      181 aggtattctt aaactgggta caaaaaacta agccctccta aaaaaggaca tccaattatt
      241 tgttcccact atcaaattga cagtttattt aagaatacct tgccgcatat ttattaactc
      301 cttttaaata gatacttaaa taatagcacg taagagcata tttgtaaagg aatctccaat
      361 tttttatcaa agagagtacg tgattacaaa atagctgtaa taatgtacca atatttgtta
      421 ttctataatc ttccaattac tcccgttctt ttcaagtacc aaatcaaatt gagatacctg
      481 cgttgccttt gcctgattta ccacaaatca gattttatcc ctttcttcct cttgattgcc
      541 cacaacagaa aagaaaaagc cctgtcaaga gctttgccac cattggtggt gctttgcact
      601 cttgatcggg ctttttgttt gctgtatctt ctcgcaagag gaaaaaagtt actcgtcctc
      661 atcatcaaaa ttgttattcc ttaatacttc tcgtaacagt tccatatctt cttttgagtt
      721 cgggtttatc attttcacga cttgataagg tgtcttatac ttatgtccct ctatgctttc
      781 cccaaacata tccatactgt ataaatcatc atagcggtct aataattttt gaaacttcaa
      841 atgctggata aattctttga ttgggtaaag gtcggacgct tcaatgcttt tcccgttgat
      901 atagtctgta atatattccc gtaacttttc taactcatat tccagccatt cttcctcgct
      961 gtcaaagaag ttatcatagt gtccatgttt cagttctgca tttagacgtt cttcggctct
     1021 taaaaactga cagacttcct tttcagattg attgacatac ttccattctc ccgacaacaa
     1081 ttcattgggc gtaacgtcca gcacttccat tatcttttcc agcgtttcat aggcaggata
     1141 gttcaaccct ctttcaatct tggaaaggct ctgcatattg ataccgattt tgtccgcaag
     1201 ttcctgttgt ttcattcccc taaattttct tatggtctgt atattatttc ctaaatggct
     1261 tattttcatt gcataaccct ccatatctcg ttatatcttc gctattataa gtctatcatg
     1321 cttaatatga tttgtaaagg caaaaaaaga tattgacaaa taagcatata agaattacta
     1381 tgttcttgta atcgaataag cacaatagaa tgaaatataa aaacattcaa aaagaaatac
     1441 ctatctgatt aaatccgtag atatgaatat cggttgaaat ggaacttgtt agaaaaggca
     1501 aaacagaact tgctgacaaa gtatttctga cacggtggcg gaagcctctc aggcttaccg
     1561 ccatgacacc ttgtgtcaag gggaagaaat gctttgtcgg ctggcgagcc tgcgagcctt
     1621 gtctgtgccc ccgttatcta acaggggggc actatttcac aaaagacaag tcaaaaacca
     1681 tacaacccca aagctgtacg gagtaatgag gagttttaag aagatagtac acttcacact
     1741 tttttgaaag gcggtttaca ggtatttcaa cgctattgtg ggattggagg aatacaatga
     1801 acaacttaga atttgcactt gaaatgaaga acaaacgtct tgccagcggt ctttcacaag
     1861 gagaacttgc aagtcttacc catgtatcaa gatatagtat caaccgcttt gagaacggaa
     1921 aagccaacgc cagcaaggaa acacaaaata ttatcctgcg ttgtctgaat tactatgtct
     1981 gtgataaccc tttttatctg ctggtggatt atctgtctgt ccgtttccca acgactgacg
     2041 cattggaagt tatccgaaag gtgcttggca tgaaagcaga ttattttatc cattatgaat
     2101 atgggtatta tggctataag gagcattatg cctacggaga aatcaaggtt atggtttctg
     2161 atgatgaaca catgggcgta tttttggaat taaaaggggc aggctcacgc aacatggaat
     2221 acgtcttaca ggcacagaac agggattggt attcattctt aaaccgctgt cttgactgtg
     2281 gcggtgttat ccgacgtttt gacttggcaa tcaacgatat gtgcggtctg ctggatattc
     2341 ccaccttgtc tgaaaaatac aaaaatggcg gtgcggaatg tcgctgtaaa aactatgaaa
     2401 atgtgcaggg tggaaaacta agcggaaaaa accgtaattt agcaagcact ctttatatcg
     2461 gctcaaaggc aagcacaaaa tatttctgcc tgtatgaaaa acaaaaggaa caggcaacta
     2521 aaaagaaaca tacggatatt atcaaccgtt ttgaaattcg tctgcgtgac aaaaaggcag
     2581 tacaggcagt tgaagaactg ttattgacgt ataaccctca tgggctggtg ttttacctca
     2641 ttaccgattt tgtggaattt cccgactatc cattgtggga gatattcatt tcccatgata
     2701 atttaccttt tgaaatgaac cctgtacctg tcaatatgga acgcactctg caatggctgg
     2761 aaagacaggt catgccgtcc attgtgatga tagaagaaat cgacaggctg acaggttcaa
     2821 actatatgaa aatgattgat gaatgtaccc acctttccga aaaacaggaa atgcttgtgg
     2881 agcagatgtg tacggacata gcagacgtaa tcgaaagtga gggggtgttt tatgagtaat
     2941 gcacaggata ttcccgtatg ggaaaaatac acccttacca ttgaagaagc gtcaaagtat
     3001 ttccgtatcg gagaaaacaa gttaagacgc ttagcagagg aaaacaagga cgctggctgg
     3061 ctcattatga atggcaaccg catacagatt aaacgccgac agtttgaaaa ggtcattgat
     3121 aaattggacg caatctaatg caaatgagcc ttgtatgtgt tatgatgaac acaagtcata
     3181 tcaaggctct ttccaacaag gaaaggagca gacaccatga aagaaaaaag acgggatagc
     3241 aaaggacgta tcctgcatac tggagagagc caacgaacag acggaaaata cttatataaa
     3301 tatgtggacg catttggaaa cacaaaatat gtgtatgctt ggagattgac acccacagac
     3361 ccgacaccaa aaggaaaacg ggaaaaaccc tcacttcgtg aactggaaca gcagataaga
     3421 cgggatattg aggacggtat cgacagcaca ggcaagaaaa tgacgctttg ccaactttat
     3481 gccaaacaga acgcacagag ggcaaacgtg aagaaaagca cacagaaaca acgggaacag
     3541 ctcatgcgat tactaaaaga ggacaggtta ggtgctagga gcattgatac gataaaaccc
     3601 tctgacgcta aggaatgggc gttacgcatg aaagataaag gcttttccta taacaccatt
     3661 aacaaccata aacgctcgtt aaaagcgtcg ttctatatcg ccatacaaga cgattgtgta
     3721 aggaaaaacc cttttgattt caagttaagt gaagtcctag aaaatgatac caaagagaaa
     3781 gtcgcattga cagaagaaca ggaacaagcc ctactctcat tcatcaagac ggacaatgtg
     3841 tatcacaagt attatgatga tgtgctgata ctgttaaaga caggacttcg tatctcggaa
     3901 ctgtgcggac tgacagtagc cgatattgat ttccaaaatg aagttgtgat tatcgaccac
     3961 cagttattaa aaagcaagga acagggttat tacattgaaa cgcctaagac aaaaagcgga
     4021 acaaggcaag tgccattaag cagagaaacg atacaggcat ttcaacgggt tatgaagaaa
     4081 cgcccaaagg cagaaccatt tgtgatagac ggacggggca actttctatt tatcaatcat
     4141 aaaggcaagc ccaaagttgc cattgattac actatgcttt ttgtccgtat ggtaaaaaaa
     4201 tacaataaga accataagga caacccattg ccacatatca caccgcatac actacgccat
     4261 acgttctgca caaggctggc aagcaagaac atgaacccga aagatttaca gtatatcatg
     4321 ggacattcaa atatcagtat cacaatgaac tggtatgccc atgcgtccat agataccgca
     4381 aaatcagagg ttcagcgtct aatcgcataa gaagtattta ccacgatttt aaccacgatt
     4441 gacagggaaa atataagaag atagacctag atatgtgagg tttaccacaa aagcaaaatg
     4501 cccgtagagc cgataaaata aggctttacg gacatttaag aagatataaa aagatagtca
     4561 aaaagacata tataatttat acctaattta agtaagtaca aacatcattt taagaatagg
     4621 aaaggacatt tatatactat tctctgtatt tttgatttat aaataaatat gaattgaatc
     4681 caaatcagct atattactat tttataataa ccaactataa aaaacgaagt taggtgaaaa
     4741 actaacttcg atattcataa aagactctat tagttcaact tagcaaaaga tttaaacaaa
     4801 aaaagatgaa ctatttctag ttcatctttt tgcctt
//
LOCUS       DAJSBM010000113         4832 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJSBM010000113 DAJSBM010000000
VERSION     DAJSBM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.119"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1131)
                     /locus_tag="ONT67_002680"
     CDS             complement(<1..1131)
                     /locus_tag="ONT67_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2356379.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDD"
     gene            complement(1261..2637)
                     /locus_tag="ONT67_002681"
     CDS             complement(1261..2637)
                     /locus_tag="ONT67_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2356380.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2637..3293)
                     /locus_tag="ONT67_002682"
     CDS             complement(2637..3293)
                     /locus_tag="ONT67_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR2356381.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3303..4580)
                     /locus_tag="ONT67_002683"
     CDS             complement(3303..4580)
                     /locus_tag="ONT67_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR2356382.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4599..4760)
                     /locus_tag="ONT67_002684"
     CDS             complement(4599..4760)
                     /locus_tag="ONT67_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR2356383.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT         1520 a          877 c          587 g         1848 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
       61 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      121 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      181 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      241 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      301 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      361 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      421 aatgaaaaaa acatcttcta caccacctac tttcagttca tcaaatactt gcatccagcg
      481 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
      541 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
      601 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
      661 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
      721 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
      781 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
      841 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
      901 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
      961 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1021 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1081 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1141 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1201 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1261 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1321 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1381 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1441 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     1501 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     1561 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     1621 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     1681 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     1741 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     1801 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     1861 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     1921 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     1981 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2041 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2101 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2161 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2221 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2281 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2341 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2401 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     2461 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     2521 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     2581 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     2641 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     2701 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     2761 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     2821 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     2881 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     2941 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatct aatattttca
     3001 attggtgata aataatctat taaattatag ttttgaaata ctaaagatat attatttttt
     3061 ctatgattac tatatccttt attttgtata tcttcattct taaacaatat ttttcctgtt
     3121 tgaggtttat caagtccagc aagtaaggaa agaagtgtag attttcctgt tcctgacttt
     3181 cctactatcg cataaaactt cccaagttca aatttttgat ttactcctga caaaactttt
     3241 tctttagaat ttgcataact atatgctaca ttctttattt ctaatatatc cattattttc
     3301 tcctaactta tttttgataa gatttcttta ggcttcttga ttaacattaa tgaagaagca
     3361 aatacaactg ataaaacaat aatacttatt aatattaaat aactttgtcc aagtgttgtt
     3421 atatttgaca taaaactact attatttatt aaacttccac cggaaatcat tgaatcctct
     3481 gagttaatga atccatctac aattactttt agtaatacat ttcctaaaaa taaggaagat
     3541 attatacttg gtatcgatat gaatattaac tcgaatataa attgcattat aatttgtatc
     3601 ttagatgttc caatagataa gaatatacct atttcataaa ttctttccct taaccataga
     3661 atcaagatta atgaaagaac aaccatccca cctaacataa tagaataagt cattactttt
     3721 attatatatt ttattccact cactgactct aaagactctt caaacgcatt agaatctttt
     3781 tcaacaaaat actttgactc atcaatttta agctctttca atttgtttaa ggctaagtct
     3841 gtagattctg cactaccaga atacattaaa attttatttg caatttcatt attttctgaa
     3901 ttatttaata tctcttggct tgttgaataa tctacaaaca ccatattttc actaaaatca
     3961 gaagataatc ctgtatatgt ttcctgtttt ttaccagaaa agatccctat aattttaaat
     4021 ttatgacttt ttatttttcc acttttttca atatctagta attcaagatc aacttcatca
     4081 cctaatttta gattgttttg tttagcaaat tcttcatgaa caataattga attcttatca
     4141 ttttcttcta tatttttacc ttctttaatt gtaaatactc cactactaaa taaaatattt
     4201 cttttagtat tatttgtagc ttcgagtgaa acaacattct taaattcgtc agataaatct
     4261 tctctattta ttctttgttc tccgctaact actttagcat cttttagttt tgctaatcca
     4321 tcatattgaa ttattttttc ttcaatttct tttaattttt caatatcttt aaattgatta
     4381 acattaaaat atttaccatc tttttttgtt attgatattg aagaattaga actttcatat
     4441 aaagcctttt ctatttcatt gcttgatttc attattgtta aacaagaata caagcaagaa
     4501 agaactatag ttaatatgat aaaaattatt aaagttctat ttttctttct tgtaatatat
     4561 gctatagcat tttttatcac tttcctaact actcctctct attttcttac tccataacta
     4621 attgcatcca ctggacaagc atttttacat cgtccacatc tgatacactc cagatgattg
     4681 caattctcaa ctgaatcttt attcatttgg caaacttttt tacattttcc acaattaata
     4741 cacttatcct tatcctgaat cggctacact agactagaca gaaaaaataa ggtgtgtaga
     4801 ataataaaaa acacactgga ggatttttca tg
//
LOCUS       DAJSBM010000114         4828 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJSBM010000114 DAJSBM010000000
VERSION     DAJSBM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.120"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT67_002685"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="ONT67_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCR2356384.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="ONT67_002686"
     CDS             complement(3004..3438)
                     /locus_tag="ONT67_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356385.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="ONT67_002687"
     CDS             complement(3455..4660)
                     /locus_tag="ONT67_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCR2356386.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1506 a          900 c          649 g         1773 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatca
//
LOCUS       DAJSBM010000115         4782 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJSBM010000115 DAJSBM010000000
VERSION     DAJSBM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.121"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..258)
                     /locus_tag="ONT67_002688"
     CDS             complement(<1..258)
                     /locus_tag="ONT67_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356387.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="ONT67_002689"
     CDS             complement(274..924)
                     /locus_tag="ONT67_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356388.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="ONT67_002690"
     CDS             complement(935..2107)
                     /locus_tag="ONT67_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCR2356389.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="ONT67_002691"
     CDS             complement(2126..2998)
                     /locus_tag="ONT67_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2356390.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="ONT67_002692"
     CDS             complement(3020..3940)
                     /locus_tag="ONT67_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356391.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="ONT67_002693"
     CDS             complement(3940..4209)
                     /locus_tag="ONT67_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356392.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="ONT67_002694"
     CDS             complement(4206..4586)
                     /locus_tag="ONT67_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR2356393.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1253 a         1050 c          789 g         1690 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ct
//
LOCUS       DAJSBM010000116         4710 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJSBM010000116 DAJSBM010000000
VERSION     DAJSBM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.122"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            106..357
                     /locus_tag="ONT67_002695"
                     /pseudo
     CDS             106..357
                     /locus_tag="ONT67_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="ONT67_002696"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="ONT67_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2356394.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="ONT67_002697"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="ONT67_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="ONT67_002698"
     CDS             1786..1869
                     /locus_tag="ONT67_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2356395.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT67_002699"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="ONT67_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2356396.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="ONT67_002700"
     CDS             complement(2994..3857)
                     /locus_tag="ONT67_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HCR2356397.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="ONT67_002701"
     CDS             complement(3859..4131)
                     /locus_tag="ONT67_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2356398.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="ONT67_002702"
     CDS             complement(4148..4357)
                     /locus_tag="ONT67_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCR2356399.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="ONT67_002703"
     CDS             complement(4455..>4710)
                     /locus_tag="ONT67_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCR2356400.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DAJSBM010000117         4632 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJSBM010000117 DAJSBM010000000
VERSION     DAJSBM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.123"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            174..518
                     /locus_tag="ONT67_002704"
     CDS             174..518
                     /locus_tag="ONT67_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR2356401.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="ONT67_002705"
     CDS             519..938
                     /locus_tag="ONT67_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR2356402.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="ONT67_002706"
     CDS             complement(1030..1380)
                     /locus_tag="ONT67_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356403.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="ONT67_002707"
     CDS             complement(1346..2566)
                     /locus_tag="ONT67_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCR2356404.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="ONT67_002708"
     CDS             2932..3498
                     /locus_tag="ONT67_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR2356405.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="ONT67_002709"
     tRNA            3643..3730
                     /locus_tag="ONT67_002709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="ONT67_002710"
     CDS             4064..>4632
                     /locus_tag="ONT67_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCR2356406.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DAJSBM010000118         4608 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJSBM010000118 DAJSBM010000000
VERSION     DAJSBM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.124"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(83..337)
                     /locus_tag="ONT67_002711"
     CDS             complement(83..337)
                     /locus_tag="ONT67_002711"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2356407.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(448..702)
                     /locus_tag="ONT67_002712"
     CDS             complement(448..702)
                     /locus_tag="ONT67_002712"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356408.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(895..1170)
                     /locus_tag="ONT67_002713"
     CDS             complement(895..1170)
                     /locus_tag="ONT67_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCR2356409.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1142..2095)
                     /locus_tag="ONT67_002714"
     CDS             complement(1142..2095)
                     /locus_tag="ONT67_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2356410.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2707..4200
                     /locus_tag="ONT67_002715"
     CDS             2707..4200
                     /locus_tag="ONT67_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCR2356411.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4335..>4608
                     /locus_tag="ONT67_002716"
     CDS             4335..>4608
                     /locus_tag="ONT67_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCR2356412.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          801 g         1541 t
ORIGIN      
        1 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
       61 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccatga
      121 tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt aaatttaatt
      181 gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt aataaaactt
      241 gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct
      301 ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta taaaaataat
      361 agacacttct agggatttct aataaccgac acaacaaaga aacagtgtaa tccttacacc
      421 atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg ctgcggcttt
      481 ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt cagctacttc
      541 ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt tgtaaatcgt
      601 tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag tttgattcag
      661 ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc atcctctcgt
      721 agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga tactaacacc
      781 aaatgttgta tacccccaaa caatacacat attttgaagg attaggatta ataaagctaa
      841 aaatatagta agtatactaa atatatttac tatatctagt atacttacta tattttaatc
      901 ttttgatcgt ctattatata taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc
      961 cattgcaagt ttgactattt catacatagg aatatctttt gcataactaa gatcccgaat
     1021 ggtttttagt acgggaggat ctacttgaac tggttttctt tctttcgcag tgtactttcg
     1081 ttcccctaac tcgacattaa tatcatcttt aattgaaata tcttcagcac caattttctt
     1141 cttatctttg ttcgttggta ataatgccat ttagtttaat ctcctttcca agaggtagga
     1201 tatgattggc cattgagtca gcatatttag gtgtataatc aaatccttca atatcaccat
     1261 gttgttcata gtattcaatt ctttcttcta attctgtaaa gatgtctgca aaaatagccc
     1321 accatcttcg atctacataa tcatttaatt ggataccatt ctcaccatac atttctaacc
     1381 gatccgagcc tttaacaatg cttttaaaaa tgtttgattc accatactta tcaattgttt
     1441 tttgatagtt tgcgtgttga gaggaacgtc gcactgagaa aagaacaggc aaaattccaa
     1501 tgatttgaag atgagactct tcaaaattcg tgacaatcgg aactaaaact ttattaataa
     1561 aatcttctgt tccttccatt gcgtaccgtt ttaattcttg cattgcaata atataatctg
     1621 cagcagttaa gaatgcacga accacattat ctgttgatgg tgggcagtcg ataaagatat
     1681 aatcgaaatt ttttctcaat tcttctaaag gtttaatgaa ggccaatgat ctcttacgac
     1741 gatcaggata tttttcggac aagtattcat taagttcttc agatccagga gagccagcca
     1801 taatatataa atgtggactt acttgagtaa tacctgaaac ccagtcccct tttttgattg
     1861 ctgcagacat ggatcgatca aagctagtaa tttgatacgt tgatccaatg ttggaagtag
     1921 tatttctttg ttggtcataa tcaattagca acacgtcttt ttcaaaaagt tgactagaaa
     1981 caatcgctaa taaatcattg tctgtagtct taccaatgcc gccttttcta cctgtaagca
     2041 ttacaacttt tcctagttta tctgatgttt taaatgcagt ccgtaagttt tccatagttc
     2101 cctccaattt cctatactaa atatatttag tatacttcat atacttaata tattcgatat
     2161 atctaatata ctccaaattg ataagaattc agactttata aggaatatag tggatatact
     2221 aaatatattt gctatattca gtatatcaga tatattcaaa atgtcaaaat atatttgtgc
     2281 gttttgcgtt gacaatttag agtgaaaaga ggtaaactta attctataga aaccacagat
     2341 aattcactat ttacaaagta gcaaaagcag tggtactctt taaacagaaa aattacataa
     2401 aaaaaagaga cggaaataat tgattttagc agaatcaatt atttctgaat cacatggtta
     2461 ggacatgtgt tcacgctcgt ctcaagaata tgtaagtagc taccaactac ttatattaaa
     2521 agttgatcgg ataccttttc cgattgcctt tattctacca aaaaaaggcg agcaactcaa
     2581 cagaagaacg tttatttagt gtacttaaat taaatattct taaacactga agagagaaca
     2641 catactatgg ctgagattaa agctaaactg tgttctcttt ttgtttgaga aaggacgtta
     2701 caatcgatgt tgagcgaaat ttattcagct attcttaaaa acggcttacg tacatacaaa
     2761 tatcgaaaca gcaccatgaa acctgcaggt catattgatt ccagtaggag tggtgctatc
     2821 tttgcgtacc gtagcaagca attaatgaat gttgggcgtg gtatggtcat tacttcggaa
     2881 gaagctattt tagagaacga aaagaaacta acgcattgga cacctaatac ctatcgcttt
     2941 ggtacttatg cggatgatta tcgaacagtg gttaaaggtc attctgagaa aaacttaagt
     3001 caaatcaaca catttgtagt tgatatcgat tcaaaagaga accatcaagg cgaaattatt
     3061 ttagcctgtt tagatcaagt gggctacatg ccaacgctaa ttttagaatc agatcatggt
     3121 tatcaagttt attttgtact aaagacacca ggctatgtca caaagaaaag caatttcaag
     3181 gtgatcgatg tagcaaaaaa aatttcaaaa acaattcgtc tacagttggc tgaaaaattg
     3241 tccggagtgg atctaggatg taaccacttc ggaattgctc gattcccaca agaaaataat
     3301 attgttttta aagaaatgaa ctatcagtat tcttttgctg actggattca atggtcgatg
     3361 aagcttgaat cagatcaaaa aacaaaagaa gatcgagaac gaaaactaat tgtctttcct
     3421 gagaaaaaag aaattcgtca agtagatgaa ccgtggttcg atatgttact tcataaagca
     3481 aacatcgtcg gcggagaagg acgtcttggt cgaaataatg tgattttcac gctttcttta
     3541 gcttattact cttcaggtta tgcattggat acttgcgaat acaatatgtt cacattcaac
     3601 gaacggttac aggaaccgct atctgaagca gaattagtaa agattgtacg tagtgcttat
     3661 tctggtaatt atcaagcagc aagcagagaa ttcattattg aattgtgcca gcaatgggtt
     3721 tcaccaacct taaaaaataa tgatctgttc attcaacgac gtggctggtg gaaatttaaa
     3781 aagccacgag atcaaagaaa atatagtcat aaaaatgaat ggaaagaaga tctattggct
     3841 tatctaagcg aaaaaagtct tgctagtgag ccttatttga acctgtcaaa aaaagaattg
     3901 gcttcacaac taacgatgcc caagcgaaca cttgataaga tcttaaatga gttacgagag
     3961 gagaataaag tcttctacaa ggtaacacga ggaagaaatg gcggaatgat cattgcttca
     4021 gtcaaaacgt tgttcgcgag ggttattcaa ctaagtaaag aaaagagact agattattca
     4081 acaaggatca cagaggtatt tggactcaac gagagcttag tagaagagac atttagacaa
     4141 ttatcaaccc ccttgaaccc tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga
     4201 aaaataaaag gtgcaaattg ccttatacaa tatatctatg atacttctgt aggtgttgct
     4261 ccctttgcct agctacgtgt aggtggtata cttataactg cgtatgcagt cttatgtatc
     4321 atggaggaag ttgtatgtcg ttaaaaaatt atgtctattc acatcctgtt aatgtgttta
     4381 tcattggtcg tttgggatta tctgtggaag tgttctgtga gctttatggt tttaagcagg
     4441 ggactttgtc ttcttgggtt accagggaaa aaacagttgc ttctttgccg attgagtttc
     4501 ttcatgggtt gtccttagct tctggggaga ctatggatca ggtatatgat tgtttgtgtg
     4561 tgttagagca ggagtatatt gagtataaaa tagctaacga gttaagaa
//
LOCUS       DAJSBM010000119         4470 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJSBM010000119 DAJSBM010000000
VERSION     DAJSBM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.125"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            318..1049
                     /locus_tag="ONT67_002717"
     CDS             318..1049
                     /locus_tag="ONT67_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCR2356413.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="ONT67_002718"
     CDS             1194..3221
                     /locus_tag="ONT67_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCR2356414.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="ONT67_002719"
     CDS             3329..4426
                     /locus_tag="ONT67_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCR2356415.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          923 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt
//
LOCUS       DAJSBM010000120         4433 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJSBM010000120 DAJSBM010000000
VERSION     DAJSBM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.126"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(543..2048)
                     /locus_tag="ONT67_002720"
     CDS             complement(543..2048)
                     /locus_tag="ONT67_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCR2356416.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ONT67_002721"
     CDS             complement(2045..2731)
                     /locus_tag="ONT67_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2356417.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT67_002722"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ONT67_002722"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR2356418.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DAJSBM010000121         4425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJSBM010000121 DAJSBM010000000
VERSION     DAJSBM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.127"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            690..1658
                     /locus_tag="ONT67_002723"
     CDS             690..1658
                     /locus_tag="ONT67_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2356419.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="ONT67_002724"
     CDS             1669..2484
                     /locus_tag="ONT67_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR2356420.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ONT67_002725"
     CDS             2552..3262
                     /locus_tag="ONT67_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR2356421.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ONT67_002726"
     CDS             3298..>4425
                     /locus_tag="ONT67_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2356422.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAJSBM010000122         4345 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJSBM010000122 DAJSBM010000000
VERSION     DAJSBM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.128"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(387..1853)
                     /locus_tag="ONT67_002727"
     CDS             complement(387..1853)
                     /locus_tag="ONT67_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR2356423.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT67_002728"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="ONT67_002728"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCR2356424.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DAJSBM010000123         4310 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJSBM010000123 DAJSBM010000000
VERSION     DAJSBM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.129"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(65..1285)
                     /locus_tag="ONT67_002729"
     CDS             complement(65..1285)
                     /locus_tag="ONT67_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCR2356425.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="ONT67_002730"
     CDS             complement(1305..1916)
                     /locus_tag="ONT67_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR2356426.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="ONT67_002731"
     CDS             complement(1959..2891)
                     /locus_tag="ONT67_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCR2356427.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="ONT67_002732"
     CDS             complement(3312..4172)
                     /locus_tag="ONT67_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356428.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DAJSBM010000124         4203 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJSBM010000124 DAJSBM010000000
VERSION     DAJSBM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.130"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..354)
                     /locus_tag="ONT67_002733"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="ONT67_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="ONT67_002734"
     CDS             complement(614..1162)
                     /locus_tag="ONT67_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356429.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="ONT67_002735"
     CDS             complement(1163..2020)
                     /locus_tag="ONT67_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCR2356430.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="ONT67_002736"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="ONT67_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="ONT67_002737"
     CDS             complement(2582..2911)
                     /locus_tag="ONT67_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2356431.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="ONT67_002738"
     CDS             complement(2988..3260)
                     /locus_tag="ONT67_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2356432.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="ONT67_002739"
     CDS             complement(3260..3523)
                     /locus_tag="ONT67_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2356433.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="ONT67_002740"
     CDS             complement(3629..4045)
                     /locus_tag="ONT67_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356434.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="ONT67_002741"
     CDS             complement(4051..>4203)
                     /locus_tag="ONT67_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2356435.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DAJSBM010000125         4194 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJSBM010000125 DAJSBM010000000
VERSION     DAJSBM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.131"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1008
                     /locus_tag="ONT67_002742"
     CDS             <1..1008
                     /locus_tag="ONT67_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356436.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="ONT67_002743"
     CDS             1025..1174
                     /locus_tag="ONT67_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356437.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="ONT67_002744"
     CDS             1171..1617
                     /locus_tag="ONT67_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356438.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="ONT67_002745"
     CDS             1619..1756
                     /locus_tag="ONT67_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2356439.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="ONT67_002746"
     CDS             1794..2087
                     /locus_tag="ONT67_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356440.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="ONT67_002747"
     CDS             2084..2308
                     /locus_tag="ONT67_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2356441.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="ONT67_002748"
     CDS             2305..3330
                     /locus_tag="ONT67_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCR2356442.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="ONT67_002749"
     tRNA            3520..3593
                     /locus_tag="ONT67_002749"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1450 a          719 c          842 g         1183 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtc
//
LOCUS       DAJSBM010000126         4180 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJSBM010000126 DAJSBM010000000
VERSION     DAJSBM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.132"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            176..1264
                     /locus_tag="ONT67_002750"
     CDS             176..1264
                     /locus_tag="ONT67_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356443.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1261..2682
                     /locus_tag="ONT67_002751"
     CDS             1261..2682
                     /locus_tag="ONT67_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCR2356444.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2718..3434
                     /locus_tag="ONT67_002752"
     CDS             2718..3434
                     /locus_tag="ONT67_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2356445.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            3453..>4180
                     /locus_tag="ONT67_002753"
     CDS             3453..>4180
                     /locus_tag="ONT67_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HCR2356446.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEY"
BASE COUNT         1549 a          487 c          756 g         1388 t
ORIGIN      
        1 atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg atagtttcgt
       61 gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt aattatgaat
      121 ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa aaaatttgaa
      181 aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac aaatgaaaaa
      241 tacaattttt tccgatgagt atgttgaaat tattatttct gatcattcta aaaacactaa
      301 agaaattgcg aaaaaaatag aagaaaaaaa agtgtttgat ggctgtcatt atttagaaag
      361 caaatctttt gatgataata ttgggtattt tcaaaagatt aaggactgtc ttcagatttc
      421 ttgtggtaaa tcaaatcgat atgcctatta tttgaaaaac tgtaaaagtg aatattttga
      481 tgaatttatt tgtttttcaa tgggagaggt ttggttggaa ggaatatatg cttgcttata
      541 tcgaaataat aaaaatgtac aggtatcaat tttagaagaa ggtattttaa gctatggagt
      601 tgacattatt ccaacagcaa gaacaaaagc tatcgcagta ctcaattact taaaaaaaag
      661 gactccacta tataacaaaa gctataattt ttattgtttt tttcctaata tatatacagg
      721 aagagagaat acaatacaaa ttccattagt tacagctggg tcaagaaccg cattgcaact
      781 ttcggatatt tttaacccta tgatagatat ttataaagaa aaaaaatata tattttttac
      841 gtctgtttat gattttgagg gcggagaacc tgtcggagaa tatgaattag tatgcaaaat
      901 agcagactta gtaggaaaag ataatctaat tgttaagacg catcctagag atgtaagaac
      961 aatttataaa gataatggat ttatcgagga taaaaattca tcaatacctt gggaggcgat
     1021 tcaattgtca ggcgatttta acgataaaat atttatgaca attaattcag gaagtgttat
     1081 ttcaggtagt acaatgtctg caaaacctgt acgcacgtat tatatgtata aattgtgtaa
     1141 catagaagga aatccgtctt gcaaaaagaa tgcgtatgat attgagcgat tgcttaagca
     1201 aaaaacgatg aaaaacattt taaagaatgt gaaaatagca gaaagcttag atgatatact
     1261 atgagcaaaa ctaaatattt aattaaaaat atgggaatat taacaataag taatttttct
     1321 tcaaaaattt tagttttttt gttagtccct ttatatacaa gtgtattatc cacaacagaa
     1381 tatggcacat atgatttgac aatttcaaca gccacattat tgtttccaat tttaacctta
     1441 aacattgtag atgctgtgat gcgcttttta atggataaag ataaaaataa aaaaaatata
     1501 gcatctatag cgattaaagt tatattttta agcgtgatag tctttggatc cgtaatgtat
     1561 ttgttaaata ttacaaaatt atggcaagat gtttatggat tagaaaaata tatttttttg
     1621 tattatatat cctatgtatg gaatcaattt ctaatacaat ttgctaaagg gttagaaaaa
     1681 gttaaagcta tgggcatagc aggtattatc agcacaattg taacaataat tgccaatatt
     1741 ttcttcttgc ttattctgaa atggggatta attggttttt tcattgcaaa tattttatca
     1801 cagattattt cagctactta tctgtcagta ttagtgagaa tatgggaata tatcgatttt
     1861 gaggctaaag atcctcagct aaagagagaa atgctaaatt actcgattcc attaattgca
     1921 actgttgttg gatggtggat taatagtaca gctgataggt atgtggtttc ctttatagta
     1981 ggtgtctcgg cgaatggctt attgtctgtg gcatataaaa tacctcaaat aattaatact
     2041 ttgcagggaa tatttataca ggcctggcaa atttctgcaa ttaaagaata tggagataat
     2101 gagactgcaa aattttatgg aaatacattt gttatgatta atttgttaat gtgcataacc
     2161 tgttcaagtc ttatcatatt aactagaccg ttttcacata ttctttatgc gaaagacttt
     2221 tatgaagcgt ggaaatatgt tccattttta ttaatagcta atgttttaaa ttgtgcatca
     2281 ggtctgttag gacctatctt atcagcaaaa aaagattcta aatccatgat gtggtctgca
     2341 atcatagggg cattagttaa tgtgattatg agtgtagtcc ttgtttataa gataggggtt
     2401 caaggtgcca caatagccac tgttatatgt tcatatgtaa tatatgttgt tcgtaaacga
     2461 gctgtgggaa aagatatttt tataaagcat tatcatattg ttgtggaatc ttggggatta
     2521 ttgtttgttc aggcaatcat agaaatatat tttaaaaatt atgtaataga aattataatt
     2581 atgattattt taattattat aaatatcagt tatattaagc agttgtgtta tagaggaaga
     2641 aacattattt caatgattaa gagtcgagag cacaaacggt agacgatgtt atttttataa
     2701 aaagatataa tttattaatg gtcgataatt atgaaaggaa aaatattctt aaaaaaaaga
     2761 gtataggatg tcgtatgaaa atagtcgcta tagtaccaat gaagttaaat aacaaaagat
     2821 tgccacaaaa aaacacgaaa gcttttacca atggaaaacc attgtgttat tatatacttt
     2881 caacactttt aaaagtaaaa cagattgata aagtgtatgt ctattgtagt aacccagata
     2941 ttaaagaatt tataccagaa ggtgtaacat ttttaaagag atctagttcc ttagatcagg
     3001 atacaacgag tattacacag gtattagccg gtttcacaaa agaagtagag gctgatgtat
     3061 atgtaatgac acacacaaca gcaccattta ttagcgaaaa atctattgaa aaaggactag
     3121 atgccgtgct gacaggagaa tatgattctg catttgctgc aaaaaaattg caagatttct
     3181 tatggaaaga cggtgaacca tttaattatg aattggacaa tattcctaga acacaggatt
     3241 tgccgccttt atacgaagaa actagtggat tttatattta taatcgcaat gtgatgaccg
     3301 agttgcatag aagaattggg aaaaaacctt gcatagttga agtaggtgag attgagtcag
     3361 ttgatattga tgaagccgag gattttatga ttgctgatgc gatttataat catcttattt
     3421 tacaaggtag ttaagacagg gggaaggtta ggatgaacaa tgtacagatt ttagattgca
     3481 cacttagaga tgggggctat tgtaacaatt gggaatttgg atttgataat acaaaaaaaa
     3541 ttatttctgg acttatagaa tcaggaattg agattataga atgtgggttt ctcacaaata
     3601 aaacagtata caataaaaat gttacaaaat atacgacctt agaagaatta gcacagataa
     3661 ttcctgaaaa aagagaagga aaattatttg tagcaatgat gaactatggc gaatataata
     3721 tagaggatct tccagattat gatggttcat ctattgatgg aattaggatt gcgtttcata
     3781 aaaaaaattg caatgaagcg ttgaaattat gtcgtgagat aaagaaaaaa ggctacttag
     3841 tttttgttca agctatggta tctctcaatt atacagatga agagttttta gcagtaataa
     3901 aaaatgtgaa tcagtttaaa ccatacgctt tttatattgt tgatagtttt ggaatgatga
     3961 aaggaaaaga tttaactcgt cttttttaca tgattgagca taatctagaa aaagatatac
     4021 agataggttt tcactcacat aataacatgc agcttgcata ttctaatgca caaaaactta
     4081 taaccgtaca aacaaatcga agcctgatta ttgattcttc ggtgtacgga atgggaagag
     4141 gagcaggtaa tcttaacaca gaactatttg tagaatattt
//
LOCUS       DAJSBM010000127         4098 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJSBM010000127 DAJSBM010000000
VERSION     DAJSBM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.133"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(514..879)
                     /locus_tag="ONT67_002754"
     CDS             complement(514..879)
                     /locus_tag="ONT67_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356447.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(955..1200)
                     /locus_tag="ONT67_002755"
     CDS             complement(955..1200)
                     /locus_tag="ONT67_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356448.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1270..1524)
                     /locus_tag="ONT67_002756"
     CDS             complement(1270..1524)
                     /locus_tag="ONT67_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356449.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1688..2011)
                     /locus_tag="ONT67_002757"
     CDS             complement(1688..2011)
                     /locus_tag="ONT67_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356450.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2198..2271
                     /locus_tag="ONT67_002758"
     tRNA            2198..2271
                     /locus_tag="ONT67_002758"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2232..2234,aa:Ile,seq:cat)
     gene            complement(2656..3153)
                     /locus_tag="ONT67_002759"
     CDS             complement(2656..3153)
                     /locus_tag="ONT67_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCR2356451.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3193..4002)
                     /locus_tag="ONT67_002760"
     CDS             complement(3193..4002)
                     /locus_tag="ONT67_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCR2356452.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1370 a          725 c          721 g         1282 t
ORIGIN      
        1 tttcaggggg ggaaccttat gataaaaaaa ctatttttac tatcaatatt aacgctagca
       61 atggcaggtt gttctgactc tggcttagca actgaactga tcacaaaatc aactgaagcc
      121 acgacagagg agttaaaatg tggaaacgat cacagtgact acagaggctt aaatcgacga
      181 tgcatgggaa tattaggcag aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt
      241 agaagaagca acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg ctgtgcatcg
      301 tatacagacg tcgaagatgc ggtactttta aaccacgtca ggagatgttc atttatgtct
      361 agggaagttg gcgatgaacc aaaactagag tacgctttta gttttgcacg tgaggatgaa
      421 gataacccta aaacttacga cattttagct tgatctaaga tggttaaagc tttttaatga
      481 agtaagagga gatagaaggc ttttctagtt atattaggta agataaattc ctttttgctc
      541 caaatttgct aaacattctg ctagataaac ttcatcgtgt tccggataaa tcggcacgtt
      601 gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat acataggatt
      661 tttccactct ggtgctgggc tgtaaccacg tttttccatt tcatccatga caagctgatg
      721 atattggaat aatctataag gagaatagtg aaaaacataa tccacagtag catgtttctt
      781 tccccagcca tttcctctta atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg
      841 aggaagcttt gaaatcaatt tttcatgcca taatcgcata gaaatgtctc ctttatatat
      901 agattgttaa ccttagacgt taaatgaaaa aaccagcacc aaggctggaa aaaatcactt
      961 cagtttgctt gctgtttctg agcgaccaat taggtaatca acggaaatat cgtaaaagtc
     1021 agcgatatag attaattcaa tgatgcttgg aatttcttct ccttcttcgt acttacgata
     1081 tttgaataaa ggaatactta acgcgctaga aacagctact tgagataaag atcggccagt
     1141 tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa atagcttctt cgatactcat
     1201 ttaatcacct ttcattatag tagaatagtt ataatgacta tagaaagcta cacatgcaat
     1261 caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga
     1321 caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt
     1381 aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc
     1441 tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa
     1501 caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat
     1561 aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa
     1621 tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt
     1681 gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa
     1741 agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat
     1801 atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca
     1861 tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg
     1921 aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg
     1981 caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt
     2041 ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata
     2101 agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta
     2161 tagttgaaaa gtaacaaaaa taaattttat ttatgatgta cctttagctc agttggttag
     2221 agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtac ataagaggtt
     2281 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
     2341 ttttacttgg ctgttcttat tactgcagat gcaagctttt gaataccgtt taaggaagag
     2401 gttgtgaaat cagcgttttg acctgagtat tagcttaaca aacggaaaaa tagttcctca
     2461 tattttgccg tttgttaagc taataccgag ggtcagctga ttgaacacca tttaagaaag
     2521 aggtcgtgag cctgccgttt cactctgtgt cataaaaaag ccaatctact ttgatagaaa
     2581 aagcacgtag gactaaaaca gttctgcgtg ctttttttat tgtgtgttaa atatcgtaaa
     2641 tgaagataga tatttttatg attcgacgat agagacgttg aaatctttca acagctctaa
     2701 agcggattct ccgtgttttc tactggagac taacattgga tcaacaaaaa agtctgcttc
     2761 aggaaaagca ctctgcaggc ctatcagatt agagagtaca cacaagtttg tttcaatccc
     2821 gactacttcg atctcttttt gttcttcttt gctttcaaat aaagtttcct ggatttcagc
     2881 taaagtttca ggtggaatcg taaagtaata ctttttaatc actcgttcat tttcttgtgg
     2941 agaaagaaga gggatcaatt gcaaatcttc tcgttcttct tctttgtcct tcagttcaac
     3001 tggtatatcc cgtgtgaaga gaatatattc attattttgg cgtgctttag ctaatctttg
     3061 agcaatacgt tctgccaatt gatctgactc tggaatataa aattcgctgt ttggatcaag
     3121 aatatgattt tgcatatcaa taataactaa cattttcttt cctcctacat tcatatcgtc
     3181 attttattct ttttaataga agttttttaa aatataagca acaccagctt cttcattcgt
     3241 cagtgttact tggtttgcaa tttcttttac ttgtcgctgt gcattcccca tcgcaatact
     3301 caacccgctg taattcaaca tagcaatgtc gttttcttga tcgccaaaag ccatgatgtc
     3361 actcgattcg attccaaggt aatccgccag aaaaacaagt gaatgagctt tagtgatacc
     3421 tgtaggcgtg atttctaaga aatgcggatc agagaatact gattggatac catctgcttg
     3481 cagatctctt tgtagcttct gtagtttttc ttcactctta tctgttaatg ctaatttaag
     3541 atagctaaga gaaggatctt ccaacatatt taaaatttga tcaaaagaat ggttctgtaa
     3601 attgaaatgt tcataataag ggtcatcttt gactagattt gattcatcga atgtcagctg
     3661 atcatcttgg gtttctcggc tagcaatgat ggatattttc gcttcttgag catagaagaa
     3721 ggcttttctc agtgtatcgc tatctatttt tgaacgtctg agaactttcg ccatttttcc
     3781 atccttcatt tcagcaatca gtgaaccatt ggaagaaatc aaatagccac cgattcctaa
     3841 ttcggaaaaa atatctttag cagaaatgct gttccttccg gtagcgacgg ttacgattcc
     3901 gccattttca tcaaatttcc gaagtgcttg gatattttct tctggtatct ttccatttcg
     3961 atcaagcaag gtaccgtcta aatcaatcgc tgctagtttc attttatctt cctactttct
     4021 ttttacatat ctattttcct gttaccttaa gattaaatga gaatttaaaa aaatgaaact
     4081 aacttgcttt gtcattat
//
LOCUS       DAJSBM010000128         4050 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJSBM010000128 DAJSBM010000000
VERSION     DAJSBM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.134"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(70..612)
                     /locus_tag="ONT67_002761"
     CDS             complement(70..612)
                     /locus_tag="ONT67_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCR2356453.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            747..1136
                     /locus_tag="ONT67_002762"
     CDS             747..1136
                     /locus_tag="ONT67_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356454.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1140..1904
                     /locus_tag="ONT67_002763"
     CDS             1140..1904
                     /locus_tag="ONT67_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2356455.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2033..2980
                     /locus_tag="ONT67_002764"
     CDS             2033..2980
                     /locus_tag="ONT67_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR2356456.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3110..3850
                     /locus_tag="ONT67_002765"
     CDS             3110..3850
                     /locus_tag="ONT67_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356457.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1351 a          687 c          815 g         1197 t
ORIGIN      
        1 aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga cagtttttaa
       61 gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac cacctgaaat
      121 atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga tcaggcgata
      181 cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct caccttccag
      241 ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac ccggcaaact
      301 tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa
      361 tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg catagacaaa
      421 taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga tataaatcgt
      481 gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg tcaacatgaa
      541 acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc ccaacatttt
      601 tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa aaaaaatcgt
      661 aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat tataatagaa
      721 ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc
      781 acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa cagccaattg
      841 aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta agcgtttttt
      901 tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt ttgaattttt
      961 ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct ctgtgcttat
     1021 ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg ttccagacaa
     1081 ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt gagtaacaat
     1141 tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa gttgttctga
     1201 aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg cagacaagta
     1261 aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc
     1321 cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca tcctatacat
     1381 tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag catttagaaa
     1441 aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa
     1501 catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg atgggttccc
     1561 ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat aattatgtcg
     1621 taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg tcattgcagc
     1681 cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac gaaaaaatcc
     1741 cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa
     1801 ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta atggaagaag
     1861 cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa cggaaaatca
     1921 ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact ctgttaggaa
     1981 aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga gcatgcccac
     2041 tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa
     2101 tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa tggctgaact
     2161 ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa
     2221 attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt tgctgatggg
     2281 aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa atgatgcctt
     2341 cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag acttcgattg
     2401 gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag gatatgatta
     2461 tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct tagaaagaga
     2521 ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa tttcaaggga
     2581 aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc
     2641 aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt ttacagataa
     2701 aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag ggattataaa
     2761 tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg tatttttaga
     2821 ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta tgggtgaagt
     2881 atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg
     2941 tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac tatatatata
     3001 tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga
     3061 atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat
     3121 cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa
     3181 cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc
     3241 cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg
     3301 caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca
     3361 taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg
     3421 aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc
     3481 caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt
     3541 tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga
     3601 tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc
     3661 gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa
     3721 aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt
     3781 agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta
     3841 ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc
     3901 gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt tttttgagtt
     3961 taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa aacaaactat
     4021 tttcgcagat cgtttgcaaa aatagtttat
//
LOCUS       DAJSBM010000129         4036 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJSBM010000129 DAJSBM010000000
VERSION     DAJSBM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.135"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(242..391)
                     /locus_tag="ONT67_002766"
     CDS             complement(242..391)
                     /locus_tag="ONT67_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356458.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="ONT67_002767"
     CDS             853..1065
                     /locus_tag="ONT67_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356459.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="ONT67_002768"
     CDS             complement(1343..3142)
                     /locus_tag="ONT67_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR2356460.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="ONT67_002769"
     CDS             complement(3266..3862)
                     /locus_tag="ONT67_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR2356461.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DAJSBM010000130         3987 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJSBM010000130 DAJSBM010000000
VERSION     DAJSBM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.136"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..938)
                     /locus_tag="ONT67_002770"
     CDS             complement(<1..938)
                     /locus_tag="ONT67_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCR2356462.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKD"
     gene            complement(1160..1522)
                     /locus_tag="ONT67_002771"
     CDS             complement(1160..1522)
                     /locus_tag="ONT67_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356463.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1526..1864)
                     /locus_tag="ONT67_002772"
     CDS             complement(1526..1864)
                     /locus_tag="ONT67_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356464.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1843..2262)
                     /locus_tag="ONT67_002773"
     CDS             complement(1843..2262)
                     /locus_tag="ONT67_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356465.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2255..2488)
                     /locus_tag="ONT67_002774"
     CDS             complement(2255..2488)
                     /locus_tag="ONT67_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356466.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
     gene            complement(2491..3459)
                     /locus_tag="ONT67_002775"
     CDS             complement(2491..3459)
                     /locus_tag="ONT67_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2356467.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
BASE COUNT         1131 a          892 c          549 g         1415 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcgctctctc agaagccatc agagccttat cacgctcgtt ttcttctctt
      121 tgcctataaa tatacctcaa ctcatcagaa acgctctcac gctcttttac agccgtttgg
      181 cgcaattcag aaatttcact catgatttct ttgtattcgt gaatatcttt aattttgttg
      241 gtaccgacaa tatccctttt ccaaccgtaa tcattcatta atcgttctat agagtcaact
      301 tcttgatttc taaaattatt gaaaagcact cgaccattat ctttatcagc gtgtatccct
      361 tgttgaacca gtgctttatt aaagctcggc tgcttttgaa caccccgttt ataaccttca
      421 gcaaccggca caatattcaa gtgcaggtgc ggacttattt cgtcattatg aataacagca
      481 ttataaacag ccaaattagg gtttctctct tgaaactcct tgtaatactg aaccaaaatt
      541 ttattggcaa tcactcgatt ttgttcagct tgttgtttcg atatgccagt agaagaacca
      601 tctgctcgat ttgtctggaa atcatctaaa atgcctattt gcacaataaa ttctcgttga
      661 ggttccaact ttttatcctt gcgcactttc gctaaatagt cctcgatttt tctatcggct
      721 cttttttgtt tcgcattata tttttctacc acttcgccaa acaattcctc atatttatct
      781 gctagattcg tctgctctaa ataaatattt tcatccgatt tcgtgaagtc aatgtgctta
      841 tgccaatcat tggttttctg gcgatcagtt aactcacgat tattatgaga aatacttgta
      901 ttcttcgttc cttttttaaa tgaaatacta aaccccaagt taaaaccctc cttcgcgctg
      961 tcgctaataa aaagattgta aacctttctg ttactctttg tcaaagagta acattttcct
     1021 aattgtgaca tggatgtcac aattccaaac gctctccgag ggttttcgct gtcacaagtg
     1081 acaacgaaaa aaggagccga aagcgtatca cgctatcaac tccttttatg ctttattttg
     1141 cttcacgcaa aataatcgct tatcgtaaat tcacgtcatt aaatattgac gtaaatttac
     1201 tctcactcat ctgacttttc ttattcggta tcactccgaa taagtcgtca gattcatcgg
     1261 gataatctcg ttcgcttggc gctgattttt catcagcaag ccactcatta tccactgttt
     1321 tttcgtgttg ctgatcgtca gaaattgacg ataaccaccg ctcaaaatca gcaaaagttt
     1381 caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc tcaatcaagc
     1441 gttttttctg ttggcgaagt ttcgcttgtt ctttttttaa tttttcttct tgcgctttaa
     1501 tatctgctaa agtcattccc ataaactaca tctccttttc tttggaatta ataacggaaa
     1561 tttggtttaa ctgcataaca tgactcatta atttcaacca aaattctttt tctgattcat
     1621 cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa gattttgcta
     1681 gatcagcatt gataaccgag ttgagaacat ccataattcg ttcaagcatt tcactataat
     1741 tatcttcatc cggcaaattc aaataatcag ctaaccgttc atacatcaat tcaattctat
     1801 cgactgtttc ttctctcaaa tcaattttat ccatgtcaca cctcacttcc agaattgcca
     1861 ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt tttgttgctg
     1921 aagcaaagtc aatttttgtt ggtcacgaat catttccgtt aattcagaaa tatgcctatc
     1981 tttttccgct aattgttcac gcaaaaattc attttctttt tctaacggga aatcattgcc
     2041 tgcatcattg accggtaaat caccagtcaa ttgaccacct tttgaccgat aaattgaccg
     2101 cacaacacca atcaattcct tgtctaacat gataacccca ttatctttat accgagcaag
     2161 gtcataacct gcattgacca gttgattgcg cactgtattt ttagatatac ccaactcatc
     2221 agcaaggttt tttatggtca ttttttctgg caaatcagtc attatcttct ccgttacttt
     2281 cagctacccc ataaacatat ttaacagcat gcttagggat aatgattggg gaatcaattt
     2341 tattattttt caaaatcaac tcatactgcg ttacatcaag taacttaccg gacatttttt
     2401 tgctatccaa aaaaacaacg acaacgttat cacctgttaa attcaccaac caatttttct
     2461 taccaaaatg cttttgtttt tctgccatta ctattcctcc tctgtcacat caagtccctg
     2521 taaccaagta tccaattctt tttttaaatc atctgctgat tcttcaaatg tttctcgtaa
     2581 atgattcgtc aactcatcat tttgactttc tcgctttaat aaatttcgat atttatattt
     2641 cgagtttctt gacaaaatcg cccaattaga atgattagct aacaatccat caatcataac
     2701 tttatccgta ggttttatat cagttggtaa aaaatgtgga ctcacaaagc tatccaaact
     2761 ttctttcacc atagcatacc attccgtagc tttaccacgt cttaactgca tttctaaccg
     2821 ccaccaagta ttaatttctg aaggcaccac ctttcttttc atttcttgct ctattttttt
     2881 attgtacaaa cgaatttgcc gctcgcttgc ccgactaccc caatacaccg tttccagttt
     2941 tcctgagcgt ccataaatta atttaaatga aactggatca ataattcgat attgcgtgat
     3001 aaactcatca ggcacatcaa taagatcgca ggcaacatcc gctcgtgaaa aatggggctc
     3061 taaaaataaa tcatgaataa aatttttcat ggaatccgct aaaaatcgac tgattttatt
     3121 aggattaaaa tcaatccgcc cttttccttc ttgccattta gaaatttcaa tataggcaat
     3181 attttcgtca tgttggtcta atagctgcca accattattt ccaaccgctt ttaaatagcc
     3241 attgccttca tttacccgca taagctgttc aaaatctaat attaatacat catgttgagt
     3301 atgataaaaa atattttgat tcaactttcc aacaatggta attcggtcaa tcgaccaatt
     3361 taattttata gtttgtacca aagtttgaac atttaaccgc ctgttagaca gggctgttaa
     3421 tttttcccac tcattcacga ttgttctttt tttactcata ttcagttcct cctcaaagaa
     3481 aaaagagatt tttggaaaga atttccagag ttcacgtaac tctaaaaatt cataaccatc
     3541 aatctctagt cttgaagaaa cctaaaaata ttcgtataat gaatttgagt tacggaacta
     3601 gtgtggttag tttccgtaaa ggtcgagaac gttggtagcg ttgctcgact tatttttttg
     3661 ctctttttcc agttcatttg tttgctaact caataatagc acttctcttt agctatgcaa
     3721 ataccaaaaa aataaaaccg caccctttga aaagggcgcc cccaaacttc gctaacgcta
     3781 agttggccaa aaaatggact tgccattttt tagcaaatgt cagcttcagg ggctagtggt
     3841 ttagccgtca actccttatc ctcggcacaa accgcctccg gtaccgttga ctgcacatcg
     3901 tggctaccac tcaatctcat tctcagaacg ctctaattta tgctgtgaga cgttttgacg
     3961 ataaaataag gttttctcct gctctaa
//
LOCUS       DAJSBM010000131         3849 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJSBM010000131 DAJSBM010000000
VERSION     DAJSBM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.137"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..265)
                     /locus_tag="ONT67_002776"
     CDS             complement(<1..265)
                     /locus_tag="ONT67_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR2356468.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQ"
     gene            complement(307..1365)
                     /locus_tag="ONT67_002777"
     CDS             complement(307..1365)
                     /locus_tag="ONT67_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HCR2356469.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(1484..1939)
                     /locus_tag="ONT67_002778"
     CDS             complement(1484..1939)
                     /locus_tag="ONT67_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356470.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(2523..3326)
                     /locus_tag="ONT67_002779"
     CDS             complement(2523..3326)
                     /locus_tag="ONT67_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2356471.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1152 a          727 c          514 g         1456 t
ORIGIN      
        1 attgtccgat tgataattta tatacttgta ctttctttcc atcttgtcgc tgtctttcag
       61 cccattcact tgcaagtgct gttttacctg ttccggcttc acctaacaag atagcttgcg
      121 gtgtttccgg tctattcaag atcagtgaca actcatggag ttcgtcatca cgtcccacta
      181 acttatgttt tggtggtttc attggtgtga gagcatgatt aaattcggtt tgaatattct
      241 taaaaaactc ggtactattt tccatctgtt tgtgttcact tcctctaaaa taagaataat
      301 ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc caaaagaatc
      361 tttgttaaag tcagcatttt tgtattgatc aatcaatttt tgtgtatcat tgattgcttt
      421 aatttcggtc tccattcgac tgtctagttg acgaatactt tcttgcgaat ctttatcgtc
      481 aggattttgt tgtaaacgtt ccagatactc agcttttgtg gcttcaattt tcttgatgtt
      541 agtatttgaa gcatttaact gatcttctag cttatttaca acattattct tacttgtaac
      601 tgctacaact tttgaataat caatatcgcc atctttggta agaaagcttc cttgatagac
      661 attagttgaa tctgaatatg gatttattct gaaattgata ttatcaggag tattaacagg
      721 ttttgctttt ttcccaaaag aaattactcc ttcattatta acatcattca tagaatattg
      781 agaaagtgct tttgtaactg aatctgcttc attcacattg actttggtat catcgtcagc
      841 tgtatcatca ccagaaatta gtgatacttg gtttgcaata aaaacttgat aagctctttc
      901 ctctaattta ccttttagga ataagaaagc atccccatca gtacttaaca tcccatagct
      961 catttcaagc ttcttaggac gatctttttc ttttgttgcc atatataagt tatagttttt
     1021 tccttgcgta gataacaact ttctagcgtt ttgggagtaa ccaagtttta ctactgttac
     1081 atctctattt tgatctgtcc acacatctga aatagtgatt tctgctcctg atcgagcaaa
     1141 ctccaatgaa gaccctatag gtgttgtatt gttcacgaga taataattat agtttcctaa
     1201 ttgataaatg tagctaccca agcctgttaa taaaaaaagc actcctagaa aagttgcaag
     1261 cataatgcgt aatccgtgac gtgttctctt atccttatca ccaagcaacc ctccaataat
     1321 actgtattga tctttggtag atgttgaatt gacgtttttt tccatcagtt tgtctgcccc
     1381 ctttcataaa agagcaaaca atcagacgtt ttttcggtaa ttctaggttc gggattttga
     1441 atcatttcgg tatattcttc cccattaata tttaacacaa aaattatctt cctttcttcg
     1501 ttcctttctt ttgctcttta ttttccaaat aatcaatgat ttttgctatt gtaccaagat
     1561 agtaattccc ctcagctttg ttttttcgga ttttgctaac cattggagta ggtacaatct
     1621 cttcatctct caattcactg aaagattttt tacctagata ttttccaata acagatttta
     1681 aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc ttttagttca tggttcatat
     1741 tgtcaacgac ttttgcggtt tcaataattg ttttagttct cagcttaata atttcatctg
     1801 gaattttcaa tcctcgtaat ttcatcgttg tactaactgg taacccatgc tttactgcat
     1861 cagcaatact aaaacttttt agatcatcta gtaagctctc catatagtta acaaattcat
     1921 gccaattaat agcattcatt gtctcacccc ttcatttttt taaaatgagg ttcgggcgta
     1981 acacatttat gttattataa tatcataata acataaataa atcaatcagc aaatttagat
     2041 atcccttaaa gtatcagcaa ttattcaaca agatatttat tttagctatt tcagtcatca
     2101 taaaagacta attagcaata aaataactaa tattatccat ttatcagacg aaaagaaaaa
     2161 acttatctaa ttactctata ttaactttta acaaatagtt gacttgtcca aaaatgttac
     2221 cttcaatctc acaaagatat taagatgttt cctaccaaaa ctgatatcac cggacttttt
     2281 attctgtgtt gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa aaattgtttt
     2341 tttcttgact tgtccaaaag tgttacccat agttgttaaa atttgtatag attagccatt
     2401 ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa acttcttgca tttattgtcc
     2461 aaaagtgtta cccatacttt tttaaagaag caggttgtat taaaattaca acctgcttct
     2521 tctcaatctt cttctaacca attatgcatt gaaatggttg gcaagtttga ttctggctca
     2581 ctaaaagtga atctgagccg atcaactctt cttcctttct ttgcatagat tttttcataa
     2641 tcaaaagttt caaatacctg tattccgttt tcatcaggag ctaaaaattc ttctttcgcc
     2701 tgctctagta ttcttttgtc aatatttgtc attctataac ttgaaggtat acttaatgct
     2761 cttctaaaat cttcaacttt gactacccaa taaccatttc gccactcttt atttctgtat
     2821 tgcatgagat gacgatacaa ttcttttgtg tagctacttc ttagttttgt aaaattatca
     2881 agttcaaaac gagtaaagtt tttgccaatt gagtttaaga taaattcaaa ttcctcattt
     2941 gccttaattg taactgtttc cttctgttta tctatccgga atttttgaaa aagccacagc
     3001 tgttcataga cgtctgaatc ttcaaaacgg taggttaaag tacctagttt gtcactcaaa
     3061 gcttttaaca cagaataaaa actgtccttt tctcttctgt cgtatttgac caaataacga
     3121 aagaggttga aatcaaaagt tacttctgct gttccttgac gtttcatttt tgataaaata
     3181 gcccaaaaca catccatttc aacaggctta aatcctcgca aagctgttgt attcagacca
     3241 ttgttataac gcacaattgc atttgtttgt tctgatacta aatctacatc actatcaata
     3301 atcttttcgg taacttcttg agacattgat tactctccaa tcttcttttt gcgctccttt
     3361 aatcatgtta atctggtact aattaaagga aacaattttt tctgcttgtt acctaaatta
     3421 aatcacaact aaaggtaaca gtcaacaaag atttgttact aatctaattt tctatttttt
     3481 cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata cctcaaatgt
     3541 ccacttttgt tacctttgct gtccaaaagt gttaccctta ctgtccactt ttgttacctt
     3601 tactgtccaa aagtgttacc tttatacccc tttaatactt actctcccaa gagattaagc
     3661 ctcccctaac agatatatta acagatattt aacagatata ttaacagata tttaataaag
     3721 gaggattact cctctcttat ttcaaaatct taaaaataaa aaatcttaaa aaaaactaac
     3781 actatctagg agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa ggaaaaggag
     3841 gaagcttca
//
LOCUS       DAJSBM010000132         3742 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJSBM010000132 DAJSBM010000000
VERSION     DAJSBM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.138"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..115)
                     /locus_tag="ONT67_002780"
     CDS             complement(<1..115)
                     /locus_tag="ONT67_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2356472.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="ONT67_002781"
     CDS             246..698
                     /locus_tag="ONT67_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356473.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="ONT67_002782"
     CDS             712..1401
                     /locus_tag="ONT67_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356474.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..>3742
                     /locus_tag="ONT67_002783"
     CDS             1526..>3742
                     /locus_tag="ONT67_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCR2356475.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFF"
BASE COUNT         1261 a          507 c          770 g         1204 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tc
//
LOCUS       DAJSBM010000133         3715 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJSBM010000133 DAJSBM010000000
VERSION     DAJSBM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.139"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT67_002784"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ONT67_002784"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR2356476.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ONT67_002785"
     CDS             complement(2262..2513)
                     /locus_tag="ONT67_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCR2356477.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ONT67_002786"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ONT67_002786"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCR2356478.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ONT67_002787"
     CDS             3463..3681
                     /locus_tag="ONT67_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356479.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1026 a          797 c          688 g         1204 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caaga
//
LOCUS       DAJSBM010000134         3583 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJSBM010000134 DAJSBM010000000
VERSION     DAJSBM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.141"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            332..1372
                     /locus_tag="ONT67_002788"
     CDS             332..1372
                     /locus_tag="ONT67_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCR2356480.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2196..2528
                     /locus_tag="ONT67_002789"
     CDS             2196..2528
                     /locus_tag="ONT67_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCR2356481.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3147..3488
                     /locus_tag="ONT67_002790"
     CDS             3147..3488
                     /locus_tag="ONT67_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCR2356482.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1322 a          473 c          667 g         1121 t
ORIGIN      
        1 tattactagt aatggtagta tgttttatat gcataaggta gttcttaatt tattttttgt
       61 tttgacactg gtagtactta attatcttta attttaaaac taaattttat tcatacgttt
      121 ttctggtagt atttaattat aaataatatt tatttaaagt agttcttaat gactttaaag
      181 gtagtacata attcatttta tttctgattt taggtagtat ttaattaaaa aacagtttta
      241 atgcgtttgc attttttatt tatctttgct attttcgaaa agagtaggta attttacata
      301 aataaaacaa cgtgaaagta gaggatctac aatgactgat tttaaatttt ttaaggcgga
      361 tagagtttac aacgaattat tttatcaatt tccaaaagtc tttattgttt ctgacgaata
      421 caaaaaaatg aaagattcaa ctaagattgc ctatatgctt ttaaaagcaa gattagagat
      481 cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt tattttactt atacgacaaa
      541 tgaactatgt agagtattaa actgccaaaa acaaaaagcg atagcaatca aaaaagagtt
      601 ggaatccttt ggtttattat tacaaaagca gatgggattt aacaaacagt tagggaaaaa
      661 taatcctaat agactatatc tagcagaatt aaaagtctca gaaaatgata tctacttact
      721 cgaaaaattt gatagagaga atagggaaaa cgttgataaa tcagagggta tgaaaatcat
      781 acccaccctc gacgaaaaat cagacgctga atcccttggg gctcaagagg gtatgaaaat
      841 cataccgtgc caaaacgttg ataaatcaga gggtatgaaa atcataccag aacttaataa
      901 taatatatta gacactaata gacacaatat agacactgaa aaagaccgcc tacaagatca
      961 attgttgtta gacaattttg agacaattat gacaaacgac agcattgcta cgtttgtccc
     1021 tgaacgagta ttaaatttga taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac
     1081 cgtccagact attcataatg caaagaaaaa agctgaaata gaaagtggta tttcgatagt
     1141 ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa gaattataca cgacactgtt
     1201 aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt
     1261 ctatgtaaaa aattggttta tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg
     1321 tttgaattat gaaagctccc caagcactat tacgaaagac tggttagagt gaaactttac
     1381 tgacaaagtg attgttgctt catggagtaa cattaaaatt aaaaattttt tattattcta
     1441 attatattac gaatattaca aaataatagc tattttaata gaatttataa gttaaaatat
     1501 tatgataaaa atcacaaacg tagcctttta cgtcataagt gttaaaagaa ataattaatt
     1561 tcaaatatag aaaagggaac gtatgtttgc atactccttt tttttgagtt ataataatac
     1621 gcgaagagag ataatttcat tatttaaaca agctaaaaat cttgatatta cttcttttca
     1681 ggcaatacga atattattcg cttacatttt ttgtataaat ttaattgtct gctatgcttg
     1741 tgatgaattg attttcgttc tgcacttttg gatgataaat ttggacaatt gaaaatacaa
     1801 aaaaaagaaa ggcggtatga actcggaagg ctggtaacct tcgtcagcgt tcatacacgg
     1861 cattaagatt tggcccttaa tgcgttaacc tttcagtgtt acggtattac ccgcaatgga
     1921 tttagtataa cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa
     1981 tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt ataaaaatgt ccgtgtggct
     2041 ctttgccgta tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt
     2101 ttttgtttgt catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg
     2161 ttaaaaatat ttaaagaaat tgggtggttt attttatgtc atatacagga attttgagtc
     2221 tcaaagatat ttgtcactac ggtaaacggt gtaccgctac agagaaaatc acgaagaaac
     2281 tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca
     2341 aagtctcaga ggaaatgatt tattacattg ggaaacaaaa gcaaataatt ttaaaagatc
     2401 ctatcccctt aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac
     2461 tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga
     2521 tcggctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata
     2581 ttgtcgagac ttctaaagga acttagagtc actagaaaaa taaggagtag acttgatcta
     2641 tcaacaattc ccttcgggga gtgacggggt aggttttaac gattggtaac gtaaaaaatt
     2701 ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa tgaatttgtg taaataataa
     2761 gcgcagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg tctagattaa
     2821 tcatgagcag ctactaaggg atctagactt tgacgcaaac tcatggaata aggagtaggt
     2881 cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata
     2941 cttgcgagga cgttgattat tggatcaatc gagcatttac cgctgtctac tagggtttct
     3001 cgtttcggac gccataaacg agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg
     3061 ataaaatatg aacaagtcct gaataaagtg aaaaaattga tataaaacat agagaaaatg
     3121 aaatttacgt aatggaggaa tgacaaatga aaaattatga tccgaatata cgattgggca
     3181 cacatactat taaggtttct ttccaacgtt gggattacaa aggatttgtg acgtttagaa
     3241 gaggcggaaa ttgtaaagga ttagatgttt tagcattaga cgaggatgat ttatacgatc
     3301 aaacattgac tgataatcca attggattcg gcttgctacc tgaagatgat gaaggtaatg
     3361 aatggtttaa aatgacgctt atgaatgata atggagacga attatctgtt gaagatactt
     3421 ggtcatattt gagtgattat attgtcagct ttgaaatcat tgaatttgta gcggataaag
     3481 aagaataaaa actggaggaa taaattttac agataaaggc ttagaaggag tcacgatgat
     3541 taataaaaag gtagtattac cttaggcatt gagtaaggaa ttt
//
LOCUS       DAJSBM010000135         3563 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJSBM010000135 DAJSBM010000000
VERSION     DAJSBM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.142"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..2232)
                     /locus_tag="ONT67_002791"
     CDS             complement(<1..2232)
                     /locus_tag="ONT67_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356483.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ONT67_002792"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ONT67_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1018 a          783 c          551 g         1211 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaa
//
LOCUS       DAJSBM010000136         3552 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJSBM010000136 DAJSBM010000000
VERSION     DAJSBM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.143"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            577..909
                     /locus_tag="ONT67_002793"
                     /pseudo
     CDS             577..909
                     /locus_tag="ONT67_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="ONT67_002794"
     CDS             923..1072
                     /locus_tag="ONT67_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356484.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="ONT67_002795"
     CDS             1069..1515
                     /locus_tag="ONT67_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356485.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="ONT67_002796"
     CDS             1517..1654
                     /locus_tag="ONT67_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCR2356486.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="ONT67_002797"
     CDS             1691..1984
                     /locus_tag="ONT67_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356487.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="ONT67_002798"
     CDS             1981..2205
                     /locus_tag="ONT67_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCR2356488.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="ONT67_002799"
     CDS             2202..3221
                     /locus_tag="ONT67_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCR2356489.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="ONT67_002800"
     tRNA            3410..3483
                     /locus_tag="ONT67_002800"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1223 a          634 c          740 g          955 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tc
//
LOCUS       DAJSBM010000137         3462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJSBM010000137 DAJSBM010000000
VERSION     DAJSBM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.144"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..828
                     /locus_tag="ONT67_002801"
     CDS             <1..828
                     /locus_tag="ONT67_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HCR2356490.1"
                     /translation="LKPLLYIIDEILNSFYQKSYWGYSLPNYISAVHNAHPNYASYLS
                     YKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYMETGKIQEEYMEELRKFLSDK
                     KILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSHIDIDYVFLSNLRRFHELPKE
                     SRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGLMAIRFLADMNVDEIFLAGFD
                     GYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSELAKKVKVTFLTDPRWVRIDK"
     gene            963..1541
                     /locus_tag="ONT67_002802"
     CDS             963..1541
                     /locus_tag="ONT67_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356491.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1487..2005
                     /locus_tag="ONT67_002803"
     CDS             1487..2005
                     /locus_tag="ONT67_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356492.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            2013..3053
                     /locus_tag="ONT67_002804"
     CDS             2013..3053
                     /locus_tag="ONT67_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR2356493.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            3302..>3462
                     /locus_tag="ONT67_002805"
     CDS             3302..>3462
                     /locus_tag="ONT67_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2356494.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1254 a          386 c          680 g         1142 t
ORIGIN      
        1 ttaaaaccat tattatatat aattgatgaa atactaaata gtttctatca gaagagttat
       61 tggggatatt cattaccaaa ctatatttca gcagttcata atgctcatcc taattatgca
      121 agctatctgt catataagaa tactttaaca attgaagcaa tgaatgaaat attcgaaatg
      181 atggatgaag aaaaaaaggt atcttatgat aaacaatata tagaagattt atactacaaa
      241 tatatggaaa ctgggaaaat acaggaggaa tatatggaag aactgagaaa atttctctca
      301 gacaaaaaaa ttctactgat tgcaccagga aaaagttctg tagatgaggt agaaagaata
      361 aaagagtttg ttaccaaaga agaagtcgta ataattagtg taaattttga atattctcat
      421 atagatatag attacgtatt tttaagtaat ttaagacgtt ttcatgaatt accgaaagaa
      481 agcagaacta aatgtattgt tacatcaaat atacaagcat cggatgtata tttacggacg
      541 agttataaaa agttgttatc tgaaagggaa gttgtaagag acaatgcagg attaatggca
      601 attagatttt tagccgatat gaatgtggat gaaatattct tagctggatt tgatggatat
      661 tcacataatg aagatgagaa ttatggtgag caatccatgg aaattataac aaaatttgca
      721 ttattagatg caatgaacgt gcaaatgaga gatgaattgt cagaattagc taaaaaagta
      781 aaggtgacat ttttaacgga tccacgttgg gttcgaattg ataagtagta gtaataatgt
      841 ttttaaaatt ttgataaaga gatagtgaga gaataaaaat gggtaaagtc ttaaataaaa
      901 aagaagaaaa atatttaagg gacgatgggg cgagatgaaa ctctaatttt gaacttttaa
      961 gaatcatttc tatattgtgt attgttgctt accatgctaa tttgcgttta aattataatg
     1021 cagacggaat tatagtacat ggttcattaa ctgctaacca gattttcttg aattatattg
     1081 gtatttgggg tcagctagga gttaattgtt ttatatttat ttcggcatat tttttaagtg
     1141 agaaaagaaa cattcacttt gaaaatgttt tgggaattgt attagaatgt attttttata
     1201 gtgtattagt ttatggaatt ctttgtgcat gtggaataga aacattttca ttgaacagct
     1261 ttgtaaaaga aatattatca tttgctctag gtagatattg gtttattaca tcctatttag
     1321 cattgtatat tctaagtccg tatttaaata tcatgcttaa caatctggaa aaacttcagt
     1381 tgcaaaaaat aagtatgtta ttgtttatag gtgtatgctg ttatcgtatg ttatttaaaa
     1441 catctttgtt tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga
     1501 aaaaaacgat aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac
     1561 agttatacca cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt
     1621 taaccagatg gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta
     1681 tgtatttaaa aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt
     1741 aggggtatat attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt
     1801 aatatgggat gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg
     1861 gtttataatt gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga
     1921 aaaatttgaa agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa
     1981 tcatgttata aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat
     2041 cattcggaga attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag
     2101 atagtttgga gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat
     2161 ttggatatca atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag
     2221 ctaatgcttt gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca
     2281 tgggactgta ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca
     2341 gaaaattttc tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa
     2401 acgatgcaaa ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg
     2461 agacgtcctt agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt
     2521 taaattatcg atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac
     2581 taccattggt tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta
     2641 aagctgagca tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg
     2701 ctagggagat tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt
     2761 attcggcaag tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt
     2821 atttttccac agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt
     2881 ctgctggtat aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg
     2941 cgactgcatc tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg
     3001 aggatgtata taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat
     3061 ataaagataa atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa
     3121 cgtgaaaata aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa
     3181 aatacaaata tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa
     3241 acagtcattt gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtgca
     3301 aataacctat acctatctta caacggatga acttgtaata atagagtctt atttgaaaat
     3361 taaccaatct gttgctaaaa ctgcgcattg cttgaatcgt tcaagtcaaa cgatccataa
     3421 agtatatctg ttcttcaagc aaagaaaatc agccttagaa ta
//
LOCUS       DAJSBM010000138         3391 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJSBM010000138 DAJSBM010000000
VERSION     DAJSBM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.147"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(22..2493)
                     /locus_tag="ONT67_002806"
     CDS             complement(22..2493)
                     /locus_tag="ONT67_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCR2356495.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3391
                     /locus_tag="ONT67_002807"
     CDS             2766..>3391
                     /locus_tag="ONT67_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356496.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          999 a          686 c          609 g         1097 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctcttcttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatgttga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggtgattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accagccacg tatgaacttc cttccgacgg
     3241 tgttgtgtat ggatgagttc aaatcaatga attctgtttc tggttccatg agttttattt
     3301 gtgttgatgg gacaaccaat cagctattta cgatccttga agatcgcaga ctttacaaac
     3361 taactcaata cttcctgcgc tttcctagaa a
//
LOCUS       DAJSBM010000139         3355 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJSBM010000139 DAJSBM010000000
VERSION     DAJSBM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.148"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            313..594
                     /locus_tag="ONT67_002808"
     CDS             313..594
                     /locus_tag="ONT67_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356497.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            596..1012
                     /locus_tag="ONT67_002809"
     CDS             596..1012
                     /locus_tag="ONT67_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356498.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1664..2089
                     /locus_tag="ONT67_002810"
     CDS             1664..2089
                     /locus_tag="ONT67_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356499.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2119..2283
                     /locus_tag="ONT67_002811"
     CDS             2119..2283
                     /locus_tag="ONT67_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356500.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            3241..>3355
                     /locus_tag="ONT67_002812"
     CDS             3241..>3355
                     /locus_tag="ONT67_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2356501.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDV"
BASE COUNT         1133 a          585 c          571 g         1066 t
ORIGIN      
        1 gcaacatggg cccatgttgc gatattttgg tggcgcccca atccccgttt tttcgttgaa
       61 tcttactcgg actgcactcg ttttacagct gatttttctg aattttgaaa gagcataagg
      121 aaacgtggta tcactttctt acgaaaggtg ctagataagc tgtgcctatc acgtttctca
      181 gaaaaaaatt tgctatcact tttttgccat tgtggctagg ctatgtatag tgttttggcg
      241 aaaaatagct atacaaatac tatatttgta tgatatacat aaattatata ctgatacgaa
      301 aggaaaattt ccatggacat tttagttcga aatatcaatc ccaaaattat tacaagaata
      361 gataaacgtt gtcaggaact aacaaccaaa acgggcaaaa aatggagtcg aaatcaatac
      421 ctaaaaattt taatcgaaaa cgattttgac cacgaactta tgaattacaa aaaagatcaa
      481 ttcgatttat tattcgaaaa atttattgcc attcaagcag aaaatacgaa catactaaat
      541 gactatgttc acacaaacaa ccaactcatt gaaacactta tcggaggaat ataacatgag
      601 aaaaacttct gttcaaaaac acgtaaccct ttatcccagt cacgttgacg ttttagattc
      661 catcatcgaa aaaaatccag acgtcaacaa ttattcagaa gccattcgct ttttatgtat
      721 gagttatgaa cctgctacca atacaaccac aaccagtaac caagcaaaac tcaatgccat
      781 gggaaaagaa atttctatca tgacagaaat catggcagaa tttgctgatt tatatctaaa
      841 agatactggt gttcttatcg gtacaaattc taaagtatat caagaagtga aagaaaaaat
      901 tgaaaatcaa ctcaatcctt cccgacgaaa tcaacccaaa aatcgtcaaa aagaaaccaa
      961 tcaaaccttt acttctggaa attttgaccc aacaaacttt ctaaaaagat aaaaaaagaa
     1021 accgaaaaat aaaaaaattc ctcgatttct ttttagggat cggtcttcca gcaagatttg
     1081 caagttggta ccaacttgcg aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt
     1141 ttcattttgg ggcgctgccc taatccctaa gtcccggtta ttcaatagca tggaaactca
     1201 aagaataaga cgttccctgg aataaaaaaa aggctgctcg ttcgagcagt ccatacatag
     1261 gaaaatgaaa aaacgacgtc ccttactgag aaacgctatt aaacttttaa caaatcctct
     1321 aaaaaataac aagtctttaa aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga
     1381 aattgcatca aatcaagcat tagaagctag taactagcta gaccaattga aaatcacaaa
     1441 gtgagcgcct gtatctattg ctacaggcgc tacttggcat agtgtacatt atgtcaacaa
     1501 gaatttaata taacatattt ttctagtttg ttataacaat gccactctaa caaaaatgta
     1561 agcattatcc ttattaaaag ctccttgaca aggactatac cttttatttt atgattttta
     1621 catagaaaac gttttctcta ttttttgttt agaggtgatt tttatgattt gcaagaacaa
     1681 gaaaaaaatt attgctatta ttgctagttt ctctttgatt tttagtactg catgtactgt
     1741 cttattaaca aataatgcta atgttgaagc tgcaagctct tcttacaaac ataatcatgc
     1801 tggttataga tgttacaaaa tgagttatta tgtaagtcct tctaaatgca aacaattggc
     1861 tcgccaaact aataaactta ataccgtctc agctattgca aactttattg gactatctgg
     1921 attaacaccg gggattatat cattagtttt cggatcagca gttagtgcta ataatgtatt
     1981 tgttacagcc gctagaagag gaaaaggcgt tcaattaaat tatattgccc attttagtaa
     2041 taccactacc tataattata gttctaacca aaattatgtt ataaaataat taaaacttta
     2101 aactaggagg aatgctttat gaaattaaca aaaattgctg taattttagc tgttattgct
     2161 ggcattattt tatattttgt attgcctgct aatcaattct tcctttactt tggaatacct
     2221 gcgattttaa tagtaaatgg cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc
     2281 taatcgttac aaaacaacaa tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg
     2341 gctagcaaat gctagccttt tttgtattgt agagcgtgaa ttttgagggg gattcaaggg
     2401 ggagcttgcg cccctttgac aagcggttat ttgcaccaaa tacctccgta tttggctagc
     2461 aatccttcta aaaaatacac ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc
     2521 gaatccgaat ttgcaaagct gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac
     2581 atatggctat aatttgcgtt ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa
     2641 acaactttaa aacgccttag atctaatttt agaggtctta tagaacattt tagggttgtt
     2701 agggcttgcc ctgaccgcct gtaaggcgct ttgagaacaa gataagggtt ttatactaag
     2761 actgtcttaa aacggcttaa acaaacgctc agaacttttc aaaggagttc tttatgaact
     2821 gtttttaagc aaaggagcct ttaggcgact gcgcagtata atgtgagagc gtagcgatca
     2881 cacattttta gtgaccgtag ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt
     2941 tgaggaaaaa agcaggagtt ttttaggcgg gaaattttgc ctcctctttt ataatctctt
     3001 tttaaacctc tttttaaacc tctttaccgt cggctgtatg cttactctcc caaggtgtgc
     3061 agaatctttt gggggtcttt ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt
     3121 ctttttgtct gtttttgggg gtctttttgt ctgtttttag gggtctttaa ttaggggctt
     3181 gacccctatt agaaagctct ttataatttt gttaagtagt taatcttgaa ggaggatttt
     3241 atggcaaatg aaattgtgaa ataccaccat gaattaaata cgattccttt acgcaaattt
     3301 acgtcagtag aaatgaattt attcttctct atagtatcta gaatgcgtga cgtag
//
LOCUS       DAJSBM010000140         3342 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJSBM010000140 DAJSBM010000000
VERSION     DAJSBM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.149"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..2116
                     /locus_tag="ONT67_002813"
     CDS             <1..2116
                     /locus_tag="ONT67_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR2356502.1"
                     /translation="NTIIGENDSGKTNALTALRILLDDSYYYSSKTLKESDFFHGIQN
                     GWQGHWIIISATFEGISEEEFDNEICASISLNSESQTILEELISNADKGVGSISLFIR
                     PNKAIRKQLFDISTELDSHQFNEFRNSIRLSDYEFYYTSKSNLNFCINENYDKLVGRL
                     NDNIASNPEEDDEALLGSRIDMSDIFKHISVVYVDALRDVLREMKNNRNPVKRIMETI
                     ESKISSDNVESLKTIIQQLNETITSVPEIRKIGENINRQLNSIIGSVYSPNLLLSSTM
                     SDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIYLALKIVEFEACRSRELLNIMLIEEP
                     EAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVHLAEVSEISRMNVLKSFNGKSIVMNP
                     ANNLNSFAKEKLKKNNLNLINAVERYLDVKRNGLLFSKGVILVEGDAEEILIPQIVKK
                     LFGINLDELGIGLINVGSTSFEYIASLFDDDRIQRKCAIVTDLDEQAIPSEHRLYKST
                     AQEKGQARKEKLSSLFNENNWVNCFYANTTFEIEFLDVNKENILTYVSPIIDDAFKDK
                     KTITAYKEEIQSEQYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAF
                     ACQEIVDLSIYKKILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDE
                     DVVYLINQIENYTKILYAEVSK"
     gene            2113..>3342
                     /locus_tag="ONT67_002814"
     CDS             2113..>3342
                     /locus_tag="ONT67_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR2356503.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1264 a          448 c          563 g         1067 t
ORIGIN      
        1 aaatactatt ataggtgaga atgattcagg gaaaaccaat gctcttactg cattgagaat
       61 cttattagat gatagttatt actatagtag taaaacacta aaagaatcag atttttttca
      121 tggaattcaa aatgggtggc aagggcattg gattattatc tctgcgacat ttgaaggaat
      181 cagcgaagaa gaatttgaca atgagatttg tgcttccatt tctttaaatt ctgaaagtca
      241 aacaatctta gaagaattga tttcaaatgc agataaaggt gtaggtagta tttctctatt
      301 tattagacct aacaaagcta ttagaaagca attatttgat atttccactg aattagactc
      361 tcatcaattt aacgaattta gaaattctat tcgtctatcc gactatgaat tttactacac
      421 atctaaatca aatctaaatt tctgtatcaa tgaaaattat gataaattgg taggaagact
      481 caatgacaat atagctagta atcctgaaga agacgatgaa gctctgttgg gctcaagaat
      541 tgacatgtca gatattttca agcatatttc ggttgtttat gttgacgctc ttcgagatgt
      601 tcttagagaa atgaaaaaca atcgaaaccc agtcaaaaga atcatggaaa caattgaatc
      661 aaaaatatca tcagataatg ttgagtcatt aaaaacaatt atacagcaat taaatgagac
      721 tattaccagt gttcctgaaa ttaggaaaat tggcgaaaat attaatcggc aactaaatag
      781 cataattggt tcagtgtatt ctcctaatct tcttctttcc tcaactatgt cagatgatat
      841 gggttcttta gcaaaatttc tttcaatgaa gccagaacaa aatatcgact tagacttact
      901 agggttagga catctaaata tgatttattt agctttgaaa atagtcgaat ttgaagcgtg
      961 tcgttcaaga gaactactta atattatgtt gattgaagaa ccagaagctc atattcatca
     1021 tcatattcaa aaaactctgt tcgaaggatt aaatcttcaa aaaaactata ctcagattct
     1081 catgaccaca cattctgtac atttagctga agtgtctgaa atatctcgga tgaatgtatt
     1141 aaaatcgttt aatggcaaat ctatcgttat gaatccagca aataatctga actcatttgc
     1201 taaagaaaag ttaaaaaaga acaatctaaa tctaataaac gcagtagaga gatatttaga
     1261 tgttaaaaga aatggattgc ttttttctaa aggtgttatc ttagttgaag gagatgctga
     1321 agaaatactt attcctcaaa tagtaaaaaa attatttggt ataaacttag acgaacttgg
     1381 aataggtttg attaatgttg gtagtacttc gtttgaatat atcgctagct tgtttgacga
     1441 tgatagaata cagagaaagt gtgctattgt aactgattta gatgaacagg caatcccatc
     1501 agaacatcga ttgtataaaa gcactgcaca agagaaaggt caagcaagaa aagaaaaatt
     1561 atcctccctt tttaatgaaa acaattgggt taattgtttt tatgctaata caacatttga
     1621 aattgagttt ttagatgtga ataaagaaaa tattctaact tatgtcagtc caattataga
     1681 tgacgcattc aaagataaaa aaacaataac tgcttataaa gaagaaatac aaagtgaaca
     1741 atatttagaa agaaatttag cgatgttaaa actagccaat catgttggta aggggtggtt
     1801 tgcaattgag ttaggtgaaa aaattgactt tttaatcaaa attcctaagt atattcttaa
     1861 tgctattgct tttgcatgtc aggaaatagt agatctctca atttataaaa aaattctact
     1921 ccatacaatc gacttgtacg aggaaactac agaattgtca gctttatcag ctagtttaaa
     1981 aacaagtgat acagctgatt tgaatatagc aataatttct ttattagagt actttggtaa
     2041 agatgaggat gttgtgtatc taatcaatca gatagaaaat tacacaaaaa tactttatgc
     2101 tgaggtttca aaatgacaaa tatcttcgaa aaaatcgtaa atgatttaaa tttagaacaa
     2161 aaagatgttg tggaaagtga agataacctt tatgtaaatg catgtcctgg gagtgggaaa
     2221 acaagggtac ttacaaggaa aattgcttac caagcaatta aacacgatgg atcactaaaa
     2281 agaataattg ctatcacata cactaataga gctgctgaag aaattaagga acgcctaagt
     2341 ttacttagta tagatgatga tattaatatt tgggtgggga caatacatca gttttgtctt
     2401 gaatttatta tttatccttt taaaatgaat ctctcaagag tatcaaaagg atttacaatt
     2461 attgatgatt atactcaaag agcttacgtc agccaaatta ataatatgct gaatttaaat
     2521 ctcaaaacgt atgaactgaa caaaattgat ttgtcacttt ctcatgattt taaaattaat
     2581 gaagttcagt atactcaagt ttctgaaatg tatcacacta tgttggaaaa aaataaagaa
     2641 attgattttg atatgatttt actagtagcg tataaaattt taactgtaaa cagtaacgtt
     2701 gcacgtaata ttgctagtaa tataagggcg atttatgtgg atgaatttca agatactaga
     2761 gaattacaat acaatgtaat agctaaatta cttcaaaaca accctcaaat tcagtcaatg
     2821 tttgttggag atattgacca agcaatttat ggatcattag atggtatagc taaatcagtt
     2881 gaagaactag aaatgctaac tagtcacact tttcaatcaa agacgctaca tggctgttac
     2941 aggtctaatc agagattagt agacttctac tctaattttc agagttgtcc ttacgagatt
     3001 gaaagcagag ggaataataa aaacgaccgt ggttttataa gttatgattg caaagttact
     3061 aaggaggatt tacccaacat aataaaaaat ataataaagg aaaagattga aagcggcatt
     3121 catgaaaaag atatttgtgt acttgctcct caacactatc ctctgttttc actgggtgag
     3181 tatttaaaga aagaattacc gtattgcaat tttgatgcta taaatatatc accaattaag
     3241 gtaaataatc atagtttatt tttcaagtta gcaattttgt actttactga atcaggtgaa
     3301 aaggttcgaa gaagaaagct aattgcaaac gatattttaa tt
//
LOCUS       DAJSBM010000141         3303 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJSBM010000141 DAJSBM010000000
VERSION     DAJSBM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.150"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1533)
                     /locus_tag="ONT67_002815"
     CDS             complement(<1..1533)
                     /locus_tag="ONT67_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR2356504.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKI"
     gene            complement(1552..1956)
                     /locus_tag="ONT67_002816"
     CDS             complement(1552..1956)
                     /locus_tag="ONT67_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HCR2356505.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(2188..2445)
                     /locus_tag="ONT67_002817"
     CDS             complement(2188..2445)
                     /locus_tag="ONT67_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR2356506.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(2570..2902)
                     /locus_tag="ONT67_002818"
     CDS             complement(2570..2902)
                     /locus_tag="ONT67_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356507.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         1040 a          601 c          440 g         1222 t
ORIGIN      
        1 aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt gaacttctcg
       61 actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg atgttaattg
      121 gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat aatagtcaaa
      181 ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa tctcttgata
      241 ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc taaaatatct
      301 atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt gataaagata
      361 ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat ccatttgaat
      421 atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca cccattgtgg
      481 taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa aggcattggc
      541 acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc gatagtttaa
      601 taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt cttcttctga
      661 tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt ctaatgttgg
      721 aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat cctcaggtgg
      781 ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat tatcacccat
      841 ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt cattgctcaa
      901 tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta aaccctttct
      961 tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg acaagaaaag
     1021 ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct tctcttctat
     1081 ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt ttccagttcc
     1141 agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg ctgctttaat
     1201 ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta ttccaaccct
     1261 aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt gttgatttgg
     1321 aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc caaattctcc
     1381 atcttttacg aattcattca aatattgaga gacaatatta cgactaacat ttaatttttc
     1441 agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt tctctatgac
     1501 ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac actattcgaa
     1561 ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt ttagatcatt
     1621 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggcga
     1681 aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg attcttcatc
     1741 tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt cctctaagga
     1801 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     1861 cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     1921 cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct atctcttatt
     1981 taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag caaaaaaata
     2041 aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa aaatagccat
     2101 atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa tcacagtagg
     2161 tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg tgattttctc
     2221 aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa gaaagcaatc
     2281 caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt gttgttgaaa
     2341 gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag tcaattcatt
     2401 taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag cttcctgttt
     2461 ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt cgattgctga
     2521 ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt caagagaact
     2581 tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt acctgccacc
     2641 taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc tccaacattg
     2701 taagtactgt tggtgttaat atagggactt tgtacaattg tatttttttg attttatcat
     2761 ctgcacaaag gagatttttt aaatctaatc cttcttttat ttcaaaagtt gaatttttta
     2821 aatctataag agtaaaacca attccttcat actgaatttc tgaaaatcga atttttctcc
     2881 cagttgatag atcaaggggc agaaaacctt ttattacatt gtgagtgtca ccatcattta
     2941 caatgtcatt aggaattttg attttagaat tctgtggagc acctattgga agataataat
     3001 tcattataat tttgtagtct tcatgaggaa ataaagtact tttaggattg ctaagatatt
     3061 ctcgtaccga atctaagaat ggcttatcca ttgaatcttc atccccattt aaactaaaaa
     3121 acattcctac aattgctttt gcaactctaa gtgaattcat tttctttatt gaatgtgtta
     3181 aatcagactt accattcttt aaacttgtat tgttataaag gaaattttta ttgtcttctg
     3241 caaatctaat atattcctca acatactcac taccaaaaaa actattacat ttactacata
     3301 acg
//
LOCUS       DAJSBM010000142         3206 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJSBM010000142 DAJSBM010000000
VERSION     DAJSBM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.151"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            12..3161
                     /locus_tag="ONT67_002819"
     CDS             12..3161
                     /locus_tag="ONT67_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCR2356508.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1130 a          543 c          688 g          845 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgatttccc acgaatcgct attacctatt
     1921 cattagaaga aaatagccga gattcaagtg cccaacaaga agaaatgcaa aaaattattg
     1981 aggaatataa tcagtattat gatactgcct ggtcactagc agacattgag cgttataacg
     2041 gagacatcaa taatcgtttg gcacgaaaaa gagcggaatt caaacaattt gggaaacaaa
     2101 tcgatttagt aatcgtagtg gatcgtctat tgacaggttt tgacgcccct acgattcaaa
     2161 ccctatttgt ggatcgtaat ctagagtatg caggattgat ccaagccttt tcacgtacga
     2221 accgtacgta tcctgaaaaa acaaaaggac taattgttac tttccgaaag ccagcgacaa
     2281 tggagaagaa tgtcgaggat gcgacaaaac tgtattcaga agctaaagaa gaatcgggtt
     2341 tgatttatcc aagttaccaa gaatccaaaa aaagattcaa gcaagcatat ggtaagttaa
     2401 aagaggtttc atccatggaa tccattgatg aacatacgcc acttgaaatc cgtatcgagt
     2461 acgtcaaagc atttcaagaa ttgaatcgtg cgtatgaatc gttagtgact tatgatgatt
     2521 acaatgatga catggtgcaa tcttcaactt tgaacaatca aattcaactt ttggaagaac
     2581 aaattggcgt gtacgagacc gtgaagggtt cattgatgga ggaagaacca gaaaaagagg
     2641 gagaagattt ttcaacgatc gaattttata gtgaaaatac cactaagttg tatgatattg
     2701 attctactta tattgatcaa ttactaggaa cttatgctgc gaatagttca gatgcacgag
     2761 aagaaattga aaaagctttg gctaaattga ataaaacaga aggagtcaaa gaagtttacc
     2821 gacaaatttt aaatgcattt gatgtgggga ctttagatag taatgaagat atctttgtca
     2881 ttaaacgtcg ctattttaca caagcaagtg ataatttgat tcatgccttt tccaatgagt
     2941 ggtttgtttc tgagaaagaa ttacatgctt ctaatctgca atatatgcca ggtgaagatc
     3001 caattcctaa tatgaaagca attatcaaca gtaaggatta cgaaggatat aaagccaaac
     3061 atccagaagc gaaaccattt aaatacccac aagaaatgaa gcgagcatgg cgtaaaatgt
     3121 tagatgatga gttgattcca cttgaaaatg aattgaggta aacgagaaga gttaagaaaa
     3181 ttgtacgatt caaagatagt agtaaa
//
LOCUS       DAJSBM010000143         3179 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJSBM010000143 DAJSBM010000000
VERSION     DAJSBM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.152"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..651
                     /locus_tag="ONT67_002820"
                     /pseudo
     CDS             1..651
                     /locus_tag="ONT67_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            713..1330
                     /locus_tag="ONT67_002821"
     CDS             713..1330
                     /locus_tag="ONT67_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356509.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            2350..>3179
                     /locus_tag="ONT67_002822"
     CDS             2350..>3179
                     /locus_tag="ONT67_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2356510.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         1138 a          454 c          545 g         1042 t
ORIGIN      
        1 aaagacgtca ttattgtcgc tgttggttac tacctgcgtt acaatctaag ctatcgtgaa
       61 gttcaggaat tgttatatga tcgtggaata aatgtttgtc atactacgat ttatcgttgg
      121 gtataagagt acagcaaagt cctctattat ctttggaaga agaaaaatag acaatccttc
      181 tattcatgga aaatggacga aacctatatc aaaattaagg gacgttggca ttatctttat
      241 cgtgcaattg atgcggacgg cttaacctta gatatctggt tacgaaagaa acgggatacg
      301 caagcagcct atgctttctt aaaacgactc cataaacagt ttggtgagcc gaaagcaatt
      361 gtgaccgata aagcaacttc tcttggctcc gcctttagaa agttacagag tgtgggttta
      421 tatactaaga cagagcaccg aactgtgaag tatcttaaca atttaataga acaagaccat
      481 cgacctatta aacgacgaaa taaattttat caaagtctcc gtacagcctc ttccacgatt
      541 aagggcatgg agaccattcg aggaatatat aaaaagaacc gaagaaatgg aacgctcttc
      601 ggcttttcgg tgtctactga aatcaaggta ttaatgggaa taacagccta agatatttgg
      661 agttcagaga gggcgcgttt gattttcaaa cttcgcaaca gaaccgagaa ccttgggttt
      721 tcaagtgctt tttggttcat taatcttaac catagtgttt ttaaagtgga aaacaagcaa
      781 caaaaaaagt atgtggagcc ttgtaggaat agttcttatt atttttactg tgactgcagc
      841 aacagaagca aattattcgc ataaaattca gtcgtataaa ggtcgtttag ttatgaccaa
      901 aaaagatgtt acacgttata catcctcaag aactggaggc ctatatgact atgcaccatt
      961 agatgcattg aaatataaag atcatttaaa gaaacatgaa gtaaaagttg gtgatcggtg
     1021 ggagaatgca tctcataaag catataattc caaaatagtc tatacagtag aagatgccga
     1081 tgagcaaaat atcacattac ctgtattcaa ttattgggga acagttgtga aagtaaatgg
     1141 gaaaaagagc actatagaaa atgacaatgg tttagtgtct attaaaggta cggggaaaag
     1201 aactatagtt gaggtttcat ctacgtatcc aactacgatg attctatctt tattaattag
     1261 ttcaataact tttatattct tatttacacg ttatttaata agatgctgcg gtgaaatgaa
     1321 aatagattag atagatatta taaatatgtt gattctattt tcatagcaat ggagaaaata
     1381 cattagcaat atataaaaga aggtatcaag agtcacagtg aatttattcc gaaaagtgaa
     1441 aagagcaaat ataaaattta atgaaaatat agaactaaat aggaaaacgt agacgtaatt
     1501 ttctacgttt tccttttcat atatattaaa tattacggaa aattatataa actaatttag
     1561 tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg tctatttaac
     1621 tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc gtacttaatt
     1681 ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt atgatatata
     1741 tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa
     1801 aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga
     1861 tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc
     1921 aaaattatgt gacaaaaaaa actagtagaa gaaagaataa tggcttgata ataaagtact
     1981 acctattaaa ggtagtactt tatttaattt agtataaatt aacatactac tatattttag
     2041 gtttcatatt atataaatta ataatatact accatttgtt ggtagtataa tttaaaggta
     2101 ataagttatt cttttattac tatgtttggg ttttatagca aagaaagttt aataagagtt
     2161 tggacctagc aaacaatatt aatgaggtca ttaaatctta ttatctttag tgatataacg
     2221 aaatatctac acagacaaaa tgatagcgta aaatattttg tgtaaataga tatttagggt
     2281 agaaaaaaag aaagtccatt ctgtaaaatt aaagttacca caaccgaaat tttataggag
     2341 gactttctca tgcccaattt tactacagaa attatggaaa cactaatcaa taaaggtgat
     2401 ttagatgaat tatttcgatg tcatttagaa ttagctgtta attcattatt gcaggctgaa
     2461 ttaacagctt ttcttgatta tgaaaaatat gatcgtgctg ggttcaattc aggtaattcc
     2521 cgtaatggga attattcacg ttcattcaga acagagtatg gagaattaaa tttggtaatt
     2581 cctagagata gaaatggtaa gttttctcaa caaactttac ctgcttataa aagaaccaat
     2641 gactctttag aaaccactat tattcagcta tttcaaaaag gaattactat gtctgaaatc
     2701 tctgaattga ttgaaaaaat gtacgggcat cactatacgc cacaaaccat ttcaaatatg
     2761 actaaaatag tttctgaaga tattattgcc tttaaagaaa ggtccttaga atccagatac
     2821 tctgtcattt ttatggatgc tactcacatt cctttaaaaa gacaaactgt ttcaaaagaa
     2881 gcagtttata ttgtaattgg cattcgatta gatggaacca aagaggttct tgggtttagt
     2941 cttgctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagacct aaaagaccgt
     3001 ggtttaaaag aggttttatt agtcgtaact gatggtttaa gtggcattaa cgatagtatt
     3061 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
     3121 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa attagttta
//
LOCUS       DAJSBM010000144         3172 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJSBM010000144 DAJSBM010000000
VERSION     DAJSBM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.153"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(389..1003)
                     /locus_tag="ONT67_002823"
     CDS             complement(389..1003)
                     /locus_tag="ONT67_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR2356511.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1609..1986)
                     /locus_tag="ONT67_002824"
     CDS             complement(1609..1986)
                     /locus_tag="ONT67_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356512.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2286..3167)
                     /gene="galU"
                     /locus_tag="ONT67_002825"
     CDS             complement(2286..3167)
                     /gene="galU"
                     /locus_tag="ONT67_002825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR2356513.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          965 a          656 c          490 g         1061 t
ORIGIN      
        1 ttatatcagg taaacttcat tgcaataatg ataacaaatg tactataatt actttgtaaa
       61 gagttttggg gaagactctt tcatcaacgt gtattaagag acaagaaaga atccatatgg
      121 attctttctt gtctcttttt ccactacttt ttatttttct gacactacat attcttataa
      181 ctcatttctc ataactgtga aacaacttat aaaaataaca aactttaaac aaaacaagct
      241 taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc gttgttaatc
      301 ctcattaata atcgttttct ttttttcaaa cgctccattt ttatgtggaa cattattttt
      361 ttaaggaact ttgtcaacat gtggataact attttccact tgtgtcctgg actagctgtt
      421 ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt tgcaagagag gctgttccgc
      481 agatcgctgg atctagattt ggcaaagtca agactgcatc atgtaaattg gcgtggttgt
      541 ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact gctatgttgc gatttgatac
      601 ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg accattttct ttgatccact
      661 gagctagttc gatgattgcc gggtttcttc cttcattcga aagaagaaga aaaatgtctt
      721 ctgatttgac agaaagctga tcaataattg cctgtcctgc accttctagc gtttcataga
      781 cacctccggc tggattttca atcaatatac ttggtacaag atccggggtt tgatcccaaa
      841 attcaaatgc tacaccactt agtgtgcggc tagcatatat ctgcaatcta ccaccttgat
      901 taatttgttt cgcgatccaa gtgccagctt gaagcatcag cggctcttct atccttaatt
      961 tctcttccaa ttggtgataa tagtcaaaat aacataaact catttatctc tcccgcctag
     1021 ctatacttga catgaataga aacagctgcc taatctaagg aagtacataa aaaaacagta
     1081 gttagacagt aacacatgtg gtctttgctg tttcaaacac aaaaaaggca ttgaacgaat
     1141 acgcccaata ccttgacata gcaaataaaa caacgtgcag accatggaaa atatctacat
     1201 aggggtagaa ttttcaccct agattaggta caataaatta ggtcgatgca ctgcattgac
     1261 tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg tcattccctg attcttagcg
     1321 ccaactaagg gtcagggagc ctttttttat tcagctgttt ctatctgaat ttattgtaag
     1381 taatctctta tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg atttatatat
     1441 atataaataa cataaaaaaa ggaatggctt atcccgatac aacaagcatt cctttaaaaa
     1501 agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat ctctcatctc
     1561 aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt accgtcttct
     1621 tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg tatgtctacc
     1681 ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta gatacagtac
     1741 agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt caaccatcaa
     1801 ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt ctttttttct
     1861 gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa atatccaatt
     1921 gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag cgactaattt
     1981 tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt attctactat
     2041 gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat acaacttaaa
     2101 aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc tttttctatc
     2161 tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca gttgcgcaaa
     2221 tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag gccacttatt
     2281 tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc acttaatgaa
     2341 gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata tcctaactta
     2401 tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc tgtctggtta
     2461 agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc ttgagtttct
     2521 aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa attacttggt
     2581 gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc cttttcgcta
     2641 tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac acgcatgacg
     2701 gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt caatgggata
     2761 tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc tataaatgct
     2821 tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat gaagtgaaga
     2881 ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt ttcccgcaaa
     2941 ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt ttcttttccc
     3001 gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat aaactggatc
     3061 gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc cggcaaaaaa
     3121 cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcacttg ct
//
LOCUS       DAJSBM010000145         3128 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJSBM010000145 DAJSBM010000000
VERSION     DAJSBM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.154"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            103..1347
                     /locus_tag="ONT67_002826"
                     /pseudo
     CDS             103..1347
                     /locus_tag="ONT67_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="ONT67_002827"
     CDS             1360..1992
                     /locus_tag="ONT67_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCR2356514.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="ONT67_002828"
     CDS             2197..2424
                     /locus_tag="ONT67_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR2356515.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="ONT67_002829"
     CDS             2493..2759
                     /locus_tag="ONT67_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR2356516.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="ONT67_002830"
     CDS             2749..3018
                     /locus_tag="ONT67_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2356517.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DAJSBM010000146         3114 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJSBM010000146 DAJSBM010000000
VERSION     DAJSBM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.155"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(1..940)
                     /locus_tag="ONT67_002831"
                     /pseudo
     CDS             complement(1..940)
                     /locus_tag="ONT67_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(1161..1523)
                     /locus_tag="ONT67_002832"
     CDS             complement(1161..1523)
                     /locus_tag="ONT67_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356518.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(1527..1865)
                     /locus_tag="ONT67_002833"
     CDS             complement(1527..1865)
                     /locus_tag="ONT67_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356519.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(1844..2263)
                     /locus_tag="ONT67_002834"
     CDS             complement(1844..2263)
                     /locus_tag="ONT67_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356520.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(2256..2489)
                     /locus_tag="ONT67_002835"
     CDS             complement(2256..2489)
                     /locus_tag="ONT67_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356521.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(2486..>3114)
                     /locus_tag="ONT67_002836"
     CDS             complement(2486..>3114)
                     /locus_tag="ONT67_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2356522.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT          868 a          701 c          433 g         1112 t
ORIGIN      
        1 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
       61 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
      121 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
      181 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
      241 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
      301 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
      361 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
      421 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
      481 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
      541 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
      601 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
      661 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
      721 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
      781 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
      841 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
      901 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
      961 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     1021 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     1081 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     1141 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     1201 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     1261 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     1321 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     1381 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     1441 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     1501 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     1561 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     1621 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     1681 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     1741 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     1801 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     1861 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     1921 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     1981 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     2041 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     2101 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     2161 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     2221 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     2281 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     2341 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     2401 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     2461 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     2521 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     2581 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     2641 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     2701 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     2761 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     2821 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     2881 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     2941 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     3001 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     3061 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaa
//
LOCUS       DAJSBM010000147         3087 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJSBM010000147 DAJSBM010000000
VERSION     DAJSBM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.156"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..162)
                     /locus_tag="ONT67_002837"
     CDS             complement(25..162)
                     /locus_tag="ONT67_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCR2356523.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="ONT67_002838"
     CDS             complement(159..1343)
                     /locus_tag="ONT67_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCR2356524.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1602..1892)
                     /locus_tag="ONT67_002839"
     CDS             complement(1602..1892)
                     /locus_tag="ONT67_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356525.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT67_002840"
                     /pseudo
     CDS             complement(1882..3024)
                     /gene="dcm"
                     /locus_tag="ONT67_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT          842 a          739 c          540 g          966 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaagcaca
      961 cctttatctt cttcatgaga caacatcacc acaatatcac ccatgacata ctgttcttga
     1021 taggagtaaa aggcataatc ttcatggagc atatactcaa atttcaaatg gagaatttct
     1081 tgaataattt ttcgtgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gtcaaaggca aatcgggatt aaagctttcc agttggtcaa aaattttcct catggttttt
     1201 atagatgcgg ttacgccacc attttctatc tgatttaagt acggccgact aagtccacaa
     1261 gccgtagcca atttattttg tgaaacgccg tattcgttgc gtcggtcttt taatagccga
     1321 taatctaaat tccgttgtgc caagttctca cctccaaatt caaggtgtta actttttcaa
     1381 atgagttaac acctatacaa attacttact atctttgata caacaagcat tcttaatggt
     1441 tgcttactta ttttttgtgt ttttgtaccc ccctgttaga tacgggggtg agacatcatg
     1501 gtcggctaac gccgtccatg aacaggcaca gcaccacgga ggatttctgc ttcgcgaatc
     1561 ctccgcggtc agcgcctaac agtcagttgt gttggtttag gttactcatc tgtaatacca
     1621 tttacttcca actctgcttt catttctaac aagtattgac gaatatctgg ttcttttgct
     1681 tggtccgcaa tccgctcaaa ttctgcaatt acttgattta tttcaattcg agcatccgtc
     1741 tcctcattca ttaaatatgg atcgccagcg actttgacac cattcttgcg aatatcaaat
     1801 tttaaccgtc gcaattgttc ttctaattct gggtctgact gaatctttcc aatcacccaa
     1861 taaatttctc caatcaactc atcataaacc atgtacatcc aactctttta atcttctggc
     1921 aatatcaaaa atgaccggaa ctgttacaga gtttccggct tgcttataca actgactatc
     1981 tgaattgact tgagaatctc gatcaaaagc ccaatcggga aagccctgaa gtcgccaaca
     2041 ctctctaggt gttagcttcc gaatttgata ctgatccgtg acgatcgctt gttggtctcc
     2101 tgttagtaag gtatgaacac tctgttttcc cagacgacct cggcgacttg tggaattagg
     2161 aaaagccaga ttcacactgt cttctggctt cacctcttga tattctgttt cattttttcg
     2221 aatcatgatt tttgtaccac cttgtttcgt tgacaaggtg ggggcggttc cagtaacttc
     2281 ataaactcga ttaccttgtt caaactttcc cggtaatagt cctgctacag agattccatc
     2341 ccttgctggc agtgcgattt tgattccttc atcacctgtt aaggttggcg ctacgccatc
     2401 tgaaccatag acttgtccac ccattcctcg attggaagga ttgatattta ccaaatttct
     2461 gatgttttga tcagatgttg aagacggtcc aatgatagga aatacttgtt cggtacatgc
     2521 gtctcgagaa tgtccgataa ggaatatccg ctcccggctt tggggaatgt aggcctttga
     2581 gttaagcacc taccactcca cgtcataccc caatccatcc agcgttcgga ggatcgtctc
     2641 gaacgtagcc cctccttcgt gattgagcaa tcctcggacg ttctcaagga ataaaagctt
     2701 aggtcggaga agagtggtga accgagcgat ttcaaagaat aaagtacctc gagtatcgga
     2761 aaatctttgc cgctttcccg caattgaaaa agcttggcac ggaaatccgc cgcaaaggat
     2821 gtccactggt ccgagggatt gaataaactc atctgataca cttgtgatgt catgcatttc
     2881 cacctcctta cttgtatcat gaattgcttt ataactacgt ctagcaaatt tatcaatttc
     2941 acaaaaaccg atacaatgat ggctcgcctg ttccatacct aaacgaaagc caccaatacc
     3001 ggcgaataaa tctaaaaatc tcatacatta cctcattcat tgcaaagaaa aaagccaaga
     3061 acattttgct cttggctcat acattat
//
LOCUS       DAJSBM010000148         3039 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJSBM010000148 DAJSBM010000000
VERSION     DAJSBM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.157"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            193..807
                     /locus_tag="ONT67_002841"
     CDS             193..807
                     /locus_tag="ONT67_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356526.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(972..1796)
                     /locus_tag="ONT67_002842"
     CDS             complement(972..1796)
                     /locus_tag="ONT67_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356527.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(1820..1975)
                     /locus_tag="ONT67_002843"
     CDS             complement(1820..1975)
                     /locus_tag="ONT67_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356528.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(2434..2691)
                     /locus_tag="ONT67_002844"
     CDS             complement(2434..2691)
                     /locus_tag="ONT67_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCR2356529.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2684..2953)
                     /locus_tag="ONT67_002845"
     CDS             complement(2684..2953)
                     /locus_tag="ONT67_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCR2356530.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1016 a          519 c          399 g         1105 t
ORIGIN      
        1 aatcttaaca gataaatcaa gttatatact agaaaattca taaaatatat ttttaatgtc
       61 ataatcgttt aaattttctt gattgacatt tatttacaaa aaatattgcc atttaaaaag
      121 aaaagtgaca tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
      181 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
      241 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
      301 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
      361 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
      421 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
      481 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
      541 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
      601 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
      661 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
      721 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
      781 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
      841 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
      901 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
      961 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     1021 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     1081 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     1141 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     1201 tacataaagc tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa
     1261 cattcaaaac attcttctat agttggttta cttccttgag gagaatgaac caatttatta
     1321 cgtttttcta tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag
     1381 taccctttaa acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat
     1441 attagagtta aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct
     1501 gtaattatcg aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt
     1561 ataagagaaa aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca
     1621 gaagttatat ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa
     1681 atttctatcc aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca
     1741 gcaagcatcg ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca
     1801 gcaatattgc taaaatcagc taatttttct ttagaataaa aaaaacattt atatcagaat
     1861 caataaattc tatttttttt attactatat cgtcatcatt atcttgacca tttatgttta
     1921 ttgttccctg agtggggagt acttctttca aattcaagtt aaatttatat tccattactt
     1981 ctcctccaat atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt
     2041 aatttttagc taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac
     2101 tagtacaaaa aatttaaaat agattattaa acctattgct tattctattc agtcatatat
     2161 ataagcgtca tcctttagta actaacatcc cacactacaa tagtattata acatatctat
     2221 tcaacttcaa attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat
     2281 gattttacta accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa
     2341 cactaagcta tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt
     2401 taagtttctg accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat
     2461 aaatatcgtt tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga
     2521 ccattttcca gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga
     2581 acgatcgata tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc
     2641 atgccaataa agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa
     2701 cctcgattaa gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa
     2761 ttttttccat gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat
     2821 ctctttttga taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt
     2881 cagcatcctc attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag
     2941 ctactgcttc cattcctttc accccttatt tatttctata aaacaattgt acattatatt
     3001 gtacaattaa gcaaatgatt aatattctaa cttcttata
//
LOCUS       DAJSBM010000149         3002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJSBM010000149 DAJSBM010000000
VERSION     DAJSBM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.158"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1404
                     /locus_tag="ONT67_002846"
     CDS             <1..1404
                     /locus_tag="ONT67_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCR2356531.1"
                     /translation="VEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTP
                     GNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTN
                     LPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADK
                     YNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPD
                     GSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWK
                     EKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKT
                     EPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVV
                     SSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLIL
                     LGTLGLLGYKNRKKENE"
     gene            1538..2881
                     /locus_tag="ONT67_002847"
     CDS             1538..2881
                     /locus_tag="ONT67_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR2356532.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1192 a          403 c          578 g          829 t
ORIGIN      
        1 gtagaaggcg aaaaagtaga agttgggggc acagtagatc taacagataa tgttactaac
       61 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
      121 accaacacac caggtaatta cgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      181 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctgacgccga taaatatgaa
      241 ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag tagatttaac agataatgtt
      301 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
      361 attgatacca acacaccagg caattatgaa ggtgtcattg aagtaacgta tccagacggt
      421 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctaa tgcagataaa
      481 tacaatcctg aaggacaaaa agtgactaca gacttaaata aagagccaga tgcatctgag
      541 ggaattaaga acaaagaaga tttaccaaaa ggaactactt atgcttggaa agagaaagta
      601 gatgttagca cagctggaaa taaaaaaggt actattgtag tgacatatcc agatggatca
      661 tctgatgaag ttgaagtaga cgttacagtg acagatactc gttccgatgc agataaatat
      721 aatcctgaag gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga
      781 attaagaaca aagaagatct accaaaagga actacttata cttggaaaga aaaagtagat
      841 gttagtacag ctggaaacaa aaaaggtact gtagtagtaa catatccaga tggatctaaa
      901 gaagaagtag aagttactat ttctgtagta gataaaaaag caccgaataa acctcaagtt
      961 gatcctatta cagagggtga ccaaattgtt actggtaaaa ctgaaccaaa tgcagaggtg
     1021 acagttacat taccagacgg aagtcaacac catgggacag ctgataaaaa tggtaatttt
     1081 acagttaaag ttcctaaatt agaggcaggg acaaaagtga tagtaactgc aactgatgaa
     1141 tctggtaata ctagtgaacc tactaatgta gttgtttctt ccaacaaaaa agacagtgga
     1201 aaaaacggta gtaaaggtag taagactgac aatcaaggta gcaacagcaa tcaagacaaa
     1261 aatagaggca aaagccagtc ctcaaaagtt tttcctaaga cgggagagag tgacagtaat
     1321 atctttacta ttagcggtgg tttaatactg ttaggaacgt taggattatt agggtataaa
     1381 aatcgaaaaa aagagaatga atagaaaatc aatttcttaa gtcttctctg agaagaggaa
     1441 gaatcttttt cttcctcttt ttgctttttt atgatgggaa ataagtgtta gttaaagatg
     1501 aatatacttt ttatagaaag gaaaagaatg taattaagtg gaaaaaatag taattattgg
     1561 ttcaaaccat tcagggctag ccgcagctcg atttctaaac ttaagcgatt caaattatga
     1621 agttattcta atcgataaaa aatcagaaat tagttacttg gaatgtggga catcgttatt
     1681 tataaaagga gaagtaagta atttagaaga tcttttttat caaaatagaa tgacactaaa
     1741 ttctgagata aaaaagttat ttctagagac agaagttgac aaaattgata ttcaaaataa
     1801 aaagattagt tgtgtaaaaa aagatggtac aagattctct caagaatatg acaaagttat
     1861 attagctaca ggttcaaagc aaattatgct agatataccg gggaataatt tagctaatat
     1921 tttttcgata aagaattttc caaatgcaaa agagattttt aaagctttgg atgatagtac
     1981 tattaaaaaa gtaggaatta ttggagcagg atatattgga gttgaaattg ctgaagccat
     2041 aagaaaaaga gggaaagaag tatatctttt cgatgttgca gatagagtgt tgtcgactta
     2101 ttacgaccgt agtttttcag ataaagttga agaaatacta tcaaaaaatg gaatacatct
     2161 ttgtctatca gaaagtccta aaaaatattt aggaagaaaa aagattgaaa aaatagtcac
     2221 tacaagtggt acccaatact ctatggatat ggtcgtacaa gctataggat ttctatctaa
     2281 tagtccaatt ggggaaaagg aactcttgag agatgttagt ggagcatatc taactaatga
     2341 ataccagcaa acaaatataa aagatatata tgctattggg gattgtgcta caactttttt
     2401 tacgtccatt aatagaagta gtgttgattt ttctatttct aatgctctaa gaacagctta
     2461 tattgcaact caacatatta acaatcaaga ttttacacct tctggttccc aatttacaaa
     2521 tggcttttct atatttaatt acaattttta cgcagcaggt ttgaatttaa aaacagcacg
     2581 attattaagt gagtctatag attatataga aatagaagat ttcattagac ctgctttttt
     2641 gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa aaaaatacaa agagaatagt
     2701 aggtgtacaa ctttgtagtc aagaagattt atctggacta ttatcaatgt tttctcttgc
     2761 tatagaagaa caaattacat tagacaaatt gaaaatgtta gattatttat ttcatcctag
     2821 ttttagccaa ccctataatt ttattttaaa ggcagtaaca ggattaaaca ttgaaacata
     2881 atcgttacta aatgaaatca agaaatatac tgtattactc aattcattga aaatatttat
     2941 cataattttt aatagaaaac actattaaag agaaaaaaga gaggtaaatc aaatgaaaaa
     3001 at
//
LOCUS       DAJSBM010000150         2845 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJSBM010000150 DAJSBM010000000
VERSION     DAJSBM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.159"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            802..1056
                     /locus_tag="ONT67_002848"
                     /pseudo
     CDS             802..1056
                     /locus_tag="ONT67_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1126..1638
                     /locus_tag="ONT67_002849"
     CDS             1126..1638
                     /locus_tag="ONT67_002849"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356533.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="ONT67_002850"
     CDS             1635..2672
                     /locus_tag="ONT67_002850"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2356534.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1026 a          502 c          567 g          750 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac cgaccatcgt ccagt
//
LOCUS       DAJSBM010000151         2820 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJSBM010000151 DAJSBM010000000
VERSION     DAJSBM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.160"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..936)
                     /locus_tag="ONT67_002851"
     CDS             complement(61..936)
                     /locus_tag="ONT67_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2356535.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(1191..1275)
                     /locus_tag="ONT67_002852"
     tRNA            complement(1191..1275)
                     /locus_tag="ONT67_002852"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1239..1241),aa:Leu,seq:caa)
     gene            complement(1293..1364)
                     /locus_tag="ONT67_002853"
     tRNA            complement(1293..1364)
                     /locus_tag="ONT67_002853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1330..1332),aa:Gln,seq:ttg)
     gene            complement(1374..1446)
                     /locus_tag="ONT67_002854"
     tRNA            complement(1374..1446)
                     /locus_tag="ONT67_002854"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1411..1413),aa:His,seq:gtg)
     gene            complement(1508..1592)
                     /locus_tag="ONT67_002855"
     tRNA            complement(1508..1592)
                     /locus_tag="ONT67_002855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            complement(1613..1683)
                     /locus_tag="ONT67_002856"
     tRNA            complement(1613..1683)
                     /locus_tag="ONT67_002856"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Cys,seq:gca)
     gene            complement(1729..1800)
                     /locus_tag="ONT67_002857"
     tRNA            complement(1729..1800)
                     /locus_tag="ONT67_002857"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1766..1768),aa:Gln,seq:ttg)
     gene            complement(1812..1884)
                     /locus_tag="ONT67_002858"
     tRNA            complement(1812..1884)
                     /locus_tag="ONT67_002858"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1849..1851),aa:His,seq:gtg)
     gene            complement(1887..1960)
                     /locus_tag="ONT67_002859"
     tRNA            complement(1887..1960)
                     /locus_tag="ONT67_002859"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Trp,seq:cca)
     gene            complement(1967..2047)
                     /locus_tag="ONT67_002860"
     tRNA            complement(1967..2047)
                     /locus_tag="ONT67_002860"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2011..2013),aa:Tyr,seq:gta)
     gene            complement(2049..2124)
                     /locus_tag="ONT67_002861"
     tRNA            complement(2049..2124)
                     /locus_tag="ONT67_002861"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2089..2091),aa:Phe,seq:gaa)
     gene            complement(2160..2232)
                     /locus_tag="ONT67_002862"
     tRNA            complement(2160..2232)
                     /locus_tag="ONT67_002862"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2197..2199),aa:Asp,seq:gtc)
     gene            complement(2242..2314)
                     /locus_tag="ONT67_002863"
     tRNA            complement(2242..2314)
                     /locus_tag="ONT67_002863"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2279..2281),aa:Val,seq:tac)
     gene            complement(2330..2401)
                     /locus_tag="ONT67_002864"
     tRNA            complement(2330..2401)
                     /locus_tag="ONT67_002864"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2366..2368),aa:Glu,seq:ttc)
     gene            complement(2416..2505)
                     /locus_tag="ONT67_002865"
     tRNA            complement(2416..2505)
                     /locus_tag="ONT67_002865"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2469..2471),aa:Ser,seq:gga)
     gene            complement(2545..2618)
                     /locus_tag="ONT67_002866"
     tRNA            complement(2545..2618)
                     /locus_tag="ONT67_002866"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2582..2584),aa:Asn,seq:gtt)
     gene            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT67_002867"
     rRNA            complement(2628..2743)
                     /gene="rrf"
                     /locus_tag="ONT67_002867"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          829 a          652 c          536 g          803 t
ORIGIN      
        1 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
       61 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
      121 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
      181 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
      241 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
      301 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
      361 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
      421 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
      481 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
      541 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
      601 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
      661 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
      721 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
      781 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
      841 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
      901 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
      961 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
     1021 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
     1081 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
     1141 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
     1201 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
     1261 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
     1321 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
     1381 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
     1441 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
     1501 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
     1561 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
     1621 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
     1681 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
     1741 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
     1801 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
     1861 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
     1921 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
     1981 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
     2041 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
     2101 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
     2161 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
     2221 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
     2281 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
     2341 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
     2401 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
     2461 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
     2521 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
     2581 taacagtcat gcgctactac caactgagct atgccggaat aacatcagcg tggcgacgtc
     2641 ctactctcac aaggggcaac ccctcactac aatcggcgct aagaagctta acttctgtgt
     2701 tcggcatggt tacaggtgta tccttctcgc tatcgccacc acactgtggt gttatctttt
     2761 attgagtaaa ttttgttcac tcaaaactgg atttgaagtc acataagttt ttttccgagt
//
LOCUS       DAJSBM010000152         2744 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJSBM010000152 DAJSBM010000000
VERSION     DAJSBM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.161"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..560)
                     /locus_tag="ONT67_002868"
     CDS             complement(<1..560)
                     /locus_tag="ONT67_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356536.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFV"
     gene            complement(1274..1564)
                     /locus_tag="ONT67_002869"
     CDS             complement(1274..1564)
                     /locus_tag="ONT67_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356537.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1772..2464)
                     /locus_tag="ONT67_002870"
     CDS             complement(1772..2464)
                     /locus_tag="ONT67_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2356538.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          828 a          503 c          385 g         1028 t
ORIGIN      
        1 acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa aggaatactt
       61 acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa cggataaaaa
      121 gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct ccaaccggtt
      181 tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa taaattttta
      241 acatttattt tctcaaaaac atacttgcaa agtaatacta caattcctaa cataatcgta
      301 gaatttaata atttccatag cataaagtgt ttagaaaaga acattaagaa tagttctata
      361 ataattcttg aactccatgt cttataatgt tctatcgaag cacccaaata gccaagttca
      421 tctaatctac ttgcataaac tacgtcatcc gctctcatag tcggcatata catattaaag
      481 agtaaaaaga taccccaaat ccctattagt aacaagatca gttctttgtt attcgttatt
      541 tttctattat gtttgttcat ttttatactc cttcttatat ttcctaatag agtataacat
      601 agctttaagc attacttaaa gataacttaa aagttatctt ttaaatactt aattgttaaa
      661 taccttattt gatgcaaaag aaatctacga tagtcaacaa aaaaattttc attatatcca
      721 gaaaaatttt tcttagttaa ggacctatgg attgcagtaa tgcagaattc gtcgaataaa
      781 ctgccaaaga catacaatca tattgctttt aaaattgacg aacaagatat agattcgttt
      841 gtctcaaaaa tccaaatgtt aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc
      901 gaggcttctt caatttactt ttacgactat gataatcacc tcttcgagtt acataccggt
      961 actctccagg aacgtttaaa atcctacaat tccactacct gagactagta atgactagtc
     1021 tctttttatg gcctctgatc cccccattga gcgaatacta atagggcttc gaattgatct
     1081 gttcaatctt taagcaatat ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa
     1141 tacttaaata catgtagtta tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg
     1201 ttcaaatccc atcgtctgca tccatctttc caaaacaagc ggctaggaca tttttgtctc
     1261 agccgcttgt tttctagttt attctttcat cttgtcggac cttttttctt gggtatagac
     1321 ttttctcata ttctaccaca attttgggtt tccctaacac atgataggat ctcacaacaa
     1381 attgctttct attattataa taacctgtaa cgactaactt catcccttcg tctacttcag
     1441 ctaggaagtt caaactatgg gaagcaatca aacaattgac cccatttaaa ctgaaacgga
     1501 tcaacggtct ggcactaact tgcaaagtac ggatcttatc tactgttcca tattttttct
     1561 ccataacctt cacctcaata ccattatacg aacatacatt ccctttgtaa agaagaaact
     1621 ctgttttatc tttttgactt tttgtctatt acctttctta aaatcatatt ggcacttcct
     1681 gcgaagatga aggacatgta ctaaaaaaaa gaggcagcag tatagattac tgttgcttct
     1741 ttactaattt aggtttactt gttaagaaag attatttacg caaatgttct tcaacaattt
     1801 gctgagattt atcagctgta tcttccactt catcttttgc tttttctaac ttttgaatat
     1861 attgtttcaa gcttgagttt tcagcagttg cttcgtctaa tttcttttgt gtatctgtta
     1921 actgagtttc tacatttgtt ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat
     1981 ttacagtatt ttgtaattct gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat
     2041 ctgcattcag atgattgatt tcatcatttt tttggttgat ctcatctagt ttgacattga
     2101 tttcattttg tttgctttgt agttgtgcat ccttgctgac taattgactt tctaagtttt
     2161 gtttttctgc tgccgattga tttttttggt tttctaattc atttactttt gtttggtatt
     2221 cttggacatc tgcctttgaa tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa
     2281 gatctgtaag cgcttgctct ttatctgtca atgtatgatc aagctgtgtc agattctcat
     2341 tgattgtatc aatatctgtc tctccacccc acaaaataat ttcatttgcg taccgcgtgc
     2401 ctgtgtagcc aattagacat agaaatgcaa cgacaatccc accagtaata aatttctttt
     2461 tcatttgcat agctccttaa tccaatactt ttacatgcta aaagtaagtg atctcaaaca
     2521 caatgtcaag cgtttttaaa ttacatgtgt aattttaata taaatactat caaatactca
     2581 tttaatcctc cttttttatc tatttaaaaa atctttttta tagagaaaaa tgcaggttaa
     2641 tactttctta aataaatatt tatggtgaac aaaaacaaaa aagcaagttt tattaaacgt
     2701 aacccttttc atttcataga ttactgtatt tattacaaaa aacg
//
LOCUS       DAJSBM010000153         2671 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJSBM010000153 DAJSBM010000000
VERSION     DAJSBM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.162"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..321
                     /locus_tag="ONT67_002871"
     CDS             <1..321
                     /locus_tag="ONT67_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356539.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="ONT67_002872"
     CDS             735..1553
                     /locus_tag="ONT67_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCR2356540.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="ONT67_002873"
     CDS             complement(1626..1985)
                     /locus_tag="ONT67_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2356541.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          903 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct tagtctatta tatatctatt tctcttagga gtatgtttag atgtttgaat
     2281 aatagttgcc caactttaaa ggttattctt ataatatttt acttcatttt ccaatagaaa
     2341 attcaaagga ttactggttt ttctaaaata gtttagttta aatacataag tatcctccga
     2401 cactgtatta gttcataatc atctaaatac aaaattttca aataaaaata tttcaagctc
     2461 tatcgttgac gaatgtaaac gatacttata aagtaataga aattgattac atttgtaaac
     2521 ttaaaatggt taatttatct cacataaaac taagaataaa ataatgatta aaagtggtta
     2581 gtttttatac gatttagcct atcggtaaaa atgaattaga gagtgtaaaa tattttgtgt
     2641 aaatgaaaaa atccatacaa aaaaggaagt c
//
LOCUS       DAJSBM010000154         2658 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJSBM010000154 DAJSBM010000000
VERSION     DAJSBM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.163"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(913..1431)
                     /locus_tag="ONT67_002874"
     CDS             complement(913..1431)
                     /locus_tag="ONT67_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCR2356542.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2549..>2658
                     /locus_tag="ONT67_002875"
     CDS             2549..>2658
                     /locus_tag="ONT67_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356543.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          882 a          478 c          393 g          905 t
ORIGIN      
        1 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
       61 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
      121 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
      181 acttatgaaa tgaaagacct tttacatctt gcagaaatcg gtactgggat taaactaagc
      241 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
      301 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
      361 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
      421 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
      481 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
      541 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
      601 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
      661 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
      721 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
      781 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
      841 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
      901 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
      961 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     1021 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     1081 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     1141 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     1201 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     1261 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     1321 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     1381 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     1441 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     1501 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     1561 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     1621 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     1681 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     1741 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     1801 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     1861 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     1921 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     1981 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     2041 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     2101 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     2161 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     2221 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     2281 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     2341 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     2401 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     2461 cccccactta gcttggatag gctacaatta actagacaga aaaattaagg tgtgtagact
     2521 agaagaaaac ataccaggag gaatttttat gtctaagaga acacgaagaa ctttttcaca
     2581 agaattcaag caacaaatcg tcaatcttta cttagctgga aagccacgtg tagaaatcat
     2641 tcgagaatat gaactaac
//
LOCUS       DAJSBM010000155         2543 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJSBM010000155 DAJSBM010000000
VERSION     DAJSBM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.164"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..463
                     /locus_tag="ONT67_002876"
     CDS             <1..463
                     /locus_tag="ONT67_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR2356544.1"
                     /translation="EFHAIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRND
                     IEKFYQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQS
                     AKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            469..1491
                     /locus_tag="ONT67_002877"
     CDS             469..1491
                     /locus_tag="ONT67_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HCR2356545.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1513..>2543
                     /locus_tag="ONT67_002878"
     CDS             1513..>2543
                     /locus_tag="ONT67_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR2356546.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMT"
BASE COUNT         1015 a          268 c          482 g          778 t
ORIGIN      
        1 agaatttcac gctattaaga ataattccgt attattaatt attggaactg gtgatatgga
       61 agaagaaata aaaaattatg caaagaaaag tggcatagat gatgatatta aatttttagg
      121 aaatagaaat gatatagaaa aattttatca agcatttgac gtattaatgt tgccatcatt
      181 atttgaaggg gttccgttag tgggtattga agctcaattt gcagatttac cgtgtttctt
      241 ttcggagaaa gttcctacag aagttgcttt cagtgataag accaatttca taagtttaaa
      301 tcaatcggca aaagaatggg cgagagagat tgcaaatgta gatatctctc atcgagattc
      361 agaacgttcg attttaataa aggcagatta taatattcaa acagcataca aaatcttaga
      421 atcaaaatat tatgagttat ttgaattaat acaaagaggt taaagaatat gacacagaaa
      481 cattcaagta taaaaagaat tatacatcgc atatccgaag aattgaatgc aacaagacga
      541 attgcaaaga taacatcttg gaaggaagca tttgccacgt tacaagctaa aattgatatt
      601 caagtcatga atcgtaatgg atataatgaa agtaaaaaac agaaggaaca tttattaaaa
      661 aaacatgagg taatgctgaa gtattatgaa aagacttttt cagatttttt gaaaacatat
      721 gattatagtc aaaatcaaat aattaatcaa tcaaaatcac catattctaa ctgcatttgg
      781 gtttgttggt ggcagggatt ggatcaatcg ccggaaattg taaaagcttg tgtaaaatca
      841 ataaaacgaa atgcagggaa acatactgtt ataattttaa cagaagataa ttatagacaa
      901 tatgttgata taccaaaatg ggtggaagaa aagaaaaaca aaggaatcat tacgagaaca
      961 aattattctg atttattaag attatctttg ctagcaaaac atggaggttt gtggttagac
     1021 gccacatttt attgtacaaa accaatacca gatgagtatt ttgatatcga tatttggtct
     1081 attaggaggc ctgattatgc tcacgcatcc gtagcatcag gatattttgc gggttattca
     1141 ttagcatgta atcaagagtc tagatggatg tttgctataa ttagagactt ttttttaaat
     1201 tattggaaaa ataatgatat gatggtggat tatttgatga tagattatat gattgtttta
     1261 gcacagagaa taaatcatga aatagcacag acatttgata aaatacctag taacaatcca
     1321 ttatgtgacg aattgattaa aaatttaaat aatatatatg atgaaaaagt atggaactta
     1381 ttaaagaaag atactttact gtttaagttg acatggaaac aatcttttaa aaagcaaaaa
     1441 gatgataaag ttacttttta tgggatgttg gtagaaaaca aattattata aatattagag
     1501 gtataaaaag ttatgaaaca caaggtaagt gtgatagtac caatttacaa tgttgaaaaa
     1561 tatttaagaa aatgtgtgga tagtttattg agtcagacat taaaagagat agaaatcgta
     1621 ttagttgatg atggttcgcc agatgcttcg ggggaaatag cagatgagta tcaaaaaaaa
     1681 tattctaatg taaaaacaat acatagagaa aatggggggt taggaccggc aagaaataca
     1741 ggaattgaga atgcgacagg agaatatgtg gcttttcttg attcagatga ttgggtagaa
     1801 agtgatatgt atgaaaaatt atacttagtt gcgtccaaag aaaatgctga tatagtagtt
     1861 agtggacatt gtgattttgc gaatgacaaa gctatggtga aaaagcaaca tcctttgagt
     1921 gggaatgtat atgatacgcc taatagtatt aaaaatgtac gaaaaaattt atttggacat
     1981 agtccaaaag atcaagaagt agaggccttc ccgatgtcag tatgtatgtc tttgtataat
     2041 aaaaatctgc tagataaata tagactaaga tttgaaaaaa ttttatctga agatacaatt
     2101 tttaacttga atgcatatga tgtcgcaaat aaaattgctt ttacagaata tacagattat
     2161 tgttatagaa aagaagaaca agagtctatt acaaaaagtt tatctccaaa taagcagacg
     2221 gaatttaaag aatttttaat aactctaaaa gctatagcat cgaaagaaga tttagaatgt
     2281 caaatcagag caaaacgtat ggcaattgat tattgtaggc tttatgtggg cttagttgat
     2341 gatgcaaatt tgggaattaa agagaaaaga aagtatataa aaaaatttat tgaagatgag
     2401 ggattaattc aatgttggaa gggatatcct acaaatacgc taccatttca acaaaaattt
     2461 tttcataaaa tgcttgaatg taagtgttat acaatattgt tgttgttaag tagaattaga
     2521 aagataatta aaaatatgac cag
//
LOCUS       DAJSBM010000156         2539 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJSBM010000156 DAJSBM010000000
VERSION     DAJSBM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.165"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(419..598)
                     /locus_tag="ONT67_002879"
     CDS             complement(419..598)
                     /locus_tag="ONT67_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356547.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(595..945)
                     /locus_tag="ONT67_002880"
     CDS             complement(595..945)
                     /locus_tag="ONT67_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356548.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(938..2263)
                     /locus_tag="ONT67_002881"
     CDS             complement(938..2263)
                     /locus_tag="ONT67_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCR2356549.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
BASE COUNT          752 a          506 c          387 g          894 t
ORIGIN      
        1 gtttttttat ttttttatca tgtttaatct tgaaggaatc atattctttt tctaaagaca
       61 ggagcttttc ataaacatca gacatgttta aactagaggc caggcttaaa tcataaacga
      121 atgaagcagg taatgatttt attctacgtt gtcgggttat ccagcttgca accgttcctt
      181 gtgggtaggc atataactca caaaattgtt ctacagtgat tcctaaattc ttgataatgt
      241 aaacattgat cgggtgcgag taaacaaatg tattgataga catggtgcgt aagcctactt
      301 tcttttatta ttgcgtttgc aataatagtt taacacacgc attattctag tatctactcc
      361 tatttgtaaa aacaaaaaag gattcataga gataactcta tagaatccgt aaaatacctt
      421 atatatataa cgggaaaata taaaacctaa aattatgata acctgctgca gcttctacat
      481 agaaagagaa gcgctcaaca ccaacctcat ttatcaaacg ttggccataa ccccaaatct
      541 cttctgatag ctgatcaaag gttatatact ttagctcata ttgctggata atgttcattc
      601 taatcgactc catttattat cctggtaaaa ttcgatatga cgtatttcgt cgtagtgaat
      661 tttgtctttt ccaacgtaga tccccagctc atcactacct aagatcttgc caacaatatc
      721 ttctggatag tatccttcag cgtttcgttc ttctttttga atagatactg atctattttt
      781 tatgactgca gcgttcaata cttccgcaat ctcttcagtc gtcatttgtt cttttgctaa
      841 attttgactg gttcgttgtt cattgtcggc atcaatttct tcagtatgat cagataaata
      901 aaaccctgcc cactttaaac gtccacgatc attgtaatca atcattttta attcccccat
      961 ttccgcccgc atgaccacca acaagtgtgc ttctagtaat gcttctagcg ctttctaaca
     1021 ttgaagaagc atgtacaatc gacgtaaaac catatttttg ccgaatcttg tcaataattt
     1081 tatctaaatt ttcttcgtta atttgctttt gtggatcact aaataaatcc agctgaagcg
     1141 aatcggtgta cacaagcttg ctataggtta ttccaacgtg ccttacttct tgtccttcat
     1201 aatacttatc aaacaagaaa agacaataat tctgcaattc ctttgtatta tctgtgatag
     1261 gtattttcat ctggtgtgaa aatcctggtc tcgtttctaa gatcgacgta ccaatgttta
     1321 aatgaacaca tcctgtttta caattatgct gcctgatacg aatggctact tgttccgcca
     1381 tttcttttac caccagctcg atttcatttc ttctagtata atctcgtggt aacacttgtg
     1441 aattaccata agatttttcc tttgtcggtt ctggcgggat tgctatatct gttcgatcga
     1501 ttccgttagc atggaagtac agctgtaatc caatcacacc taatctattc ttaatcgtat
     1561 aaggattcca gttagcaagg tctctaatgc tcaaaattcc catttggttg agcctttttt
     1621 tcattcgaga accaattccc caaaagtccg tcatttcggg aatattccat actttctcag
     1681 gaacgttttc gtacgtccat tccgcaataa atccttcatt atgctttgct tcattgtcta
     1741 aggctaattt agccaaaagg ggattatcgc cgacgcctac cgtagcaatc aaacctaact
     1801 cttctttaat acgttcttgg atcatttgag cgagcttctt ccttcgctga cttcgtgttt
     1861 cttcagtcgt aaaaagattc agtgattttg tcacttttaa aatcgattca tcgatcgagt
     1921 aaatcagtag atcttcatca gccacatatc ttctgaaaat attatttacc tgcatatttc
     1981 gcttgatata taggttcata cgtggtggaa caacgtgtag tgttttagga aatggttgtg
     2041 gtaagtcacg tggtctactc acatttgtaa taccataccg cttttttgct tcaggagaag
     2101 aagctaatat caatcctgaa ccagtattat cagatcgact cataacaaca agttctgttg
     2161 ttaatggatc taaatttctt tctatacatt cgacacttgc gtaaaagctt ttcatatcga
     2221 ttaaaaagta atcatttact ggctcttttg aataatccat cataaataac actccattct
     2281 tccacattat acaaacatat gttcgatgtt gtaaagaaga aatagctaaa tacataaaaa
     2341 aagaagcata ggtatgctat caccctgctt cttcttatga tttgtatgat caatttattc
     2401 tatcacttta ctaattaatt tgaaaatcta ataaaaactc tcactctaaa agagtgagag
     2461 actgcaaatt agagtacgga cttttttgga gttccgttct ttaatattaa caaatgaata
     2521 aaatccttac aacatttga
//
LOCUS       DAJSBM010000157         2533 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJSBM010000157 DAJSBM010000000
VERSION     DAJSBM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.166"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..379)
                     /locus_tag="ONT67_002882"
     CDS             complement(<1..379)
                     /locus_tag="ONT67_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCR2356550.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="ONT67_002883"
     CDS             598..1968
                     /locus_tag="ONT67_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HCR2356551.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="ONT67_002884"
     CDS             1989..2273
                     /locus_tag="ONT67_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356552.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DAJSBM010000158         2515 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJSBM010000158 DAJSBM010000000
VERSION     DAJSBM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.167"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..1211)
                     /locus_tag="ONT67_002885"
     CDS             complement(<1..1211)
                     /locus_tag="ONT67_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR2356553.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2515)
                     /locus_tag="ONT67_002886"
     CDS             complement(1201..>2515)
                     /locus_tag="ONT67_002886"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCR2356554.1"
                     /translation="EYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDL
                     QSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEF
                     YTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELN
                     TTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADT
                     TFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQ
                     KLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKL
                     SEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVG
                     NDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          699 a          530 c          419 g          867 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgataactt cggtaatcgt
     2341 cacatttcgt tgttgctcat ctgttcctag tttttttgat tggagatcca catcatcaaa
     2401 taacccatta aattgattgt aggtagatgc caattgcaca aaggctttgt ttagatcgtt
     2461 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actct
//
LOCUS       DAJSBM010000159         2454 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJSBM010000159 DAJSBM010000000
VERSION     DAJSBM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.168"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            132..344
                     /locus_tag="ONT67_002887"
     CDS             132..344
                     /locus_tag="ONT67_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356555.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="ONT67_002888"
     CDS             506..1024
                     /locus_tag="ONT67_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR2356556.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="ONT67_002889"
     CDS             1031..1543
                     /locus_tag="ONT67_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356557.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="ONT67_002890"
     CDS             1814..2437
                     /locus_tag="ONT67_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356558.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          408 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaa
//
LOCUS       DAJSBM010000160         2425 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJSBM010000160 DAJSBM010000000
VERSION     DAJSBM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.169"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            121..2274
                     /locus_tag="ONT67_002891"
     CDS             121..2274
                     /locus_tag="ONT67_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCR2356559.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          828 a          425 c          537 g          635 t
ORIGIN      
        1 aaaagttgat tttatcttga aattaggtaa agaatttctt gctccgccca ttttttcttt
       61 tttattaaga agtttttggg tacaatgaag catgaatgta aataggcgga aggataggaa
      121 atgatgaaag attttatgaa aaaaatatta aatacttctt ggttagaaaa aataagaaaa
      181 ggtgcacaag aacctaagaa caaaacgttt cgccggtccc atgttccttt tcgcttaaat
      241 tttctttttt tcattatatt tacgttattt gttgcgttga ttgctagatt gggttatttg
      301 cagatcgtga acggcgaaga gatggaggcg cgagtgaagt ctacttctac gattacggtc
      361 caagaaagta ctcctcgcgg aatgatctac gactccacag gaaaagcttt agttacgaat
      421 aaagcgaatc aggccatcac atatacgaga ggaacacaag ttacggctaa agatttgctt
      481 gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg tagatgaaaa tctgacagat
      541 agagataaaa aagatttttg gctggctgat cccaaacatt tgaaagaagc cacaaaaaga
      601 ctttcggcaa aagaaaagca attagggaac agcgaacaat atagtgcgat ggtcaataaa
      661 gtcacagctg atgagattaa tttaaacgag gagcaattaa aaattgctac catctttaaa
      721 cggatgaata gtgcatatga attaaacact gtctatatca aaaactctgg tgttactgat
      781 caagagttag ctgttgtggc agaacgcgct tctgaacttc caggagtttc gactggcaca
      841 gactggacac gagaatataa tgcagcaagt tcactgaaaa gtattcttgg cagtgtgacg
      901 actgaaaagc aaggacttcc agcagatgaa gcagagaaat acctagcaaa aggttattct
      961 cggaatgacc gtgtaggaca aagttatttg gaaaaacaat atgaagatgt cctgcaagga
     1021 accaaaaccc aatatgaagt ttcccttgat aatgaaggaa atgtttcgaa tcaaaaagaa
     1081 atcttctctg gggaaaaagg ttctaattta atgttatcta tgaatgcaga attccaatca
     1141 aaagtagaag aaattttgaa aaggaattat caaacattga tcaacaacgg caaagcacag
     1201 tattcgcctg gtgcttatgc agtggctatg aatcctcaga caggagaagt gctagcaatg
     1261 actggtttca gtcatgaaca aggttccaaa gaaatcaccg agaatgcttt aggcacgatt
     1321 actagcgcct ttgatccagg gtctgtagtc aaggctggta ctttgacagc aggctgggca
     1381 agtaacgcga tcagcggaaa tcaagttttg attgatgagc cgattcgtct gcaaggtgct
     1441 tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg ctcttgatgc agtcaaagca
     1501 tcggaattgt cttctaatac gtatatgatc aaagtggcgt taaagatgtt aggactagac
     1561 tatacacctg gaatgggact acctagttta gatgaagaag ctaaagctta tcagcaattg
     1621 agagactctt tcaaagagtt tggtcttggt acaacgactg ggatcgattt gccgaatgaa
     1681 tctcctggga tctcacgttc tgttgattat atgaagaagt tcaatgcgga taatggcaaa
     1741 gaatggtatg ctccaggtaa ctttaccgat ttggcatttg gacagtttga cacatataca
     1801 ccaatccaat tagctcaata tgcttcaaca gtcgctaatg gcgggaaacg agtacagccg
     1861 cacctagtca aagcgatcta tggcaatgac gaaaatggaa acttgggtga agtgaagaaa
     1921 gaaattggca caacagtaga aaatacggtc aatatttctg cagaaaatat ggcgatcctc
     1981 cgtgaaggat tccaccaagt tgtccatggt accgatcctt acacaacagc caaaccatta
     2041 gccagtgcga aaatggacct ttccgcaaaa acagggactg cagaaacggt tgcggaaggt
     2101 catccagata ttgcgacagt caatagtaat atcgtggcat acggaccaac agacaatccg
     2161 gaaattgcca tcagtgttgt cttgccgaac ttattagacg aacaagacca cataaacttg
     2221 acgattgcta aagaaataat ggacgcgtat tatgatatgt tcatggcaaa ataaaaaaga
     2281 ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa ttttgtgaaa atagcttctc
     2341 acatttttgc ttaaatttga cttcataccg agaaactggc ttttgaacac cgtttactcg
     2401 gcaaaaaagg ttgtgacaaa aattg
//
LOCUS       DAJSBM010000161         2408 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJSBM010000161 DAJSBM010000000
VERSION     DAJSBM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.170"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(243..464)
                     /locus_tag="ONT67_002892"
     CDS             complement(243..464)
                     /locus_tag="ONT67_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356560.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="ONT67_002893"
     CDS             complement(562..807)
                     /locus_tag="ONT67_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356561.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="ONT67_002894"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="ONT67_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="ONT67_002895"
     CDS             complement(2031..>2408)
                     /locus_tag="ONT67_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2356562.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DAJSBM010000162         2407 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJSBM010000162 DAJSBM010000000
VERSION     DAJSBM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.171"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            5..1150
                     /locus_tag="ONT67_002896"
     CDS             5..1150
                     /locus_tag="ONT67_002896"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR2356563.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="ONT67_002897"
     CDS             1321..1779
                     /locus_tag="ONT67_002897"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR2356564.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="ONT67_002898"
     CDS             1938..>2407
                     /locus_tag="ONT67_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCR2356565.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DAJSBM010000163         2400 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJSBM010000163 DAJSBM010000000
VERSION     DAJSBM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.172"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            207..2069
                     /locus_tag="ONT67_002899"
     CDS             207..2069
                     /locus_tag="ONT67_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2356566.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="ONT67_002900"
     CDS             2069..>2400
                     /locus_tag="ONT67_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2356567.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DAJSBM010000164         2265 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJSBM010000164 DAJSBM010000000
VERSION     DAJSBM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.177"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..958
                     /locus_tag="ONT67_002901"
     CDS             <1..958
                     /locus_tag="ONT67_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR2356568.1"
                     /translation="KCNFRCKHCYNASGGDNCFMDNELTDDELLELTKDICNLKPYNI
                     CFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVSL
                     DGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVNT
                     LKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHI
                     IRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQ
                     DIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            1014..1232
                     /locus_tag="ONT67_002902"
     CDS             1014..1232
                     /locus_tag="ONT67_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356569.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1303..2118
                     /locus_tag="ONT67_002903"
     CDS             1303..2118
                     /locus_tag="ONT67_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356570.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
BASE COUNT          862 a          261 c          395 g          747 t
ORIGIN      
        1 taaatgtaat tttagatgta aacattgtta caatgcgagt ggtggagaca attgttttat
       61 ggataatgag ttgactgatg atgaactgtt agaacttaca aaagatatat gtaatttaaa
      121 gccatataat atttgtttct gtggaggaga aactttactg agaaaaaaaa tagttttatc
      181 ttgtgccact ttattaaaag aggcaggtat tcctaatatt gctattgtta ctaatggatc
      241 attagtaact caggatgttg caaaagaatt attcaaagca ggtattaatc gatttcaagt
      301 aagtttagat ggaaaaaatg ctagtagcca tgaagcactt agattatatc caaacagttt
      361 tgaaaaggca attaatgcta tcaaattatt tagagaattg tccgaagaag tggatgttgc
      421 tttttgtcct actagtttta attacaatca attacgagaa gtatatcaac tagtaaatac
      481 tcttaaagtg aagtctttaa gaattcaacc atttatgctt agtggtaggg gaatgcaaaa
      541 tagggaaata gtgccaactg tttctcaata tagaaaacta gtttctgata ttaactatat
      601 taaaagaaat gaagattata ccaagataga ttggggagat ccaattgatc atatcatccg
      661 acacagaact ttattgaaag atttttcgaa ttttgtttct attaggtcag acggaggatt
      721 aatggcatca ccttacttgc caattcaagt tggaaatatt aaatgtcaca gtctttctaa
      781 atattggcga gcaggacttt ataaagtttt ttctttagat ttagttcaag atatagctac
      841 tcaagtttct tcaatacaag atatgagtaa agaattaaaa aaaggacata ttaaatggct
      901 taatgaagat atagaaattg atattctcga tgaatacaaa catgatattt taatttaggt
      961 ttatcattgg aaagtttaga taaacaaagt acttttagaa aggagggaaa aaaatgattt
     1021 ctgaacctca aatgaataat atgtatgaaa gcccaactat aatgggagct ggtgaagctg
     1081 cgccaagagc ttgttcagca attatggtgt gtgtgttatt agcatctgca ggagtaggta
     1141 tccaagcagg agcggtgtat acttatgctg tagttgctac agtagctgta gctgcagccg
     1201 cagtttggaa aacagtagga ttagttggtt aggaattaat aggagactaa ctgtctccta
     1261 ttgattccaa tgattaagct aaggaggaat tttaagaaaa taatgaaata caataattta
     1321 aaaccgaaat tacttaatga gaacatagaa tatataagag aagaaaatta tggatattta
     1381 accatagttc ccaaaaatgg aactgaattg aaagataacg ttttcaatcc aacagtaaga
     1441 tatatattgg ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa tggtttaaaa
     1501 aagaagtttc ctagtttaga atatgaaaaa ttctatcaag atgttgtagg aactattaaa
     1561 tttttcaaac actataatat tattgatata ggagattata gtatggaaga aaagaaaata
     1621 tataaaattg aacaaaattt agaattagaa ttactaaatg aatcacatta tgaagaaatt
     1681 tataatttta tcacagatca tcctgaaagt atgtattttc atgaagatat ttatcatcaa
     1741 ggaaatagta aagaattaga agttgtcctt agatataatt tattttcgtt tacgagtgaa
     1801 ttttactttt taagacataa agagagaatt gtaggaatgg tttctataat tgttaatcca
     1861 tttcaaaact tatcctacag tatagtaaat tacctcatat gtgagcctgc atatactacg
     1921 actattttga caacagtatt agaaaatatt aacgaagttt caattttaga agtaaagaag
     1981 ctagtgattc atttgctaaa ttcaacagaa gatgaatcac taatccccta tttaaaagat
     2041 tcacattttt ttaaagaagc ggtactatct aaagaatata gagagcatga tgttttgaga
     2101 tacgcttaca tttattaaat aaaatttctg atagtatttt aataattcgg atcatatcat
     2161 aagatatagg agggaaaaga agtgatttta ttgattttgt ctctcgtatt actagttgta
     2221 tctaatctaa tgaaaaataa tcctcctaca aaaattaatt ctttt
//
LOCUS       DAJSBM010000165         2233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJSBM010000165 DAJSBM010000000
VERSION     DAJSBM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.178"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            221..838
                     /locus_tag="ONT67_002904"
     CDS             221..838
                     /locus_tag="ONT67_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356571.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            879..1178
                     /locus_tag="ONT67_002905"
     CDS             879..1178
                     /locus_tag="ONT67_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCR2356572.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1212..1379
                     /locus_tag="ONT67_002906"
     CDS             1212..1379
                     /locus_tag="ONT67_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR2356573.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1424..2029)
                     /locus_tag="ONT67_002907"
     CDS             complement(1424..2029)
                     /locus_tag="ONT67_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356574.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          738 a          362 c          418 g          715 t
ORIGIN      
        1 atggcagacc gttaaggcct gccattttta tgtattttag cggaataaat ataatcatgc
       61 gggagtgtaa acttactgtt tcaaaaagta tactttttga catgccttga ttttctttgt
      121 ttatgtatgg cataataacc acataataca atttgaaacg tttctgattt aatctgattg
      181 ttttgaaaca attttagtga aaaaagggta gataaaaacg atgagaaaat ttttttatat
      241 ccgggtcagt agttatgatc aatcgatcga acgacaactg atttccgcga aggaattagg
      301 aatccctgaa gaatatatat ttgtagaaaa agccagcggc aaagatttta aacgccctga
      361 atatcaatta atgaaacgga tgtttcgaaa aggagattta ctctatctac aatcattaga
      421 tagactaggc agaaataaac aaatgatttt agaagaatgg cgagaattaa ctaaggaaaa
      481 gcagattgat atttctgtgt tagacatgcc tctgttagat actgctagtt acaagcaact
      541 agatgggtta gagacgctta tctctgactt aattctgcaa ttactcagct atatggccga
      601 agacgagcgg aaacgaatta atagccggca aagagaaggc gtagaagctg cgaaacaaaa
      661 gggtgtaaaa ttcggcagaa agaaaattgc tttacctcct gaatttcctg cgatctattc
      721 tgactggaaa gcaggaaaga taactgcagt ctatgctatg gatcaattgg gcatgaaacg
      781 aaataccttt tatcgacggg tgaaagaata tgaacatacc ttgcaccact tttcttgata
      841 gatataacag ctattagacg ggaagggaga gtttgaaaat ggacttaacg aatcaaaggg
      901 tcgtttttac tggaacgctg cagcaaatga cacgaaccca agccatcggt ttggttcact
      961 ctttaaacgg ccattgtcaa tcaagtgtta cttcaaaaac aaatttcctg gtttgtggtc
     1021 agtattctaa gtcgttgttt gatgattctc tctatacaaa aaaggaacaa accgcgcggg
     1081 acttgattgc gttaggtcat gagattacca ttttatcaga agtcgaattt tatgcgttaa
     1141 ttagtcaaca gttgaaagaa tggcaacggt atttataatc gttagtaaaa tttacaagga
     1201 ggaaaatttt tatgaagcga aaatcttatg atcgttgggg cattgaaata actagtttta
     1261 atcgaaatat tgcttttcac gtggagtgtg actgtgggga attagcaaaa agtgtttatc
     1321 aaaaaaatta cttttactgt cctaaatgcc atcgccgata cggggtacat ggaacataga
     1381 aacgaaatag tgcggtagaa aagcgaatga atgtagtata tttctacaaa tcattcgctt
     1441 tctttatttt ataaatagtt ggtcgagaga tcccgttttc ttttgcaatc tctgcaactg
     1501 gaatgccttg ctttaacatg tccaccacgg ttttaaaaac taagcgtttt tgcggatctg
     1561 ttgagttaat agaatactcg attggtcgtc ccttatatcg gccggctttt tgagccaata
     1621 atactccttg ccgctgacgt tcacgaattt tctcacgctc gttttgtgca atataactta
     1681 gcagtgataa aaacatatcg aacatagcgg tatccaggag ttcatttcca gtattaaaat
     1741 ctaaaaactg tgcatctaat atttttaacg ctactttttt ctgtttcata aaagcaactg
     1801 tattttttat atcttcataa tctcttccta aacgatctag tgatgtaacg atgacgcaat
     1861 caccttcacg gatataattt agtaattttt gaaactcagg acgctcagta tctttaccac
     1921 tggctttttc ttggaataat ttatcaatgt taagtccttc catcattgcc aattgtcgat
     1981 ccaaattttg atcttgtgaa gatactcgaa tatagccaat ttttcccata aaaaactcct
     2041 ttttaaaagt gatataattt acatgtaaaa tttacttgta gttttactgc tatcatagca
     2101 tttttaaaaa taaattttat gtagacttaa aatttacttc atggagggtt taaatggatg
     2161 aaatcaaaag taacaaaaaa gcatttggca tttatttagg agaacctagt acagaacaat
     2221 taaaccaatt ttt
//
LOCUS       DAJSBM010000166         2231 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJSBM010000166 DAJSBM010000000
VERSION     DAJSBM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.179"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..543
                     /locus_tag="ONT67_002908"
     CDS             <1..543
                     /locus_tag="ONT67_002908"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2356575.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="ONT67_002909"
     CDS             complement(899..1471)
                     /locus_tag="ONT67_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356576.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="ONT67_002910"
     CDS             complement(1487..2092)
                     /locus_tag="ONT67_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCR2356577.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc t
//
LOCUS       DAJSBM010000167         2124 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJSBM010000167 DAJSBM010000000
VERSION     DAJSBM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.180"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..914)
                     /locus_tag="ONT67_002911"
     CDS             complement(<1..914)
                     /locus_tag="ONT67_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCR2356578.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ONT67_002912"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ONT67_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR2356579.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          719 a          374 c          412 g          619 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaa
//
LOCUS       DAJSBM010000168         2074 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJSBM010000168 DAJSBM010000000
VERSION     DAJSBM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.181"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..511)
                     /locus_tag="ONT67_002913"
     CDS             complement(<1..511)
                     /locus_tag="ONT67_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR2356580.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNI"
     gene            complement(508..1161)
                     /locus_tag="ONT67_002914"
     CDS             complement(508..1161)
                     /locus_tag="ONT67_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR2356581.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1449..>2074
                     /locus_tag="ONT67_002915"
     CDS             1449..>2074
                     /locus_tag="ONT67_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356582.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          667 a          356 c          266 g          785 t
ORIGIN      
        1 gtatattatt ttttattgat tttgcataaa ttaaagaaat aatagcagaa aataaaattg
       61 atattaataa agaatatggt aaaaatttaa ggcttaaatc tttcgcatct ttttgcatat
      121 cagctgtaga aacaaattgt agatttgttt ttttaccatc attaagcttt atttctcttt
      181 cttcaattat caaagaatta ctatcacttt ctaaattaac atttatatta tctttgattt
      241 gtatttcatt cttattattt ttttctttta tgaatacttt tatttcacta ctcttggaat
      301 aaaactccaa agtttgttct atatatttta tttcttttcc attcatatta ctagaaattt
      361 catttgccat attatgaatc ttttcttttc tcgtctccaa atatgtttta ggaaagataa
      421 aaaaaattaa tgaatgaact aatataatta ttattccaag aacagaaaat atttgtatga
      481 acatttttgg aaatatcttt aattttttca ttttctctcc aatttatatc ctacatttct
      541 tatagtagtt atacagtcta attgtaattt ttttctaagt tcttttatat atacatctat
      601 tactcgatca taaggagttt cttcgctatc tttccataca taatcaataa tttgcattct
      661 tgttaacact tgtccattat tatccaataa acattttaat acttctagtt cttttgcatt
      721 tacatctatt tcttcaccat ttattttagc tgtatagcta ttaaaattaa ccgatagatc
      781 cttatattca aacttctcta aatgtccata attcttctta attaaagaat ctattctagc
      841 ttttaagacc ggcaatgaaa atggcttttc tacatatccg tctactaaat tagtaaatgc
      901 atcaatttta tattcttcat cactaaatgc agtcaagatt agaattggta aattgctttt
      961 ctttctaatt tctttcaaca cttctaaacc atttataaaa ggtatctgaa tgtctaagat
     1021 aaccaaattt atatcgctat taaattttga caaggcttct cttccatctt tagcttgaat
     1081 aacagtatat ccaaattctg aaagatattc acttattccc tctcttatca tattatcatc
     1141 ttcaacagtt aatattttca tatatacctc cacttaagcc tatacactag atattataca
     1201 gcatattatg gctctataat atctagtgtt ggtgacacct tctgttgcca atactttttc
     1261 ttttttctaa taaatgcaaa cagacacctt gaaatccttt agaataaagg gttctataat
     1321 attttgtgtt gttggaaaat cgaaggcgat tttctactaa cactttttct tcgtttcaac
     1381 tcaaaaagag actcactttt ggtaaaattt agtcgaccaa aacatcaccc taccaaagga
     1441 gtcgctctat ggataatcat actagaaaat tactcggatt aacagataaa catttatttt
     1501 ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag tttattaaag
     1561 ggagattgga tgactcacct tcccattgtg aacactgtgg atcgattcgt attatccgca
     1621 acggttccta tacgacacgt actcagatat tgaaagtcaa agaaaagttg actattttag
     1681 aattaaaacg tacccgtttt ctatgctatg attgtgggca gaccttttct gcgaaaacgg
     1741 acttagttga tgaacatcat caactaacaa aggaactgaa gcaggcgatt ctcatagaac
     1801 tgtacgaaaa tcaatcacgt aaactaatcg ctaagaagta cttcgtctcg gatgggacgg
     1861 tgacacggat cttgcgtgaa gcaacgaagc attaccagcc acgtatgaac ttccttccga
     1921 cggtgttgtg tatggatgag ttcaaatcaa tgaagtctgt ttctggttcc atgagtttta
     1981 tttgtgttga tgggacaacc catcagctat ttacgatcct tgaagatcgc agactttaca
     2041 aactaactca atacttcctg cgctttccta gaaa
//
LOCUS       DAJSBM010000169         2016 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJSBM010000169 DAJSBM010000000
VERSION     DAJSBM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.182"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..224
                     /locus_tag="ONT67_002916"
     CDS             <1..224
                     /locus_tag="ONT67_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR2356583.1"
                     /translation="RKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELK
                     YFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            225..977
                     /locus_tag="ONT67_002917"
     CDS             225..977
                     /locus_tag="ONT67_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HCR2356584.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            982..1929
                     /locus_tag="ONT67_002918"
     CDS             982..1929
                     /locus_tag="ONT67_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR2356585.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          708 a          268 c          397 g          643 t
ORIGIN      
        1 caagaaaatt tcaattgttt actttagatg aatgtatcaa tgttgactta ttaaataata
       61 aaataagttt tgaagaaaac attgttaaag aaatgcctga atctgctaat gatatgtatg
      121 agaaggagtt gaaatatttc tttgaaaacg tagtaacagg caaagaaaac tggaatgatt
      181 tatatcatgc aaacgaagta ttgaaaatag ctgaggagaa ataaatggaa attttattta
      241 ctatttgtgg tagagctggg tctaaggggt ttaaaaataa aaatttgaaa tatttggcag
      301 gaaagcccct agtccatttc gctttagcaa tgatagatat ctatcaacaa aaacatcctg
      361 aacataattg cacgacttgt ttgaatacgg acagtaatga gttagtagaa ttgacgacta
      421 agttaaatcc gtctgtgaaa attgtagaac gtaaacctga attagctaca gatactgtag
      481 ggaaaattga agtgatacgt gattcattct tacaactaga aagagaagat aatatatttg
      541 atttaattat agatttagat ttgacttcac ctttacgtga agtttctaat cttgaagaat
      601 taatagatga acatctaaaa aatttagata aagacgtcta cttttcagtt gtttctgcaa
      661 gacgtaatcc ctattttaat atggtaaaaa gaattgaaaa tgaagttact atagttaata
      721 aatcaagata cacatctagg caacagacac ctgaagtttt tgaaatgaat gcctcaatgt
      781 attcttttaa acctgaattt ctaaaaaaaa gtaattatat ttttgatggg caatgtggga
      841 taataaaaat gagagattat ctaattttag atattgatag tgaagaagat tttaaatggt
      901 tagaattttt gtttcctaaa tttttagaag attcttcgga gcttcaagat atctataatt
      961 atttatatcg gaggtaaaag gatgagctgt tatcttatca ccggtggggc tggcttcatt
     1021 ggttctacct tagccaatca tttaggagaa gaacatgtgg taatcgtcgt cgatgactta
     1081 tcaatgggaa aagtagaaaa tcttgatatg acgaaaaata tcacttttat tgagggagat
     1141 gtcgctgaga aatccttgat ggaagagatc atgaaagcta ataaatttga ttatatcttt
     1201 catttagcag cagttgctag tgtggcagat tcagttgccc gaccagtaga aacacatcgt
     1261 gtgaattttg aaagtgtatt aatgatttta gaattgatcc gcaagtatca acctgattta
     1321 aaacggatcg tgtttagttc ttcagcggct gtttatgggg atgaaccgac tttaccaaaa
     1381 aaagaagagt ccgtgattcg cccattgacg ccttatgcgg ttgataaatt cgctgcagaa
     1441 caatatgtgc tagattattg tcatttatat gatgtacccg gtagcgcagt tcgttttttc
     1501 aatgtctatg gtccaaatca aaatccgaat tctccataca gtggtgtgat ttctattctc
     1561 gtagatcgct ataagaaaca aatcgtagga gaagcaactt catttacatt atttggtgat
     1621 ggaagccagt ctagagactt tgtttatata gatgatgtaa tccaagcatt acttttagtt
     1681 gcgaatgagg aagctgcact ggggaaacaa ttcaatgttg gaacgggtaa agcaaccaca
     1741 cttttggaat tgattcatgc aatcaatgaa attctcggtg tagaattgac acttgaatac
     1801 aaagaagagc gttcgggtga tatccatgac tctctagcag atattactaa gatccagtca
     1861 attggctatc aaccgaaata tggtatttta agtggaatga ctaattattt gaaaactgaa
     1921 atcaattaaa acatactaag ataagattag agggtaaatt caggctcttt gtcaataagg
     1981 actgatgagt tttgtaaaat caaatctggg atagaa
//
LOCUS       DAJSBM010000170         2002 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJSBM010000170 DAJSBM010000000
VERSION     DAJSBM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.185"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..378
                     /locus_tag="ONT67_002919"
     CDS             <1..378
                     /locus_tag="ONT67_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR2356586.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="ONT67_002920"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="ONT67_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR2356587.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="ONT67_002921"
     CDS             1859..>2002
                     /locus_tag="ONT67_002921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356588.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DAJSBM010000171         1955 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJSBM010000171 DAJSBM010000000
VERSION     DAJSBM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.186"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ONT67_002922"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ONT67_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCR2356589.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ONT67_002923"
     CDS             852..1040
                     /locus_tag="ONT67_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCR2356590.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ONT67_002924"
     CDS             1053..1610
                     /locus_tag="ONT67_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCR2356591.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ONT67_002925"
     CDS             1642..1884
                     /locus_tag="ONT67_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR2356592.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          715 a          305 c          404 g          531 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttc
//
LOCUS       DAJSBM010000172         1796 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJSBM010000172 DAJSBM010000000
VERSION     DAJSBM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.187"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="ONT67_002926"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="ONT67_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCR2356593.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAJSBM010000173         1664 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJSBM010000173 DAJSBM010000000
VERSION     DAJSBM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.189"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            131..505
                     /locus_tag="ONT67_002927"
     CDS             131..505
                     /locus_tag="ONT67_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCR2356594.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ONT67_002928"
     CDS             complement(619..1248)
                     /locus_tag="ONT67_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCR2356595.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ONT67_002929"
     tRNA            complement(1382..1454)
                     /locus_tag="ONT67_002929"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAJSBM010000174         1634 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJSBM010000174 DAJSBM010000000
VERSION     DAJSBM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.190"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            101..868
                     /locus_tag="ONT67_002930"
     CDS             101..868
                     /locus_tag="ONT67_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2356596.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="ONT67_002931"
     CDS             1060..1458
                     /locus_tag="ONT67_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR2356597.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DAJSBM010000175         1611 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJSBM010000175 DAJSBM010000000
VERSION     DAJSBM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.191"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..304
                     /locus_tag="ONT67_002932"
     CDS             <1..304
                     /locus_tag="ONT67_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356598.1"
                     /translation="RFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIE
                     GFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            604..1296
                     /locus_tag="ONT67_002933"
     CDS             604..1296
                     /locus_tag="ONT67_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR2356599.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          685 a          196 c          231 g          499 t
ORIGIN      
        1 aaggtttatg atagataaat ggaaaaagca gtatccacga gtggttaaat tgctcatgaa
       61 tcctgctata ttaacctttt ataatttccc tccgtcaatc agaagaacta tttattcaac
      121 taatttgatt gaaggtttta acaaacaatt aaagaaatat accaaaagaa aagagcaatt
      181 tcctaatgaa gaatctctgg agagatttct agtatctcaa ttcaacaact ataatcaaaa
      241 gtttctatgc cgagttcata aaggttttaa agaaatacat gatacattag aatcaatgtt
      301 ttaacttgat caacagaatg ggcttttcca tttacacata attattgaca caacctattc
      361 tggaaactta caaataaaag cttattcttc agccaccttc gggtggttat tttttataaa
      421 aaaatgtaaa aaaatattga tacataaata aattagttgt agtaaatagt tttagaattg
      481 aatttctcta tcctttttaa ataatttttt agacaaaaaa cagaaagtgt ctaaagaaag
      541 cttgacgaag aaaataattt ttaataactt atttaaggaa attttttgaa ggagaagata
      601 aaagtgagaa aaaagattat tattaatgct ggagtaatta taggattgat gttagtaatt
      661 ttctccggaa agcaatatgc tgataatatt ttattatggg gaggggaaga taatattgaa
      721 aagatcaaca acaatttaac tatattagac acagctctag aaaataaaga gcaaaaaatt
      781 agtattttaa attcacaatt gtcttcaaac aaccaaactc ttgaacaact aagaactaat
      841 attgaagttt ccaaacaaaa agtggcatct ttagaaaacg acaagaatca attagtatca
      901 gagaaaacta atttagaaaa tcaattgtca gcaaaagact ctgaattgca aggaaaacaa
      961 aatgaaatta atgcgaaaat agacgaaatt aaccaaaaag tagctgaaat aaatcgatta
     1021 aatagcgatt ggaataatcg tttacaaaat gaaaagaaca aatttaatga agaacaatct
     1081 actattaata gtttaaacca gcaaattaat caaatgttga gtgaaaaaaa taacattcaa
     1141 aatcaactgc aagctactca gcaaaagcta gaagaagaaa agtcaaaaaa tagtagtttg
     1201 aataattaca ttgaaaagct tcaaaaagct aaaaatgatg tagaggatac cgcaaataaa
     1261 gcacaacaaa ttgtaaatga acatacaatc caatagatat agttttaatt taacaaatgg
     1321 tatgtttgga gataatgaga tagaatataa atgtcataat attatacaat ctaaacaccg
     1381 tttccttttc tatttttgat gacatcagta tatagctact cgaaagagta gtctttttat
     1441 tattctcgct tattattgat atgaaaattt cgatttgaca aaaggtaccg aataattttg
     1501 taatatgaat tattatcaag aagtaattat gatgataaca tatggaaact agttagtaat
     1561 aataacaggt gattaaactt ttgaacgact tgtaattcta tattttttct t
//
LOCUS       DAJSBM010000176         1578 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJSBM010000176 DAJSBM010000000
VERSION     DAJSBM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.192"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            138..752
                     /locus_tag="ONT67_002934"
     CDS             138..752
                     /locus_tag="ONT67_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356600.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="ONT67_002935"
     CDS             complement(776..1126)
                     /locus_tag="ONT67_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR2356601.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="ONT67_002936"
     CDS             complement(1120..1383)
                     /locus_tag="ONT67_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HCR2356602.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          521 a          286 c          245 g          526 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaa
//
LOCUS       DAJSBM010000177         1532 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJSBM010000177 DAJSBM010000000
VERSION     DAJSBM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.193"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          576 a          173 c          312 g          471 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta ca
//
LOCUS       DAJSBM010000178         1520 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJSBM010000178 DAJSBM010000000
VERSION     DAJSBM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.194"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..848
                     /locus_tag="ONT67_002937"
     CDS             <1..848
                     /locus_tag="ONT67_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2356603.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1390..>1520
                     /locus_tag="ONT67_002938"
     CDS             1390..>1520
                     /locus_tag="ONT67_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCR2356604.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVC"
BASE COUNT          502 a          267 c          295 g          456 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaattcggtt
     1321 ctgttgcaaa gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa agacagagag
     1381 gacagagtaa tgaatcattt taaaggcaaa caattcaaaa aagacgtcat tattgtcgct
     1441 gttggttact acctgcgtta caatctaagc tatcgtgaag ttcaggaatt gttatatgat
     1501 cgtggaataa atgtttgtca
//
LOCUS       DAJSBM010000179         1495 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJSBM010000179 DAJSBM010000000
VERSION     DAJSBM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.196"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(522..878)
                     /locus_tag="ONT67_002939"
     CDS             complement(522..878)
                     /locus_tag="ONT67_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR2356605.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT67_002940"
     CDS             complement(892..1302)
                     /gene="spx"
                     /locus_tag="ONT67_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2356606.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          468 a          265 c          213 g          549 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaa
//
LOCUS       DAJSBM010000180         1485 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJSBM010000180 DAJSBM010000000
VERSION     DAJSBM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.197"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            287..694
                     /locus_tag="ONT67_002941"
     CDS             287..694
                     /locus_tag="ONT67_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356607.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ONT67_002942"
     CDS             708..1109
                     /locus_tag="ONT67_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356608.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ONT67_002943"
     CDS             1111..1482
                     /locus_tag="ONT67_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356609.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          570 a          183 c          259 g          473 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatga
//
LOCUS       DAJSBM010000181         1477 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJSBM010000181 DAJSBM010000000
VERSION     DAJSBM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.198"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            191..445
                     /locus_tag="ONT67_002944"
     CDS             191..445
                     /locus_tag="ONT67_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR2356610.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="ONT67_002945"
     CDS             446..763
                     /locus_tag="ONT67_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2356611.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          527 a          216 c          282 g          452 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatat
//
LOCUS       DAJSBM010000182         1453 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJSBM010000182 DAJSBM010000000
VERSION     DAJSBM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.199"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..625)
                     /locus_tag="ONT67_002946"
     CDS             complement(<1..625)
                     /locus_tag="ONT67_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HCR2356612.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="ONT67_002947"
     CDS             complement(784..1050)
                     /locus_tag="ONT67_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR2356613.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="ONT67_002948"
     CDS             complement(1040..1396)
                     /locus_tag="ONT67_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR2356614.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DAJSBM010000183         1437 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJSBM010000183 DAJSBM010000000
VERSION     DAJSBM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.200"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            105..1406
                     /locus_tag="ONT67_002949"
     CDS             105..1406
                     /locus_tag="ONT67_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2356615.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          288 c          264 g          412 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttct
//
LOCUS       DAJSBM010000184         1417 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJSBM010000184 DAJSBM010000000
VERSION     DAJSBM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.201"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(150..1337)
                     /locus_tag="ONT67_002950"
     CDS             complement(150..1337)
                     /locus_tag="ONT67_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCR2356616.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAJSBM010000185         1320 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJSBM010000185 DAJSBM010000000
VERSION     DAJSBM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.204"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(31..>1320)
                     /locus_tag="ONT67_002951"
                     /note="aph(2'')-Ia"
     CDS             complement(31..>1320)
                     /locus_tag="ONT67_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCR2356617.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          436 a          226 c          100 g          558 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
      481 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
      541 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
      601 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
      661 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
      721 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttattagt
      781 actaaatttt gttttaaaaa tgtattcatt attaactaaa tatgccacac tatcataacc
      841 actaccgatt atttcaatac tatctacttt gaaattatca aagtaatgct caattaaata
      901 tttcattgcc ttaacatttg tggcattatc atcatatcta tattccatta aataacaatc
      961 ttcttttttg ccctcgtgta attcatgttc tggcaaatct tcaataattc taaaaccaga
     1021 tttttggtat gcccttattg ctcttggatt atttttatga gggtctaaaa taactgcatt
     1081 agcatttctt tcttttttca aaaattcaaa aatcaattta atatatcttg taccaattcc
     1141 tttactccaa taatttggct ctcctataaa ttgatccata ccatagacta tctcatcagt
     1201 ttttggataa tgataatcag tatataactc atcatacatt ttatatattt gtccatatcc
     1261 aataggaaca ttgttatatt caataattac tctaaaaact tcatcttccc aaggctctgt
//
LOCUS       DAJSBM010000186         1309 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJSBM010000186 DAJSBM010000000
VERSION     DAJSBM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.205"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..229
                     /locus_tag="ONT67_002952"
     CDS             <1..229
                     /locus_tag="ONT67_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356618.1"
                     /translation="YLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRN
                     GYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            239..442
                     /locus_tag="ONT67_002953"
     CDS             239..442
                     /locus_tag="ONT67_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356619.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            653..1237
                     /locus_tag="ONT67_002954"
     CDS             653..1237
                     /locus_tag="ONT67_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR2356620.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          461 a          188 c          250 g          410 t
ORIGIN      
        1 ttatttaata gtttctccat tacttgctct agcagcttct aaaacgatac gagtgacagg
       61 aacgatgatt tttgataaag ttggttggcc ttctagacct cctcaatcac ggaattggaa
      121 agaaaaaaga aatggttatt ggtactatgg aaaattgtat ttacaaagtt ataaatcagc
      181 tggaaacaat tggattgcga cttattcagg aaccttaact agaaagtagg gattttttat
      241 gccagaattt gtgaacacac caaattttta tcaagatagc gatattctaa gtaaaattaa
      301 tttaccgaaa gaagaatcag ctttacaagt tcagcgaact ttacaggaga cacaaaaaat
      361 tcaaaaatca atttctaatc tagaagattc tgaagaaaaa agaaaaaaaa gagaccaaaa
      421 aacatatcaa aaagaaaaat aatgatttta aaaaaagagt gtctagtcga cattcttttt
      481 ttctaattga aagtgttcat aaaatcaccc atttctggac acttcctttt tttgctttat
      541 ttttcaaaaa aacaattcat ttcatgttca taaacccata acaatatcta tgttcagtta
      601 aactatatgg ataaagttat gttacaataa aattaatgaa ggagtgattt ttttgaaaat
      661 aggatacgcc cgggtatcta ctggtttaca aaatttggat ttacaagaag acagcctaaa
      721 taaatttggt tgtgaaaaaa tttttactga tcatatgagt ggggcaaaaa gtaatcgacc
      781 aggattagaa atggccattg attttgttcg ctctggcgat acacttgtcg tttggcgctt
      841 agatcgttta ggtagaaata tggaagattt gatttcaatc gtcaatcgac tgaatgagcg
      901 tggtgtgagt tttcatagta ttcaagaaaa tattacgatg gacaaatcaa gttctacagg
      961 acagctcatg tttcatttgt ttgcggcatt tgcagaattt gagcgtaatt tgatcctgga
     1021 acgatccgca gcaggacgag cagctgctcg tgcaagagga cggtttggtg gtcgtcctga
     1081 aaaattgtct gaacaagatt tggaattact gaagacttta gtggatagcg gtacacctat
     1141 taagactatt gcggaacggt ggaacgtctc aagaaccacc atttatcgtt atttggcaaa
     1201 aatggaggag aaaaatggaa agacaaattc tcattagttt ttgtcattta atgctgaaaa
     1261 tttttgccat ttatctagaa aagtgacaat agtatgagaa ttttttgta
//
LOCUS       DAJSBM010000187         1270 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJSBM010000187 DAJSBM010000000
VERSION     DAJSBM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.206"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(61..279)
                     /locus_tag="ONT67_002955"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="ONT67_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="ONT67_002956"
     CDS             complement(545..739)
                     /locus_tag="ONT67_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356621.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="ONT67_002957"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="ONT67_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          448 a          214 c          174 g          434 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt
//
LOCUS       DAJSBM010000188         1255 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJSBM010000188 DAJSBM010000000
VERSION     DAJSBM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.207"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            14..1093
                     /locus_tag="ONT67_002958"
     CDS             14..1093
                     /locus_tag="ONT67_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2356622.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          330 a          341 c          355 g          229 t
ORIGIN      
        1 aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg gaaaacacct
       61 aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt ccaaccgtca
      121 ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca aacgcgggac
      181 cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt atcatgacgt
      241 ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact gcggcagacg
      301 gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga tgctgttgga
      361 aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt tcgacccggc
      421 cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca tgaaaacgaa
      481 gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc atggcaaccg
      541 cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg ttggtacccg
      601 gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca agtcggatgc
      661 cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga agctggccgg
      721 gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg ctggtgcgta
      781 cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg cgggactgca
      841 cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt cctgggagcg
      901 cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg gcagcccaat
      961 cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg attacccgcg
     1021 aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta ggcaggaacg
     1081 gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg cttgtgtctg
     1141 gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat tgtcaagggc
     1201 ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca ccatg
//
LOCUS       DAJSBM010000189         1246 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJSBM010000189 DAJSBM010000000
VERSION     DAJSBM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.208"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            200..742
                     /locus_tag="ONT67_002959"
     CDS             200..742
                     /locus_tag="ONT67_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356623.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ONT67_002960"
     CDS             898..>1246
                     /locus_tag="ONT67_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HCR2356624.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAJSBM010000190         1233 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJSBM010000190 DAJSBM010000000
VERSION     DAJSBM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.209"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            358..444
                     /locus_tag="ONT67_002961"
     CDS             358..444
                     /locus_tag="ONT67_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCR2356625.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(565..>1233)
                     /locus_tag="ONT67_002962"
     CDS             complement(565..>1233)
                     /locus_tag="ONT67_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356626.1"
                     /translation="LLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLS
                     IVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMND
                     IFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNL
                     FSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQ
                     QQQN"
BASE COUNT          348 a          244 c          205 g          436 t
ORIGIN      
        1 taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc
       61 aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg
      121 ctaaaaacat gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt
      181 aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc
      241 aagccctaac aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta
      301 agatataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg
      361 tttgaattgt tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg
      421 ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc
      481 cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta
      541 gtttaacatg attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac
      601 actttttaga taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg
      661 ctgaagcagt gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg
      721 ttcactagtt gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg
      781 cgtatcttta aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt
      841 ttgctgtttt tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt
      901 caaacgtttt tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc
      961 ttccattttt tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt
     1021 ttgagtgata tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc
     1081 gaataaacca cttaaaacga ttgataatgt aaatataacc cctggcaata aaaacctctt
     1141 aaatcgaatt ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca
     1201 aataattcct aaaatactgg caaaaatgag tag
//
LOCUS       DAJSBM010000191         1218 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJSBM010000191 DAJSBM010000000
VERSION     DAJSBM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.210"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..383
                     /locus_tag="ONT67_002963"
     CDS             <1..383
                     /locus_tag="ONT67_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2356627.1"
                     /translation="FSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKG
                     CPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVG
                     YRNQRLAQRILDNELGCANASEAV"
     gene            complement(367..960)
                     /locus_tag="ONT67_002964"
                     /pseudo
     CDS             complement(367..960)
                     /locus_tag="ONT67_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          371 a          241 c          212 g          394 t
ORIGIN      
        1 catttagccg tatcaaacaa cctctgacag aggttgagat ttttcatact gatcgtggaa
       61 aagagtttga taaccaagct attgatgaat tattaacaac ttttgacatc aatcgatcat
      121 tgagtcataa aggctgtcct tttgataatg ccgtagctga atcaacttat aagtcgttga
      181 aagtagaatt tgtctatcaa tacacatttg aaaccttaca acaattggat ttggagttat
      241 ttgactatgt aaattggtgg aaccaccttc ggttgcacgg tacacttggc tacgagacac
      301 cggttggtta ccgtaaccag agattggcgc agcgaatcct tgataatgag ctcggatgtg
      361 ctaacgctag cgaggcagtc taactttaac tgttagctcc tgccgaagat cgtcacatcc
      421 gaggaggctc attgtcaagg acaatcggaa tagcatacgg aaagaagttc tgcaccttat
      481 aaaatttgtc aaaaaaactg ttgccattcc accagacact tgcttaaagc gaaaaatttc
      541 ttcaatgtcc atgcctaatt ctttggatat tctcttgtct gaccatccat tttttactaa
      601 tctttggatg acaatttcca ttcctcttat ttcatgggtc cctcttgcac gattgtgtcg
      661 aattgttgaa gccatacgtt cttatatggg tttatcaata actgttaccg gtacaacttt
      721 taagttgagt ctttccttag caacacgata cctatgaagc acatcaacca agatatactt
      781 ctcttctttt tccttgtaat aacaaactaa tggttgagta aagccatctt caatgataga
      841 aagctccaat aagttcattt ccggagacgc tactcgattg ggattataat cgttcgcctg
      901 tattttggta attggaacaa gccgaacgtc catcacaggg aaagttatgt ctttattcat
      961 acgcttgtat accctacttt cttaaaaagt agggtatact caagaatccg attaattatc
     1021 ttctcaattt ggataatata tctcatcagt cttcttgtca ctttatcaac atgattttgc
     1081 tgcaaaatgg gataccgaca gtactggact ggtaatcagg ataaaagcaa aaataattaa
     1141 tttgtatcct aaaaaatcca taaaaattct ctcacatttt cgggtattaa ttcgagaatc
     1201 acacctataa atttatta
//
LOCUS       DAJSBM010000192         1214 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJSBM010000192 DAJSBM010000000
VERSION     DAJSBM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.211"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            70..1026
                     /locus_tag="ONT67_002965"
     CDS             70..1026
                     /locus_tag="ONT67_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356628.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="ONT67_002966"
     CDS             complement(1080..>1214)
                     /locus_tag="ONT67_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2356629.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DAJSBM010000193         1199 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJSBM010000193 DAJSBM010000000
VERSION     DAJSBM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.212"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..867
                     /locus_tag="ONT67_002967"
     CDS             <1..867
                     /locus_tag="ONT67_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR2356630.1"
                     /translation="VGDKKVQFTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFG
                     RRSADGLQRERFVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNEL
                     ESSYSKTMFRLLKQYRTKGIAYFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAI
                     FKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYS
                     EKDPTSLQEKIKEQKRKLELLQQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKK
                     GLLDNLQNLFKE"
     gene            877..>1199
                     /locus_tag="ONT67_002968"
     CDS             877..>1199
                     /locus_tag="ONT67_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356631.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLD"
BASE COUNT          528 a          185 c          177 g          309 t
ORIGIN      
        1 gtaggcgata aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa
       61 gcaactgcaa atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat
      121 ttacgttttg gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat
      181 caatttaaaa tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa
      241 gccttaccct tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat
      301 gaattagaaa gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa
      361 ggaattgcct atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat
      421 agagaaagtg atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc
      481 ttcaaaggtc tgacaatcaa aaagaaatac ggaaaaggaa gaggaaaacc tgtcattggc
      541 tatcaattca cgtttaaacc tgaaataaaa aaagcagacg actttaataa acacgcacca
      601 acaaaagaag aacaaaataa agctttagaa ctagttaatc aacaaattta ctctgaaaaa
      661 gaccccactt cattacaaga aaaaatcaaa gaacagaaac gaaaattaga acttttacaa
      721 caattagaag agctagaaag aaaacaaaat aatctgaata aagaaaataa ggagattaac
      781 gaacaaatac cagataacaa ttccgatatt cccccagaat taaaaaaagg cttactagat
      841 aatcttcaaa acttatttaa agaataaggg gtttttatga cagataaaac tatcaaagaa
      901 ttagcagata ttctaggtgt ttcaaaagat aaagtgaagt atcaactgag gaaattacct
      961 agtaatttta cctacaaaaa aggaaatatt atctacctga aaaaagaagc agtaacaaca
     1021 attacaaagg ttttatcagg taatgagccg ggtaacttac ctagtgaatt acctgattca
     1081 ttacctaccg attacctact caaggagatt gaaactaaga ataatcatat tgaagaaaaa
     1141 gataggcaaa ttgaaaaact tcaactagct ttagaaacac aacaaaaatt actcgacca
//
LOCUS       DAJSBM010000194         1193 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJSBM010000194 DAJSBM010000000
VERSION     DAJSBM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.213"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1076
                     /locus_tag="ONT67_002969"
     CDS             <1..1076
                     /locus_tag="ONT67_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2356632.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAJSBM010000195         1176 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJSBM010000195 DAJSBM010000000
VERSION     DAJSBM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.214"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>1176
                     /locus_tag="ONT67_002970"
     CDS             <1..>1176
                     /locus_tag="ONT67_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR2356633.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVL"
BASE COUNT          385 a          208 c          257 g          326 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tacttt
//
LOCUS       DAJSBM010000196         1148 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJSBM010000196 DAJSBM010000000
VERSION     DAJSBM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.215"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..830)
                     /locus_tag="ONT67_002971"
     CDS             complement(<1..830)
                     /locus_tag="ONT67_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HCR2356634.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..>1148)
                     /locus_tag="ONT67_002972"
     CDS             complement(901..>1148)
                     /locus_tag="ONT67_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCR2356635.1"
                     /translation="KNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERNYSN
                     YKKIFVFLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
BASE COUNT          367 a          206 c          162 g          413 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttat
//
LOCUS       DAJSBM010000197         1143 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJSBM010000197 DAJSBM010000000
VERSION     DAJSBM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.216"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT67_002973"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="ONT67_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR2356636.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DAJSBM010000198         1083 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJSBM010000198 DAJSBM010000000
VERSION     DAJSBM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.217"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>1083
                     /locus_tag="ONT67_002974"
     CDS             82..>1083
                     /locus_tag="ONT67_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HCR2356637.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     "
BASE COUNT          364 a          197 c          212 g          310 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tat
//
LOCUS       DAJSBM010000199         1066 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJSBM010000199 DAJSBM010000000
VERSION     DAJSBM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.218"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            434..1066
                     /locus_tag="ONT67_002975"
                     /pseudo
     CDS             434..1066
                     /locus_tag="ONT67_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          369 a          186 c          188 g          323 t
ORIGIN      
        1 ctcactatat aaaatgtgat taattttatt attaataaaa tattttttat attacaaaag
       61 tagtgtttca aagttagtag agaaaatgta gttatatatt taaattttta ccagaaagaa
      121 atgaacacct tcaattatct aaatctcgaa aatggttaga tgtaatcatc aggaaaaaca
      181 atcttaaacg aattactatt catggtctta gacacacaca tgctagcttg cttttagcaa
      241 gctagtgcaa atattaaaga tgtgcaagag cgtttaggcc actcttctat tcaaatcatt
      301 atggatctat atattcatat tacagacaaa agaaaagaag aaaccgtagc gcaattcgca
      361 aaatataggc tctataattt atgggactga tgaatcaaaa ttgattcttc agtcccattg
      421 ttattttagg tatatgattc gtacattgaa agagttgaaa aactatcttc cgaagccgtc
      481 aaaaaggacc ctgccaaaag tattgatggt ggatgaattt cgttcgcacg cttcaattgt
      541 ggataaaatg agctttattt gtgcggatgg cgagactgga aaactcattg atattttgcc
      601 tacacgaaaa ttgtctcgat taacacacta ttttagtcgc tgtgcaaatc ctgaagcggt
      661 ggaatattta gtgacagata tgaacgccgc ctacttccaa ctgacaaaac gtgtcttacc
      721 aaatgcgaaa gtcgtcattg attgttttca tatcatcaaa catttgaatc aggcgttcaa
      781 tgattttcgt gtacaagaaa tgaaaagatt acaccagtta gggcaacgac gggaagccga
      841 gaaagtgaaa aagaactggc gctttcttct aaaaaaccgt caaaaaatca atcactatga
      901 ttacaaaaca tggcgcagtt ttcgtgcacc taaatacccg ctactcactg aagcaatgat
      961 gattgatcga ttgctggcat tttcaacgcc tttaaaggac gcctatactt ttttccatga
     1021 gctgaccgaa gcctttagag acaaggaatc agacttattt tttacc
//
LOCUS       DAJSBM010000200         1051 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJSBM010000200 DAJSBM010000000
VERSION     DAJSBM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.219"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="ONT67_002976"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="ONT67_002976"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCR2356638.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DAJSBM010000201          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJSBM010000201 DAJSBM010000000
VERSION     DAJSBM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.220"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(25..912)
                     /locus_tag="ONT67_002977"
     CDS             complement(25..912)
                     /locus_tag="ONT67_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCR2356639.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DAJSBM010000202          994 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJSBM010000202 DAJSBM010000000
VERSION     DAJSBM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.221"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>994)
                     /locus_tag="ONT67_002978"
     rRNA            complement(<1..>994)
                     /locus_tag="ONT67_002978"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DAJSBM010000203          986 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJSBM010000203 DAJSBM010000000
VERSION     DAJSBM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.222"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..704
                     /locus_tag="ONT67_002979"
     CDS             <1..704
                     /locus_tag="ONT67_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2356640.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DAJSBM010000204          980 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJSBM010000204 DAJSBM010000000
VERSION     DAJSBM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.223"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            82..>980
                     /locus_tag="ONT67_002980"
     CDS             82..>980
                     /locus_tag="ONT67_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCR2356641.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYE"
BASE COUNT          334 a          182 c          192 g          272 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata
//
LOCUS       DAJSBM010000205          931 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJSBM010000205 DAJSBM010000000
VERSION     DAJSBM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.224"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>931
                     /locus_tag="ONT67_002981"
     CDS             <1..>931
                     /locus_tag="ONT67_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCR2356642.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DAJSBM010000206          902 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJSBM010000206 DAJSBM010000000
VERSION     DAJSBM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.225"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>902)
                     /locus_tag="ONT67_002982"
     rRNA            complement(<1..>902)
                     /locus_tag="ONT67_002982"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          188 a          274 c          185 g          255 t
ORIGIN      
        1 tacctatcct gtacatgcgg tacagacact caatatcaaa ctacagtaaa gctccatggg
       61 gtctttccgt cctgtcgcgg gtaacctgca tcttcacagg tactaaaatt tcaccgagtc
      121 tctcgttgag acagtgccca aatcgttacg cctttcgtgc gggtcggaac ttacccgaca
      181 aggaatttcg ctaccttagg accgttatag ttacggccgc cgtttactgg ggcttcaatt
      241 cgtaccttcg cttgcgctaa gcactcctct taaccttcca gcaccgggca ggcgtcagcc
      301 cctatacttc atcttacgat tttgcagaga cctgtgtttt tgataaacag tcgcttgggc
      361 ctattcactg cggctgatcg tatgatcagc accccttctc ccgaagttac ggggtcattt
      421 tgccgagttc cttaacgaga gttctctcgc tcaccttagg attctctcct cgactacctg
      481 tgtcggtttg cggtacgggc cgctgttttc tcactagaag cttttctcgg cagtgtgaca
      541 tcaggaactt cggtactatt atttccctcc ccatcacagc ttgtccttaa gaaaagaagc
      601 atttgactcc tcttcagact tactgcttgg acagacattt ccgatcgtct gcattcctta
      661 gcctcctgcg tccctccatt gctcaaacaa aaacaacggg tacaggaata tcaacctgtt
      721 atccatcgcc tacgcctgtc ggcctcggct taggtcccga ctaaccctgg gcggacgagc
      781 cttccccagg aaaccttagt cattcggtgg acaggattct cacctgtctt tcgctactca
      841 taccggcatt ctcacttcta agcgctccag cagtcctcac gatctgcctt caacgccctt
      901 ag
//
LOCUS       DAJSBM010000207          890 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJSBM010000207 DAJSBM010000000
VERSION     DAJSBM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.226"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..888
                     /locus_tag="ONT67_002983"
     CDS             <1..888
                     /locus_tag="ONT67_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCR2356643.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAJSBM010000208          890 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJSBM010000208 DAJSBM010000000
VERSION     DAJSBM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.227"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            6..>890
                     /locus_tag="ONT67_002984"
     rRNA            6..>890
                     /locus_tag="ONT67_002984"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          271 a          174 c          265 g          180 t
ORIGIN      
        1 gataaggtta agtgaataag ggcgcacggt ggatgccttg gcactaggag ccgatgaagg
       61 acgggactaa caccgatatg ctttggggag ctgtacgtaa gctatgatcc agagatttcc
      121 gaatggggga acccaacatc tttaatagga tgttacgatt gtgtgaatac atagcacatt
      181 cgaggtagac gcagagaact gaaacatcta agtacctgca ggaagagaaa gaaaattcga
      241 ttccctgagt agcggcgagc gaaacgggaa aagcccaaac cagcaagctt gcttgttggg
      301 gttgtaggac tccaatatgg tagttctttc agatagtcga atgacttgga aaagtcagtc
      361 aaagagggta aaaaccccgt agacgaaatg tggaagacac ctaggaggat cctgagtacg
      421 gcggaacacg agaaattccg tcggaatccg ggaggaccat ctcccaaggc taaatactcc
      481 ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc cggaagggga
      541 gtgaaataga acctgaaacc gtgtgcctac aacaagtcaa agcccgttaa tgggtgatgg
      601 cgtgcctttt gtagaatgaa ccggcgagtt acgattgcat gcgaggttaa gttgaagaga
      661 cggagccgca gcgaaagcga gtctgaatag ggcgtttgag tatgtagtcg tagacccgaa
      721 accatgtgat ctacccatgt ccaggttgaa ggtgcggtaa aacgcactgg aggaccgaac
      781 ccacgtacgt tgaaaagtgc ggggatgagg tgtgggtagc ggagaaattc caaacgaact
      841 tggagatagc tggttctctc cgaaatagct ttagggctag cctcggaatt
//
LOCUS       DAJSBM010000209          849 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJSBM010000209 DAJSBM010000000
VERSION     DAJSBM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.228"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            76..>849
                     /locus_tag="ONT67_002985"
     CDS             76..>849
                     /locus_tag="ONT67_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356644.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNFHLDSTHIKANA
                     NKHNFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEQEKQFAYSAHTSCDDNGFILSTIITPGNIHDS"
BASE COUNT          280 a          158 c          165 g          246 t
ORIGIN      
        1 gccagactgt agacaaagtc cattttatgg atgatgtcta cagtcttttc ttttataata
       61 gatttatgaa aacgaatgca aggagtgata gctatgatgt ctaaaaacaa tacgaatggt
      121 agaaatcaat ttgcgatgct tacgattgat gatctagttc ctcaagatca tctcgttcga
      181 aagattgatg ccgcgcttga ttttgaattt atttatccaa tcgtcgaagc cacttactcc
      241 gatttaggtc gcccaagtat cgacccagtg attctgatca aattggtgtt tattcagtat
      301 ttattcggta tccgttccat gcgacaaacc attaaggaag tcgataccaa tgtcgcttac
      361 cgctggtttt taggctactc cttcgaagag aaaatccccc atttctcaac ttttggaaag
      421 aattatgttc gccgatttag agaaacgacg gtgtttgagg atatttttgc ctatatttta
      481 gaacaagcag ttaaagcagg ttttgtaaca gaggataatt ttcatctaga ttctacccac
      541 atcaaagcta acgccaataa acataatttt acgaaagaaa tgacccatga tgaagcgaaa
      601 gcctatcagg atgaattaga agatgagatc aatcgtcaac gaatagaggc tggaaaacgc
      661 ccttttactt tggacttaga aaaggaagtg aagctgaaag aaagaaaaat aagtaaagct
      721 gatccggaaa gcggctacta cgtcaaaggc gaacaagaaa aacaatttgc ttattctgcc
      781 catacttcat gcgacgacaa tggatttatt cttagcacaa tcatcactcc tggaaatatt
      841 catgacagt
//
LOCUS       DAJSBM010000210          815 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJSBM010000210 DAJSBM010000000
VERSION     DAJSBM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.229"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            533..>815
                     /locus_tag="ONT67_002986"
     CDS             533..>815
                     /locus_tag="ONT67_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR2356645.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
BASE COUNT          270 a          130 c          172 g          243 t
ORIGIN      
        1 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcgtaa attagagcgt
       61 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
      121 tgccgaggat aaggagttga cggctaaacc actaatccct gaagctgaca tttgctaaaa
      181 aatggcaagt ccattttttg gccaacctag cgttagcgaa gtttgggggc gcccttttca
      241 aagggtgcgg ttttattttt tggatatttg catagctaaa gagaagtgct attattgagt
      301 tatcaaataa ataaactgaa aaaagagcaa aaaaataaag tcgagcaacg ctggacacgt
      361 tctcgacctt tacggaaact aatcgcacta gctccgtaac ttcttaatca ttatacgaat
      421 atttttagat ttcttcaaga ctagagattt tttggtaatg aatttttaag agttacgtga
      481 acttttgaaa attctttcca gaagtctctt tttctttgag gaggaacaaa atttgagtaa
      541 aaaaggcacg atcataaatg agtgggaaaa attaacagcc ccctctaaca ggcggttaaa
      601 gtatcaaagt ttgacccttc ccttaaaaat gcagtggtca attgaccgaa ttaccatagt
      661 cggaaaactg aaagaaaata tttattatca cactccaaat gatgtgttga ttttaaattt
      721 tgaacagctt atgcgcctaa atgaaggtaa tggttattta agatctgtta gtaataatgg
      781 ttggcagttg ttagaccaac atgaagagaa tattg
//
LOCUS       DAJSBM010000211          811 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJSBM010000211 DAJSBM010000000
VERSION     DAJSBM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.230"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..672
                     /locus_tag="ONT67_002987"
     CDS             <1..672
                     /locus_tag="ONT67_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356646.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAJSBM010000212          779 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJSBM010000212 DAJSBM010000000
VERSION     DAJSBM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.231"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            245..643
                     /gene="spxA"
                     /locus_tag="ONT67_002988"
     CDS             245..643
                     /gene="spxA"
                     /locus_tag="ONT67_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCR2356647.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          274 a          111 c          148 g          246 t
ORIGIN      
        1 acgctgtttc atatgtttag aaaatttgaa ttagatttga aaactctaat ttttaaatga
       61 taaaaaaagc gagaatgctg gttttactgt atattaacct tagataactt tcattttttt
      121 aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat tatgtataat agaaacagat
      181 gggagattat tatgatcttc tgttacaaaa aagtgattct tatcacgaaa aggagtggtg
      241 cacgttgtta acattatata cttccccaag ttgtacttcc tgcagaaagg ctcgtgcgtg
      301 gttgcaagaa catcagattc catttgttga acgtaatatt ttttcagaac cattgaacag
      361 ttctgaatta aaagcgattt tacaaatgac agaagacgga acagaagaaa tcatctcgac
      421 acgttccaaa gtattccaaa aactgaacat ggatttagat gatctgccgt tacaagaatt
      481 attggaactt gtgcaaaaca atcctggatt gcttcgccgt ccaatcatga ttgatgataa
      541 acgtctgcaa gttggtttta atgaagatga aattcgtcgg ttcctaccgc gcgatgtacg
      601 acaattagaa ttacgtcaag cacaattgat ggctggatta tagaaggata atacgaaact
      661 tggaaaagac tggctctttg tcaaatgatg ttggtaaaga gaaatcttag aataatttgg
      721 acagaacaga aagaagaaaa cctctttctg ttctgtttat ttggttaaaa atgttttgc
//
LOCUS       DAJSBM010000213          764 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJSBM010000213 DAJSBM010000000
VERSION     DAJSBM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.232"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(109..>764)
                     /locus_tag="ONT67_002989"
     CDS             complement(109..>764)
                     /locus_tag="ONT67_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HCR2356648.1"
                     /translation="GRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPL
                     EAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDV
                     KLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          213 a          175 c          146 g          230 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccga
//
LOCUS       DAJSBM010000214          757 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJSBM010000214 DAJSBM010000000
VERSION     DAJSBM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.233"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(133..>757)
                     /locus_tag="ONT67_002990"
     CDS             complement(133..>757)
                     /locus_tag="ONT67_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR2356649.1"
                     /translation="FLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFV
                     SNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIV
                     GTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNN
                     YSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          252 a          136 c           81 g          288 t
ORIGIN      
        1 caaactcttt ttttccactg ctactataag ctgttatatc aaaattaata gaattctttt
       61 ttccatagct tattagattt tttagatgtc tctttccaat tgaccctaac ccaaaaaaaa
      121 ttactttcat cattaattta cctcaatgtc gttaattaag ttaataatat acttatcttt
      181 ttcaaaagta tattggggtt ccattttctt ttccaaaagc gtgaaaaatt ctttaatctc
      241 ctctaaatat gcatcttcaa taatagtatg tgaataatta ttatcatgca taacctcatc
      301 gtacaattga atatctgtca tactagaagt ttcttcattc catgaaaata gagaatccgg
      361 gttaccttcc cattctatta aaatcttttc accaattata gttaaatttc ttttagctac
      421 tcttgaaaca acatctacac ttaaagttcc aactatacca gtttcatgct caataataag
      481 agtgaaactg tccgaataat caatatcaag attggataat ttctgggtca ttacagtaaa
      541 cgatttaact ttcccaaatg tatctataat ccaaggtaat tcaatagcga atatttctct
      601 acacccatta gtttttttgt ttgaaacaaa aaaattgcga taatcttccc aaggatgcca
      661 gtcgggtaaa tactgtccta catggtattg ataagaaatt tttttattat gcgtcacttt
      721 agacttaata tattgaattt cttttctctt caaaaat
//
LOCUS       DAJSBM010000215          729 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJSBM010000215 DAJSBM010000000
VERSION     DAJSBM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.234"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..729
                     /locus_tag="ONT67_002991"
     rRNA            <1..729
                     /locus_tag="ONT67_002991"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          180 a          162 c          229 g          158 t
ORIGIN      
        1 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
       61 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      121 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      181 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      241 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      301 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      361 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      421 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      481 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      541 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      601 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      661 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      721 gaggactta
//
LOCUS       DAJSBM010000216          714 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJSBM010000216 DAJSBM010000000
VERSION     DAJSBM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.235"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..448)
                     /locus_tag="ONT67_002992"
     CDS             complement(<1..448)
                     /locus_tag="ONT67_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356650.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT          216 a          124 c           95 g          279 t
ORIGIN      
        1 tcaactttct cttttcaaaa aaaatttgat atttttttag atttcttttt agtctttgta
       61 atactttata agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga
      121 tatatttttc ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac
      181 gagataacag agctttgcta gaacattttc cattaatttc cagacatata tccaattcac
      241 tgacggttac tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat
      301 cgatcgtttt tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg
      361 actctgataa atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt
      421 ctttgcttaa aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt
      481 ccatgcattc accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt
      541 cagcgattct tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc
      601 gttttatttg gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat
      661 gcatttttat taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DAJSBM010000217          707 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJSBM010000217 DAJSBM010000000
VERSION     DAJSBM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.236"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            192..>707
                     /locus_tag="ONT67_002993"
     CDS             192..>707
                     /locus_tag="ONT67_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356651.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDL"
BASE COUNT          243 a           71 c          116 g          277 t
ORIGIN      
        1 tgagaagctg aaatttactt ttatttgatg actggaaata gaaattaaat atattgtatt
       61 gttacattaa agaaaacttg tatggaaaaa tcagtacaat accattattt aatgttcttc
      121 acttaaagcg caaaaaatat cagtttatta aactcgtata tagaacaaat atatgtagac
      181 ggggcgaaac gatgagtgta aaaaaaatgc tatttatggt aagtacaata attgtattaa
      241 catcccgatt ttggggaatt aatactagtt tatctatgcg atattttatt atggcaatat
      301 gggatttata ttttatgtta agctattttt cttatcgtga gaaaacatat aaaaaatacg
      361 ttatcaagaa taacttttgg ttgactataa tgccgtttgt gatattttct atctatactc
      421 taataatatg gggaattggt tcaaatgcag aatttgaaaa ttttataaag ttatgttcca
      481 cgttgcttta tttaattctt gcatatggtt acgcatgtac agcattttac ttatttaagg
      541 cagaaggaat tgattttatt ttttggtcag gtgttatttc atatatgagt ggttcagttt
      601 tttatttaat tgtatcttat ggtttaaatg gtttaatttc ttatataaaa agtcttataa
      661 caggtgaaac aaatactgca aattttgcta tggaggtaca tgattta
//
LOCUS       DAJSBM010000218          695 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJSBM010000218 DAJSBM010000000
VERSION     DAJSBM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.237"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(53..628)
                     /locus_tag="ONT67_002994"
     CDS             complement(53..628)
                     /locus_tag="ONT67_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR2356652.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DAJSBM010000219          649 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJSBM010000219 DAJSBM010000000
VERSION     DAJSBM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.238"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>649
                     /locus_tag="ONT67_002995"
     CDS             <1..>649
                     /locus_tag="ONT67_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCR2356653.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DAJSBM010000220          637 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJSBM010000220 DAJSBM010000000
VERSION     DAJSBM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.239"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(107..511)
                     /locus_tag="ONT67_002996"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="ONT67_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          202 a          121 c           99 g          215 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaa
//
LOCUS       DAJSBM010000221          635 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJSBM010000221 DAJSBM010000000
VERSION     DAJSBM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.240"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..270)
                     /locus_tag="ONT67_002997"
     CDS             complement(<1..270)
                     /locus_tag="ONT67_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356654.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="ONT67_002998"
     CDS             complement(276..>635)
                     /locus_tag="ONT67_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCR2356655.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DAJSBM010000222          538 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJSBM010000222 DAJSBM010000000
VERSION     DAJSBM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.241"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          167 a          111 c           76 g          184 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacag
//
LOCUS       DAJSBM010000223          528 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJSBM010000223 DAJSBM010000000
VERSION     DAJSBM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.242"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(5..90)
                     /locus_tag="ONT67_002999"
     tRNA            complement(5..90)
                     /locus_tag="ONT67_002999"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="ONT67_003000"
     tRNA            complement(107..178)
                     /locus_tag="ONT67_003000"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="ONT67_003001"
     tRNA            complement(190..262)
                     /locus_tag="ONT67_003001"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="ONT67_003002"
     tRNA            complement(280..361)
                     /locus_tag="ONT67_003002"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="ONT67_003003"
     tRNA            complement(369..441)
                     /locus_tag="ONT67_003003"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="ONT67_003004"
     tRNA            complement(445..517)
                     /locus_tag="ONT67_003004"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAJSBM010000224          526 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJSBM010000224 DAJSBM010000000
VERSION     DAJSBM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.243"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT67_003005"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="ONT67_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR2356656.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="ONT67_003006"
     CDS             complement(349..>526)
                     /locus_tag="ONT67_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356657.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DAJSBM010000225          524 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJSBM010000225 DAJSBM010000000
VERSION     DAJSBM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.244"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            1..524
                     /locus_tag="ONT67_003007"
                     /pseudo
     CDS             1..524
                     /locus_tag="ONT67_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          218 a           91 c           95 g          120 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aaca
//
LOCUS       DAJSBM010000226          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJSBM010000226 DAJSBM010000000
VERSION     DAJSBM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.245"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          228 a           70 c           82 g          129 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactc
//
LOCUS       DAJSBM010000227          509 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJSBM010000227 DAJSBM010000000
VERSION     DAJSBM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.246"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..132)
                     /locus_tag="ONT67_003008"
     CDS             complement(<1..132)
                     /locus_tag="ONT67_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR2356658.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAJSBM010000228          508 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJSBM010000228 DAJSBM010000000
VERSION     DAJSBM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.247"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            7..402
                     /locus_tag="ONT67_003009"
     CDS             7..402
                     /locus_tag="ONT67_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356659.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT          225 a           48 c           84 g          151 t
ORIGIN      
        1 aaggacatga tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat
       61 aattctggat gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta
      121 ataactgtca agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa
      181 cttcctgagt ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa
      241 aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc
      301 aaagaaaaaa agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt
      361 tttaacccta aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt
      421 aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa
      481 gaaaaactgg ataactagtt tatttaat
//
LOCUS       DAJSBM010000229          493 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJSBM010000229 DAJSBM010000000
VERSION     DAJSBM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.248"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..365
                     /locus_tag="ONT67_003010"
     CDS             <1..365
                     /locus_tag="ONT67_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356660.1"
                     /translation="VFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKTM
                     FYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGGK
                     NDYKDEIVYVYDYEKGDK"
BASE COUNT          196 a           35 c           84 g          178 t
ORIGIN      
        1 tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca tttgggttta
       61 tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt ttaagacctg
      121 atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat caagacaaag
      181 gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt ggttgttctg
      241 aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat gaaaaattag
      301 gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa aaaggtgata
      361 aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat gatgattttc
      421 ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat ggtagagata
      481 aaaaatatac att
//
LOCUS       DAJSBM010000230          487 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJSBM010000230 DAJSBM010000000
VERSION     DAJSBM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.249"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>487)
                     /locus_tag="ONT67_003011"
     CDS             complement(<1..>487)
                     /locus_tag="ONT67_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR2356661.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DAJSBM010000231          486 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJSBM010000231 DAJSBM010000000
VERSION     DAJSBM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.250"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..>486)
                     /locus_tag="ONT67_003012"
     CDS             complement(<1..>486)
                     /locus_tag="ONT67_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356662.1"
                     /translation="ILIFMGLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICAT
                     VISLVFIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKY
                     FFNLYLSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLK
                     TG"
BASE COUNT          191 a           98 c           48 g          149 t
ORIGIN      
        1 cctgttttca atccaaattt tttagttagt cttatcggtt tcacaaaaaa taaccaaaac
       61 atccatataa taaaaactat acatccaact tctatataaa tagttaatat atcactgtgt
      121 aggcttgctg caattcttcc accatttaac ctaaagtgcg ataaataaag attaaaaaag
      181 tattttgaaa accatgtcca tcctgttcct acatatccta aaccaagttc ataaaattta
      241 gatgaatata gataaaatga aagcctcgca ccgtcatctg taatgcccaa atttgacata
      301 agcaatgcca aaattcctgt atctataatg taaataaaca ctaacgaaat aacagttgca
      361 caaatagtac acactaatgc acggatttga atcttttttc cccactttaa taaaacataa
      421 tacactagaa ttgcaccact taaagctaac agttcaattc tcttcaatcc catgaaaatt
      481 aaaatt
//
LOCUS       DAJSBM010000232          462 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJSBM010000232 DAJSBM010000000
VERSION     DAJSBM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.251"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..430)
                     /locus_tag="ONT67_003013"
     CDS             complement(<1..430)
                     /locus_tag="ONT67_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR2356663.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAJSBM010000233          460 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJSBM010000233 DAJSBM010000000
VERSION     DAJSBM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.252"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(20..91)
                     /locus_tag="ONT67_003014"
     tRNA            complement(20..91)
                     /locus_tag="ONT67_003014"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ONT67_003015"
     tRNA            complement(113..200)
                     /locus_tag="ONT67_003015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ONT67_003016"
     tRNA            complement(225..298)
                     /locus_tag="ONT67_003016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ONT67_003017"
     tRNA            complement(312..382)
                     /locus_tag="ONT67_003017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ONT67_003018"
     tRNA            complement(384..459)
                     /locus_tag="ONT67_003018"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAJSBM010000234          436 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJSBM010000234 DAJSBM010000000
VERSION     DAJSBM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.253"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            227..424
                     /locus_tag="ONT67_003019"
     CDS             227..424
                     /locus_tag="ONT67_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2356664.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           61 c           78 g          139 t
ORIGIN      
        1 ttacgtaaat ggaaagtttt gtctcgctta atttgacaaa taataatatt taaaccgtat
       61 atcgagtgat tatccttgtg ttagcattac atccgtgtta tagtaaataa gtaatctaat
      121 ttgaaacgta atctgagcga tatattcaca ctataaaaac tccttttacc aagtaatatt
      181 aattgcaata aaacacgtat tatatacgta tcaggaggaa atatatatga ataacggtac
      241 agtaaaatgg tttaactcag ataaaggttt tggtttcatc actggtgaag atggaaatga
      301 cgtattcgct catttctcag caatccaagg agaaggcttt aagtctttag atgaaggcca
      361 agcagttact tatgatattg aagaaggaca acgtggtcct caagcagtaa atatcgtaaa
      421 ataatgttga acgtta
//
LOCUS       DAJSBM010000235          415 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJSBM010000235 DAJSBM010000000
VERSION     DAJSBM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.254"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..339)
                     /locus_tag="ONT67_003020"
     CDS             complement(<1..339)
                     /locus_tag="ONT67_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR2356665.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAJSBM010000236          409 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJSBM010000236 DAJSBM010000000
VERSION     DAJSBM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.255"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..379
                     /locus_tag="ONT67_003021"
     CDS             <1..379
                     /locus_tag="ONT67_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCR2356666.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DAJSBM010000237          404 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJSBM010000237 DAJSBM010000000
VERSION     DAJSBM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.256"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>404
                     /locus_tag="ONT67_003022"
     CDS             <1..>404
                     /locus_tag="ONT67_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356667.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DAJSBM010000238          381 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJSBM010000238 DAJSBM010000000
VERSION     DAJSBM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.257"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(21..94)
                     /locus_tag="ONT67_003023"
     tRNA            complement(21..94)
                     /locus_tag="ONT67_003023"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="ONT67_003024"
     tRNA            complement(108..181)
                     /locus_tag="ONT67_003024"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="ONT67_003025"
     tRNA            complement(209..281)
                     /locus_tag="ONT67_003025"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="ONT67_003026"
     tRNA            complement(301..374)
                     /locus_tag="ONT67_003026"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAJSBM010000239          343 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJSBM010000239 DAJSBM010000000
VERSION     DAJSBM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.258"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
BASE COUNT          126 a           57 c           54 g          106 t
ORIGIN      
        1 attagcgatg cagggatcga gagaaacatc aaaagggtat ccttcaagat cacgttaaac
       61 aagcctaccg ataaaaagct ctcagaagcg ttctgagagc ttttgacatt ttcttgtata
      121 attttatcct aacacattac aaagcaccta attcgagctt atatcaagca aatttgctat
      181 ttattactca atacatatct aaaaattact tgatggaaaa ttagatacat ggtaagcatg
      241 aaaagaaaat caatttgagc agatgcagta caatacatag caatttaaaa gctctcagaa
      301 ggcttctgag agcttttaat atattctttg ataatttgtc ttt
//
LOCUS       DAJSBM010000240          341 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJSBM010000240 DAJSBM010000000
VERSION     DAJSBM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.259"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..>341
                     /locus_tag="ONT67_003027"
     CDS             <1..>341
                     /locus_tag="ONT67_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR2356668.1"
                     /translation="RLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQMLEQ
                     NTELNEYVATLEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITRL
                     SIRFGLAEKDMG"
BASE COUNT          120 a           50 c           76 g           95 t
ORIGIN      
        1 aggcttcctg atggttcgta tcatttatct gggaatgatt ttgttgattt gtatcaacga
       61 gctacgagag caacaaatgc caagagaaat gctgataaca tgagtgagca atatttacag
      121 atgcttgagc aaaatacaga attaaatgag tatgtagcta cattggaaga agagcgtcat
      181 ttgtctggta gaaaacaatt agcagattta caagtggaaa ataaaaagct gaaagaagaa
      241 aatcgctctt tacgccagcg tttagagcga ttaaaaaacg catttcaaaa atcgatcact
      301 cgtttgtcta tccgttttgg tcttgcagaa aaggatatgg g
//
LOCUS       DAJSBM010000241          339 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJSBM010000241 DAJSBM010000000
VERSION     DAJSBM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.denovo.260"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..321)
                     /locus_tag="ONT67_003028"
     CDS             complement(<1..321)
                     /locus_tag="ONT67_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR2356669.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           95 a           55 c           61 g          128 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttct
//
LOCUS       DAJSBM010000242        13213 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.071, whole genome shotgun sequence.
ACCESSION   DAJSBM010000242 DAJSBM010000000
VERSION     DAJSBM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.071"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(204..2162)
                     /locus_tag="ONT67_003029"
     CDS             complement(204..2162)
                     /locus_tag="ONT67_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2356670.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="ONT67_003030"
     CDS             complement(2159..3031)
                     /locus_tag="ONT67_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR2356671.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="ONT67_003031"
     CDS             complement(3373..4245)
                     /locus_tag="ONT67_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR2356672.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="ONT67_003032"
     CDS             4373..4942
                     /locus_tag="ONT67_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCR2356673.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="ONT67_003033"
     CDS             5009..5746
                     /locus_tag="ONT67_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR2356674.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="ONT67_003034"
     CDS             5977..7245
                     /locus_tag="ONT67_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCR2356675.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="ONT67_003035"
     CDS             7732..8196
                     /locus_tag="ONT67_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR2356676.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="ONT67_003036"
     CDS             8180..8809
                     /locus_tag="ONT67_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCR2356677.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="ONT67_003037"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="ONT67_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCR2356678.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="ONT67_003038"
     CDS             10688..11134
                     /locus_tag="ONT67_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCR2356679.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="ONT67_003039"
     CDS             complement(11333..11848)
                     /locus_tag="ONT67_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCR2356680.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="ONT67_003040"
     CDS             11965..13167
                     /locus_tag="ONT67_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR2356681.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAJSBM010000243         9839 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.084, whole genome shotgun sequence.
ACCESSION   DAJSBM010000243 DAJSBM010000000
VERSION     DAJSBM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.084"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(122..319)
                     /locus_tag="ONT67_003041"
     CDS             complement(122..319)
                     /locus_tag="ONT67_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356682.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(414..2174)
                     /gene="efrB"
                     /locus_tag="ONT67_003042"
     CDS             complement(414..2174)
                     /gene="efrB"
                     /locus_tag="ONT67_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2356683.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(2171..3901)
                     /gene="efrA"
                     /locus_tag="ONT67_003043"
     CDS             complement(2171..3901)
                     /gene="efrA"
                     /locus_tag="ONT67_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2356684.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4294..4506
                     /locus_tag="ONT67_003044"
     CDS             4294..4506
                     /locus_tag="ONT67_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2356685.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            4508..6187
                     /locus_tag="ONT67_003045"
     CDS             4508..6187
                     /locus_tag="ONT67_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2356686.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6441..6641)
                     /locus_tag="ONT67_003046"
     CDS             complement(6441..6641)
                     /locus_tag="ONT67_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2356687.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(6791..6934)
                     /locus_tag="ONT67_003047"
                     /pseudo
     CDS             complement(6791..6934)
                     /locus_tag="ONT67_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(7169..7831)
                     /locus_tag="ONT67_003048"
     CDS             complement(7169..7831)
                     /locus_tag="ONT67_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2356688.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            8223..8282
                     /locus_tag="ONT67_003049"
     CDS             8223..8282
                     /locus_tag="ONT67_003049"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2356689.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            8340..9071
                     /gene="erm(A)"
                     /locus_tag="ONT67_003050"
     CDS             8340..9071
                     /gene="erm(A)"
                     /locus_tag="ONT67_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2356690.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGTGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(9197..>9839)
                     /gene="ant(9)"
                     /locus_tag="ONT67_003051"
     CDS             complement(9197..>9839)
                     /gene="ant(9)"
                     /locus_tag="ONT67_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)"
                     /protein_id="HCR2356691.1"
                     /translation="VDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVI
                     NRSEVVPWQYPPKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPD
                     SSSILVSVPLTDIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAA
                     EWAIPLLPKEHVTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT         3129 a         1990 c         1644 g         3076 t
ORIGIN      
        1 actaatccta ttgaataaag gtatcatagc atatatttat ttcataagaa aaaaaacagg
       61 taaaacctgc tcaggccaga tgtctattta cagtatctgg taactgacgt tttacctgtt
      121 tttattgtac atctatattt tcttctatta ctttttttaa aattaattcg tacacttcta
      181 tttgtgctgc caagcgttcg atttgaaact cattttcaaa aaaatcagct gaatcaaaag
      241 gcttcgtctg acagacgtgc gttaaacgat caacttctct tttggcttca gtaatttttt
      301 catttaaaaa ctgttccatc tccgtgtcct cacttttctt gataacttta ctttaccatt
      361 atcaagaata gagaacaaaa aataagtttt aaactgtttt attcttatat gacttattct
      421 gcttcctcag caaattggct gttatacagt ttttcataaa atccaccttg tgccaataaa
      481 ctctcatgcg ttcctttttc aatgatctga ccttgatcca tcactagaat cagatctgca
      541 tcacggattg tcgataaacg gtgagcgatg acgaaacttg tccgtccctc catcacgcga
      601 tccattgctt tttgaatcaa cgcttctaaa cgggtgtcta ctgaacttgt tgcttcatct
      661 aaaatcaaga ttttaggatc ggagaccact gctcgggcaa tcgtcagcaa ctgtttttgt
      721 cctaaagaca cattgtctcc ctcagaattg atggccatat catatccatc tggcatcgtt
      781 cggataaaat gatctacgtt tgctgttttc gcagcatcaa ctacttcata atctgtcgca
      841 tcaagctttc cgaagcggat attatctgca atcgttcctt gataaagcca tgcatcttgc
      901 aagaccatac caaatagcga acgcacatca ctgcgactca ttgatttggt gtcgattcca
      961 tcaatcctta ttgctccttt gtctacgtca taaaaacgca tcaatagatt gatcaatgtc
     1021 gtcttgccgg caccggtcgg accgaccaca gctacggttt gaccagcttt cacttcaaaa
     1081 ttcaaatctt ggattaatgg tttcttcgga tcatatgcaa aatcgacatg atcgaatgtc
     1141 acatctccta aaatcgtttc tggcagtgaa gcatctactt tgttttcttt ttcttctggc
     1201 tcatctaaaa tctcgaatac acgtttcgtt gccgctgatg cactttgcaa aactgaagac
     1261 aattgtgtca cgttccccat tggctgactg atttgccaaa tatattggat gaatgcttgt
     1321 agttgaccga caacgatcgc cccagtaata acataaaagc ttcctagaac agccatacca
     1381 atataagtag cataggcagt taattgaact agaggcatca tcaagccaga aatgaatgct
     1441 gcacgaaacc caaagtattt caagctgtga ttaacttttt tgaatccttc caatgtttct
     1501 ttttcgcgtc catatacttt caatacacta aaacctgtca tattttcttg gacgtagccg
     1561 tttagctcac caagagaatt ctgcatgctt tgaaaatatt tctgcgaagc attgatcact
     1621 cgttttgaga tccagatcga tgttggaatc atgatcatag agatcagcgc catcatcggc
     1681 tggatataaa acatcattgc tgcagccaac gtaattccca atatcgcatt tacgatacca
     1741 ataaaagctt gttgtaaagc accgctgact gcatctacat cattcgtcac acgagacaaa
     1801 atatttcctt gctggttttt atcgaaatat gaaaccggaa gacgattgat tttttcttct
     1861 atgtctcttc tcaaatcatg catggcactt tgaacgacat ttgtcatcaa aacaccagaa
     1921 agtaattgcg aaatacaata ccctattcca acaactgtga tccaaatgag acatttgaat
     1981 atataagaaa aattgattga ttcgccattg gcaatattac ggctgatttc tgtagtagga
     2041 agtcctgtaa catatggcaa agccacgttg aaagccactg tcagaactgt aaataggatc
     2101 actaaaagaa aagtctcacg atacggtctg atatatcgag ctaaacgttt taaggaagaa
     2161 aaggtttttt tcatgctaat tcctcctttg ataattgaga agcggcaata tcataataaa
     2221 tcgggcattt ttccaacaac tcccgatgcg tccccatacc gacgacttct ccttcattta
     2281 atacgatgat cttatctgca tgcatgatcg ttcccacacg ttgtgctacg atcaatacag
     2341 tagattctgt cgtttctttt ttcagacgag cacgtaactt cgcatccgtt tgataatcta
     2401 atgcagaaaa gctgtcatca aaaatataga tttccggacg gcgtatgatg gctctggcta
     2461 ttgccaatcg ttgtttttgt ccacctgaaa aattggaacc accttctgat aaatgttcat
     2521 taaatccatc tggttttcga gaaatgaatt ctgaagcttg agcaatctca gctgcacgtt
     2581 ccatttcttc ttgcgtagca tcttctttcc cgtatcgcaa attatctgca atcgttccag
     2641 taaatagcag cgctttttgc ggaataaacc caatcttatc tcgcaacttg cttagccgat
     2701 aatctctcac gtctactcca tcgatcaaga tctttccttc actcacatca taaaagcgag
     2761 ggatcaattg gatcaacgta gattttccgc taccggtact tccaataaaa gcaacggtct
     2821 ctcctggaga ggctgtaaaa ctgacatttc gtatcactgg ctcttgcgaa tggcctggat
     2881 aagcaaatgt tacattttga aattcgatat accctttagt ttttgtatca gatataccat
     2941 ttggatcttc tacaatcgaa ggtttggcat ctagggcttc ttggatacgt ccagctgaaa
     3001 cagctgctct tggatacatc atgaacacac tagcaaataa catgaatgag aacaaggcat
     3061 ggaatatata ctcgataaac gcaatcaaat ctcctacacg aagctcgcct gcatcgattt
     3121 gcactgcccc gctccagatg atcaatacca ttacgatatt aaataagaaa taaaaacccg
     3181 gttgtgcaac agccatcaaa cggaataagc tttttgaact agaagcataa ttctcattga
     3241 ctacagcaaa acgtttttct tcaaactttt cattgacgaa tgcacgaatc acacgcaatc
     3301 ctgaaagatt ttcccgcaag atactgttga ttttatctaa gtttttttgt tgctttttag
     3361 ataacggttc agacacctta gcaatcgcta cgactgccaa taatagtaaa ggtaatgcac
     3421 ctactacata caaactgagt gtcacacttg tacgcatgac catgtacaaa ctgacaatga
     3481 acatcattgg tgccataaaa ccgatacgta aaatattttg caggaacaac atgatctgat
     3541 aagcatcgtt tgttgtacga gtgatcaatg aagatacgcc tagattttcg tattcctgat
     3601 gcgaataact ttggatcttt tcgaacaaat catctcgaat atctgctacg atattagtcg
     3661 taatcctcga accaaaataa cctaacaaga tattcaaaat aataccgatt acagtaataa
     3721 cgatcataaa tatcccttgt tgcttcacat aatcaaaatc atcattgcgg attcctacat
     3781 cgatcatacg tgctaaaatc gtaggaagtc ctaattcaat caaaataaaa ccaaatacgc
     3841 atatgaaatt gaaaaacaag aatttcttat aacgcatggt ataatgccac attagtttca
     3901 ctttctcatc tccttttaat cttttgttac attttcatta ctaaaaatga tagcacaacg
     3961 agcctattaa tagaacactt ctctcataaa aaagtcatta tgattatagc gggatgataa
     4021 acattgattt tttctttcaa tctagttgat ttctatactt atttgctgtt ttatgctaga
     4081 ataacaatta attgttgtat attttttata cagaaggtac ggttgaaaaa atatccgaac
     4141 agataagcaa aaacgaggct tgtctctttg actcgaatcc agtattctag tcaaagcata
     4201 tgaaaaacca ggtggcttgt cccctcgttt tgtgttgtct aaaaaaatca actagctaaa
     4261 tcactgcttt acgcagataa aggagacaaa acaatgatct ataaagttta ttatcaagaa
     4321 acaaaagtac gcaaccctaa acgggaagaa acaaaatctc tttacattga ggcaaaaagc
     4381 gatgtagacg ctcgccaacg tgtggaagaa aataccccat acaatattga attcgttcaa
     4441 cttctagaag gaagtcatct agagtacgaa aaagaaaatg ctgatttctc attagtggag
     4501 ttttaatatg aaactttcca tcaaaaataa cgagacaggc gtctttgcta ttggtggatt
     4561 aggtgaaatc gggaaaaata cgtatggggt acaattccaa gatgaaatca ttttgattga
     4621 tgccgggatc aaattcccgg aagacgattt acttggaatt gactatgtca ttcccgacta
     4681 cagctacatc atacaaaact tacacaaagt aaaagccttg gtgattactc acgggcacga
     4741 agaccacatc ggcggaatac cctacttgct gaaacaagca aatattccta tctatgctgg
     4801 tcctctcgca ttagcgttga tcacaaacaa attagatgag catggcttgc ttcgagatgc
     4861 agagctccat gagatcaatg aagatacgat catccgtttt agaaagactt ctgtcagctt
     4921 tttcagaacg acccatagta tcccagatgc attagggatc gtggtgaaaa caccttctgg
     4981 aaatatcgta gcgactggag acttcaaatt cgatttcact cctgttggcg aaccagcaaa
     5041 cttgcataaa atggctcgta tcggggaaga aggagtcttg tgccttttat ctgatagtac
     5101 aaatgctgag attccaacat ttactaaatc agaaaaaacg attgggcatt ctattttgaa
     5161 gatcttttca aaaatcgaag gacgtatcat ttttgccagc tttgcttcta acatcttccg
     5221 tttgcagcaa gcagcagatg ccgctgtggc aactggtcgt aaaatcgctg ttttcggacg
     5281 aagcatggaa aatgccatcg tcaacggtca acgtttagga tatatcaaag ttccagacgg
     5341 cacatttgta gatgcacacg aaatcaatcg cttgcctgcc aatgaaacga tgatcctatg
     5401 tacaggttct caaggagaag cgatggccgc gttgagccgc atcgcgaatg gtactcaccg
     5461 tcaaatccaa atccagccag acgatacagt gatcttttca agttcaccta ttcctggtaa
     5521 tacaacaagt gtcaatcatt tgatcaattt gctgtctgaa gctggtgcag aagtgattca
     5581 cggaaaaatc aataacatcc atacttccgg tcacggtgga caagaagaac aaaaattaat
     5641 gcttcgtttg atgaagccaa aatattttat tcctgtccac ggagaattcc gcatgttgaa
     5701 agtacatgct tctctagcac aagatgtagg tgttcctgca gaaaactgct tcatcatgga
     5761 aaatggcgat gttttagcgt taactagcga ttctgcgcgt cctgctgggc attttgtggc
     5821 tggagatgtg tatatcgatg gtagcgggat cggtgatatt ggtaatgtcg ttttacgtga
     5881 ccgtcacctg ctttcagaag aaggtcttgt attagctgtc gcgacagtgg atatcaagaa
     5941 aaaagaaatc ttggctggtc cagatattct ctctcgtgga tttgtctaca tgcgagaatc
     6001 aggagaaatg atcaatgaag ctcagcgaat cttattccac gctttaagag aatgtttgaa
     6061 tggacctgac tgttcagaat ataaattgaa agaagcgatg actggcgcgc ttcagccttt
     6121 cttattcgaa cgtacagaaa gacatccaat gattttgcca atggtaatga caccagatga
     6181 agaataaata agaggttatg aaatcagcac cttgtcctga atataagaga aaacaaatga
     6241 aaaacatgag aagccatttt tgtttgttct tattccttga tacaaggtgg ctctttcata
     6301 gcctcttaaa tttatttact ttttcataca aaaaaatacc tgccacaaga gaaattgtct
     6361 cttgtggcag gtatttttgt tactcgcttt gttctctcgt aaaaattgat gtctaccatc
     6421 aatttgattt acagataaaa ttaagctttt acaacgtttg cagcttgtgg gccgcggtct
     6481 gattcttcaa cgtcgaaagt cactgcttga ccttcttcta atgttttgaa tccgtcacct
     6541 tggatagctg agaagtgaac gaatacgtcg cttccatctt cacgtgagat aaatccaaat
     6601 cctttttcag cgttgaacca ttttactgtt ccgttttcca taataatatc ctcctcgtgc
     6661 caaaagcact aatttaaatt gtaacattgc tcgtgtacga tagaggtgca acagatgttt
     6721 caacttctat tcccaaacaa aattacaata taattgtaca cttgcttcat tttttttgca
     6781 agaaagcaac ttacttctct tccatcattc cttcttctct caaactaaaa tatcgtttgc
     6841 gtccgatgat caagtggtcc agtacagtga tccccatcat ttctccacac ttttgtatcc
     6901 ttttggtaaa attcatatcc tgctgcgatg gaatttcata ttatcaagag taaattacaa
     6961 aagaatcatt taaatcaata ctttcacttt gaatacatgt atttgaagtg gaaggtactt
     7021 atttcaaaaa tagtaaaacc tgtatcttaa attacttaat agtaacataa gatacaggct
     7081 gattttttat tcattgttat ttatactaaa gcacccgata gctctgaaaa caatcacaaa
     7141 ttcaactttt caaagccaca gctttaagtt attttgtcca gacaaccccc attgcccaac
     7201 ccattttatg gaattggcat ccaggcaaca acttttcata taaatcgtaa taattttgtt
     7261 cagataggta cttatctgaa gctaaatgct caagccatga tttagatgtg ttgtgattat
     7321 aaattctaat cgcatttttt attccaaatt tgaaacaatg cggtaaaaac tcttggattg
     7381 cccctaattt ataaacatag gtaggcggtg tctcgacttc agatacatta tcaagaataa
     7441 ctattcttcc ttcctcattc aacagttcct tcatttgctg tattacgctg gctatatcat
     7501 ccaaatgatg aaaggttgtt cggcttacaa taaaatcaaa cttctcatta aaattaagtt
     7561 gttctgcatt catattcaga tagaccgtat ttgttagttg acgtttagat ttggcaagat
     7621 cgagcatttg attagaaata tcaatcccta ccacttcatc ataataactt gctaatttct
     7681 ccactaacaa acccgagcca catccgatat ctaatgctct gcctttcttt ggagacatat
     7741 tagacacaaa gaatgaataa tcattcaaaa gctcattcac gaaatcgtaa tcttctgcaa
     7801 ccttatcaaa ctgtgattct attgtattca aaaagatccc ccattcctac tttatcgaca
     7861 ttctttcatt acttaccact ttagatgttt tttcgttggg gataaaactt ccctttagac
     7921 aattttatcc aaagacaata caacagtgca actttattaa agtcactgtc ctttatcgca
     7981 gcctttactt tttagtaaag acagtggctt ctcttatcaa gtttcaaaac atattatttt
     8041 gaagaaaacg tccatctgaa gtgtcaagtg caaaattaca tataaaggtt tattctaaaa
     8101 tgaaaagatg atacaatcat attcagttac ataaggaggt ttcaattatg tgcaccagta
     8161 tcgcagtagt agaaattact ttatctcatt cataatgaaa aaaatggaaa ggagataaaa
     8221 gtatgggtac tttttctata tttgttatta ataaagttcg ttatcaacca aatcaaaatt
     8281 aattggttat aatgaacgct taatgtcagt tcattataac cagtaaggag aaggttataa
     8341 tgaaccagaa aaaccctaaa gacacgcaaa attttattac ttctaaaaag catgtaaaag
     8401 aaatattgaa tcacacgaat atcagtaaac aagacaacgt aatagaaatc ggaacaggaa
     8461 aaggacattt taccaaagag ctagtcaaaa tgagtcgatc agttactgct atagaaattg
     8521 atggaggctt atgtcaagtg actaaagaag cggtaaaccc ctctgagaat ataaaagtga
     8581 ttcaaacgga tattctaaaa ttttccttcc caaaacatat aaactataag atatatggta
     8641 atattcctta taacatcagt acggatattg tcaaaagaat tacctttgaa agtcaggcta
     8701 aatatagcta tcttatcgtt gagaagggat ttgcgaaaag attgcaaaat ctgcaacgag
     8761 ctttgggttt actattaatg gtggagatgg atataaaaat gctcaaaaaa gtaccaccac
     8821 tatattttca tcctaagcca agtgtagact ctgtattgat tgttcttgaa cgacatcaac
     8881 cattgatttc aaagaaggac tacaaaaagt atcgatcttt tgtttataag tgggtaaacc
     8941 gtgaatatcg tgttcttttc actaaaaacc aattccgaca ggctttgaag catgcaaatg
     9001 tcactaatat taataaacta tcgaaggaac aatttctttc tattttcaat agttacaaat
     9061 tgtttcacta aattaaagta ataaagcgtt ctctaatttc acaagaggac gctttattct
     9121 tcccaaaaat tgttcaatat ttatcaataa atcagtagtt ttaaaagtaa gcacctgtta
     9181 ttgcaataaa attagcctaa ttgagagaag tttctataga atttttcata tacttaacga
     9241 gtgctttcac ctttgaatat agtccttccc acttatcatc acactctccc cgatagcctt
     9301 ttctagctat atccagtaaa gttacatgct ctttaggtaa aagaggtata gcccattctg
     9361 cagcgacatc tttcgaggta atttcaccag tagtcactgt ttgccacatt cgagctaggg
     9421 ttaaaattac attacgctca tcacctttta tcccctcaat tagttctggc aaagaatcct
     9481 taattgctct tcgaatatct gtcaaaggta cggagacaag tatacttgaa gaatcaggac
     9541 caaatagaga aatactattc tttcttgctt gtgctaaaac aatagccaaa tcaggatcat
     9601 agcttggttc ctgaatttgt ccattctcaa attcacccct gagccactca ccgtatataa
     9661 attctctttt tggaggatat tgccaaggga caacttcact cctatttata accgtaactt
     9721 caagtggtct aacagaatcc gtatttccaa tctttcctga tatagtcatt agtctttctg
     9781 ttagtttttt tcgagttaat tgaggtaaac tatgattcac gacgactaga acatctaca
//
LOCUS       DAJSBM010000244         9839 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.085, whole genome shotgun sequence.
ACCESSION   DAJSBM010000244 DAJSBM010000000
VERSION     DAJSBM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.085"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(122..319)
                     /locus_tag="ONT67_003052"
     CDS             complement(122..319)
                     /locus_tag="ONT67_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356692.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(414..2174)
                     /gene="efrB"
                     /locus_tag="ONT67_003053"
     CDS             complement(414..2174)
                     /gene="efrB"
                     /locus_tag="ONT67_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCR2356693.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(2171..3901)
                     /gene="efrA"
                     /locus_tag="ONT67_003054"
     CDS             complement(2171..3901)
                     /gene="efrA"
                     /locus_tag="ONT67_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCR2356694.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4294..4506
                     /locus_tag="ONT67_003055"
     CDS             4294..4506
                     /locus_tag="ONT67_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCR2356695.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            4508..6187
                     /locus_tag="ONT67_003056"
     CDS             4508..6187
                     /locus_tag="ONT67_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCR2356696.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6441..6641)
                     /locus_tag="ONT67_003057"
     CDS             complement(6441..6641)
                     /locus_tag="ONT67_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR2356697.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(6791..6934)
                     /locus_tag="ONT67_003058"
                     /pseudo
     CDS             complement(6791..6934)
                     /locus_tag="ONT67_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(7169..7831)
                     /locus_tag="ONT67_003059"
     CDS             complement(7169..7831)
                     /locus_tag="ONT67_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR2356698.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            8223..8282
                     /locus_tag="ONT67_003060"
     CDS             8223..8282
                     /locus_tag="ONT67_003060"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HCR2356699.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            8340..9071
                     /gene="erm(A)"
                     /locus_tag="ONT67_003061"
     CDS             8340..9071
                     /gene="erm(A)"
                     /locus_tag="ONT67_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HCR2356700.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(9197..>9839)
                     /gene="ant(9)"
                     /locus_tag="ONT67_003062"
     CDS             complement(9197..>9839)
                     /gene="ant(9)"
                     /locus_tag="ONT67_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)"
                     /protein_id="HCR2356701.1"
                     /translation="VDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVI
                     NRSEVVPWQYPPKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPD
                     SSSILVSVPLTDIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAA
                     EWAIPLLPKEHVTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT         3128 a         1990 c         1644 g         3077 t
ORIGIN      
        1 actaatccta ttgaataaag gtatcatagc atatatttat ttcataagaa aaaaaacagg
       61 taaaacctgc tcaggccaga tgtctattta cagtatctgg taactgacgt tttacctgtt
      121 tttattgtac atctatattt tcttctatta ctttttttaa aattaattcg tacacttcta
      181 tttgtgctgc caagcgttcg atttgaaact cattttcaaa aaaatcagct gaatcaaaag
      241 gcttcgtctg acagacgtgc gttaaacgat caacttctct tttggcttca gtaatttttt
      301 catttaaaaa ctgttccatc tccgtgtcct cacttttctt gataacttta ctttaccatt
      361 atcaagaata gagaacaaaa aataagtttt aaactgtttt attcttatat gacttattct
      421 gcttcctcag caaattggct gttatacagt ttttcataaa atccaccttg tgccaataaa
      481 ctctcatgcg ttcctttttc aatgatctga ccttgatcca tcactagaat cagatctgca
      541 tcacggattg tcgataaacg gtgagcgatg acgaaacttg tccgtccctc catcacgcga
      601 tccattgctt tttgaatcaa cgcttctaaa cgggtgtcta ctgaacttgt tgcttcatct
      661 aaaatcaaga ttttaggatc ggagaccact gctcgggcaa tcgtcagcaa ctgtttttgt
      721 cctaaagaca cattgtctcc ctcagaattg atggccatat catatccatc tggcatcgtt
      781 cggataaaat gatctacgtt tgctgttttc gcagcatcaa ctacttcata atctgtcgca
      841 tcaagctttc cgaagcggat attatctgca atcgttcctt gataaagcca tgcatcttgc
      901 aagaccatac caaatagcga acgcacatca ctgcgactca ttgatttggt gtcgattcca
      961 tcaatcctta ttgctccttt gtctacgtca taaaaacgca tcaatagatt gatcaatgtc
     1021 gtcttgccgg caccggtcgg accgaccaca gctacggttt gaccagcttt cacttcaaaa
     1081 ttcaaatctt ggattaatgg tttcttcgga tcatatgcaa aatcgacatg atcgaatgtc
     1141 acatctccta aaatcgtttc tggcagtgaa gcatctactt tgttttcttt ttcttctggc
     1201 tcatctaaaa tctcgaatac acgtttcgtt gccgctgatg cactttgcaa aactgaagac
     1261 aattgtgtca cgttccccat tggctgactg atttgccaaa tatattggat gaatgcttgt
     1321 agttgaccga caacgatcgc cccagtaata acataaaagc ttcctagaac agccatacca
     1381 atataagtag cataggcagt taattgaact agaggcatca tcaagccaga aatgaatgct
     1441 gcacgaaacc caaagtattt caagctgtga ttaacttttt tgaatccttc caatgtttct
     1501 ttttcgcgtc catatacttt caatacacta aaacctgtca tattttcttg gacgtagccg
     1561 tttagctcac caagagaatt ctgcatgctt tgaaaatatt tctgcgaagc attgatcact
     1621 cgttttgaga tccagatcga tgttggaatc atgatcatag agatcagcgc catcatcggc
     1681 tggatataaa acatcattgc tgcagccaac gtaattccca atatcgcatt tacgatacca
     1741 ataaaagctt gttgtaaagc accgctgact gcatctacat cattcgtcac acgagacaaa
     1801 atatttcctt gctggttttt atcgaaatat gaaaccggaa gacgattgat tttttcttct
     1861 atgtctcttc tcaaatcatg catggcactt tgaacgacat ttgtcatcaa aacaccagaa
     1921 agtaattgcg aaatacaata ccctattcca acaactgtga tccaaatgag acatttgaat
     1981 atataagaaa aattgattga ttcgccattg gcaatattac ggctgatttc tgtagtagga
     2041 agtcctgtaa catatggcaa agccacgttg aaagccactg tcagaactgt aaataggatc
     2101 actaaaagaa aagtctcacg atacggtctg atatatcgag ctaaacgttt taaggaagaa
     2161 aaggtttttt tcatgctaat tcctcctttg ataattgaga agcggcaata tcataataaa
     2221 tcgggcattt ttccaacaac tcccgatgcg tccccatacc gacgacttct ccttcattta
     2281 atacgatgat cttatctgca tgcatgatcg ttcccacacg ttgtgctacg atcaatacag
     2341 tagattctgt cgtttctttt ttcagacgag cacgtaactt cgcatccgtt tgataatcta
     2401 atgcagaaaa gctgtcatca aaaatataga tttccggacg gcgtatgatg gctctggcta
     2461 ttgccaatcg ttgtttttgt ccacctgaaa aattggaacc accttctgat aaatgttcat
     2521 taaatccatc tggttttcga gaaatgaatt ctgaagcttg agcaatctca gctgcacgtt
     2581 ccatttcttc ttgcgtagca tcttctttcc cgtatcgcaa attatctgca atcgttccag
     2641 taaatagcag cgctttttgc ggaataaacc caatcttatc tcgcaacttg cttagccgat
     2701 aatctctcac gtctactcca tcgatcaaga tctttccttc actcacatca taaaagcgag
     2761 ggatcaattg gatcaacgta gattttccgc taccggtact tccaataaaa gcaacggtct
     2821 ctcctggaga ggctgtaaaa ctgacatttc gtatcactgg ctcttgcgaa tggcctggat
     2881 aagcaaatgt tacattttga aattcgatat accctttagt ttttgtatca gatataccat
     2941 ttggatcttc tacaatcgaa ggtttggcat ctagggcttc ttggatacgt ccagctgaaa
     3001 cagctgctct tggatacatc atgaacacac tagcaaataa catgaatgag aacaaggcat
     3061 ggaatatata ctcgataaac gcaatcaaat ctcctacacg aagctcgcct gcatcgattt
     3121 gcactgcccc gctccagatg atcaatacca ttacgatatt aaataagaaa taaaaacccg
     3181 gttgtgcaac agccatcaaa cggaataagc tttttgaact agaagcataa ttctcattga
     3241 ctacagcaaa acgtttttct tcaaactttt cattgacgaa tgcacgaatc acacgcaatc
     3301 ctgaaagatt ttcccgcaag atactgttga ttttatctaa gtttttttgt tgctttttag
     3361 ataacggttc agacacctta gcaatcgcta cgactgccaa taatagtaaa ggtaatgcac
     3421 ctactacata caaactgagt gtcacacttg tacgcatgac catgtacaaa ctgacaatga
     3481 acatcattgg tgccataaaa ccgatacgta aaatattttg caggaacaac atgatctgat
     3541 aagcatcgtt tgttgtacga gtgatcaatg aagatacgcc tagattttcg tattcctgat
     3601 gcgaataact ttggatcttt tcgaacaaat catctcgaat atctgctacg atattagtcg
     3661 taatcctcga accaaaataa cctaacaaga tattcaaaat aataccgatt acagtaataa
     3721 cgatcataaa tatcccttgt tgcttcacat aatcaaaatc atcattgcgg attcctacat
     3781 cgatcatacg tgctaaaatc gtaggaagtc ctaattcaat caaaataaaa ccaaatacgc
     3841 atatgaaatt gaaaaacaag aatttcttat aacgcatggt ataatgccac attagtttca
     3901 ctttctcatc tccttttaat cttttgttac attttcatta ctaaaaatga tagcacaacg
     3961 agcctattaa tagaacactt ctctcataaa aaagtcatta tgattatagc gggatgataa
     4021 acattgattt tttctttcaa tctagttgat ttctatactt atttgctgtt ttatgctaga
     4081 ataacaatta attgttgtat attttttata cagaaggtac ggttgaaaaa atatccgaac
     4141 agataagcaa aaacgaggct tgtctctttg actcgaatcc agtattctag tcaaagcata
     4201 tgaaaaacca ggtggcttgt cccctcgttt tgtgttgtct aaaaaaatca actagctaaa
     4261 tcactgcttt acgcagataa aggagacaaa acaatgatct ataaagttta ttatcaagaa
     4321 acaaaagtac gcaaccctaa acgggaagaa acaaaatctc tttacattga ggcaaaaagc
     4381 gatgtagacg ctcgccaacg tgtggaagaa aataccccat acaatattga attcgttcaa
     4441 cttctagaag gaagtcatct agagtacgaa aaagaaaatg ctgatttctc attagtggag
     4501 ttttaatatg aaactttcca tcaaaaataa cgagacaggc gtctttgcta ttggtggatt
     4561 aggtgaaatc gggaaaaata cgtatggggt acaattccaa gatgaaatca ttttgattga
     4621 tgccgggatc aaattcccgg aagacgattt acttggaatt gactatgtca ttcccgacta
     4681 cagctacatc atacaaaact tacacaaagt aaaagccttg gtgattactc acgggcacga
     4741 agaccacatc ggcggaatac cctacttgct gaaacaagca aatattccta tctatgctgg
     4801 tcctctcgca ttagcgttga tcacaaacaa attagatgag catggcttgc ttcgagatgc
     4861 agagctccat gagatcaatg aagatacgat catccgtttt agaaagactt ctgtcagctt
     4921 tttcagaacg acccatagta tcccagatgc attagggatc gtggtgaaaa caccttctgg
     4981 aaatatcgta gcgactggag acttcaaatt cgatttcact cctgttggcg aaccagcaaa
     5041 cttgcataaa atggctcgta tcggggaaga aggagtcttg tgccttttat ctgatagtac
     5101 aaatgctgag attccaacat ttactaaatc agaaaaaacg attgggcatt ctattttgaa
     5161 gatcttttca aaaatcgaag gacgtatcat ttttgccagc tttgcttcta acatcttccg
     5221 tttgcagcaa gcagcagatg ccgctgtggc aactggtcgt aaaatcgctg ttttcggacg
     5281 aagcatggaa aatgccatcg tcaacggtca acgtttagga tatatcaaag ttccagacgg
     5341 cacatttgta gatgcacacg aaatcaatcg cttgcctgcc aatgaaacga tgatcctatg
     5401 tacaggttct caaggagaag cgatggccgc gttgagccgc atcgcgaatg gtactcaccg
     5461 tcaaatccaa atccagccag acgatacagt gatcttttca agttcaccta ttcctggtaa
     5521 tacaacaagt gtcaatcatt tgatcaattt gctgtctgaa gctggtgcag aagtgattca
     5581 cggaaaaatc aataacatcc atacttccgg tcacggtgga caagaagaac aaaaattaat
     5641 gcttcgtttg atgaagccaa aatattttat tcctgtccac ggagaattcc gcatgttgaa
     5701 agtacatgct tctctagcac aagatgtagg tgttcctgca gaaaactgct tcatcatgga
     5761 aaatggcgat gttttagcgt taactagcga ttctgcgcgt cctgctgggc attttgtggc
     5821 tggagatgtg tatatcgatg gtagcgggat cggtgatatt ggtaatgtcg ttttacgtga
     5881 ccgtcacctg ctttcagaag aaggtcttgt attagctgtc gcgacagtgg atatcaagaa
     5941 aaaagaaatc ttggctggtc cagatattct ctctcgtgga tttgtctaca tgcgagaatc
     6001 aggagaaatg atcaatgaag ctcagcgaat cttattccac gctttaagag aatgtttgaa
     6061 tggacctgac tgttcagaat ataaattgaa agaagcgatg actggcgcgc ttcagccttt
     6121 cttattcgaa cgtacagaaa gacatccaat gattttgcca atggtaatga caccagatga
     6181 agaataaata agaggttatg aaatcagcac cttgtcctga atataagaga aaacaaatga
     6241 aaaacatgag aagccatttt tgtttgttct tattccttga tacaaggtgg ctctttcata
     6301 gcctcttaaa tttatttact ttttcataca aaaaaatacc tgccacaaga gaaattgtct
     6361 cttgtggcag gtatttttgt tactcgcttt gttctctcgt aaaaattgat gtctaccatc
     6421 aatttgattt acagataaaa ttaagctttt acaacgtttg cagcttgtgg gccgcggtct
     6481 gattcttcaa cgtcgaaagt cactgcttga ccttcttcta atgttttgaa tccgtcacct
     6541 tggatagctg agaagtgaac gaatacgtcg cttccatctt cacgtgagat aaatccaaat
     6601 cctttttcag cgttgaacca ttttactgtt ccgttttcca taataatatc ctcctcgtgc
     6661 caaaagcact aatttaaatt gtaacattgc tcgtgtacga tagaggtgca acagatgttt
     6721 caacttctat tcccaaacaa aattacaata taattgtaca cttgcttcat tttttttgca
     6781 agaaagcaac ttacttctct tccatcattc cttcttctct caaactaaaa tatcgtttgc
     6841 gtccgatgat caagtggtcc agtacagtga tccccatcat ttctccacac ttttgtatcc
     6901 ttttggtaaa attcatatcc tgctgcgatg gaatttcata ttatcaagag taaattacaa
     6961 aagaatcatt taaatcaata ctttcacttt gaatacatgt atttgaagtg gaaggtactt
     7021 atttcaaaaa tagtaaaacc tgtatcttaa attacttaat agtaacataa gatacaggct
     7081 gattttttat tcattgttat ttatactaaa gcacccgata gctctgaaaa caatcacaaa
     7141 ttcaactttt caaagccaca gctttaagtt attttgtcca gacaaccccc attgcccaac
     7201 ccattttatg gaattggcat ccaggcaaca acttttcata taaatcgtaa taattttgtt
     7261 cagataggta cttatctgaa gctaaatgct caagccatga tttagatgtg ttgtgattat
     7321 aaattctaat cgcatttttt attccaaatt tgaaacaatg cggtaaaaac tcttggattg
     7381 cccctaattt ataaacatag gtaggcggtg tctcgacttc agatacatta tcaagaataa
     7441 ctattcttcc ttcctcattc aacagttcct tcatttgctg tattacgctg gctatatcat
     7501 ccaaatgatg aaaggttgtt cggcttacaa taaaatcaaa cttctcatta aaattaagtt
     7561 gttctgcatt catattcaga tagaccgtat ttgttagttg acgtttagat ttggcaagat
     7621 cgagcatttg attagaaata tcaatcccta ccacttcatc ataataactt gctaatttct
     7681 ccactaacaa acccgagcca catccgatat ctaatgctct gcctttcttt ggagacatat
     7741 tagacacaaa gaatgaataa tcattcaaaa gctcattcac gaaatcgtaa tcttctgcaa
     7801 ccttatcaaa ctgtgattct attgtattca aaaagatccc ccattcctac tttatcgaca
     7861 ttctttcatt acttaccact ttagatgttt tttcgttggg gataaaactt ccctttagac
     7921 aattttatcc aaagacaata caacagtgca actttattaa agtcactgtc ctttatcgca
     7981 gcctttactt tttagtaaag acagtggctt ctcttatcaa gtttcaaaac atattatttt
     8041 gaagaaaacg tccatctgaa gtgtcaagtg caaaattaca tataaaggtt tattctaaaa
     8101 tgaaaagatg atacaatcat attcagttac ataaggaggt ttcaattatg tgcaccagta
     8161 tcgcagtagt agaaattact ttatctcatt cataatgaaa aaaatggaaa ggagataaaa
     8221 gtatgggtac tttttctata tttgttatta ataaagttcg ttatcaacca aatcaaaatt
     8281 aattggttat aatgaacgct taatgtcagt tcattataac cagtaaggag aaggttataa
     8341 tgaaccagaa aaaccctaaa gacacgcaaa attttattac ttctaaaaag catgtaaaag
     8401 aaatattgaa tcacacgaat atcagtaaac aagacaacgt aatagaaatc ggatcaggaa
     8461 aaggacattt taccaaagag ctagtcaaaa tgagtcgatc agttactgct atagaaattg
     8521 atggaggctt atgtcaagtg actaaagaag cggtaaaccc ctctgagaat ataaaagtga
     8581 ttcaaacgga tattctaaaa ttttccttcc caaaacatat aaactataag atatatggta
     8641 atattcctta taacatcagt acggatattg tcaaaagaat tacctttgaa agtcaggcta
     8701 aatatagcta tcttatcgtt gagaagggat ttgcgaaaag attgcaaaat ctgcaacgag
     8761 ctttgggttt actattaatg gtggagatgg atataaaaat gctcaaaaaa gtaccaccac
     8821 tatattttca tcctaagcca agtgtagact ctgtattgat tgttcttgaa cgacatcaac
     8881 cattgatttc aaagaaggac tacaaaaagt atcgatcttt tgtttataag tgggtaaacc
     8941 gtgaatatcg tgttcttttc actaaaaacc aattccgaca ggctttgaag catgcaaatg
     9001 tcactaatat taataaacta tcgaaggaac aatttctttc tattttcaat agttacaaat
     9061 tgtttcacta aattaaagta ataaagcgtt ctctaatttc acaagaggac gctttattct
     9121 tcccaaaaat tgttcaatat ttatcaataa atcagtagtt ttaaaagtaa gcacctgtta
     9181 ttgcaataaa attagcctaa ttgagagaag tttctataga atttttcata tacttaacga
     9241 gtgctttcac ctttgaatat agtccttccc acttatcatc acactctccc cgatagcctt
     9301 ttctagctat atccagtaaa gttacatgct ctttaggtaa aagaggtata gcccattctg
     9361 cagcgacatc tttcgaggta atttcaccag tagtcactgt ttgccacatt cgagctaggg
     9421 ttaaaattac attacgctca tcacctttta tcccctcaat tagttctggc aaagaatcct
     9481 taattgctct tcgaatatct gtcaaaggta cggagacaag tatacttgaa gaatcaggac
     9541 caaatagaga aatactattc tttcttgctt gtgctaaaac aatagccaaa tcaggatcat
     9601 agcttggttc ctgaatttgt ccattctcaa attcacccct gagccactca ccgtatataa
     9661 attctctttt tggaggatat tgccaaggga caacttcact cctatttata accgtaactt
     9721 caagtggtct aacagaatcc gtatttccaa tctttcctga tatagtcatt agtctttctg
     9781 ttagtttttt tcgagttaat tgaggtaaac tatgattcac gacgactaga acatctaca
//
LOCUS       DAJSBM010000245         7293 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.103, whole genome shotgun sequence.
ACCESSION   DAJSBM010000245 DAJSBM010000000
VERSION     DAJSBM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.103"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..139)
                     /locus_tag="ONT67_003063"
     CDS             complement(<1..139)
                     /locus_tag="ONT67_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010824811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR2356702.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IA"
     gene            265..1386
                     /locus_tag="ONT67_003064"
     CDS             265..1386
                     /locus_tag="ONT67_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCR2356703.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1644..3146
                     /gene="eat(A)"
                     /locus_tag="ONT67_003065"
     CDS             1644..3146
                     /gene="eat(A)"
                     /locus_tag="ONT67_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCR2356704.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3174..3740
                     /locus_tag="ONT67_003066"
     CDS             3174..3740
                     /locus_tag="ONT67_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR2356705.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3951..5012
                     /locus_tag="ONT67_003067"
     CDS             3951..5012
                     /locus_tag="ONT67_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCR2356706.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            5015..5911
                     /gene="murQ"
                     /locus_tag="ONT67_003068"
     CDS             5015..5911
                     /gene="murQ"
                     /locus_tag="ONT67_003068"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR2356707.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5925..>7293
                     /locus_tag="ONT67_003069"
     CDS             5925..>7293
                     /locus_tag="ONT67_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR2356708.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWL"
BASE COUNT         2431 a         1276 c         1658 g         1928 t
ORIGIN      
        1 cagcaatcaa cccatttgta cgaatggctt caatgtgagc aggccaacga tcgatcaaga
       61 ggacctcatt tccgctttga tgtagcatca atccgattcg gctgcccatt gctccggccc
      121 cagctattgc gattttcatg ttttttctct cctttattca atgagttccc ttttagtata
      181 tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa aatgaaaaaa agtgtcttac
      241 ttaagaagaa agtgagggat acagatggat attttggcgg caattgattc atttaaagga
      301 agtgcttctt catttgaact aaatcaggca gttctaaatg gctgggaaga tcgtaaagga
      361 agaaaaatca atgtgccgat tgcagatggc ggagaaaata ctgcagaagc aatctattac
      421 gcgctcaggg gaacatggcg taccattgat gggatggatc tcttattccg tcagcgaaaa
      481 atcagctatt tattgacaac ttggcaagga aaaaacaccg cagtcatcga atctgcacaa
      541 gcattggggt tagatctact tgataaacca acggatcaaa ctatccgact tgcttccagt
      601 tatgccttag gagaacttat taaagatgca ctgcttcaag aagtagaaca aattattgtc
      661 actttaggag gaagcgggtg tagcgatggt ggtctaggat tactccaatc cttgggtgct
      721 tctttagaag ggtacacaga aggaaatcct ttattatcta ctaaaaaact tcattttgat
      781 cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg ctgcagatgt taccaatcca
      841 tatacaggtc aacaaggagc tgcttggcaa tttggaagac aaaaaggagg aatagatacg
      901 acgctagctt tcttagataa acaagcagga aagattgctc agcaaataaa agatttctgg
      961 gaggtggatt tagatgatat tccaggaagt ggagcagcag gcggtattgg tggagccctt
     1021 tttttgttgg gagcgaagat ggtttctggt ttcgatttag tcagccaaat cactggattg
     1081 gaaaaaaaga tcgaacaagc agatgtcatc attacaggag aagggcggat cgatcagcaa
     1141 acgatccacg gaaaagttcc atacggtgta gcagtgcttg caaaaaaata tcgaaaacca
     1201 ataatcgcta tttgcggaag tagagaccaa gagctagaag gactttctga ttttctccca
     1261 attgtcttaa gtattcattt gggtccaata ggtttagaag aagcaatgga acacaggcaa
     1321 acattagaaa aaacaaaaat cttaggacag acacttagca gatttttcac catcctcccg
     1381 aagtagttga caaaatcatt ttctttatgc tatcgtaatt tcaaatatag gacaaagtga
     1441 gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc tgcattcaga ctgtaccttg
     1501 tggagacaag gaagcagtgt gcagattttc gggattggct tggttttttc gatgtatggg
     1561 tctttttgtg tgtatccaac ccttgcatcc tctttagcca gttctgaaaa taggactggt
     1621 ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat aaaaaatctg acattcggct
     1681 acgacagcca aggcacatta ttatttgaac aagcaaatct aaattttgac acacaatgga
     1741 aactaggact tatcggacga aacggtcgag gaaagacaac tttactgaat attctacaaa
     1801 acaaactacc ttatcaaggg caagtaatcc atcagcaaga atttgcctat ttcccgcaac
     1861 agacaaaaga taaagaacgt ttaacctatt acgtgttaaa tgatattacg gattttgaga
     1921 tatgggaaat cgaaagagag ctccaattga tgcaaacaga tcctgaaatc ttatggagag
     1981 aattcagcac actatcgggg ggagagaaga caaaagtcct actggcactt ttatttgtgg
     2041 atgacactca tttcccgtta atcgatgaac caacgaatca tttggatatc tctggtagaa
     2101 aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt catcgtggtc agccatgacc
     2161 ggggatttat cgatgaagta gtggaccatg ttttagcaat cgaaaaaagt caactggaac
     2221 tttatcaagg gaatttctct atctatgaag aacagaaaaa acttcgtgat gaatttgaaa
     2281 tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct aaagaaaaca gcagctgaaa
     2341 aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa gaaacaagtc ggattcatcg
     2401 atactgaatc tagacgagtg aataaaggag cagtgggtgc tgatgctgca cggacgatga
     2461 aacgatccaa agcaatcgtg aatcggatgg agacccagat cagcgagaaa gaaaaactat
     2521 taaaagatat cgaatatatc gattctttga cgatgaatag ccaagcgtct caccataagc
     2581 gacttttaag cgtagaagat cttcaattag ggtatgaaaa tctgttattc gagccaattc
     2641 attttacaat cgagcctcat cagcgggtgg cgatttcagg tcctaacggt gcaggaaagt
     2701 catccattat ccattatctt ctgggggcat tcaacggcaa ggttatagga gaaaaatacc
     2761 agccaaaaca tctgagcatt agttatgcaa gccaaaatta tgaagacaat cgaggaacgt
     2821 tggcggaatt tgcagagaaa aaccaagtag actaccaagc atttttgaac aacctccgaa
     2881 agcttgggat ggaaagagat gtttttcata acaagatcga gcagatgagt atgggccaac
     2941 ggaaaaaagt ggaattggct aaatctttat cacagccagc tgaactatat acatgggatg
     3001 aaccattgaa ttatttggat gtcttcaatc aagaacaatt agaacaactg atcttgaacg
     3061 tgaaacctgc catgttacta gtggaacatg atcaaacctt cctggataaa gtatctactg
     3121 agattatttc tcttgagaga atctaaccct tgtaatctca agaacaacaa ggtatggtta
     3181 aggggaactt tgccaacgaa ggagagaatc acatgcttta tgtaacgaga cacggagaaa
     3241 caacttggaa cgcacaagga ttagtttgcg gacgggcaga tgtgccgctg actgaaaaag
     3301 gacagatgca ggcacagaaa ctagcagaaa aggtagtaga tttgcctgtt ccaatcacga
     3361 aaatcatcca ttcccctctt caacgagcaa gagatacagc acaagcagtt gctgatcgtc
     3421 tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat ggattttggg gattatgatg
     3481 gactgccaag caaagatgag aactttcaaa aagcacgttt agcgttcgct gtacgattcc
     3541 caaatgggga gtcagtctta gatgtgtatg cacggatcgt tcctttattg aaagaatgca
     3601 tcgaagacga agaaaatgtc tatctgttag tttgccataa tgccttgata agagtgatca
     3661 atgcttattt tcatccgatg cctaatgagg ggttctttac attcatggta gataatacag
     3721 aattggtttc ttatgagtaa ttatgttttt gtgttattta aataaaatat ttcaaggcac
     3781 tgcttttgct tagcagtgcc ttttttaggt tatgacaatt cttttgataa tataaactca
     3841 acctttagaa atgaaattta attattttaa taaaagtatt gaaattttgt ttctttagat
     3901 atatactcta gtcatagaaa tgaagacgga tacagaaaaa gagggataat atgtttggct
     3961 tttcaatatt tatgaacgat tctttgtcag aagagaagaa acagtatatc cgccaaatgg
     4021 catccaatgg ctttgtcggt attttcactt ccatgcatat accagaagat gatgtttcgg
     4081 catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa aaagaataaa ctagaattga
     4141 tggtcgatat ttcaggagag gcattggaac gagcgggttt ttcattagaa gatctaacgc
     4201 ctttgaaagc aattggtgta acaggtttaa gaatggatta tcatatttcc aatcagcaga
     4261 ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc tagcacgatc acgcctaaag
     4321 atatcgatga attgaaagaa gcagaagctg atttctcaca gattgaagct tggcataatt
     4381 attatccacg tcccgaaaca gggctggaca aagaatggta tcagaaaaaa aatcaatggc
     4441 tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg agatacagag ttgagaggcc
     4501 cactatatca aggattgcca actttggaag aacatcgagg gatccatcct cttgcaggcg
     4561 ctctagatct attagcaagt aacacggata ttgtttacat cggagatgca ggtctgtcag
     4621 aaaacgttca ggagcaattt gcttctttcc aaaaagaaca gacagtcttg ctccatacgg
     4681 aacctgtaga tgaagaattt tacgaatata tattagggaa gcatacgaat cggcaagacg
     4741 atgcaagaga tgtcatacga agcgtagatg cacgttttcg agagattccg cctatcccag
     4801 caagaaatac agctacgcgt atgaaagggt ctattacttt agataacgaa aagtatttgc
     4861 gctatatggg tgagatccaa ttaaccaaat atgatttgcc agcggatgaa aaagtgaatg
     4921 tagtagctaa agttatcaaa gaagagctac cattgatcaa ccaaatcaaa gctggtatga
     4981 actatcaatt tataagaaaa gaaggaagat aaaaatgagc aaaatcaatt tagagaacct
     5041 aacaacggaa cgcagaaacc aagaaacttt tggattagat gagatgagta tttctcaagc
     5101 cgttcaacta atgaacaaag aagatcagaa agtggccgct gcggttgaaa aagaactgac
     5161 aagtatcgaa ccagtgattg ctaacacgat tgagtccttt aaaaaaggtg gacgtctgat
     5221 ttatatggga gccggaacaa gtggaagact aggtgtgtta gatgctgctg aatgcgtacc
     5281 gacattcggt gtagaaccgg aaatggtcat aggtctaatt gctggtggag aagaagcaat
     5341 gactgtagct gtcgaagggg ccgaagattc ttatgagcta gggcaagaag atctaatcaa
     5401 gctgaatttg acaaaaaatg atatagttat tggtatcgca gctagcggtc gtacaccata
     5461 tgtgatcggt ggattagatt acgcaaaatc gatcggtgct gtcacaggaa cgatttcttg
     5521 caataaacaa gccgaaatca gtaaacacgc ggatttccca atcgaagtcg atgcaggtcc
     5581 agaatttttg acaggctcta ctcgattgaa gtcaggaaca gcacaaaaac ttatcttgaa
     5641 tatgatttct actatcagta tgattggtat aggtaaagta ttcaataact tgatggtcga
     5701 tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa cgaatcatca tgcaagcgac
     5761 agaagctgat tacgaaacag ctgaaaagta ctttgaagaa gcagatcaaa atgttaagct
     5821 ggctatcgtc atgattttga caaatagtga taaagaggaa gctcaagcaa aattagctga
     5881 agcaaacgga tttgtaaaag ggaccttatg agggggaaaa gaccatggca aaaaacgaac
     5941 tgactttgga agaactggca aaacaaattt ataaaggggc aggcggcatg ggcaatgtag
     6001 aaagcattgt ccactgcatg actcgtgtgc gtatgaagat catcaatgat gatcttgtaa
     6061 atgtctctga attaaaagcg attccaggag tgcttggtgt agtagaagat gaacagcttc
     6121 aagtcatcat cggacctggg aaagtgaaca aagtagcaca gcaaatggtt gatttggcag
     6181 gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc tacagcaaat tcatcagcat
     6241 ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa agcggcacaa aaatcgaaac
     6301 aaaaaccatc taaaatcaaa tcggtattaa aagacatttc taatatcttt gtgccaatga
     6361 ttcctgcatt tgtcggtact gggatcgtgg cagggattgc cgcagtttta gcaaacttag
     6421 tcacagccgg aacacttgat gcagcaacat ggcaacaata tatcgatgtg atgaacatct
     6481 tgaaaaacgg gatgtttagc tacctagtta tctatacagg tatcaatgct gcacaagttt
     6541 ttggtgcaac accaactctt ggtggagtta tcggggccgt tgtgatgctg acagggatga
     6601 atccggaagc accactgaaa aacttattta ctggtgaagc tcttgcagct ggacaaggtg
     6661 ggatcctagg tgtcatcttc gctgtttggc tattgtcaat cgttgagaaa aaactgcata
     6721 aagtagtacc tgatgcggta gatatcatcg ttacaccaac tctttcattg attgccatcg
     6781 gtctgattga aattttcttc atcatgcctt tagcaggttt catttctgat ggcatggtcg
     6841 gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc cggcttcgtt ctaggcacat
     6901 tattcttgcc gatggttatg ttcggactgc atcaaatttt gactccaatc catgtgcaaa
     6961 tgattgatga aactggtcgt acgctattgc ttccaattct tgcgatggct ggtgcaggac
     7021 aagtgggtgc agcattggca ttatggattc gctgcaaaaa agataaagaa ttaacagaaa
     7081 tgatcaaagg agctttacca gtcgggatcc taggaatcgg cgaaccattg atttacggag
     7141 taacattacc tctaggacgc ccatttatta ctgcttgtat cggtggaggt atcggcgggg
     7201 cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat tggaccttca ggagccgctt
     7261 tgatcccatt gatctctgac ggtaaatggt tag
//
LOCUS       DAJSBM010000246         6689 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.106, whole genome shotgun sequence.
ACCESSION   DAJSBM010000246 DAJSBM010000000
VERSION     DAJSBM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.106"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..192
                     /locus_tag="ONT67_003070"
     CDS             <1..192
                     /locus_tag="ONT67_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCR2356709.1"
                     /translation="GAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDETQEGSLERY
                     KKDSYYWYQKLIEENGENL"
     gene            381..2828
                     /locus_tag="ONT67_003071"
     CDS             381..2828
                     /locus_tag="ONT67_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR2356710.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            3058..4125
                     /gene="rlmN"
                     /locus_tag="ONT67_003072"
     CDS             3058..4125
                     /gene="rlmN"
                     /locus_tag="ONT67_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCR2356711.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4318..5421)
                     /locus_tag="ONT67_003073"
     CDS             complement(4318..5421)
                     /locus_tag="ONT67_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCR2356712.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5974..6522
                     /gene="aac(6')"
                     /locus_tag="ONT67_003074"
     CDS             5974..6522
                     /gene="aac(6')"
                     /locus_tag="ONT67_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCR2356713.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2239 a         1141 c         1438 g         1871 t
ORIGIN      
        1 ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag ctggctgaac
       61 ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca agaaggatct
      121 ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga agaaaatgga
      181 gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt
      241 tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa
      301 agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat aatgaaactt
      361 gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga
      421 cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt ggtgttgggt
      481 ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta ggaatattga
      541 ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt cttttattag
      601 ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt aaaaaccgac
      661 gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat gcccatttat
      721 ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat ttgttagcca
      781 gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt ggagcaatcc
      841 tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca gcagtgctat
      901 tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg ttaaatggta
      961 tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc aagcaagctc
     1021 gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc
     1081 gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg
     1141 tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat acagagccgg
     1201 agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct gcgaatctag
     1261 agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt tcagatgagt
     1321 tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag gaaaatcaag
     1381 actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat caaagtgatg
     1441 agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa agtttcggtg
     1501 tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc gaagtccagc
     1561 cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc gcgcttgctt
     1621 tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg attggtattg
     1681 aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa gcacaaccaa
     1741 accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga cgggtacaaa
     1801 cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt agtgggaagt
     1861 cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc catgaagtaa
     1921 aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc atacctcatc
     1981 tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa aaagtagtac
     2041 aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat atttctggtt
     2101 acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca accttaccat
     2161 tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc aatgaagttg
     2221 aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat atgatcctag
     2281 ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat gtaccttcca
     2341 gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat tctaatgggg
     2401 cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag aataaaccga
     2461 tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt caatttgtta
     2521 cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa gttgaaacga
     2581 caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca cttgtcgttg
     2641 aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt tacaaccgag
     2701 cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca gaaggaagca
     2761 agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg gacgataatg
     2821 acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa agacaaggca
     2881 tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt atgatgtctt
     2941 gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag tattgcatta
     3001 aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga aaatactttg
     3061 gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt tatagaaaac
     3121 ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt
     3181 aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc ggagcatttt
     3241 atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac agtaaaatat
     3301 ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca agaatatggc
     3361 ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc
     3421 gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca gatcatgatg
     3481 gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg
     3541 gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca tatcatcaat
     3601 gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag tggattagca
     3661 cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat ttccttgcac
     3721 gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt cccaattgag
     3781 aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat cacgtttgaa
     3841 tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt agcagatcta
     3901 ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc agtcagtgaa
     3961 catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga tgtattgaag
     4021 aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga tgcagcttgc
     4081 ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta tcctactcgt
     4141 tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca acttaaataa
     4201 gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa ccctcatcaa
     4261 gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag caattattta
     4321 actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt ctacgtcaag
     4381 aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa tatcatttgt
     4441 tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat tcatgtcggt
     4501 taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa acaaatcgaa
     4561 ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc cgtgaaggta
     4621 tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt
     4681 atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat ccgaaacgac
     4741 ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt gcgtaccata
     4801 agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat ccgtgtagcc
     4861 tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag catcgccaat
     4921 acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg
     4981 tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt tatagatgac
     5041 acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga agactggttt
     5101 agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac cgactgcaaa
     5161 caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat ttccataagt
     5221 tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca aagctacttt
     5281 tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta cttttgtccc
     5341 atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg tataatttgg
     5401 taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg ggttaaaatt
     5461 ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc taagacagga
     5521 taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc ctaaaaacga
     5581 acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta gtatttctac
     5641 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
     5701 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
     5761 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
     5821 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
     5881 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
     5941 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
     6001 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
     6061 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
     6121 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
     6181 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
     6241 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
     6301 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
     6361 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
     6421 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
     6481 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
     6541 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
     6601 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     6661 aaaagtcgct tagtttcgag tagtaagaa
//
LOCUS       DAJSBM010000247         5873 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.110, whole genome shotgun sequence.
ACCESSION   DAJSBM010000247 DAJSBM010000000
VERSION     DAJSBM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.110"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(41..1027)
                     /locus_tag="ONT67_003075"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="ONT67_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="ONT67_003076"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="ONT67_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCR2356714.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="ONT67_003077"
     CDS             1852..3006
                     /locus_tag="ONT67_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCR2356715.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="ONT67_003078"
     CDS             3221..4189
                     /locus_tag="ONT67_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCR2356716.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="ONT67_003079"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="ONT67_003079"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCR2356717.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="ONT67_003080"
     CDS             5219..5827
                     /locus_tag="ONT67_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCR2356718.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1772 a         1144 c         1320 g         1637 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaacggtt ctgttgcaaa gttttaaatc tactatcaaa taa
//
LOCUS       DAJSBM010000248         3694 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.140, whole genome shotgun sequence.
ACCESSION   DAJSBM010000248 DAJSBM010000000
VERSION     DAJSBM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.140"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1632
                     /locus_tag="ONT67_003081"
     CDS             <1..1632
                     /locus_tag="ONT67_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR2356719.1"
                     /translation="AKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTY
                     LTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGEEWDKDVWDR
                     RNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKE
                     FFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLE
                     WDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMR
                     ISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPD
                     SEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNG
                     MPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDD
                     VELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERT
                     EELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1639..2016
                     /locus_tag="ONT67_003082"
     CDS             1639..2016
                     /locus_tag="ONT67_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR2356720.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2170..2952
                     /locus_tag="ONT67_003083"
     CDS             2170..2952
                     /locus_tag="ONT67_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HCR2356721.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(3078..>3694)
                     /gene="erm"
                     /locus_tag="ONT67_003084"
     CDS             complement(3078..>3694)
                     /gene="erm"
                     /locus_tag="ONT67_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HCR2356722.1"
                     /translation="GKGHFTKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQT
                     DILKFSFPKHINYKIYGNIPYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRA
                     LGLLLMVEMDIKMLKKVPPLYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWV
                     NREYRVLFTKNQFRQALKHANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT         1340 a          536 c          707 g         1111 t
ORIGIN      
        1 gcaaaaactg tctggattag aaaaggaaca gtaattaata aaatgttaga tttcttagat
       61 ttaaagtatc ctagcattac ttcaattact gaagttccta ttgaaaaagc aatgacggag
      121 tatagaactt atttgacaaa acggggtgtt agaattacca ctactaatta taagattact
      181 gctaatcaag aaaaaacacc tgtaaaagct aattcctact atgttactaa tctaaaacaa
      241 tttatggagt tttatgagaa cttttatttt gatggagagg agtgggataa agacgtttgg
      301 gatagacgta acttaccttt gccagatgat aaggttaacc caacacaata tgaatataca
      361 attaacttta aagggtttcg gaatacatat tttaaacaac ttgtaaaaag atattgtaag
      421 ttgagattga acatggatag cttttcctat gtaagtgata ttgcccaaag acttaaagag
      481 ttctttaatt ttctggacat gaaatttaaa caagttcaga gagtacacca attaacgaga
      541 gtggaaattg aagcatattt aagtgaacta aacatgatgg gaataaaacc tagtacaata
      601 actgggagga tctctatatt ggaaggacta tttagtaccc ttcttaggct agaatgggat
      661 gatgttcctt ccaaaatatt aatttattct gaggactatc cgaaaatacc aagagcaaaa
      721 ccacgcttta tagatgaatt cgtcctagag caattgaaca gtcatcttga taaattaccc
      781 gaatatatag ctacgatgac tatgattgtt caagaatgtg gaatgaggat aagtgaattg
      841 tgcaccttga aaaaaggctg tctattagag gacaaagatg gagatttctt tttaaagtat
      901 tatcaatgga aaatgaaaaa ggagcatata gttccaatat ctaaagaggt agctttactt
      961 attaaagttc gggaagataa agtttcagag gaatttccag atagtgaata cctctttcca
     1021 agaaaagatg gatcgccatt aaaacaagaa acatttagag gtgagttaaa taaattagct
     1081 tatgagcaaa atatagtgga taaatcaggt gagatttata gattccatgc ccatgccttt
     1141 cgccatacag taggaacaag aatgattaac aacgggatgc cccagcatat tgtgcagaaa
     1201 tttttggggc atgaaagccc agaaatgaca agcagatacg ctcatatctt tgatgaaact
     1261 ctaaaaaatg aatttactaa atttcaggaa aaactggtta ccaataatgg agatgtgctt
     1321 gatctagatg aagataatga agtcgatgat gtagagcttc aatggttcaa gaaaaatata
     1381 aatgcacaag tgcttccaaa tggttattgt agattgccag tagtagcagg tggttgtcca
     1441 catgcgaatg catgcttaga ttgcactcac ttctgtacca gtaagcaatt cttaccacag
     1501 cacgaagaac agttagagcg tacagaagag ttattagcca tagctaagga taaacaatgg
     1561 caaagacaag tagagactaa tagccgtgtt aaagagcgtt tagaacaaat cattggaagt
     1621 ttgacggggt aattatcaat ggataaacaa gttagaaata caacagaaat tgtacgtttg
     1681 gcgaagcaga aatcaaaaaa gacaagggaa aaagtagaca aagcgatttc taaattttcg
     1741 attgaaggta aagttattaa ttttaattca atagcaaagg aagctaatgt ttctaaatca
     1801 tggctttata aggaacacga tattaggcaa agaatcgaat cccttcgtga gcgtcaaata
     1861 acagcaaatg tagtctcaaa acccaagaaa agttctcgtt cggaggaaat ccttattaaa
     1921 accttaaaaa gaagagtaat ggaattagaa aaagaaaata aaaaattaca gaaccaaatt
     1981 caaaaattat atggagatct gtataataaa gaataattat taatctgtag acaaattgtg
     2041 aaaggatgta cttaaacgct aacggtcagc tttattgaac agtcatttag gtatatgtcc
     2101 aatctagggt aagtaaattg agtatcaata taaactttat atgaacataa tcagttacga
     2161 ggtgaaatca tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta
     2221 aaaatcgtaa aagatttatt tggaagttca atagttggag tatatctatt tggttcagca
     2281 gtaaatggtg gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt
     2341 ttacctcaat taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt
     2401 ggaaatacgg attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc
     2461 ccttggcaat atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt
     2521 gagaatggac aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa
     2581 gcaagaaaga atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta
     2641 cctttgacag atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata
     2701 aaaggtgatg agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact
     2761 ggtgaaatta cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag
     2821 catgtaactt tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg
     2881 gaaggactat attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact
     2941 tctctcaatt aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt
     3001 attgataaat attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa
     3061 cgctttatta ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt
     3121 tccttcgata gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg
     3181 tttttagtga aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac
     3241 tttttgtagt ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag
     3301 tctacacttg gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc
     3361 atctccacca ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat
     3421 cccttctcaa cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca
     3481 atatccgtac tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg
     3541 aaggaaaatt ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct
     3601 tctttagtca cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt
     3661 ttgactagct ctttggtaaa atgtcctttt cctg
//
LOCUS       DAJSBM010000249         3419 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.145, whole genome shotgun sequence.
ACCESSION   DAJSBM010000249 DAJSBM010000000
VERSION     DAJSBM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.145"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..164)
                     /locus_tag="ONT67_003085"
     CDS             complement(<1..164)
                     /locus_tag="ONT67_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2356723.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="ONT67_003086"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="ONT67_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2356724.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRKIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="ONT67_003087"
     CDS             complement(963..2915)
                     /locus_tag="ONT67_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2356725.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3158)
                     /locus_tag="ONT67_003088"
     CDS             complement(2922..3158)
                     /locus_tag="ONT67_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356726.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1056 a          590 c          556 g         1217 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aagattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tcttttttt
//
LOCUS       DAJSBM010000250         3419 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.146, whole genome shotgun sequence.
ACCESSION   DAJSBM010000250 DAJSBM010000000
VERSION     DAJSBM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.146"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..164)
                     /locus_tag="ONT67_003089"
     CDS             complement(<1..164)
                     /locus_tag="ONT67_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2356727.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="ONT67_003090"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="ONT67_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCR2356728.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..2915)
                     /locus_tag="ONT67_003091"
     CDS             complement(963..2915)
                     /locus_tag="ONT67_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCR2356729.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2922..3158)
                     /locus_tag="ONT67_003092"
     CDS             complement(2922..3158)
                     /locus_tag="ONT67_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR2356730.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1056 a          590 c          555 g         1218 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ctgaaaccct tccaacaacc atcttagcga acattggaag aatggtgttc
     2281 ttttgttggt gataagtagt ggtttcttct ctttttggta gaacattagt gaacttctta
     2341 cacgccttac attgatagcg ttgagatttg cccctacttt tccccctttt gtaaaattgc
     2401 ttcggctcat tgaatggggt tgattcttct tccgagcagc cttctttatg aaattgataa
     2461 tctggctcaa catcttggat gctattgatt tctataaggc gtttaatttc ttcaacaaca
     2521 gaccaattag ataaaggggt tactgtacag ttttgcgata cacctctgcc aataggattg
     2581 tcattgcagt acataccttt atgaccttta tctttggaac taccagtcat tttatatcga
     2641 cttggtttcc cttttacatc tttgaatttg tgctgttctt tcccaaaatt acaacaaaac
     2701 ggattgatgc agtaattata ttgaatggga tattcagaac cgttccattt aaaagacgtt
     2761 ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt ttggagttaa taaatcatat
     2821 tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa ttttaacatc tacaatttca
     2881 tcttccttcg tggctaatct tttaagcttg gtcattgtct atcaacccac tctatctttg
     2941 atacccattt tttcaacaat ccgcttatta cttcgcttgt tggcaatcca ttcaataaac
     3001 ttcttatcgt ctaaaatgtt caataagaat tcatccactg cttcgctttc agtacgctct
     3061 gcatattcag cgtattgctt aactatcacc cttacttgct ctgatatttc ccaatccaca
     3121 gacttagcgt tcttattttt tggctcaaga aattgcatag gaaaatgcac ccctttttgc
     3181 ttagtaaatt gtaaccattt taacacacca atttgcattc ctacaataat tatttcacgt
     3241 tcgcattttt atatacattt taacttacat tttgtggtgt aaattaacaa caaacgttga
     3301 tttaacaata aaaaacgagt atcaaattca gttgcaattt gatactcatt taggtttaca
     3361 ttttcacttt ttgtactcca aaactgaact acttaatatg caagacgttg tcttttttt
//
LOCUS       DAJSBM010000251         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.173, whole genome shotgun sequence.
ACCESSION   DAJSBM010000251 DAJSBM010000000
VERSION     DAJSBM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.173"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003093"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2356731.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT67_003094"
     CDS             2060..2227
                     /locus_tag="ONT67_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356732.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT67_003095"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT67_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356733.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          817 a          361 c          483 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBM010000252         2371 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.174, whole genome shotgun sequence.
ACCESSION   DAJSBM010000252 DAJSBM010000000
VERSION     DAJSBM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.174"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003096"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2356734.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2060..2227
                     /locus_tag="ONT67_003097"
     CDS             2060..2227
                     /locus_tag="ONT67_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356735.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2287..>2371)
                     /locus_tag="ONT67_003098"
     CDS             complement(2287..>2371)
                     /locus_tag="ONT67_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356736.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          816 a          361 c          484 g          710 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
     1261 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
     1321 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1381 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1441 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1501 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1861 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1921 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1981 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     2041 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     2101 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     2161 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     2221 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     2281 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     2341 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBM010000253         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.175, whole genome shotgun sequence.
ACCESSION   DAJSBM010000253 DAJSBM010000000
VERSION     DAJSBM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.175"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003099"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2356737.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT67_003100"
     CDS             2039..2227
                     /locus_tag="ONT67_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356738.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT67_003101"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT67_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356739.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          814 a          363 c          469 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSBM010000254         2369 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.176, whole genome shotgun sequence.
ACCESSION   DAJSBM010000254 DAJSBM010000000
VERSION     DAJSBM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.176"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003102"
     CDS             22..1941
                     /gene="tet(M)"
                     /locus_tag="ONT67_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HCR2356740.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLDSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2039..2227
                     /locus_tag="ONT67_003103"
     CDS             2039..2227
                     /locus_tag="ONT67_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356741.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2285..>2369)
                     /locus_tag="ONT67_003104"
     CDS             complement(2285..>2369)
                     /locus_tag="ONT67_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356742.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          813 a          363 c          470 g          723 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatac gtctgggaaa
      601 ttattggaag cattagaact cgaacaagag gaaagcataa gatttcataa ttgttccctg
      661 ttccctgttt atcacggaag tgcaaaaaac aatataggga ttgataacct tatagaagtg
      721 attacgaata aattttattc atcaacacat cgaggtcagt ctgaactttg cggaaaagtt
      781 ttcaaaattg agtattcgga aaaaagacag cgtcttgcat atatacgtct ttatagtggc
      841 gtactgcatt tgcgagattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaatcgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagtta gatagtgttc ttggagatac aaaactattg
     1021 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
     1081 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
     1141 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1561 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1681 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1741 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1801 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1861 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1921 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1981 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     2041 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     2101 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     2161 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     2221 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     2281 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     2341 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSBM010000255         2011 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.183, whole genome shotgun sequence.
ACCESSION   DAJSBM010000255 DAJSBM010000000
VERSION     DAJSBM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.183"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..179)
                     /locus_tag="ONT67_003105"
     CDS             complement(<1..179)
                     /locus_tag="ONT67_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2356743.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRR"
     gene            541..1581
                     /locus_tag="ONT67_003106"
     CDS             541..1581
                     /locus_tag="ONT67_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2356744.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAQLP
                     LGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNKIT"
     gene            1700..1867
                     /locus_tag="ONT67_003107"
     CDS             1700..1867
                     /locus_tag="ONT67_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356745.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(1927..>2011)
                     /locus_tag="ONT67_003108"
     CDS             complement(1927..>2011)
                     /locus_tag="ONT67_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356746.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          661 a          327 c          358 g          665 t
ORIGIN      
        1 tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc
       61 ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg
      121 tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt
      181 tataacacag cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt
      241 ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata
      301 ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat
      361 tttgtatgta atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt
      421 ttgtcatttt gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga
      481 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      541 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      601 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
      661 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
      721 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
      781 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
      841 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaaata
      901 aaagagccta cagtcattta tatggaaaga ccgttaaaaa aagcagagta taccattcac
      961 atcgaagttc caccgaatcc tttctgggct tccattggtc tatctgtagc acagcttcca
     1021 ttagggagcg gagtacagta tgagagctcg gtttctcttg gatacttaaa tcaatcgttt
     1081 caaaatgcag ttatggaggg gatacgctat ggctgtgaac aaggattgta tggttggaat
     1141 gtgacggact gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1201 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1261 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1321 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1381 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1441 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact
     1501 accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat
     1561 atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa
     1621 ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat
     1681 tataaactac aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag
     1741 ggaagatact gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt
     1801 aattgaaata aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag
     1861 ccgataaaat gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc
     1921 ttttaattag tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa
     1981 ggatagttct ttgtctgtaa agctatccat g
//
LOCUS       DAJSBM010000256         2009 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.184, whole genome shotgun sequence.
ACCESSION   DAJSBM010000256 DAJSBM010000000
VERSION     DAJSBM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.184"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            complement(<1..179)
                     /locus_tag="ONT67_003109"
     CDS             complement(<1..179)
                     /locus_tag="ONT67_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR2356747.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRR"
     gene            541..1581
                     /locus_tag="ONT67_003110"
     CDS             541..1581
                     /locus_tag="ONT67_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR2356748.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1679..1867
                     /locus_tag="ONT67_003111"
     CDS             1679..1867
                     /locus_tag="ONT67_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCR2356749.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1925..>2009)
                     /locus_tag="ONT67_003112"
     CDS             complement(1925..>2009)
                     /locus_tag="ONT67_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCR2356750.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          658 a          329 c          344 g          678 t
ORIGIN      
        1 tttcttctgt caggtaaggc tcttccgatt gattcaaggt tcttcctacc taatattatc
       61 ggatgtccct ttgtagtatt tttaacatat tcccagtcct tgggaatcct ccaaggaatg
      121 tcattctctt tgccaatcac tctattctta tccatcgcag caatcaaaga aactttcatt
      181 tataacacag cctttttaac taatttacca tctaatttct tatttctatc aaaaaacctt
      241 ttctcattat cctgcccgtt agttgaataa gcaatttttt ctataagtta tattatcata
      301 ataccacaaa atcacaaatt ttcattcaat tatcattttc aattgcattt tataaatcat
      361 tttgtatgta atttggtatg taagttaaaa tgaaaaaagt agtccatttt gaagtacatt
      421 ttgtcatttt gtactccaaa actgaactac ttacttgcat atatacgcct ttatagtgga
      481 gtactacatt tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa
      541 atgtatactt caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt
      601 gttattttgc aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg
      661 ccacagagaa aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt
      721 aaacctgaac agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg
      781 cttctacgat attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
      841 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
      901 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
      961 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1021 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1081 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1141 gtgacggatt gtaaaatctg ttttaagtac ggtttatact atagccctgt tagtactcca
     1201 gcagattttc ggatgcttac tcctattgta ctggagcaag cctttagaaa agctggaaca
     1261 gaattgttag agccatatct tagttttaaa gtttatgcac cacaggaata tctttcacgg
     1321 gcatataacg atgctcccaa atattgtgca aatatcgtaa atactcaact gaaaaataat
     1381 gaggtcatta ttattggaga aattcctgct cgatgtattc aagattatcg caatgattta
     1441 acttttttta caaatgggct tagtgtttgt ttagcagagc taaaaggata tcaggttacc
     1501 actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc ggatagataa agtaagatat
     1561 atgttcaata aaataactta gtgcgtttta tgttgttata taaatatggt ttcttattaa
     1621 ataagatgaa atattcttta atatagattt gaattaaagt ggaaaggagg agattgttat
     1681 tataaactac aagtggatat tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg
     1741 agtggatact atacttaaaa atttcccttt atactgcccc aaatgtaaga acgaaacttt
     1801 aattaatgtt caaaaaatga atataataac aatcaaagag ccagacgcca agacgcagag
     1861 ccgataattt gagaaatgaa actctcatct tatcggctct ttttgtttat ctgaatttta
     1921 ctgactagcc ttcaatattt cttgcttcat tgataccttt tgctagtgct tccattaagg
     1981 atagttcttt gtctgtaaag ctatccatg
//
LOCUS       DAJSBM010000257         1781 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.188, whole genome shotgun sequence.
ACCESSION   DAJSBM010000257 DAJSBM010000000
VERSION     DAJSBM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.188"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            24..416
                     /locus_tag="ONT67_003113"
     CDS             24..416
                     /locus_tag="ONT67_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR2356751.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT67_003114"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="ONT67_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR2356752.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DAJSBM010000258         1504 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.195, whole genome shotgun sequence.
ACCESSION   DAJSBM010000258 DAJSBM010000000
VERSION     DAJSBM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.195"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            262..345
                     /locus_tag="ONT67_003115"
                     /pseudo
     CDS             262..345
                     /locus_tag="ONT67_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="ONT67_003116"
     CDS             394..477
                     /locus_tag="ONT67_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCR2356753.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT67_003117"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="ONT67_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCR2356754.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAJSBM010000259         1382 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.202, whole genome shotgun sequence.
ACCESSION   DAJSBM010000259 DAJSBM010000000
VERSION     DAJSBM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.202"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            <1..1311
                     /locus_tag="ONT67_003118"
     CDS             <1..1311
                     /locus_tag="ONT67_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HCR2356755.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DAJSBM010000260         1350 bp    DNA     linear   BCT 01-NOV-2022
DEFINITION  TPA_asm: Enterococcus faecium isolate VRED13-03
            SAMN30692146-rid15770073.guided.203, whole genome shotgun sequence.
ACCESSION   DAJSBM010000260 DAJSBM010000000
VERSION     DAJSBM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN30692146
            Sequence Read Archive: SRR21444931
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 01-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001472590.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001472590.1
            Genome Coverage       :: 102x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/02/2022 01:28:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,120
            CDSs (total)                      :: 3,058
            Genes (coding)                    :: 2,984
            CDSs (with protein)               :: 2,984
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 22 of 74
            Pseudo Genes (incomplete)         :: 42 of 74
            Pseudo Genes (internal stop)      :: 17 of 74
            Pseudo Genes (multiple problems)  :: 6 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN30692146-rid15770073.guided.203"
                     /isolate="VRED13-03"
                     /isolation_source="gastrointestinal tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="United Kingdom: Edinburgh"
                     /lat_lon="55.57 N 3.11 W"
                     /collection_date="2017"
                     /collected_by="NHS Lothian"
                     /note="genotype: vanA"
     gene            47..298
                     /locus_tag="ONT67_003119"
                     /pseudo
     CDS             47..298
                     /locus_tag="ONT67_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="ONT67_003120"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="ONT67_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HCR2356756.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
